Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
in vitro inhibition of leishmania attachment to sandfly midguts and ll-5 cells by divalent metal chelators, anti-gp63 and phosphoglycans.leishmania braziliensis and leishmania infantum are the causative agents of cutaneous and visceral leishmaniasis, respectively. several aspects of the vector-parasite interaction involving gp63 and phosphoglycans have been individually assayed in different studies. however, their role under the same experimental conditions was not studied yet. here, the roles of divalent metal chelators, anti-gp63 antibodies and purified type i phosphoglycans (pgs) were evaluated during in vitro parasite attachm ...201728472733
first record of lutzomyia (lutzomyia) longipalpis (diptera: psychodidae: phlebotominae) on the trinational frontier (brazil-peru-bolivia) of south-western amazonia.in south america, the main sand fly species involved in the transmission of leishmania infantum chagasi (cunha & chagas, 1937), etiological agent of the visceral leishmaniasis (vl), is lutzomyia longipalpis (lutz & neiva, 1912). the species has been recorded in colombia, venezuela, bolivia, argentina, paraguay, uruguay, and brazil, where it is recorded in 24 of the 27 brazilian states, except acre, amazonas, and santa catarina. collections were carried out for one year (april 2013 to march 2014) ...201728453779
leishmania infantum parasites subvert the host inflammatory response through the adenosine a2a receptor to promote the establishment of infection.adenosine is an endogenously released purine nucleoside that signals through four widely expressed g protein-coupled receptors: a1, a2a, a2b, and a3. of these, a2ar is recognized as mediating major adenosine anti-inflammatory activity. during cutaneous leishmaniasis, adenosine induces immunosuppression, which promotes the establishment of infection. herein, we demonstrated that a2ar signaling is exploited by leishmania infantum parasites, the etiologic agent that causes visceral leishmaniasis, t ...201728775724
in vitro effects of purine and pyrimidine analogues on leishmania donovani and leishmania infantum promastigotes and intracellular amastigotes.inhibition of parasite metabolic pathways is a rationale for new chemotherapeutic strategies. the pyrimidine and purine salvage pathways are thus targets against leishmania donovani and l. infantum, causative agents of visceral human leishmaniasis and canine leishmaniosis. the antiproliferative effect of the pyrimidine analogues cytarabine and 5-fluorouracil and of the purine analogues azathioprine and 6-mercaptopurine was evaluated in vitro on the promastigote and the intracellular amastigote s ...201728682767
leishmaniasis in turkey: visceral and cutaneous leishmaniasis caused by leishmania donovani in turkey.in turkey, the main causative agents are leishmania tropica (l. tropica) and leishmania infantum (l. infantum) for cutaneous leishmaniasis (cl) and l. infantum for visceral leishmaniasis (vl). in this study, we investigated leishmaniasis cases caused by l. donovani and established animal models for understanding its tropism in in vivo conditions. clinical samples (lesion aspirations and bone marrow) obtained from cl/vl patients were investigated using parasitological (smear/nnn) and dna-based te ...201728587839
molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis.infections with parasites of the leishmania donovani complex result in clinical outcomes that range from asymptomatic infection to severe and fatal visceral leishmaniasis (vl). neutrophils are major players of the immune response against leishmania, but their contribution to distinct states of infection is unknown. gene expression data suggest the activation of the netosis pathway during human visceral leishmaniasis. thus, we conducted an exploratory study to evaluate net-related molecules in re ...201728583201
maldi ms imaging investigation of the host response to visceral leishmaniasis.mass spectrometry imaging (msi) of animal tissues has become an important tool for in situ molecular analyses and biomarker studies in several clinical areas, but there are few applications in parasitological studies. leishmaniasis is a neglected tropical disease, and experimental mouse models have been essential to evaluate pathological and immunological processes and to develop diagnostic methods. herein we have employed maldi msi to examine peptides and low molecular weight proteins (2 to 20 ...201728758666
visceral leishmaniasis in southeastern iran: a narrative review.visceral leishmaniasis (vl) has strong links with poverty, substantial medical and veterinary impacts. this review aimed to focus in studies published during 1994-2016 on vl in southeastern iran.201728761456
protection mediated by chemokine cxcl10 in balb/c mice infected by leishmania infantum.visceral leishmaniasis (vl) caused by leishmania infantum is characterised by the loss of the ability of the host to generate an effective immune response. chemokines have a direct involvement in the pathogenesis of leishmaniasis, causing a rapid change in the expression of these molecules during infection by leishmania.201728767981
phlebotomine sand fly survey in the focus of leishmaniasis in madrid, spain (2012-2014): seasonal dynamics, leishmania infantum infection rates and blood meal preferences.an unusual increase of human leishmaniasis cases due to leishmania infantum is occurring in an urban area of southwestern madrid, spain, since 2010. entomological surveys have shown that phlebotomus perniciosus is the only potential vector. direct xenodiagnosis in hares (lepus granatensis) and rabbits (oryctolagus cuniculus) collected in the focus area proved that they can transmit parasites to colonized p. perniciosus. isolates were characterized as l. infantum. the aim of the present work was ...201728764772
asymptomatic human blood donors carriers of leishmania infantum: potential reservoirs for visceral leishmaniasis in northwestern iran.little is known regarding transfusion-transmitted leishmaniasis (ttl) and the real global incidence of visceral leishmaniasis (vl) among blood donors as carriers of leishmania spp. in endemic vl foci including iran. recent epidemiological evidences indicate that blood donor may be harbor of leishmania infantum (l. infantum) infection in iran.201728648574
what pre-columbian mummies could teach us about south american leishmaniases?a recent report on the taxonomic profile of the human gut microbiome in pre-columbian mummies (santiago-rodriguez et al. 2016) gives for the first time evidence of the presence of leishmania dna (sequences similar to leishmania donovani according to the authors) that can be reminiscent of visceral leishmaniasis during the pre-columbian era. it is commonly assumed that leishmania infantum, the etiological agent of american visceral leishmaniasis (avl) was introduced into the new world by the iber ...201728423167
multicentre evaluation of a direct agglutination test prototype kit (dat-lpc) for diagnosis of visceral leishmaniasis.in this study, we assessed the sensitivity, specificity, and diagnostic accuracy of a previously developed direct agglutination test (dat) using a freeze-dried antigen derived from leishmania infantum promastigotes and composed in a prototype kit for visceral leishmaniasis (vl) diagnosis, named dat-lpc. to evaluate dat-lpc reproducibility, the kit was used to analyse 207 serum samples from vl patients and 80 serum samples from patients with other parasitic infections or healthy subjects in four ...201728735574
structural and evolutionary analyses of leishmania alba proteins.the alba superfamily proteins share a common rna-binding domain. these proteins participate in a variety of regulatory pathways by controlling developmental gene expression. they also interact with ribosomal subunits, translation factors, and other rna-binding proteins. the leishmania infantum genome encodes two alba-domain proteins, lialba1 and lialba3. in this work, we used homology modeling, protein-protein docking, and molecular dynamics (md) simulations to explore the details of the alba1-a ...201728847609
after infection with leishmania infantum, golden hamsters (mesocricetus auratus) become more attractive to female sand flies (lutzomyia longipalpis).in brazil, human and canine visceral leishmaniasis is caused by infection with leishmania infantum, a protist parasite transmitted by blood-feeding female lutzomyia longipalpis sand flies. the objective of this study was to determine if the odour of hamsters, infected with le. infantum, was more attractive than the odour of the same hamsters, before they were infected. the attractiveness of odour collected from individual hamsters (n = 13), before they were infected, was compared in a longitudin ...201728733676
a poly(lactic-co-glycolic) acid nanovaccine based on chimeric peptides from different leishmania infantum proteins induces dendritic cells maturation and promotes peptide-specific ifnγ-producing cd8(+) t cells essential for the protection against experimental visceral leishmaniasis.visceral leishmaniasis, caused by leishmania (l.) donovani and l. infantum protozoan parasites, can provoke overwhelming and protracted epidemics, with high case-fatality rates. an effective vaccine against the disease must rely on the generation of a strong and long-lasting t cell immunity, mediated by cd4(+) th1 and cd8(+) t cells. multi-epitope peptide-based vaccine development is manifesting as the new era of vaccination strategies against leishmania infection. in this study, we designed chi ...201728659922
an ulcer on the lower lip. 201728648931
environmental factors as key determinants for visceral leishmaniasis in solid organ transplant recipients, madrid, spain.during a visceral leishmaniasis outbreak in an area of madrid, spain, the incidence of disease among solid organ transplant recipients was 10.3% (7/68). being a black person from sub-saharan africa, undergoing transplantation during the outbreak, and residing <1,000 m from the epidemic focus were risk factors for posttransplant visceral leishmaniasis.201728628447
comparative analyses of whole genome sequences of leishmania infantum isolates from humans and dogs in northeastern brazil.the genomic sequences of 20 leishmania infantum isolates collected in northeastern brazil were compared with each other and with the available genomic sequences of 29 l. infantum/donovani isolates from nepal and turkey. the brazilian isolates were obtained in the early 1990s or since 2009 from patients with visceral or non-ulcerating cutaneous leishmaniasis, asymptomatic humans, or dogs with visceral leishmaniasis. two isolates were from the blood and bone marrow of the same visceral leishmanias ...201728606698
application of a specific quantitative real-time pcr (qpcr) to identify leishmania infantum dna in spleen, skin and hair samples of wild leporidae.the aim of this study was to compare a quantitative real-time pcr (qpcr) validated for the detection of leishmania infantum in dogs with a nested pcr but in wild leporidae. additionally, l. infantum results from indirect immunofluorescent antibody test (ifat) and in vitro culture were also compared with qpcr. different samples (spleen, skin and hair) recovered from 224 european rabbits and 70 iberian hares from two green areas of madrid council were analysed for the detection of l. infantum. the ...201728807318
association between mast cells, tissue remodelation and parasite burden in the skin of dogs with visceral leishmaniasis.canine visceral leishmaniosis (cvl) is a zoonosis of major public health impact caused by organisms of the genus leishmania which is transmitted to human and animals by phlebotomine sand flies. the skin is the first point of contact with leishmania parasites for sandy fly vectors and it is considered an important reservoir compartment in infected dogs. the aim of this study was to determine the main histophatologic alterations in ear skin of dogs naturally infected by leishmania infantum with di ...201728807304
comparison of recombinant proteins of kinesin 39, heat shock protein 70, heat shock protein 83, and glycoprotein 63 for antibody detection of leishmania martiniquensis infection.leishmania martiniquensis, a zoonotic hemoflagellate, is a causative agent of cutaneous (cl) and visceral leishmaniasis (vl) among humans and animals. this organism, first reported in martinique island, now has become an emerging infectious agent in thailand. symptomatic cases of l. martiniquensis infection among humans have continuously increased. in the meantime, asymptomatic infection of this novel species has seriously created national public health awareness and concern to prevent and contr ...201728370779
novel areas for prevention and control of canine leishmaniosis.there have been multiple recent advances regarding tools for the control and prevention of canine leishmaniosis (canl), including new preventative vaccines. in this review, these advances are evaluated based on control targets, including vector and parasite. leishvet recommendations are provided for control practices based on the dog's risk of infection. new topical insecticide formulations have proven to be effective in preventing sand fly bites, and subsequently infection. parasite control occ ...201728601528
first molecular detection of leishmania infantum in sergentomyia minuta (diptera, psychodidae) in alentejo, southern portugal.protozoan parasites, such as leishmania spp., are the causative agents of many insect-borne infectious diseases with medical and veterinary importance. leishmaniasis, caused by leishmania spp., is transmitted by female phlebotomine sand flies. in the alentejo region of portugal, located at the north of algarve, cases of human and canine leishmaniasis caused by leishmania infantum have been notified. however, no recent studies regarding the sand fly fauna in the region are available. we therefore ...201728647427
the performance of serological tests for leishmania infantum infection screening in dogs depends on the prevalence of the disease.dogs are considered the main reservoir of leishmania infantum. this protozoan causes visceral leishmaniasis (vl), an uncontrolled urban zoonosis in brazil. serological tests and polymerase chain reaction (pcr) on peripheral blood were performed to identify infected dogs in scenarios of higher and lower prevalence of the disease (teresina and vitória). one-hundred infected and 57 non-infected animals from teresina and 100 non-infected animals from vitória were studied. animal selection was not de ...201728591267
leishmania infection and blood food sources of phlebotomines in an area of brazil endemic for visceral and tegumentary leishmaniasis.the aims of the study were to determine the blood feeding preferences of sandflies and to identify species of leishmania that infected phlebotomines in caxias, maranhão, brazil, an area that is highly endemic for leishmaniasis. sandflies were captured in light traps located in the peridomiciliary environments of randomly selected houses in urban and rural settings between 1800 and 0600 hours on new moon days between march 2013 and february 2015. dna extracts from 982 engorged female sandflies we ...201728837565
spleen damage in a dog naturally infected by leishmania infantum. 201728562772
main lesions in the central nervous system of dogs due to leishmania infantum infection.canine visceral leishmaniasis (cvl) is endemic in são luís maranhão/brazil and it leads a varied clinical picture, including neurological signs.201728821261
leishmania infantum is present in vaginal secretions of naturally infected bitches at lower levels in oestrogenized bitches than in non-oestrogenized bitches.naturally leishmania infantum infected bitches were divided into oestrogenized (n = 11) and non-oestrogenized (n = 6) groups. vaginal secretions were collected for polymerase chain reaction (pcr), and vulval, vaginal and uterine tissues were collected for the immunohistochemical (ihq) identification of l. infantum. parasite dna was identified in vaginal secretions of non-oestrogenized (41.8%) and oestrogenized (18.2%) bitches (p<0.05; fisher's exact test). ihq was positive in vulvar dermis (23.5 ...201728682760
leishmania infantum exoproducts inhibit human invariant nkt cell expansion and activation.leishmania infantum is one of the major parasite species associated with visceral leishmaniasis, a severe form of the disease that can become lethal if untreated. this obligate intracellular parasite has developed diverse strategies to escape the host immune response, such as exoproducts (exo) carrying a wide range of molecules, including parasite virulence factors, which are potentially implicated in early stages of infection. herein, we report that l. infantum exo and its two fractions compose ...201728674535
differential activation of human keratinocytes by leishmania species causing localized or disseminated disease.all leishmania species parasites are introduced into mammalian skin through a sand fly bite, but different species cause distinct clinical outcomes. mouse studies suggest that early responses are critical determinants of subsequent adaptive immunity in leishmaniasis, yet few studies address the role of keratinocytes, the most abundant cell in the epidermis. we hypothesized that leishmania infection causes keratinocytes to produce immunomodulatory factors that influence the outcome of infection. ...201728647347
ecological parameters of the (s)-9-methylgermacrene-b population of the lutzomyia longipalpis complex in a visceral leishmaniasis area in são paulo state, brazil.visceral leishmaniasis (vl) is an important public health challenge in brazil because of the high number of human and canine cases reported annually. leishmania infantum is the etiological agent of vl and lutzomyia longipalpis is its main vector. however, evidence suggests that this taxon constitutes a species complex. in sao paulo state, there are two populations of lu. longipalpis, each secreting distinct pheromones, (s)-9-methylgermacrene-b and cembrene 1; both have been associated with diffe ...201728558760
vaccination with a leishmania infantum hsp70-ii null mutant confers long-term protective immunity against leishmania major infection in two mice models.the immunization with genetically attenuated leishmania cell lines has been associated to the induction of memory and effector t cell responses against leishmania able to control subsequent challenges. a leishmania infantum null mutant for the hsp70-ii genes has been described, possessing a non-virulent phenotype.201728558043
antigenicity of phage clones and their synthetic peptides for the serodiagnosis of canine and human visceral leishmaniasis.in the americas, brazil is responsible by 90% of the cases registered of visceral leishmaniasis (vl), and leishmania infantum is the most common parasite species responsible by disease in brazilian dogs and humans. a precise diagnosis may allow to a faster and more effective treatment against the disease, which increases the possibility of cure, as well as to induce less toxic effects, due to a lower time exposition for the chemotherapeutics. in a previous study, two l. infantum mimotopes, b10 a ...201728629727
new epidemiological pattern of cutaneous leishmaniasis in two pre-saharan arid provinces, southern morocco.three leishmania species are responsible of cutaneous leishmaniasis (cl) in morocco. zoonotic cl due to leishmania major and leishmania infantum, the first is known as established in the eastern arid regions, whereas the latter evolves sporadically, especially in the north. while leishmania tropica, classically considered anthroponotic, is endemic in the semi-arid regions and is largely distributed throughout the country. the aim of this study was to identify the leishmania species causing cl in ...201728527882
insights into the phosphatidylcholine and phosphatidylethanolamine biosynthetic pathways in leishmania parasites and characterization of a choline kinase from leishmania infantum.the protozoan parasite leishmania infantum is a causative agent of the disease visceral leishmaniasis, which can be fatal if not properly treated. phosphatidylcholine (pc) and phosphatidylethanolamine (pe) biosynthesis pathways are attractive targets for new antileishmanial compounds since these leishmania cell membrane phospholipids are important for parasite morphology and physiology. in this work we observed leishmania synthesize pc and pe from extracellular choline and ethanolamine, respecti ...201728754315
in vivo imaging of pathogen homing to the host tissues.hematogenous dissemination followed by tissue tropism is a characteristic of the infectious process of many pathogens including those transmitted by blood-feeding vectors. after entering into the blood circulation, these pathogens must arrest in the target organ before they infect a specific tissue. here, we describe a non-invasive method to visualize and quantify the homing of pathogens to the host tissues. by using in vivo bioluminescence imaging we quantify the accumulation of luciferase-expr ...201728522323
t lymphocytes and macrophages in the intestinal tissues of dogs infected with leishmania infantum.this study was about a semi-quantitative analysis of t lymphocytes (cd4+ and cd8+, foxp3+ regulatory t cells), and macrophages in the gut wall of dogs naturally infected with leishmania infantum. thirteen dogs were divided into three groups: group 1 (g1, n=5), dogs with canine visceral leishmaniasis (cvl) and infected with l. infantum amastigotes in the intestine; group 2 (g2, n=5), dogs with cvl but without intestinal amastigotes; and group 3 (g3, n=3), uninfected dogs (control group). there wa ...201728746446
strategies for reducing the risk of transfusion-transmitted leishmaniasis in an area endemic for leishmania infantum: a patient- and donor-targeted approach.in the balearic islands, as in other areas of the mediterranean basin, there is a significant proportion of asymptomatic leishmania (l.) infantum-infected blood donors, who may represent an important threat to transfusion safety. the balearic islands blood bank, located in an area endemic for l. infantum, carried out a study of donors and patients to investigate the impact of this infectious disease on blood safety in the region.201728488962
imported leishmaniasis in a dog in a sandfly-populated area in northeastern romania.we report the importation of a clinical case of canine leishmaniasis (canl) in romania, a country where several types of sandflies are present with the potential to develop a new focus of canl. the staffordshire bull terrier dog was imported into romania from spain 1.5 y before he developed clinical symptomatology that included proliferative dermatitis, lymphadenomegaly, and bilateral uveitis. hematologic analyses showed regenerative anemia and subacute inflammation. cytologic examination of lym ...201728480825
in vitro evaluation of cytotoxicity and leishmanicidal activity of phthalimido-thiazole derivatives.it is estimated that the worldwide prevalence of leishmaniasis is around 12 million individuals in 80 countries, with 400,000new cases per year. in the search for new leishmanicidal agents, the hybrid phthalimido-thiazoles have been identified as an important scaffold for drug design and discovery. the present study thus reports the in vitro activity of a series of phthalimido-thiazole derivatives. cytotoxicity against a strain of l. infantum, vero cells, j774 macrophages and peritoneal macropha ...201728478133
prevention of feline leishmaniosis with an imidacloprid 10%/flumethrin 4.5% polymer matrix collar.leishmaniosis caused by leishmania infantum is one of the most important vector-borne diseases affecting animals and humans worldwide. dogs are considered main reservoirs of the zoonotic forms, though in the last years the role of cats as reservoirs has been increasingly investigated. feline leishmaniosis (fel) occurs in endemic areas and no specific preventive measures have been investigated so far. in this study the efficacy of a 10% imidacloprid/4.5% flumethrin polymer matrix collar, licensed ...201728705248
standardization and evaluation of a duplex real-time quantitative pcr for the detection of leishmania infantum dna: a sample quality control approach.molecular techniques have been shown to be alternative methods for the accurate detection of infectious and parasitic diseases, such as the leishmaniases. the present study describes the optimization and evaluation of a duplex real-time quantitative pcr (qpcr) protocol developed for the simultaneous detection of leishmania infantum dna and sample quality control.201728700053
genetic variability of leishmania infantum in naturally infected dogs in the state of bahia, brazil.in brazil, leishmania infantum mainly affects humans and dogs. the state of bahia presents many dogs that are positive for this parasite. despite the importance of epidemiology in this region, there are still very few studies that have assessed the genetic characteristics of l. infantum. the aim of this study is to investigate the genetic variability of l. infantum isolated identified in naturally infected dogs, in order to verify occurrence of subpopulation of this parasite in the different bio ...201728700001
a nitrile glucoside and biflavones from the leaves of campylospermum excavatum (ochnaceae).the study of the meoh extract of the leaves of campylospermum excavatum led to the isolation of a nitrile glucoside, named campyloside c (1) and an original derivative of ochnaflavone, 7-o-methylochnaflavone (2) along with three known biflavonoids, amentoflavone, sequoiaflavone, and sotetsuflavone (3-5). the linkage site of the sub-units of 2 was confirmed by chemical correlation, after semi-synthesis of a trimethoxylated derivative of ochnaflavone (2a). the structure of these compounds as well ...201728695668
diagnostic challenges in the era of canine leishmania infantum vaccines.the diagnosis of canine leishmaniosis (canl) is complex due to its variable clinical manifestations and laboratory findings. the availability of vaccines to prevent canl has increased the complexity of diagnosis, as serological tests may not distinguish between naturally infected and vaccinated dogs. current practices of prevaccination screening are not sufficiently sensitive to detect subclinically infected dogs, resulting in the vaccination of infected animals, which may lead to disease in vac ...201728689776
pegylated dendritic polyglycerol conjugate delivers doxorubicin to the parasitophorous vacuole in leishmania infantum infections.most drugs against visceral leishmaniasis must be administered parenterally. a controlled drug release at the target site can improve the efficacy and toxicity of antileishmanial drugs in clinical use. amastigotes live and grow inside the parasitophorous vacuole of host resident macrophages. therefore, antileishmanial drugs should accumulate in this compartment to kill the parasite and do not produce toxicity to the cell host. pegylated dendritic polyglycerol conjugates (pg-peg) can ensure a con ...201728683183
computer-aided discovery of two novel chalcone-like compounds active and selective against leishmania infantum.leishmaniasis are infectious diseases caused by parasites of genus leishmania that affect affects 12 million people in 98 countries mainly in africa, asia, and latin america. effective treatments for this disease are urgently needed. in this study, we present a computer-aided approach to investigate a set of 32 recently synthesized chalcone and chalcone-like compounds to act as antileishmanial agents. as a result, nine most promising compounds and three potentially inactive compounds were experi ...201728434763
first example of peptides targeting the dimer interface of leishmania infantum trypanothione reductase with potent in vitro antileishmanial activity().a series of 9-mer and 13-mer amide-bridged cyclic peptides derived from the linear prototype ac-pkiiqsvgis-nle-k-nle-nh2 (toro et al. chembiochem2013) has been designed and synthesized by introduction of the lactam between amino acid side chains that are separated by one helical turn (i, i+4). all of these compounds were tested in vitro as both dimerization and enzyme inhibitors of leishmania infantum trypanothione reductase (li-tryr). three of the 13-mer cyclic peptide derivatives (3, 4 and 6) ...201728431354
prevalence and geographic distribution of vector-borne pathogens in apparently healthy dogs in croatia.vector-borne pathogens (vbps) are a group of globally extended and quickly spreading pathogens that are transmitted by various arthropod vectors. the aim of the present study was to investigate the seroprevalence against babesia canis, anaplasma phagocytophilum, borrelia burgdorferi sensu lato, leishmania infantum, dirofilaria immitis, and ehrlichia canis in dogs in croatia. we investigated 435 randomly selected apparently healthy dogs in 13 different locations of croatia for antibodies to b. ca ...201728448211
seroepidemiological survey of human visceral leishmaniasis in ilam province, west of iran in 2013.visceral leishmaniasis (vl) as one of the most important human parasitic disease is endemic in some parts of iran. several cases of vl have been reported recently in the ilam province. the current study aimed to assess the present status of human vl in the region.201725904946
mucocutaneous leishmaniasis caused by leishmania infantum var lombardi in an immunocompetent patient, spain. 201725559703
causes of pediatric visceral leishmaniasis in southeastern iran.leishmania infantum is the most frequent cause of visceral leishmaniasis and l. tropica has been rarely linked to the disease in iran. in this study, bone marrow aspirates were collected from 10 child patients, suspected with visceral leishmaniasis referred to the pediatric wards of kerman medical hospitals, kerman, iran during 2002-2011. leishmania species were identified by using nested pcr in all slides. the pcr samples from nine patients indicated l. infantum as principal causative agent of ...201725759741
molecular characterization of leishmania infantum strains by kinetoplast dna rflp-pcr.multilocus enzyme electrophoresis is the tool most frequently used to classify leishmania spp., although it is time consuming and, sometimes, a not enough discriminative method. in the present study a kinetoplast dna polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) was used to characterize 16 zymodeme mon-1 leishmania infantum strains: 15 were from dogs housed in public kennels of 7 geographical areas in the emilia-romagna region, northern italy, 1 was the l. infantu ...201727033533
antiprotozoal activity of major constituents from the bioactive fraction of verbesina encelioides.the bioactive petroleum ether fraction of verbesina encelioides, previously studied by the authors, was chosen for the isolation of antiprotozoal metabolites. pseudotaraxasterol-3β-acetate (1), benzyl 2,6-dimethoxy benzoate (2), 16β-hydroxy-pseudotaraxasterol-3β-palmitate (3) and pseudotaraxasterol (4), in addition to β-sitosterol glucoside (5) and β-sitosterol galactoside (6) were isolated and identified based on one-dimensional and two-dimensional spectral analysis. this is the first report de ...201727154232
the challenge of stability in high-throughput gene expression analysis: comprehensive selection and evaluation of reference genes for balb/c mice spleen samples in the leishmania infantum infection model.the interaction of leishmania with balb/c mice induces dramatic changes in transcriptome patterns in the parasite, but also in the target organs (spleen, liver…) due to its response against infection. real-time quantitative pcr (qpcr) is an interesting approach to analyze these changes and understand the immunological pathways that lead to protection or progression of disease. however, qpcr results need to be normalized against one or more reference genes (rg) to correct for non-specific experim ...201727668434
immune response to recombinant leishmania infantum lipophosphoglycan 3 plus cpg oligodeoxynucleotides in balb/c mice.development of a protective antileishmanial vaccine is an urgent priority for successful control of different forms of leishmaniasis. the potential of a recombinant lipophosphoglycan 3 (rlpg3) expressed by leishmania tarentolae was evaluated in combination with cpg oligodeoxynucleotides (cpg-odn) as a th1-promoting adjuvant against leishmania infantum infection in balb/c mice. first, mice were immunized subcutaneously with rlpg3 either alone or in combination with cpg-odn. next, the immunogenic ...201727353355
the recombinant protein rsp03b is a valid antigen for screening dog exposure to phlebotomus perniciosus across foci of canine leishmaniasis.the frequency of sandfly-host contacts can be measured by host antibody levels against sandfly salivary proteins. recombinant salivary proteins are suggested to represent a valid replacement for salivary gland homogenate (sgh); however, it is necessary to prove that such antigens are recognized by antibodies against various populations of the same species. phlebotomus perniciosus (diptera: psychodidae) is the main vector of leishmania infantum (trypanosomatida: trypanosomatidae) in southwest eur ...201727718267
canine visceral leishmaniasis in iran: a systematic review and meta-analysis.visceral leishmaniasis is considered an endemic zoonosis in some parts of iran and dogs are main reservoirs, which play role in the transmission cycle of human leishmaniasis. this systematic review and meta-analysis was performed to determine the prevalence of canine visceral leishmaniasis (cvl) in iran. data were systematically collected from 1982 to 2015 in iran on the following electronic databases: pubmed, google scholar, science direct, scopus, web of science, magiran, irandoc, iran medex a ...201727570207
in vitro antileishmanial and antimalarial activity of selected plants of nepal.nepal is very rich in biodiversity, and no extensive effort has yet been carried out to screen plants that are used by traditional healers against parasitic diseases. the aim of this study was to evaluate the in vitro antileishmanial and antimalarial activity of crude methanolic or ethanolic extracts of 29 plant species that are currently used by local people of nepal for treating different ailments.201727757268
a recombinant chimeric protein composed of human and mice-specific cd4(+) and cd8(+) t-cell epitopes protects against visceral leishmaniasis.in this study, a recombinant chimeric protein (rcp), which was composed of specific cd4(+) and cd8(+) t-cell epitopes to murine and human haplotypes, was evaluated as an immunogen against leishmania infantum infection in a murine model. balb/c mice received saline were immunized with saponin or with rcp with or without an adjuvant. the results showed that rcp/saponin-vaccinated mice presented significantly higher levels of antileishmanial ifn-γ, il-12 and gm-csf before and after challenge, which ...201727593711
chroman-4-one derivatives targeting pteridine reductase 1 and showing anti-parasitic activity.flavonoids have previously been identified as antiparasitic agents and pteridine reductase 1 (ptr1) inhibitors. herein, we focus our attention on the chroman-4-one scaffold. three chroman-4-one analogues (1-3) of previously published chromen-4-one derivatives were synthesized and biologically evaluated against parasitic enzymes (trypanosoma brucei ptr1-tbptr1 and leishmania major-lmptr1) and parasites (trypanosoma brucei and leishmania infantum). a crystal structure of tbptr1 in complex with com ...201728282886
insights on adaptive and innate immunity in canine leishmaniosis.canine leishmaniosis (canl) is caused by the parasite leishmania infantum and is a systemic disease, which can present with variable clinical signs, and clinicopathological abnormalities. clinical manifestations can range from subclinical infection to very severe systemic disease. leishmaniosis is categorized as a neglected tropical disease and the complex immune responses associated with leishmania species makes therapeutic treatments and vaccine development challenging for both dogs and humans ...201727094260
serological detection of toxoplasma gondii, leishmania infantum and neospora caninum in cats from an area endemic for leishmaniasis in brazil.an investigation was made into the occurrence of antibodies to toxoplasma gondii, leishmania infantum and neospora caninum in 151 domestic cats, based on the indirect fluorescent antibody test (ifat). serum samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats; 55 males and 96 females) in campo grande, mato grosso do sul, brazil between january and april 2013. igg antibodies to t. gondii, l. infantum and n. caninum were found, respectively, in 49 (32.5%), 34 (22.5% ...201725517522
leishmaniasis in turkey: determination of leishmania species by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms).cutaneous leishmaniasis (cl) is endemic in southeastern anatolia, mainly in sanliurfa and hatay provinces, and the causative agents are mostly leishmania tropica and less frequently l. infantum. here, we report the first maldi-tof analyses of leishmania promastigotes obtained from the cultures of two cl cases from osmaniye and hatay provinces who were initially diagnosed by microscopy, culture and identified as l. infantum with real-time pcr (rt-pcr).201725848391
Refresh
firstprev
...3132
nextlast
Displaying items 3101 - 3165 of 3165