Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| wounding induces resistance to pathogens with different lifestyles in tomato: role of ethylene in cross-protection. | many reports point to the existence of a network of regulatory signalling occurring in plants during the interaction with micro-organisms (biotic stress) and abiotic stresses such as wounding. however, the focus is on shared intermediates/components and/or common molecular outputs in differently triggered signalling pathways, and not on the degree and modes of effective influence between abiotic and biotic stresses nor the range of true plant-pathogen interactions open to such influence. we repo ... | 2007 | 17897407 |
| antimicrobial activity and the major components of the essential oil of plectranthus cylindraceus. | the traditional uses of the aerial parts of fresh plectranthus cylindraceus by the dhofaris in oman were investigated on the basis of antimicrobial properties and composition of its herb oil. | 2007 | 17897226 |
| heterologous expression of fusarium oxysporum tomatinase in saccharomyces cerevisiae increases its resistance to saponins and improves ethanol production during the fermentation of agave tequilana weber var. azul and agave salmiana must. | this paper describes the effect of the heterologous expression of tomatinase from fusarium oxysporum f. sp lycopersici in saccharomyces cerevisiae. the gene fotom1 under the control of the s. cerevisiae phosphoglycerate kinase (pgk1) promoter was cloned into pyes2. s. cerevisiae strain y45 was transformed with this vector and ura3 transformant strains were selected for resistance to alpha-tomatine. two transformants were randomly selected for further study (designated y45-1 and y45-2). control s ... | 2008 | 17896184 |
| a bowman-birk trypsin inhibitor with antiproliferative activity from hokkaido large black soybeans. | a trypsin inhibitor, with an n-terminal sequence highly homologous to those of 8-kda bowman-birk trypsin inhibitors, was isolated from the seeds of hokkaido large black soybeans. the trypsin inhibitor was unadsorbed on sp-sepharose but adsorbed on deae-cellulose and mono q. it inhibited proliferation in breast cancer (mcf-7) cells and hepatoma (hep g2) cells with an ic50 of 35 and 140 microm, respectively. the trypsin inhibitory activity of the inhibitor was completely preserved after exposure t ... | 2008 | 17880027 |
| antimicrobial activity of leaf extract of basilicum polystachyon (l) moench. | phenolic extract of leaves of basilicum polystachyon (l) moench was tested for in vitro antimicrobial activity against five bacteria (escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, bacillus subtilis, micrococcus leuteus) and three fungi (fusarium oxysporum, aspergillus niger, helminthosporium oryzae). efficacy of organic solvents, methanol and ethanol, as agents for extraction was compared with acidic water (2m; hcl). high-pressure liquid chromatographic (hplc) data showed that ... | 2007 | 17877153 |
| synthesis, antiviral and antifungal bioactivity of 2-cyano-acrylate derivatives containing phosphonyl moieties. | alkyl 2-cyano-3-methylthio-3-phosphonylacrylates were synthesized by the reaction of alkyl 2-cyano-3,3-dimethylthioacrylates with dialkyl phosphites. the structures of the new compounds were characterized by elemental analyses, ir, 1h-, 13c- and 31p-nmr spectral data. these compounds were tested in vitro against pathogenic fungi, namely, fusarium graminearum, cytospora mandshurica and fusarium oxysporum. amongst all compounds, 2d and 2t were found to be effective against the tested fungi at 50 m ... | 2007 | 17873832 |
| the chitin-binding cladosporium fulvum effector protein avr4 is a virulence factor. | the biotrophic fungal pathogen cladosporium fulvum (syn. passalora fulva) is the causal agent of tomato leaf mold. the avr4 protein belongs to a set of effectors that is secreted by c. fulvum during infection and is thought to play a role in pathogen virulence. previous studies have shown that avr4 binds to chitin present in fungal cell walls and that, through this binding, avr4 can protect these cell walls against hydrolysis by plant chitinases. in this study, we demonstrate that avr4 expressio ... | 2007 | 17849712 |
| a relatively stable antifungal peptide from buckwheat seeds with antiproliferative activity toward cancer cells. | an antifungal peptide with a molecular mass of approximately 4 kda was isolated from buckwheat seeds by using ion-exchange chromatography on sp-sepharose and q-sepharose, and gel filtration on superdex peptide. the peptide was adsorbed on sp-sepharose in 10 mm nh(4)oac buffer (ph 4.5) and on q-sepharose in 10 mm nh(4)hco(3) buffer (ph 9.4), and appeared to be highly purified after these two steps. it inhibited mycelial growth in fusarium oxysporum and mycosphaerella arachidicola with an ic(50) o ... | 2007 | 17828793 |
| the fibrinolytic activity of a novel protease derived from a tempeh producing fungus, fusarium sp. blb. | tempeh is a traditional indonesian soybean-fermented food produced by filamentous fungi, rhizopus sp. and fusarium sp. we isolated and sequenced the genomic gene and a cdna clone encoding a novel protease (fp) from fusarium sp. blb. the genomic gene was 856 bp in length and contained two introns. an isolated cdna clone encoded a protein of 250 amino acids. the predicted amino acid sequence of fp showed highest homology, of 76%, with that of trypsin from fusarium oxysporum. the hydrolysis activit ... | 2007 | 17827689 |
| [studies on purification and properties of antagonistic protein from bacteria ss02 of paenibacillus daejeonensis]. | the antifungal, anti-bacterical, anti-brine shrimp activities of sd22 isolated from paenibacillus daejeonensis bacteria ss02 were studied. the separation steps included ultracentrifugation, ultrafiltration and (nh4)2so4 fractional precipitation, further purification was performed by sephadexg-75 and deae-32 chromatography. its molecular weight determined by sds-page was 56.0 kd and its isoelectfic point was 6.4. sd22 was thermostable to some extent and stable to ultraviolet, but sensitive to som ... | 2007 | 17822044 |
| isolation and molecular analysis of r-gene in resistant zingiber officinale (ginger) varieties against fusarium oxysporum f.sp. zingiberi. | marker assisted selection (mas) of resistant varieties is a reliable and faster method of selecting the right varieties for cultivation. the aim of the present study is to find the genes responsible for resistance in highly resistant varieties. in the present work we report the presence of a resistance (r) gene of cc-nbs-lrr class of plant resistance genes. both direct pcr amplification from genomic dna as well as cdnas, yielded a 0.6 kb dna sequence indicating the absence of an intron. sequence ... | 2008 | 17804217 |
| longevity of fusarium oxysporum in soil tube culture. | in soil tube culture, representatives of three biologic forms of fusarium oxysporum survived unchanged morphologically for 11 years or more. an isolate of the muskmelon wilt fungus remained viable after 17 years' storage in dry air at a temperature of from 3 degrees to 4 degrees c. the surviving unit was found to be the chlamydospore. | 1961 | 17800128 |
| role of the transcriptional activator xlnr of fusarium oxysporum in regulation of xylanase genes and virulence. | fungal infection of plants involves degradation of the host cell wall through the action of lytic enzymes secreted by the pathogen. the role of these enzymes in virulence is difficult to determine due to their functional redundancy and, therefore, remains controversial. here, we have studied xlnr, a zinc-finger transcription factor from the vascular wilt pathogen fusarium oxysporum that is orthologous to the major transcriptional activator of xylanase genes in aspergillus spp. transcription of t ... | 2007 | 17722701 |
| characterization of protein changes associated with sugar beet (beta vulgaris) resistance and susceptibility to fusarium oxysporum. | fusarium oxysporum (f-19) is a serious threat to sugar beet. resistance exists, but the basis for resistance and disease is unknown. protein extracts from sugar beet genotypes c1200.xh024 (resistant, r) and fus7 (susceptible, s) were analyzed by multidimensional liquid chromatography at 2 and 5 days postinoculation (dpi) and compared to mock-inoculated controls. one hundred twenty-one (r) and 73 (s) protein peaks were induced/repressed by f-19, approximately 12 (r) and 8% (s) of the total proteo ... | 2007 | 17715886 |
| dna microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients. | the increasing incidence of invasive fungal infections (ifi) in immunocompromised patients emphasizes the need to improve diagnostic tools. we established a dna microarray to detect and identify dna from 14 fungal pathogens (aspergillus fumigatus, aspergillus flavus, aspergillus terreus, candida albicans, candida dubliniensis, candida glabrata, candida lusitaniae, candida tropicalis, fusarium oxysporum, fusarium solani, mucor racemosus, rhizopus microsporus, scedosporium prolificans, and trichos ... | 2007 | 17715373 |
| first isolation of an antifungal lipid transfer peptide from seeds of a brassica species. | an antifungal peptide with a molecular mass of 9412 and an n-terminal sequence exhibiting notable homology to those of lipid transfer proteins was isolated from seeds of the vegetable brassica campestris. the purification protocol entailed ion exchange chromatography on q-sepharose, affinity chromatography on affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatography (fplc) on mono s, and gel filtration by fplc on a superdex peptide column. the antifungal peptide was ad ... | 2007 | 17692430 |
| a robust identification and detection assay to discriminate the cucumber pathogens fusarium oxysporum f. sp. cucumerinum and f. sp. radicis-cucumerinum. | the fungal species fusarium oxysporum is a ubiquitous inhabitant of soils worldwide that includes pathogenic as well as non-pathogenic or even beneficial strains. pathogenic strains are characterized by a high degree of host specificity and strains that infect the same host range are organized in so-called formae speciales. strains for which no host plant has been identified are believed to be non-pathogenic strains. therefore, identification below the species level is highly desired. however, t ... | 2007 | 17686014 |
| purification and antimicrobial activity studies of the n-terminal fragment of ubiquitin from human amniotic fluid. | a 4.3-kda antimicrobial peptide was isolated from human amniotic fluid by dialysis, ultrafiltration, and c18 reversed-phase high performance liquid chromatography. this peptide, which we named amniotic fluid peptide-1 (afp-1), possessed antimicrobial activity but lacked hemolytic activity. in addition, afp-1 potently inhibited the growth of a variety of bacteria (escherichia coli, salmonella typhimurium, listeria monocytogenes and staphylococcus aureus), filamentous fungi (botrytis cinerea, aspe ... | 2007 | 17669700 |
| regulatory elements mediating expression of xylanase genes in fusarium oxysporum. | the role of dna regulatory elements mediating activation of the xylanase-encoding gene xyl4 by the transcription factor xlnr in the fungal pathogen fusarium oxysporum, was studied by in vitro and in vivo functional analysis of the xyl4 promoter. recombinant xlnr protein specifically bound the sequence ggctaa in electrophoretic mobility shift assays. experiments with xyl4 promoter fusions with the lacz reporter gene showed that the ggctaa sequence is required for xylan-induced transcriptional act ... | 2008 | 17664074 |
| characterization of the volatile constituents in the essential oil of pistacia lentiscus l. from different origins and its antifungal and antioxidant activity. | essential oil (eo) from aerial parts (leaves, juvenile branches, and flowers when present) of pistacia lentiscus l. growing wild in five localities of sardinia (italy) was extracted by steam-distillation (sd) and analyzed by gas chromatography (gc), fid, and gc-ion trap mass spectrometry (itms). samples of p. lentiscus l. were harvested between april and october to study the seasonal chemical variability of the eo. a total of 45 compounds accounting for 97.5-98.4% of the total eo were identified ... | 2007 | 17658828 |
| toxigenic fungi and mycotoxins in mature corn silage. | to investigate the exposure of livestock and farm workers to mycotoxins during the last months of silage use, the mycoflora and the mycotoxins in a mature silage (11-months-old) were studied. a multimycotoxin method was developed to evaluate the toxigenic in vitro ability of fungal strains. the screening of potentially toxigenic fungi isolated from the mature silage showed that six fusaria (fusarium culmorum, fusarium equiseti, fusarium graminearum, fusarium oxysporum, fusarium solani and fusari ... | 2007 | 17655998 |
| incidence and distribution of fusarium species associated with feeds and seeds from spain. | samples of seeds and feeds (corn-based and mixed) were collected during surveys in 1991-92 and 1992-93 from two regions of spain, one in northern spain where the annual rainfall is over 900 mm, and the other in southeastern spain where the annual rainfall is about 400 mm. the level of fusarium contamination was determined in the 657 samples analysed, and results were analysed statistically to assess the effects of type of sample and meteorological conditions on fusarium proliferation. the predom ... | 1998 | 17655403 |
| [presence of fusarium oxysporum in induced sputum from a patient with heatstroke. case report.]. | 1997 | 17655392 | |
| microbial toxicity of pesticide derivatives produced with uv-photodegradation. | our study aimed at acquiring information about the biological effect of pesticides and their degradates produced by uv-treatment on microbiological activity. five photosensitive pesticides (carbendazim, acetochlor, simazine, chlorpyrifos, eptc) and six representative soil microbes (bacillus subtilis, pseudomonas fluorescens, mycobacterium phlei, fusarium oxysporum, penicillium expansum, trichoderma harzianum) were applied throughout our model experiments. the antimicrobial effects of the pestici ... | 2007 | 17639315 |
| the effect of gossypium c-genome chromosomes on resistance to fusarium wilt in allotetraploid cotton. | fusarium oxysporum f. sp. vasinfectum (fov) has the potential to become the most economically significant pathogen of cotton in australia. although the levels of resistance present in the new commercial cultivars have improved significantly, they are still not immune and cotton breeders continue to look for additional sources of resistance. the native australian gossypium species represent an alternative source of resistance because they could have co-evolved with the indigenous fov pathogens. f ... | 2007 | 17632700 |
| a new steroidal saponin, yuccalan, from the leaves of yucca smalliana. | an extract of the leaves of yucca smalliana fern. (agavaceae) showed potential antimicrobial activity. employing a bioassay linked fractionation method, one of the active principles, namely yuccalan, was isolated as a new steroidal saponin. the structure of the new steroidal saponin was elucidated as 3-o-beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranosyl-(3beta, 5alpha, 6alpha, 25s)-spirostan-3,6,27-triol (1) using various spectroscopic techniques, including ir, ms, 1d and 2d 1h-nmr, and 13c-nm ... | 2007 | 17615670 |
| fungal multienzyme production on industrial by-products of the citrus-processing industry. | orange peels is the principal solid by-product of the citrus processing industry and the disposal of the fresh peels is becoming a major problem to many factories. dry citrus peels are rich in pectin, cellulose and hemicellulose and may be used as a fermentation substrate. production of multienzyme preparations containing pectinolytic, cellulolytic and xylanolytic enzymes by the mesophilic fungi aspergillus niger btl, fusarium oxysporum f3, neurospora crassa dsm 1129 and penicillium decumbens un ... | 2008 | 17604624 |
| influence of mineral amendment on disease suppressive activity of pseudomonas fluorescens to fusarium wilt of chickpea. | fusarium wilt caused by fusarium oxysporum f. sp. ciceri causes considerable yield loss of chickpea. pseudomonas fluorescens4-92 (pf4-92) strain can suppress the disease. amendment of zinc edta and copper edta could not suppress the disease significantly when used alone; however, they significantly suppressed the disease in presence of pf4-92. in vitro observation showed that at 40, 30 and 20microgml(-1) concentrations of these minerals, i.e. zn, cu and zn plus cu, respectively, completely repre ... | 2009 | 17604612 |
| rhizobacteria-induced priming in arabidopsis is dependent on ethylene, jasmonic acid, and npr1. | a nonpathogenic rhizobacterium, pseudomonas putida lsw17s, elicited systemic protection against fusarium wilt and pith necrosis caused by fusarium oxysporum f. sp. lycopersici and p. corrugata in tomato (lycopersicon esculentum l.). lsw17s also confers disease resistance against p. syringae pv. tomato dc3000 (dc3000) on arabidopsis ecotype col-0. to investigate mechanisms underlying disease protection, expression patterns of defense-related genes pr1, pr2, pr5, and pdf1.2 and cellular defense re ... | 2007 | 17601164 |
| alpha-tomatine, the major saponin in tomato, induces programmed cell death mediated by reactive oxygen species in the fungal pathogen fusarium oxysporum. | the tomato saponin alpha-tomatine has been proposed to kill sensitive cells by binding to cell membranes followed by leakage of cell components. however, details of the modes of action of the compound on fungal cells are poorly understood. in the present study, mechanisms involved in alpha-tomatine-induced cell death of fungi were examined using a filamentous pathogenic fungus fusarium oxysporum. alpha-tomatine-induced cell death of f. oxysporum (ticdf) occurred only under aerobic conditions and ... | 2007 | 17585910 |
| suppression of phytopathogenic fungi by hexane extract of nepenthes ventricosa x maxima leaf. | the hexane extract of nepenthes ventricosa x maxima leaf exhibited antifungal activity against alternaria alternata, aspergillus niger, bipolaris oryzae, fusarium oxysporum, phytophthora capsici, rhizoctonia solani, rhizopus stolonifer var. stolonifer and sclerotinia sclerotiorum. | 2007 | 17573203 |
| microbial consortium bioaugmentation of a polycyclic aromatic hydrocarbons contaminated soil. | in this study we evaluated the capacity of a defined microbial consortium (five bacteria: mycobacterium fortuitum, bacillus cereus, microbacterium sp., gordonia polyisoprenivorans, microbacteriaceae bacterium, naphthalene-utilizing bacterium; and a fungus identified as fusarium oxysporum) isolated from a pahs contaminated landfarm site to degrade and mineralize different concentrations (0, 250, 500 and 1000 mg kg(-1)) of anthracene, phenanthrene and pyrene in soil. pahs degradation and mineraliz ... | 2008 | 17572084 |
| synthesis and antifungal activity of some new 3-hydroxy-2-(1-phenyl-3-aryl-4-pyrazolyl) chromones. | seven new 3-hydroxy-2-(1-phenyl-3-aryl-4-pyrazolyl) chromones 4a-g have been synthesized by the oxidation of 2-hydroxychalcone analogues of pyrazole 3a-g with hydrogen peroxide (h(2)o(2)) in koh-meoh by algar flynn oymanda (afo) reaction. the structures of the compounds 4 were established by the combined use of (1)h nmr, ir and mass spectra. all the seven compounds were tested in vitro for their antifungal activity against three phytopathogenic fungi, namely helminthosporium species, fusarium ox ... | 2008 | 17555846 |
| [effects of root exudates from cucumber and squash on fusarium wilt occurrence]. | with the root exudates of two cucumber varieties jinyan 4 (susceptible variety) and jinchun 4 (resistant variety) and of black seed squash variety as test materials, this paper studied their effects and action mechanisms on the occurrence of fusarium wilt on jinyan 4. the results showed that the occurrence of fusarium wilt was earlier when treated with the root exudates of jinyan 4, and the infection rate was significantly higher at 15 days after inoculation, but nearly the same as the control a ... | 2007 | 17552193 |
| effects of root isoquinoline alkaloids from hydrastis canadensis on fusarium oxysporum isolated from hydrastis root tissue. | goldenseal (hydrastis canadensis l.) is a popular medicinal plant distributed widely in north america. the rhizome, rootlets, and root hairs produce medicinally active alkaloids. berberine, one of the hydrastis alkaloids, has shown antifungal activity. the influence of a combination of the major hydrastis alkaloids on the plant rhizosphere fungal ecology has not been investigated. a bioassay was developed to study the effect of goldenseal isoquinoline alkaloids on three fusarium isolates, includ ... | 2007 | 17549565 |
| treatment of chickpea with rhizobium isolates enhances the expression of phenylpropanoid defense-related genes in response to infection by fusarium oxysporum f. sp. ciceris. | differential expression of phenylalanine ammonia-lyase (pal), chalcone synthase (chs) and isoflavone reductase (ifr) genes involved in phenylpropanoids metabolism was investigated using northern blot analyses in chickpea seedlings bacterized with rhizobium isolates (pchdms and pch43) and further challenged with fusarium oxysporum f. sp. ciceris (foc) race 0. gene activation patterns in the moderately resistant accession inrat87/1 were compared with those exhibited by the susceptible accession il ... | 2007 | 17544286 |
| a novel tissue-specific plantain beta-1,3-glucanase gene that is regulated in response to infection by fusarium oxysporum fsp. cubense. | a new full-length beta-1,3-glucanase cdna, mpglu, was isolated from a plantain (musa paradisica) by the rapid amplification of cdna ends (race) technique. recombinant gst-mpglu protein, expressed in e. coli, hydrolyzed (1-->3),(1-->6)-beta-glucan of laminaria digitata and inhibited the growth of fusarium oxysporum fsp. cubense (race 4) suggesting that it is a beta-1,3-glucanase. southern blot analysis indicated that there is one copy of mpglu in the plantain genome. mpglu gene expression was det ... | 2007 | 17530180 |
| isoforms of trehalase and invertase of fusarium oxysporum. | enzymatic assays and native page were used to study trehalase and invertase activities, depending on culture age and different sugar conditions, in cell-free extracts, culture filtrates and ribosomal wash of fusarium oxysporum. the activity of invertase preceded that of trehalase; in the exponential phase of growth, mainly invertase activity was produced, whereas trehalase activity was high in the stationary phase. in this last phase of growth, the activity of intracellular trehalase was repress ... | 2007 | 17512710 |
| collimonas fungivorans, an unpredicted in vitro but efficient in vivo biocontrol agent for the suppression of tomato foot and root rot. | although bacteria from the genus collimonas have demonstrated in vitro antifungal activity against many different fungi, they appeared inactive against the plant-pathogenic fungus fusarium oxysporum f.sp. radicis-lycopersici (forl), the causal agent of tomato foot and root rot (tfrr). visualization studies using fluorescently labelled organisms showed that bacterial cells attached extensively to the fungal hyphae under nutrient-poor conditions but not in glucose-rich armstrong medium. collimonas ... | 2007 | 17504497 |
| the gene coding for a new transcription factor (ftf1) of fusarium oxysporum is only expressed during infection of common bean. | we report the isolation and analysis of the gene encoding ftf1 (fusarium transcription factor 1), a previously undescribed putative transcription factor from highly virulent strains of fusarium oxysporum f.sp. phaseoli that is transcribed specifically during early stages of infection of its host common bean (phaseolus vulgaris l.). the predicted 1080 amino acid ftf1 protein contains a zn(ii)2-cys6 binuclear cluster dna-binding motif. ftf1 expression during axenic growth in culture was not detect ... | 2007 | 17462924 |
| study on the diversity of endophytic communities from rice (oryza sativa l.) and their antagonistic activities in vitro. | endophytic populations were isolated from 2400 segments of oryza sativa collected from bhadra river project area, southern india during december 2005 (winter) and april 2006 (summer). overall colonization rates from surface sterilized tissues were 40.3% in roots and 25.83% in leaves during winter season, 20.15% in roots and 8.66% in leaves during summer season. nineteen different fungal taxa, a streptomyces sp. and bacterial species were isolated. streptomyces sp., chaetomium globosum, penicilli ... | 2009 | 17433644 |
| [study on the comparison of rdna and its sequence of fumonisin-producing strains]. | to study the phylogenetic relationships of fumonisin-producing fusarium verticillioides isolates from china and gibberella fujikuroi species complex. | 2007 | 17424850 |
| synthesis and antifungal activity of novel s-substituted 6-fluoro-4-alkyl(aryl)thioquinazoline derivatives. | 6-fluoro-4-quinazolinol is prepared by the cyclization reaction of 2-amino-5-fluorobenzoic acid and formamide. the resulting thiol obtained by treatment of hydroxyl group with phosphorus (v) sulfide is converted under phase transfer condition to 4-substituted 4-alkylthio-6-fluoroquinazoline derivatives by reaction with halide. the structures of the compounds are confirmed by elemental analysis, ir, and (1)h nmr. title compounds 3a, 3g, and 3h are found to possess good antifungal activities. usin ... | 2007 | 17412601 |
| co-inoculation of an antibiotic-producing bacterium and a lytic enzyme-producing bacterium for the biocontrol of tomato wilt caused by fusarium oxysporum f. sp. lycopersici. | the antifungal compound 2,4-diacetylphloroglucinol-producing bacterium, pseudomonas fluorescens strain lrb3w1, inhibits the growth of fusarium oxysporum f. sp. lycopersici, and controls fusarium wilt of tomato caused by f. oxysporum f. sp. lycopersici. on the other hand, serratia marcescens strain b2, which produces cell wall-degrading enzyme chitinases, did not inhibit fungal growth and the suppressive effect of strain b2 against tomato fusarium wilt was less than that of strain lrb3w1. combine ... | 2007 | 17408002 |
| [acanthamoeba keratitis: about the first two tunisian cases]. | acanthamoeba keratitis is a rare but severe corneal infection which, despite improvements in diagnosis and treatment, still culminates in prolonged morbidity and significant loss of visual acuity. we present the case report of the first identification of acanthamoeba as a causative agent of keratitis in tunisia. case no 1: a 20-year-old girl, nearsighted corrected with soft contact lenses, suffering from a deep corneal inflammation and poor visual acuity the ophthalmological examination showed b ... | 2007 | 17402694 |
| species-specific primers for fusarium redolens and a pcr-rflp technique to distinguish among three clades of fusarium oxysporum. | the currently available morphological and molecular diagnostic techniques for fusarium redolens and the three phylogenetic clades of fusarium oxysporum are problematic. aligned translation elongation factor 1 alpha (tef-1 alpha) gene sequences from these species and their close relatives were used to design f. redolens-specific primers, and to identify restriction sites that discriminate among the three clades of f. oxysporum. the f. redolens-specific primers distinguished this species from all ... | 2007 | 17391363 |
| the effects of fusarium oxysporum on broomrape (orobanche egyptiaca) seed germination. | broomrape (orobanche aegyptiaca l.), one of the most important parasitic weeds in iran, is a root parasitic plant that can attack several crops such as tobacco, sunflower, tomato and etc. several methods were used for orobanche control, however these methods are inefficient and very costly. biological control is an additional recent tool for the control of parasitic weeds. in order to study of the fungus fusarium oxysporum (biocontrol agent) effects on broomrape seed germination, two laboratory ... | 2006 | 17390893 |
| induced resistance in melon plants against fusarium oxysporum f.sp. melonis caused by a nonpathogenic isolate of the same formae specialis. | melon 'charentais t' susceptible to fusarium oxysporum f. sp. melonis (fom) race 1.2 was pre-inoculated, as a resistance inducer agent, with a nonpathogenic isolate of fom. the biocontrol agent was only effective when it was applied one or two days before a challenge inoculation with the pathogen. simultaneous inoculation did not provide any durable protection. melon cv 'charentais fom 1' carries a single dominant gene conferring resistance against fom races 0 and 2, whereas 'charentais fom 2' c ... | 2006 | 17390848 |
| antifungal activity of cymbopogon parkeri stapf. essential oil on some important phytopathogenic fungi. | the genus cymbopogon that belongs to the poaceae (graminae) has some important aromatic species whit remarkable commercial value. essential oils from different species of the cymbopogon are used in the perfumery, cosmetic and soap industries and some of them have antifungal and insecticide activity. in this study, antifungal activity of c. parkeri essential oil on the growth of rhizoctonia solani, pyricularia orizea and fusarium oxysporum, three important phytopathogenic fungi, was investigated. ... | 2006 | 17390842 |
| in vivo time-lapse documentation using confocal and multi-photon microscopy reveals the mechanisms of invasion into the arabidopsis root vascular system by fusarium oxysporum. | fusarium oxysporum, a major soil-borne fungal pathogen, causes vascular wilt, damping-off, and root rot diseases on over 100 cultivated plant species. mechanisms of root colonization by f. oxysporum in arabidopsis thaliana were studied through in planta 3-dimensional time-lapse documentation using confocal and multi-photon microscopy. data from individual encounter sites were acquired repeatedly over a several day period without physical manipulation or retrieval from the growth chamber. in vivo ... | 2007 | 17379550 |
| zirconia enrichment in zircon sand by selective fungus-mediated bioleaching of silica. | one of the important routes for the production of zirconia is by chemical treatment and removal of silica from zircon sand (zrsixoy). we present here a completely green chemistry approach toward enrichment of zirconia in zircon sand; this is based on the reaction of the fungus fusarium oxysporum with zircon sand by a process of selective extracellular bioleaching of silica nanoparticles. since this reaction does not result in zirconia being simultaneously leached out from the sand, there is a co ... | 2007 | 17375939 |
| characterization of fusarium oxysporum beta-1,6-galactanase, an enzyme that hydrolyzes larch wood arabinogalactan. | a type ii arabinogalactan-degrading enzyme (fogal1) was purified from fusarium oxysporum 12s, and the corresponding cdna was isolated. fogal1 had high similarity to enzymes of glycoside hydrolase family 5. treatment of larch wood arabinogalactan with the recombinant enzyme indicated that fogal1 is a beta-1,6-galactanase that preferentially debranches beta-1,6-galactobiose from the substrate. | 2007 | 17351093 |
| insecticidal and antifungal activity of a protein from pouteria torta seeds with lectin-like properties. | this paper describes the purification and characterization of a novel protein from the seeds of pouteria torta (family sapotaceae). the protein was purified by a combination of gel filtration, ion-exchange, and reverse phase chromatographies. sds-page of the purified protein resulted in a single protein band of 14 kda in the presence and absence of dtt. the lectin-like activity of pouterin was best inhibited by glycoproteins such as fetuin, asialofetuin, heparin, orosomucoid, and ovoalbumin. pou ... | 2007 | 17348680 |
| purification, characterization, and molecular gene cloning of an antifungal protein from ginkgo biloba seeds. | a novel basic protein with antifungal activity was isolated from the seeds of ginkgo biloba and purified to homogeneity. the protein inhibited the growth of some fungi (fusarium oxysporum, trichoderma reesei, and candida albicans) but did not exhibit antibacterial action against escherichia coli. furthermore, this protein showed weak inhibitory activity against the aspartic protease pepsin. to design primers for gene amplification, the nh(2)-terminal and partial internal amino acid sequences wer ... | 2007 | 17338634 |
| antifungal activity of aloe vera leaves. | aloe vera fresh leaves hydroalcoholic plant extract was tested against the mycelial growth of botrytis gladiolorum, fusarium oxysporum f.sp. gladioli, heterosporium pruneti and penicillium gladioli on czapek-agar medium. the minimum fungicidal concentration (mfc) varied between 80 and 100 microl/ml, depending on the fungal species. | 2007 | 17336466 |
| biocontrol and other beneficial activities of bacillus subtilis isolated from cowdung microflora. | bacillus subtilis strains isolated from cowdung (cd) had several beneficial attributes, which included biocontrol, plant growth promotion, sulphur (s) oxidation, phosphorus (p) solubilization and production of industrially important enzymes (amylase and cellulase). the b. subtilis strains from cd inhibited the in vitro growth of fungi, fusarium oxysporum (25-34%) and botryodiplodia theobromae (100%), isolated from the postharvest rots of yam (dioscorea rotundata) tubers. other than biocontrol, b ... | 2009 | 17320363 |
| some fungal endophytes from vegetable crops and their anti-oomycete activities against tomato late blight. | to isolate endophytic fungi from vegetable plants and examine their in vivo anti-oomycete activity against phytophthora infestans in tomato plants. | 2007 | 17309513 |
| a thaumatin-like antifungal protein from the emperor banana. | a 20-kda protein with substantial n-terminal sequence homology to thaumatin-like proteins was isolated from ripe fruits of the emperor banana, musa basjoo cv. 'emperor banana'. the isolation procedure entailed (nh(4))(2)so(4) precipitation, ion exchange chromatography on deae-cellulose, and affinity chromatography on affi-gel blue gel. the thaumatin-like protein inhibited mycelial growth in fusarium oxysporum and mycosphaerella arachidicola. however, it did not affect the mitogenic response of m ... | 2007 | 17306420 |
| fow2, a zn(ii)2cys6-type transcription regulator, controls plant infection of the vascular wilt fungus fusarium oxysporum. | the filamentous fungus fusarium oxysporum is a soil-borne parasite that causes vascular wilts in a wide variety of crops by directly penetrating roots and colonizing the vascular tissue. in previous work, we generated the non-pathogenic mutant b137 of the melon wilt pathogen f. oxysporum f. sp. melonis by using restriction enzyme-mediated integration (remi) mutagenesis. molecular characterization of b137 revealed that this mutant has a single-copy plasmid insertion in a gene, designated fow2, wh ... | 2007 | 17302801 |
| characterization of a lectin from gonatanthus pumilus d. don having anti-proliferative effect against human cancer cell lines. | a monocot araceous lectin from tubers of gonatanthus pumilus (gpl) wa earlier purified in our laboratory and reported as t-cell mitogen having homotetrameric structure with subunit molecular mass of 13 kda. besides asialofetuin as reported earlier, in the present study it was also inhibited by n-acetyl-d-lactosamine but was non-reactive towards mannose or its derivatives. the lectin is rich in acidic amino acids and cysteine is completely absent. chemical modification of gpl revealed requirement ... | 2007 | 17288049 |
| search for cell motility and angiogenesis inhibitors with potential anticancer activity: beauvericin and other constituents of two endophytic strains of fusarium oxysporum. | wound-healing assay-guided fractionation of an etoac extract of the fungal strain fusarium oxysporum eph2raa endophytic in ephedra fasciculata afforded beauvericin (1), (-)-oxysporidinone (2), and two new n-methyl-2-pyridones, (-)-4,6'-anhydrooxysporidinone (3) and (-)-6-deoxyoxysporidinone (4). beauvericin (1) inhibited migration of the metastatic prostate cancer (pc-3m) and breast cancer (mda-mb-231) cells and showed antiangiogenic activity in huvec-2 cells at sublethal concentrations. cytotox ... | 2007 | 17286429 |
| lignin deficiency in transgenic flax resulted in plants with improved mechanical properties. | flax (linum usitatissimum l.) is a very important source of natural fibres used by the textile industry. flax fibres are called lignocellulosic, because they contain mainly cellulose (about 70%), with hemicellulose, pectin and lignin. lignin is a three-dimensional polymer with a high molecular weight, and it gives rigidity and mechanical resistance to the fibre and plant. its presence means the fibres have worse elastic properties than non-lignocellulosic fibres, e.g. cotton fibres, which contai ... | 2007 | 17280732 |
| the antifungal protein afp from aspergillus giganteus inhibits chitin synthesis in sensitive fungi. | the antifungal protein afp from aspergillus giganteus is highly effective in restricting the growth of major human- and plant-pathogenic filamentous fungi. however, a fundamental prerequisite for the use of afp as an antifungal drug is a complete understanding of its mode of action. in this study, we performed several analyses focusing on the assumption that the chitin biosynthesis of sensitive fungi is targeted by afp. here we show that the n-terminal domain of afp (amino acids 1 to 33) is suff ... | 2007 | 17277210 |
| selection of bacteria able to control fusarium oxysporum f. sp. radicis-lycopersici in stonewool substrate. | tomato foot and root rot (tfrr), caused by fusariumoxysporum f. sp. radicis-lycopersici (forl), is an economically important disease of tomato. the aim of this study was to develop an efficient protocol for the isolation of bacteria, which controls tfrr based on selection of enhanced competitive root-colonizing bacteria from total rhizosphere soil samples. | 2007 | 17241352 |
| voriconazole and fungal keratitis: a report of two treatment failures. | two cases of fungal keratitis, one caused by fusarium oxysporum and the other by colletotrichum dematium, that failed treatment with topical voriconazole 1% are presented. these cases are noteworthy in that topical voriconazole was unable to eradicate the corneal infection in both cases, despite relatively low mic90 tested for each species of 8 and 1 microg/ml, respectively. | 2006 | 17238810 |
| in vitro antimycotic activity of xanthorrhizol isolated from curcuma xanthorrhiza roxb. against opportunistic filamentous fungi. | xanthorrhizol was isolated from the rhizome of curcuma xanthorrhiza (zingiberaceae) and its in vitro activity against opportunistic filamentous fungi was evaluated using the nccls (m38-a) standard method. xanthorrhizol was found to be active against all the species tested, namely aspergillus flavus, aspergillus fumigatus, aspergillus niger, fusarium oxysporum, rhizopus oryzae and trichophyton mentagrophytes: the mics being 2.0, 2.0, 2.0, 4.0, 1.0 and 1.0 microg/ml, while the mfcs were 4.0, 4.0, ... | 2007 | 17236167 |
| partial silencing of a hydroxy-methylglutaryl-coa reductase-encoding gene in trichoderma harzianum cect 2413 results in a lower level of resistance to lovastatin and lower antifungal activity. | in the present article, we describe the cloning and characterization of the trichoderma harzianum hmgr gene encoding a hydroxymethylglutaryl coa reductase (hmgr), a key enzyme in the biosynthesis of terpene compounds. in t. harzianum, partial silencing of the hmgr gene gave rise to transformants with a higher level of sensitivity to lovastatin, a competitive inhibitor of the hmgr enzyme. in addition, these hmgr-silenced transformants produced lower levels of ergosterol than the wild-type strain ... | 2007 | 17218128 |
| modeling competition for infection sites on roots by nonpathogenic strains of fusarium oxysporum. | by use of plane and solid geometry and probability models, efficiencies of infection and competition for nutrients and infection sites by a nonpathogenic strain of fusarium oxysporum (c14) with f. oxysporum f. sp. cucumerinum on the rhizoplane of cucumber were calculated. the model is derived from previously published data. efficiencies for successful infection were 0.04 chlamydospores per infection site for both pathogen and nonpathogen. observed successful infections by the pathogen in competi ... | 2007 | 17216327 |
| solid-state cultures of fusarium oxysporum transform aromatic components of olive-mill dry residue and reduce its phytotoxicity. | the present study mainly investigated the ability of solid-state cultures of the non-pathogenic fusarium oxysporum strain bafc 738 to transform aromatic components to reduce the phytotoxicity in olive-mill dry residue (dor), the waste from the two-phase manufacturing process. lignin, hemicellulose, fats and water-soluble extractives contents of dor colonized by the fungus for 20 weeks were reduced by 16%, 25%, 71% and 13%, respectively, while the cellulose content increased by 25%. in addition, ... | 2007 | 17207620 |
| comparative essential oil composition and antifungal effect of bitter fennel (foeniculum vulgare ssp. piperitum) fruit oils obtained during different vegetation. | the chemical composition of the flower and unripe and ripe fruits from fennel (bitter) (foeniculum vulgare ssp. piperitum) has been examined by gas chromatography and gas chromatography-mass spectrometry. the main identified components of the flower and unripe and ripe fruit oils were estragole (53.08%, 56.11%, and 61.08%), fenchone (13.53%, 19.18%, and 23.46%), and alpha-phellandrene (5.77%, 3.30%, and 0.72%), respectively. minor qualitative and major quantitative variations for some compounds ... | 2006 | 17201644 |
| [evaluation of cellular response in engorged females of boophilus microplus (canestrini, 1887) inoculated with metarhizium anisopliae, beauveria bassiana, penicillium corylophilum or fusarium oxysporum]. | the effect of beauveria bassiana, metarhizium anisopliae, penicillium corylophilum or fusarium oxysporum on the dynamic of hemocytes presented in the haemolymph of engorged females of boophilus microplus was studied. the inoculation was carried out with conidia suspension of different fungi in the concentration of 10(8) conidia/ml. a negative control group was inoculated with 0.1% tween 80 water solution and a testimony group was comprised of non inoculated ticks. the haemolymph samples were col ... | 2006 | 17196118 |
| extracellular biosynthesis of magnetite using fungi. | the development of synthetic processes for oxide nanomaterials is an issue of considerable topical interest. while a number of chemical methods are available and are extensively used, the collaborations are often energy intensive and employ toxic chemicals. on the other hand, the synthesis of inorganic materials by biological systems is characterized by processes that occur at close to ambient temperatures and pressures, and at neutral ph (examples include magnetotactic bacteria, diatoms, and s- ... | 2006 | 17193569 |
| enhanced degradation and toxicity reduction of dihexyl phthalate by fusarium oxysporum f. sp. pisi cutinase. | this research aims to investigate the efficiency of two lipolytic enzymes--fungal cutinase and yeast esterase--upon the biodegradation of dihexyl phthalate (dhp). | 2007 | 17184338 |
| transposition of a fungal miniature inverted-repeat transposable element through the action of a tc1-like transposase. | the mimp1 element previously identified in the ascomycete fungus fusarium oxysporum has hallmarks of miniature inverted-repeat transposable elements (mites): short size, terminal inverted repeats (tirs), structural homogeneity, and a stable secondary structure. since mimp1 has no coding capacity, its mobilization requires a transposase-encoding element. on the basis of the similarity of tirs and target-site preference with the autonomous tc1-like element impala, together with a correlated distri ... | 2007 | 17179071 |
| isolation and characterization of an antifungal peptide with antiproliferative activity from seeds of phaseolus vulgaris cv. 'spotted bean'. | a 7.3-kda antifungal peptide with an n-terminal sequence exhibiting remarkable homology to defensins from other leguminous plants was isolated from phaseolus vulgaris cv. 'spotted bean'. the isolation procedure involved ion exchange chromatography on o-diethylaminoethyl (deae) cellulose, affinity chromatography on affi-gel blue gel, ion exchange chromatography on sp-sepharose, and gel filtration by fast protein liquid chromatography on superdex 75. the peptide was unadsorbed on deae-cellulose an ... | 2007 | 17177050 |
| evaluation of chenopodium ambrosioides oil as a potential source of antifungal, antiaflatoxigenic and antioxidant activity. | essential oil extracted from the leaves of chenopodium ambrosioides linn. (chenopodiaceae) was tested against the aflatoxigenic strain of test fungus aspergillus flavus link. the oil completely inhibited the mycelial growth at 100 microg/ml. the oil exhibited broad fungitoxic spectrum against aspergillus niger, aspergillus fumigatus, botryodiplodia theobromae, fusarium oxysporum, sclerotium rolfsii, macrophomina phaseolina, cladosporium cladosporioides, helminthosporium oryzae and pythium debary ... | 2007 | 17174000 |
| alteration of substrate specificity of fructosyl-amino acid oxidase from fusarium oxysporum. | fructosyl-amino acid oxidase (fod-f) from fusarium oxysporum f. sp. raphani (nbrc 9972) is the enzyme catalyzing the oxidative deglycation of fructosyl-amino acids such as n(epsilon)-fructosyl n(alpha)-benzyloxycarbonyl-lysine (fzk) and fructosyl valine (fv), which are model compounds of the glycated proteins in blood. wild-type fod-f has high activities toward both substrates. we obtained a mutant fod-f, which reacts with fzk but not with fv by random mutagenesis. one amino-acid substitution (k ... | 2007 | 17160532 |
| antimicrobial activity of catechol and pyrogallol as allelochemicals. | catechol and pyrogallol are allelochemicals which belong to phenolic compounds synthesized in plants. their antimicrobial activities were investigated on three bacteria (pseudomonas putida, pseudomonas pyocyanea, corynebacterium xerosis) and two fungi (fusarium oxysporum, penicillium italicum) phytopathogenic species as test organisms using the disc diffusion method. both catechol and pyrogallol were found to have antibacterial effects on all the bacteria used in the study at 5 and 10 mm concent ... | 2006 | 17137106 |
| comparison of the hydrolysis of polyethylene terephthalate fibers by a hydrolase from fusarium oxysporum lch i and fusarium solani f. sp. pisi. | the hydrolysis of polyethylene terephthalate (pet) fibers by two fungal hydrolases was investigated. the hydrolase from a newly isolated fusarium oxysporum strain (lch 1) was more efficient in releasing terephthalic acid from pet fibers compared to the enzyme from f. solani f. sp. pisi dsm 62420 when equal amounts of p-nitrophenyl butyrate-hydrolyzing activity were employed. pet fabrics treated under the same conditions with the enzyme from f. oxysporum lch 1 also showed a considerably higher in ... | 2007 | 17136729 |
| an antifungal peptide from red lentil seeds. | an antifungal peptide, with a molecular mass of 11 kda, was isolated from dry seeds of the red lentil (lens culinaris) using a procedure that involved four chromatographic steps. the antifungal peptide was unadsorbed on deae-cellulose, and adsorbed on affi-gel blue gel and s-sepharose. the final chromatographic step involved gel filtration by fast protein liquid chromatography on superdex 75. the antifungal peptide inhibited mycelial growth in mycosphaerella arachidicola with an ic50 of 36 micro ... | 2007 | 17123664 |
| aterf14, a member of the erf family of transcription factors, plays a nonredundant role in plant defense. | we had previously shown that several transcription factors of the ethylene (et) response factor (erf) family were induced with different but overlapping kinetics following challenge of arabidopsis (arabidopsis thaliana) with pseudomonas syringae pv tomato dc3000 (avrrpt2). one of these genes, a transcriptional activator, aterf14, was induced at the same time as erf-target genes (chib, basic chitinase). to unravel the potential function of aterf14 in regulating the plant defense response, we have ... | 2007 | 17114278 |
| activities and survival of endophytic bacteria in white clover (trifolium repens l.). | in this study, the genera, abundance, and activities of endophytic bacteria in field-grown white clover (trifolium repens) and the fate of introduced antibiotic-tolerant bacteria in white clover tissues were investigated. pseudomonas, pantoea, and corynebacterium were the most frequently isolated endophytic bacteria genera, whereas xanthomonas, microbacterium, and cellulomonas occurred less frequently. the average bacterial populations in stolons and roots were approximately 100,000 colony-formi ... | 2006 | 17110977 |
| effect of acidic electrolyzed water on the viability of bacterial and fungal plant pathogens and on bacterial spot disease of tomato. | acidic electrolyzed water (aew), known to have germicidal activity, was obtained after electrolysis of 0.045% aqueous solution of sodium chloride. freshly prepared aew (ph 2.3-2.6, oxidation-reduction potential 1007-1025 mv, and free active chlorine concentration 27-35 ppm) was tested in vitro and (or) on tomato foliage and seed surfaces for its effects on the viability of plant pathogen propagules that could be potential seed contaminants. foliar sprays of aew were tested against bacterial spot ... | 2006 | 17110959 |
| a mannose-specific tetrameric lectin with mitogenic and antibacterial activities from the ovary of a teleost, the cobia (rachycentron canadum). | a tetrameric lectin, with hemagglutinating activity toward rabbit erythrocytes and with specificity toward d-mannosamine and d(+)-mannose, was isolated from the ovaries of a teleost, the cobia rachycentron canadum. the isolation protocol comprised ion exchange chromatography on cm-cellulose and q-sepharose, ion exchange chromatography by fast protein liquid chromatography (fplc) on mono q, and finally gel filtration by fplc on superose 12. the lectin was adsorbed on all ion exchangers used. it e ... | 2007 | 17109173 |
| a novel antiproliferative and antifungal lectin from amaranthus viridis linn seeds. | a lectin from the seeds of amaranthus viridis linn has been purified by affinity chromatography on asialofetuin-linked amino activated silica. amaranthus viridis lectin (avl) has a native molecular mass of 67 kda. it is a homodimer composed of two 36.6 kda subunits. the lectin gave a single band in non-denaturing page at ph 4.5 and ph 8.3 and a single peak on hplc size exclusion and cation exchange columns. the purified lectin was specific for both t-antigen and n-acetyl-d-lactosamine, markers f ... | 2006 | 17100645 |
| structural and functional diversity within the cystatin gene family of hordeum vulgare. | phytocystatins are inhibitors of cysteine proteinases from plants putatively involved in defence and as endogenous regulators of protein turnover. seven genes encoding cystatins (hvcpi-1 to hvcpi-7), identified from est collections and from an endosperm cdna library, have been characterized. the intron-exon structure of their corresponding orfs has been determined and the predicted three-dimensional models for the seven barley cystatins have been established, based on the known crystal structure ... | 2006 | 17099080 |
| distinct signalling pathways coordinately contribute to virulence of fusarium oxysporum on mammalian hosts. | the filamentous fungus fusarium oxysporum causes vascular wilt on a wide range of plant species and is an emerging pathogen of humans. a mitogen-activated protein kinase, fmk1, and a g protein beta subunit, fgb1, control pathogenicity of f. oxysporum on plants through distinct signalling pathways. in the present report, we studied the genetic interaction between fmk1 and fgb1 and their role in virulence on a mammalian host. the delta fmk1 or delta fgb1 single mutants exhibited similar virulence ... | 2006 | 17095278 |
| biologic control ability of plant growth-promoting paenibacillus lentimorbus nrrl b-30488 isolated from milk. | a plant growth-promoting paenibacillus lentimorbus nrrl b-30488 (b-30488) was isolated from cows' milk. bacterial colonization and growth responses of different plant species after inoculation with b-30488 were evaluated in a controlled environment and in microplot assays. survival and colonization of b-30488 in the phytosphere of plants and soil was monitored using a chromosomally located rifampicin-marked mutant b-30488 (b-30488r). the strain showed variable ability to invade plants. the inter ... | 2006 | 17089220 |
| rhizobium etli usda9032 engineered to produce a phenazine antibiotic inhibits the growth of fungal pathogens but is impaired in symbiotic performance. | phenazine production was engineered in rhizobium etli usda9032 by the introduction of the phz locus of pseudomonas chlororaphis o6. phenazine-producing r. etli was able to inhibit the growth of botrytis cinerea and fusarium oxysporum in vitro. black bean inoculated with phenazine-producing r. etli produced brownish fix(-) nodules. | 2007 | 17085692 |
| fungus-mediated biotransformation of amorphous silica in rice husk to nanocrystalline silica. | rice husk is a cheap agro-based waste material, which harbors a substantial amount of silica in the form of amorphous hydrated silica grains. however, there have been no attempts at harnessing the enormous amount of amorphous silica present in rice husk and its room-temperature biotransformation into crystalline silica nanoparticles. in this study, we address this issue and describe how naturally deposited amorphous biosilica in rice husk can be bioleached and simultaneously biotransformed into ... | 2006 | 17061888 |
| filamentous fungi (magnaporthe grisea and fusarium oxysporum). | a better understanding of fungal biology will facilitate judicious use of beneficial fungi and will also advance our efforts to control pathogenic fungi. molecular studies of fungal biology have been greatly aided by transformation-mediated mutagenesis techniques. transformation via nonhomologous integration of plasmid dna bearing a selectable marker (e.g., antibiotic resistance gene) has been widely used for the random insertional mutagenesis of fungi - as an alternative to chemical and radiati ... | 2006 | 17033082 |
| bacterial expression of a trichosanthes kirilowii defensin (tdef1) and its antifungal activity on fusarium oxysporum. | the gene encoding trichosanthes kirilowii defensin (tdef1) was cloned by reverse transcriptase-polymerase chain reaction (rt-pcr). the newly discovered tdef1 cdna contains 231 bp (genbank accession number dq526373) and encodes a 76-amino acid protein, which consists of a 29-amino acid signal peptide and a 47-amino acid mature peptide. the partial cdna, corresponding to the mature peptide coding region of tdef1, was inserted into bacterial expression vector pet32a(+). subsequent expression showed ... | 2007 | 17024471 |
| effects of the tomato pathogen fusarium oxysporum f. sp. radicis-lycopersici and of the biocontrol bacterium pseudomonas fluorescens wcs365 on the composition of organic acids and sugars in tomato root exudate. | the effects of the pathogenic fungus fusarium oxysporum f. sp. radicis-lycopersici and of the bacterial biocontrol strain pseudomonas fluorescens wcs365, and of both microbes, on the amounts and composition of root exudate components of tomato plants grown in a gnotobiotic stonewool substrate system were studied. conditions were selected under which introduction of f. oxysporum f. sp. radicis-lycopersici caused severe foot and root rot, whereas inoculation of the seed with p. fluorescens wcs365 ... | 2006 | 17022176 |
| screening for anti-infective properties of several medicinal plants of the mauritians flora. | several plants of the mauritian flora alleged to possess anti-infective properties were studied against different strains of pathogenic bacteria and fungi. the grounded dried plant materials were extracted with different extractants and screened for anti-microbial activity using the disk diffusion and the micro-dilution techniques. preliminary screening revealed that the methanol extracts were most active. salmonella enteritidis, enterobacter cloacae and bacillus subtilis were the three test org ... | 2007 | 17011733 |
| antimicrobial properties of a non-toxic glycoprotein (wsg) from withania somnifera (ashwagandha). | a monomeric glycoprotein with a molecular mass of 28 kda in sds-page was isolated from the withania somnifera root tubers. the protein designated wsg (withania somnifera glycoprotein) demonstrated potent antimicrobial activity against the phytopathogenic fungi and bacteria tested. antifungal effect has been demonstrated in that wsg exerts a fungistastic effect by inhibiting spore germination and hyphal growth in the tested fungi. wsg showed potent antifungal activity against aspergillus flavus, ... | 2006 | 17009292 |
| phytochemical and antimicrobial study of an antidiabetic plant: scoparia dulcis l. | the antimicrobial and antifungal effects of different concentrations of chloroform/methanol fractions of scoparia dulcis were investigated. the isolated fractions were tested against different bacteria like salmonella typhii, staphylococcus aureus, escherichia coli, bacillus subtilis, pseudomonas aeruginosa, and proteus vulgaris and fungal strains such as alternaria macrospora, candida albicans, aspergillus niger, and fusarium oxysporum. the isolated fractions exhibited significant antimicrobial ... | 2006 | 17004904 |
| pip, a novel activator of phenazine biosynthesis in pseudomonas chlororaphis pcl1391. | secondary metabolites are important factors for interactions between bacteria and other organisms. pseudomonas chlororaphis pcl1391 produces the antifungal secondary metabolite phenazine-1-carboxamide (pcn) that inhibits growth of fusarium oxysporum f. sp. radius lycopersici the causative agent of tomato foot and root rot. our previous work unraveled a cascade of genes regulating the pcn biosynthesis operon, phzabcdefgh. via a genetic screen, we identify in this study a novel tetr/acrr regulator ... | 2006 | 16997957 |
| antimicrobial activity of extracts of chemical races of the lichen pseudevernia furfuracea and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents. | the antimicrobial activity and the mic values of the ethanol, chloroform, diethyl ether, and acetone extracts of the chemical races of pseudevernia furfuracea (var. furfuracea and var. ceratea) and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents have been investigated against some microorganisms. nearly all extracts of both chemical races showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, prote ... | 2006 | 16989308 |
| influence of glyphosate on rhizoctonia and fusarium root rot in sugar beet. | this study tests the effect of glyphosate application on disease severity in glyphosate-resistant sugar beet, and examines whether the increase in disease is fungal or plant mediated. in greenhouse studies of glyphosate-resistant sugar beet, increased disease severity was observed following glyphosate application and inoculation with certain isolates of rhizoctonia solani kuhn and fusarium oxysporum schlecht. f. sp. betae snyd. & hans. significant increases in disease severity were noted for r. ... | 2006 | 16988927 |
| isolation and characterization of a novel thermostable non-specific lipid transfer protein-like antimicrobial protein from motherwort (leonurus japonicus houtt) seeds. | in screening for potent antimicrobial proteins from plant seeds, a novel heat-stable antimicrobial protein, designated ljamp2, was purified from seeds of the motherwort (leonurus japonicus houtt), a medicine herb, with a procedure involving cation exchange chromatography on a cm ff column, and reverse phase hplcs on c8 column and c18 column. ljamp2 exhibited a molecular mass of 6.2 kda determined. automated edman degradation determined the partial n-terminal sequence of ljamp2 to be nh2-aigcntva ... | 2006 | 16979797 |