Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| cyclobenzaprine raises ros levels in leishmania infantum and reduces parasite burden in infected mice. | the leishmanicidal action of tricyclic antidepressants has been studied and evidences have pointed that their action is linked to inhibition of trypanothione reductase, a key enzyme in the redox metabolism of pathogenic trypanosomes. cyclobenzaprine (cbp) is a tricyclic structurally related to the antidepressant amitriptyline, differing only by the presence of a double bond in the central ring. this paper describes the effect of cbp in experimental visceral leishmaniasis, its inhibitory effect i ... | 2017 | 28045892 |
| a recombinant fusion protein displaying murine and human mhc class i- and ii-specific epitopes protects against leishmania amazonensis infection. | tegumentary leishmaniasis (tl) constitutes a major public health problem with significant morbidity worldwide. synthetic peptide-based vaccines are attractive candidates to protect against leishmaniasis, since t cell-specific epitopes can be delivery to antigen-presenting cells, leading to the generation of a th1 cell-mediated immunity. in this context, the present study aims to evaluate the immunogenicity and protective efficacy of a vaccine composed of major histocompatibility complex class i ... | 2017 | 28049560 |
| comprehensive candidate gene analysis for symptomatic or asymptomatic outcomes of leishmania infantum infection in brazil. | genetic risk factors contribute to asymptomatic versus symptomatic visceral leishmaniasis (vl) outcomes following infection with leishmania infantum. we therefore carried out a family-based (n = 918 post-quality control fully genotyped and phenotyped individuals) candidate gene study for symptomatic vl or asymptomatic delayed-type hypersensitivity (dth) skin test phenotypes in highly endemic neighborhoods of northeast brazil. a total of 248 snps were genotyped in 42 genes selected as candidates ... | 2017 | 28054334 |
| antileishmanial activity and immunomodulatory effects of tricin isolated from leaves of casearia arborea (salicaceae). | bioactivity-guided fractionation of antileishmanial active extract from leaves of casearia arborea led to isolation of three metabolites: tricin (1), 1',6'-di-o-β-d-vanilloyl glucopyranoside (2) and vanillic acid (3). compound 1 demonstrated the highest activity against the intracellular amastigotes of leishmania infantum, with an ic50 value of 56 μm. tricin (1) demonstrated selectivity in mammalian cells (si > 7) and elicited immunomodulatory effect on host cells. the present work suggests that ... | 2017 | 28054741 |
| in vitro antiprotozoal activity of (s)-cis-verbenol against leishmania spp. and trypanosoma cruzi. | (s)-cis-verbenol, a monoterpene frequently found as a component of essential oils, was assayed against leishmania amazonensis, leishmania infantum, leishmania brasiliensis and against two strains of trypanosoma cruzi. the cytotoxicity of the compound was also assayed against human fibroblast cells using a colorimetric method. benznidazole was used as reference drug against t. cruzi and amphotericin b was used against leishmania spp. the compound showed good activity against the trypanosomes, bei ... | 2017 | 28062234 |
| methoxylated 2'-hydroxychalcones as antiparasitic hit compounds. | chalcones display a broad spectrum of pharmacological activities. herein, a series of 2'-hydroxy methoxylated chalcones was synthesized and evaluated towards trypanosoma brucei, trypanosoma cruzi and leishmania infantum. among the synthesized library, compounds 1, 3, 4, 7 and 8 were the most potent and selective anti-t. brucei compounds (ec50 = 1.3-4.2 μm, selectivity index >10-fold). compound 4 showed the best early-tox and antiparasitic profile. the pharmacokinetic studies of compound 4 in bal ... | 2017 | 28064141 |
| the effects of increased heme oxygenase-1 on the lymphoproliferative response in dogs with visceral leishmaniasis. | canine visceral leishmaniasis (cvl) is known to affect the cellular immunity of infected dogs, through impairing lymphoproliferation and microbicidal mechanisms. this study examined heme oxygenase-1 (ho-1) and its metabolites, oxidative stress and il-10 levels in cvl and investigated correlations between these parameters. additionally, the effects of ho-1 inhibition on the lymphoproliferative response and cytokine production in lymph node cells (lncs) from infected dogs were evaluated. forty-fou ... | 2017 | 28065450 |
| development and evaluation of zinc phthalocyanine nanoemulsions for use in photodynamic therapy for leishmania spp. | photodynamic therapy (pdt) combines light with photosensitizers (ps) for production of reactive oxygen species (ros) that can kill infectious microorganisms such as bacteria, fungi and protozoa. the application of nanotechnology has enabled the advancement of pdt because many ps are insoluble in water, necessitating a nanocarrier as a physiologically acceptable carrier. nanoemulsions are efficient nanocarriers for solubilizing liposoluble drugs, like the ps, in water. cutaneous (cl) and mucocuta ... | 2017 | 28071592 |
| vector competence of lutzomyia cruzi naturally demonstrated for leishmania infantum and suspected for leishmania amazonensis. | corumbá city is one of the oldest visceral leishmaniasis-endemic foci in the state of mato grosso do sul, brazil, where the transmission of leishmania infantum has been attributed to lutzomyia cruzi aiming at investigating the parameters of the vectorial capacity of lu. cruzi for l. infantum, a project was undertaken in this city. among these parameters, vector competence was investigated and the results obtained are reported herein. of the 12 hamsters exposed to feed wild-caught female sandflie ... | 2017 | 28077746 |
| nycthemeral rhythm of phlebotominae (diptera: psychodidae) in a craggy region, transitioning between the wetland and the plateau, brazil. | recording the nycthemeral rhythm of sand flies allows the evaluation of the daily activity in different ecotypes, the period of greatest activity, and their degree of anthropophily. we investigated the fauna and the rhythm of sand fly activity in an ecotourism region in mato grosso do sul (ms) state, brazil. sand flies were captured monthly, using a shannon trap for 24 h periods between july 2012 and june 2014. we collected 1,815 sand flies, in which lutzomyia whitmani (=nyssomyia whitmani, sens ... | 2017 | 28082638 |
| the gut microbiome of the vector lutzomyia longipalpis is essential for survival of leishmania infantum. | the vector-borne disease leishmaniasis, caused by leishmania species protozoa, is transmitted to humans by phlebotomine sand flies. development of leishmania to infective metacyclic promastigotes in the insect gut, a process termed metacyclogenesis, is an essential prerequisite for transmission. based on the hypothesis that vector gut microbiota influence the development of virulent parasites, we sequenced midgut microbiomes in the sand fly lutzomyia longipalpis with or without leishmania infant ... | 2017 | 28096483 |
| inhibition of leishmania infantum trypanothione reductase by diaryl sulfide derivatives. | the study presented here aimed at identifying a new class of compounds acting against leishmania parasites, the causative agent of leishmaniasis. for this purpose, the thioether derivatives of our in-house library have been evaluated in whole-cell screening assays in order to determine their in vitro activity against leishmania protozoan. among them, promising results have been achieved with compound rds 777 (6-(sec-butoxy)-2-((3-chlorophenyl)thio)pyrimidin-4-amine) (ic50 = 29.43 µm), which is a ... | 2017 | 28098499 |
| antileishmanial activities of caffeic acid phenethyl ester loaded plga nanoparticles against leishmania infantum promastigotes and amastigotes in vitro. | to investigate and compare the antileishmanial effects of cape and (cape)plga nps on leishmania infantum (l. infantum) promastigotes and amastigotes in vitro. | 2017 | 28107861 |
| identification of balb/c immune markers correlated with a partial protection to leishmania infantum after vaccination with a rationally designed multi-epitope cysteine protease a peptide-based nanovaccine. | through their increased potential to be engaged and processed by dendritic cells (dcs), nanovaccines consisting of poly(d,l-lactic-co-glycolic acid) (plga) nanoparticles (nps) loaded with both antigenic moieties and adjuvants are attractive candidates for triggering specific defense mechanisms against intracellular pathogens. the aim of the present study was to evaluate the immunogenicity and prophylactic potential of a rationally designed multi-epitope peptide of leishmania cysteine protease a ... | 2017 | 28114333 |
| prediction of lymph node parasite load from clinical data in dogs with leishmaniasis: an application of radial basis artificial neural networks. | quantification of leishmania infantum load via real-time quantitative polymerase chain reaction (qpcr) in lymph node aspirates is an accurate tool for diagnostics, surveillance and therapeutics follow-up in dogs with leishmaniasis. however, qpcr requires infrastructure and technical training that is not always available commercially or in public services. here, we used a machine learning technique, namely radial basis artificial neural network, to assess whether parasite load could be learned fr ... | 2017 | 28115177 |
| antileishmanial activity of meroditerpenoids from the macroalgae cystoseira baccata. | the development of novel drugs for the treatment of leishmaniases continues to be crucial to overcome the severe impacts of these diseases on human and animal health. several bioactivities have been described in extracts from macroalgae belonging to the cystoseira genus. however, none of the studies has reported the chemical compounds responsible for the antileishmanial activity observed upon incubation of the parasite with the aforementioned extracts. thus, this work aimed to isolate and charac ... | 2017 | 28126391 |
| canine visceral leishmaniasis in the metropolitan area of são paulo: pintomyia fischeri as potential vector of leishmania infantum. | american visceral leishmaniasis is a zoonosis caused by leishmania infantum and transmitted mainly by lutzomyia longipalpis. however, canine cases have been reported in the absence of this species in the greater são paulo region, where pintomyia fischeri and migonemyia migonei are the predominant species. this raises the suspicion that they could be acting as vectors. therefore, this study sought to investigate specific vector capacity parameters of these species and to compare them with those o ... | 2017 | 28134092 |
| intestinal parasites and vector-borne pathogens in stray and free-roaming cats living in continental and insular greece. | this survey investigated the distribution of various intestinal parasites and vector-borne pathogens in stray and free-roaming cats living in four regions of greece. a total number of one hundred and fifty cats living in three islands (crete, mykonos and skopelos) and in athens municipality was established as a realistic aim to be accomplished in the study areas. all cats were examined with different microscopic, serological and molecular assays aiming at evaluating the occurrence of intestinal ... | 2017 | 28141857 |
| parasitism and inflammation in ear skin and in genital tissues of symptomatic and asymptomatic male dogs with visceral leishmaniasis. | canine visceral leishmaniasis (cvl) is transmitted through vector, although venereal transmission has been suggested. this study aimed to compare the parasitic loads and inflammatory processes in genital tissues with ear skin from seropositive male dogs. forty-five seropositive dogs were separated into groups containing symptomatic (n = 23) and asymptomatic (n = 22) animals. the control group (n = 2) healthy animals with seronegative and negative results in direct parasitological test. samples o ... | 2017 | 28160074 |
| development and validation of a pcr-elisa for the diagnosis of symptomatic and asymptomatic infection by leishmania (leishmania) infantum. | a kdna pcr enzyme-linked immunosorbent assay (kdna pcr-elisa) for the diagnosis of human visceral leishmaniasis (hvl) was developed. the detection limit of the reaction, precision measurements, and cut-off of the kdna pcr-elisa were defined in a proof-of-concept phase. a reference strain of leishmania (leishmania) infantum and a bank of 14 peripheral blood samples from immunocompetent patients with vl were characterized using techniques considered gold standards, and 11 blood samples obtained fr ... | 2017 | 28163725 |
| risk analysis and prediction of visceral leishmaniasis dispersion in são paulo state, brazil. | visceral leishmaniasis (vl) is an important neglected disease caused by a protozoan parasite, and represents a serious public health problem in many parts of the world. it is zoonotic in europe and latin america, where infected dogs constitute the main domestic reservoir for the parasite and play a key role in vl transmission to humans. in brazil this disease is caused by the protozoan leishmania infantum chagasi, and is transmitted by the sand fly lutzomyia longipalpis. despite programs aimed a ... | 2017 | 28166251 |
| nanoliposomal buparvaquone immunomodulates leishmania infantum-infected macrophages and is highly effective in a murine model. | visceral leishmaniasis is a fatal parasitic neglected disease affecting 1.5 million people worldwide. based on a drug repositioning approach, the aim of this work was to investigate the in vitro immunomodulatory potential of buparvaquone (bpq) and to establish a safe regimen to evaluate the in vivo efficacy of bpq entrapped by negatively charged nanoliposomes (bpq-lp) in leishmania infantum-infected hamsters. small-angle x-ray scattering, dynamic light scattering, and the ζ-potential were applie ... | 2017 | 28167544 |
| leishmania infantum and epstein-barr virus co-infection in a patient with hemophagocytosis. | the authors describe a rare case of a 27- year old previously healthy male presenting with high grade fever, pancytopenia, hepatosplenomegaly, high levels of ferritin and triglyceride, suggesting a diagnosis of hemophagocytic lymphohistiocytosis (hlh) syndrome. other investigations showed a positive leishmania infantum serology and high epstein-barr virus (ebv) viremia. the diagnosis of a visceral leishmaniasis was confirmed by bone morrow biopsy, which showed leishman-donovan bodies and evidenc ... | 2016 | 28191297 |
| intraspecific genetic variability in a population of moroccan leishmania infantum revealed by pcr-rflp of kdna minicircles. | in morocco, leishmania infantum is the main etiologic agent of human and canine visceral leishmaniasis (vl). this species has been proven to be an opportunistic agent in hiv+ patients and is also responsible of sporadic cutaneous leishmaniasis (cl).this work aims to evaluate the genetic variability of moroccan l. infantum strains based on pcr-rflp analysis of the kinetoplastid dna (kdna) minicircles. a total of 75 dna samples extracted from positive giemsa-stained smears (n=32) and from l. infan ... | 2017 | 28209553 |
| autochthonous outbreak and expansion of canine visceral leishmaniasis, uruguay. | we report an outbreak of canine visceral leishmaniasis in uruguay. blood specimens from 11/45 dogs tested positive for leishmania spp. specimens of lutzomyia longipalpis sand flies were captured; typing revealed leishmania infantum. our findings document an expansion of visceral leishmaniasis to southern south america and risk for vectorborne transmission to humans. | 2017 | 28221113 |
| ultrastructural findings from paraffin embedded tissue in intraoral lesions caused by leishmania infantum. | 2017 | 28230510 | |
| detection of leishmania spp. in bats from an area of brazil endemic for visceral leishmaniasis. | the multihost parasites leishmania spp. infect a broad range of wild mammalian species including bats. several species of bats have adapted to a variety of food resources and shelters in urban areas. this study aimed to detect leishmania spp. dna in bats present in forest fragments located in metropolitan areas endemic for leishmaniasis in campo grande, mato grosso do sul (ms), brazil. blood samples were obtained from 80 individuals, including eight species of phyllostomidae and one species of v ... | 2017 | 28233434 |
| experimental infection and transmission of leishmania by lutzomyia cruzi (diptera: psychodidae): aspects of the ecology of parasite-vector interactions. | several parameters should be addressed before incriminating a vector for leishmania transmission. those may include its ability to become infected by the same leishmania species found in humans, the degree of attractiveness for reservoirs and humans and capacity to sustain parasite infection under laboratory conditions. this study evaluated the vectorial capacity of lutzomyia cruzi for leishmania infantum and gathered information on its ability to harbor l. amazonensis. laboratory-reared lu. cru ... | 2017 | 28234913 |
| mitochondrial proteomics of antimony and miltefosine resistant leishmania infantum. | antimony (sbiii) and miltefosine (mil) are important drugs for the treatment of leishmania parasite infections. the mitochondrion is likely to play a central role in sbiii and mil induced cell death in this parasite. enriched mitochondrial samples from leishmania promastigotes selected step by step for in vitro resistance to sbiii and mil were subjected to differential proteomic analysis. a shared decrease in both mutants in the levels of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase, a ... | 2015 | 28248274 |
| homeopathic medicines cause th1 predominance and induce spleen and megakaryocytes changes in balb/c mice infected with leishmania infantum. | the prevalence of th1/th2 response, spleen changes and megakaryocytes were investigated in balb/c mice (n=138) infected with leishmania infantum, and treated with leishmania infantum 30× (10(-30)) biotherapy - bioli30×. we performed controlled experiments using 8-to-12-week-old mice, infected with 5×10(7)l. infantum promastigotes, divided into eight groups: g1 (healthy), g2 (infected with l. infantum), g3 (bioli30× pre-treated), g4 (bioli30× pre/post-treated), g5 (bioli30× post-treated), g6 (wat ... | 2017 | 28254560 |
| antiprotozoal activity of triazole derivatives of dehydroabietic acid and oleanolic acid. | tropical parasitic diseases such as chagas disease and leishmaniasis are considered a major public health problem affecting hundreds of millions of people worldwide. as the drugs currently used to treat these diseases have several disadvantages and side effects, there is an urgent need for new drugs with better selectivity and less toxicity. structural modifications of naturally occurring and synthetic compounds using click chemistry have enabled access to derivatives with promising antiparasiti ... | 2017 | 28264505 |
| neurological manifestations in dogs naturally infected by leishmania infantum: descriptions of 10 cases and a review of the literature. | in order to evaluate possible nervous system involvement in canine leishmaniasis, retrospective evaluation of all medical records of leishmaniotic dogs exhibiting neurological signs referred to our hospital over a 5-year period was performed. the records of 10 dogs were reviewed. depending on the neuroanatomical localisation, the dogs underwent advanced diagnostic imaging, cerebrospinal fluid analysis, electrodiagnostic testing and histopathologic evaluations. the final neurological diagnosis wa ... | 2017 | 28267216 |
| natural infection of nesokia indica with leishmania major and leishmania infantum parasites in damghan city, northern iran. | various species of rodents are proven reservoir hosts of zoonotic cutaneous leishmaniasis in different provinces of iran and potential reservoir hosts of zoonotic visceral leishmaniasis. therefore, this study was conducted to determine the leishmanial infection of rodents in damghan city from april to september, 2015. sum of 100 rodents of three species; nesokia indica (95), mus musculus (3), and microtus socialis (2), were trapped alive and their tissue samples were examined using parasitologic ... | 2017 | 28267441 |
| structure of salo, a leishmaniasis vaccine candidate from the sand fly lutzomyia longipalpis. | immunity to the sand fly salivary protein salo (salivary anticomplement of lutzomyia longipalpis) protected hamsters against leishmania infantum and l. braziliensis infection and, more recently, a vaccine combination of a genetically modified leishmania with salo conferred strong protection against l. donovani infection. because of the importance of salo as a potential component of a leishmaniasis vaccine, a plan to produce this recombinant protein for future scale manufacturing as well as knowl ... | 2017 | 28278244 |
| toll-like receptor 2 is required for inflammatory process development during leishmania infantum infection. | visceral leishmaniasis (vl) is a chronic and fatal disease caused by leishmania infantum in brazil. leukocyte recruitment to infected tissue is a crucial event for the control of infections such as vl. among inflammatory cells, neutrophils are recruited to the site of leishmania infection, and these cells may control parasite replication through oxidative or non-oxidative mechanisms. the recruitment, activation and functions of the neutrophils are coordinated by pro-inflammatory cytokines and ch ... | 2017 | 28280488 |
| chroman-4-one derivatives targeting pteridine reductase 1 and showing anti-parasitic activity. | flavonoids have previously been identified as antiparasitic agents and pteridine reductase 1 (ptr1) inhibitors. herein, we focus our attention on the chroman-4-one scaffold. three chroman-4-one analogues (1-3) of previously published chromen-4-one derivatives were synthesized and biologically evaluated against parasitic enzymes (trypanosoma brucei ptr1-tbptr1 and leishmania major-lmptr1) and parasites (trypanosoma brucei and leishmania infantum). a crystal structure of tbptr1 in complex with com ... | 2017 | 28282886 |
| comparison of myocardial damage among dogs at different stages of clinical leishmaniasis and dogs with idiopathic chronic kidney disease. | canine leishmaniasis (canl) is a systemic disease caused by the protozoan parasite leishmania infantum. myocarditis in canl has been described previously in canl by histopathological analysis of post-mortem specimens and by evaluation of cardiac troponin i (ctni) levels. however, the degree of myocardial damage at different stages of canl and the role that concurrent azotaemia plays in this myocardial injury are unknown. the aim of this study was to prospectively evaluate and compare the presenc ... | 2017 | 28283071 |
| molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease. | the spleen is a highly perfused organ involved in the immunological control and elimination of vector-borne pathogens (vbp), which could have a fundamental role in the pathogenesis of splenic disease. this study aimed to evaluate certain vbp in samples from dogs with splenic lesions. | 2017 | 28285583 |
| serological association between leishmania infantum and sand fly fever sicilian (but not toscana) virus in sheltered dogs from southern portugal. | phlebotomine sand fly-borne diseases such as leishmanioses and phleboviruses are emerging threats to animal and public health. canine leishmaniosis caused by leishmania infantum is an endemic zoonosis in portugal. antibodies to toscana virus (tosv) and sand fly fever sicilian virus (sfsv) were also reported in dogs from the south of the country. the aim of this work was to evaluate a possible association between exposure to l. infantum, tosv and sfsv in sheltered dogs from the south of portugal. | 2017 | 28285587 |
| evaluation of four molecular methods to detect leishmania infection in dogs. | canine leishmaniasis, a zoonotic disease caused by leishmania infantum vectored by phlebotomine sand flies, is considered a relevant veterinary and public health problem in various countries, namely in the mediterranean basin and brazil, where dogs are considered the main reservoir hosts. not only diseased dogs but also those subclinically infected play a relevant role in the transmission of l. infantum to vectors; therefore, early diagnosis is essential, under both a clinical and an epidemiolog ... | 2017 | 28285595 |
| prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from cyprus. | feline infectious agent studies are lacking in cyprus. the aims of this study were to determine the prevalence and risk factors for various feline infectious agents, including feline vector-borne pathogens (fvbp), in cats from cyprus. | 2017 | 28285597 |
| diagnostic performance of elisa, ifat and western blot for the detection of anti-leishmania infantum antibodies in cats using a bayesian analysis without a gold standard. | anti-leishmania antibodies are increasingly investigated in cats for epidemiological studies or for the diagnosis of clinical feline leishmaniosis. the immunofluorescent antibody test (ifat), the enzyme-linked immunosorbent assay (elisa) and western blot (wb) are the serological tests more frequently used. the aim of the present study was to assess diagnostic performance of ifat, elisa and wb to detect anti-l. infantum antibodies in feline serum samples obtained from endemic (n = 76) and non-end ... | 2017 | 28285598 |
| canine leishmaniosis caused by leishmania major and leishmania tropica: comparative findings and serology. | infection and clinical disease associated with leishmania major and leishmania tropica, two common agents of human cutaneous leishmaniosis, have rarely been reported in dogs. this study describes dogs infected with these leishmania spp. prevalent in the middle east and north africa, and compares the serological response of dogs infected with leishmania infantum, l. major or l. tropica to whole promastigote antigen enzyme-linked immunosorbent assay (elisa) of each species and to rk39 dipstick. | 2017 | 28285601 |
| histological and parasitological distinctive findings in clinically-lesioned and normal-looking skin of dogs with different clinical stages of leishmaniosis. | normal-looking skin of dogs with leishmaniosis frequently shows microscopic lesions along with the presence of leishmania amastigotes. however, histological lesions with or without detection of amastigotes might not occur in less severe clinical cases. in addition, comparative studies between paired clinically-lesioned and normal-looking skin samples from dogs with different disease severity are lacking. the objective of this study was to compare histological and parasitological findings by leis ... | 2017 | 28288670 |
| the inflammatory cytokine effect of pam3csk4 tlr2 agonist alone or in combination with leishmania infantum antigen on ex-vivo whole blood from sick and resistant dogs. | a wide spectrum of clinical manifestations and immune responses exist in canine l. infantum infection. ibizan hounds are more "resistant" to disease than other dog breeds. recognition of pathogen-associated molecule patterns by toll like receptors (tlrs) rapidly triggers a variety of anti-microbial immune responses through the induction of pro-inflammatory cytokines such as tnf-α and il-6 which may play an important role in controlling leishmania infection. the main objective of this study was t ... | 2017 | 28288677 |
| feline and canine leishmaniosis and other vector-borne diseases in the aeolian islands: pathogen and vector circulation in a confined environment. | vector-borne diseases (vbds) are prevalently investigated in dogs. studies on feline vbds are scant, though feline leishmaniosis (fel) is increasingly recognised as a disease of cats in endemic areas. comprehensive investigations on the distribution of vbds in populations of cats and dogs living in relatively small geographical areas, such as islands, are currently lacking. in this study the prevalence of leishmania infantum and other vbd pathogens was assessed in cohorts of cats and dogs living ... | 2017 | 28288759 |
| probing the efficacy of a heterologous leishmania/l. viannia braziliensis recombinant enolase as a candidate vaccine to restrict the development of l. infantum in balb/c mice. | in the present study, the leishmania braziliensis enolase protein was evaluated as a vaccine candidate against visceral leishmaniasis (vl). the dna sequence was cloned and the recombinant protein (renolase) was evaluated as a vaccine, associated with saponin, as an immune adjuvant. the protective efficacy of the renolase plus saponin combination was investigated in balb/c mice against leishmania infantum infection. the results revealed that the vaccine induced higher levels of ifn-γ, il-12, and ... | 2017 | 28288798 |
| new hydrazine and hydrazide quinoxaline 1,4-di-n-oxide derivatives: in silico admet, antiplasmodial and antileishmanial activity. | we report the design (in silico admet criteria), synthesis, cytotoxicity studies (hepg-2 cells), and biological evaluation of 15 hydrazine/hydrazide quinoxaline 1,4-di-n-oxide derivatives against the 3d7 chloroquine sensitive strain and fcr-3 multidrug resistant strain of plasmodium falciparum and leishmania infantum (axenic amastigotes). fourteen of derivatives are novel quinoxaline 1,4-di-n-oxide derivatives. compounds 18 (3d7 ic50=1.40μm, fcr-3 ic50=2.56μm) and 19 (3d7 ic50=0.24μm, fcr-3 ic50 ... | 2017 | 28291694 |
| distinct leishmania infantum strains circulate in humans and dogs in the emilia-romagna region, northeastern italy. | human leishmaniasis is an emerging problem in italy and is on the increase in the emilia-romagna region, northeastern part of the country. nevertheless, studies dealing with the molecular characterization of leishmania spp. circulating in these areas are limited. in the present work, we explored the genetic polymorphism of leishmania isolates from 28 cases of canine leishmaniasis and three cases of human visceral leishmaniasis (vl), which occurred in 2013-2014 in the emilia-romagna region. the c ... | 2017 | 28301296 |
| molecular characterization of leishmania parasites in giemsa-stained slides from cases of human cutaneous and visceral leishmaniasis, eastern algeria. | in algeria, visceral leishmaniasis (vl) is due to leishmania (l.) infantum, while three cutaneous forms (cl) are caused by leishmania major, leishmania tropica and leishmania infantum. in this study, the use of giemsa-stained slides was evaluated with two pcr techniques, in eastern algeria. | 2017 | 28301305 |
| insight into the mechanism of action of temporin-sha, a new broad-spectrum antiparasitic and antibacterial agent. | antimicrobial peptides (amps) are promising drugs to kill resistant pathogens. in contrast to bacteria, protozoan parasites, such as leishmania, were little studied. therefore, the antiparasitic mechanism of amps is still unclear. in this study, we sought to get further insight into this mechanism by focusing our attention on temporin-sha (sha), a small broad-spectrum amp previously shown to be active against leishmania infantum. to improve activity, we designed analogs of sha and compared the a ... | 2017 | 28319176 |
| a library of seleno-compounds and leishmania species: rising evidences towards novel agents. | in vitro leishmanicidal activity of a series of forty-eight selenium derivatives, recently synthesized, was tested on leishmania infantum and leishmania braziliensis parasites, using promastigotes and intracellular amastigote forms. the cytotoxicity of the tested compounds on j774.2 macrophage cells was also measured in order to establish their selectivity. six of the tested compounds (8, 10, 11, 15, 45 and 48) showed selectivity indexes higher than those of the reference drug glucantime for bot ... | 2017 | 28320721 |
| glucantime® causes oxidative stress-derived dna damage in balb/c mice infected by leishmania (leishmania) infantum. | leishmaniasis is a neglected tropical disease caused by over 20 species of the protozoan parasite leishmania regarding treatment, glucantime® is the first-choice drug recommended by the world health organization for the treatment of all types of leishmaniasis. however, the mechanisms of action and toxicity of pentavalent antimonials, including genotoxic effetcs, remain unclear. therefore, the mechanism by which glucantime® causes dna damage was investigated in balb/c mice infected by leishmania ... | 2017 | 28320726 |
| a vaccine therapy for canine visceral leishmaniasis promoted significant improvement of clinical and immune status with reduction in parasite burden. | herein, we evaluated the treatment strategy employing a therapeutic heterologous vaccine composed of antigens of leishmania braziliensis associated with mpl adjuvant (lbmpl vaccine) for visceral leishmaniasis (vl) in symptomatic dogs naturally infected by leishmania infantum. sixteen dogs received immunotherapy with mpl adjuvant (n = 6) or with a vaccine composed of antigens of l. braziliensis associated with mpl (lbmpl vaccine therapy, n = 10). dogs were submitted to an immunotherapeutic scheme ... | 2017 | 28321217 |
| leishmania donovani nucleoside hydrolase (nh36) domains induce t-cell cytokine responses in human visceral leishmaniasis. | development of immunoprotection against visceral leishmaniasis (vl) focused on the identification of antigens capable of inducing a th1 immune response. alternatively, antigens targeting the cd8 and t-regulatory responses are also relevant in vl pathogenesis and worthy of being included in a preventive human vaccine. we assessed in active and cured patients and vl asymptomatic subjects the clinical signs and cytokine responses to the leishmania donovani nucleoside hydrolase nh36 antigen and its ... | 2017 | 28321221 |
| an in silico functional annotation and screening of potential drug targets derived from leishmania spp. hypothetical proteins identified by immunoproteomics. | leishmaniasis is a parasitic disease caused by the protozoan of the leishmania genus. while no human vaccine is available, drugs such as pentavalent antimonials, pentamidine and amphotericin b are used for treat the patients. however, the high toxicity of these pharmaceutics, the emergence of parasite resistance and/or their high cost have showed to the urgent need of identify new targets to be employed in the improvement of the treatment against leishmaniasis. in a recent immunoproteomics appro ... | 2017 | 28327439 |
| seasonality of sand flies (diptera: psychodidae) and leishmania dna detection in vector species in an area with endemic visceral leishmaniasis. | leishmaniases are a serious health problem in southeast brazil, including the city of belo horizonte (bh), minas gerais state (mg), where there are high rates of incidence and mortality due to visceral leishmaniases. bh is divided into nine sanitary districts (sd) of which one, the venda nova sd, was selected for this study because it has high rates of positivity for canine leishmaniasis and high incidence of human leishmaniasis. | 2017 | 28327794 |
| serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in indigenous reserves in the brazilian amazon region. | leishmaniasis is endemic to the northern, northeastern, central-western, and southeastern regions of brazil. we aimed to assess the epidemiological situation of leishmaniasis in humans and dogs in indigenous villages located in the states of mato grosso and tocantins using a serological survey conducted in may 2011. | 2017 | 28327803 |
| (r)-(-)-aloesaponol iii 8-methyl ether from eremurus persicus: a novel compound against leishmaniosis. | leishmaniosis is a neglected tropical disease which affects several millions of people worldwide. the current drug therapies are expensive and often lack efficacy, mainly due to the development of parasite resistance. hence, there is an urgent need for new drugs effective against leishmania infections. as a part of our ongoing study on the phytochemical characterization and biological investigation of plants used in the traditional medicine of western and central asia, in the present study, we f ... | 2017 | 28338625 |
| retrospective pcr-based species identification of leishmania in two patients with visceral leishmaniasis in serbia. | retrospective molecular identification of leishmania parasites in two patients with visceral leishmaniasis (vl) previously treated in serbia was carried out. dna was isolated from unstained bone marrow smears (bmss) kept for 11 and 8 years. genus-specific real-time pcr was combined with conventional pcr and sequencing for detection and species identification. | 2016 | 28348785 |
| feline visceral leishmaniasis in kerman, southeast of iran: serological and molecular study. | visceral leishmaniasis (vl) is a fatal zoonotic disease in tropical and sub-tropical countries including iran. dogs constitute the main domestic reservoir for vl (kala-azar) in iran but incidence of the disease in cats from fars and east azerbaijan provinces has led to propose them as secondary reservoirs, and possible expansion of the feline role in the transmission of disease. the aim of this study was to evaluate the prevalence of leishmania infantum infection in stray cats in kerman city by ... | 2017 | 28352052 |
| an integrated overview of the midgut bacterial flora composition of phlebotomus perniciosus, a vector of zoonotic visceral leishmaniasis in the western mediterranean basin. | the leishmania developmental life cycle within its sand fly vector occurs exclusively in the lumen of the insect's digestive tract in the presence of symbiotic bacteria. the composition of the gut microbiota and the factors that influence its composition are currently poorly understood. a set of factors, including the host and its environment, may influence this composition. it has been demonstrated that the insect gut microbiota influences the development of several human pathogens, such as pla ... | 2017 | 28355207 |
| detection of high leishmania infantum loads in phlebotomus perniciosus captured in the leishmaniasis focus of southwestern madrid region (spain) by real time pcr. | since 2010 a human leishmaniasis outbreak has been notified in southwestern madrid region that still remains active. entomological surveys have been carried out in the affected area in order to obtain information about species diversity, distribution, and density of sand flies. moreover, molecular identification of blood meal preferences of sand flies and molecular detection of leishmania infantum has been performed. in this work, we optimized a real time pcr assay in order to determine parasite ... | 2017 | 28356232 |
| simple dialkyl pyrazole-3,5-dicarboxylates show in vitro and in vivo activity against disease-causing trypanosomatids. | the synthesis and antiprotozoal activity of some simple dialkyl pyrazole-3,5-dicarboxylates (compounds 2-6) and their sodium salts (pyrazolates) (compounds 7-9) against trypanosoma cruzi, leishmania infantum and leishmania braziliensis are reported. in most cases the studied compounds showed, especially against the clinically significant amastigote forms, in vitro activities higher than those of the reference drugs (benznidazole for t. cruzi and glucantime for leishmania spp.); furthermore, the ... | 2017 | 28367781 |
| composition and leishmanicidal activity of the essential oil of vernonia polyanthes less (asteraceae). | the composition of the essential oil (eo) from leaves of vernonia polyanthes and the evaluation of its leishmanicidal potential are reported here for the first time. the oil obtained by hydrodistillation was analysed by combination of gc and gc/ms. thirty-five compounds were identified, representing 91.8% of the oil composition. the oil consists primarily of monoterpenes (37.1%), sesquiterpenes (26.3%) and oxygenated sesquiterpenes (23.9%), myrcene (34.3%), zerumbone (15.8%), bicyclogermacrene ( ... | 2017 | 28368666 |
| comparison of recombinant proteins of kinesin 39, heat shock protein 70, heat shock protein 83, and glycoprotein 63 for antibody detection of leishmania martiniquensis infection. | leishmania martiniquensis, a zoonotic hemoflagellate, is a causative agent of cutaneous (cl) and visceral leishmaniasis (vl) among humans and animals. this organism, first reported in martinique island, now has become an emerging infectious agent in thailand. symptomatic cases of l. martiniquensis infection among humans have continuously increased. in the meantime, asymptomatic infection of this novel species has seriously created national public health awareness and concern to prevent and contr ... | 2017 | 28370779 |
| new alkenyl derivative from piper malacophyllum and analogues: antiparasitic activity against trypanosoma cruzi and leishmania infantum. | alkylphenols isolated from piper malacophyllum (piperaceae), gibbilimbols a and b, showed interesting activity against the parasites trypanosoma cruzi and leishmania infantum. in continuation to our previous work, a new natural product from the essential oil of the leaves of p. malacophyllum was isolated, the 5-[(3e)-oct-3-en-1-il]-1,3-benzodioxole, and also a new set of five compounds was prepared. the antiparasitic activity of the natural product was evaluated in vitro against these parasites, ... | 2017 | 28371557 |
| causes and consequences of the leishmania infantum burden in patients with kala-azar -ndash- a study of 625 patients. | an infected host's leishmania infantum load in blood is considered to be an estimate of his or her total parasite burden. therefore, the measurement of blood parasite burden is important in the identification of factors involved in parasite control. | 2017 | 28380279 |
| anion exchange through band 3 protein in canine leishmaniasis at different stages of disease. | band 3 protein efficiency in mediating cl(-)/hco3(-) exchange through erythrocytes membrane is reduced by oxidative stress. the aim of the present study was to verify whether and how anion transport through band 3 protein may be useful in monitoring canine leishmaniasis (leishmania infantum) development, a disease associated to membrane protein degradation and oxidative stress. to accomplish this aim, serological analysis to determine ifat (immunofluorescence antibody test) titers against leishm ... | 2017 | 28382481 |
| in vitro antileishmanial activity of fisetin flavonoid via inhibition of glutathione biosynthesis and arginase activity in leishmania infantum. | with the increasing emergence of drug resistant leishmania sp. in recent years, combination therapy has been considered as a useful way to treat and control of leishmaniasis. the present study was designed to evaluate the antileishmanial effects of the fisetin alone and combination of fisetin plus meglumine antimoniate (fi-ma) against leishmania infantum. the ic50 values for fisetin were obtained 0.283 and 0.102 μm against promastigotes and amastigote forms, respectively. meglumine antimoniate ( ... | 2017 | 28385129 |
| performance of leishmania braziliensis enolase protein for the serodiagnosis of canine and human visceral leishmaniosis. | in the present study, leishmania braziliensis enolase was cloned and the recombinant protein (renolase) was evaluated for the serodiagnosis of canine and human visceral leishmaniosis (vl). for the canine vl diagnosis, this study examined serum samples of leishmania infantum-infected dogs, from non-infected animals living in endemic or non-endemic areas of leishmaniosis, as well as those from leish-tec(®)-vaccinated dogs and trypanosoma cruzi or ehrlichia canis experimentally infected animals. fo ... | 2017 | 28385540 |
| revealing a novel otubain-like enzyme from leishmania infantum with deubiquitinating activity toward k48-linked substrate. | deubiquitinating enzymes (dubs) play an important role in regulating a variety of eukaryotic processes. in this context, exploring the role of deubiquitination in leishmania infantum could be a promising alternative to search new therapeutic targets for leishmaniasis. here we present the first characterization of a dub from l. infantum, otubain (otuli), and its localization within parasite. the recombinant otuli (rotuli) showed improved activity on lysine 48 (k48)-linked over k63-linked tetra-ub ... | 2017 | 28386537 |
| bmajpla2-ii, a basic lys49-phospholipase a2 homologue from bothrops marajoensis snake venom with parasiticidal potential. | snake venoms contain various proteins, especially phospholipases a2 (pla2s), which present potential applications in diverse areas of health and medicine. in this study, a new basic pla2 from bothrops marajoensis with parasiticidal activity was purified and characterized biochemically and biologically. b. marajoensis venom was fractionated through cation exchange followed by reverse phase chromatographies. the isolated toxin, bmajpla2-ii, was structurally characterized with maldi-tof (matrix-ass ... | 2017 | 28390830 |
| protein malnutrition promotes dysregulation of molecules involved in t cell migration in the thymus of mice infected with leishmania infantum. | protein malnutrition, the most deleterious cause of malnutrition in developing countries, has been considered a primary risk factor for the development of clinical visceral leishmaniasis (vl). protein malnutrition and infection with leishmania infantum leads to lymphoid tissue disorganization, including changes in cellularity and lymphocyte subpopulations in the thymus and spleen. here we report that protein malnutrition modifies thymic chemotactic factors by diminishing the ccl5, cxcl12, igf1, ... | 2017 | 28397794 |
| eurasian golden jackal as host of canine vector-borne protists. | jackals are medium-sized canids from the wolf-like clade, exhibiting a unique combination of ancestral morphotypes, broad trophic niches, and close phylogenetic relationships with the wolf and dog. thus, they represent a potential host of several pathogens with diverse transmission routes. recently, populations of the eurasian golden jackal canis aureus have expanded into the western palaearctic, including most of europe. the aim of our study was to examine eurasian golden jackals from romania, ... | 2017 | 28410591 |
| occurrence of leishmania infantum in the central nervous system of naturally infected dogs: parasite load, viability, co-infections and histological alterations. | zoonotic visceral leishmaniasis is caused by the protozoan leishmania infantum and little is known about the occurrence and pathogenesis of this parasite in the cns. the aims of this study were to evaluate the occurrence, viability and load of l. infantum in the cns, and to identify the neurological histological alterations associated with this protozoan and its co-infections in naturally infected dogs. forty-eight leishmania-seropositive dogs from which l. infantum was isolated after necropsy w ... | 2017 | 28419136 |
| what pre-columbian mummies could teach us about south american leishmaniases? | a recent report on the taxonomic profile of the human gut microbiome in pre-columbian mummies (santiago-rodriguez et al. 2016) gives for the first time evidence of the presence of leishmania dna (sequences similar to leishmania donovani according to the authors) that can be reminiscent of visceral leishmaniasis during the pre-columbian era. it is commonly assumed that leishmania infantum, the etiological agent of american visceral leishmaniasis (avl) was introduced into the new world by the iber ... | 2017 | 28423167 |
| a non-pathogenic recombinant leishmania expressing lipophosphoglycan 3 against experimental infection with leishmania infantum. | visceral leishmaniasis (vl) is caused by leishmania infantum in the mediterranean basin and affects primarily children and immunosuppressed individuals. various strategies of vaccination have so far been examined by either protein or dna without achievable complete protection against the disease. the live non-pathogenic lizard parasite, leishmania tarentolae, expressing elected leishmania antigens has recently provided a promising new approach as a safe and effective live vaccine candidate to pr ... | 2017 | 28426153 |
| canine β-defensin-1 (cbd1) gene as a possible marker for leishmania infantum infection in dogs. | canine leishmaniasis caused by leishmania infantum is a parasitic disease of great veterinary significance. some dogs infected by l. infantum may mount a strong cellular immune response and clear the infection, while others may respond with exaggerated antibody production against the parasite and develop an overt disease, which may be fatal, if left untreated. the initial factors triggering the polarization of the immune response towards a predominantly t-helper 1 or t-helper 2 cytokines, as wel ... | 2017 | 28427438 |
| t1/st2 deficient mice display protection against leishmania infantum experimental infection. | t1/st2 is a surface marker selectively expressed on type 2 helper (th2) effector cells. as leishmania infection in susceptible balb/c mice have ascribed to a polarized th2 response, this study aim to investigate the t1/st2 (the receptor for il-33), as a typical th2 marker in the postulation that a shift towards a beneficial th1 response would occur in the absence of st2. for this, st2 knockout (st2(-/-)) and wt balb/c mice were experimentally infected in the retro-orbital sinus with l. infantum. ... | 2017 | 28427965 |
| production of a conjugate between the rk346 antigen from leishmania infantum and the horseradish peroxidase c for the detection of rk346 antibodies. | it was designed and characterized a reporter system to be captured by an- tibodies bound to elisa plates. the system was designed with the rk346 from leishmania infantum, a highly antigenic and specific protein. the rk346 was coupled to the horseradish peroxidase c (hrpc) from armoracia rusticana using glutaraldehyde or sulfo-smcc. gluta- raldehyde conjugation was performed in two steps. separation of conjugates was carried out using a sepharose s-200 in size exclusion chromatography (sec); frac ... | 2016 | 28429896 |
| first example of peptides targeting the dimer interface of leishmania infantum trypanothione reductase with potent in vitro antileishmanial activity(). | a series of 9-mer and 13-mer amide-bridged cyclic peptides derived from the linear prototype ac-pkiiqsvgis-nle-k-nle-nh2 (toro et al. chembiochem2013) has been designed and synthesized by introduction of the lactam between amino acid side chains that are separated by one helical turn (i, i+4). all of these compounds were tested in vitro as both dimerization and enzyme inhibitors of leishmania infantum trypanothione reductase (li-tryr). three of the 13-mer cyclic peptide derivatives (3, 4 and 6) ... | 2017 | 28431354 |
| computer-aided discovery of two novel chalcone-like compounds active and selective against leishmania infantum. | leishmaniasis are infectious diseases caused by parasites of genus leishmania that affect affects 12 million people in 98 countries mainly in africa, asia, and latin america. effective treatments for this disease are urgently needed. in this study, we present a computer-aided approach to investigate a set of 32 recently synthesized chalcone and chalcone-like compounds to act as antileishmanial agents. as a result, nine most promising compounds and three potentially inactive compounds were experi ... | 2017 | 28434763 |
| detection of leishmania infantum dna in conjunctival swabs of cats by quantitative real-time pcr. | although some studies have investigated the potential role of cats as a reservoir for leishmania, their role in the epidemiology of visceral leishmaniasis (vl) is still poorly understood. molecular diagnostic techniques are an important tool in vl diagnosis, and pcr shows high sensitivity and specificity for leishmania spp. detection. quantitative real-time pcr (qpcr) is a method that permits quantitative analysis of a large number of samples, resulting in more sensitive, accurate, and reproduci ... | 2017 | 28438522 |
| investigation of the anti-leishmania (leishmania) infantum activity of some natural sesquiterpene lactones. | leishmaniases are neglected infectious diseases caused by parasites of the 'protozoan' genus leishmania. depending on the parasite species, different clinical forms are known as cutaneous, muco-cutaneous, and the visceral leishmaniasis (vl). vl is particularly fatal and the therapy presents limitations. in the search for new anti-leishmanial hit compounds, seven natural sesquiterpene lactones were evaluated against promastigotes and intracellular amastigotes of leishmania (leishmania) infantum, ... | 2017 | 28441357 |
| epidemiological role of dogs since the human leishmaniosis outbreak in madrid. | canine leishmaniosis (canl) has been in the spotlight since the 2009 outbreak of human leishmaniosis in madrid. in the framework of the leishmaniosis surveillance programme set up in madrid, this study examines leishmania-specific seroprevalences in stray dogs for the outbreak area and rest of the madrid region over the period spanning from the outbreak to the present (2009-2016). these data are of interest because stray dogs could be sentinels for disease surveillance in endemic areas. since 20 ... | 2017 | 28446214 |
| suspected cutaneous leishmaniasis in a sample of westerns sicily residents: what correlation with occupation? | leishmaniasis is a widespread infectious disease, but there is not much information about its prevalence in high risk occupational categories. | 2017 | 28446739 |
| prevalence and geographic distribution of vector-borne pathogens in apparently healthy dogs in croatia. | vector-borne pathogens (vbps) are a group of globally extended and quickly spreading pathogens that are transmitted by various arthropod vectors. the aim of the present study was to investigate the seroprevalence against babesia canis, anaplasma phagocytophilum, borrelia burgdorferi sensu lato, leishmania infantum, dirofilaria immitis, and ehrlichia canis in dogs in croatia. we investigated 435 randomly selected apparently healthy dogs in 13 different locations of croatia for antibodies to b. ca ... | 2017 | 28448211 |
| [urban outbreak of visceral leishmaniasis in neiva (colombia)]. | objective characterize the foci of visceral leishmaniasis infection in neiva with a clinical and epidemiological approach. materials and methods six children consulted medical services with hepatosplenomegaly. they were found to have anemia and leukopenia. the diagnosis was performed by bone marrow (five patients) and spleen (1 patient) aspiration. an active search for cases was carried out in the community. anti-leishmania infantum antibodies were also sought out using indirect immunofluorescen ... | 2015 | 28453072 |
| first record of lutzomyia (lutzomyia) longipalpis (diptera: psychodidae: phlebotominae) on the trinational frontier (brazil-peru-bolivia) of south-western amazonia. | in south america, the main sand fly species involved in the transmission of leishmania infantum chagasi (cunha & chagas, 1937), etiological agent of the visceral leishmaniasis (vl), is lutzomyia longipalpis (lutz & neiva, 1912). the species has been recorded in colombia, venezuela, bolivia, argentina, paraguay, uruguay, and brazil, where it is recorded in 24 of the 27 brazilian states, except acre, amazonas, and santa catarina. collections were carried out for one year (april 2013 to march 2014) ... | 2017 | 28453779 |
| 7-substituted 2-nitro-5,6-dihydroimidazo[2,1-b][1,3]oxazines: novel antitubercular agents lead to a new preclinical candidate for visceral leishmaniasis. | within a backup program for the clinical investigational agent pretomanid (pa-824), scaffold hopping from delamanid inspired the discovery of a novel class of potent antitubercular agents that unexpectedly possessed notable utility against the kinetoplastid disease visceral leishmaniasis (vl). following the identification of delamanid analogue dndi-vl-2098 as a vl preclinical candidate, this structurally related 7-substituted 2-nitro-5,6-dihydroimidazo[2,1-b][1,3]oxazine class was further explor ... | 2017 | 28459575 |
| pediatric visceral leishmaniasis caused by leishmania infantum in northern cyprus. | 2017 | 28471743 | |
| occurrence and probability maps of lutzomyia longipalpis and lutzomyia cruzi (diptera: psychodidae: phlebotominae) in brazil. | leishmaniases are serious diseases caused by trypanosomatid protozoans of the genus leishmania transmitted by the bite of phlebotomine sand flies. we analyzed records pertaining to lutzomyia longipalpis (lutz and neiva, 1912) and lutzomyia cruzi (mangabeira, 1938) in brazil from the following sources: the collection of phlebotomine sand flies of the centro de pesquisas rené rachou/fiocruz (fiocruz-colfleb), the "specieslink" (cria) database, from systematic surveys of scientific articles and gra ... | 2017 | 28472338 |
| in vitro inhibition of leishmania attachment to sandfly midguts and ll-5 cells by divalent metal chelators, anti-gp63 and phosphoglycans. | leishmania braziliensis and leishmania infantum are the causative agents of cutaneous and visceral leishmaniasis, respectively. several aspects of the vector-parasite interaction involving gp63 and phosphoglycans have been individually assayed in different studies. however, their role under the same experimental conditions was not studied yet. here, the roles of divalent metal chelators, anti-gp63 antibodies and purified type i phosphoglycans (pgs) were evaluated during in vitro parasite attachm ... | 2017 | 28472733 |
| leishmania infantum dna detected in phlebotomine species from puerto iguazú city, misiones province, argentina. | in puerto iguazú city, argentina, human and canine visceral leishmaniasis cases have been recorded since the year 2010, with leishmania infantum as the etiological agent and lutzomyia longipalpis as its main vector. in the present study, polymerase chain reaction and sequencing were used to detect l. infantum dna in 3.9% of the female sandflies captured in puerto iguazú city. this is the first report of l. infantum dna detection in micropygomyia quinquefer, and the second one in lu. longipalpis ... | 2017 | 28476601 |
| in vitro evaluation of cytotoxicity and leishmanicidal activity of phthalimido-thiazole derivatives. | it is estimated that the worldwide prevalence of leishmaniasis is around 12 million individuals in 80 countries, with 400,000new cases per year. in the search for new leishmanicidal agents, the hybrid phthalimido-thiazoles have been identified as an important scaffold for drug design and discovery. the present study thus reports the in vitro activity of a series of phthalimido-thiazole derivatives. cytotoxicity against a strain of l. infantum, vero cells, j774 macrophages and peritoneal macropha ... | 2017 | 28478133 |
| imported leishmaniasis in a dog in a sandfly-populated area in northeastern romania. | we report the importation of a clinical case of canine leishmaniasis (canl) in romania, a country where several types of sandflies are present with the potential to develop a new focus of canl. the staffordshire bull terrier dog was imported into romania from spain 1.5 y before he developed clinical symptomatology that included proliferative dermatitis, lymphadenomegaly, and bilateral uveitis. hematologic analyses showed regenerative anemia and subacute inflammation. cytologic examination of lym ... | 2017 | 28480825 |
| multi-anti-parasitic activity of arylidene ketones and thiazolidene hydrazines against trypanosoma cruzi and leishmania spp. | a series of fifty arylideneketones and thiazolidenehydrazines was evaluated against leishmania infantum and leishmania braziliensis. furthermore, new simplified thiazolidenehydrazine derivatives were evaluated against trypanosoma cruzi. the cytotoxicity of the active compounds on non-infected fibroblasts or macrophages was established in vitro to evaluate the selectivity of their anti-parasitic effects. seven thiazolidenehydrazine derivatives and ten arylideneketones had good activity against th ... | 2017 | 28481276 |
| experimental mixed infection of leishmania (leishmania) amazonensis and leishmania (l.) infantum in hamsters (mesocricetus auratus). | in south america, visceral leishmaniasis is frequently caused by leishmania infantum and, at an unknown frequency, by leishmania amazonensis. therefore, mixed infections with these organisms are possible. mixed infections might affect the clinical course, immune response, diagnosis, treatment and epidemiology of the disease. here we describe the clinical course of mixed infections with l. amazonensis and l. infantum in a hamster model. we show that mixed infections are associated with more sever ... | 2017 | 28487000 |
| strategies for reducing the risk of transfusion-transmitted leishmaniasis in an area endemic for leishmania infantum: a patient- and donor-targeted approach. | in the balearic islands, as in other areas of the mediterranean basin, there is a significant proportion of asymptomatic leishmania (l.) infantum-infected blood donors, who may represent an important threat to transfusion safety. the balearic islands blood bank, located in an area endemic for l. infantum, carried out a study of donors and patients to investigate the impact of this infectious disease on blood safety in the region. | 2017 | 28488962 |