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changes in polyene synthesis induced by mutation in a red yeast (rhodotorula rubra). 194620983286
[effects of ultraviolet irradiation on rhodotorula mucilaginosa; production of various colored mutants, study of their sterol content]. 195513293883
[studies on variation of carotinoid components in rhodotorula rubra in relation to nutritional conditions]. 195713403655
[carotenoids of rhodotorula mucilaginosa. study of their biosynthesis with the aid of analysis of mutants and the use of an inhibitor of carotenogenesis]. 196013842320
[influence of various chemical agents on carotenogenesis in rhodotorula mucilaginosa]. 196013842321
[absence of influence of changes in carotenogenesis on the growth and respiratin of rhodotorula mucilaginosa]. 196013752055
[biosynthesis of carotenoids by rhodotorula mucilaginosa. study on a mutant obtained by the action of x-rays]. 196114454689
[the action of beta-ionone on carotenoid synthesis in rhodotorula rubra]. 196213923501
production of egg yolk coloring material by a fermentation process.rhodotorula mucilaginosa was cultivated in a yeast propagator, for the production of biomass and carotenoids. a spray-dried preparation of the yeast was incorporated into the diets of laying chickens for the promotion of egg yolk color. several aspects of the effect of media on growth and carotenogenesis of pigmented yeast were examined. addition of 1 to 2% of rhodotorula yeast to the feeding mixture considerably improved egg yolk color. the possibility of using carotenogenic yeasts as a feed su ...19655893694
[the biosynthesis of acyclic carotenoids in a mutant of rhodotorula mucilaginosa]. 19676069106
[effect of the genetically active and carcinogenic agent 1-nitroso-imidazolidone-2 on the fine structure of the yeast rhodotorula rubra]. 19685693445
[a study of carotenoid pigments of 2 rhodotorula rubra strains]. 19685753520
microbial transformation. i. microbial transformation of androstenedione and testosterone by rhodotorula mucilaginosa (jörg) harrison. 19695374566
biological assays for two mycotoxins produced by fusarium tricinctum.a survey was made to detect microorganisms useful for assaying butenolide [4-acetamido-4-hydroxy-2-butenoic acid gamma-lactone] and t-2 toxin [4beta, 15-diacetoxy-8alpha-(3-methylbutyryloxy)-12,13-epoxytricothec -9-en-3alpha-ol]. these mycotoxins produced by strains of fusarium tricinctum have been implicated in mycotoxicosis of livestock. although butenolide proved to be a very weak antibiotic, assay discs containing 100 mug of this toxin inhibited sprillum serpens nrrl b-2052, vibrio tyrogenus ...19705485724
rhodotorula mucilaginosa dermatitis on feathered parts of chickens: an epizootic on a poultry farm. 19705464037
aspects of the stereochemistry of torularhodin biosynthesis.1. the incorporation of [2-(14)c]acetate, [2-(14)c]mevalonic acid and [2-(14)c,2-(3)h(2)]-mevalonic acid into torulene and torularhodin by rhodotorula rubra and rhodotorula glutinis was studied. 2. a recovery of 14.3% of the label was obtained on decarboxylation of the torularhodin biosynthesized from [2-(14)c]mevalonic acid. 3. an analysis of the (3)h/(14)c ratio in torularhodin gave a value of 9.44:8. 4. these results, obtained by different experimental techniques, show that the reactions in t ...19705465554
aspects of mitochondriogenesis in yeast. ii. the effect of chloramphenicol on rhodotorula mucilaginosa. 19715105336
correlation of biological to chromatographic data for two mycotoxins elaborated by fusarium.thirty-seven identified strains of fusarium, most of them isolated from fescue grass, were tested for their ability to elaborate mycotoxins in laboratory culture. the presence of the toxins was determined by infrared light, thin-layer chromatography, mouse toxicity, fungistatic effects, and phytotoxic properties. a good correlation was demonstrated between t-2 toxin detection by thin-layer chromatography and inhibition of rhodotorula rubra by culture extracts. all of the strains producing either ...19715575569
[regulation of urease synthesis in rhodotorula mucilaginosa]. 19724672420
survey for fusaria that elaborate t-2 toxin.of the 136 strains of fusarium examined, t-2 toxin was confirmed by thinlayer chromatography in 13 of the 21 extracts that inhibited either rhodotorula rubra or pencillium digitatum.19725064987
a method for assaying activity and some other properties of nadp-oxidoreductase from rhodotorula mucilaginosa. 19734147775
a hydroxamic acid present in rhodotorula pilimanae cultures grown at low ph and its metabolic relation to rhodotorulic acid. 19734686797
continuous culture of rhodotorula rubra: kinetics of phosphate-arsenate uptake, inhibition, and phosphate-limited growth.the pink yeast rhodotorula rubra of marine origin was found to be capable of extended growth at very low phosphate concentrations (k(0.5) = 10.8 nm). average intracellular phosphate concentrations, based on isotope exchange techniques, were 15 to 200 nm, giving concentration gradients across the cell envelope of about 10(6). sensitivity to metabolic inhibitors occurred at micromolar concentrations. inability of the phosphate transport system, k(s) = 0.5 to 2.8 mum, v(max) = 55 mumoles per g of c ...19734690960
regulation of aromatic metabolism in fungi: selection of mutants of the yeast rhodotorula mucilaginosa with nystatin. 19744474355
regulation of aromatic metabolism in the fungi: metabolic control of the 3-oxoadipate pathway in the yeast rhodotorula mucilaginosa. 19744474356
the atmospheric fungal flora of the athens metropolitan area.in the research programme of the department of microbiology of the athens university the nature of the mycological flora of the athenian air was studied. the research took place during the calendar year 1971. the open air was sampled twice weekly from two observation stations. the open plate technique was used, petri dishes containing sabourand's agar being exposed for 15 minutes. a total of 180 plates were exposed, and 1714 fungal colonies were isolated; these were subcultured and identified as ...19751207717
[effect of yeast mannans on aldolase of rat blood serum].the activity of aldolase in rat blood serum was studied in experiments in vitro and in vivo as affected by extracellular mannan of rhodotorula rubra. it was established that in vitro the polysaccharide has an inhibitory effect in the enzyme and in vivo, vice versa, activates it. the maximal activation is observed against a background of the v increase and km decrease. by the preparative electrophoresis in 7.5% polyakrylamide gel seven isoenzymes of aldolase are obtained. after introduction of th ...19751209756
[effect of yeast mannan on the activity and kinetic patterns of alpha amylase from rat blood serum].the mannan from rhodotorula rubra activated alpha-amylase of blood serum in vivo. after intravenous administration of the polysaccharide into rats kinetic patterns of the enzyme were altered. the effect of mannan on various molecular forms of alpha-amylase was also studied.19751216753
rhodotorula rubra--cause of eye infection. 19751169685
endocarditis caused by rhodotorula successfully treated with 5-fluorocytosine.a 7-year-old boy suffering from aortic regurgitation and mitral stenosis and regurgitation was admitted with endocarditis caused by rhodotorula pilimanae and was treated successfully with orally administered antifungal agent, namely 5-fluorocytosine (5-fc). a dose of 100 mg per kg body weight, divided into four equal parts, was prescribed. after a prolonged febrile period his temperature dropped to normal on the fourth day of 5-fc therapy. review of the published reports disclosed few cases of e ...19751167783
[kininases from yeasts (author's transl)].practically no studies are available on the presence of kinin-decomposing enzymes in yeasts. different preparations (intact washed cell suspensions, cell-homogenised blastospores, nutrient media, supernatant of raw fungus suspensions) of candida strains sampled from foci of disease and from the environment were studied qualitatively and quantitatively fro the presence of kinin-inactivating enzymes. the parameter measured is the time-dependent inactivation of bradykinin by the test strain prepara ...19751154909
mycotic flora of the interdigital spaces of the human foot: a preliminary investigation.a qualitative and quantitative study of the mycotic flora of the interdigital spaces of 27 male volunteers yielded 1,291 moulds and 598 yeasts. concurrently, a study of garden soil was conducted in order to obtain data concerning the transient-resident status of the fungi recovered from the feet. of the 120 genera and species of fungi isolated, 51 were recovered from the volunteers, 53 from the soil, and 16 from both categories. the most commonly recovered fungi from the toewebs, in order to occ ...19751143314
aromatic metabolism in the fungi. growth of rhodotorula mucilaginosa in p-hydroxybenzoate-limited chemostats and the effects of growth rate on the synthesis of enzymes of the 3-oxoadipate pathway.rhodotorula mucilaginosa was grown in p-hydroxybenzoate-limited chemostats over the dilution rate (d) range 0.01 to 0.17 h-1 and growth was adequately described by the monod theory when maintenance energy requirements were considered. the p-hydroxy-benzoate affinity constant, k8, had the relatively high value of 270 mg/i. the yield from p-hydroxbenzoate varied with dilution rate but was constant above d equal 0.07 h-1 at 0.56 g yeast/g substrate utilised. the maintenance coefficeint for growth o ...19751090273
the distribution of pyrophosphatidic acid in nature.the occurrence of a novel phospholipid, pyrophosphatidic acid, in the lipid extracts of yeasts (23 species), bacteria (e. coli), algae (chlorella), mammalia (human, rabbit, guinea pig, and mouse), insect (cockroach), fish (carp), mollusc (clam), and spermatophyta (spinach) was investigated. pyrophosphatidic acid was found exclusively in the lipid extracts of several kinds of yeast species, but not in other normal living species (animals, plants, and microorganisms) so far investigated. all of th ...1975175047
meningitis caused by rhodotorula.a case of rhodotorula meningitis in a 21 year old male compromised with acute lymphoblastic leukemia is reported. the yeast was identified as rhodotorula rubra (demme) lodder.19761069381
lipid composition of 30 species of yeast.the detailed composition of cellular lipid of more than 23 species of yeast has been determined quantitatively by thinchrography on quartz rods, a method previously used for estimating cellular lipids of seven species of yeast. that data was fortified by neutral and phospholipid quantitations on 30 species of yeast cells. most of the test organisms contained 7-15% total lipid and 3-6% total phospholipid per dry cell weight, except for the extremely high accumulation of triglycerides in two speci ...19761011938
[effect of rhodotorula rubra yeast mannan on the molecular heterogeneity of rat blood serum aldolase in the dynamics of tumor growth]. 1976946917
[study of the rhodotorula rubra cell wall. vii influence of chloramphenicol on its morphology and phosphatase activity (author's transl)].rh. rubra grown in the presence of chloramphenicol (500 mug/ml of medium) displayed morphological and biological changes, including formation of aberrant mitochondria with altered cristae, increase of the ratio fundamental element/mucilaginous sheath and electron-transparent regions in the cell walls. after treatment of the yeast with trypsin or beta-glucanase complex, acid and alkaline phosphatases remained localised within the cell wall, but the effect of the degradative enzymes on the activit ...1976943241
[studies on the cell wall of yeast of the genus rhodotorula. x. isolation and purification of cell-wall glycoproteins from rhodotorula rubra (author's transl)].following ethylene diamine treatment of the cell wall of rhodotorula rubra, a water soluble fraction has been isolated. this fraction can be resolved into three glycoproteins and one protein. the major part is a glycoprotein, purified to homogeneity which has a molecular weight of 64 000. the glyco-part of this protein contains mannose, glucose and one residue of glucosamine. after pronase treatment, the presence of an ""asparaginyl-n-acetylglucosamine" linkage is established by the existence of ...1976943181
the development of new iron-chelating drugs.2,3-dihydroxybenzoic acid has been identified as a potentially useful iron-chelating drug. accordingly, we have evaluated a series of derivatives of hydroxylated benzoic acids for their ability to induce iron excretion in the iron-overloaded rat. in addition, we have examined a number of hydroxamic acids and some other naturally occurring iron-chelating agents. of the 26 benzoic acid derivatives studied, none appeared to be more effective than 2,3-dihydroxybenzoic acid, for reasons which are dis ...19761255491
acid phosphatase of the yeast rhodotorula rubra. purification and properties of the enzyme.1. acid phosphatase from the yeast rhodotorula rubra was purified 44-fold. the purification procedure involved mechanical disruption of cells, precipitation with ethanol, chromatography on deae- and cm-cellulose. 2. the purified enzyme is homogeneous in polyacrylamide gels at ph 4.5, 9.5 and 8.4. carbohydrate content accounts for 57% of the total weight. the optimum ph is at 4.0-4.6, and the enzyme is stable over ph range from 2.6 to 6.0. full activity was retained on 60-min incubation at 50 deg ...197717985
[infrared spectrum of yeast cell walls].infrared spectra of whole cells and cell walls were studied with schizosaccharomyces pombe, endomyces magnusii. rhodotorula rubra, and candida lipolytica. qualitative analysis of the spectra and comparison of the spectra recorded for the cells and for their walls led to a conclusion that, contrary to the common viewpoint, an ir spectrum of the whole cell of any yeast species was determined by the components contained in the cell rather wall than by all components of the cell.1977895561
taxonomic study and fermentation of producing organism and antimicrobial activity of mildiomycin.a taxonomic study of strain b-98891, which produced an antibiotic effective against powdery mildew of barley, identified it as streptoverticillium rimofaciens. on agar media the antibiotic, which was named mildiomycin, was only weakly active against most fungi and bacteria tested. however, it inhibited some mycobacterium and rhodotorula, and it showed excellent control of powdery mildew of barley plants in greenhouse tests at concentrations between 31.2 and 62.5 ppm. rhodotorula rubra ifo 0907 w ...1978681232
coordination chemistry of microbial iron transport compounds: rhodotorulic acid and iron uptake in rhodotorula pilimanae.the mechanism by which iron uptake is facilitated by the siderophore rhodotorulic acid (ra) in the yeast rhodotorula pilimanae was investigated with radioactively labeled fe and ra and kinetically inert, chromic-substituted ra complexes. the weight of the evidence supports a model in which ra mediates iron transport to the cell but does not actually transport iron into the cell. it is proposed that ra exchanges the ferric ion at the cell surface with a membrane-bound chelating agent that complet ...197830750
[lipolytic activity of osmotolerant yeasts].the ability to hydrolyse olive oil, cod-liver oil, caviar oil, and yeast oil was studied in osmotolerant yeasts candida mogii 2, debaryomyces hansenii 8, and rhodotorula rubra 9, isolated from salted caviar. the lipolytic activity was manifested at 5, 15 and 26 degrees c and ph 4.0--9.2, even in the presence of high concentrations (0.5--3 m) of such chlorides as kcl, nacl and cacl2. the lipolytic activity of s variant of d. hansenii 8 was higher than the activity of its r variant.1978661632
taxonomic implications of rhodotorula rubra isolates from polluted sea water in rio de janeiro, brazil.sixty two strains of rhodotorula rubra which were all isolated from marine and estuarine waters of rio de janeiro were found variable for 19 to 32 carbon assimilation tests used in yeast taxonomy. two r. rubra strains which had latent assimilation of maltose and melizitose appeared to be intermediate with r. pilimanae. strains of r. glutinis var dairenensis which differed from r. rubra only by weak assimilation of nitrate appeared to be intermediated between these two species. our physiological ...1979572568
phosphate-limited continuous culture of rhodotorula rubra: kinetics of transport, leakage, and growth.the phosphate-limited growth kinetics of rhodotorula rubra, a small yeast of marine origin, were examined by analysis of 32p distributions in continuous cultures. isotope relaxation procedures were used to identify unidirectional flows of pi and organic phosphate among compartments modeled during growth. the concentrations of phosphates in these compartments at various growth rates were used, together with attendant flows, to produce a mathematical model of growth. both pi and phosphate-containi ...197937231
microbiological transformations. xi. the use of immobilized rhodotorula mucilaginosa cells to reduce some ketones.rhodotorula mucilaginosa cells were immobilized by polymerizing acrylamide with the addition of a cross-linked agent, a catalyst and an initiator. this gel was used for the stereospecific reduction of acetophenone and alpha-acetylnaphthalene resulting chromatographically pure alcohols with absolute configuration s. when karen-3-dion-2,5 was used as substrate, four products were obtained. androstenolone was reduced with 30% yield. activity of the immobilized cells could be regenerated by glucose.1979157727
the fine structure of psychrophilic and mesophilic rhodotorula rubra.transmission electron microscopy of thin sections of two mesophilic and a psychrophilic rhodotorula rubra revealed structures normally associated with yeast. the capsule of all three strains was thicker in the cells grown in glucose than those grown in glucose-free media. the cell wall of all three strains showed more lamellae in the cells grown in a medium containing glycerol. budding was of the rhodoturula type. endoplasmic reticula running parallel to the nucleus were commonly observed in the ...1979575628
reversible bioconcentration of monochlorobiphenyls by rhodotorula rubra: correlations with aqueous solubility of substrate. 1979574035
ovine pulmonary infection caused by rhodotorula rubra. 19807402216
phenylalanine ammonia-lyase entrapped in fibers.phenylalanine ammonia-lyase extracted form rhodotorula rubra (ifo 1101) was immobilized into cellulose triacetate fibers made hemocompatible by physical blend with a platelet anti-aggregating agent. the entrapped enzyme could operate at physiological values of phenylalanine and tyrosine reducing their level to traces within a few hours. the optimum ph value for the entrapped enzyme shifted from 8.0 to 9.0. at blood ph the activity was about 68 per cent of the maximum. the entrapped enzyme retain ...19807417592
characterization of a novel l-asparaginase produced by rhodotorula rubra. 19807027389
[thermal resistance of candida tropicalis and rhodotorula rubra in orange juice].the thermal resistance of two yeasts (candida tropicalis and rhodotorula rubra) was studied. the yeasts were isolated from venezuelan pasteurized orange juice obtained at retail level. the flask method was utilized, applying temperatures which ranged from 49.5 to 53.5 degrees c to initial populations of 1x10(5) cells/ml of orange juice. values of d50c=3.4 min and z=4.1 degrees c were found for c. tropicalis, and d50c=2.6 min and z=9.8 degrees c for r. rubra. linear correlation coefficients of r= ...19817201301
[aggregation and separability of the shikimate pathway enzymes in yeasts].gel filtration was employed to estimate the molecular weights and to determine possible physical aggregation of enzymes [5-dehydroquinate synthase (dhq synthase), 5-dehydroquinase (dhqase, ec 4.2.1.10), shikimate: nadp oxidoreductase (ec 1.1.1.25), shikimate kinase (ec 2.7.1.71), 3-enolpyruvylshikimate 5-phosphate synthase (epsp synthase)] in the shikimate pathway in eleven species of yeasts. the five enzymes were not aggregated in extracts of hansenula henricii, h. fabianii, h. anomala, candida ...19816270920
occurrence and growth of yeasts in yogurts.yogurts purchased from retail outlets were examined for the presence of yeasts by being plated onto oxytetracycline malt extract agar. of the 128 samples examined, 45% exhibited yeast counts above 10 cells per g. a total of 73 yeast strains were isolated and identified as belonging to the genera torulopsis, kluyveromyces, saccharomyces, candida, rhodotorula, pichia, debaryomyces, and sporobolomyces. torulopsis candida and kluyveromyces fragilis were the most frequently isolated species, followed ...198116345853
competition between heterotrophic and autotrophic microplankton for dissolved nutrients.when a heterotrophic (rhodotorula rubra) and a phototrophic (selenastrum capricornutum) plankton were grown together in dilute phosphate (pi) continuous cultures, coexistence occurred only when the heterotroph was growthrate limited by organic carbon (c). because of its higher affinity for pi, and because c starvation does not affect the heterotrophic yeast's ability to transport pi, the concentration of organic carbon indirectly controlled the biomass of the phototroph. the results support a th ...198124227495
[persistent localized hyperkeratosis caused by rhodotorula rubra (author's transl)]. 19827075845
yeasts in the intertidal sediments of a polluted estuary in rio de janeiro, brazil.yeasts were found concentrated at the sediment surface and with the highest counts at the most polluted site. candida krusei, pichia membranaefaciens and similar species typically forming rugose colonies with radiating ridges were the prevalent yeasts in these sediments, and species like rhodotorula rubra related to basidiomycetous fungi were found in relatively low numbers.19827092202
[arthrobacter globiformis - a new yeast-lysing bacterium].a bacterium capable to lyse viable yeast cells was isolated from compost enriched with baker's yeast cells and was identified as arthrobacter globiformis. the yeast lytic enzyme complex produced by shaking culture was precipitated with ammonium sulfate, dialyzed and lyophilized. the crude residue contained beta-glucanase and alpha-mannanase, yet not chitinase. optimale carbon sources in the culture medium for a high enzyme synthesis were 0.5% beta-glucan for the production of beta-glucanase, res ...19827157841
rhodotorula rubra peritonitis in patients undergoing continuous ambulatory peritoneal dialysis.although still uncommon, fungal peritonitis is being reported with increasing frequency in patients with renal failure who are undergoing long-term peritoneal dialysis. although most cases have involved candida albicans, other opportunistic pathogens have been reported as well. recently, three patients were seen with serious peritoneal infections due to rhodotorula rubra, a "nonpathogenic" fungus. in two patients, peritoneal fibrosis made further peritoneal dialysis impossible. the third patient ...19836881189
contribution to the study of the enzymatic profiles of yeast organisms with medical interest.the enzymatic profiles of several yeastlike organisms were studied using 19 substrates included in the api zym system. the isolates evaluated were: 186 candida albicans, 19 c. stellatoidea, 4 c. tropicalis, 2 c. parapsilosis, 2 c. pseudotropicalis, 1 c. guilliermondii, 3 c. krusei, 11 torulopsis (candida) glabrata, 1 cryptococcus neoformans, 2 saccharomyces carlsbergensis, 1 rhodotorula rubra, and 1 r. mucilaginosa. esterase lipase (c8), leucine arylamidase, acid phosphatase, and phosphoamidase ...19836310400
dissemination of yeasts after gastrointestinal inoculation in antibiotic-treated mice.mice pretreated with antibiotics were inoculated intragastrically with different yeast isolates to determine whether the resulting disruption of the normal flora ecology would allow certain fungi to colonize and disseminate from the gastrointestinal tract. antibiotic treatment decreased the total population levels of the indigenous bacterial flora, and predisposed mice to gastrointestinal overgrowth and subsequent dissemination by candida albicans, c. parapsilosis, c. pseudotropicalis, c. tropic ...19836342174
serological differences between the multiple amine oxidases of yeasts and comparison of the specificities of the purified enzymes from candida utilis and pichia pastoris.1. antiserum to purified methylamine oxidase of candida boidinii formed precipitin lines (with spurs) in double-diffusion tests with crude extracts of methylamine-grown cells of the following yeast species: candida nagoyaensis, candida nemodendra, hansenula minuta, hansenula polymorpha and pichia pinus. no cross-reaction was observed with extracts of candida lipolytica, candida steatolytica, candida tropicalis, candida utilis, pichia pastoris, sporobolomyces albo-rubescens, sporopachydermia cere ...19836409096
a rapid and sensitive paper electrophoresis assay for the detection of microbial siderophores elicited in solid-plating culture.a rapid and sensitive assay for the detection of microbial siderophores (iron-binding compounds) is described. nine representative fungal and bacterial cultures including ustilago sphaerogena, penicillium sp., fusarium roseum, rhodotorula pilimanae, bacillus subtilis w 23, bacillus subtilis w 168, bacillus megaterium, azotobacter vinelandii op, and escherichia coli b, were nutritionally stressed for iron by sequential transfers on iron-deficient solid-plating media. in response to fe-stress cond ...19836614486
continuous transformation of benzaldehyde to benzyl alcohol by rhodotorula mucilaginosa immobilized in an ultrafiltration cell.microbiological transformation of benzaldehyde accomplished by the fungus rhodotorula mucilaginosa immobilized in the ultrafiltration cell was studied. a polysulfone membrane formed on a sintered pvc support was used for the separation of the transformation product from the cellular material. kinetic investigations have led to results which are typical of continuously fed stirred tank reactors (cfstr)-the value of the maximum reaction rate (v(max)) and apparent michaelis constant (k'(m)) are pra ...198318551433
mycoflora of the human dermal surfaces.the mycotic flora of the scalp and interdigital areas of the hand and foot of 1296 apparently healthy human inhabitants of three amazonian communities were surveyed by means of microscopic examination of epidermal scrapings and cultural isolation on mycosel agar. no macroscopic or microscopic evidence of fungal infection was detected in any of our study subjects. from 133 (10%) individuals, 143 fungi representing 13 genera and 39 species were recovered. yeasts constituted 85% of the fungi. seven ...19846509388
microbial models of mammalian metabolism: microbial reduction and oxidation of pentoxifylline.fourteen microorganisms, including fungi, yeasts, and bacteria, were screened for their ability to metabolize the xanthine drug pentoxifylline. thirteen cultures either reduced the drug to the alcohol metabolite or oxidatively cleaved the ketonic side chain to homologous carboxylic acid metabolites. the alcohol metabolite was the predominant or sole metabolite in all organisms, with conversions ranging from 6 to 91%. preparative-scale production of the alcohol metabolite with rhodotorula rubra ( ...19846486781
[effect of solid substrates of microbial colony development].bacteria have been found to grow differently at contact sites to both organic and anorganic solids as on pure agar media. of 16 ad hoc selected bacterial strains, the following species (arranged in decreasing order) responded with stronger colony formation: bacillus subtilis, b. cereus, streptococcus sanguis, s. saprophyticus, s. faecalis, escherichia coli, micrococcus flavus, actinomyces viscosus, veillonella sp., enterobacter liquefaciens, yersinia enterocolitica. lactobacillus casei behaved n ...19846326398
sensitivity of heat-stressed yeasts to essential oils of plants.eight strains of yeasts (candida lipolytica, debaryomyces hansenii, hansenula anomala, kloeckera apiculata, lodderomyces elongisporus, rhodotorula rubra, saccharomyces cerevisiae, and torulopsis glabrata) were examined for changes in sensitivity to eight essential oils of plants (allspice, cinnamon, clove, garlic, onion, oregano, savory, and thyme) after being sublethally heat stressed. with the exception of garlic oil for all test yeasts, onion oil for s. cerevisiae, and oregano oil for r. rubr ...19846712207
ecology of pathogenic yeasts in amazonian soil.in an investigation of amazonian soil as a natural reservoir for pathogenic fungi, 1,949 soil samples collected from diverse geographical and ecological settings of the brazilian amazon basin were analyzed for the presence of non-keratinophilic fungi by the indirect mouse inoculation procedure and for the presence of keratinophilic fungi by the hair bait technique. all soil samples were acidic with low ph values. from 12% of the soil samples, 241 yeast and yeastlike isolates pertaining to six ge ...19846538774
dissimilation of aromatic compounds in rhodotorula graminis: biochemical characterization of pleiotropically negative mutants.microorganisms oxidize many aromatic compounds through the dihydroxylated intermediates catechol and protocatechuate and through the beta-ketoadipate pathway. the catabolic sequences used by the yeast rhodotorula graminis for the dissimilation of aromatic compounds were elucidated after biochemical analysis of pleiotropically negative mutant strains. growth properties of one mutant strain revealed that benzoate-4-hydroxylase was required for the utilization of phenylalanine, mandelate, and benzo ...19846542098
elicitor-induced accumulation of acridone alkaloid epoxides in ruta graveolens suspension cultures.light and dark grown suspension cultures of ruta graveolens produced small amounts (1-50 microg/g dry weight) of the antimicrobial alkaloids rutacridone epoxide and hydroxyrutacridone epoxide. acrid-one epoxide accumulation could be increased up to a 100 fold within 72 h by elicitation through addition of a suspension of either living free or immobilized yeasts, of dead rhodotorula rubra cells or crude cell wall fraction. chitosan and alginate, exhibiting elicitor properties in other systems, sc ...198417340365
[lytic activity against yeasts in bacillus brevis culture broth].the cultural broth of bacillus brevis, strain 5/4, is capable of lysing the cell walls of rhodotorula rubra, candida utilis and other yeasts. the lytic action of the cultural broth is ascribed to proteolysis.19853900650
microbial models of mammalian metabolism: stereospecificity of ketone reduction with pentoxifylline.the absolute configuration of pentoxifylline alcohol produced by the microbial reduction of pentoxifylline using rhodotorula rubra (atcc 20129) was determined by o.r.d. spectroscopy and p.m.r. studies on the r-(+)-alpha-methoxy-alpha-(trifluoromethyl) phenylacetate (mtpa) ester. the development of a reverse-phase h.p.l.c. method for resolving the diastereomeric r-(+)-mtpa esters of racemic pentoxifylline alcohol and assignment of the elution order, allowed for the determination of the stereochem ...19853911603
the mechanism and specificity of iron transport in rhodotorula pilimanae probed by synthetic analogs of rhodotorulic acid.the yeast rhodotorula pilimanae produces the dihydroxamate siderophore rhodotorulic acid (ra) in prodigious amounts when starved for iron. synthetic dihydroxamate analogs of ra have been prepared in which the diketopiperazine ring of ra is replaced by a simple chain of n methylene groups. it is found that r. pilimanae is able to accumulate iron using these achiral complexes, as well as from simple monohydroxamate analogs, at rates comparable to those of ra. while the fe2ra3 complex does not ente ...19854055765
stereospecificity of siderophore-mediated iron uptake in rhodotorula pilimanae as probed by enantiorhodotorulic acid and isomers of chromic rhodotorulate.rhodotorulic acid (ra), a dihydroxamate siderophore produced by rhodotorula pilimanae, forms 3:2 complexes with ferric and chromic ions (m2ra3) at ph 7. kinetically inert chromic complexes of ra have been separated into geometrical isomers and for the first time partially resolved into optical isomers. the three isomers delta-cis, delta-trans, and lambda-trans were characterized by their visible and circular dichroism spectra. inhibition by both delta-isomers of radiolabeled ferric ra uptake in ...19854055766
identification and characterization of mitochondrial translation products in various yeasts and in prototheca zopfii.mitochondrial genomes of different eucaryotes are not all alike. we have examined mitochondrial translation products in a number of yeasts (candida krusei, hansenula saturnus, rhodotorula glutinis, rhodotorula rubra, torulopsis glabrata and saccharomyces cerevisiae) and in prototheca zopfii, a colorless alga, in order to determine whether certain proteins are invariably synthesized within mitochondria, how different these proteins are, and what additional proteins, if any, might be synthesized b ...19852836096
[study on the presence of yeasts in the feces of the rock pigeon (columba livia gmelin 1789) from rural areas].the diffusion of rock-pigeon (domestic form of columba livia gmelin 1789) is greatly increasing owing to its high reproductivity; for this reason it is present in both countries and cities. this bird is considered an important vector of pathogens to man and domestic animals, and today it represents a serious hygienic-sanitary problem. therefore we checked for the presence of yeasts in feces of rock-pigeons nesting in the regional park "la mandria" (near turin), visited mostly by school-children ...19853870648
a new type of methylamine oxidase: the sole oxidase produced during growth of sporobolomyces albo-rubescens on primary alkylamines.under conditions known to separate methylamine oxidase from benzylamine oxidase in other yeast strains, only a single oxidase could be detected in sporobolomyces albo-rubescens. this occurred irrespective of whether methylamine or n-butylamine was the nitrogen source for growth. the oxidase did not attack benzylamine. it was concluded that this organism can only produce a methylamine oxidase. the enzyme was purified to 90% homogeneity and found to have properties significantly different from the ...19863505743
orthogonal-field-alternation gel electrophoresis banding patterns of dna from yeasts.chromosomal dnas from various yeast species were separated by orthogonal-field-alternation gel electrophoresis (ofage). to this end we developed a spheroplasting and lysis method to obtain intact dna from both ascomycetous and basidiomycetous yeasts. the ofage banding patterns of 22 ascomycetous and four basidiomycetous yeast strains were compared. the strains represented species from the genera: brettanomyces, candida, cryptococcus, filobasidiella, geotrichum, hansenula, kluyveromyces, pachysol ...19863333308
an improved method for protoplast formation and its application in the fusion of rhodotorula rubra with saccharomyces cerevisiae.protoplasts from various strains of red-pigmented yeasts were generated at high frequency using improved procedures. the use of sulphur-containing amino acids and 2-deoxyglucose in growth media led to impaired cell wall synthesis and rendered cells very susceptible to treatment with mercapto-ethanol and various lytic enzymes. use of individual lytic enzymes separately resulted in relatively low frequencies of protoplasts from most of the red yeasts examined, whilst use of beta-glucuronidase, nov ...19873307677
growth hormesis: a by-product of control.data from experiments, in which colonies of a hydroid, laomedea flexuosa, were exposed to a range of cu2+ concentrations and a marine yeast, rhodotorula rubra, was exposed to a range of cd2+ concentrations, not only exhibit hormesis, but also suggest how its occurrence in growth experiments might be explained. when growth data are considered as normalized specific rates against a time base, their oscillatory form indicates the output of a growth regulatory mechanism whose behaviour can be used t ...19872883157
some properties of extracellular acetylxylan esterase produced by the yeast rhodotorula mucilaginosa.the red yeast rhodotorula mucilaginosa produced an esterase that accumulated in the culture supernatant on induction with triacetin. the enzyme was specific for substrates bearing an o-acetyl group, but was relatively nonspecific for the rest of the molecule, which could consist of a phenol, a monosaccharide, a polysaccharide, or an aliphatic alcohol. the esterase was more active against acetylxylan and glucose beta-d-pentaacetate than were a number of esterases from plant and animal sources, wh ...198716347498
strain improvement of rhodotorula graminis for production of a novel l-phenylalanine ammonia-lyase.l-phenylalanine ammonia-lyase (pal; ec 4.3.1.5) from rhodotorula rubra has been used in the commercial manufacture of l-phenylalanine from trans-cinnamic acid. in this study, r. graminis pal was investigated. mutant strain gx6000 was isolated after ethyl methanesulfonate mutagenesis of wild-type r. graminis gx5007 by selecting for resistance to phenylpropiolic acid, an analog of trans-cinnamic acid. mutant strain gx6000 produced inducible pal at levels four- to fivefold higher than had wild-type ...198816347620
[experimental studies relative to the persistence of several facultative pathogenic fungi in river water].under laboratory conditions survival time of 3 fungi species (candida albicans, geotrichum candidum, rhodotorula rubra) in river water of various quality and temperature has been studied. the mean (and the maximum) survival time was as follows: 82 (240) d for c. albicans, 210 (578) d for g. candidum and 333 (606) d for r. rubra. differences concerning survival time were recorded in dependence on temperature and quality of water. all the 3 strains were multiplied periodically. variants of culture ...19883066060
formation of a biotin precursor, pimelic acid, in yeasts from c18 fatty acids.the biosynthesis of biotin-vitamers from c18 fatty acids in the resting cell reaction system of yeasts was investigated. the formation of pimelic acid (a biotin precursor) and biotin-vitamers (a mixture of 7-keto-8-aminopelargonic acid and dethiobiotin) from linolenic, linoleic, and oleic acids was observed in certain yeasts belonging to the genera cryptococcus, candida, rhodotorula, and trichosporon. metabolites from linolenic acid were analyzed by gas-liquid chromatography and mass spectrometr ...19883236079
rhodotorula rubra peritonitis in a child undergoing intermittent cycling peritoneal dialysis. 19883335817
[yeasts utilizing the surface-active substance laurox-9].yeasts capable of utilizing the non-ionogenic surfactant laurox-9 were identified as cryptococcus humicolus (daszewska) golubev and rhodotorula mucilaginosa (jöerg) harrison. ir spectrometry used for the quantitative assay of laurox-9 showed that r. mucilaginosa utilized it as a sole carbon source. the first step of laurox-9 metabolism was its hydrolytic cleavage yielding lauric acid and polyethyleneglycol. optimal conditions were found for the electron-cytochemical localization of hydrolase inv ...19883419368
[mannan sulfates--inducers of plant resistance to viral infection].mannan sulphates (ms) synthesized on the basis of extracellular linear mannan (lm) of rhodotorula rubra induce resistance of immune-580 tobacco and thornapple (datura stramonium) to tobacco mosaic virus (tmv). the resistance is manifested in a decrease in the number and/or size of viral local lesions (ll) in ms-treated (mg/ml) leaf halves. the reduction in the ll size does not seem to be due to the direct inhibition of tmv multiplication by the polysaccharide as the virus accumulation in the tis ...19883247692
rhodotorula rubra ventriculitis.we describe a case of post-operative ventriculitis in an immunocompetent patient caused by an unusual organism, namely rhodotorula rubra. the patient was treated successfully with antifungal agents.19883351318
[study of the fractions and fibrinolytic activity of fungal mannan].characteristics of the fraction composition of extracellular mannan produced by rhodotorula rubra are presented. various lots of the polysaccharide mainly contained two fractions similar by their chemical structures and differing in the solution relative viscosity. hplc was used for determining the molecular weight of the samples. in the isolated fractions it differed 3-5 fold. relationship between the fibrinolytic activity of the polysaccharide and its molecular weight was revealed. the samples ...19883415392
nucleotide sequence of gene for phenylalanine ammonia-lyase from rhodotorula rubra. 19883205749
the microbial ecology of soybean soaking for tempe production.soybeans soaked in tap water for 24 to 36 h at 20, 30 or 37 degrees c underwent a natural fermentation that was characterized by the growth of microorganisms to 10(8)-10(10) cfu/ml (depending on temperature) and a reduction of ph from 6.5 to 4.5. lactobacillus casei, streptococcus faecium, staphylococcus epidermidis and streptococcus dysgalactiae dominated the fermentation but, significant contributions were also made by klebsiella pneumoniae, klebsiella ozaenae, enterobacter cloacae, enterobact ...19892518226
31p-nmr evidence for cytoplasmic acidification and phosphate extrusion in syringomycin-treated cells of rhodotorula pilimanae.31p-nmr spectroscopy was used to investigate the effects of the phytotoxin, syringomycin, on phosphate metabolism and intracellular ph changes in the yeast rhodotorula pilimanae. syringomycin, at levels between 20 and 60 units per 10(8) cells, caused a cellular efflux of orthophosphate. at 40 and 60 unit per 10(8) cells, the efflux was accompanied by a decrease in polyphosphate and an acidification of the cytoplasm. at low temperatures (5 degrees c) and with 75 units per 10(8) cells, these effec ...19892920179
biotransformation and detoxification of t-2 toxin by soil and freshwater bacteria.bacterial communities isolated from 17 of 20 samples of soils and waters with widely diverse geographical origins utilized t-2 toxin as a sole source of carbon and energy for growth. these isolates readily detoxified t-2 toxin as assessed by a rhodotorula rubra bioassay. the major degradation pathway of t-2 toxin in the majority of isolates involved side chain cleavage of acetyl moieties to produce ht-2 toxin and t-2 triol. a minor degradation pathway of t-2 toxin that involved conversion to neo ...19892705769
incidence of yeasts in pregnant and non-pregnant women in nigeria.the yeast flora of vagina and associated sites (cervix, urethra, perineum and rectum) in 224 pregnant and 106 non-pregnant nigerian women was investigated. a variety of yeasts represented by 12 species were isolated. candida albicans was the most frequently isolated species in both groups, accounting for 63.6% of the isolates from pregnant women and 44.8% of the isolates from non-pregnant women. other species of yeasts infrequently or occasionally encountered were torulopsis glabrata, c. tropica ...19892733724
new spectrum of fungal infections in patients with cancer.we report on 44 cancer patients who had serious infections with unusual fungal pathogens and who were cared for at our cancer center between 1974 and 1986. twelve different fungal species accounted for these infections, including trichosporon beigelii, fusarium species, geotrichum candidum, curvularia species, drechslera species, penicillium species (but not penicillium marneffei), rhodotorula rubra, pseudallescheria boydii, pichia farinosa, torulopsis pintolopesii, saccharomyces cerevisiae, and ...19892749101
yeasts associated with the white shrimp penaeus schmitti, sediment, and water of sepetiba bay, rio de janeiro, brasil.samples were collected in a polluted site with mean fecal coliform counts of the water being 9.0 x 10(2)/100 ml and less polluted areas including commercial fishing areas with fecal coliforms typically below 2.0 x 10(1)/100 ml. yeast populations from the polluted site were 4.6 x 10(2) to 9.2 x 10(3)/g of shrimp intestine, 1.0 x 10(1) to 2.4 x 10(2)/g of sediment and 4.0 x 10(-1) to 4.6 x 10(1)/ml of water, and in the less polluted site 3.0 x 10(1) to 2.3 x 10(2)/g of intestines and 3.0 x 10(-1) ...19892750315
pseudoepidemic of rhodotorula rubra in patients undergoing fiberoptic bronchoscopy.between march and june 1988, rhodotorula rubra was isolated from the bronchial washings of 30 of 56 (54%) patients undergoing bronchoscopy at a north carolina community hospital. pulmonary disease consistent with invasive fungal pneumonia was not apparent for any patient. repeat sputum cultures were performed on 11 patients, none of whom were positive for r rubra. investigation revealed fungal contamination of two brushes used to clean the bronchoscope channels and one positive sample of the tub ...19892584679
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