Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| effect of poly(vinyl acetate-acrylamide) microspheres properties and steric hindrance on the immobilization of candida rugosa lipase. | poly(vinyl acetate-acrylamide) microspheres were synthesized in the absence or presence of isooctane via suspension polymerization and utilized as carriers to immobilize candida rugosa lipase. when the hydrophobic/hydrophilic surface characteristics of the microspheres were modified by changing the ratio of vinyl acetate (hydrophobic monomer) to acrylamide (hydrophilic monomer) from 50:50 to 86:24, the immobilization ratio changed from 45% to 92% and the activity of the immobilized lipase increa ... | 2012 | 22989650 |
| preparation of superparamagnetic fe3o4@alginate/chitosan nanospheres for candida rugosa lipase immobilization and utilization of layer-by-layer assembly to enhance the stability of immobilized lipase. | superparamagnetic alginate nanospheres with diameter of 50 nm were prepared by self-assembly of alginate in the ca(2+) solution; and then superparamagnetic alginate/chitosan nanospheres, which have positive charge and could adsorb lipase directly, were obtained with a following assembly of chitosan based on the electrostatic interaction between alginate and chitosan. subsequently, oxidic poly (ethylene glycol) was used to functionalize the magnetic alginate/chitosan nanospheres. thus, the magnet ... | 2012 | 22985256 |
| biodiversity and probiotic potential of yeasts isolated from fura, a west african spontaneously fermented cereal. | fura is a spontaneously fermented pearl millet product consumed in west africa. the yeast species involved in the fermentation were identified by pheno- and genotypic methods to be candida krusei, kluyveromyces marxianus, candida tropicalis, candida rugosa, candida fabianii, candida norvegensis and trichosporon asahii. c. krusei and k. marxianus were found to be the dominant species. survival in ph 2.5 or in the presence of bile salts (0.3% (w/v) oxgall) and growth at 37°c were independently det ... | 2012 | 23072700 |
| role of plasmid in diesel oil degradation by yeast species isolated from petroleum hydrocarbon-contaminated soil. | five yeast species, namely candida tropicalis, cryptococcus laurentii, trichosporon asahii, rhodotorula mucilaginosa and candida rugosa isolated from hydrocarbon-contaminated soil were found to be potent degraders of diesel oil. these microorganisms showed the presence of enzymes cytochrome p450, nadph cytochrome c reductase, aminopyrine n demethylase, alcohol dehydrogenase, aldehyde dehydrogenase, naphthalene dioxygenase, catalase and glutathione s transferase when the cells were incubated for ... | 2012 | 22629639 |
| rapid identification of yeast isolates from clinical specimens in critically ill trauma icu patients. | the purpose was to evaluate the performance of a commercially available chromogenic candida speciation media and the vitek 2 id system for the identification of medically important yeasts and yeast-like organisms in a routine clinical microbiology laboratory. | 2012 | 22923919 |
| promotion of the lipase-catalyzed hydrolysis of conjugated linoleic acid l-menthyl ester by addition of an organic solvent. | conjugated linoleic acid l-menthyl ester was hydrolyzed in water by the lipase from candida rugosa with the addition of an organic solvent. the degree of hydrolysis (yield) greatly improved when a tertiary alcohol, such as t-butyl alcohol, was added. however, the addition of a less polar solvent, such as hexane, decreased the degree of hydrolysis, and some water-miscible solvents, such as acetone, caused inactivation of the lipase. with the addition of t-butyl alcohol, the reaction mixture forme ... | 2012 | 23420098 |
| lipase-immobilized biocatalytic membranes for biodiesel production. | microbial lipase from candida rugosa (amano ay-30) has good transesterification activity and can be used for biodiesel production. in this study, polyvinylidene fluoride (pvdf) membrane was grafted with 1,4-diaminobutane and activated by glutaraldehyde for c. rugosa lipase immobilization. after immobilization, the biocatalytic membrane was used for producing biodiesel from soybean oil and methanol via transesterification. response surface methodology (rsm) in combination with a 5-level-5-factor ... | 2012 | 23357586 |
| in silico and experimental characterization of chimeric bacillus thermocatenulatus lipase with the complete conserved pentapeptide of candida rugosa lipase. | lipases are one of the highest value commercial enzymes as they have broad applications in detergent, food, pharmaceutical, and dairy industries. to provide chimeric bacillus thermocatenulatus lipase (btl2), the completely conserved pentapeptide (¹¹²ala-his-ser-gln-gly¹¹⁶) was replaced with similar sequences (²⁰⁷gly-glu-ser-ala-gly²¹¹) of candida rugosa lipase (clr) at the nucleophilic elbow region. for this purpose, three mutations including a112g, h113e, and q115a were inserted in the conserve ... | 2012 | 23274720 |
| biochemical methane potential of raw and pre-treated meat-processing wastes. | raw and pre-treated greaves and rinds, two meat-processing wastes, were assessed for biochemical methane potential (bmp). combinations of temperature (25, 55, 70 and 120 °c), naoh (0.3 g g(-1) waste volatile solids) and lipase from candida rugosa (10 u g(-1) fat) were applied to promote wastes hydrolysis, and the effect on bmp was evaluated. cod solubilisation was higher (66% for greaves; 55% for rinds) when greaves were pre-treated with naoh at 55 °c and lipase was added to rinds after autoclav ... | 2012 | 23266854 |
| immobilization of candida rugosa lipase on poly(3-hydroxybutyrate-co-hydroxyvalerate): a new eco-friendly support. | the overall objective of this study is to evaluate the morphological [scanning electron microscopy (sem)], physicochemical [differential scanning calorimetry (dsc), thermogravimetric analysis (tga), chemical composition analysis, fourier-transform infrared spectroscopy (ftir), nuclear magnetic resonance (nmr)], and biochemical properties of candida rugosa lipase (crl) immobilized on a natural biopolymer poly(3-hydroxybutyrate-co-hydroxyvalerate) (phbv) in aqueous solution. crl was immobilized by ... | 2011 | 21870100 |
| mechanistic investigations of lipase-catalyzed degradation of polycarbonate in organic solvents. | the biodegradation of an engineering thermoplastic, poly (bisphenol-a carbonate) (bpapc), was carried out using three different lipases from candida antarctica (cal), candida rugosa (crl) and porcine pancreas (ppl) in water-miscible (tetrahydrofuran) and water-immiscible (chloroform) solvents for 10 days. the degradation was monitored by gel permeation chromatography and fourier transform infrared spectroscopy. maximum degradation (ca. 60% reduction in m(n)) of bpapc was observed in thf with ppl ... | 2011 | 22112773 |
| conformational flexibility of lipase lip1 from candida rugosa studied by electronic spectroscopies and thermodynamic approaches. | we have used second-order orthogonal designs to obtain empirical models that describe the combined effect of ph and temperature on the secondary structure of a lipase (lip1) from candida rusosa. the equations that describe lipase conformational flexibility were derivated from the enzyme alpha helix fraction obtained from the experimental matrix. the thermal unfolding of lipase at different ph values was followed by measuring the circular dichroism signal as a function of temperature over a tempe ... | 2011 | 21318273 |
| candida rugosa: a new fungal pathogen emerging, but from where? | 2011 | 21212147 | |
| mechanism of acetaldehyde-induced deactivation of microbial lipases. | microbial lipases represent the most important class of biocatalysts used for a wealth of applications in organic synthesis. an often applied reaction is the lipase-catalyzed transesterification of vinyl esters and alcohols resulting in the formation of acetaldehyde which is known to deactivate microbial lipases, presumably by structural changes caused by initial schiff-base formation at solvent accessible lysine residues. previous studies showed that several lipases were sensitive toward acetal ... | 2011 | 21342514 |
| immobilization of lipase from candida rugosa on sepabeads(®): the effect of lipase oxidation by periodates. | the objective of this paper was the investigation of a suitable sepabeads(®) support and method for immobilization of lipase from candida rugosa. three different supports were used, two with amino groups, (sepabeads(®) ec-ea and sepabeads(®) ec-ha), differing in spacer length (two and six carbons, respectively) and one with epoxy group (sepabeads(®) ec-ep). lipase immobilization was carried out by two conventional methods (via epoxy groups and via glutaraldehyde), and with periodate method for m ... | 2011 | 21347667 |
| immobilization of candida rugosa lipase on hydrophobic/strong cation-exchange functional silica particles for biocatalytic synthesis of phytosterol esters. | in this work, mixed-mode silica particles functionalized with octyl and sulfonic acid groups was conveniently prepared by co-bonding a mixture of n-octyltriethoxysilane and 3-mercaptopropyltriethoxysilane and then oxidized with hydrogen peroxide. candida rugosa lipase (crl) was immobilized on the mixed-mode silica particles via hydrophobic and strong cation-exchange interaction. the resulting immobilized crl increased remarkably its stability at high temperature in comparison to free crl. the im ... | 2011 | 22209442 |
| phyto-inspired silica nanowires: characterization and application in lipase immobilization. | silica nanostructures were phyto-fabricated on different surfaces by using pomegranate (punica granatum) leaf extracts. on zinc films, nanowires were obtained. on other surfaces such as silica, alumina, zinc oxide and glass, spherical aggregates, cubic assemblies, micro-flakes and acicular structures, respectively, were observed. the nanowires developed on zn surfaces were characterized by scanning electron microscope-energy dispersive spectrometer (sem-eds), x-ray diffraction (xrd), photolumin ... | 2011 | 22201456 |
| chemoenzymatic synthesis with lipase catalyzed resolution and evaluation of antitumor activity of (r/s)-2-[2-hydroxy-3-(4-phenylpiperazin-1-yl)propyl]-1h-pyrrolo[3,4-b]quinolin-3(2h)-one. | synthesis, characterization, resolution and evaluation of novel (r/s)-2-[2-hydroxy-3-(4-phenylpiperazin-1-yl)propyl]-1h-pyrrolo[3,4-b]quinolin-3(2h)-one derivatives are described. enantiomerically pure compounds were isolated in good to excellent yield with high enantiomeric excess under mild reaction conditions by using candida antarctica b (cal-b) and candida rugosa (crl) lipases. newly synthesized and resolved compounds were screened for their antitumor activity against cancer cells such as h ... | 2011 | 21440337 |
| c-terminal region of candida rugosa lipases affects enzyme activity and interfacial activation. | candida rugosa contains several lipase (crls) genes, and crls show diverse enzyme activity despite being highly homologous across their entire protein family. previous studies found that lip4 has a high esterase activity and a low lipolytic activity and lacks interfacial activation. to investigate whether the c-terminal region of the crls mediates enzymatic activity, chimeras were generated in which the c-terminus of lip4 from either residue 374, 396, 417, or 444 to residue 534 was swapped with ... | 2011 | 21504227 |
| engineering the expression and biochemical characteristics of recombinant candida rugosa lip2 lipase by removing the additional n-terminal peptide and regional codon optimization. | candida rugosa lipase (crl), an important industrial enzyme, has been established, containing several different isoforms which were encoded by the high-identity lip gene family (lip1 to lip7). in this study, we compared the expression and biochemical characterization with three different engineered lip2 constructions in the yeast pichia pastoris. our results showed that lip2 (lip2) has an overall improvement of 50% higher production yield (1.446 u/ml) relative to that of nflip2 (0.964 u/ml) at 7 ... | 2011 | 21561168 |
| enantioselectivity of candida rugosa lipases (lip1, lip3, and lip4) towards 2-bromo phenylacetic acid octyl esters controlled by a single amino acid. | enantiomer discrimination by enzymes is a very accurate mechanism, which often involves few amino acids located at the active site. lipase isoforms from candida rugosa are very good enzymatic models to study this phenomenon as they display high sequence homology (>80%) and their enantioselectivity is often pointed out. in the present work, we investigated three lipases from c. rugosa (lip1, lip3, and lip4, respectively) towards the resolution of 2-bromo-arylacetic acid esters, an important class ... | 2011 | 21391204 |
| lipase-catalyzed synthesis of hydrophobically modified dextrans: activity and regioselectivity of lipase from candida rugosa. | vinyl decanoate-modified dextran macromolecules (dext40-vd) were synthesized in dimethyl sulfoxide at 50°c using lipase ay from candida rugosa for catalyzing transesterification between polysaccharide and vinyl fatty esters. the extent of dextran modification (quantified by the molar ratio of attached alkyl tails to sugar repeat units) with native-, ph-adjusted-, 18-crown-6 ether pretreated ph-adjusted-, and stepwise addition of pretreated lipase ay yielded <3%, 49%, 64% and 96% modified dextran ... | 2011 | 21602099 |
| cloning and transcription analysis of the candida rugosa propionyl-coa dehydrogenase gene and its expression in pichia pastoris. | the propionyl-coa dehydrogenase (pacd) gene was cloned from candida rugosa by the cdna race technique. the full cdna of the pacd gene has a length of 1408 bp, which contains a complete open reading frame (orf) of 1329 bp, coding for 442 amino acids. the cdna of pacd was cloned into the expression plasmid ppic9k and transformed into pichia pastoris gs115. the recombinant protein was purified by ni-nta affinity chromatography, and its size was observed to be approximately 49 kda as estimated by sd ... | 2011 | 21780146 |
| improvement of catalytic activity of lipase from candida rugosa via sol-gel encapsulation in the presence of calix(aza)crown. | lipase from candida rugosa (crl) was encapsulated within a chemically inert sol-gel support in the presence of calix(aza)crowns as the new additives. the catalytic activity of the encapsulated lipases was evaluated both in the hydrolysis of p-nitrophenyl palmitate (p-npp) and the enantioselective hydrolysis of racemic naproxen methyl ester. it has been observed that the percent activity yields of the calix(aza)crown based encapsulated lipases were higher than that of the free lipase. improved en ... | 2011 | 21256747 |
| intracellular characterization of aerobic glucose metabolism in seven yeast species by 13c flux analysis and metabolomics. | key distinguishing characteristics of yeast glucose metabolism are the relative proportions of fermentation and respiration. crabtree-positive yeast species exhibit a respirofermentative metabolism, whereas aerobic species respire fully without secretion of fermentation byproducts. physiological data suggest a gradual transition in different species between these two states. here, we investigate whether this gradual transition also occurs at the intracellular level by quantifying the intracellul ... | 2011 | 21205161 |
| phenotypic and genotypic characterisation of two closely related subgroups of candida rugosa in the clinical specimens. | in this study, six clinical isolates (two from blood, two from urine, and one each from bronchoalveolar lavage and vaginal swab) were identified as candida rugosa based on carbohydrate assimilation profiles by api 20c aux and id32 kits. sequence analysis of the d1/d2 domain of the yeasts differentiated the isolates into two subgroups, a and b (three isolates per subgroup) which are closely related (99.1-99.6% similarity) to c. rugosa atcc 10571 strain. comparing to c. rugosa type strain, the its ... | 2011 | 21700741 |
| enhancement of activity and selectivity of candida rugosa lipase and candida antarctica lipase a by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil. | candida rugosa lipase (crl) and candida antarctica lipase a (cala) with improved activity and selectivity were prepared for use in organic solvent media. crl bioimprinted with fatty acids exhibited eightfold enhanced transesterification activity in hexane. combination of bioimprinting and coating with lecithin or with immobilization did not improve the activity further. cala was immobilized with and without bioimprinting, none of which improved the activity. all modified lipases were tested for ... | 2011 | 21695486 |
| expression and characterization of a novel propionyl-coa dehydrogenase gene from candida rugosa in pichia pastoris. | the propionyl-coa dehydrogenase (pacd) gene was firstly cloned from candida rugosa by the cdna race technique. the 6× his-tagged recombinant pacd gene was expressed in pichia pastoris gs115 and purified with ni-nta affinity chromatography. sds-page analysis and western blotting revealed that the molecular mass of the purified pacd was 49 kda. the results showed that the recombinant protein had the activity of catalyzing propionyl-coa to acrylyl-coa. the k (m), k (cat), and v (max) values of the ... | 2011 | 21960276 |
| Lipases are soluble and active in glycerol carbonate as a novel biosolvent. | Glycerol carbonate was synthesized as biosolvent for the development of soluble enzymatic system. The effects of various reaction parameters on activity and stability of lipases were investigated using the transesterification of ethyl butyrate with n-butanol as a model reaction. Enzymatic activity in glycerol carbonate was compared with that in water and in conventional organic solvents with different ionizing and dissociating abilities. The pK(a) value of trichloroacetic acid and transesterific ... | 2011 | 22112404 |
| Assessment of the Morphological, Biochemical, and Kinetic Properties for Candida rugosa Lipase Immobilized on Hydrous Niobium Oxide to Be Used in the Biodiesel Synthesis. | Lipase from Candida rugosa (CRL) was immobilized by covalent attachment on hydrous niobium oxide. The matrix could effectively be attached to the enzyme with high retention of activity and prevent its leakage. Following immobilization, CRL exhibited improved storage stability and performed better at higher incubation temperatures. In addition, the enzyme retained most of its catalytic efficiency after successive operational cycles. The immobilized derivative was also fully characterized with res ... | 2011 | 21876790 |
| identification of local clinical candida isolates using chromagar candida™ as a primary identification method for various candida species. | the objective of our study was to study the effectiveness of chromagar candida™ as the primary identification method for various clinical candida isolates, other than the three suggested species by the manufacturer. we studied 34 clinical isolates which were isolated from patients in a local teaching hospital and 7 atcc strains. these strains were first cultured in sabouraud dextrose broth (sdb) for 36 hours at 35ºc, then on chromagar plates at 30ºc, 35ºc and 37ºc. the sensitivity of t ... | 2011 | 22041745 |
| chemoenzymatic synthesis of phytosteryl ferulates and evaluation of their antioxidant activity. | the feasibility of a two-step chemoenzymatic synthesis of phytosteryl ferulates was successfully established in this work. an intermediate vinyl ferulate was first chemically produced and subsequently esterified with phytosterols through alcoholysis with candida rugosa as a catalyst. the structures of phytosteryl ferulates were confirmed by fourier transform infrared (ftir) and high-performance chromatography-mass spectrometry/mass spectrometry (hplc-ms/ms) using atmospheric pressure chemical ... | 2011 | 22023159 |
| short communication: epidemiology and genotyping of candida rugosa strains responsible for persistent intramammary infections in dairy cows. | the present study was undertaken during an outbreak of clinical and subclinical mastitis in 14 dairy cows caused by candida rugosa, in which high somatic cell counts were seen and cases did not respond to antibiotic treatment. intramammary infection cured spontaneously in 10 cows, whereas 4 cows were culled as a result of persistent infections. repeated sampling of these cows and biomolecular analysis of the isolates showed that the infections were caused by the same genotype, even over a period ... | 2011 | 21854930 |
| enantioselective enzymatic hydrolysis of racemic drugs by encapsulation in sol-gel magnetic sporopollenin. | candida rugosa lipase was encapsulated within a sol-gel procedure and improved considerably by fluoride-catalyzed hydrolysis of mixtures of octyltriethoxysilane and tetraethoxysilane in the presence of magnetic sporopollenin. the catalytic properties of the immobilized lipases were evaluated into model reactions, i.e., the hydrolysis of p-nitrophenylpalmitate (p-npp), and the enantioselective hydrolysis of racemic naproxen methyl ester, mandelic acid methyl ester or 2-phenoxypropionic acid methy ... | 2011 | 21932062 |
| characterization of codon-optimized recombinant candida rugosa lipase 5 (lip5). | recombinant candida rugosa lipase 5 (lip5) has been functionally expressed along with other isoforms in our laboratory. however, the characterization and codon optimization of lip5 have not been done. in this work, we characterized, codon-optimized and compared lip5 with commercial lipase. lip5 activity on hydrolysis of p-nitrophenyl (p-np) butyrate was optimal at 55 °c as compared with 37 °c of the commercial lipase. several assays were also performed to determine the substrate specificity of l ... | 2011 | 21854055 |
| novel magnetic microspheres of p (gma-b-hema): preparation, lipase immobilization and enzymatic activity in two phases. | magnetic oleic-acid-coated fe(3)o(4) nanoparticles were first introduced into 1, 1-diphenylethylene (dpe)-controlled radical polymerization system to prepare superparamagnetic microspheres for enzyme immobilization by two steps of polymerization. in the presence of dpe, glycidyl methacrylate, 2-hydroxyethyl methacrylate and methacryloxyethyl trimethyl ammonium chloride with charge were selected as copolymering monomers based on their reactive functional group and excellent biocompatibility which ... | 2011 | 22159608 |
| A novel control of enzymatic enantioselectivity through the racemic temperature influenced by reaction media. | The influence of reaction media on the racemic temperature (T(r)) in the lipase-catalyzed resolution of ketoprofen vinyl ester was investigated. An effective approach to the control of the enzymatic enantioselectivity and the prediction of the increasing tendency was developed based on the T(r) influenced by reaction media. The T(r) for the resolution catalyzed by Candida rugosa lipase (CRL) was found at 29 °C in aqueous and S-ketoprofen was obtained predominantly at 40 °C. However, CRL showed R ... | 2011 | 22113016 |
| Selection of orlistat as a potential inhibitor for lipase from Candida species. | Infections caused by Candida species manifest in a number of diseases, including candidemia, vulvovaginal candidiasis, endocarditis, and peritonitis. Candida species have been reported to possess lipolytic activity due to the secretion of lipolytic enzymes such as esterases, lipases and phospholipases. Extra-cellular hydrolytic enzymes seem to play an important role in Candida overgrowth. Candidiasis is commonly treated with antimycotics such as clotrimazole and nystatin. The antimycotics bind t ... | 2011 | 22125382 |
| fungal peritonitis in peritoneal dialysis: an analysis of 21 cases. | fungal peritonitis is a rare but serious complication in patients undergoing peritoneal dialysis (pd). we aimed to analyze the predisposing factors, clinical aspects, etiological agents, and treatment of fungal peritonitis in patients with pd in our hospital. | 2011 | 20526810 |
| biodiesel production by a mixture of candida rugosa and rhizopus oryzae lipases using a supercritical carbon dioxide process. | in this study, various factors, such as temperature, pressure, agitation speed, water content, and the concentration and ratio of immobilized rol and crl were investigated for the efficient enzymatic production of biodiesel using a supercritical carbon dioxide process. furthermore, a stepwise reaction method for the maintenance of immobilized lipase activity was optimized. optimal conditions for biodiesel production were determined to be as follows: 130 bar pressure, 45 °c temperature, 250 rpm a ... | 2011 | 20813518 |
| immobilization of candida rugosa lipase on glass beads for enantioselective hydrolysis of racemic naproxen methyl ester. | candida rugosa lipase (crl) was immobilized on glutaraldehyde-activated aminopropyl glass beads by using covalent binding method or sol-gel encapsulation procedure and improved considerably by fluoride-catalyzed hydrolysis of mixtures of rsi(och3)3 and si(och3)4. the catalytic properties of the immobilized lipases were evaluated into model reactions, i.e. the hydrolysis of p-nitrophenylpalmitate (p-npp). it has been observed that the percent activity yield of the encapsulated lipase was 166.9, w ... | 2011 | 20846857 |
| improvement of catalytic properties of candida rugosa lipase by sol-gel encapsulation in the presence of magnetic calix[4]arene nanoparticles. | candida rugosa lipase (crl) was encapsulated within a chemically inert sol-gel support prepared by polycondensation with tetraethoxysilane (teos) and octyltriethoxysilane (otes) in the presence of n-methylglucamine based calix[4]arene magnetic nanoparticles. the results indicate that the magnetic calix[4]arene based encapsulated lipase particularly has shown high conversion and enantioselectivity. it has also been noticed that the magnetic calix[4]arene based encapsulated lipase has excellent en ... | 2011 | 21509361 |
| selective biocatalytic aminolysis of (±)-epichlorohydrin: synthesis and icam-1 inhibitory activity of (s)-(+)-3-arylamino-1-chloropropan-2-ols. | regio- and enantioselective synthesis of (s)-(+)-3-arylamino-1-chloropropan-2-ols has been achieved by the epoxide ring opening of (±)-epichlorohydrin with different aromatic amines in the presence of candida rugosa lipase. activities of seven model (s)-(+)-3-arylamino-1-chloropropan-2-ols, out of 10 compounds synthesized, have been evaluated for the inhibition of tumor necrosis factor-a tnf-a) induced expression of intercellular adhesion molecule-1 (icam-1), which is one of the factors responsi ... | 2011 | 21419631 |
| cationic polyelectrolytes-lipases complexes formation as tool for recovery of these enzymes from their natural sources. | in the present paper the formation of complexes between positively charge polyelectrolyte (polyethyleneimine and chitosan) and candida rugosa lipase from a crude extract and porcine lipase from pancreas commercial homogenate preparations were analyzed. the solubility of lipases-cationic polyelectrolytes formation was dependent on: polyelectrolyte densities electrical charge, polyelectrolyte and enzyme concentrations and salts present in the solution. the lipase was recovered from the non-soluble ... | 2011 | 21640134 |
| peritonitis due to candida rugosa: the first case report. | 2010 | 20829554 | |
| site-specific saturation mutagenesis on residues 132 and 450 of candida rugosa lip2 enhances catalytic efficiency and alters substrate specificity in various chain lengths of triglycerides and esters. | the catalytic versatility of recombinant candida rugosa lip2 has been known to have potential applications in industry. in this study, site-specific saturation mutagenesis on residues l132 and g450 of recombinant lip2 has been employed to investigate the impact of both residues on substrate specificity of lip2. point mutations on l132 and g450 were done separately using mutagenic degenerate primer sets containing 32 codons to generate two libraries of mutants in pichia pastoris . replacements of ... | 2010 | 20873770 |
| candida rugosa lipase lip1-polyethyleneglycol interaction and the relation with its partition in aqueous two-phase systems. | the interaction between a lipase from candida rugosa (lip1) and polyethyleneglycols of different molecular masses was studied using fluorescence and circular dichroism approaches in order to be applied to the analysis of the enzyme partition mechanism in aqueous two-phase systems of polyethyleneglycol-potassium phosphate. the decrease of the partition coefficients with the polyethyleneglycol molecular mass showed that the enzyme partition is driven by the excluded volume effect and not by the en ... | 2010 | 19846284 |
| stabilization of candida rugosa lipase during transacetylation with vinyl acetate. | an optimally prepared candida rugosa lipase aggregate cross-linked with bovine serum albumin, was found to overcome acetaldehyde deactivation during transacetylation of a series of benzyl alcohols with vinyl acetate. the formulation, under the same reaction conditions, exhibited 4-30x enhancement in the reaction rate as compared to the celite immobilized lyophilized formulation and 25-133x enhancement as compared to the free lyophilized enzyme depending upon the alcohol chosen. the racemic 1-phe ... | 2010 | 19854046 |
| cloning of a novel lipase gene, lipj08, from candida rugosa and expression in pichia pastoris by codon optimization. | a novel lipase gene, lipj08, was cloned from candida rugosa atcc14830, along with the already reported five lipase genes (lip1-lip5). nucleotide sequencing indicated that the lipj08 gene contains a 1650 bp open reading frame (orf) without introns. the deduced amino acid sequence corresponds to 534 amino acid residues, including a putative signal sequence of 15 amino acid residues. seventeen of the non-universal serine codons (ctg) of lipj08 were converted into universal serine codons (tct) by pc ... | 2010 | 19841868 |
| cloning of the zygosaccharomyces bailii gas1 homologue and effect of cell wall engineering on protein secretory phenotype. | zygosaccharomyces bailii is a diploid budding yeast still poorly characterized, but widely recognised as tolerant to several stresses, most of which related to industrial processes of production. because of that, it would be very interesting to develop its ability as a cell factory. gas1p is a beta-1,3-glucanosyltransglycosylase which plays an important role in cell wall construction and in determining its permeability. cell wall defective mutants of saccharomyces cerevisiae and pichia pastoris, ... | 2010 | 20102600 |
| effect of pore diameter and cross-linking method on the immobilization efficiency of candida rugosa lipase in sba-15. | the influences of pore diameter and cross-linking method on the immobilization efficiency of candida rugosa lipase (crl) in a mesoporous material have been investigated. five kinds of sba-15 with different pore-sizes (6.8nm, 9.1nm, 13.2nm, 15.6nm and 22.4nm) were chosen as the carrier. the one with pore diameter of 15.6nm was proved to be a more suitable immobilization support than the others, and the loading amount reached 343.6mg/g. to solve the leaching problem of the adsorbed enzyme, chitosa ... | 2010 | 20116998 |
| a highly stable yarrowia lipolytica lipase formulation for the treatment of pancreatic exocrine insufficiency. | yarrowia lipolytica lipase has been assumed to be a good candidate for the treatment of fat malabsorption in patients with pancreatic insufficiency. nevertheless, no systematic studies on its stability under physiological conditions pertaining to the human gi (gastrointestinal) tract have been published. stability of various y. lipolytica lipase powder formulations at various physiological ph values as well as the effect of digestive proteases and bile salts on enzyme activity were investigated. ... | 2010 | 20958263 |
| hydrogels of n-isopropylacrylamide copolymers with controlled release of a model protein. | temperature- and ph-sensitive hydrogels, based on n-isopropylacrylamide (nipaam) and itaconic acid (ia), were synthesized by free radical crosslinking copolymerization in the presence of lipase from candida rugosa. the samples were characterized for their sensitivity to the changes of external conditions and the ability to control the release of a hydrophilic model protein, lipase. these hydrogels were highly responsive to temperature and ph, at constant ionic strength. parameters, such as the c ... | 2010 | 19747965 |
| surfactant enhanced ricinoleic acid production using candida rugosa lipase. | in this study, ricinoleic acid was produced on surfactant enhanced castor oil hydrolysis using candida rugosa lipase. the most effective surfactant was span 80. employing fractional factorial design, the most suitable temperature and surfactant concentration were found to be 31 degrees c and 0.257% (w/w in buffer) respectively whereas ph, enzyme concentration, buffer concentration and agitation were identified as the most significant independent variables. a 2(4) full factorial central composite ... | 2010 | 19717301 |
| enzyme-functionalized polymer brush films on the inner wall of silicon-glass microreactors with tunable biocatalytic activity. | the lipase from candida rugosa was immobilized to a poly(methacrylic acid) polymer brush layer, grown on the inner wall of silicon-glass microreactors. the hydrolysis of 4-nitrophenyl acetate was used as a model reaction to study the activity of this biocatalytic system. the amount of bound lipase could be tuned by changing the polymerization time of the brush formation. the michaelis-menten constants and v(max) values, determined for immobilized and free lipase, are similar, demonstrating that ... | 2010 | 20941436 |
| reversible immobilization of candida rugosa lipase on fibrous polymer grafted and sulfonated p(hema/egdma) beads. | poly(2-hydroxyethyl methacrylate/ethylenglycol dimethacrylate) beads were grafted with poly(glycidylmethacrylate) via surface initiated atom transfer radical polymerization. epoxy groups of the grafted polymer were modified in to sulfone groups. sulfonated beads were characterized by swelling studies, ft-ir, sem and elemental analysis, and were used for reversible immobilization of lipase. under given experimental conditions, the beads had an adsorption capacity of 44.7 mg protein/g beads. the a ... | 2010 | 19350276 |
| influence of alcohol treatments on the activity of lipases immobilized on methyl-modified silica aerogels. | the effects of alcohol treatment on the activity and loading amount of candida rugosa lipase (crl), candida antarctica lipase b (calb) and porcine pancreas lipase (ppl) immobilized on methyl-modified silica aerogels were investigated, and the fluorescent analysis was used to explore the change of lipase hydrophobicity in aqueous solution caused by alcohols. it is found that alcohol types and the stages at which alcohol was added significantly influenced the performance of immobilized lipases thr ... | 2010 | 20576560 |
| determination of total glutathione in yeasts by high-performance liquid chromatography with dansylation. | a method to determine the content of total glutathione (gsht) was introduced based on high-performance liquid chromatography (hplc) with dansylation. the minimum detection concentration of gsht was 0.5 microg/ml and the measurable range 1.0-300 microg/ml. gsht in yeasts was obtained by hot-water extraction, gsh complete autoxidation to oxidized glutathione (gssg) in alkaline solution and purification by thin-layer chromatography (tlc). the quantitative determination of gssg was derived by dansyl ... | 2010 | 20653242 |
| [candida rugosa fungemia: new emergent infection case]. | 2010 | 20140322 | |
| miniemulsion as efficient system for enzymatic synthesis of acid alkyl esters. | the aim of this work is to devise an efficient enzymatic process for the production of linear alkyl esters in aqueous miniemulsion systems. the esterification reactions of linear alcohols and carboxylic acids were performed with three different enzymes, commercial amano lipase ps from pseudomonas cepacia, lipase type vii from candida rugosa, and lyophilized fusarium solani pisi cutinase expressed in saccharomyces cerevisiae su50. the miniemulsion system shows a high potential for the synthesis o ... | 2010 | 20503297 |
| immobilization of cross-linked lipase aggregates onto magnetic beads for enzymatic degradation of polycaprolactone. | candida rugosa lipase was immobilized on amino-functionalized magnetic supports via cross-linked enzyme aggregates (clea) and used to enhance the enzymatic degradation of polycaprolactone (pcl). the maximum amounts of lipase immobilized on the magnetic beads using glutaraldehyde as a coupling agent were determined to be 33.7 mg/g of beads with an 81% recovery of activity after immobilization. compared to the free enzyme, the immobilized lipase showed the optimum ph at 1 unit higher (ph 8.0) and ... | 2010 | 20473952 |
| solvent-induced lid opening in lipases: a molecular dynamics study. | in most lipases, a mobile lid covers the substrate binding site. in this closed structure, the lipase is assumed to be inactive. upon activation of the lipase by contact with a hydrophobic solvent or at a hydrophobic interface, the lid opens. in its open structure, the substrate binding site is accessible and the lipase is active. the molecular mechanism of this interfacial activation was studied for three lipases (from candida rugosa, rhizomucor miehei, and thermomyces lanuginosa) by multiple m ... | 2010 | 20812327 |
| biochemical properties of free and immobilized candida rugosa lipase onto al(2)o(3): a comparative study. | abstract: lipase from candida rugosa (crl) was immobilized by physical adsorption onto al(2)o(3). the immobilization yield was more than 95% during 30 min. the properties of free and immobilized enzyme were also searched and compared. the optimum ph was shifted from 7 to 8.5 by immobilization of the enzyme. the maximum activity of the free and the immobilized enzymes occured at 35 and 40°c, respectively. | 2010 | 21117873 |
| in vitro activity of fluconazole and voriconazole against clinical isolates of candida spp. by e-test method. | the in vitro susceptibility of clinical candida isolates towards fluconazole and voriconazole was determined using the e-test method. a total of 41 clinical isolates recovered from patients since 2004 until 2009 from two local hospitals in kuala lumpur, malaysia were used. these comprised candida tropicalis, candida albicans, candida krusei, candida parapsilosis, candida rugosa, candida dubliniensis and candida glabrata. strains from american type culture collection were used as quality control. ... | 2010 | 20962716 |
| enzymatic hydrolysis of polylactic acid fiber. | this study investigated the optimization of the enzymatic processing conditions for polylactic acid (pla) fibers using enzymes consisting of lipases originating from different sources. the hydrolytic activity was evaluated taking into consideration the ph, temperature, enzyme concentration, and treatment time. the structural change of the pla fibers was measured in the optimal treatment conditions. pla fiber hydrolysis by lipases was maximized for lipase from aspergillus niger at 40 °c for 60 mi ... | 2010 | 21038086 |
| recombinant candida rugosa lipase 2 from pichia pastoris: immobilization and use as biocatalyst in a stereoselective reaction. | the characterization of the recombinant candida rugosa lip2 (r-lip2) isoenzyme obtained from fed-batch cultures of pichia pastoris under paox promoter was carried out, determining the optimal ph and temperature as well as their catalytic performance in both hydrolysis and synthesis reactions comparing with purified native lip2 (n-lip2) previously determined. the substrate specificity of r-lip2 in hydrolysis reactions was determined with a series of triacylglycerols and p-nitrophenyl esters of va ... | 2010 | 20945483 |
| development of batch and continuous processes on biodiesel production in a packed-bed reactor by a mixture of immobilized candida rugosa and rhizopus oryzae lipases. | in this study, transesterification and esterification were investigated in batch and continuous process using immobilized candida rugosa and rhizopus oryzae lipases. in the case of batch process, stepwise reaction method was investigated to prevent the lipase deactivation. reaction conditions were as follows: temperature, 45 degrees c; agitation speed, 250 rpm; enzyme concentration, 20%; and water contents 10%. and then, conversion yield was 98.33% at 4 h. in the case of continuous process, circ ... | 2010 | 19898962 |
| dual response surface-optimized synthesis of l-menthyl conjugated linoleate in solvent-free system by candida rugosa lipase. | lipase-catalyzed synthesis of l-menthyl conjugated linoleate in solvent-free system was studied in this paper. duel response surface methodology was employed to explore the factors which would influence the reaction conversion by a range of independent experiments. the conditions of reaction temperature, reaction time, enzyme amount, substrate molar ratio and water content were symmetrically investigated. when the substrates were 1 mmol cla and 1 mmol l-menthol, the maximum conversion (79.1+/-0. ... | 2010 | 19833506 |
| structural classification by the lipase engineering database: a case study of candida antarctica lipase a. | the lipase engineering database (led) integrates information on sequence, structure and function of lipases, esterases and related proteins with the alpha/beta hydrolase fold. a new superfamily for candida antarctica lipase a (cala) was introduced including the recently published crystal structure of cala. since cala has a highly divergent sequence in comparison to other alpha/beta hydrolases, the lipase engineering database was used to classify cala in the frame of the already established class ... | 2010 | 20170513 |
| epidemiology of candidaemia in critically ill trauma patients: experiences of a level i trauma centre in north india. | there is a need to understand the epidemiology and risk factors associated with candidaemia in critically ill trauma patients. the rise in incidence of non-albicans candidaemia and the emergence of antifungal resistance have made such a study necessary. a prospective laboratory-based surveillance study was performed over a period of 21 months (april 2008-december 2009) at a level i trauma centre in new delhi, india. all blood culture samples positive for candida were processed for microbial iden ... | 2010 | 21127153 |
| effect of oxidation level of n(+)-type mesoporous silicon surface on the adsorption and the catalytic activity of candida rugosa lipase. | in this work, we present the synthesis and characterization of n(+)-type porous silicon (psi) layers. our final aim is the fabrication of a biosensor that exploits the semiconductive properties of this material. psi wafers were used as a matrix for enzyme adsorption. these wafers, as a result of their porous nanostructure, had a high surface area (360m(2)/g) and pore size in the range 5-20nm. the freshly prepared psi was stabilized through controlled anodic oxidation. two classes of samples diff ... | 2010 | 20188377 |
| lipase-catalyzed production of a bioactive terpene ester in supercritical carbon dioxide. | direct esterification of alpha-terpineol and acetic anhydride catalyzed by candida rugosa lipase was performed in supercritical carbon dioxide (sc-co(2)) with organic solvent serving as co-solvents. the highest yield of terpinyl acetate after 1.5h of reaction performance (95.1%) was obtained in sc-co(2) with n-heptane serving as a co-solvent and immobilized candida rugosa lipase as an enzyme at 50 degrees c. the optimal pressure for terpinyl acetate synthesis in sc-co(2) medium was 10 mpa. aceti ... | 2010 | 20219605 |
| candida rugosa: a possible emerging cause of candidaemia in trauma patients. | candida rugosa appears to be emerging as a distinctive cause of candidaemia in recent years. candidaemia due to this species is important to recognise because of its decreased susceptibility to azoles. | 2010 | 20658166 |
| determination of microbial diversity in meju, fermented cooked soya beans, using nested pcr-denaturing gradient gel electrophoresis. | to identify the microbiota in meju, fermented cooked soya beans, that may directly affect the microbial communities of korean fermented soya bean foods. | 2010 | 20704651 |
| screening of food grade lipases to be used in esterification and interesterification reactions of industrial interest. | seven food grade commercially available lipases were immobilized by covalent binding on polysiloxane-polyvinyl alcohol (pos-pva) hybrid composite and screened to mediate reactions of industrial interest. the synthesis of butyl butyrate and the interesterification of tripalmitin with triolein were chosen as model reactions. the highest esterification activity (240.63 microm/g min) was achieved by candida rugosa lipase, while the highest interesterification yield (31%, in 72 h) was achieved by lip ... | 2010 | 19263247 |
| effect of a buffer mixture system on the activity of lipases during immobilization process. | in this study, the effects of various buffers and ionic strengths on the immobilization of candida rugosa and rhizopus oryzae lipases were investigated to enhance the activities of the immobilized lipases. among the various buffers, the optimal buffers and ionic strength for the immobilization of c. rugosa and r. oryzae lipases were determined to be a mixture of 0.25m mops and sodium phosphate buffer (ph 6.5). moreover, the activities of immobilized c. rugosa and r. oryzae lipases under their op ... | 2010 | 19361984 |
| identification of candida species isolated from bovine mastitic milk and their in vitro hemolytic activity in western turkey. | in this study, identification of 207 candida isolates, previously isolated from mastitic bovine quarter milk samples at the level of genus, was made using api 20 c aux system. the most frequently isolated species were candida krusei (34.8%), followed by candida rugosa (16.4%), candida kefyr (12.6%), candida albicans (10.1%), and candida tropicalis (9.2%). less common isolates were candida zeylanoides (5.8%), candida parapsilosis (4.3%), candida guilliermondii (3.4%), candida famata (1.9%), and c ... | 2010 | 19921462 |
| rigorous kinetic model considering positional specificity of lipase for enzymatic stepwise hydrolysis of triolein in biphasic oil-water system. | a rigorous kinetic model describing the stepwise triglyceride hydrolysis at the oil-water interface, based on the ping pong bi bi mechanism using suspended lipase having positional specificity, was constructed. the preference of the enzyme to cleave to the ester bonds at the edge and the center of the glycerol backbone of the substrates (tri-, di- or monoglyceride) was incorporated in the model. this model was applied to the experimental results for triolein hydrolysis using suspended porcine pa ... | 2010 | 20024663 |
| synthesis of terpinyl acetate by lipase-catalyzed esterification in supercritical carbon dioxide. | terpinyl acetate was successfully synthesized from alpha-terpineol and acetic anhydride in supercritical carbon dioxide (sc-co(2)) by enzymatic catalysis. five different lipases (candida rugosa type vii, amano ps, amano ap-6, amano g and lipozyme rm im) as biocatalysts for the terpinyl acetate synthesis were compared. an esterification extent of 53.0% was obtained in continuous operation using acetic anhydride as acyl donor and c. rugosa lipase as enzyme at 10mpa and 50 degrees c for 1.5h. tempe ... | 2010 | 20096575 |
| multidrug resistant yeasts in synanthropic wild birds. | the aim of this study was to investigate the presence of multidrug resistant yeasts in the faeces of synanthropic wild birds from the bangsar suburb of kuala lumpur. | 2010 | 20307325 |
| [yeasts in domestic animals: species identification and susceptibility to antifungals]. | yeasts frequently colonize various kinds of domestic animals, but may also cause serious diseases. the aim of this study was to identify yeast isolates collected from dogs, cows and pigs, and to determine their in vitro antifungal susceptibility. fifty-six yeast isolates from dogs (n = 24), cows (n = 20), and pigs (n = 12) were investigated. appearance of colonies grown on sabouraud agar, micromorphology on rice agar, as well as assimilation and fermentation of various carbon and nitrogen source ... | 2010 | 20401831 |
| staphylococcal lipases stereoselectively hydrolyse the sn-2 position of monomolecular films of diglyceride analogs. application to sn-2 hydrolysis of triolein. | using the monomolecular film technique, a kinetic study on the stereoselectivity of nine staphylococcal lipase forms was carried out with three pairs of enantiomers from diglyceride analogs (didecanoyl-deoxyamino-o-methyl glycerol, ddg) containing a single hydrolysable decanoyl ester group and two lipase-resistant groups. our results show that the kinetic profiles of the wild type, the recombinant untagged and the recombinant tagged forms of staphylococcal lipases are significantly different. as ... | 2010 | 20403605 |
| study of a sterol esterase secreted by ophiostoma piceae: sequence, model and biochemical properties. | an extracellular sterol esterase from ophiostoma piceae efficiently hydrolyzes sterol esters, triglycerides and p-nitrophenol esters. cdna was screened with a probe obtained by pcr using as primers oligonucleotides corresponding to the n-terminal and internal mature enzyme sequences and complete sequence was obtained by 3' rapid amplification of cdna end (race) and inverse pcr. the o. piceae esterase gene had a length of 1.8 kbp and lacked introns. a search for proteins with related amino acid s ... | 2009 | 19281875 |
| maximization of bioconversion of castor oil into ricinoleic acid by response surface methodology. | in this study, response surface methodology was applied to optimize process variables like temperature, ph, enzyme concentration (mg/g oil), and buffer concentration (g/g oil) for hydrolysis of castor oil using candida rugosa lipase. a 2(4) full factorial central composite design was used to develop the quadratic model that was subsequently optimized and the optimal conditions were as follows: temperature 40 degrees c, ph 7.72, enzyme concentration 5.28 mg/g oil, buffer concentration 1g/g oil an ... | 2009 | 19419859 |
| candida rugosa: a distinctive emerging cause of candidaemia. a case report and review of the literature. | candida rugosa is a rare cause of candidaemia, but important to recognize because of frequent azole-resistance and its association with catheters and total parenteral nutrition. recommended therapy is an echinocandin or amphotericin, and catheter discontinuation. fluconazole might be substituted based on susceptibility testing and a clinical response to initial therapy. | 2009 | 19922077 |
| effects of alcohol and buffer treatments on the activity and enantioselectivity of candida rugosa lipase. | several treatments were employed on candida rugosa lipase (crl) to improve its biocatalytic performance. besides conventional alcohol treatment conditions, the effects of ph of the buffer solution used in the treatment as well as the changes in stirring, dialysis, and centrifugation steps of the treatment procedure were investigated for the first time for the resolution of racemic naproxen methyl ester. the highest enantioselectivity and conversion in s-naproxen production were achieved by crl t ... | 2009 | 19291575 |
| production of ricinoleic acid from castor oil by immobilised lipases. | porcine pancreatic lipase (ppl), candida rugosa lipase (crl), and castor bean lipase (cbl) were immobilized on celite by deposition from aqueous solution by the addition of hexane. lipolytic performance of free and immobilized lipases were compared and optimizations of lipolytic enzymatic reactions conditions were performed by free and immobilized derivatives using olive oil as substrate. afterwards, the influence on lipolysis of castor oil of free lipases and immobilized lipase derivatives have ... | 2009 | 19291579 |
| media formulation using complex organic nutrients for improved activity, productivity, and yield of candida rugosa lipase and esterase enzymes. | candida rugosa is an excellent source of multiple lipase and esterase enzymes; therefore, it is of technological importance to formulate the medium that provides high activity for each enzyme. in this work, the cultivation medium comprising complex nutrients that provided the highest activity, productivity, and yield of c. rugosa enzymes individually was formulated. time courses of the extracellular and intracellular lipase and esterase activities of c. rugosa were represented and the role of pr ... | 2009 | 19431047 |
| evaluation of chrom-pal medium for the isolation and direct identification of candida dubliniensis in primary cultures from the oral cavity. | candida albicans is the species most frequently isolated from oral specimens, but the recovery of other candida species such as candida dubliniensis is increasing. differentiation of c. dubliniensis from c. albicans requires special tests and both species are misidentified in some studies. chrom-pal (ch-p) is a novel chromogenic medium used in our laboratory for differentiation between c. albicans and c. dubliniensis on the basis of colony colour and morphology, and chlamydospore production. the ... | 2009 | 19608690 |
| pretreatment of candida rugosa lipase with soybean oil before immobilization on beta-cyclodextrin-based polymer. | in this study, candida rugosa lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. the pretreated c. rugosa lipase was immobilized on beta-cyclodextrin-based polymer by crosslinking with hexamethylene diisocyanate (hmdi). this pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. the pretrea ... | 2009 | 19091527 |
| preparation and characterization of chitosan membranes by using a combined freeze gelation and mild crosslinking method. | when gelification is performed by freezing-thawing repeated cycles, the resultant gel-like polymer systems are called cryogels. this work aims to assess the effect of the addition of glutaraldehyde and 18 crown ether-6 on surface properties and protein loading of dried chitosan cryogel films. residual water content of treated chitosan membranes ranged between 11.93 and 13.86%, while their water activities vary from 0.5 to 0.7 (measured from 4 to 60 degrees c). based on thermal data, water evapor ... | 2009 | 18546023 |
| biological treatment of olive mill wastewater by non-conventional yeasts. | the ability of lipolytic yeasts to grow on olive mill wastewater (omw)-based medium and to produce high-value compounds while degrading this waste, was tested. omw collected from three-phase olive mills from the north region of portugal were characterized and used. omw with cod ranging from 100 g l(-1) to 200 g l(-1) were supplemented with yeast extract and ammonium chloride. studies of omw consumption were carried out in batch cultures of candida rugosa, candida cylindracea and yarrowia lipolyt ... | 2009 | 19231162 |
| lipase nanogel catalyzed transesterification in anhydrous dimethyl sulfoxide. | the present work showed that candida rugosa lipase, which is inactive in anhydrous dimethyl sulfoxide (dmso), has been granted its original catalytic activity and greatly enhanced stability when encapsulated into a polyacrylamide nanogel. the molecular simulation and structural analysis suggested that the polyacrylamide nanogel shielded the extraction of essential water and maintained the native configuration of encapsulated lipase in anhydrous dmso at an elevated temperature. the electron and f ... | 2009 | 19361213 |
| impact of inoculation strategy on the progress of candida rugosa cultivation. | batch cultivation of candida rugosa for the production of lipase and esterase enzymes was carried out by employing two different inoculation strategies. the effects of triolein (2, 3 and 5 g/l) and oleic acid (3 g/l) as carbon sources of the enzyme production medium on the activity, productivity, and yield of enzymes were also compared for both strategies. inoculation of the cells into the enzyme production medium either directly or after cultivation in a pre-culture medium rich in glucose affec ... | 2009 | 19412822 |
| production of 3-hydroxypropionic acid from acrylic acid by newly isolated rhodococcus erythropolis lg12. | a novel microorganism, designated as lg12, was isolated from soil based on its ability to use acrylic acid as the sole carbon source. an electron microscopic analysis of its morphological characteristics and phylogenetic classification by 16s rrna homology showed that the lg12 strain belongs to rhodococcus erythropolis. r. erythropolis lg12 was able to metabolize a high concentration of acrylic acid (up to 40 g/l). in addition, r. erythropolis lg12 exhibited the highest acrylic acid-degrading ac ... | 2009 | 19494695 |
| modelling substrate specificity and enantioselectivity for lipases and esterases by substrate-imprinted docking. | previously, ways to adapt docking programs that were developed for modelling inhibitor-receptor interaction have been explored. two main issues were discussed. first, when trying to model catalysis a reaction intermediate of the substrate is expected to provide more valid information than the ground state of the substrate. second, the incorporation of protein flexibility is essential for reliable predictions. | 2009 | 19493341 |
| commercially viable resolution of ibuprofen. | ibuprofen belongs to the non-steroidal anti-inflammatory drug (nsaid) family known as profens. studies demonstrate that (s-ibuprofen is 160 times more potent than (r-ibuprofen in vitro, while the accumulation of (r-ibuprofen can cause serious side effects such as gastrointestinal pain. candida rugosa lipase was used to enantioselectively esterify racemic ibuprofen with decan-1-ol and butan-1-ol in cyclohexane with an enantiomeric ratio (e) of 130 and 46, respectively, in up to 46% conversion. se ... | 2009 | 19455529 |
| minor enantiomer recycling: metal catalyst, organocatalyst and biocatalyst working in concert. | a minor enantiomer recycling one-pot procedure employing two reinforcing chiral catalysts has been developed. continuous regeneration of the achiral starting material is effected via selective enzyme-catalyzed hydrolysis of the minor product enantiomer from lewis acid-lewis base catalyzed addition of acyl cyanides to prochiral aldehydes in a two-phase solvent system. the process provides o-acylated cyanohydrins in close to perfect enantioselectivities, higher than those obtained in the direct pr ... | 2009 | 19768712 |
| immobilization of candida rugosa lipase on sporopollenin from lycopodium clavatum. | sporopollenin is a natural polymer obtained from lycopodium clavatum, which is highly stable with constant chemical structure and has high resistant capacity to chemical attack. in this study, immobilization of lipase from candida rugosa (crl) on sporopollenin by adsorption method is reported for the first time. besides this, the enzyme adsorption capacity, activity and thermal stability of immobilized enzyme have also been investigated. it has been observed that under the optimum conditions (sp ... | 2009 | 19583977 |
| understanding structural features of microbial lipases--an overview. | the structural elucidations of microbial lipases have been of prime interest since the 1980s. knowledge of structural features plays an important role in designing and engineering lipases for specific purposes. significant structural data have been presented for few microbial lipases, while, there is still a structure-deficit, that is, most lipase structures are yet to be resolved. a search for 'lipase structure' in the rcsb protein data bank (http://www.rcsb.org/pdb/) returns only 93 hits (as o ... | 2008 | 19609386 |