Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| [inhibition of amaranthus retroflexus by wasp gut fungal isolate fusarium oxysporum mf06]. | to study the inhibitory effect of wasp gut fungus against the radicle growth of amaranthus retroflexus for the development of herbicides from microorganisms. | 2015 | 26065271 |
| [cloning and functional characterization of pathogenesis-related pr10-1 gene in panax notoginseng]. | with homology cloning approaches coupling with race (rapid-amplification of cdna ends) techniques, the full-length coding sequence of pathogenesis-related protein pr10-1 with differential expression was cloned from the total rna of the root of panax notoginseng, and its function was explored furtherly. as a result, the longest 465 bp orf (named as pnpr10-1 with the accession no. kj741402 in genbank) was detected from the cloned sequence with full-length of cdna of 863 bp. the corresponding pepti ... | 2015 | 25975034 |
| effects of water regime, crop residues, and application rates on control of fusarium oxysporum f. sp. cubense. | biological soil disinfestation is an effective method to control soil-borne disease by flooding and incorporating with organic amendments, but field conditions and resources sometimes limited its practical application. a laboratory experiment was conducted to develop practice guidelines on controlling fusarium wilt, a widespread banana disease caused by fusarium oxysporum f. sp. cubense (foc). foc infested soil incorporated with rice or maize straw at rates of 1.5 tons/ha and 3.0 tons/ha was inc ... | 2015 | 25968255 |
| the avr2-six5 gene pair is required to activate i-2-mediated immunity in tomato. | plant-invading microbes betray their presence to a plant by exposure of antigenic molecules such as small, secreted proteins called 'effectors'. in fusarium oxysporum f. sp. lycopersici (fol) we identified a pair of effector gene candidates, avr2-six5, whose expression is controlled by a shared promoter. the pathogenicity of avr2 and six5 fol knockouts was assessed on susceptible and resistant tomato (solanum lycopersicum) plants carrying i-2. the i-2 nb-lrr protein confers resistance to fol rac ... | 2015 | 25967461 |
| isolation of a hemagglutinin with potent antiproliferative activity and a large antifungal defensin from phaseolus vulgaris cv. hokkaido large pinto beans. | lectins (hemagglutinins) are defined as sugar-binding proteins or glycoproteins with various biological activities. a 60 kda dimeric hemagglutinin with a blocked n-terminus was isolated in large yield (190 mg/60 g) from the common edible bean phaseolus vulgaris cv. hokkaido large pinto bean. its hemagglutinating, antifungal, and antitumor activities as well as the effects of carbohydrate and metal ions on its hemagglutinating activity were examined. it inhibited the proliferation of nasopharynge ... | 2015 | 25965006 |
| demethylation of arsenic limits its volatilization in fungi. | arsenic (as) biomethylation is increasingly being regarded as a promising method to volatize as from the environment; however, the as volatilization efficiency of most microorganisms is low. here, the speciation transformation of dimethylarsinic acid (dma) as an important methylation intermediate in the cells of fusarium oxysporum cz-8f1, penicillium janthinellum sm-12f4, and trichoderma asperellum sm-12f1 were investigated. these fungal strains have been certified to volatilize as from as-loade ... | 2015 | 25951513 |
| effect of eugenol against streptococcus agalactiae and synergistic interaction with biologically produced silver nanoparticles. | streptococcus agalactiae (group b streptococci (gbs)) is an important infections agent in newborns associated with maternal vaginal colonization. intrapartum antibiotic prophylaxis in gbs-colonized pregnant women has led to a significant reduction in the incidence of early neonatal infection in various geographic regions. however, this strategy may lead to resistance selecting among gbs, indicating the need for new alternatives to prevent bacterial transmission and even to treat gbs infections. ... | 2015 | 25945115 |
| disseminated fusariosis and hematologic malignancies, a still devastating association. report of three new cases. | fungi of the genus fusarium are primarily plant pathogens and saprobes that produce disseminated infections in immunologically deficient humans. after aspergillosis, disseminated fusariosis is the second most common cause of invasive infection by filamentous fungi in patients with hematologic malignancies or those undergoing transplants of hematopoietic progenitors. | 2015 | 25936697 |
| metabolic profiling of chickpea-fusarium interaction identifies differential modulation of disease resistance pathways. | chickpea is the third most widely grown legume in the world and mainly used as a vegetarian source of human dietary protein. fusarium wilt, caused by fusarium oxysporum f. sp. ciceri (foc), is one of the major threats to global chickpea production. host resistance is the best way to protect crops from diseases; however, in spite of using various approaches, the mechanism of foc resistance in chickpea remains largely obscure. in the present study, non-targeted metabolic profiling at several time ... | 2015 | 25935544 |
| analyses of fusarium wilt race 3 resistance in upland cotton (gossypium hirsutum l.). | fusarium wilt [fusarium oxysporum f.sp. vasinfectum (fov) atk. sny & hans] represents a serious threat to cotton (gossypium spp.) production. for the last few decades, the fov pathogen has become a significant problem in uzbekistan causing severe wilt disease and yield losses of g. hirsutum l. cultivars. we present the first genetic analyses of fov race 3 resistance on uzbek cotton germplasm with a series of field and greenhouse artificial inoculation-evaluations and inheritance studies. the fie ... | 2015 | 25896369 |
| synthesis, insecticidal, and fungicidal screening of some new synthetic quinoline derivatives. | this paper describes the synthesis of a series of quinolines graphted with hydrazones, pyrazoles, pyridazine, phthalazine, triazepinone, semicarbazide, and thiomorpholide moieties and four metal complexes. these derivatives were screened against fusarium oxysporum and the red palm weevil (rpw) rhynchophorus ferrugineus oliver (coleopteran: curculionidae) as palm pathogens. only chlorinated quinolines were active against these organisms with hydrazones being good fungicides, while those modified ... | 2015 | 25895344 |
| n2o production, a widespread trait in fungi. | n2o is a powerful greenhouse gas contributing both to global warming and ozone depletion. while fungi have been identified as a putative source of n2o, little is known about their production of this greenhouse gas. here we investigated the n2o-producing ability of a collection of 207 fungal isolates. seventy strains producing n2o in pure culture were identified. they were mostly species from the order hypocreales order-particularly fusarium oxysporum and trichoderma spp.-and to a lesser extent s ... | 2015 | 25894103 |
| ethanol effect on metabolic activity of the ethalogenic fungus fusarium oxysporum. | fusarium oxysporum is a filamentous fungus which has attracted a lot of scientific interest not only due to its ability to produce a variety of lignocellulolytic enzymes, but also because it is able to ferment both hexoses and pentoses to ethanol. although this fungus has been studied a lot as a cell factory, regarding applications for the production of bioethanol and other high added value products, no systematic study has been performed concerning its ethanol tolerance levels. | 2015 | 25887038 |
| development of loop-mediated isothermal amplification (lamp) assay for rapid detection of fusarium oxysporum f. sp. ciceris - wilt pathogen of chickpea. | fusarium oxysporum f. sp. ciceris (foc), the causal agent of fusarium wilt is a devastating pathogen of chickpea. in chickpea, various soil borne pathogens produce (s) similar symptoms, therefore cannot be distinguished easily at field level. there is real need for a rapid, inexpensive, and easy to operate and maintain genotyping tool to facilitate accurate disease diagnosis and surveillance for better management of fusarium wilt outbreaks. | 2015 | 25886622 |
| gene expression patterns and dynamics of the colonization of common bean (phaseolus vulgaris l.) by highly virulent and weakly virulent strains of fusarium oxysporum. | the dynamics of root and hypocotyl colonization, and the gene expression patterns of several fungal virulence factors and plant defense factors have been analyzed and compared in the interaction of two fusarium oxysporum f. sp. phaseoli strains displaying clear differences in virulence, with a susceptible common bean cultivar. the growth of the two strains on the root surface and the colonization of the root was quantitatively similar although the highly virulent (hv) strain was more efficient r ... | 2015 | 25883592 |
| fungal cytochrome p450 monooxygenases of fusarium oxysporum for the synthesis of ω-hydroxy fatty acids in engineered saccharomyces cerevisiae. | omega hydroxy fatty acids (ω-ohfas) are multifunctional compounds that act as the basis for the production of various industrial products with broad commercial and pharmaceutical implications. however, the terminal oxygenation of saturated or unsaturated fatty acids for the synthesis of ω-ohfas is intricate to accomplish through chemocatalysis, due to the selectivity and controlled reactivity in c-h oxygenation reactions. cytochrome p450, the ubiquitous enzyme is capable of catalyzing the select ... | 2015 | 25880760 |
| priming of seeds with methyl jasmonate induced resistance to hemi-biotroph fusarium oxysporum f.sp. lycopersici in tomato via 12-oxo-phytodienoic acid, salicylic acid, and flavonol accumulation. | methyl jasmonate (meja) was tested by seed treatment for its ability to protect tomato seedlings against fusarium wilt caused by the soil-borne fungal pathogen fusarium oxysporum f.sp. lycopersici. isolated from solanum lycopersicon l. seeds, cv. beta fungus was identified as f. oxysporum f.sp. lycopersici race 3 fungus by using phytopathological and molecular methods. meja applied at 0.01, 0.1 and 1 mm reduced spore germination and mycelial growth in vitro. soaking of tomato seeds in meja solut ... | 2015 | 25867625 |
| glycogen catabolism, but not its biosynthesis, affects virulence of fusarium oxysporum on the plant host. | the role of glycogen metabolism was investigated in the fungal pathogen fusarium oxysporum. targeted inactivation was performed of genes responsible for glycogen biosynthesis: gnn1 encoding glycogenin, gls1 encoding glycogen synthase, and gbe1 encoding glycogen branching enzyme. moreover genes involved in glycogen catabolism were deleted: gph1 encoding glycogen phosphorylase and gdb1 encoding glycogen de-branching enzyme. glycogen reserves increased steadily during growth of the wild type strain ... | 2015 | 25865793 |
| mitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in fusarium oxysporum f. sp. cubense. | fusarium oxysporum f. sp. cubense (foc) is an important soil-borne fungal pathogen causing devastating vascular wilt disease of banana plants and has become a great concern threatening banana production worldwide. however, little information is known about the molecular mechanisms that govern the expression of virulence determinants of this important fungal pathogen. in this study, we showed that null mutation of three mitogen-activated protein (map) kinase genes, designated as foslt2, fomkk2 an ... | 2015 | 25849862 |
| [differential gene expression in incompatible interaction between lilium regale wilson and fusarium oxysporum f. sp. lilii revealed by combined ssh and microarray analysis]. | fusarium wilt, caused by a soilborne pathogen fusarium oxysporum f. sp. lilii, is the major disease of lily (lilium l.). in order to isolate the genes differentially expressed in a resistant reaction to f. oxysporum in l. regale wilson, a cdna library was constructed with l. regale root during f. oxysporum infection using the suppression subtractive hybridization (ssh), and a total of 585 unique expressed sequence tags (ests) were obtained. furthermore, the gene expression profiles in the incomp ... | 2015 | 25845232 |
| [screening probiotic endophytic bacteria from medicinal plant flex cornuta and the phytopathogen-inhibiting effect]. | culturable endophytic bacteria were isolated from medicinal plant ilex cornuta by plate-spreading method, strains with strong inhibitory effect on phytopathogen were screened by confrontation culture and fermentation filtrate culture methods, and the morphological changes of phytopathogen hyphae treated with endophytic bacteria were examined by microscopy and micrograph. their phylogenetic relationships were determined by homology analysis of the 16s rdna sequences of pcr products and the taxono ... | 2015 | 26571677 |
| combining uv-c treatment with biocontrol yeast to control postharvest decay of melon. | significant losses in harvested melon can be directly attributable to decay fungi. in the present study, the use of uv-c treatment combined with biocontrol yeast, pichia cecembensis, was evaluated for their ability to control postharvest decay of melon fruits after they were artificially inoculated with fusarium oxysporum and alternaria alternata. natural infection of fruit was also assessed. as a stand-alone treatment, uv-c or p. cecembensis significantly reduced fusarium rot and alternaria rot ... | 2015 | 25976334 |
| in vitro assessment of extracts of the lingzhi or reishi medicinal mushroom, ganoderma lucidum (higher basidiomycetes) against different plant pathogenic fungi. | five isolates of the lingzhi or reishi medicinal mushroom ganoderma lucidum (gl-1, gl-2, gl-3, gl-4, gl-5) were collected from different locations within and surrounding lahore, pakistan, to study the antifungal potential of their bioactive compounds. after studying morphology, different concentrations of the extracts were prepared in methanol and water using a soxhlet extractor. different cultures of fungal pathogens were acquired from the first fungal culture bank of pakistan, university of th ... | 2015 | 25954966 |
| [antifungal effect of phenolic and carotenoids extracts from chiltepin (capsicum annum var. glabriusculum) on alternaria alternata and fusarium oxysporum]. | the effect of phenolic and carotenoid extracts from chiltepin fruits on mycelial growth and the inhibition of conidial germination of alternaria alternata and fusarium oxysporum were investigated in the present work. phenolic extracts inhibited mycelial growth of a.alternata by 38.46%, and significantly reduced conidial germination on the fifth day after treatment to 92% in relation to control. no significant changes were observed in the inhibition of mycelial growth in fusarium oxysporum; howev ... | 2015 | 25705046 |
| fungal endophyte diversity and bioactivity in the indian medicinal plant ocimum sanctum linn. | endophytic mycopopulation isolated from india's queen of herbs tulsi (ocimum sanctum) were explored and investigated for their diversity and antiphytopathogenic activity against widespread plant pathogens botrytis cinerea, sclerotinia sclerotiorum, rhizoctonia solani and fusarium oxysporum. 90 fungal isolates, representing 17 genera were recovered from 313 disease-free and surface sterilised plant segments (leaf and stem tissues) from three different geographic locations (delhi, hyderabad and mu ... | 2015 | 26529087 |
| identification and characterization of an antifungal protein, afafpr9, produced by marine-derived aspergillus fumigatus r9. | a fungal strain, r9, was isolated from the south atlantic sediment sample and identified as aspergillus fumigatus. an antifungal protein, afafpr9, was purified from the culture supernatant of aspergillus fumigatus r9. afafpr9 was identified to be restrictocin, which is a member of the ribosome-inactivating proteins (rips), by maldi-tof-tof-ms. afafpr9 displayed antifungal activity against plant pathogenic fusarium oxysporum, alternaria longipes, colletotrichum gloeosporioides, paecilomyces vario ... | 2015 | 25394604 |
| diversity of food-borne bacillus volatile compounds and influence on fungal growth. | to evaluate the antifungal activity of the volatile organic compounds (vocs) produced by 75 different food-borne bacillus species against aspergillus niger, aspergillus flavus, aspergillus parasiticus, aspergillus clavatus, fusarium oxysporum f. sp. lactucae and moniliophthora perniciosa and to determine the vocs responsible for the inhibition. | 2015 | 25989039 |
| antifungal activity of violacein purified from a novel strain of chromobacterium sp. niist (mtcc 5522). | a novel strain of chromobacterium sp. niist (mtcc 5522) producing high level of purple blue bioactive compound violacein was isolated from clay mine acidic sediment. during 24 h aerobic incubation in modified luria bertani medium, around 0.6 g crude violacein was produced per gram of dry weight biomass. an inexpensive method for preparing crystalline, pure violacein from crude pigment was developed (12.8 mg violacein/l) and the pure compound was characterized by different spectrometric methods. ... | 2015 | 26428920 |
| characterization of the bioactive metabolites from a plant growth-promoting rhizobacteria and their exploitation as antimicrobial and plant growth-promoting agents. | a plant growth-promoting bacterial strain, pm 105, isolated from a tea plantation soil from the north eastern region of india was identified as pseudomonas aeruginosa through classical and 16s ribosomal dna (rdna) gene sequencing. further studies with this strain confirmed broad spectrum antifungal activity against ten human and plant pathogenic fungal pathogens viz. aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus tubingensis, candida albicans, colletotrichum gloeospori ... | 2015 | 25832181 |
| antimicrobial and inhibition on heat-induced protein denaturation of constituents isolated from polygonatum verticillatum rhizomes. | this study was designed to assess the susceptibility of various microorganisms and inhibition on heat-induced protein denaturation against diosgenin and santonin, isolated from polygonatum verticillatum rhizomes. both diosgenin and santonin showed significant zone of inhibition when studied against various gram-positive (bacillus subtilis, bacillus cereus, staphylococcus aureus and staphylococcus epidermidis) and gram-negative bacteria (escherichia coli and salmonella typhi). in antifungal assay ... | 2015 | 25553804 |
| in-vitro assessment of the probiotic potential of lactobacillus plantarum kcc-24 isolated from italian rye-grass (lolium multiflorum) forage. | the aim of the present study was to determine the probiotic potential of the lactic acid bacteria lactobacillus plantarum kcc-24 (l. plantarum kcc-24), that was isolated and characterized from italian ryegrass (lolium multiflorum) forage. the following experiments were performed to assess the probiotic characteristics such as antifungal activity, antibiotic susceptibility, resistance to low ph, stimulated gastric juice and bile salts, proteolytic activity, auto-aggregation, cell surface hydropho ... | 2015 | 25576238 |
| possible mechanism of antifungal phenazine-1-carboxamide from pseudomonas sp. against dimorphic fungi benjaminiella poitrasii and human pathogen candida albicans. | investigation of antifungal mechanism of phenazine 1-carboxamide (pc) produced by a pseudomonas strain mcc2142. | 2015 | 25348290 |
| probiotic potential of lactobacillus strains with antifungal activity isolated from animal manure. | the aim of the study was to isolate and characterize the lactic acid bacteria (lab) from animal manure. among the thirty lab strains, four strains, namely, kcc-25, kcc-26, kcc-27, and kcc-28, showed good cell growth and antifungal activity and were selected for further characterization. biochemical and physiology properties of strains confirmed that the strains are related to the lactobacillus sp.; further, the 16s rrna sequencing confirmed 99.99% sequence similarity towards lactobacillus planta ... | 2015 | 26167534 |
| minos as a novel tc1/mariner-type transposable element for functional genomic analysis in aspergillus nidulans. | transposons constitute powerful genetic tools for gene inactivation, exon or promoter trapping and genome analyses. the minos element from drosophila hydei, a tc1/mariner-like transposon, has proved as a very efficient tool for heterologous transposition in several metazoa. in filamentous fungi, only a handful of fungal-specific transposable elements have been exploited as genetic tools, with the impala tc1/mariner element from fusarium oxysporum being the most successful. here, we developed a t ... | 2015 | 26021704 |
| the effector repertoire of fusarium oxysporum determines the tomato xylem proteome composition following infection. | plant pathogens secrete small proteins, of which some are effectors that promote infection. during colonization of the tomato xylem vessels the fungus fusarium oxysporum f.sp. lycopersici (fol) secretes small proteins that are referred to as six (secreted in xylem) proteins. of these, six1 (avr3), six3 (avr2), six5, and six6 are required for full virulence, denoting them as effectors. to investigate their activities in the plant, the xylem sap proteome of plants inoculated with fol wild-type or ... | 2015 | 26583031 |
| investigating the association between flowering time and defense in the arabidopsis thaliana-fusarium oxysporum interaction. | plants respond to pathogens either by investing more resources into immunity which is costly to development, or by accelerating reproductive processes such as flowering time to ensure reproduction occurs before the plant succumbs to disease. in this study we explored the link between flowering time and pathogen defense using the interaction between arabidopsis thaliana and the root infecting fungal pathogen fusarium oxysporum. we report that f. oxysporum infection accelerates flowering time and ... | 2015 | 26034991 |
| the arabidopsis kh-domain rna-binding protein esr1 functions in components of jasmonate signalling, unlinking growth restraint and resistance to stress. | glutathione s-transferases (gsts) play important roles in the protection of cells against toxins and oxidative damage where one arabidopsis member, gstf8, has become a commonly used marker gene for early stress and defense responses. a gstf8 promoter fragment fused to the luciferase reporter gene was used in a forward genetic screen for arabidopsis mutants with up-regulated gstf8 promoter activity. this identified the esr1-1 (enhanced stress response 1) mutant which also conferred increased resi ... | 2015 | 25985302 |
| comparative genome analyses of serratia marcescens fs14 reveals its high antagonistic potential. | s. marcescens fs14 was isolated from an atractylodes macrocephala koidz plant that was infected by fusarium oxysporum and showed symptoms of root rot. with the completion of the genome sequence of fs14, the first comprehensive comparative-genomic analysis of the serratia genus was performed. pan-genome and cog analyses showed that the majority of the conserved core genes are involved in basic cellular functions, while genomic factors such as prophages contribute considerably to genome diversity. ... | 2015 | 25856195 |
| fusarium oxysporum triggers tissue-specific transcriptional reprogramming in arabidopsis thaliana. | some of the most devastating agricultural diseases are caused by root-infecting pathogens, yet the majority of studies on these interactions to date have focused on the host responses of aerial tissues rather than those belowground. fusarium oxysporum is a root-infecting pathogen that causes wilt disease on several plant species including arabidopsis thaliana. to investigate and compare transcriptional changes triggered by f. oxysporum in different arabidopsis tissues, we infected soil-grown pla ... | 2015 | 25849296 |
| isolation and heterologous expression of a polygalacturonase produced by fusarium oxysporum f. sp. cubense race 1 and 4. | fusarium wilt (panama disease) caused by fusarium oxysporum f. sp. cubense (foc) represents a significant threat to banana (musa spp.) production. musa aab is susceptible to race 1 (foc1) and race 4 (foc4), while cavendish musa aaa is found to be resistant to foc1 but still susceptible to race 4. a polygalacturonase (pgc3) was purified from the supernatant of fusarium oxysporum f. sp. cubense race 4 (foc4), which is the pathogen of fusarium wilt. pgc3 had an apparent molecular weight of 45 kda a ... | 2015 | 25854430 |
| skin and subcutaneous mycoses in tilapia (oreochromis niloticus) caused by fusarium oxysporum in coinfection with aeromonas hydrophila. | subcutaneous mycoses in freshwater fish are rare infections usually caused by oomycetes of the genus saprolegnia and some filamentous fungi. to date, fusarium infections in farmed fish have only been described in marine fish. here, we report the presence of fusarium oxysporum in subcutaneous lesions of nile tilapia (oreochromis niloticus). histopathologic evaluation revealed granuloma formation with fungal structures, and the identity of the etiological agent was demonstrated by morphological an ... | 2015 | 26155462 |
| ruta montana l. leaf essential oil and extracts: characterization of bioactive compounds and suppression of crown gall disease. | the aims of this study were to assess the antimicrobial efficacy of the leaf essential oil and the leaf extracts of r. montana against botrytis cinerea, fusarium oxysporum, verticillium dahliae, aspergillus oryzae and fusarium solani. the oil (1.000 µg/disk) and the extracts (1.500 µg/disk) revealed a remarkable antifungal effect against the tested plant pathogenic fungi with a radial growth inhibition percentage of 40.0-80.0 % and 5.0-58.0 %, respectively along with their respective mic values ... | 2015 | 26417353 |
| identification of an endophytic antifungal bacterial strain isolated from the rubber tree and its application in the biological control of banana fusarium wilt. | banana fusarium wilt (also known as panama disease) is one of the most disastrous plant diseases. effective control methods are still under exploring. the endophytic bacterial strain itbb b5-1 was isolated from the rubber tree, and identified as serratia marcescens by morphological, biochemical, and phylogenetic analyses. this strain exhibited a high potential for biological control against the banana fusarium disease. visual agar plate assay showed that itbb b5-1 restricted the mycelial growth ... | 2015 | 26133557 |
| transcriptome profiling of soybean (glycine max) roots challenged with pathogenic and non-pathogenic isolates of fusarium oxysporum. | fusarium oxysporum is one of the most common fungal pathogens causing soybean root rot and seedling blight in u.s.a. in a recent study, significant variation in aggressiveness was observed among isolates of f. oxysporum collected from roots in iowa, ranging from highly pathogenic to weakly or non-pathogenic isolates. | 2015 | 26689712 |
| differentially expressed genes in resistant and susceptible common bean (phaseolus vulgaris l.) genotypes in response to fusarium oxysporum f. sp. phaseoli. | fusarium wilt of common bean (phaseolus vulgaris l.), caused by fusarium oxysporum schlechtend.:fr. f.sp. phaseoli (fop), is one of the most important diseases of common beans worldwide. few natural sources of resistance to fop exist and provide only moderate or partial levels of protection. despite the economic importance of the disease across multiple crops, only a few of fop induced genes have been analyzed in legumes. therefore, our goal was to identify transcriptionally regulated genes duri ... | 2015 | 26030070 |
| diversity and activity of lysobacter species from disease suppressive soils. | the genus lysobacter includes several species that produce a range of extracellular enzymes and other metabolites with activity against bacteria, fungi, oomycetes, and nematodes. lysobacter species were found to be more abundant in soil suppressive against the fungal root pathogen rhizoctonia solani, but their actual role in disease suppression is still unclear. here, the antifungal and plant growth-promoting activities of 18 lysobacter strains, including 11 strains from rhizoctonia-suppressive ... | 2015 | 26635735 |
| diversity, distribution, and antagonistic activities of rhizobacteria of panax notoginseng. | rhizobacteria play an important role in plant defense and could be promising sources of biocontrol agents. this study aimed to screen antagonistic bacteria and develop a biocontrol system for root rot complex of panax notoginseng. | 2015 | 27158229 |
| screening and characterization of endophytic bacillus and paenibacillus strains from medicinal plant lonicera japonica for use as potential plant growth promoters. | a total of 48 endophytic bacteria were isolated from surface-sterilized tissues of the medicinal plant lonicera japonica, which is grown in eastern china; six strains were selected for further study based on their potential ability to promote plant growth in vitro (siderophore and indoleacetic acid production). the bacteria were characterized by phylogenetically analyzing their 16s rrna gene similarity, by examining their effect on the mycelial development of pathogenic fungi, by testing their p ... | 2015 | 26691455 |
| proteomic analysis reveals the positive roles of the plant-growth-promoting rhizobacterium nsy50 in the response of cucumber roots to fusarium oxysporum f. sp. cucumerinum inoculation. | plant-growth-promoting rhizobacteria (pgpr) can both improve plant growth and enhance plant resistance against a variety of environmental stresses. to investigate the mechanisms that pgpr use to protect plants under pathogenic attack, transmission electron microscopy analysis and a proteomic approach were designed to test the effects of the new potential pgpr strain paenibacillus polymyxa nsy50 on cucumber seedling roots after they were inoculated with the destructive phytopathogen fusarium oxys ... | 2016 | 28018395 |
| control of wilt and rot pathogens of tomato by antagonistic pink pigmented facultative methylotrophic delftia lacustris and bacillus spp. | the studies on the biocontrol potential of pink pigmented facultative methylotrophic (ppfm) bacteria other than the genus methylobacterium are scarce. in the present study, we report three facultative methylotrophic isolates; ppo-1, ppt-1, and ppb-1, respectively, identified as delftia lacustris, bacillus subtilis, and bacillus cereus by 16s rrna gene sequence analysis. hemolytic activity was tested to investigate the potential pathogenicity of isolates to plants and humans, the results indicate ... | 2016 | 27872630 |
| structural and functional characterization of a highly stable endo-β-1,4-xylanase from fusarium oxysporum and its development as an efficient immobilized biocatalyst. | replacing fossil fuel with renewable sources such as lignocellulosic biomass is currently a promising alternative for obtaining biofuel and for fighting against the consequences of climate change. however, the recalcitrant structure of lignocellulosic biomass residues constitutes a major limitation for its widespread use in industry. the efficient hydrolysis of lignocellulosic materials requires the complementary action of multiple enzymes including xylanases and β-xylosidases, which are respons ... | 2016 | 27602054 |
| novel enzyme family found in filamentous fungi catalyzing trans-4-hydroxylation of l-pipecolic acid. | hydroxypipecolic acids are bioactive compounds widely distributed in nature and are valuable building blocks for the organic synthesis of pharmaceuticals. we have found a novel hydroxylating enzyme with activity toward l-pipecolic acid (l-pip) in a filamentous fungus, fusarium oxysporum c8d. the enzyme l-pip trans-4-hydroxylase (pip4h) of f. oxysporum (fopip4h) belongs to the fe(ii)/α-ketoglutarate-dependent dioxygenase superfamily, catalyzes the regio- and stereoselective hydroxylation of l-pip ... | 2016 | 26801577 |
| detection of antibiotic-resistant bacteria endowed with antimicrobial activity from a freshwater lake and their phylogenetic affiliation. | antimicrobial resistance poses a serious challenge to global public health. in this study, fifty bacterial strains were isolated from the sediments of a freshwater lake and were screened for antibiotic resistance. out of fifty isolates, thirty-three isolates showed resistance against at least two of the selected antibiotics. analysis of 16s rdna sequencing revealed that the isolates belonged to ten different genera, namely staphylococcus(n = 8), bacillus(n = 7), lysinibacillus(n = 4), achromobac ... | 2016 | 27330861 |
| metabolic engineering of fusarium oxysporum to improve its ethanol-producing capability. | fusarium oxysporum is one of the few filamentous fungi capable of fermenting ethanol directly from plant cell wall biomass. it has the enzymatic toolbox necessary to break down biomass to its monosaccharides and, under anaerobic and microaerobic conditions, ferments them to ethanol. although these traits could enable its use in consolidated processes and thus bypass some of the bottlenecks encountered in ethanol production from lignocellulosic material when saccharomyces cerevisiae is used-namel ... | 2016 | 27199958 |
| fungal-mediated consolidated bioprocessing: the potential of fusarium oxysporum for the lignocellulosic ethanol industry. | microbial bioprocessing of lignocellulose to bioethanol still poses challenges in terms of substrate catabolism. the most important challenge is to overcome substrate recalcitrance and to thus reduce the number of steps needed to biorefine lignocellulose. conventionally, conversion involves chemical pretreatment of lignocellulose, followed by hydrolysis of biomass to monomer sugars that are subsequently fermented into bioethanol. consolidated bioprocessing (cbp) has been suggested as an efficien ... | 2016 | 26888202 |
| the concept of ecthyma gangrenosum illustrated by a fusarium oxysporum infection in an immunocompetent individual. | ecthyma gangrenosum (eg) involves necrotic cutaneous lesions caused by bacteria, mainly pseudomonas aeruginosa, and is usually seen in immunocompromised patients with septicemia. however, clinically similar infections have been published with fungi as etiologic agents. we present a case of an eg-like lesion due to fusarium oxysporum confirmed by clinical diagnosis, culture and molecular identification and discuss the definition of eg. | 2016 | 27324372 |
| identification, characterization and phylogenetic analysis of antifungal trichoderma from tomato rhizosphere. | the use of trichoderma isolates with efficient antagonistic activity represents a potentially effective and alternative disease management strategy to replace health hazardous chemical control. in this context, twenty isolates were obtained from tomato rhizosphere and evaluated by their antagonistic activity against four fungal pathogens (fusarium oxysporum f. sp. lycopersici, alternaria alternata, colletotrichum gloeosporoides and rhizoctonia solani). the production of extracellular cell wall d ... | 2016 | 27917337 |
| bioprospecting for secondary metabolites in the entomopathogenic bacterium photorhabdus luminescens subsp. sonorensis. | crude extracts of in vitro and in vivo cultures of two strains of photorhabdus l. sonorensis (enterobacteriaceae) were analyzed by tlc, hplc-uv and lc-ms. nine unique compounds with mass/charge ratios (m/z) ranging from 331.3 to 713.5 were found in ms analyses. bioactivity of extracts was assessed on a selection of plant pathogens/pests and non-target species. caborca strain extracts showed the highest activity against helicoverpa zea (lepidoptera: noctuidae) neonates at all concentrations teste ... | 2016 | 27702563 |
| molecular phylogeny, diversity, and bioprospecting of endophytic fungi associated with wild ethnomedicinal north american plant echinacea purpurea (asteraceae). | the endophytic fungal community associated with the ethnomedicinal plant echinacea purpurea was investigated as well as its potential for providing antifungal compounds against plant pathogenic fungi. a total of 233 endophytic fungal isolates were obtained and classified into 42 different taxa of 16 genera, of which alternaria alternata, colletotrichum dematium, and stagonosporopsis sp. 2 are the most frequent colonizers. the extracts of 29 endophytic fungi displayed activities against important ... | 2016 | 27273012 |
| synthesis and antifungal activity of 2h-1,4-benzoxazin-3(4h)-one derivatives. | a series of 2-alkyl-2h-1,4-benzoxazin-3(4h)-ones (4a-l) was easily synthesized by two-step process involving o-alkylation of 2-nitrophenols with methyl 2-bromoalkanoates and next "green" catalytic reductive cyclization of the obtained 2-nitro ester intermediates (3a-l). further, 6,7-dibromo (5a-c) and n-acetyl (6) derivatives were prepared by bromination and acetylation of unsubstituted 2-alkyl-2h-1,4-benzoxazin-3(4h)-ones (4a-c). the novel compounds (3a-l, 4d-l, 5a-c and 6) were fully character ... | 2016 | 26963527 |
| sesquiterpene emissions from alternaria alternata and fusarium oxysporum: effects of age, nutrient availability, and co-cultivation. | alternaria alternata is one of the most studied fungi to date because of its impact on human life - from plant pathogenicity to allergenicity. however, its sesquiterpene emissions have not been systematically explored. alternaria regularly co-occurs with fusarium fungi, which are common plant pathogens, on withering plants. we analyzed the diversity and determined the absolute quantities of volatile organic compounds (vocs) in the headspace above mycelial cultures of a. alternata and fusarium ox ... | 2016 | 26915756 |
| uptake of the fusarium effector avr2 by tomato is not a cell autonomous event. | pathogens secrete effector proteins to manipulate the host for their own proliferation. currently it is unclear whether the uptake of effector proteins from extracellular spaces is a host autonomous process. we study this process using the avr2 effector protein from fusarium oxysporum f. sp. lycopersici (fol). avr2 is an important virulence factor that is secreted into the xylem sap of tomato following infection. besides that, it is also an avirulence factor triggering immune responses in plants ... | 2016 | 28066471 |
| double-stranded rna-mediated interference of dumpy genes in bursaphelenchus xylophilus by feeding on filamentous fungal transformants. | rna interference (rnai) is a valuable tool for studying gene function in vivo and provides a functional genomics platform in a wide variety of organisms. the pinewood nematode, bursaphelenchus xylophilus, is a prominent invasive plant-parasitic nematode and has become a serious worldwide threat to forest ecosystems. presently, the complete genome sequence of b. xylophilus has been published, and research involving genome-wide functional analyses is likely to increase. in this study, we describe ... | 2016 | 26953254 |
| synergistic and additive effect of oregano essential oil and biological silver nanoparticles against multidrug-resistant bacterial strains. | bacterial resistance to conventional antibiotics has become a clinical and public health problem, making therapeutic decisions more challenging. plant compounds and nanodrugs have been proposed as potential antimicrobial alternatives. studies have shown that oregano (origanum vulgare) essential oil (oeo) and silver nanoparticles have potent antibacterial activity, also against multidrug-resistant strains; however, the strong organoleptic characteristics of oeo and the development of resistance t ... | 2016 | 27242772 |
| leveraging the attributes of mucor hiemalis-derived silver nanoparticles for a synergistic broad-spectrum antimicrobial platform. | driven by the need to engineer robust surface coatings for medical devices to prevent infection and sepsis, incorporation of nanoparticles has surfaced as a promising avenue to enhance non-fouling efficacy. microbial synthesis of such nanoscale metallic structures is of substantive interest as this can offer an eco-friendly, cost-effective, and sustainable route for further development. here we present a mucor hiemalis-derived fungal route for synthesis of silver nanoparticles, which display sig ... | 2016 | 28018316 |
| development of a taqman probe-based insulated isothermal polymerase chain reaction (iipcr) assay for detection of fusarium oxysporum f. sp. cubense race 4. | this study developed a novel and inexpensive detection method based on a taqman probe-based insulated isothermal polymerase chain reaction (iipcr) method for the rapid detection of panama disease caused by fusarium oxysporum f. sp. cubense (foc) race 4, which is currently among the most serious fungal vascular diseases worldwide. by using the portable pockit™ device with the novel primer set iifoc-1/iifoc-2, the foc race 4 iipcr assay (including dna amplification and signal monitoring) could be ... | 2016 | 27448242 |
| evaluation of the significance of cell wall polymers in flax infected with a pathogenic strain of fusarium oxysporum. | fusarium oxysporum infection leads to fusarium-derived wilt, which is responsible for the greatest losses in flax (linum usitatissimum) crop yield. plants infected by fusarium oxysporum show severe symptoms of dehydration due to the growth of the fungus in vascular tissues. as the disease develops, vascular browning and leaf yellowing can be observed. in the case of more virulent strains, plants die. the pathogen's attack starts with secretion of enzymes degrading the host cell wall. the main ai ... | 2016 | 27005923 |
| constitutive expression of a novel antimicrobial protein, hcm1, confers resistance to both verticillium and fusarium wilts in cotton. | fusarium and verticillium wilts, two of the most important diseases in cotton, pose serious threats to cotton production. here we introduced a novel antimicrobial protein hcm1, which comprised harpin protein from xanthomonas oryzae pv. oryzicola (xoc), and the chimeric protein, cecropin a-melittin, into cotton. the transgenic cotton lines with stable hcm1 expression showed a higher resistance to verticillium and fusarium wilts both in greenhouse and field trials compared to controls. hcm1 enable ... | 2016 | 26856318 |
| assessment of dapg-producing pseudomonas fluorescens for management of meloidogyne incognita and fusarium oxysporum on watermelon. | pseudomonas fluorescens isolates clinto 1r, wayne 1r, and wood 1r, which produce the antibiotic 2,4-diacetylphloroglucinol (dapg), can suppress soilborne diseases and promote plant growth. consequently, these beneficial bacterial isolates were tested on watermelon plants for suppression of meloidogyne incognita (root-knot nematode: rkn) and fusarium oxysporum f. sp. niveum (fon). in a greenhouse trial, wayne 1r root dip suppressed numbers of rkn eggs per gram root on 'charleston gray' watermelon ... | 2016 | 27168652 |
| rna-seq transcriptome response of flax (linum usitatissimum l.) to the pathogenic fungus fusarium oxysporum f. sp. lini. | fusarium oxysporum f. sp. lini is a hemibiotrophic fungus that causes wilt in flax. along with rust, fusarium wilt has become an important factor in flax production worldwide. resistant flax cultivars have been used to manage the disease, but the resistance varies, depending on the interactions between specific cultivars and isolates of the pathogen. this interaction has a strong molecular basis, but no genomic information is available on how the plant responds to attempted infection, to inform ... | 2016 | 27933082 |
| effects of consecutive monoculture of pseudostellaria heterophylla on soil fungal community as determined by pyrosequencing. | under consecutive monoculture, the biomass and quality of pseudostellaria heterophylla declines significantly. in this study, a three-year field experiment was conducted to identify typical growth inhibition effects caused by extended monoculturing of p. heterophylla. deep pyrosequencing was used to examine changes in the structure and composition of soil fungal community along a three-year gradient of monoculture. the results revealed a distinct separation between the newly planted plot and the ... | 2016 | 27216019 |
| characterization of a novel cotton subtilase gene gbsbt1 in response to extracellular stimulations and its role in verticillium resistance. | verticillium wilt is a disastrous vascular disease in plants caused by verticillium dahliae. verticillium pathogens secrete various disease-causing effectors in cotton. this study identified a subtilase gene gbsbt1 from gossypium babardense and investigated the roles against v. dahliae infection. gbsbt1 gene expression is responsive to v. dahliae defense signals, jasmonic acid, and ethylene treatments. moreover, the gbsbt1 protein is mainly localized in the cell membrane and moves into the cytop ... | 2016 | 27088499 |
| characterization of a jaz7 activation-tagged arabidopsis mutant with increased susceptibility to the fungal pathogen fusarium oxysporum. | in arabidopsis, jasmonate (ja)-signaling plays a key role in mediating fusarium oxysporum disease outcome. however, the roles of jasmonate zim-domain (jaz) proteins that repress ja-signaling have not been characterized in host resistance or susceptibility to this pathogen. here, we found most jaz genes are induced following f. oxysporum challenge, and screening t-dna insertion lines in arabidopsis jaz family members identified a highly disease-susceptible jaz7 mutant (jaz7-1d). this mutant exhib ... | 2016 | 26896849 |
| synthesis and evaluation of thiophene-based guanylhydrazones (iminoguanidines) efficient against panel of voriconazole-resistant fungal isolates. | a series of new thiophene-based guanylhydrazones (iminoguanidines) were synthesized in high yields using a straightforward two-step procedure. the antifungal activity of compounds was evaluated against a wide range of medicaly important fungal strains including yeasts, molds, and dermatophytes in comparison to clinically used drug voriconazole. cytotoxic properties of compounds were also determined using human lung fibroblast cell line and hemolysis assay. all guanylhydrazones showed significant ... | 2016 | 26867487 |
| plant defensins nad1 and nad2 induce different stress response pathways in fungi. | nicotiana alata defensins 1 and 2 (nad1 and nad2) are plant defensins from the ornamental tobacco that have antifungal activity against a variety of fungal pathogens. some plant defensins interact with fungal cell wall o-glycosylated proteins. therefore, we investigated if this was the case for nad1 and nad2, by assessing the sensitivity of the three aspergillus nidulans (an) o-mannosyltransferase (pmt) knockout (ko) mutants (an∆pmta, an∆pmtb, and an∆pmtc). an∆pmta was resistant to both defensin ... | 2016 | 27598152 |
| nitrogen metabolism and growth enhancement in tomato plants challenged with trichoderma harzianum expressing the aspergillus nidulans acetamidase amds gene. | trichoderma is a fungal genus that includes species that are currently being used as biological control agents and/or as biofertilizers. in addition to the direct application of trichoderma spp. as biocontrol agents in plant protection, recent studies have focused on the beneficial responses exerted on plants, stimulating the growth, activating the defenses, and/or improving nutrient uptake. the amds gene, encoding an acetamidase of aspergillus, has been used as a selectable marker for the trans ... | 2016 | 27536277 |
| carbon regulation of environmental ph by secreted small molecules that modulate pathogenicity in phytopathogenic fungi. | fruit pathogens can contribute to the acidification or alkalinization of the host environment. this capability has been used to divide fungal pathogens into acidifying and/or alkalinizing classes. here, we show that diverse classes of fungal pathogens-colletotrichum gloeosporioides, penicillium expansum, aspergillus nidulans and fusarium oxysporum-secrete small ph-affecting molecules. these molecules modify the environmental ph, which dictates acidic or alkaline colonizing strategies, and induce ... | 2016 | 26666972 |
| assessment of probiotic, antifungal and cholesterol lowering properties of pediococcus pentosaceus kcc-23 isolated from italian ryegrass. | lactic acid bacteria (lab) are important for the processing of various food products. although genetically modified organisms have contributed to improvements in various food products, there are some limitations. thus, the discovery of wild strains from natural sources must be considered as the most suitable approach for identifying new lab. therefore, we planned to isolate and characterise the lab from italian ryegrass forage and evaluate their biological potential. | 2016 | 25655225 |
| chemical profiling, antimicrobial and insecticidal evaluations of polygonum hydropiper l. | the emergence of multidrug resistant (mdr) pathogens is of great concern to the global health community. our ability to effectively treat diseases is based on the discovery of potent drugs for the treatment of these challenging diseases. traditional medicines are one of the major sources for the discovery of safe, effective and economical drug candidates. in order to validate its antibacterial, antifungal and insecticidal potentials with respect to traditional uses, we have screened for the firs ... | 2016 | 27919287 |
| phytogenic synthesis of silver nanoparticles, optimization and evaluation of in vitro antifungal activity against human and plant pathogens. | an attempt was made to synthesis of biocompatible silver nanoparticles from ten different cassia spp. among them, cassia roxburghii aqueous leaf extract supported the synthesis of highly efficient and stable agnps. the synthesis of agnps was optimized at different physico-chemical condition and highly stable agnps were synthesized with 1.0ml of c. roxburghii leaf extract, ph 7.0, 1.0mm agno3 and at 37°c. the synthesized agnps were characterized by xps, dls and zeta potential. dls and zeta potent ... | 2016 | 27664723 |
| discrimination of aspergillosis, mucormycosis, fusariosis, and scedosporiosis in formalin-fixed paraffin-embedded tissue specimens by use of multiple real-time quantitative pcr assays. | in a retrospective multicenter study, 102 formalin-fixed paraffin-embedded (ffpe) tissue specimens with histopathology results were tested. two 4- to 5-μm ffpe tissue sections from each specimen were digested with proteinase k, followed by automated nucleic acid extraction. multiple real-time quantitative pcr (qpcr) assays targeting the internal transcribed spacer 2 (its2) region of ribosomal dna, using fluorescently labeled primers, was performed to identify clinically important genera and spec ... | 2016 | 27605714 |
| in vitro experimental environments lacking or containing soil disparately affect competition experiments of aspergillus flavus and co-occurring fungi in maize grains. | in vitro experimental environments are used to study interactions between microorganisms, and to predict dynamics in natural ecosystems. this study highlights that experimental in vitro environments should be selected to match closely the natural environment of interest during in vitro studies to strengthen extrapolations about aflatoxin production by aspergillus and competing organisms. fungal competition and aflatoxin accumulation were studied in soil, cotton wool or tube (water-only) environm ... | 2016 | 27264786 |
| 2,3-dideoxyglucosides of selected terpene phenols and alcohols as potent antifungal compounds. | the antifungal activities of novel 2,3-unsaturated and 2,3-dideoxy 1-o-glucosides of carvacrol, thymol, and perillyl alcohol were tested against aspergillus flavus, aspergillus ochraceus, fusarium oxysporum, saccharomyces cerevisiae and candida albicans. in the agar well diffusion tests, zones of inhibition for the derivatives of carvacrol, thymol and perillyl alcohol were higher (15-30mm) in the case of filamentous fungi than those for the parent compounds. their mic and mfc values indicated th ... | 2016 | 27211660 |
| effect of polymer/nanosilver composite packaging on long-term microbiological status of iranian saffron (crocus sativus l.). | crocus sativus l. (saffron) is a valuable plant which is native to iran. saffron is the dried stigmata of the flowering part of the plant that is usually contaminated with different bacteria and fungi through production process. antimicrobial properties of silver nanoparticles are well recognized. to survey the effects of nanosilver packaging on microbiological status of spiked, saffron samples over a six month period were chosen. saffron samples from five regions of khorasan province were purch ... | 2016 | 27081358 |
| inhibition of filamentous fungi by ketoconazole-functionalized electrospun nanofibers. | nanotechnology strategies have been used for delivery and controlled release of antimicrobial drugs. electrospun nanofibers can be versatile vehicles to incorporate antimicrobials. in this work, poly-ε-caprolactone nanofibers functionalized with ketoconazole were produced by electrospinning and tested against filamentous fungi. ketoconazole-free nanofibers were produced as controls. functionalized nanofibers showed antifungal activity against aspergillus flavus, a. carbonarius, a. niger, aspergi ... | 2016 | 26775870 |
| an impedance-based mold sensor with on-chip optical reference. | a new miniaturized sensor system with an internal optical reference for the detection of mold growth is presented. the sensor chip comprises a reaction chamber provided with a culture medium that promotes the growth of mold species from mold spores. the mold detection is performed by measuring impedance changes with integrated electrodes fabricated inside the reaction chamber. the impedance change in the culture medium is caused by shifts in the ph (i.e., from 5.5 to 8) as the mold grows. in ord ... | 2016 | 27690039 |
| the f-box protein fbp1 functions in the invasive growth and cell wall integrity mitogen-activated protein kinase (mapk) pathways in fusarium oxysporum. | f-box proteins determine substrate specificity of the ubiquitin-proteasome system. previous work has demonstrated that the f-box protein fbp1, a component of the scf(fbp1) e3 ligase complex, is essential for invasive growth and virulence of the fungal plant pathogen fusarium oxysporum. here, we show that, in addition to invasive growth, fbp1 also contributes to vegetative hyphal fusion and fungal adhesion to tomato roots. all of these functions have been shown previously to require the mitogen-a ... | 2016 | 25808603 |
| combined action of the major secreted exo- and endopolygalacturonases is required for full virulence of fusarium oxysporum. | the genome of the tomato pathogen fusarium oxysporum f. sp. lycopersici encodes eight different polygalacturonases (pgs): four endopgs and four exopgs. quantitative real-time reverse transcription-polymerase chain reaction (rt-pcr) revealed that endopgs pg1 and pg5 and exopgs pgx4 and pgx6 are expressed at significant levels during growth on citrus pectin, polygalacturonic acid or the monomer galacturonic acid, as well as during the infection of tomato plants. the remaining pg genes exhibit low ... | 2016 | 26060046 |
| the neutral metallopeptidase nmp1 of trichoderma guizhouense is required for mycotrophy and self-defence. | trichoderma guizhouense njau 4742 (harzianum clade) can suppress the causative agent of banana wild disease fusarium oxysporum f. sp. cubense 4 (foc4). to identify genes involved in this trait, we used t-dna insertional mutagenesis and isolated one mutant that was unable to overgrow foc4 and had reduced antifungal ability. using the high-efficiency thermal asymmetric interlaced-pcr, the t-dna was located in the terminator of a neutral metalloprotease gene (encoding a merops family m35 protease), ... | 2016 | 26118314 |
| identification of i-7 expands the repertoire of genes for resistance to fusarium wilt in tomato to three resistance gene classes. | the tomato i-3 and i-7 genes confer resistance to fusarium oxysporum f. sp. lycopersici (fol) race 3 and were introgressed into the cultivated tomato, solanum lycopersicum, from the wild relative solanum pennellii. i-3 has been identified previously on chromosome 7 and encodes an s-receptor-like kinase, but little is known about i-7. molecular markers have been developed for the marker-assisted breeding of i-3, but none are available for i-7. we used an rna-seq and single nucleotide polymorphism ... | 2016 | 26177154 |
| comparative genomics of fusarium oxysporum f. sp. melonis reveals the secreted protein recognized by the fom-2 resistance gene in melon. | development of resistant crops is the most effective way to control plant diseases to safeguard food and feed production. disease resistance is commonly based on resistance genes, which generally mediate the recognition of small proteins secreted by invading pathogens. these proteins secreted by pathogens are called 'avirulence' proteins. their identification is important for being able to assess the usefulness and durability of resistance genes in agricultural settings. we have used genome sequ ... | 2016 | 26305378 |
| detailed mapping of a resistance locus against fusarium wilt in cultivated eggplant (solanum melongena). | this is the first report on genetic mapping of a resistance locus against fusarium wilt caused by the plant pathogen fusarium oxysporum f. sp. melongenae in cultivated eggplant. | 2016 | 26582508 |
| a conserved co-chaperone is required for virulence in fungal plant pathogens. | the maize pathogenic fungus ustilago maydis experiences endoplasmic reticulum (er) stress during plant colonization and relies on the unfolded protein response (upr) to cope with this stress. we identified the u. maydis co-chaperone, designated dnj1, as part of this conserved cellular response to er stress. ∆dnj1 cells are sensitive to the er stressor tunicamycin and display a severe virulence defect in maize infection assays. a dnj1 mutant allele unable to stimulate the atpase activity of chape ... | 2016 | 26487566 |
| antibody-mediated pathogen resistance in plants. | the methods described in this chapter were developed in order to produce transgenic plants expressing pathogen-specific single-chain variable fragment (scfv) antibodies fused to antifungal peptides (afps), conferring resistance against fungal pathogens. we describe the selection from a phage display library of avian scfv antibodies that recognize cell surface proteins on fungi from the genus fusarium, and the construction of scfv-afp fusion protein constructs followed by their transient expressi ... | 2016 | 26614296 |
| identification of pathogenicity-related genes in fusarium oxysporum f. sp. cepae. | pathogenic isolates of fusarium oxysporum, distinguished as formae speciales (f. spp.) on the basis of their host specificity, cause crown rots, root rots and vascular wilts on many important crops worldwide. fusarium oxysporum f. sp. cepae (foc) is particularly problematic to onion growers worldwide and is increasing in prevalence in the uk. we characterized 31 f. oxysporum isolates collected from uk onions using pathogenicity tests, sequencing of housekeeping genes and identification of effect ... | 2016 | 26609905 |
| evolution of nine microsatellite loci in the fungus fusarium oxysporum. | the evolution of nine microsatellites and one minisatellite was investigated in the fungus fusarium oxysporum and sister taxa fusarium redolens and fusarium verticillioides. compared to other organisms, fungi have been reported to contain fewer and less polymorphic microsatellites. mutational patterns over evolutionary time were studied for these ten loci by mapping changes in core repeat numbers onto a phylogeny based on the sequence of the conserved translation elongation factor 1-α gene. the ... | 2016 | 26661928 |
| reducing environmental risk of excessively fertilized soils and improving cucumber growth by caragana microphylla-straw compost application in long-term continuous cropping systems. | continuous cropping is a common agricultural practice in the word. in china, farmers often apply excessive fertilizers to fields in an attempt to maintain yields in continuous cropping systems. however, this practice often results in high nutrient concentrations in soils, nutrient pollution in leaching water and more crop disease. here, we investigated 8 different soils from continuously cropped cucumbers in northern china that grouped into those with extremely high nutrient levels (ehnl) and th ... | 2016 | 26657371 |
| liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of fusarium oxysporum. | fusarium oxysporum l11 is a non-pathogenic soil-borne fungal strain that yielded an extract that showed antifungal activity against phytopathogens. in this study, reversed-phase high-performance liquid chromatography (rp-hplc) coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry (api-qtof-ms) was applied for the comprehensive profiling of the metabolites from the extract. the employed sources were electrospray (esi), atmospheric pressure chemic ... | 2016 | 26655791 |
| suicidal germination for parasitic weed control. | parasitic weeds of the genera striga and orobanche spp. cause severe yield losses in agriculture, especially in developing countries and the mediterranean. seeds of these weeds germinate by a chemical signal exuded by the roots of host plants. the radicle thus produced attaches to the root of the host plant, which can then supply nutrients to the parasite. there is an urgent need to control these weeds to ensure better agricultural production. the naturally occurring chemical signals are strigol ... | 2016 | 26733056 |
| oxysporizoline, an antibacterial polycyclic quinazoline alkaloid from the marine-mudflat-derived fungus fusarium oxysporum. | 2016 | 26732255 |