Publications

TitleAbstractYear
Filter
PMID
Filter
antifungal and phytotoxic activity of essential oil from root of senecio amplexicaulis kunth. (asteraceae) growing wild in high altitude-himalayan region.this work was aimed to evaluate the essential oil from root of medicinally important plant senecio amplexicaulis for chemical composition, antifungal and phytotoxic activity. the chemical composition analysed by gc/gc-ms showed the presence of monoterpene hydrocarbons in high percentage with marker compounds as α-phellandrene (48.57%), o-cymene (16.80%) and β-ocimene (7.61%). the essential oil exhibited significant antifungal activity against five phytopathogenic fungi, sclerotium rolfsii, macro ...201627498832
nitrate protects cucumber plants against fusarium oxysporum by regulating citrate exudation.fusarium wilt causes severe yield losses in cash crops. nitrogen plays a critical role in the management of plant disease; however, the regulating mechanism is poorly understood. using biochemical, physiological, bioinformatic and transcriptome approaches, we analyzed how nitrogen forms regulate the interactions between cucumber plants and fusarium oxysporum f. sp. cucumerinum (foc). nitrate significantly suppressed fusarium wilt compared with ammonium in both pot and hydroponic experiments. few ...201627481896
chitosan and oligochitosan enhance ginger (zingiber officinale roscoe) resistance to rhizome rot caused by fusarium oxysporum in storage.the ability of chitosan and oligochitosan to enhance ginger (zingiber officinale) resistance to rhizome rot caused by fusarium oxysporum in storage was investigated. both chitosan and oligochitosan at 1 and 5g/l significantly inhibited rhizome rot, with the best control at 5g/l. chitosan and oligochitosan applied at 5g/l also reduced weight loss, measured as a decrease in fresh weight, but did not affect soluble solids content or titratable acidity of rhizomes. the two compounds applied at 5g/l ...201627474591
biological preparation of chitosan nanoparticles and its in vitro antifungal efficacy against some phytopathogenic fungi.the aim of the present study was to prepare chitosan nanoparticles through biological method with high antifungal activities. chitosan nanoparticles were prepared by the addition of anionic proteins isolated from penicillium oxalicum culture to chitosan solutions. the formation of chitosan nanoparticles was preliminary confirmed by uv-vis spectrophotometric analysis. the physico-chemical properties of the chitosan nanoparticles were determined by size and zeta potential analysis, ftir analysis, ...201627474573
synthesis, structure and antimicrobial evaluation of a new gossypol triazole conjugates functionalized with aliphatic chains and benzyloxy groups.synthetic limitations in the copper-catalyzed azide alkyne cycloaddition (cuaac) on gossypol's skeleton functionalized with alkyne (2) or azide (3) groups have been indicated. modified approach to the synthesis of new gossypol-triazole conjugates yielded new compounds (24-31) being potential fungicides. spectroscopic studies of triazole conjugates 24-31 have revealed their structures in solution, i.e., the presence of enamine-enamine tautomeric forms and π-π stacking intramolecular interactions ...201627469129
fungal isolate optimized for biogenesis of silver nanoparticles with enhanced colloidal stability.understanding the synthesis and properties of nanomaterials is critical for reliable applications. biological systems, such as fungi, have been described as a "green" alternative to synthesis, yet knowledge gaps exist in terms of production variability, comparison with commercial products, and identifying a clear biological advantage over other synthesis methods. in this study, we evaluated 12 fungal isolates of fusarium oxysporum for ag nanoparticle production and characterized the resultant bi ...201627466012
first report of fusarium oxysporum species complex infection in zebrafish culturing system.fusarium oxysporum species complex (fosc) is a highly diverse fungus. recently, f. oxysporum infection was identified from zebrafish (danio rerio) culturing system in korea. initially, a rapid whitish smudge was appeared in the water with the fungal blooming on walls of fish tanks. microscopic studies were conducted on fungal hyphae, colony pigmentation and chlamydospore formation and the presence of macro- and microspores confirmed that the isolated fungus as f. oxysporum. furthermore, isolated ...201727451953
pseudo-outbreak of fusarium oxysporum associated with bronchoscopy. 201627424170
effector profiles distinguish formae speciales of fusarium oxysporum.formae speciales (ff.spp.) of the fungus fusarium oxysporum are often polyphyletic within the species complex, making it impossible to identify them on the basis of conserved genes. however, sequences that determine host-specific pathogenicity may be expected to be similar between strains within the same forma specialis. whole genome sequencing was performed on strains from five different ff.spp. (cucumerinum, niveum, melonis, radicis-cucumerinum and lycopersici). in each genome, genes for putat ...201627387256
association of effector six6 with vascular wilt symptoms caused by fusarium oxysporum on soybean.the fusarium oxysporum species complex (fosc) is a widely distributed group of fungi that includes both pathogenic and nonpathogenic isolates. in a previous study, isolates within the fosc collected primarily from soybean were assessed for the presence of 12 fungal effector genes. although none of the assayed genes was significantly associated with wilt symptoms on soybean, the secreted in xylem 6 (six6) gene was present only in three isolates, which all produced high levels of vascular wilt on ...201627349740
"coca got us here and now it's our weakness:" fusarium oxysporum and the political ecology of a drug war policy alternative in bolivia.a strain of fusarium oxysporum fungus is killing coca plants in the chapare coca growing region of bolivia. coca farmers are already constrained in the amount of coca they can grow under the government's community-based coca control approach, "social control." coca leaf is the main ingredient in cocaine, but it is also a traditional medicine and food, is economically vital to household incomes, and is a political symbol of the current government administration. bolivia's approach to coca control ...201627346463
native isolate of trichoderma: a biocontrol agent with unique stress tolerance properties.species of trichoderma are widely recognized for their biocontrol abilities, but seldom studied collectively, for their plant growth promotion, abiotic stress tolerance and bioremediation properties. our study is a concentrated effort to establish the potential of native isolate trichoderma harzianum ksnm (t103) to tolerate biotic (root pathogens) and abiotic stresses [high salt (100-1000 mm); heavy metal (chromium, nickel and zinc: 1-10 mm); pesticides: malathion (100-600 ppm), carbofuran (100- ...201627339311
antimicrobial and antitumor activity and diversity of endophytic fungi from traditional chinese medicinal plant cephalotaxus hainanensis li.endophytes from cephalotaxus hainanensis li, an important source of anti-leukemia drugs, have not been widely explored. in this study, 265 endophytic fungal isolates from c. hainanensis li were screened for antimicrobial activities against tilapia, banana, rice, and rape and for antitumor activities against human leukemia cell lines (k562, nb4, and hl-60). diversity was also analyzed. the results showed that 17.7% of the endophytic fungi had antimicrobial activities against at least three differ ...201627323030
the fonsix6 gene acts as an avirulence effector in the fusarium oxysporum f. sp. niveum - watermelon pathosystem.when infecting a host plant, the fungus fusarium oxysporum secretes several effector proteins into the xylem tissue to promote virulence. however, in a host plant with an innate immune system involving analogous resistance proteins, the fungus effector proteins may trigger resistance, rather than promoting virulence. identity of the effector genes of fusarium oxysporum f. sp. niveum (fon) races that affect watermelon (citrullus lanatus) are currently unknown. in this study, the six6 (secreted in ...201627320044
improvement of banana cv. rasthali (silk, aab) against fusarium oxysporum f.sp. cubense (vcg 0124/5) through induced mutagenesis: determination of ld50 specific to mutagen, explants, toxins and in vitro and in vivo screening for fusarium wilt resistance.shoot tips and in vitro grown proliferating buds of banana cv. rasthali (silk, aab) were treated with various concentrations and durations of chemical mutagens viz., ems, nan3 and des. ld50 for shoot tips based on 50% reduction in fresh weight was determined as 2% for 3 h, 0.02% for 5 h and 0.15% for 5 h, while for proliferating buds, they were 0.6% for 30 min, 0.01% for 2 h and 0.06% for 2 h for the mutagens ems, nan3 and des, respectively. subsequently, the mutated explants were screened in vi ...201627319054
three fusarium oxysporum mitogen-activated protein kinases (mapks) have distinct and complementary roles in stress adaptation and cross-kingdom pathogenicity.mitogen-activated protein kinase (mapk) cascades mediate cellular responses to environmental signals. previous studies in the fungal pathogen fusarium oxysporum have revealed a crucial role of fmk1, the mapk orthologous to saccharomyces cerevisiae fus3/kss1, in vegetative hyphal fusion and plant infection. here, we genetically dissected the individual and combined contributions of the three mapks fmk1, mpk1 and hog1 in the regulation of development, stress response and virulence of f. oxysporum ...201627301316
fungal phytopathogens encode functional homologues of plant rapid alkalinization factor (ralf) peptides.in this article, we describe the presence of genes encoding close homologues of an endogenous plant peptide, rapid alkalinization factor (ralf), within the genomes of 26 species of phytopathogenic fungi. members of the ralf family are key growth factors in plants, and the sequence of the ralf active region is well conserved between plant and fungal proteins. ralf1-like sequences were observed in most cases; however, ralf27-like sequences were present in the sphaerulina musiva and septoria populi ...201627291634
biogenic terbium oxide nanoparticles as the vanguard against osteosarcoma.the synthesis of inner transition metal nanoparticles via an ecofriendly route is quite difficult. this study, for the first time, reports synthesis of terbium oxide nanoparticles using fungus, fusarium oxysporum. the biocompatible terbium oxide nanoparticles (tb2o3 nps) were synthesized by incubating tb4o7 with the biomass of fungus f. oxysporum. multiple physical characterization techniques, such as uv-visible and photoluminescence spectroscopy, tem, saed, and zeta-potential were used to confi ...201627288964
effects of silicon treatment and inoculation with fusarium oxysporum f. sp. vasinfectum on cellular defences in root tissues of two cotton cultivars.silicon has been shown to enhance the resistance of plants to fungal and bacterial pathogens. here, the effect of potassium silicate was assessed on two cotton (gossypium hirsutum) cultivars subsequently inoculated with fusarium oxysporum f. sp. vasinfectum (fov). sicot 189 is moderately resistant whilst sicot f-1 is the second most resistant commercial cultivar presently available in australia.201627288509
selection and evaluation of micro-organisms for biocontrol of verticillium dahliae in olive.to identify potential biological control agents against verticillium wilt in olive through a mass screening approach.201627277382
vinegar residue compost as a growth substrate enhances cucumber resistance against the fusarium wilt pathogen fusarium oxysporum by regulating physiological and biochemical responses.fusarium wilt caused by the fungus fusarium oxysporum f. sp. cucumerinum (foc) is the most severe soil-borne disease attacking cucumber. to assess the positive effects of vinegar residue substrate (vrs) on the growth and incidence of fusarium wilt on cucumber, we determined the cucumber growth parameters, disease severity, defense-related enzyme and pathogenesis-related (pr) protein activities, and stress-related gene expression levels. in in vitro and pot experiments, we demonstrated the follow ...201627272925
dispensable chromosomes in fusarium oxysporum f. sp. lycopersici.the genomes of many filamentous fungi consist of a 'core' part containing conserved genes essential for normal development as well as conditionally dispensable (cd) or lineage-specific (ls) chromosomes. in the plant-pathogenic fungus fusarium oxysporum f. sp. lycopersici, one ls chromosome harbours effector genes that contribute to pathogenicity. we employed flow cytometry to select for events of spontaneous (partial) loss of either the two smallest ls chromosomes or two different core chromosom ...201627271322
influence of plant genotype on the cultivable fungi associated to tomato rhizosphere and roots in different soils.rhizosphere and root-associated microbiota are crucial in determining plant health and in increasing productivity of agricultural crops. to date, research has mainly focused on the bacterial dimension of the microbiota. however, interest in the mycobiota is increasing, since fungi play a key role in soil ecosystems. we examined the effect of plant genotype, soil, and of fusarium oxysporum f. sp. lycopersici (fol) on the cultivable component of rhizosphere and root-associated mycobiota of tomato. ...201627268246
detection and differentiation of fusarium oxysporum f. sp. lycopersici race 1 using loop-mediated isothermal amplification with three primer sets.fusarium oxysporum f. sp. lycopersici (fol) causes tomato wilt. based on the difference in pathogenicity towards tomato cultivars, fol is classified into three races. in this study, a rapid method is developed for the detection and discrimination of fol race 1 using a loop-mediated isothermal amplification (lamp) assay with two primer sets targeting a region of the nucleotide sequence of the six4 gene specific for race 1 and a primer set targeting the six5 gene, conserved in all known fol isolat ...201627248357
potential of pseudomonas putida pci2 for the protection of tomato plants against fungal pathogens.tomato is one of the most economically attractive vegetable crops due to its high yields. diseases cause significant losses in tomato production worldwide. we carried out polymerase chain reaction studies to detect the presence of genes encoding antifungal compounds in the dna of pseudomonas putida strain pci2. we also used liquid chromatography-electrospray tandem mass spectrometry to detect and quantify the production of compounds that increase the resistance of plants to diseases from culture ...201627246499
production and characterization of desmalonichrome relative binding affinity for uranyl ions in relation to other siderophores.siderophores are iron (fe)-binding secondary metabolites that have been investigated for their uranium-binding properties. previous work has focused on characterizing hydroxamate types of siderophores, such as desferrioxamine b, for their uranyl (uo2)-binding affinity. carboxylate forms of these metabolites hold potential to be more efficient chelators of uo2, yet they have not been widely studied. desmalonichrome is a carboxylate siderophore that is not commercially available and so was obtaine ...201627232848
dynamics of colonization and expression of pathogenicity related genes in fusarium oxysporum f.sp. ciceri during chickpea vascular wilt disease progression.fusarium wilt caused by fusarium oxysporum f.sp. ciceri (foc) is a constant threat to chickpea productivity in several parts of the world. understanding the molecular basis of chickpea-foc interaction is necessary to improve chickpea resistance to foc and thereby the productivity of chickpea. we transformed foc race 2 using green fluorescent protein (gfp) gene and used it to characterize pathogen progression and colonization in wilt-susceptible (jg62) and wilt-resistant (digvijay) chickpea culti ...201627227745
the components of rice and watermelon root exudates and their effects on pathogenic fungus and watermelon defense.watermelon (citrullus lanatus) is susceptible to wilt disease caused by the fungus fusarium oxysporum f. sp niveum (fon). intercropping management of watermelon/aerobic rice (oryza sativa) alleviates watermelon wilt disease, because some unidentified component(s) in rice root exudates suppress fon sporulation and spore germination. here, we show that the phenolic acid p-coumaric acid is present in rice root exudates only, and it inhibits fon spore germination and sporulation. we found that exoge ...201627217091
agroindustrial composts to reduce the use of peat and fungicides in the cultivation of muskmelon seedlings.environmental concerns about peat extraction in wetland ecosystems have increased. therefore, there is an international effort to evaluate alternative organic substrates for the partial substitution of peat. the aim of this work was to use different composts (c1-c10) obtained from the fruit and vegetable processing industry (pepper, carrot, broccoli, orange, artichoke residues, sewage sludge (citric and pepper) and vineyard pruning wastes) to produce added-value composts as growing media with su ...201727197924
isolation and identification of biocontrol agent streptomyces rimosus m527 against fusarium oxysporum f. sp. cucumerinum.actinomycetes have received considerable attention as biocontrol agents against fungal plant pathogens and as plant growth promoters. in this study, a total of 320 actinomycetes were isolated from various habitats in china. among which, 77 strains have been identified as antagonistic activities against fusarium oxysporum f. sp. cucumerinum which usually caused fusarium wilt of cucumber. of these, isolate actinomycete m527 not only displayed broad-spectrum antifungal activity but also showed the ...201627192632
a new biotype of fusarium oxysporum f. sp. lycopersici race 2 emerged by a transposon-driven mutation of avirulence gene avr1.emergence of races in fusarium oxysporum f. sp. lycopersici (fol) is caused by loss or mutation of at least one avirulence (avr) gene. the product of avr1 is a small protein (avr1) secreted by fol in tomato xylem sap during infection. this protein triggers fol race 1 specific resistance (i) in tomato, indicating that avr1 is an avr gene. deletion of avr1 in race 1 resulted in the emergence of race 2, and an additional mutation in avr2 generated race 3. previously, we reported a new biotype of ra ...201627190160
post-transcriptional silencing of the sge1 gene induced by a dsrna hairpin in fusarium oxysporum f. sp cubense, the causal agent of panama disease.fusarium oxysporum f. sp cubense (foc), the causal agent of panama disease, is responsible for economic losses in banana crops worldwide. the identification of genes that effectively act on pathogenicity and/or virulence may contribute to the development of different strategies for disease control and the production of resistant plants. the objective of the current study was to analyze the importance of sge1 gene expression in foc virulence through post-transcriptional silencing using a double-s ...201627173186
hyphal chemotropism in fungal pathogenicity.the ability to grow as filamentous hyphae defines the lifestyle of fungi. hyphae are exposed to a variety of chemical stimuli such as nutrients or signal molecules from mating partners and host organisms. how fungi sense and process this chemical information to steer hyphal growth is poorly understood. saccharomyces cerevisiae and neurospora crassa have served as genetic models for the identification of cellular components functioning in chemotropism. a recent study in the pathogen fusarium oxys ...201627150623
association of putative fungal effectors in fusarium oxysporum with wilt symptoms in soybean.fungi within the fusarium oxysporum species complex can cause root rot, seedling blight, and wilt of soybean. isolates recovered from soybean vary in aggressiveness and also the type of symptoms they produce. the aim of this study was to identify genetic markers to detect aggressive soybean wilt isolates. eighty isolates collected primarily from soybean were tested in the greenhouse for their ability to produce wilt symptoms using susceptible 'jack' soybean. the same 80 isolates were assessed fo ...201627146104
jasmonate signalling and defence responses in the model legume medicago truncatula-a focus on responses to fusarium wilt disease.jasmonate (ja)-mediated defences play important roles in host responses to pathogen attack, in particular to necrotrophic fungal pathogens that kill host cells in order to extract nutrients and live off the dead plant tissue. the root-infecting fungal pathogen fusarium oxysporum initiates a necrotrophic growth phase towards the later stages of its lifecycle and is responsible for devastating fusarium wilt disease on numerous legume crops worldwide. here we describe the use of the model legume me ...201627135231
h1-a, a compound isolated from fusarium oxysporum inhibits hepatitis c virus (hcv) ns3 serine protease.the present study was aimed to isolate the active compounds from the fermentation products of fusarium oxysporum, which had hepatitis c virus (hcv) ns3 protease inhibitory activity. a bioactive compound was isolated by reverse-phase silica-gel column chromatography, silica-gel column chromatography, semi-preparative reverse-phase high performance liquid chromatography (hplc), and then its molecular structure was elucidated based on the spectrosopic analysis. as a result, the compound (h1-a, 1) e ...201627114318
detection of plant growth enhancing features in psychrotolerant yeasts from patagonia (argentina).this study explores the biotechnological potential for plant production of twelve psychrotolerant yeasts strains from northwest-patagonia. these strains were isolated from different substrates associated with nothofagus sp. in native forests and vaccinium sp. in a commercial plantation. yeasts characterization was performed using in vitro assays to evaluate the production of auxin-like compounds and siderophores, ability to solubilize inorganic phosphate and to reduce common plant pathogen growt ...201627106520
regiospecific synthesis of prenylated flavonoids by a prenyltransferase cloned from fusarium oxysporum.due to their impressive pharmaceutical activities and safety, prenylated flavonoids have a high potent to be applied as medicines and nutraceuticals. biocatalysis is an effective technique to synthesize prenylated flavonoids. the major concern of this technique is that the microbe-derived prenyltransferases usually have poor regiospecificity and generate multiple prenylated products. in this work, a highly regiospecific prenyltransferase (fopt1) was found from fusarium oxysporum. it could recogn ...201627098599
[antibacterial components from artificially induced dragon's blood of dracaena cambodiana].ten compounds were isolated from the artificially induced dragon's blood of dracaena cambodiana by various column chromatographies on silica and sephadex lh-20 gel. based on spectral analysis of nmr and ms, their structures were identified as 3, 4-dihydroxyallylbenzene (1), 3', 4', 5'-trimethoxycinnamylalcohol (2), pinoresinol (3), (2r)-7, 4'-dihydroxy-8-methylflavane (4), (2r)-7,4'-dihydroxy-5-methoxy-8-methylflavane(5),(2s)-7,3'-dihydroxy-4'-methoxy-8-methylflavane(6) ,(2s)-4',7-dihydroxy-6, 8 ...201527062817
new fusaric acid derivatives from the endophytic fungus fusarium oxysporum and their phytotoxicity to barley leaves.chemical investigation of the endophytic fungus fusarium oxysporum isolated from fruits of drepanocarpus lunatus afforded eight new fusaric acid derivatives, fusaricates a-g, 1-7, and 10-hydroxy-11-chlorofusaric acid, 8, along with four known compounds. their structures were elucidated by one- and two-dimensional nmr as well as ms data and by comparison with the literature. the absolute configurations of fusaricates c-e, 3-5, were determined using chiral gc-ms. fusaricates a-g, 1-7, represent th ...201627050289
novel pyrazolo[3,4-b]pyridine derivatives: synthesis, characterization, antimicrobial and antiproliferative profile.three novel series of pyridine derivatives, namely schiff's bases, 4-thiazolidinones and azetidin-2-ones bearing pyrazolo[3,4-b]pyridine moiety, have been synthesized. the chemical structures of the synthesized compounds were characterized. the compounds were tested for their antimicrobial activity using the agar well diffusion and broth macrodilution methods. the compounds were also evaluated for their antiproliferative activity using the sulforhodamine b (srb) assay. the majority of the tested ...201627040621
effect of chinese medicinal herbal residues on microbial community succession and anti-pathogenic properties during co-composting with food waste.this study investigated the antimicrobial properties of chinese medicinal herbal residues (cmhrs) during its co-composting with food waste (fw) in two different ratios along with a control. inhibition on total microbial population were assessed while the numerically dominant microbes were isolated and their antagonistic effects were assessed. results indicate that the active ingredients persist in the composting mass did not affect the microbes unspecifically as revealed from almost similar bact ...201627039351
characterization and expression profiling of myb transcription factors against stresses and during male organ development in chinese cabbage (brassica rapa ssp. pekinensis).myb proteins comprise a large family of plant transcription factors that play regulatory roles in different biological processes such as plant development, metabolism, and defense responses. to gain insight into this gene superfamily and to elucidate its roles in stress resistance, we performed a comprehensive genome-wide identification, characterization, and expression analysis of myb genes in chinese cabbage (brassica rapa ssp. pekinensis). we identified 475 chinese cabbage myb genes, among wh ...201627038155
development and application of a rapid and visual loop-mediated isothermal amplification for the detection of sporisorium scitamineum in sugarcane.smut is a fungal disease with widespread prevalence in sugarcane planting areas. early detection and proper identification of sporisorium scitamineum are essential in smut management practices. in the present study, four specific primers targeting the core effector pep1 gene of s. scitamineum were designed. optimal concentrations of mg(2+), primer and bst dna polymerase, the three important components of the loop-mediated isothermal amplification (lamp) reaction system, were screened using a sin ...201627035751
nuclear dynamics and genetic rearrangement in heterokaryotic colonies of fusarium oxysporum.recent studies have shown horizontal transfer of chromosomes to be a potential key contributor to genome plasticity in asexual fungal pathogens. however, the mechanisms behind horizontal chromosome transfer in eukaryotes are not well understood. here we investigated the role of conidial anastomosis in heterokaryon formation between incompatible strains of fusarium oxysporum and determined the importance of heterokaryons for horizontal chromosome transfer. using live-cell imaging we demonstrate t ...201627013267
a six1 homolog in fusarium oxysporum f. sp. conglutinans is required for full virulence on cabbage.fusarium oxysporum is a soil-born fungus that induces wilt and root rot on a variety of plants. f. oxysporum f. sp. conglutinans (foc) can cause wilt disease on cabbage. this study showed that a homolog of six1 protein in the arabidopsis infecting isolate fo5176 (fo5176-six1) had four isoforms in the conidia of foc by proteomic analysis. thus, we analyzed the roles of protein foc-six1. gene expression analysis showed that, compared to the expression in mycelia, dramatically altered expression of ...201627010418
antifungal indole alkaloids from winchia calophylla.ten indole alkaloids (1-10) were obtained from an antifungal extract of winchia calophylla, of which two (2 and 4) were new. n(4)-methyl-10-hydroxyl-desacetylakuammilin (2) was an akuammiline-type indole alkaloid. n(1)-methyl-echitaminic acid (4) was an unusual zwitterion with a basic vincorine-type skeleton. this is the first report of 10 in w. calophylla. the structures of all of the compounds were determined based on spectroscopic data, and their bioactivities were assessed. compound 1 showed ...201627002397
production of fusaric acid by fusarium spp. in pure culture and in solid medium co-cultures.the ability of fungi isolated from nails of patients suffering from onychomycosis to induce de novo production of bioactive compounds in co-culture was examined. comparison between the metabolite profiles produced by sarocladium strictum, by fusarium oxysporum, and by these two species in co-culture revealed de novo induction of fusaric acid based on hrms. structure confirmation of this toxin, using sensitive microflow nmr, required only three 9-cm petri dishes of fungal culture. a targeted meta ...201626999098
dissection of trichoderma longibrachiatum-induced defense in onion (allium cepa l.) against fusarium oxysporum f. sp. cepa by target metabolite profiling.trichoderma spp. are versatile opportunistic plant symbionts that can cause substantial changes in the metabolism of host plants, thereby increasing plant growth and activating plant defense to various diseases. target metabolite profiling approach was selected to demonstrate that trichoderma longibrachiatum isolated from desert soil can confer beneficial agronomic traits to onion and induce defense mechanism against fusarium oxysporum f. sp. cepa (foc), through triggering a number of primary an ...201626993243
lipases and whole cell biotransformations of 2-hydroxy-2-(ethoxyphenylphosphinyl)acetic acid and its ester.a wide spectrum of commercially available lipases and microbial whole cells catalysts were tested for biotransformations of 2-hydroxy-2-(ethoxyphenylphosphinyl)acetic acid 1 and its butyryl ester. the best results were achieved for biocatalytic hydrolysis of ester: 2-butyryloxy-2-(ethoxyphenylphosphinyl)acetic acid 2 performed by lipase from candida cylindracea, what gave optically active products with 85% enantiomeric excess, 50% conversion degree and enantioselectivity 32.9 for one pair of ena ...201626989983
association analysis for disease resistance to fusarium oxysporum in cape gooseberry (physalis peruviana l).vascular wilt caused by fusarium oxysporum is the most important disease in cape gooseberry (physalis peruviana l.) in colombia. the development of resistant cultivars is considered one of the most cost-effective means to reduce the impact of this disease. in order to do so, it is necessary to provide breeders with molecular markers and promising germplasm for introgression of different resistance loci as part of breeding schemes. here we described an association mapping study in cape gooseberry ...201626988219
development of a thematic collection of musa spp accessions using scar markers for preventive breeding against fusarium oxysporum f. sp cubense tropical race 4.bananas are one of the most consumed fruits worldwide, but are affected by many pests and diseases. one of the most devastating diseases is fusarium wilt, caused by fusarium oxysporum f. sp cubense (foc). recently, fusarium tropical race 4 (foc tr4) has been causing irreparable damage, especially in asia and africa where it has devastated entire plantations, including areas with cavendish, which is known to be resistant to foc race 1. although this race is not yet present in brazil, results obta ...201626985964
production and characterization of highly thermostable β-glucosidase during the biodegradation of methyl cellulose by fusarium oxysporum.production of β-glucosidase from fusarium oxysporum was investigated during degradation of some cellulosic substrates (avicel, α-cellulose, carboxymethyl cellulose (cmc), and methylcellulose). optimized production of β-glucosidase using the cellulosic substrate that supported highest yield of enzyme was examined over 192 h fermentation period and varied ph of 3.0-11.0. the β-glucosidase produced was characterized for its suitability for industrial application. methyl cellulose supported the high ...201626977320
regulation of mir394 in response to fusarium oxysporum f. sp. cepae (foc) infection in garlic (allium sativum l).micrornas (mirnas) are a class of post-transcriptional regulators that negatively regulate gene expression through target mrna cleavage or translational inhibition and play important roles in plant development and stress response. in the present study, six conserved mirnas from garlic (allium sativum l.) were analyzed to identify differentially expressed mirnas in response to fusarium oxysporum f. sp. cepae (foc) infection. stem-loop rt-pcr revealed that mir394 is significantly induced in garlic ...201626973694
evaluation of the cytotoxic behavior of fungal extracellular synthesized ag nanoparticles using confocal laser scanning microscope.silver nanoparticles have been synthesized by subjecting a reaction medium to a fusarium oxysporum biomass at 28 °c for 96 h. the biosynthesized ag nanoparticles were characterized on the basis of their anticipated peak at 405 nm using uv-vis-nir spectroscopy. structural confirmation was evident from the characteristic x-ray diffraction (xrd) pattern, high-resolution transmission electron microscopy (hrtem) and the particle size analyzer. the ag nanoparticles were of dimension 40 ± 5 nm and sphe ...201626950118
comparative genomics and prediction of conditionally dispensable sequences in legume-infecting fusarium oxysporum formae speciales facilitates identification of candidate effectors.soil-borne fungi of the fusarium oxysporum species complex cause devastating wilt disease on many crops including legumes that supply human dietary protein needs across many parts of the globe. we present and compare draft genome assemblies for three legume-infecting formae speciales (ff. spp.): f. oxysporum f. sp. ciceris (foc-38-1) and f. sp. pisi (fop-37622), significant pathogens of chickpea and pea respectively, the world's second and third most important grain legumes, and lastly f. sp. me ...201626945779
genotyping of fusarium isolates from onychomycoses in colombia: detection of two new species within the fusarium solani species complex and in vitro antifungal susceptibility testing.fusariosis have been increasing in colombia in recent years, but its epidemiology is poorly known. we have morphologically and molecularly characterized 89 isolates of fusarium obtained between 2010 and 2012 in the cities of bogotá and medellín. using a multi-locus sequence analysis of rdna internal transcribed spacer, a fragment of the translation elongation factor 1-alpha (tef-1α) and of the rna-dependent polymerase subunit ii (rpb2) genes, we identified the phylogenetic species and circulatin ...201626943726
exchange of core chromosomes and horizontal transfer of lineage-specific chromosomes in fusarium oxysporum.horizontal transfer of supernumerary or lineage-specific (ls) chromosomes has been described in a number of plant pathogenic filamentous fungi. so far it was not known whether transfer is restricted to chromosomes of certain size or properties, or whether 'core' chromosomes can also undergo horizontal transfer. we combined a directed and a non-biased approach to determine whether such restrictions exist. selection genes were integrated into the genome of a strain of fusarium oxysporum pathogenic ...201626941045
fatty acid esters produced by lasiodiplodia theobromae function as growth regulators in tobacco seedlings.the botryosphaeriaceae are a family of trunk disease fungi that cause dieback and death of various plant hosts. this work sought to characterize fatty acid derivatives in a highly virulent member of this family, lasiodiplodia theobromae. nuclear magnetic resonance and gas chromatography-mass spectrometry of an isolated compound revealed (z, z)-9,12-ethyl octadecadienoate, (trivial name ethyl linoleate), as one of the most abundant fatty acid esters produced by l. theobromae. a variety of natural ...201626926564
fusarium oxysporum f.sp. radicis-lycopersici induces distinct transcriptome reprogramming in resistant and susceptible isogenic tomato lines.fusarium oxysporum f.sp. radicis-lycopersici (forl) is one of the most destructive necrotrophic pathogens affecting tomato crops, causing considerable field and greenhouse yield losses. despite such major economic impact, little is known about the molecular mechanisms regulating fusarium oxysporum f.sp. radicis-lycopersici resistance in tomato.201626920134
antifungal, anti-oomycete and phytotoxic effects of volatile organic compounds from the endophytic fungus xylaria sp. strain pb3f3 isolated from haematoxylon brasiletto.to determine the antifungal, anti-oomycete and phytotoxic activity; and chemical composition of the volatile organic compounds (vocs) produced by endophytic fungus xylaria sp. pb3f3 isolated from haematoxylon brasiletto karst.201626920072
glucosinolate composition of colombian accessions of mashua (tropaeolum tuberosum ruíz & pavón), structural elucidation of the predominant glucosinolate and assessment of its antifungal activity.the content of individual and total glucosinolates in 65 mashua tuber accessions (tropaeolum tuberosum) from the germplasm bank at universidad nacional de colombia was determined by reverse phase high-performance liquid chromatography on enzymatically desulfated extracts. the predominant glucosinolate was identified and the possible structure of the glucosinolate present in lower proportion was postulated from evidence obtained by high-performance liquid chromatography/mass spectrometry, (1) h a ...201626919585
detection of human pathogenic fusarium species in hospital and communal sink biofilms by using a highly specific monoclonal antibody.the fungus fusarium is well known as a plant pathogen, but has recently emerged as an opportunistic pathogen of humans. habitats providing direct human exposure to infectious propagules are largely unknown, but there is growing evidence that plumbing systems are sources of human pathogenic strains in the fusarium solani species complex (fssc) and fusarium oxysporum species complex (fosc), the most common groups infecting humans. here, a newly developed fusarium-specific monoclonal antibody (mab ...201626914362
how phytohormones shape interactions between plants and the soil-borne fungus fusarium oxysporum.plants interact with a huge variety of soil microbes, ranging from pathogenic to mutualistic. the fusarium oxysporum (fo) species complex consists of ubiquitous soil inhabiting fungi that can infect and cause disease in over 120 different plant species including tomato, banana, cotton, and arabidopsis. however, in many cases fo colonization remains symptomless or even has beneficial effects on plant growth and/or stress tolerance. also in pathogenic interactions a lengthy asymptomatic phase usua ...201626909099
comparison of fungal community in black pepper-vanilla and vanilla monoculture systems associated with vanilla fusarium wilt disease.long-term vanilla monocropping often results in the occurrence of vanilla fusarium wilt disease, seriously affecting its production all over the world. in the present study, vanilla exhibited significantly less fusarium wilt disease in the soil of a long-term continuously cropped black pepper orchard. the entire fungal communities of bulk and rhizosphere soils between the black pepper-vanilla system (i.e., vanilla cropped in the soil of a continuously cropped black pepper orchard) and vanilla mo ...201626903995
development of a multiplex pcr method to detect fungal pathogens for quarantine on exported cacti.major diseases in grafted cacti have been reported and fusarium oxysporum, bipolaris cactivora, phytophthora spp. and collectotrichum spp. are known as causal pathogens. these pathogens can lead to plant death after infection. therefore, some european countries have quarantined imported cacti that are infected with specific fungal pathogens. consequently, we developed pcr detection methods to identify four quarantined fungal pathogens and reduce export rejection rates of korean grafted cacti. th ...201626889115
retracted article: extract from the brown seaweed ascophyllum nodosum as an elicitor of resistance to fusarium oxysporum f. sp. radicis-lycopersici in tomato. 201626886193
molecular phylogeny, pathogenicity and toxigenicity of fusarium oxysporum f. sp. lycopersici.the present study aimed at the molecular characterization of pathogenic and non pathogenic f. oxysporum f. sp. lycopersici strains isolated from tomato. the causal agent isolated from symptomatic plants and soil samples was identified based on morphological and molecular analyses. pathogenicity testing of 69 strains on five susceptible tomato varieties showed 45% of the strains were highly virulent and 30% were moderately virulent. molecular analysis based on the fingerprints obtained through is ...201626883288
analysis of root-knot nematode and fusarium wilt disease resistance in cotton (gossypium spp.) using chromosome substitution lines from two alien species.chromosome substitution (cs) lines in plants are a powerful genetic resource for analyzing the contribution of chromosome segments to phenotypic variance. in this study, a series of interspecific cotton (gossypium spp.) cs lines were used to identify a new germplasm resource, and to validate chromosomal regions and favorable alleles associated with nematode or fungal disease resistance traits. the cs lines were developed in the g. hirsutum l. tm-1 background with chromosome or chromosome segment ...201626882892
biosynthesis of fluorescent bi2s3 nanoparticles and their application as dual-function spect-ct probe for animal imaging.bismuth sulphide (bi2s3) is an excellent semiconductor and its nanoparticles have numerous significant applications including photovoltaic materials, photodiode arrays, bio-imaging, etc. nevertheless, these nanoparticles when fabricated by chemical and physical routes tend to easily aggregate in colloidal solutions, are eco-unfriendly, cumbrous and very broad in size distribution. the aim of the present manuscript was to ecologically fabricate water dispersible, safe and stable bi2s3 nanoparticl ...201626876521
changes in the proteome of xylem sap in brassica oleracea in response to fusarium oxysporum stress.fusarium oxysporum f.sp. conlutinans (foc) is a serious root-invading and xylem-colonizing fungus that causes yellowing in brassica oleracea. to comprehensively understand the interaction between f. oxysporum and b. oleracea, composition of the xylem sap proteome of the non-infected and foc-infected plants was investigated in both resistant and susceptible cultivars using liquid chromatography-tandem mass spectrometry (lc-ms/ms) after in-solution digestion of xylem sap proteins. whole genome seq ...201626870056
development of a high-resolution melting marker for selecting fusarium crown and root rot resistance in tomato.fusarium crown and root rot is a severe fungal disease of tomato caused by fusarium oxysporum f. sp. radicis-lycopersici (forl). in this study, the genomic location of the forl-resistance locus was determined using a set of molecular markers on chromosome 9 and an f2 population derived from forl-resistant inbred 'av107-4' (solanum lycopersicum) × susceptible 'l3708' (solanum pimpinellifolium). bioassay performed using korean forl strain kacc 40031 showed single dominant inheritance of forl resis ...201626853773
nondermatophytic onychomycosis by fusarium oxysporum in an immunocompetent host.fusarium onychomycosis is not uncommon in tropical countries but is worth reporting. we report a case of nondermatophytic onychomycosis by fusarium oxysporum in an immunocompetent woman from buldhana district of maharashtra (india). bilateral involvement of great toe nail, chronic duration and acquisition of infection due to peculiar practice of daily pasting floors with mud and dung, is interesting. the case was successfully treated with topical and oral terbinafine with a dose of 250 mg daily ...201626852190
transcriptome profiling of resistance to fusarium oxysporum f. sp. conglutinans in cabbage (brassica oleracea) roots.fusarium wilt caused by fusarium oxysporum f. sp. conglutinans (foc) is a destructive disease of brassica crops, which results in severe yield losses. there is little information available about the mechanism of disease resistance. to obtain an overview of the transcriptome profiles in roots of r4p1, a brassica oleracea variety that is highly resistant to fusarium wilt, we compared the transcriptomes of samples inoculated with foc and samples inoculated with distilled water. rna-seq analysis gen ...201626849436
effects of cultivation ages and modes on microbial diversity in the rhizosphere soil of panax ginseng.panax ginseng cannot be cultivated on the same land consecutively for an extended period, and the underlying mechanism regarding microorganisms is still being explored.201626843819
the ftf gene family regulates virulence and expression of six effectors in fusarium oxysporum.the ftf (fusarium transcription factor) gene family comprises a single copy gene, ftf2, which is present in all the filamentous ascomycetes analysed, and several copies of a close relative, ftf1, which is exclusive to fusarium oxysporum. an rna-mediated gene silencing system was developed to target mrna produced by all the ftf genes, and tested in two formae speciales: f. oxysporum f. sp. phaseoli (whose host is common bean) and f. oxysporum f. sp. lycopersici (whose host is tomato). quantificat ...201626817616
success evaluation of the biological control of fusarium wilts of cucumber, banana, and tomato since 2000 and future research strategies.the fusarium wilt caused by fusarium oxysporum strains is the most devastating disease of cucumber, banana, and tomato. the biological control of this disease has become an attractive alternative to the chemical fungicides and other conventional control methods. in this review, the research trends and biological control efficiencies (bce) of different microbial strains since 2000 are reviewed in detail, considering types of microbial genera, inoculum application methods, plant growth medium and ...201726810104
fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics.molecular changes elicited by plants in response to fungal attack and how this affects plant-pathogen interaction, including susceptibility or resistance, remain elusive. we studied the dynamics in root metabolism during compatible and incompatible interactions between chickpea and fusarium oxysporum f. sp. ciceri (foc), using quantitative label-free proteomics and nmr-based metabolomics. results demonstrated differential expression of proteins and metabolites upon foc inoculations in the resist ...201626801007
isolation of n2 -fixing rhizobacteria from lolium perenne and evaluating their plant growth promoting traits.twenty one dinitrogen (n2 )-fixing bacteria were isolated from the rhizosphere of lolium perenne grown for more than 10 years without n-fertilization. the nearly complete sequence of the 16s rrna gene of each strain and pairwise alignments among globally aligned sequences of the 16s rrna genes clustered them into nine different groups. out of the 21 strains, 11 were members of genus bacillus, 3 belonged to each one of genera paenibacillus and pseudoxanthomonas, and the remaining 2 strains to eac ...201626781208
identification of a novel small cysteine-rich protein in the fraction from the biocontrol fusarium oxysporum strain cs-20 that mitigates fusarium wilt symptoms and triggers defense responses in tomato.the biocontrol effect of the non-pathogenic fusarium oxysporum strain cs-20 against the tomato wilt pathogen f. oxysporum f. sp. lycopersici (fol) has been previously reported to be primarily plant-mediated. this study shows that cs-20 produces proteins, which elicit defense responses in tomato plants. three protein-containing fractions were isolated from cs-20 biomass using size exclusion chromatography. exposure of seedling roots to one of these fractions prior to inoculation with pathogenic f ...201526779237
[fungal disease survey and pathogen identification on codonopsis tangshen in chongqing].to confirm the current major diseases and corresponding pathogens of codonopsis tangshen in chongqing.201526762048
differential responses of vanilla accessions to root rot and colonization by fusarium oxysporum f. sp. radicis-vanillae.root and stem rot (rsr) disease caused by fusarium oxysporum f. sp. radicis-vanillae (forv) is the most damaging disease of vanilla (vanilla planifolia and v. × tahitensis, orchidaceae). breeding programs aimed at developing resistant vanilla varieties are hampered by the scarcity of sources of resistance to rsr and insufficient knowledge about the histopathology of forv. in this work we have (i) identified new genetic resources resistant to rsr including v. planifolia inbreds and vanilla relati ...201526734032
suicidal germination for parasitic weed control.parasitic weeds of the genera striga and orobanche spp. cause severe yield losses in agriculture, especially in developing countries and the mediterranean. seeds of these weeds germinate by a chemical signal exuded by the roots of host plants. the radicle thus produced attaches to the root of the host plant, which can then supply nutrients to the parasite. there is an urgent need to control these weeds to ensure better agricultural production. the naturally occurring chemical signals are strigol ...201626733056
oxysporizoline, an antibacterial polycyclic quinazoline alkaloid from the marine-mudflat-derived fungus fusarium oxysporum. 201626732255
the role of strigolactones during plant interactions with the pathogenic fungus fusarium oxysporum.strigolactones (sls) do not influence spore germination or hyphal growth of fusarium oxysporum. mutant studies revealed no role for sls but a role for ethylene signalling in defence against this pathogen in pea. strigolactones (sls) play important roles both inside the plant as a hormone and outside the plant as a rhizosphere signal in interactions with mycorrhizal fungi and parasitic weeds. what is less well understood is any potential role sls may play in interactions with disease causing micr ...201626725046
a hemocyanin-derived antimicrobial peptide from the penaeid shrimp adopts an alpha-helical structure that specifically permeabilizes fungal membranes.hemocyanins are respiratory proteins with multiple functions. in diverse crustaceans hemocyanins can release histidine-rich antimicrobial peptides in response to microbial challenge. in penaeid shrimp, strictly antifungal peptides are released from the c-terminus of hemocyanins.201626708991
[molecular identification and in vitro susceptibility of fusarium from fungal keratitis in central china].to detect the genotypes and in vitro antifungal susceptibility of fusarium isolated from patients with fungal keratitis in central china.201526693650
marker-assisted selection of fusarium wilt-resistant and gynoecious melon (cucumis melo l.).in this study, molecular markers were designed based on the sex determination genes acs7 (a) and wip1 (g) and the domain in the fusarium oxysporum-resistant gene fom-2 (f) in order to achieve selection of f. oxysporum-resistant gynoecious melon plants. markers of a and f are cleaved amplified polymorphic sequences that distinguish alleles according to restriction analysis. twenty f1 and 1863 f2 plants derived from the crosses between the gynoecious line wi998 and the fusarium wilt-resistant line ...201526662419
evolution of nine microsatellite loci in the fungus fusarium oxysporum.the evolution of nine microsatellites and one minisatellite was investigated in the fungus fusarium oxysporum and sister taxa fusarium redolens and fusarium verticillioides. compared to other organisms, fungi have been reported to contain fewer and less polymorphic microsatellites. mutational patterns over evolutionary time were studied for these ten loci by mapping changes in core repeat numbers onto a phylogeny based on the sequence of the conserved translation elongation factor 1-α gene. the ...201626661928
reducing environmental risk of excessively fertilized soils and improving cucumber growth by caragana microphylla-straw compost application in long-term continuous cropping systems.continuous cropping is a common agricultural practice in the word. in china, farmers often apply excessive fertilizers to fields in an attempt to maintain yields in continuous cropping systems. however, this practice often results in high nutrient concentrations in soils, nutrient pollution in leaching water and more crop disease. here, we investigated 8 different soils from continuously cropped cucumbers in northern china that grouped into those with extremely high nutrient levels (ehnl) and th ...201626657371
liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of fusarium oxysporum.fusarium oxysporum l11 is a non-pathogenic soil-borne fungal strain that yielded an extract that showed antifungal activity against phytopathogens. in this study, reversed-phase high-performance liquid chromatography (rp-hplc) coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry (api-qtof-ms) was applied for the comprehensive profiling of the metabolites from the extract. the employed sources were electrospray (esi), atmospheric pressure chemic ...201626655791
an interspecies signaling system mediated by fusaric acid has parallel effects on antifungal metabolite production by pseudomonas protegens strain pf-5 and antibiosis of fusarium spp.pseudomonas protegens strain pf-5 is a rhizosphere bacterium that suppresses soilborne plant diseases and produces at least seven different secondary metabolites with antifungal properties. we derived mutants of pf-5 with single and multiple mutations in biosynthesis genes for seven antifungal metabolites: 2,4-diacetylphoroglucinol (dapg), pyrrolnitrin, pyoluteorin, hydrogen cyanide, rhizoxin, orfamide a, and toxoflavin. these mutants were tested for inhibition of the pathogens fusarium verticil ...201526655755
illumina miseq investigations on the changes of microbial community in the fusarium oxysporum f.sp. cubense infected soil during and after reductive soil disinfestation.although reductive soil disinfestation (rsd) is increasingly used for the control of soil-borne diseases, its impact on the soil microbial community during and after rsd remains poorly understood. miseq pyrosequencing, real-time pcr and denaturing gradient gel electrophoresis were performed to investigate the changes of microbial community in the fusarium oxysporum f. sp. cubense (foc) infected soil during rsd and at the simulative banana cultivation after rsd. the results showed that rsd signif ...201526640050
fusarium oxysporum volatiles enhance plant growth via affecting auxin transport and signaling.volatile organic compounds (vocs) have well-documented roles in plant-plant communication and directing animal behavior. in this study, we examine the less understood roles of vocs in plant-fungal relationships. phylogenetically and ecologically diverse strains of fusarium oxysporum, a fungal species complex that often resides in the rhizosphere of assorted plants, produce volatile compounds that augment shoot and root growth of arabidopsis thaliana and tobacco. growth responses of a. thaliana h ...201526617587
antibody-mediated pathogen resistance in plants.the methods described in this chapter were developed in order to produce transgenic plants expressing pathogen-specific single-chain variable fragment (scfv) antibodies fused to antifungal peptides (afps), conferring resistance against fungal pathogens. we describe the selection from a phage display library of avian scfv antibodies that recognize cell surface proteins on fungi from the genus fusarium, and the construction of scfv-afp fusion protein constructs followed by their transient expressi ...201626614296
identification of pathogenicity-related genes in fusarium oxysporum f. sp. cepae.pathogenic isolates of fusarium oxysporum, distinguished as formae speciales (f. spp.) on the basis of their host specificity, cause crown rots, root rots and vascular wilts on many important crops worldwide. fusarium oxysporum f. sp. cepae (foc) is particularly problematic to onion growers worldwide and is increasing in prevalence in the uk. we characterized 31 f. oxysporum isolates collected from uk onions using pathogenicity tests, sequencing of housekeeping genes and identification of effect ...201626609905
[keratomycosis due to fusarium oxysporum treated with the combination povidone iodine eye drops and oral fluconazole].in developing countries where systemic antifungal are often unavailable, treatment of filamentous fungi infection as fusarium is sometimes very difficult to treat. we report the case of a keratomycosis due to fusarium oxysporum treated by povidone iodine eye drops and oral fluconazole. the diagnosis of abscess in the cornea was retained after ophthalmological examination for a 28-year-old man with no previous ophthalmological disease, addressed to the ophthalmological clinic at the university ho ...201526597147
detailed mapping of a resistance locus against fusarium wilt in cultivated eggplant (solanum melongena).this is the first report on genetic mapping of a resistance locus against fusarium wilt caused by the plant pathogen fusarium oxysporum f. sp. melongenae in cultivated eggplant.201626582508
synthesis of novel β-keto-enol derivatives tethered pyrazole, pyridine and furan as new potential antifungal and anti-breast cancer agents.recently, a new generation of highly promising inhibitors bearing β-keto-enol functionality has emerged. reported herein is the first synthesis and use of novel designed drugs based on the β-keto-enol group embedded with heterocyclic moieties such as pyrazole, pyridine, and furan, prepared in a one-step procedure by mixed claisen condensation. all the newly synthesized compounds were characterized by ft-ir, ¹h-nmr, (13)c-nmr, esi/lc-ms, elemental analysis, and evaluated for their in vitro antipro ...201526569202
[genetic diversity analysis of fusarium oxysporum f. sp. cubense populations from china using inter-simple sequence repeats-pcr (issr-pcr) technique].we used inter-simple sequence repeats (issr) markers to reveal the genetic diversity of 95 fusarium oxysporum f. sp. cubense ( foc ) isolates from banana in china, for the rational control of the disease.201526562993
Displaying items 2301 - 2400 of 2935