| isolation, structure determination, and antiaging effects of 2,3-pentanediol from endophytic fungus of curcuma amada and docking studies. | an endophytic fungus was isolated from the rhizomes of curcuma amada (zingiberaceae), which was identified as fusarium oxysporum on the basis of its morphological and molecular characters. chromatographic separation and spectroscopic analysis of the fungal metabolite (chloroform extract) led to the identification of one pure compound having molecular formula c5h12o2, i.e., 2,3-pentanediol (1). activity analysis of compound 1 demonstrated improved antiaging (antioxidant, thermotolerance) properti ... | 2014 | 24515569 |
| functional analysis of chimeric lysin motif domain receptors mediating nod factor-induced defense signaling in arabidopsis thaliana and chitin-induced nodulation signaling in lotus japonicus. | the expression of chimeric receptors in plants is a way to activate specific signaling pathways by corresponding signal molecules. defense signaling induced by chitin from pathogens and nodulation signaling of legumes induced by rhizobial nod factors (nfs) depend on receptors with extracellular lysin motif (lysm) domains. here, we constructed chimeras by replacing the ectodomain of chitin elicitor receptor kinase 1 (atcerk1) of arabidopsis thaliana with ectodomains of nf receptors of lotus japon ... | 2014 | 24506212 |
| induced defense-related proteins in soybean (glycine max l. merrill) plants by carnobacterium sp. sj-5 upon challenge inoculation of fusarium oxysporum. | the aim of the present study was to analyze induced expression of defense-related proteins in the soybean plants by rhizobacterial stain carnobacterium sp. sj-5 upon challenge inoculation with fusarium oxysporum. determination of the enzymatic activity of the different defense-related enzymes, phenylalanine ammonia lyase (pal), lipoxygenase (lox), peroxidase (pod) and polyphenol oxidase (ppo) was performed in the major parts of glycine max l. merrill using spectrophotometric method. native-polya ... | 2014 | 24504695 |
| functional characterization of the gene fooch1 encoding a putative α-1,6-mannosyltransferase in fusarium oxysporum f. sp. cubense. | fusarium oxysporum f. sp. cubense (foc) is the causal agent of banana fusarium wilt and has become one of the most destructive pathogens threatening the banana production worldwide. however, few genes related to morphogenesis and pathogenicity of this fungal pathogen have been functionally characterized. in this study, we identified and characterized the disrupted gene in a t-dna insertional mutant (l953) of foc with significantly reduced virulence on banana plants. the gene disrupted by t-dna i ... | 2014 | 24503549 |
| field resistance to fusarium oxysporum and verticillium dahliae in transgenic cotton expressing the plant defensin nad1. | the plant defensin nad1, from nicotiana alata, has potent antifungal activity against a range of filamentous fungi including the two important cotton pathogens, fusarium oxysporum f. sp. vasinfectum (fov) and verticillium dahliae. transgenic cotton plants expressing nad1 were produced and plants from three events were selected for further characterization. homozygous plants were assessed in greenhouse bioassays for resistance to fov. one line (d1) was selected for field trial testing over three ... | 2014 | 24502957 |
| multilocus analysis using putative fungal effectors to describe a population of fusarium oxysporum from sugar beet. | sugar beet (beta vulgaris) fusarium yellows is caused by fusarium oxysporum f. sp. betae and can lead to significant reductions in root yield, sucrose percentage, juice purity, and storability. f. oxysporum f. sp. betae can be highly variable and many f. oxysporum strains isolated from symptomatic sugar beet are nonpathogenic. identifying pathogenicity factors and their diversity in the f. oxysporum f. sp. betae population could further understanding of how this pathogen causes disease and poten ... | 2014 | 24502207 |
| host-induced post-transcriptional hairpin rna-mediated gene silencing of vital fungal genes confers efficient resistance against fusarium wilt in banana. | fusarium wilt, caused by fusarium oxysporum f. sp. cubense (foc), is among the most destructive diseases of banana (musa spp.). because no credible control measures are available, development of resistant cultivars through genetic engineering is the only option. we investigated whether intron hairpin rna (ihprna)-mediated expression of small interfering rnas (sirnas) targeted against vital fungal genes (velvet and fusarium transcription factor 1) in transgenic banana could achieve effective resi ... | 2014 | 24476152 |
| [cellulolytic activity of fenellia flavipes and fusarium oxysporum strains]. | the production of cellulolytic enzymes by fenellia flavipes and fusarium oxysporum strains in submerged culture with plant residues as carbon source was studied. it was established that the majority of studied strains was able to hydrolyze the filter paper, husk of sunflower seeds, wheat straw and corn stalks. the ability to synthesize enzymes depended on the strain of microscopic fungi, type of substrate and duration of cultivation. as a result two fungal strains were selected: f. flavipes 655 ... | 2014 | 24450186 |
| a new prenylated indole derivative from endophytic actinobacteria streptomyces sp. neau-d50. | a new prenylated indole derivative 3-acetonylidene-7-prenylindolin-2-one (1) was isolated from the endophytic actinobacterium streptomyces sp. neau-d50, together with four known hybrid isoprenoids, 7-isoprenylindole-3-carboxylic acid (2), 3-cyanomethyl-6-prenylindole (3), 6-isoprenylindole-3-carboxylic acid (4) and 7,4'-dihydroxy-5-methoxy-8-(γ,γ-dimethylallyl)-flavanone (5). the structures of these compounds were elucidated on the basis of extensive spectroscopic analysis and comparison with da ... | 2014 | 24443904 |
| fusarium subglutinans: a new eumycetoma agent. | eumycetoma is a chronic subcutaneous mycosis mainly caused by madurella spp. fusarium opportunistic infections in humans are often caused by fusarium solani and fusarium oxysporum. we report a case of eumycetoma by f. subglutinans, diagnosed by clinical aspect and culture, and confirmed by pcr sequencing. the patient was successfully treated with oral itraconazole. to our knowledge, this is the second report of human infection and the first case of mycetoma by fusarium subglutinans. | 2013 | 24432236 |
| salicylic acid and salicylic acid sensitive and insensitive catalases in different genotypes of chickpea against fusarium oxysporum f. sp. ciceri. | differential expression of catalase isozymes in different genotypes of chickpea resistant genotypes- a1, jg-315, jg-11, wr-315, r1-315, vijaya, iccv-15017, gbs-964, gbm-10, and susceptible genotypes- jg-62, mnk, iccv-08321, iccv-08311, kw-104, iccv-08123, icc-4951, icc-11322, icc-08116 for wilt disease caused by fusarium oxysporum. f. sp. ciceri (foc) was analyzed. salicylic acid (sa) and h2o2 concentrations were determined in control as well as in plants infected with f. ciceri and found that t ... | 2013 | 24431522 |
| polyamine metabolism and lipoxygenase activity during fusarium oxysporum f. sp. ricini -castor interaction. | polyamine oxidase and lipoxygenase enzymes are key players for hyper sensitive reaction (hr) during incompatible interaction of host-pathogen. thus, the role of lipoxygenase and polyamines was studied in the wilt pathogen infected and non infected tissues of resistant and susceptible genotypes of castor at 0 days after infection (dai), 5 dai and 10 dai (30 days after sowing). the lipoxygenase (lox) and polyamine oxidase (pao) activities were higher in the incompatible interaction at all the stag ... | 2013 | 24431501 |
| plant defense response against fusarium oxysporum and strategies to develop tolerant genotypes in banana. | soil-borne fungal pathogen, fusarium oxysporum causes major economic losses by inducing necrosis and wilting symptoms in many crop plants. management of fusarium wilt is achieved mainly by the use of chemical fungicides which affect the soil health and their efficiency is often limited by pathogenic variability. hence understanding the nature of interaction between pathogen and host may help to select and improve better cultivars. current research evidences highlight the role of oxidative burst ... | 2014 | 24420701 |
| the fusarium oxysporum gnt2, encoding a putative n-acetylglucosamine transferase, is involved in cell wall architecture and virulence. | with the aim to decipher the molecular dialogue and cross talk between fusarium oxysporum f.sp. lycopersci and its host during infection and to understand the molecular bases that govern fungal pathogenicity, we analysed genes presumably encoding n-acetylglucosaminyl transferases, involved in glycosylation of glycoproteins, glycolipids, proteoglycans or small molecule acceptors in other microorganisms. in silico analysis revealed the existence of seven putative n-glycosyl transferase encoding ge ... | 2013 | 24416097 |
| effect of corn steep liquor on lettuce root rot (fusarium oxysporum f.sp. lactucae) in hydroponic cultures. | recent reports indicate that organic fertilisers have a suppressive effect on the pathogens of plants grown under hydroponic systems. furthermore, microorganisms exhibiting antagonistic activity to diseases have been observed in organic hydroponic systems. this study evaluated the effect of corn steep liquor (csl) on controlling lettuce root rot disease [fusarium oxysporum f.sp. lactucae (fol)] in a hydroponic system. the effect of csl and otsuka a (a chemical fertiliser) on the inhibition of fo ... | 2014 | 24403175 |
| strategies utilized by trophically diverse fungal species for pinus sylvestris root colonization. | physiological changes in host plants in response to the broad spectrum of fungal modes of infection are still not well understood. the current study was conducted to better understand the infection of in vitro cultures of pinus sylvestris l. seedlings by three trophically diverse fungal species, fusarium oxysporum e. f. sm. & swingle, trichoderma harzianum rifai and hebeloma crustuliniforme (bull.) quél. biochemical methods and microscopy were utilized to determine (i) which factors (apoplastic ... | 2014 | 24391166 |
| outbreak of fungal endophthalmitis due to fusarium oxysporum following cataract surgery. | outbreak of exogenous fusarium endophthalmitis after cataract surgery was evaluated. twenty patients developed postoperative endophthalmitis. in 19 eyes, pars plana vitrectomy (ppv) was performed, in 14 cases (74 %) with primary intraocular lens explantation. in one case, the ppv was not performed because of poor general condition of the patient. symptoms of endophthalmitis (damaged vision, iritis, tyndallization in anterior chamber, hypopyon) occurred at intervals of 16-79 days (mean 31.3 days) ... | 2014 | 24381050 |
| development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of fusarium oxysporum f. sp. cubense tropical race 4 in soil. | fusarium oxysporum f. sp. cubense (foc), the causal agent of fusarium wilt (panama disease), is one of the most devastating diseases of banana (musa spp.). the foc tropical race 4 (tr4) is currently known as a major concern in global banana production. no effective resistance is known in musa to foc, and no effective measures for controlling foc once banana plants have been infected in place. early and accurate detection of foc tr4 is essential to protect banana industry and guide banana plantin ... | 2013 | 24376590 |
| higher bioavailability of organic bound zinc from high zinc-enriched fungi. | the organic forms of trace elements are considered more bioavailable than the inorganic forms. although yeast can enrich metal elements and convert inorganic zinc to organic species, its tolerability and transforming capacity are limited. it would therefore be very interesting to look for higher conversion and accumulation in zinc fungi to obtain organic bound zinc from the natural environment. in this paper, potato dextrose agar (pda) medium containing 800 μg/ml zinc was used for initial screen ... | 2014 | 26255549 |
| control of soil-borne diseases by different composts in potted vegetable crops. | the composting process and the type and nature of wastes and raw materials influence the maturity, quality and suppressiveness of composts. variability in disease suppression also depends on the pathosystem, on soil or substrate type, on chemical-physical conditions, like ph and moisture, and on the microbial component of compost. the aim of the research was to evaluate the suppressiveness of composts, originated from green wastes and/or municipal biowastes, and produced by different composting ... | 2014 | 26084080 |
| phenylpropanoid pathway is potentiated by silicon in the roots of banana plants during the infection process of fusarium oxysporum f. sp. cubense. | fusarium wilt, caused by fusarium oxysporum f. sp. cubense, is a disease that causes large reductions in banana yield worldwide. considering the importance of silicon (si) to potentiate the resistance of several plant species to pathogen infection, this study aimed to investigate, at the histochemical level, whether this element could enhance the production of phenolics on the roots of banana plants in response to f. oxysporum f. sp. cubense infection. plants of cultivar maçã, which is susceptib ... | 2014 | 24350769 |
| risk levels of invasive fusarium oxysporum f. sp. in areas suitable for date palm (phoenix dactylifera) cultivation under various climate change projections. | global climate model outputs involve uncertainties in prediction, which could be reduced by identifying agreements between the output results of different models, covering all assumptions included in each. fusarium oxysporum f.sp. is an invasive pathogen that poses risk to date palm cultivation, among other crops. therefore, in this study, the future distribution of invasive fusarium oxysporum f.sp., confirmed by csiro-mk3.0 (cs) and miroc-h (mr) gcms, was modeled and combined with the future di ... | 2013 | 24340100 |
| mutation of fvs1, encoding a protein with a sterile alpha motif domain, affects asexual reproduction in the fungal plant pathogen fusarium oxysporum. | fusarium oxysporum produces three kinds of asexual spores: microconidia, macroconidia and chlamydospores. we previously analysed expressed sequence tags during vegetative growth and conidiation in f. oxysporum and found 42 genes that were markedly upregulated during conidiation compared to vegetative growth. one of the genes, fvs1, encodes a protein with a sterile alpha motif (sam) domain, which functions in protein-protein interactions that are involved in transcriptional or post-transcriptiona ... | 2014 | 24330129 |
| quantification of fusarium oxysporum in fumigated soils by a newly developed real-time pcr assay to assess the efficacy of fumigants for fusarium wilt disease in strawberry plants. | two soil fumigants, chloropicrin (cp) and dimethyl disulfide (dmds), were used to control fusarium wilt disease (fwd) which caused large economic losses in strawberries. the fumigants were evaluated alone and in combination in a laboratory study and in strawberry greenhouses. | 2014 | 24307246 |
| spot drip application of dimethyl disulfide as a post-plant treatment for the control of plant parasitic nematodes and soilborne pathogens in grape production. | plant parasitic nematodes and soilborne pathogens can reduce the overall productivity in grape production. not all grape growers apply soil fumigants before planting, and there is no single rootstock resistant to all nematode species. the aim of this investigation was to evaluate the effect of dimethyl disulfide (dmds) applied at 112, 224, 448 and 897 kg ha(-1) as a post-plant treatment against soilborne plant parasitic nematodes and pathogens on the grape yield in established grapevines. | 2014 | 24307137 |
| analysis of banana transcriptome and global gene expression profiles in banana roots in response to infection by race 1 and tropical race 4 of fusarium oxysporum f. sp. cubense. | cavendish, the most widely grown banana cultivar, is relatively resistant to race 1 of fusarium oxysporum f. sp. cubense (foc1) which caused widespread panama disease during the first half of the 20th century but is susceptible to tropical race 4 of foc (foc tr4) which is threatening world banana production. the genome of the diploid species musa acuminata which is the ancestor of a majority of triploid banana cultivars has recently been sequenced. availability of banana transcriptomes will be h ... | 2013 | 24304681 |
| trichoderma asperelloides suppresses nitric oxide generation elicited by fusarium oxysporum in arabidopsis roots. | inoculations with saprophytic fungus trichoderma spp. are now extensively used both to promote plant growth and to suppress disease development. the underlying mechanisms for both roles have yet to be fully described so that the use of trichoderma spp. could be optimized. here, we show that trichoderma asperelloides effects include the manipulation of host nitric oxide (no) production. no was rapidly formed in arabidopsis roots in response to the soil-borne necrotrophic pathogen fusarium oxyspor ... | 2014 | 24283937 |
| fusaric acid accelerates the senescence of leaf in banana when infected by fusarium. | fusarium oxysporum f.sp. cubense (foc) is a causal agent of vascular wilt and leaf chlorosis of banana plants. chloroses resulting from foc occur first in the lowest leaves of banana seedlings and gradually progress upward. to investigate the responses of different leaf positions to foc infection, hydroponic experiments with foc inoculation were conducted in a greenhouse. fusarium-infected seedlings exhibited a decrease in net photosynthesis rate, stomatal conductance, and transpiration rate of ... | 2014 | 24282097 |
| enhancement of rutin production in fagopyrum tataricum hairy root cultures with its endophytic fungal elicitors. | tartary buckwheat (fagopyrum tataricum) is a potentially important source of rutin, a natural bioactive flavonoid with antihyperglycemic, antioxidative, antihypertensive, and anti-inflammatory properties. this study examines the effects of endophytic fungi on rutin production in the hairy root cultures of f. tataricum. without obvious changes in the appearance of the hairy roots, the exogenous fungal mycelia elicitors efficiently stimulated the hairy root growth and rutin biosynthesis, and the s ... | 2014 | 24279735 |
| synthesis, characterization, and antifungal activity of novel inulin derivatives with chlorinated benzene. | a group of novel inulin derivatives containing benzene or chlorinated benzene were synthesized by reaction of chloracetyl inulin (cail) with the schiff bases of 4-amino-pyridine, including (2-pyridyl)acetyl inulin chloride (pail), 2-[4-(2-chlorobenzylideneamino)-pyridyl]acetyl inulin chloride (2cpail), 2-[4-(4-chlorobenzylideneamino)-pyridyl]acetyl inulin chloride (4cpail), and 2-[4-(2,4-dichlorobenzylideneamino)-pyridyl]acetyl inulin chloride (2,4dcpail). their antifungal activity against three ... | 2014 | 24274532 |
| proteomic investigation of response to forl infection in tomato roots. | fusarium oxysporum f. sp. radicis-lycopersici (forl) leading to fusarium crown and root rot is considered one of the most destructive tomato soilborne diseases occurring in greenhouse and field crops. in this study, response to forl infection in tomato roots was investigated by differential proteomics in susceptible (monalbo) and resistant (momor) isogenic tomato lines, thus leading to identify 33 proteins whose amount changed depending on the pathogen infection, and/or on the two genotypes. for ... | 2014 | 24262994 |
| characterization of fusarium isolates from asparagus fields in southwestern ontario and influence of soil organic amendments on fusarium crown and root rot. | fusarium crown and root rot (fcrr) of asparagus has a complex etiology with several soilborne fusarium spp. as causal agents. ninety-three fusarium isolates, obtained from plant and soil samples collected from commercial asparagus fields in southwestern ontario with a history of fcrr, were identified as fusarium oxysporum (65.5%), f. proliferatum (18.3%), f. solani (6.4%), f. acuminatum (6.4%), and f. redolens (3.2%) based on morphological or cultural characteristics and polymerase chain reactio ... | 2014 | 24261409 |
| development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of fusarium oxysporum f. sp. niveum in soil. | fusarium wilt caused by fusarium oxysporum f. sp. niveum (fon) is one of the major limiting factors for watermelon production worldwide. rapid and accurate detection of the causal pathogen is the cornerstone of integrated disease management. in this paper, a real-time fluorescence loop-mediated isothermal amplification (realamp) assay was developed for the rapid and quantitative detection of fon in soil. positive products were amplified only from fon isolates and not from any other species or fo ... | 2013 | 24256412 |
| plant growth-promoting activities of streptomyces spp. in sorghum and rice. | five strains of streptomyces (cai-24, cai-121, cai-127, kai-32 and kai-90) were earlier reported by us as biological control agents against fusarium wilt of chickpea caused by fusarium oxysporum f. sp. ciceri (foc). in the present study, the streptomyces were characterized for enzymatic activities, physiological traits and further evaluated in greenhouse and field for their plant growth promotion (pgp) of sorghum and rice. all the streptomyces produced lipase, β-1-3-glucanase and chitinase (exce ... | 2013 | 24255867 |
| optimization of biological synthesis of silver nanoparticles using fusarium oxysporum. | silver nanoparticles are increasingly used in various fields of biotechnology and applications in the medicine. objectives of this study were optimization of production of silver nanoparticles using biotransformations by fusarium oxysporum, and a further study on the location of nanoparticles synthesis in this microorganism. the reaction mixture contained the following ingredients (final concentrations): agno3 (1-10 mm) as the biotransformation substrate, biomass as the biocatalyst, glucose (560 ... | 2013 | 24250635 |
| the role of onion-associated fungi in bulb mite infestation and damage to onion seedlings. | in israel rhizoglyphus robini is considered to be a pest in its own right, even though the mite is usually found in association with fungal pathogens. plant protection recommendations are therefore to treat germinating onions seedlings, clearly a crucial phase in crop production, when mites are discovered. the aim of this study was to determine the role of fungi in bulb mite infestation and damage to germinating onion seedlings. accordingly we (1) evaluated the effect of the mite on onion seedli ... | 2014 | 24242868 |
| combinatorial function of velvet and area in transcriptional regulation of nitrate utilization and secondary metabolism. | velvet is a conserved protein complex that functions as a regulator of fungal development and secondary metabolism. in the soil-inhabiting pathogen fusarium oxysporum, velvet governs mycotoxin production and virulence on plant and mammalian hosts. here we report a previously unrecognized role of the velvet complex in regulation of nitrate metabolism. f. oxysporum mutants lacking vea or laea, two key components of the complex, were impaired in growth on the non-preferred nitrogen sources nitrate ... | 2014 | 24240057 |
| ebr1 genomic expansion and its role in virulence of fusarium species. | genome sequencing of fusarium oxysporum revealed that pathogenic forms of this fungus harbour supernumerary chromosomes with a wide variety of genes, many of which likely encode traits required for pathogenicity or niche specialization. specific transcription factor gene families are expanded on these chromosomes including the ebr1 family (enhanced branching). the significance of the ebr1 family expansion on supernumerary chromosomes and whether ebr1 paralogues are functional is currently unknow ... | 2014 | 24237614 |
| rapid synthesis of silver nanoparticles from fusarium oxysporum by optimizing physicocultural conditions. | synthesis of silver nanoparticles (snps) by fungi is emerging as an important branch of nanotechnology due to its ecofriendly, safe, and cost-effective nature. in order to increase the yield of biosynthesized snps of desired shape and size, it is necessary to control the cultural and physical parameters during the synthesis. we report optimum synthesis of snps on malt extract glucose yeast extract peptone (mgyp) medium at ph 9-11, 40-60°c, and 190.7 lux and in sun light. the salt concentrations, ... | 2013 | 24222751 |
| rohitukine, a chromone alkaloid and a precursor of flavopiridol, is produced by endophytic fungi isolated from dysoxylum binectariferum hook.f and amoora rohituka (roxb).wight & arn. | rohitukine, a chromone alkaloid, has gained considerable international attention in recent years because of its novel semi-synthetic derivative, flavopiridol and p-276-00. both these molecules are in advanced stages of clinical development and trial for cancer treatment. recently, flavopiridol was approved as an orphan drug for treatment of chronic lymphocytic leukemia cancer. the natural occurrence of rohitukine is restricted to only four plant species, amoora rohituka and dysoxylum binectarife ... | 2014 | 24215673 |
| interactions between the entomopathogenic nematode heterorhabditis sonorensis (nematoda: heterorhabditidae) and the saprobic fungus fusarium oxysporum (ascomycota: hypocreales). | in this study, we assessed the effect of the saprobic fungus, fusarium oxysporum (ascomycota: hypocreales) on the fitness of the entomopathogenic nematode heterorhabditis sonorensis (caborca strain). sand column assays were considered to evaluate the effect of fungal mycelia on infective juvenile (ij) movement and host access. additionally, we investigated the effect of fungal spores on the nematodes' ability to search for a host, its virulence, penetration efficiency and reproduction. three app ... | 2014 | 24211424 |
| synthesis and antifungal properties of (4-tolyloxy)-pyrimidyl-α-aminophosphonates chitosan derivatives. | a novel class of α-aminophosphonate chitosan derivatives was investigated. these chitosan derivatives consist of (4-tolyloxy)-pyrimidyl-dimethyl-α-amino-phosphonate chitosan (α-atpmcs) and (4-tolyloxy)-pyrimidyl-diethyl-α-aminophosphonate chitosan (α-atpecs). their structures were well defined. antifungal activity of them against some crop-threatening pathogenic fungi was tested in vitro. the derivatives were found to have a broad-spectrum antifungal activity that was obviously enhanced compared ... | 2014 | 24183805 |
| routine mapping of fusarium wilt resistance in bc(1) populations of arabidopsis thaliana. | susceptibility to fusarium wilt disease varies among wild accessions of arabidopsis thaliana. six resistance to fusarium oxysporum (rfo) quantitative trait loci (qtls) controlling the resistance of accession columbia-0 (col-0) and susceptibility of taynuilt-0 to fusarium oxysporum forma specialis matthioli (fom) are detected in a recombinant population derived from a single backcross of the f1 hybrid (bc1). in particular, the rfo1 qtl appears to interact with three other loci, rfo2, rfo4 and rfo ... | 2013 | 24172069 |
| biofilm formation by fusarium oxysporum f. sp. cucumerinum and susceptibility to environmental stress. | to the authors' knowledge, most studies on biofilm formation have focused on bacteria and yeasts. so far, biofilm formation by fungal plant pathogen has not been reported. in this study, the biofilm-forming capacity of fusarium oxysporum f. sp. cucumerinum was evaluated. for biofilm quantification, a colorimetric 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2h-tetrazolium-hydroxide (xtt) reduction assay was used to observe metabolic activity. fluorescence and confocal scann ... | 2014 | 24164057 |
| antifungal activity of microgramma vacciniifolia rhizome lectin on genetically distinct fusarium oxysporum f. sp. lycopersici races. | fusarium oxysporum f. sp. lycopersici races 1, 2, and 3 deteriorate tomato crops since they cause a vascular wilt. lectins are carbohydrate-binding proteins with hemagglutinating and antifungal activities. this work reports that microgramma vacciniifolia rhizome lectin (mvrl) inhibits f. oxysporum f. sp. lycopersici race 3 growth (61 %) more intensely than of races 1 (55 %) and 2 (45 %). the hemagglutinating activity of mvrl was inhibited by glycoprotein preparations from mycelia of races 1, 2, ... | 2014 | 24142386 |
| insights from the draft genome of paenibacillus lentimorbus nrrl b-30488, a promising plant growth promoting bacterium. | paenibacillus lentimorbus nrrl b-30488, a plant growth-promoting bacterium was isolated from sahiwal cow's milk. the strain shows antagonism against phytopathogens, fusarium oxysporum f. sp. ciceri and alternaria solani. its genome contains gene clusters involved in nonribosomal synthesis of secondary metabolites involved in antimicrobial activities. the genome sequence of p. lentimorbus nrrl b-30488 provides the genetic basis for application of this bacterial strain in plant growth promotion, p ... | 2013 | 24140292 |
| protein extraction and gel-based separation methods to analyze responses to pathogens in carnation (dianthus caryophyllus l). | we are currently using a 2-de-based proteomics approach to study plant responses to pathogenic fungi by using the carnation (dianthus caryophyllus l)-fusarium oxysporum f. sp. dianthi pathosystem. it is clear that the protocols for the first stages of a standard proteomics workflow must be optimized to each biological system and objectives of the research. the optimization procedure for the extraction and separation of proteins by 1-de and 2-de in the indicated system is reported. this strategy ... | 2014 | 24136548 |
| long noncoding rnas responsive to fusarium oxysporum infection in arabidopsis thaliana. | short noncoding rnas have been demonstrated to play important roles in regulation of gene expression and stress responses, but the repertoire and functions of long noncoding rnas (lncrnas) remain largely unexplored, particularly in plants. to explore the role of lncrnas in disease resistance, we used a strand-specific rna-sequencing approach to identify lncrnas responsive to fusarium oxysporum infection in arabidopsis thaliana. antisense transcription was found in c. 20% of the annotated a. thal ... | 2014 | 24117540 |
| crude extract of fusarium oxysporum induces apoptosis and structural alterations in the skin of healthy rats. | we evaluate the biological and physicochemical effects of a fusarium oxysporum crude extract (ce) on the skin of healthy rats. the ce is topically applied and subsequently the skin is collected after 3, 6, 12, and 24 h. the samples are analyzed by fourier transform infrared photoacoustic spectroscopy (ftir-pas) and histomorphometric analysis. terminal dutp nick end labeling (tunel assay) is performed to detect both the cells in apoptosis and proliferation. there is a thickening of the epidermis ... | 2013 | 24077666 |
| integrated management strategies for tomato fusarium wilt. | fusarium wilt is caused by the fungal pathogens, fusarium oxysporum or fusarium solani. it is a devastating disease that affects many important food and vegetable crops and a major source of loss to farmers worldwide. initial strategies developed to combat this devastating plant disease include the use of cultural, physical and chemical control. none of these strategies have been able to give the best results of completely ameliorating the situation except for the cultural method which is mainly ... | 2013 | 24077535 |
| synthesis and bioactivities of novel thioether/sulfone derivatives containing 1,2,3-thiadiazole and 1,3,4-oxadiazole/thiadiazole moiety. | a series of new thioether/sulfone compounds containing 1,2,3-thiadiazole and 1,3,4-oxadiazole/1,3,4-thiadiazole moiety were synthesized, the structures of all products were confirmed by ir, (1)h nmr, (13)c nmr, and element analysis. preliminary antifungal activity test showed that compound 8a exhibited moderate antifungal activity against fusarium oxysporum at 50μg/ml. preliminary antiviral activity results showed that compounds 7a, 7c, 7d, 8a, and 9a displayed high antiviral activity against to ... | 2013 | 24070781 |
| proteomic analysis of fusarium oxysporum f. sp. cubense tropical race 4-inoculated response to fusarium wilts in the banana root cells. | fusarium wilt of banana is one of the most destructive diseases in the world. this disease has caused heavy losses in major banana production areas. except for molecular breeding methods based on plant defense mechanisms, effective methods to control the disease are still lacking. dynamic changes in defense mechanisms between susceptible, moderately resistant, and highly resistant banana and fusarium oxysporum f. sp. cubense tropical race 4 (foc4) at the protein level remain unknown. this resear ... | 2013 | 24070062 |
| isolation, purification and characterization of vinblastine and vincristine from endophytic fungus fusarium oxysporum isolated from catharanthus roseus. | endophytic fungi reside in a symbiotic fashion inside their host plants, mimic their chemistry and interestingly, produce the same natural products as their hosts and are thus being screened for the production of valuable compounds like taxol, camptothecin, podophyllotoxin, etc. vinblastine and vincristine are excellent anti-cancer drugs but their current production using plants is non-abundant and expensive. in order to make these drugs readily available to the patients at affordable prices, we ... | 2013 | 24066024 |
| spectroscopic and biological approach in the characterization of a novel 14-membered [n4] macrocyclic ligand and its palladium(ii), platinum(ii), ruthenium(iii) and iridium(iii) complexes. | a novel, tetradentate nitrogen donor [n4] macrocyclic ligand, i.e. 3,5,14,16-tetramethyl-2,6,13,17-tetraazatricyclo[12,0,0(7-12)] cosa-1(22),2,5,7,9,11,13,16,18,20-decaene(l), has been synthesized and characterized by elemental analyses, ir, mass, and (1)h nmr spectral studies. complexes of pd(ii), pt(ii), ru(iii) and ir(iii) have been prepared and characterized by elemental analyses, molar conductance measurements, magnetic susceptibility measurements, ir, mass, electronic spectral and thermal ... | 2014 | 24051297 |
| increased incidence of invasive fusariosis with cutaneous portal of entry, brazil. | invasive fusariosis (if) is an infection with fusarium spp. fungi that primarily affects patients with hematologic malignancies and hematopoietic cell transplant recipients. a cutaneous portal of entry is occasionally reported. we reviewed all cases of if in brazil during 2000-2010, divided into 2 periods: 2000-2005 (period 1) and 2006-2010 (period 2). we calculated incidence rates of if and of superficial infections with fusarium spp. fungi identified in patients at a dermatology outpatient uni ... | 2013 | 24050318 |
| fusarium keratitis in brazil: genotyping, in vitro susceptibilities, and clinical outcomes. | the purpose of this paper is to describe clinical characteristics and determine correlations between clinical outcomes and antifungal susceptibility among molecularly characterized ocular fusarium isolates in brazil. | 2013 | 24039389 |
| surveillance of bacterial and fungal infections in the postoperative period following liver transplantation: a series from 2005-2011. | orthotopic liver transplantation (olt) is a life-saving procedure for the treatment of many end-stage diseases, but infectious and acute rejection episodes remain major causes of morbidity and mortality. bacterial and fungal infections can be due to intra-abdominal, biliary, respiratory, urinary, wound, central venous catheters (cvc) or unknown sources. using the computerized database of our microbiology laboratory, we analyzed all the bacterial and fungal infections in the first three months fo ... | 2013 | 24034031 |
| synthesis and bioactivity evaluation of novel arylimines containing a 3-aminoethyl-2-[(p-trifluoromethoxy)anilino]-4(3h)-quinazolinone moiety. | twenty-seven novel (e)-3-[2-arylideneaminoethyl]-2-[4-(trifluoromethoxy)anilino]-4(3h)-quinazolinone derivatives were synthesized by reacting various aromatic aldehydes with intermediate 6. the target compounds were characterized by (1)h nmr, (3)c nmr, ir, and elemental analysis. bioassay results revealed that some of the compounds have strong antifungal activities against six fungi ( gibberella zeae , fusarium oxysporum , clematis mandshurica , paralepetopsis sasakii , phytophthora infestans , ... | 2013 | 24028303 |
| fusarium pathogenomics. | fusarium is a genus of filamentous fungi that contains many agronomically important plant pathogens, mycotoxin producers, and opportunistic human pathogens. comparative analyses have revealed that the fusarium genome is compartmentalized into regions responsible for primary metabolism and reproduction (core genome), and pathogen virulence, host specialization, and possibly other functions (adaptive genome). genes involved in virulence and host specialization are located on pathogenicity chromoso ... | 2013 | 24024636 |
| [bletilla striata pseudobulb rot pathogen identification and its biological characteristic]. | to identify the pathogen of bletilla striata pseudobulb rot and provide foundation for formulation effective control measures. | 2013 | 24010310 |
| molecular detection and genotyping of fusarium oxysporum f. sp. psidii isolates from different agro-ecological regions of india. | twenty one isolates of fusarium oxysporum f. sp. psidii (fop), causing a vascular wilt in guava (psidium guajava l.), were collected from different agro-ecological regions of india. the pathogenicity test was performed in guava seedlings, where the fop isolates were found to be highly pathogenic. all 21 isolates were confirmed as f. oxysporum f. sp. psidii by a newly developed, species-specific primer against the conserved regions of 28s rdna and the intergenic spacer region. rapd and pcr-rflp w ... | 2013 | 23990290 |
| cross-talk interactions of exogenous nitric oxide and sucrose modulates phenylpropanoid metabolism in yellow lupine embryo axes infected with fusarium oxysporum. | the aim of the study was to examine cross-talk of exogenous nitric oxide (no) and sucrose in the mechanisms of synthesis and accumulation of isoflavonoids in embryo axes of lupinus luteus l. cv. juno. it was verified whether the interaction of these molecules can modulate the defense response of axes to infection and development of the pathogenic fungus fusarium oxysporum f. sp. lupini. sucrose alone strongly stimulated a high level of genistein glucoside in axes pretreated with exogenous nitric ... | 2013 | 23987816 |
| use of a passive bioreactor to reduce water-borne plant pathogens, nitrate, and sulfate in greenhouse effluent. | the goal of this study was to evaluate the use of passive bioreactors to reduce water-borne plant pathogens (pythium ultimum and fusarium oxysporum) and nutrient load (no(-) 3 and so(2-) 4) in greenhouse effluent. sterilized and unsterilized passive bioreactors filled with a reactive mixture of organic carbon material were used in three replicates. after a startup period of 2 (sterilized) or 5 (unsterilized) weeks, the bioreactor units received for 14 weeks a reconstituted commercial greenhouse ... | 2013 | 23947714 |
| effects of electrokinetic operation mode on removal of polycyclic aromatic hydrocarbons (pahs), and the indigenous fungal community in pah-contaminated soil. | electrokinetic remediation is an emerging physical remediation technology for the removal of heavy metals and organic chemicals from contaminated soil. we set up a soil chamber (24 × 12 × 8 cm) with two stainless steel electrodes (12 × 0.5 cm), and a constant voltage gradient of 1.0 v cm(-1) or 2.0 v cm(-1) was applied to study the effects of unidirectional and altered directional electric field operation modes on the moisture content and ph, the removal rate of pahs, and the abundance and diver ... | 2013 | 23947706 |
| birth, death and horizontal transfer of the fumonisin biosynthetic gene cluster during the evolutionary diversification of fusarium. | fumonisins are a family of carcinogenic secondary metabolites produced by members of the fusarium fujikuroi species complex (ffsc) and rare strains of fusarium oxysporum. in fusarium, fumonisin biosynthetic genes (fum) are clustered, and the cluster is uniform in gene organization. here, sequence analyses indicated that the cluster exists in five different genomic contexts, defining five cluster types. in fum gene genealogies, evolutionary relationships between fusaria with different cluster typ ... | 2013 | 23937442 |
| rhizobacterium-mediated growth promotion and expression of stress enzymes in glycine max l. merrill against fusarium wilt upon challenge inoculation. | wilt disease of soybean caused by a very common soil-borne fungus, fusarium oxysporum is one of the most destructive diseases of the crop. the aim of the present study was to characterize plant growth-promotion activities and induced resistance of a rhizobacterial strain for the soybean plant against f. oxysporum. rhizobacterium strain sj-5 exhibited plant growth-promotion characteristics and antagonistic activity against the test pathogen on dual plate assay. it was identified as a carnobacteri ... | 2014 | 23933805 |
| contamination of bananas with beauvericin and fusaric acid produced by fusarium oxysporum f. sp. cubense. | fusarium wilt, caused by the fungal pathogen fusarium oxysporum f. sp. cubense (foc), is one of the most destructive diseases of banana. toxins produced by foc have been proposed to play an important role during the pathogenic process. the objectives of this study were to investigate the contamination of banana with toxins produced by foc, and to elucidate their role in pathogenesis. | 2013 | 23922960 |
| [effects of nitrogen application rate on faba bean fusarium wilt and rhizospheric microbial metabolic functional diversity]. | a field plot experiment was conducted to study the effects of different nitrogen (n) application rates on the microbial functional diversity in faba bean rhizosphere and the relationships between the microbial functional diversity and the occurrence of faba bean fusarium wilt. four nitrogen application rates were installed, i. e. , n0(0 kg hm-2 , n1 (56. 25 kg hm-2) , n2(112. 5 kg hm-2), and n3 (168.75 kg hm-2), and biolog microbial analysis system was applied to study the damage of faba bean fu ... | 2013 | 23898671 |
| biochemical markers assisted screening of fusarium wilt resistant musa paradisiaca (l.) cv. puttabale micropropagated clones. | an efficient protocol was standardized for screening of panama wilt resistant musa paradisiaca cv. puttabale clones, an endemic cultivar of karnataka, india. the synergistic effect of 6-benzyleaminopurine (2 to 6 mg/l) and thidiazuron (0.1 to 0.5 mg/l) on ms medium provoked multiple shoot induction from the excised meristem. an average of 30.10 +/- 5.95 shoots was produced per propagule at 4 mg/l 6-benzyleaminopurine and 0.3 mg/l thidiazuron concentrations. elongation of shoots observed on 5 mg/ ... | 2013 | 23898552 |
| characterization of chlordecone-tolerant fungal populations isolated from long-term polluted tropical volcanic soil in the french west indies. | the insecticide chlordecone is a contaminant found in most of the banana plantations in the french west indies. this study aims to search for fungal populations able to grow on it. an andosol heavily contaminated with chlordecone, perfused for 1 year in a soil-charcoal system, was used to conduct enrichment cultures. a total of 103 fungal strains able to grow on chlordecone-mineral salt medium were isolated, purified, and deposited in the miae collection (microorganismes d'intérêt agro-environne ... | 2014 | 23872892 |
| slow sand filters effectively reduce phytophthora after a pathogen switch from fusarium and a simulated pump failure. | slow sand filtration has been shown to effectively reduce phytophthora zoospores in irrigation water. this experiment tested the reduction of phytophthora colony forming units (cfus) by slow sand filtration systems after switching the pathogen contaminating plant leachate from fusarium to phytophthora and the resilience of the system to a short period without water, as might be caused by a pump failure. the slow sand filtration system greatly reduced phytophthora cfus and transmission after swit ... | 2013 | 23866129 |
| plant defensin type 1 (pdf1): protein promiscuity and expression variation within the arabidopsis genus shed light on zinc tolerance acquisition in arabidopsis halleri. | plant defensins are recognized for their antifungal properties. however, a few type 1 defensins (pdf1s) were identified for their cellular zinc (zn) tolerance properties after a study of the metal extremophile arabidopsis halleri. in order to investigate whether different paralogues would display specialized functions, the a. halleri pdf1 family was characterized at the functional and genomic levels. eleven pdf1s were isolated from a. halleri. their ability to provide zn tolerance in yeast cells ... | 2013 | 23865749 |
| comparative mapping of raphanus sativus genome using brassica markers and quantitative trait loci analysis for the fusarium wilt resistance trait. | fusarium wilt (fw), caused by the soil-borne fungal pathogen fusarium oxysporum is a serious disease in cruciferous plants, including the radish (raphanus sativus). to identify quantitative trait loci (qtl) or gene(s) conferring resistance to fw, we constructed a genetic map of r. sativus using an f2 mapping population derived by crossing the inbred lines '835' (susceptible) and 'b2' (resistant). a total of 220 markers distributed in 9 linkage groups (lgs) were mapped in the raphanus genome, cov ... | 2013 | 23864230 |
| in vitro activity of miltefosine as a single agent and in combination with voriconazole or posaconazole against uncommon filamentous fungal pathogens. | antifungal treatment of uncommon filamentous fungal infections is problematic. this study determined the in vitro susceptibility of miltefosine, as a single agent and in combination with posaconazole or voriconazole, against these pathogens. | 2013 | 23861311 |
| proteomic identification of potential target proteins regulated by the scf(f) (bp1) -mediated proteolysis pathway in fusarium oxysporum. | f-box proteins function in the recruitment of proteins for scf ubiquitination and proteasome degradation. here, we studied the role of fbp1, a nonessential f-box protein of the tomato pathogen fusarium oxysporum f. sp. lycopersici. the δfbp1 mutant showed a significant delay in the production of wilt symptoms on tomato plants and was impaired in invasive growth on cellophane membranes and on living plant tissue. to search for target proteins recruited by fbp1, a combination of sodium dodecylsulp ... | 2013 | 23855991 |
| identification of immunity related genes to study the physalis peruviana--fusarium oxysporum pathosystem. | the cape gooseberry (physalisperuviana l) is an andean exotic fruit with high nutritional value and appealing medicinal properties. however, its cultivation faces important phytosanitary problems mainly due to pathogens like fusarium oxysporum, cercosporaphysalidis and alternaria spp. here we used the cape gooseberry foliar transcriptome to search for proteins that encode conserved domains related to plant immunity including: nbs (nucleotide binding site), cc (coiled-coil), tir (toll/interleukin ... | 2013 | 23844210 |
| fusaric acid induction of programmed cell death modulated through nitric oxide signalling in tobacco suspension cells. | fusaric acid (fa) is a nonhost-selective toxin mainly produced by fusarium oxysporum, the causal agent of plant wilt diseases. we demonstrate that fa can induce programmed cell death (pcd) in tobacco suspension cells and the fa-induced pcd is modulated by nitric oxide (no) signalling. cells undergoing cell death induced by fa treatment exhibited typical characteristics of pcd including cytoplasmic shrinkage, chromatin condensation, dna fragmentation, membrane plasmolysis, and formation of small ... | 2013 | 23838885 |
| analysis of hairpin rna transgene-induced gene silencing in fusarium oxysporum. | hairpin rna (hprna) transgenes can be effective at inducing rna silencing and have been exploited as a powerful tool for gene function analysis in many organisms. however, in fungi, expression of hairpin rna transcripts can induce post-transcriptional gene silencing, but in some species can also lead to transcriptional gene silencing, suggesting a more complex interplay of the two pathways at least in some fungi. because many fungal species are important pathogens, rna silencing is a powerful te ... | 2013 | 23819794 |
| disseminated fusariosis secondary to neuroblastoma with fatal outcome. | disseminated fusariosis is an uncommon clinical condition in immunocompromised patients. we report a fatal case of disseminated fusariosis secondary to neuroblastoma in a male patient, 15 years old, who underwent a bone marrow transplant. the patient was admitted to the pediatric intensive care unit (picu) of a public hospital in recife-pe, brazil, presenting bone marrow aplasia, severe leukopenia, and thrombocytopenia. after 15 days, the patient developed right knee effusion. synovial fluid and ... | 2013 | 23813153 |
| diversity in receptor-like kinase genes is a major determinant of quantitative resistance to fusarium oxysporum f.sp. matthioli. | resistance to wilt fungus fusarium oxysporum f.sp. matthioli (fom) is a polygenic trait in arabidopsis thaliana. rfo3 is one of six quantitative trait loci accounting for the complete resistance of accession columbia-0 (col-0) and susceptibility of accession taynuilt-0 (ty-0). we find that col-0 and ty-0 alleles of rfo3 are representative of two common variants in wild arabidopsis accessions, that resistance and susceptibility to fom are ancestral features of the two variants and that resistance ... | 2013 | 23790083 |
| modified primers for the identification of nonpathogenic fusarium oxysporum isolates that have biological control potential against fusarium wilt of cucumber in taiwan. | previous investigations demonstrated that fusarium oxysporum (fo), which is not pathogenic to cucumbers, could serve as a biological control agent for managing fusarium wilt of cucumber caused by fo f. sp. cucumerinum (foc) in taiwan. however, thus far it has not been possible to separate the populations of pathogenic fo from the nonpathogenic isolates that have biological control potential through their morphological characteristics. although these two populations can be distinguished from one ... | 2013 | 23762289 |
| fusariotoxins in asparagus - their biosynthesis and migration. | asparagus is often infected by fungi of the fusarium genus, a causal agent of crown and root rot, which decreases the quantity and quality of spears. fusarium oxysporum and fusarium proliferatum are the most severe asparagus pathogens, well known as mycotoxin producers, mainly fumonisins and moniliformin. the present study was undertaken to estimate fumonisin b1, moniliformin and ergosterol concentrations in asparagus tissue. moreover, the possibility of toxin transport to the edible asparagus p ... | 2013 | 23756238 |
| phenazine antibiotic production and antifungal activity are regulated by multiple quorum-sensing systems in pseudomonas chlororaphis subsp. aurantiaca stfrb508. | a number of gram-negative bacteria have a quorum-sensing system and produce the n-acylhomoserine lactone (ahl) as a signal molecule. pseudomonas chlororaphis subsp. aurantiaca stfrb508 produces one of the phenazine derivatives, phenazine-1-carboxylic acid (pca). whole-genome sequencing of stfrb508 revealed the presence of two sets of ahl-synthase and ahl-receptor gene, phzir and aurir. the mutation of phzi drastically decreased pca production, but the mutation of auri did not affect pca producti ... | 2013 | 23727350 |
| [fusariosis diagnosed in the laboratory of an uh in tunisia: epidemiological, clinical and mycological study]. | the genus fusarium, initially known for its important agro-economic impact, is more and more often implicated in human pathology. in fact, multiples allergic, toxic and infectious manifestations are more reported in immunocompetent and immunocompromised hosts. the objective of our study was to analyse the epidemiological, mycological and clinical features of fusariosis reported in our chu. eighty-seven cases of fusarium infections were collected: 34 cases of onychomycosis (39%), 26 cases of inte ... | 2013 | 23725904 |
| lipolytic system of the tomato pathogen fusarium oxysporum f. sp. lycopersici. | the lipolytic profile of fusarium oxysporum f. sp lycopersici was studied by in silico search and biochemical enzyme activity analyses. twenty-five structural secreted lipases were predicted based on the conserved pentapeptide gly-x-ser-x-gly-, characteristic of fungal lipases, and secretion signal sequences. moreover, a predicted lipase regulatory gene was identified in addition to the previously characterized ctf1. the transcription profile of thirteen lipase genes during tomato plant coloniza ... | 2013 | 23718123 |
| arabidopsis thaliana resistance to fusarium oxysporum 2 implicates tyrosine-sulfated peptide signaling in susceptibility and resistance to root infection. | in the plant arabidopsis thaliana, multiple quantitative trait loci (qtls), including rfo2, account for the strong resistance of accession columbia-0 (col-0) and relative susceptibility of taynuilt-0 (ty-0) to the vascular wilt fungus fusarium oxysporum forma specialis matthioli. we find that rfo2 corresponds to diversity in receptor-like protein (rlp) genes. in col-0, there is a tandem pair of rlp genes: rfo2/at1g17250 confers resistance while rlp2 does not. in ty-0, the highly diverged rfo2 lo ... | 2013 | 23717215 |
| hyperkeratotic warty skin lesion of foot caused by fusarium oxysporum. | fusarium species are common soil-inhabiting organisms and plant pathogens. human infections are usually precipitated by local or systemic predisposing factors, and disseminated infection is associated with impaired immune responses. skin infections caused by fusarium spp. include keratitis, onychomycosis, mycetoma, painful discrete erythematous nodules. hyperkeratotic skin lesions caused by fusarium spp. are, however, rarely reported. we report a case of hyperkeratotic verrucous warty skin lesio ... | 2013 | 23716829 |
| epi-trichosetin, a new undecaprenyl pyrophosphate synthase inhibitor, produced by fusarium oxysporum fki-4553. | a new compound, designated epi-trichosetin (1), was isolated along with the known compound trichosetin (2) from the culture broth of fusarium oxysporum fki-4553 by solvent extraction, silica gel column chromatography and reversed-phase hplc. the structure of 1 was elucidated by comparing various spectral data with those of 2, revealing that 1 was a stereoisomer of 2. compounds 1 and 2 inhibited the undecaprenyl pyrophosphate synthase activity of staphylococcus aureus with ic50 values of 83 and 3 ... | 2013 | 23715038 |
| systemic acquired resistance in cavendish banana induced by infection with an incompatible strain of fusarium oxysporum f. sp. cubense. | fusarium wilt of banana is caused by the soil-borne fungus fusarium oxysporum f. sp. cubense (foc). the fact that there are no economically viable biological, chemical, or cultural measures of controlling the disease in an infected field leads to search for alternative strategies involving activation of the plant's innate defense system. the mechanisms underlying systemic acquired resistance (sar) are much less understood in monocots than in dicots. since systemic protection of plants by attenua ... | 2013 | 23702248 |
| effectiveness of dyes removal by mixed fungal cultures and toxicity of their metabolites. | decolorization of brilliant green (0.06 g/l), evans blue (0.15 g/l), and their mixture (total concentration 0.08 g/l, proportion 1:1 w/w) by fungi was studied. fungal strains [pleurotus ostreatus (bwph), gloeophyllum odoratum (dca), and fusarium oxysporum (g1)] were used separately and as a mixture of them. zootoxicity (daphnia magna) and phytotoxicity (lemna minor) changes were estimated after the end of experiment. mixtures of fungal strains were less effective in decolorization process than t ... | 2013 | 23687394 |
| coniochaetones e-i, new 4h-chromen-4-one derivatives from the cordyceps-colonizing fungus fimetariella sp. | five new 4h-chromen-4-one derivatives coniochaetones e-i (1-5), along with the known compounds coniochaetones b (6) and a (7) have been isolated from solid cultures of the cordyceps-colonizing fungus fimetariella sp. their structures were elucidated primarily by nmr spectroscopy and the absolute configurations of compounds 1-3 were assigned using the modified mosher's method. compound 4 showed weak cytotoxic activity against hela cells with ic50 values of 72.8 μm. the co-isolated known compound ... | 2013 | 23685047 |
| molecular cloning and expression of five glutathione s-transferase (gst) genes from banana (musa acuminata l. aaa group, cv. cavendish). | three tau class magsts responded to abiotic stress, magstf1 and magstl1 responded to signaling molecules, they may play an important role in the growth of banana plantlet. glutathione s-transferases (gst) are multifunctional detoxification enzymes that participate in a variety of cellular processes, including stress responses. in this study, we report the molecular characteristics of five gst genes (magstu1, magstu2, magstu3, magstf1 and magstl1) cloned from banana (musa acuminate l. aaa group, ... | 2013 | 23652818 |
| maldi-fticr ms imaging as a powerful tool to identify paenibacillus antibiotics involved in the inhibition of plant pathogens. | nowadays, microorganisms are more and more often used as biocontrol agents for crop protection against diseases. among them, bacteria of bacillus and paenibacillus genders are already used as commercial biocontrol agents. their mode of action is supposed to be related to their production of antibiotics, such as cyclic lipopeptides, which exhibit great antimicrobial activities. we chose to work with a paenibacillus polymyxa strain (pp56) very resistant to various microorganisms. the bacteria were ... | 2013 | 23636858 |
| the endophytic strain fusarium oxysporum fo47: a good candidate for priming the defense responses in tomato roots. | the protective fusarium oxysporum strain fo47 is effective in controlling fusarium wilt in tomato. previous studies have demonstrated the role of direct antagonism and involvement of induced resistance. the aim of the present study was to investigate whether priming of plant defense responses is a mechanism by which fo47 controls fusarium wilt. an in vitro design enabled inoculation of the tap root with fo47 and the pathogenic strain (fol8) at different locations and different times. the express ... | 2013 | 23617416 |
| quantitative and microscopic assessment of compatible and incompatible interactions between chickpea cultivars and fusarium oxysporum f. sp. ciceris races. | fusarium wilt caused by fusarium oxysporum f. sp. ciceris, a main threat to global chickpea production, is managed mainly by resistant cultivars whose efficiency is curtailed by fusarium oxysporum f. sp. ciceris races. | 2013 | 23613839 |
| characterization of an acid-tolerant β-1,4-glucosidase from fusarium oxysporum and its potential as an animal feed additive. | an extracellular β-glucosidase (bgl) from fusarium oxysporum was purified to homogeneity by a single chromatography step on a gel filtration column. the optimum activity of bgl on cellobiose was observed at ph 5.0 and 60 °c. under the same conditions, the k(m) and v(max) values for p-nitrophenyl β-d-glucopyranoside and cellobiose were 2.53 mm, 268 u mg protein(-1) and 20.3 mm, 193 u mg protein(-1), respectively. the f. oxysporum bgl enzyme was highly stable at acidic ph (t 1/2 = 470 min at ph 3) ... | 2013 | 23604557 |
| characterization and expression analysis of dirigent family genes related to stresses in brassica. | the dirigent (dir) genes are playing a vital role in enhancing stress resistance in different crop plants. in this study, we collected 29 dir like genes, two from a brassica rapa cv. osome full length cdna library and 27 from the b. rapa database designated as b. rapa dirigent (brdir) like genes. sequence analysis and a comparison study of these genes confirmed that seven were dirigent and the remaining 22 were dirigent like genes. expression analysis revealed an organ specific expression of the ... | 2013 | 23562798 |
| synthesis and antifungal activities of novel nicotinamide derivatives containing 1,3,4-oxadiazole. | pant diseases in agriculture are extremely difficult to control, which bring about for billions of dollars in economic losses worldwide each year; nicotinamide derivatives has attracted more and more attention in the field of pesticide due to their broad bioactivities. in an effort to discover new molecules with good antifungal activity, a series of nicotinamide derivatives containing 1,3,4-oxadiazole were synthesized and bio-assayed. | 2013 | 23561596 |
| functional analysis of the cars gene of fusarium fujikuroi. | the ascomycete fungus fusarium fujikuroi is a model system in the investigation of the biosynthesis of some secondary metabolites, such as gibberellins, bikaverin, and carotenoids. carotenoid-overproducing mutants, generically called cars, are easily obtained in this fungus by standard mutagenesis procedures. here we report the functional characterization of gene cars, responsible for this mutant phenotype. the identity of the gene was demonstrated through the finding of mutations in six indepen ... | 2013 | 23543145 |
| signaling specificity provided by the arabidopsis thaliana heterotrimeric g-protein γ subunits agg1 and agg2 is partially but not exclusively provided through transcriptional regulation. | the heterotrimeric g-protein complex in arabidopsis thaliana consists of one α, one ß and three γ subunits. while two of the γ subunits, agg1 and agg2 have been shown to provide functional selectivity to the gßγ dimer in arabidopsis, it is unclear if such selectivity is embedded in their molecular structures or conferred by the different expression patterns observed in both subunits. in order to study the molecular basis for such selectivity we tested genetic complementation of agg1- and agg2 dr ... | 2013 | 23520518 |