Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| langmuir-blodgett film of hydrophobin protein from pleurotus ostreatus at the air-water interface. | we present results concerning the formation of langmuir-blodgett (lb) films of a class i hydrophobin from pleurotus ostreatus at the air-water interface, and their structure as langmuir-blodgett (lb) films when deposited on silicon substrates. lb films of the hydrophobin were investigated by atomic force microscopy (afm). we observed that the compressed film at the air-water interface exhibits a molecular depletion even at low surface pressure. in order to estimate the surface molecular concentr ... | 2008 | 18925762 |
| green mold diseases of agaricus and pleurotus spp. are caused by related but phylogenetically different trichoderma species. | abstract producers of champignon (agaricus bisporus) and oyster mushroom (pleurotus ostreatus) are facing recent incidents of green mold epidemics in hungary. we examined 66 trichoderma strains isolated from agaricus compost and pleurotus substrate samples from three hungarian mushroom producing companies by a polymerase chain reaction-based diagnostic test for t. aggressivum, sequence analysis of the internal transcribed spacer region 1 (its1) and its2 and (selectively) of the fourth and fifth ... | 2007 | 18943294 |
| structural characterization and in vitro antitumor activity of a novel polysaccharide isolated from the fruiting bodies of pleurotus ostreatus. | a novel water-soluble polysaccharide (pops-1) was obtained from the fruiting bodies of pleurotus ostreatus by hot water extraction, ethanol precipitation, and fractionated by deae-cellulose ion exchange chromatography and sepharose cl-6b gel filtration chromatography using an atka explore 100 purifier. the structure characterization and antitumor activity of the pops-1 were evaluated in this paper. according to gc analysis, hpgpc, ft-ir, partial acid hydrolysis, periodate oxidation and smith deg ... | 2009 | 18954976 |
| synergic effect studies of the bi-enzymatic system laccase-peroxidase in a voltammetric biosensor for catecholamines. | several bi-enzymatic carbon paste biosensors modified with enzymes laccase from pleurotus ostreatus fungi and peroxidase from zucchini (cucurbita pepo) were constructed for evaluating the synergic effect of the two enzymes on the voltammetric biosensor response for various catecholamines. initially was investigated the effect of ph from 5.0 to 7.5, temperature from 25 to 50 degrees c, initial stirring time from 30 to 150 s, scan rate from 10 to 60 mvs(-1) and potential pulse amplitude from 10 to ... | 2003 | 18968977 |
| myo- and hepatotoxic effects of cultivated mushrooms in mice. | mushrooms are currently examined for their potential as functional foods. at the same time, novel types of mushroom intoxications, such as rhabdomyolysis after prolonged consumption, have been described in edible species. the aim of the present study was to perform an acute toxicity test to establish if the most commonly cultivated species would have myo- or hepatotoxic effects. mice (n=6/group) were exposed to 3, 6 or 9 g agaricus bisporus, lentinus edodes or pleurotus ostreatus kg body mass(-1 ... | 2009 | 18976689 |
| comparison of zns semiconductor nanoparticles capped with various functional groups as the matrix and affinity probes for rapid analysis of cyclodextrins and proteins in surface-assisted laser desorption/ionization time-of-flight mass spectrometry. | zinc sulfide (zns) semiconductor nanoparticles (nps) capped with a variety of functional groups including bare zns nps, 3-mercaptopropanoic acid (zns-3-mpa), sodium citrate (zns-citrate), cysteamine (zns-cys), and 2-mercaptoethane sulfonate (zns-2-mes) have been investigated as the matrix and affinity probes for analysis of alpha-, beta-, and gamma-cyclodextrins (cds), ubiquitin, and insulin in biological samples by using surface-assisted laser desorption/ionization time-of-flight mass spectrome ... | 2008 | 18991387 |
| pleurotus ostreatus inhibits proliferation of human breast and colon cancer cells through p53-dependent as well as p53-independent pathway. | in spite of the global consumption of mushrooms, only two epidemiological studies demonstrated an inverse correlation between mushroom intake and the risk of cancer. therefore, in the present study we evaluated whether extracts from edible mushrooms agaricus bisporus (portabella), flammulina velutipes (enoki), lentinula edodes (shiitake) and pleurotus ostreatus (oyster) affect the growth of breast and colon cancer cells. here, we identified as the most potent, p. ostreatus (oyster mushroom) whic ... | 2008 | 19020765 |
| the pleurotus ostreatus laccase multi-gene family: isolation and heterologous expression of new family members. | this work was aimed at identifying and at characterizing new pleurotus ostreatus laccases, in order to individuate the most suitable biocatalysts for specific applications. the existence of a laccase gene clustering was demonstrated in this basidiomycete fungus, and three new laccase genes were cloned, taking advantage of their closely related spatial organization on the fungus genome. cdnas coding for two of the new laccases were isolated and expressed in the yeasts saccharomyces cerevisiae and ... | 2009 | 19034452 |
| characterization of radical intermediates in laccase-mediator systems. a multifrequency epr, endor and dft/pcm investigation. | suitable low molecular-weight compounds, called mediators, can be used in combination with the phenol-oxidase enzyme laccase to indirectly oxidize large organic substrates, such as environmental pollutants, which are not laccase natural substrates. the oxidation of two different synthetic redox mediators, violuric acid (vio) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (abts) has been studied under catalysis of two laccases from white-rot fungi (trametes versicolor and pleurotus o ... | 2008 | 19060974 |
| effect of various substrates on the growth and quality of mushrooms. | the effect of different biowastes such as paddy straw, sorghum straw, sugarcane molasses, saw dust and paper waste on the growth and biochemical constituents of oyster mushroom (pleurotus florida) was studied. favourable conditions were created to attain the maximum yield of mushrooms. the results reveled that mushroom growth was better in paddy straw followed by sugarcane molasses and least in wood saw dust and paper waste. the growth of mushrooms may be coincided with type of substrates used t ... | 2007 | 19070009 |
| the effect of pleurotus spp. fungi on chemical composition and in vitro digestibility of rice straw. | this study was carried out to test the potentially of using rice straw substrate for the cultivation of four pleurotus species including pleurotus florida, pleurotus djamor, pleurotus sajor-caju and pleurotus ostreatus and the effect of these species on the chemical composition, cell wall degradation and digestibility of rice straw. rice straw soaked in water for 24 h and then it was pasteurized at 100 degrees c for 6 h. rice straw was inoculated with spawns of four pleurotus fungi (pleurotus fl ... | 2007 | 19070114 |
| hybrid production of oyster mushroom pleurotus ostreatus (jacq: fries) kummer. | optimization of industrial mushroom production depends on improving the culture process and breeding new strains with higher yields and productivities. so many works have been done on process improvement, although few systematic studies of genetic breeding of pleurotus ostreatus strains have been reported. the major aim of hybridization is to combine desirable characteristics from different strains and create variability in the existing germ plasm. in this study, we used a breeding approach to h ... | 2007 | 19070153 |
| effect of substrate quantity and shelf position on yield of oyster mushroom (pleurotus sajor caju). | an experiment was conducted in harare (zimbabwe) in 2004 to investigate the effect of substrate (wheat straw) quantity and shelf position on yield of oyster mushroom (pleurotus sajor-caju) using plastic tray culture. the experiment was laid out as a 2-way factorial in a randomized block design with five substrate quantities (2, 4, 6, 8 and 10 kg of wheat straw per tray) and two shelf positions (0.5 and 1.2 m above ground). mushroom yield increased with an increased substrate quantity of up to 6 ... | 2007 | 19090170 |
| telomere organization in the ligninolytic basidiomycete pleurotus ostreatus. | telomeres are structural and functional chromosome regions that are essential for the cell cycle to proceed normally. they are, however, difficult to map genetically and to identify in genome-wide sequence programs because of their structure and repetitive nature. we studied the telomeric and subtelomeric organization in the basidiomycete pleurotus ostreatus using a combination of molecular and bioinformatics tools that permitted us to determine 19 out of the 22 telomeres expected in this fungus ... | 2009 | 19114509 |
| selection of trichoderma strains capable of increasing laccase production by pleurotus ostreatus and agaricus bisporus in dual cultures. | to select trichoderma strains for enhanced laccase production in pleurotus ostreatus or agaricus bisporus cultures. | 2009 | 19120619 |
| an examination of antibacterial and antifungal properties of constituents of shiitake (lentinula edodes) and oyster (pleurotus ostreatus) mushrooms. | antibiotic agents have been in widespread and largely effective therapeutic use since their discovery in the 20th century. however, the emergence of multi-drug resistant pathogens now presents an increasing global challenge to both human and veterinary medicine. it is now widely acknowledged that there is a need to develop novel antimicrobial agents to minimize the threat of further antimicrobial resistance. with this in mind, a study was undertaken to examine the antimicrobial properties of aqu ... | 2009 | 19161947 |
| emulsifying agent production during pahs degradation by the white rot fungus pleurotus ostreatus d1. | for the first time the production of an emulsifying agent during phthalic, 2,2'-diphenic and alpha-hydroxy-beta-naphthoic acids, phenanthrene, anthracene, fluorene, pyrene, fluoranthene, and chrysene degradation by white rot fungus pleurotus ostreatus was found. the emulsifying activity of the cultivation medium after degradation of these compounds was assessed. maximal activities were found in the presence of chrysene (48.4%) and alpha-hydroxy-beta-naphthoic acid (52.2%). emulsifying activity i ... | 2009 | 19194747 |
| biodegradation of endocrine-disrupting compounds and suppression of estrogenic activity by ligninolytic fungi. | endocrine-disrupting compounds (edcs) represent a large group of substances of natural and anthropogenic origin. they are widely distributed in the environment and can pose serious risks to aquatic organisms and to public health. in this study, 4-n-nonylphenol, technical 4-nonylphenol, bisphenol a, 17alpha-ethinylestradiol, and triclosan were biodegraded by eight ligninolytic fungal strains (irpex lacteus 617/93, bjerkandera adusta 606/93, phanerochaete chrysosporium me 446, phanerochaete magnol ... | 2009 | 19243809 |
| pilot study of bioaccumulation and distribution of cesium, potassium, sodium and calcium in king oyster mushroom (pleurotus eryngii) grown under controlled conditions. | this pilot study presents preliminary results on interrelations between alkali and alkaline earth elements during their transfer to mycelium and fruitbodies of saprophytic fungi. the accumulation and distribution of four elements (cesium, potassium, sodium, and calcium) was evaluated in king oyster mushroom (pleurotus eryngii) cultivated under controlled conditions. elemental composition of caps, stipes, and the substrate was analyzed by atomic absorption/emission spectroscopy to evaluate discri ... | 2013 | 19260229 |
| biosorption of cadmium (ii) and lead (ii) from aqueous solutions using mushrooms: a comparative study. | sorption capacity of oyster mushroom (pleurotus platypus), button mushroom (agaricus bisporus) and milky mushroom (calocybe indica) were evaluated on biosorption of heavy metals, viz. cadmium (ii) and lead (ii) from aqueous solutions. the optimum sorption conditions were studied for each metal separately. the desired ph of the aqueous solution was found to be 6.0 for the removal of cadmium (ii) and 5.0 for removal of lead (ii) for all the mushrooms. the percent removal of both the metals was fou ... | 2009 | 19285798 |
| anticataractogenic effect of an extract of the oyster mushroom, pleurotus ostreatus, in an experimental animal model. | to evaluate the efficacy of pleurotus ostreatus extract in preventing selenite-induced cataractogenesis. | 2009 | 19373574 |
| [the composition of volatile components of cepe (boletus edulis) and oyster mushrooms (pleurotus ostreatus)]. | the composition of aroma compounds in cooked and canned cepe (boletus edulis) and in cooked oyster mushrooms (pleurotus ostreatus) is studied using capillary gas chromatography and chromatography-mass spectrometry. it is found that unsaturated alcohols and ketones containing eight atoms of carbon determine the aroma of raw mushrooms and take part in the formation of the aroma of cooked mushrooms as well. the content of these compounds was the highest in canned cepes. in oyster mushrooms, the con ... | 2009 | 19382709 |
| spent mushroom substrates as component of growing media for germination and growth of horticultural plants. | this research work was conducted in order to investigate the possibility of using spent mushroom substrate (sms) in the production of horticultural seedlings replacing part of the peat in the growing media. three vegetable species with different salt sensitivities, the less sensitive being tomato (lycopersicon esculentum var. muchamiel), the moderately salt-sensitive being courgette (cucurbita pepo l. var. afrodite f1) and the most salt-sensitive being pepper (capsicum annum l. var. lamuyo f1) w ... | 2009 | 19409775 |
| intracellular substrates of a heme-containing ascorbate oxidase in pleurotus ostreatus. | a novel heme-containing ascorbate oxidase isolated from oyster mushroom, pleurotus ostreatus, catalyzes oxidation of ascorbic acid (kim et al., 1996). in this report, we describe the identification of intracellular substrates of the enzyme in the mushroom. six compounds, which can serve as substrate of the heme-containing ascorbate oxidase, were identified as l-ascorbic acid, d-erythroascorbic acid, 5-o-(alpha-d-glucopyranosyl)-d-erythroascorbic acid, 5-o-(alpha-d-xylopyranosyl)-d-erythroascorbi ... | 2009 | 19412602 |
| bleach enhancement of mixed wood pulp by xylanase-laccase concoction derived through co-culture strategy. | mixed enzyme preparation having both xylanase and laccase activity was evaluated for its bleach enhancing ability of mixed wood pulp. the enzyme was produced through co-cultivation of mutant penicillium oxalicum sau(e)-3.510 and pleurotus ostreatus mtcc 1804 under solid-state fermentation. bleaching of pulp with mixed enzyme had resulted into a notable decrease in kappa number and increased brightness as compared to xylanase alone. analysis of bleaching conditions had denoted that 8 iu g(-1) of ... | 2010 | 19421722 |
| biodegradation aspects of polycyclic aromatic hydrocarbons (pahs): a review. | pahs are aromatic hydrocarbons with two or more fused benzene rings with natural as well as anthropogenic sources. they are widely distributed environmental contaminants that have detrimental biological effects, toxicity, mutagenecity and carcinogenicity. due to their ubiquitous occurrence, recalcitrance, bioaccumulation potential and carcinogenic activity, the pahs have gathered significant environmental concern. although pah may undergo adsorption, volatilization, photolysis, and chemical degr ... | 2009 | 19442441 |
| [effect of cultivation in zinc media on the growth of and the degree of zinc in organic form in transgenic metallothionein mushroom]. | metallothioneins (mts) are a family of low molecular weight, cysteine-rich and metal-binding functional proteins. transgenic mt mushroom can be used as functional food additives, but its zinc-enriching ability has not been studied systemically until now. the zinc contents in mycelia of transgenic mt mushroom (pleurotus ostreatus) and wild type mushroom mycelia cultivated in different zinc concentration media were analyzed by icp-oes. the growth status, zinc-enriching ability and degree of zinc i ... | 2009 | 19455831 |
| ostreolysin induces sustained contraction of porcine coronary arteries and endothelial dysfunction in middle- and large-sized vessels. | ostreolysin (oly), a cytolytic and cardiotoxic protein from the oyster mushroom (pleurotus ostreatus), is lethal for mice with an ld(50) of 1170 microg/kg following intravenous application. its cardiotoxicity is associated with hyperkalemia, which is probably a consequence of potassium released from the lysed cells. moreover, sub-micromolar concentrations of oly induce a concentration-dependent increase in rat aortic ring tension, suggesting that ischaemia, and consequent hypoxic injury of cardi ... | 2009 | 19524606 |
| laccase-membrane reactors for decolorization of an acid azo dye in aqueous phase: process optimization. | in the present investigation, performance of various laccase-membrane reactor configurations including direct enzyme contact, enzyme impregnated, immobilized enzyme and a reactor system based on laccase immobilization in chitosan membranes for decolorization of azo dye (acid black 10 bx) were examined using laccase enzyme purified from white rot fungi pleurotus ostreatus 1804. a five-step laccase purification procedure was employed, which improved the enzymatic activity by 8.27 folds. laccase wa ... | 2009 | 19540548 |
| optimized protocol for the 2-de of extracellular proteins from higher basidiomycetes inhabiting lignocellulose. | basidiomycetes inhabiting lignocellulose comprise an important class of filamentous fungi with ecological and biotechnological relevance. extracellular enzymes of wood-degrading fungi such as laccases, manganese-dependent (or independent) peroxidases, cellulases and xylanases are of considerable interest for biotechnological applications. still, proteomic studies of fungal secretomes based on 2-de can be very challenging due to low protein concentrations and variable amounts of fungal metabolite ... | 2009 | 19593751 |
| a glycoprotein extracted from golden oyster mushroom pleurotus citrinopileatus exhibiting growth inhibitory effect against u937 leukemia cells. | mushrooms have become popular sources of natural antitumor, antiviral, antibacterial, antioxidative, and immunomodulatory agents. golden oyster mushroom, pleurotus citrinopileatus , is a common mushroom in oriental countries for human consumption. we isolated a functional protein (pcp-3a) from the fresh fruiting body of this mushroom. the isolation procedure included ammonium sulfate fractionation, deae-sepharose cl-6b ion exchange chromatography, and sephacryl s-300 gel filtration. electrophore ... | 2009 | 19606865 |
| are stag beetles fungivorous? | stag beetle larvae generally feed on decaying wood; however, it was unknown whether they can use wood-rotting fungi alone as food. here, to clarify this, newly hatched larvae of dorcus rectus (motschulsky) (coleoptera: lucanidae) were reared for 14 days on artificial diets containing a fixed amount of freeze-dried mycelia of the following fungi: bjerkandera adusta, trametes versicolor, pleurotus ostreatus, and fomitopsis pinicola. the mean incremental gain in larval body mass was greatest on die ... | 2009 | 19607834 |
| lignocellulolytic enzyme activity, substrate utilization, and mushroom yield by pleurotus ostreatus cultivated on substrate containing anaerobic digester solids. | solid waste from anaerobic digestion of litter from the commercial production of broiler chickens has limited use as fertilizer. its disposal is a major problem for digester operators who are seeking alternative use for anaerobic digester solids, also referred to as solid waste (sw). the use of sw as substrates for the cultivation of pleurotus ostreatus strain mbfbl400 was investigated. lignocellulolytic enzymes activity, substrate utilization, and mushroom yield were evaluated in ten different ... | 2009 | 19618225 |
| molecular characterization of the phenol oxidase (pox2) gene from the ligninolytic fungus pleurotus ostreatus. | the gene (pox2) encoding a phenol oxidase from pleurotus ostreatus, a lignin-degrading basidiomycete, was sequenced and the corresponding pox2-cdna was also synthesized, cloned and sequenced. the isolated gene consisted of 2674 bp, with the coding sequence interrupted by 19 introns and flanked by an upstream region in which the putative metal-responsive elements (mres) were determined in the promoter region (849 bp), where mre 1, 2, 3 and 4 were located in positions -20, -60, -236 and -297. a fu ... | 2009 | 19622072 |
| reproducible and controllable light induction of in vitro fruiting of the white-rot basidiomycete pleurotus ostreatus. | fruiting is a crucial developmental process in basidiomycetes yet the genetic and molecular factors that control it are not yet fully understood. the search for fruiting inducers is of major relevance for both basic research and for their use in industrial applications. in this paper, an efficient and reproducible protocol for controlled fruiting induction of pleurotus ostreatus growing on synthetic medium is described. the protocol is based on the control of light intensity and photoperiod and ... | 2009 | 19640403 |
| study of new ways of supplementary and combinatory therapy of rheumatoid arthritis with immunomodulators. glucomannan and imunoglukán in adjuvant arthritis. | we studied the anti-arthritic activity of glucomannan (gm) isolated from candida utilis and of imunoglukán, a beta-(1,3/1,6)-d-glucan (img) isolated from pleurotus ostreatus. adjuvant arthritis (aa) was induced intradermally by the injection of mycobacterium butyricum in incomplete freund's adjuvant to lewis rats. blood for biochemical and immunological analysis was collected on experimental days 1, 14, 21, and 28. a clinical parameter--hind paw volume (hpv)--was also measured. the detection of ... | 2009 | 19651805 |
| degradation of aflatoxin b(1) by fungal laccase enzymes. | the enzymatic degradation of aflatoxin b(1) (afb(1)) by white rot fungi through laccase production was investigated in different liquid media. a significant (p<0.0001) correlation was observed between laccase activity and afb(1) degradation exhibited by representatives of peniophora and pleurotus ostreatus cultivated in minimal salts (msm) (r=0.93) and mineral salts - malt extract (msb-meb) (r=0.77) liquid media. peniophora sp. scc0152 cultured in msb-meb liquid medium supplemented with veratryl ... | 2009 | 19683355 |
| the potential of pleurotus-treated olive mill solid waste as cattle feed. | the aims of the current study were to follow: (1) the capability of the edible mushroom pleurotus ostreatus to degrade cell wall components and soluble phenols of the olive mill solid waste (omsw), and improve it for ruminant nutrition (2) the fate of oil and the lipid-soluble compounds tocopherols, squalene and beta-sitosterol in the fermented omsw. a significant decrease in oil and lipid-soluble compounds with a concomitant shift in the fatty acid profile and degradation of soluble phenols too ... | 2009 | 19683915 |
| production of laccases by pleurotus ostreatus in submerged fermentation in co-culture with trichoderma viride. | to evaluate the production and stability of laccases by pleurotus ostreatus in liquid co-cultures with trichoderma viride as a function of infection time and agitation rate. | 2010 | 19709340 |
| prevalence of ewingella americana in retail fresh cultivated mushrooms (agaricus bisporus, lentinula edodes and pleurotus ostreatus) in zaragoza (spain). | the prevalence and mycopathogenic potential of enterobacteriaceae (especially ewingella americana) in cultivated mushrooms were studied. a total of 95 samples of agaricus bisporus, lentinula edodes and pleurotus ostreatus were analyzed to quantify the enterobacteriaceae and to identify the species isolated. the host pathogenicity test was used to verify their mycopathogenic potential. the genus pseudomonas was also quantified, since it is the predominant bacterial group in cultivated mushrooms. ... | 2004 | 19712317 |
| increase of laccase activity during interspecific interactions of white-rot fungi. | white-rot fungi are of interest due to their ability to degrade lignin. lignin-degrading enzymes such as laccase can also degrade xenobiotic compounds. the effects of interspecific interactions between white-rot fungi and other microorganisms on laccase activity was studied in laboratory cultures. laccase activity in cultures of trametes versicolor and pleurotus ostreatus increased significantly after the introduction of soil fungi, bacteria and yeasts or after contact with nonsterile soil. addi ... | 2004 | 19712364 |
| influence of calcinated starfish powder on growth, yield, spawn run and primordial germination of king oyster mushroom (pleurotus eryngii). | this study was undertaken to assess the calcium absorption efficacy of pleurotus eryngii (king oyster mushroom) in sawdust medium supplemented with starfish powder and to determine the effect of starfish powder as calcium supplement on growth, yield, spawn run and primordial germination of p. eryngii. optimum calcination of starfish powder was achieved at the temperature ranging from 560.1 to 649.5 degrees c. a 1% supplementation of starfish powder in sawdust medium did not suppress the growth o ... | 2009 | 19733611 |
| random mutants of a pleurotus ostreatus laccase as new biocatalysts for industrial effluents bioremediation. | to select better performing laccase variants among the 2300 randomly mutated variants of pleurotus ostreatus poxa1b laccase to develop improved laccase-based biocatalysts. | 2010 | 19735323 |
| molecular identification of trichoderma species associated with pleurotus ostreatus and natural substrates of the oyster mushroom. | green mold of pleurotus ostreatus, caused by trichoderma species, has recently resulted in crop losses worldwide. therefore, there is an emerging need for rapid means of diagnosing the causal agents. a pcr assay was developed for rapid detection of trichoderma pleurotum and trichoderma pleuroticola, the two pathogens causing green mold of p. ostreatus. three oligonucleotide primers were designed for identifying these species in a multiplex pcr assay based on dna sequences within the fourth and f ... | 2009 | 19735461 |
| [determination of illudin s in omphalotus guepiniformis and foods that caused food poisoning by liquid chromatography with tandem mass spectrometry]. | a simple method was developed for determination of illudin s in fungi (omphalotus guepiniformis: poisonous mushroom) and a food that caused food poisoning, using liquid chromatography tandem mass spectrometry (lc/ms/ms). illudin s in fungi and the food that caused food poisoning was extracted with methanol and then cleaned up with an oasis hlb cartridge. lc separation was performed with an octadecylated silica column (inertsil ods-3, 2.1 mm i.d. x 150 mm) and a mobile phase of 0.1% formic acid-m ... | 2009 | 19745584 |
| effect of fungal pellet morphology on enzyme activities involved in phthalate degradation. | pellet size of white rot fungus, pleurotus ostreatus may affect the secretion of its degradative enzymes and accompanying biodegrading capability, but could be controlled by several physical culture conditions in liquid culture. the pellet size of p. ostreatus was affected by the volume of inoculum, flask, and medium, but the agitation speed was the most important control factor. at the lower agitation speed of 100 rpm, the large pellets were formed and the laccase activity was higher than that ... | 2009 | 19763415 |
| bioactive modification of silicon surface using self-assembled hydrophobins from pleurotus ostreatus. | a crystalline silicon surface can be made biocompatible and chemically stable by a self-assembled biofilm of proteins, the hydrophobins (hfbs) purified from the fungus pleurotus ostreatus. the protein-modified silicon surface shows an improvement in wettability and is suitable for immobilization of other proteins. two different proteins were successfully immobilized on the hfbs-coated chips: the bovine serum albumin and an enzyme, a laccase, which retains its catalytic activity even when bound o ... | 2009 | 19763645 |
| induction of metalloproteinase 9 secretion from human keratinocytes by pleuran (beta-glucan from pleurotus ostreatus). | glucan preparations, primarily modified water-soluble glucans, are involved in the activation of the body's natural defense mechanisms and in the acceleration of the skin's wound-healing processes. pleuran, an insoluble beta-d-glucan in hydrogel form, offers a natural alternative to more common chemically derivated soluble beta-d-glucans. pleuran was applied to human keratinocyte primary cultures, and after 24 h of incubation the release of matrix metalloproteinase 9 (mmp-9) and metalloproteinas ... | 2009 | 19791514 |
| biodegradation of crystal violet by low molecular mass fraction secreted by fungus. | biodegradation of crystal violet by white rot fungus pleurotus ostreatus bp in rice straw solid-state medium mostly depended on low molecular mass fraction (lmmf) and the hypothetical metabolic pathways of crystal violet by lmmf was proposed by the biodegradation of crystal violet and by the deduced six metabolites formed. | 2009 | 19804867 |
| development and characterization of new microsatellite markers for the oyster mushroom (pleurotus ostreatus). | we developed and characterized 36 polymorphic microsatellite markers for the oyster mushroom (pleurotus ostreatus). in total, 169 alleles were identified with an average of 4.7 alleles per locus. values for observed (ho) and expected (he) heterozygosities ranged from 0.027 to 0.946 and from 0.027 to 0.810, respectively. nineteen loci deviated from hardy-weinberg equilibrium. significant (p<0.05) excess heterozygosity was observed at nine loci. linkage disequilibrium (ld) was significant (p<0.05) ... | 2009 | 19809239 |
| isolation of antifungal compounds from gardenia jasminoides. | gardenia jasminoides e. (rubiaceae) methanol extracts showed the highest level of antifungal activity against pleurotus ostreatus, a wood-rotting fungus, compared to five other methanol plants extracts; [thuja orientalis l. (cupressaceae), datura innoxia (solanaceae), ligustrum japonicum t. (oleaceae), juniperus chinensis var. procumbens (cupressaceae) and mallotus japonica m. (euphorbiaceae)] and selected for further analysis. two antifungal compounds were isolated from n-butanol and ethyl acet ... | 2009 | 19817121 |
| [relationship between the laccase production of pleurotus ostreatus and the full wavelength scan for the fermentation liquid]. | laccase, widely distributed in fungi lacking high substrate specificity, plays an important role in lignin degradation in nature and environmental protection. in order to determine or estimate the laccase production during the fermentation of liquid media, the authors studied the full-length wave scan on the rough fermentation liquid of the pleurotus ostreatus, which produces laccase high. combined with the normal chemical method and diameter of the laccase and mycelium stain, which grew on the ... | 2009 | 19839329 |
| [the composition of volatile components of dry cepe and oyster mushroom]. | the composition of aroma compounds in dry cepe mushroom (boletis edulis fr.) and oyster mushroom (pleurotus ostreatus fr.) was studied using capillary gas chromatography and chromatography-mass spectrometry. in dry cepe, 53 volatile compounds were identified, and in dry oyster mushroom 41 compounds were identified. volatile organic substances with various functional groups formed the flavor of dry mushrooms. unsaturated alcohols and ketones with eight carbon atoms were responsible for the mushro ... | 2009 | 19845295 |
| original and residual phytotoxicity of olive mill wastewater revealed by fractionations before and after incubation with pleurotus ostreatus. | concentrations of dissolved organic carbon (doc) and total phenols (tp), and the phytotoxicity to cress (lepidium sativum l.) were determined for three molecular-sized fractions of olive mill wastewater (omw), <1000, 1000-5000, and >5000 da, before and after incubation with pleurotus ostreatus. the <1000-da fraction contained 82% of the total doc and 48% of the tp, and was the most phytotoxic. ethyl acetate separation of aqueous and solvent fractions showed that the aqueous fraction contained 93 ... | 2009 | 19904988 |
| purification and characteristics of an enzyme with both bilirubin oxidase and laccase activities from mycelium of the basidiomycete pleurotus ostreatus. | a homogenous enzyme with both bilirubin oxidase and laccase activities was isolated from a submerged culture of the basidiomycete pleurotus ostreatus mycelium and characterized. the yield of the enzyme was 127 microg/g dry biomass of the mycelium. the specific activity of the enzyme was 21 and 261 u/mg to bilirubin and to a laccase substrate abts, respectively. the intracellular phenol oxidase from the p. ostreatus mycelium was identified as bilirubin oxidase with the amino acid sequence highly ... | 2009 | 19916914 |
| degradation of polycyclic aromatic hydrocarbons by crude extracts from spent mushroom substrate and its possible mechanisms. | biodegradation of polycyclic aromatic hydrocarbons (pahs) by pure laccase has been reported, but the high cost limited its application in environmental bioremediation. here, we reported a study about pahs degradation by crude extracts (ces) containing laccase, which were obtained by extracting four spent mushroom (agaricus bisporus, pleurotus eryngii, pleurotus ostreatus, and coprinus comatus) substrates. the results showed that anthracene, benzo[a]pyrene, and benzo[a]anthracene were top three d ... | 2010 | 19924475 |
| cultivation of pleurotus ostreatus and other edible mushrooms. | pleurotus ostreatus is the second most cultivated edible mushroom worldwide after agaricus bisporus. it has economic and ecological values and medicinal properties. mushroom culture has moved toward diversification with the production of other mushrooms. edible mushrooms are able to colonize and degrade a large variety of lignocellulosic substrates and other wastes which are produced primarily through the activities of the agricultural, forest, and food-processing industries. particularly, p. os ... | 2010 | 19956947 |
| microbial community structure changes during oyster mushroom substrate preparation. | although oyster mushroom (pleurotus spp.) is a valuable food, cultivated worldwide on an industrial scale, still very little is known about the microbial dynamics during oyster mushroom substrate preparation. therefore, the characterization of the microbial dynamics by chemical and biological tools was the objective of this study. during substrate preparation, enzymatic digestibility of the substrate improved by 77%, whereas the cellulose and hemicellulose to lignin ratios decreased by 9% and 19 ... | 2010 | 19967354 |
| bioprocess of triphenylmethane dyes decolorization by pleurotus ostreatus bp under solid-state cultivation. | with an aim to evaluate dye decolorization by white rot fungus on natural living conditions, reproducing by solidstate fermentation, the process of triphenylmethane dyes decolorization using the white rot fungus p. ostreatus bp, cultivated on rice straw solid-state medium, has been demonstrated. three typical dyes, including malachite green, bromophenol blue, and crystal violet, were almost completely decolorized by the fungus after 9 days of incubation. during the process of dye decolorization, ... | 2009 | 19996697 |
| broiler performance on finisher diets containing different levels of either pleurotus ostreatus-fermented dried cocoa pod husk or dried cocoa pod husk supplemented with enzymes. | the purpose was to investigate the feeding value of fibrous cocoa pod husk (cph) either fermented with pleurotus ostreatus or supplemented with fibre-degrading multi-enzymes (viscozyme l + pectinex 5xl) before inclusion in diets for 3-week-old finishing broilers. growth performance and carcass yield of broilers fed diets containing 100, 200 or 300 g kg(-1) of the p. ostreatus-fermented cph or 100, 200 or 300 g kg(-1) of the enzyme-supplemented cph as well as a normal broiler finisher diet (contr ... | 2010 | 20012483 |
| the effects of β-glucan isolated from pleurotus ostreatus on methotrexate treatment in rats with adjuvant arthritis. | the purpose of this study was to evaluate the effect of β-(1,3/1,6)-d: -glucan isolated from pleurotus ostreatus (β-glucan-po) on prophylactic treatment of adjuvant arthritis (aa) with methotrexate (mtx) in rats. groups of rats with aa were treated with methotrexate (1 mg/kg/week), β-glucan-po (1 mg/kg every second day) or their combination for the period of 28 days from adjuvant application. body mass, hind paw swelling, arthrogram scores and a level of serum albumin were measured as markers of ... | 2011 | 20033413 |
| yield performances and changes in enzyme activities of pleurotus spp. (p. ostreatus and p. sajor-caju) cultivated on different agricultural wastes. | pleurotus ostreatus and pleurotus sajor-caju were studied for their ability to produce laccase and carboxymethylcellulase (cmcase) enzymes on different agricultural wastes under solid state fermentation. the spawns of p. ostreatus and p. sajor-caju were inoculated on different agricultural wastes including viticulture wastes, wheat straw, paddy straw, sesame straw, sawdust as well as the mixtures of these wastes with wheat bran. the carbon and nitrogen contents of substrates containing bran were ... | 2010 | 20056410 |
| [purification and characterization of milk-clotting enzymes from oyster mushroom (pleurotus ostreatus (fr.) kumm.)]. | three enzymes with milk-clotting activity have been isolated from the fruiting bodies of pleurotus ostreatus (fr.) kumm.) by (nh4)2so4 precipitation, gel chromatography on sephadex g75, and ion exchange chromatography on carboxymethylcellulose (cmc). isoelectric points of the enzymes, as determined by isoelectrofocusing, equaled 4.2, 6.7, and 8.8. inhibition analysis showed that the enzymes with isoelectric points of 4.2 and 6.7 belong to the class of metal-dependent proteinases, while the enzym ... | 2009 | 20067154 |
| the pleurotus ostreatus hydrophobin vmh2 and its interaction with glucans. | hydrophobins are small self-assembling proteins produced by fungi. a class i hydrophobin secreted by the basidiomycete fungus pleurotus ostreatus was purified and identified. the pure protein is not water soluble, whereas complexes formed between the protein and glycans, produced in culture broth containing amylose, are soluble in water. glycan structure matched to cyclic structures of alpha-(1-4) linked glucose containing from six to 16 monomers (cyclodextrins). moreover, it was verified that n ... | 2010 | 20100692 |
| effect of the polysaccharide extract from the edible mushroom pleurotus ostreatus against infectious bursal disease virus. | the polysaccharide-containing extracellular fractions (efs) of the edible mushroom pleurotus ostreatus have immunomodulating effects. being aware of these therapeutic effects of mushroom extracts, we have investigated the synergistic relations between these extracts and biavac and biaromvac vaccines. these vaccines target the stimulation of the immune system in commercial poultry, which are extremely vulnerable in the first days of their lives. by administrating ef with polysaccharides from p. o ... | 2009 | 20111675 |
| epr and ftir studies reveal the importance of highly ordered sterol-enriched membrane domains for ostreolysin activity. | ostreolysin is a cytolytic protein from the edible oyster mushroom (pleurotus ostreatus), which recognizes specifically and binds to raft-like sterol-enriched membrane domains that exist in the liquid-ordered phase. its binding can be abolished by micromolar concentrations of lysophospholipids and fatty acids. the membrane activity of ostreolysin, however, does not completely correlate with the ability of a certain sterol to induce the formation of a liquid-ordered phase, suggesting that the pro ... | 2010 | 20114031 |
| oyster mushroom laccase inhibits hepatitis c virus entry into peripheral blood cells and hepatoma cells. | there is no protective vaccine or effective drug against hepatitis c virus (hcv). sustained virological response to inf/ribavirin treatment regimen has an efficiency of about 50%. many patients worldwide have used traditional medicines and herbal medicine in particular. a laccase has been purified from oyster mushroom (pleurotus ostreatus) to homogeneity by deae affi-gel blue gel, cm-sephadex g-50 and sephadex g-100. the molecular weight of the laccase was about 58 kda in sds-page. the optimum p ... | 2010 | 20156183 |
| antimicrobial and antioxidant activities of mycelia of 10 wild mushroom species. | antimicrobial and antioxidant activities of mycelia obtained from 10 wild edible mushrooms-armillaria mellea, meripilus giganteus, morchella costata, morchella elata, morchella esculenta var. vulgaris, morchella hortensis, morchella rotunda, paxillus involutus, pleurotus eryngii, and pleurotus ostreatus-were investigated. for determination of antimicrobial activities of these mushrooms, ethanol extracts were examined with 11 test microorganisms by the agar well diffusion method. p. ostreatus and ... | 2010 | 20192845 |
| evaluation of holocellulase production by plant-degrading fungi grown on agro-industrial residues. | agaricus brasiliensis cs1, pleurotus ostreatus h1 and aspergillus flavus produced holocellulases when grown in solid and submerged liquid cultures containing agro-industrial residues, including sugar cane bagasse and dirty cotton residue, as substrates. these isolates proved to be efficient producers of holocellulases under the conditions used in this screening. bromatological analysis of agro-industrial residues showed differences in protein, fiber, hemicellulose, cellulose and lignin content. ... | 2010 | 20221846 |
| inoculum carrier and contaminant bioavailability affect fungal degradation performances of pah-contaminated solid matrices from a wood preservation plant. | the objective of the study was to investigate the impact of chopped wheat straw (cws), ground corn cobs (gcc) and commercial pellets (cp), as inoculum carriers, on both growth and polycyclic aromatic hydrocarbons (pah) degradation performances of dichomitus squalens, pleurotus ostreatus and coprinus comatus. a historically-contaminated soil (hcs) and creosote-treated shavings (cts) from the sobeslav wood preservation plant, characterized by different relative abundances of the pah bioavailable f ... | 2010 | 20299070 |
| [development of a laminar bioadsorbent with hypertolerant phanerochaete chrysosporium to cd, ni and pb for wastewater treatment]. | the use of basidiomycetes for metal removal is an alternative to traditional methods. in this, the biomass acts as a natural ionic exchanger removing metals from solution. | 2010 | 20346296 |
| differentially expressed genes under simulated microgravity in fruiting bodies of the fungus pleurotus ostreatus. | in response to a change in the direction of gravity, morphogenetic changes of fruiting bodies of fungi are usually observed as gravitropism. although gravitropism in higher fungi has been studied for over 100 years, there is no convincing evidence regarding the graviperception mechanism in mushrooms. to understand gravitropism in mushrooms, we isolated differentially expressed genes in pleurotus ostreatus (oyster mushroom) fruiting bodies developed under three-dimensional clinostat-simulated mic ... | 2010 | 20402784 |
| a semi-rational approach to engineering laccase enzymes. | in order to develop improved laccase-based bio-catalysts, semi-rational mutagenesis of the laccase poxa1b from pleurotus ostreatus was performed through a combination of directed evolution with elements of rational enzyme modification. the r4 laccase was prepared by joining mutations of previously selected poxa1b random variants. an enhancement of stability features was thus obtained, making the novel enzyme r4 more appropriate as scaffold for directed evolution. a library of 1000 randomly mutat ... | 2010 | 20467838 |
| effect of steam explosion pretreatment on treatment with pleurotus ostreatus for the enzymatic hydrolysis of rice straw. | the effects of steam explosion (1.5 mpa, 1 min) on the treatment of rice straw with pleurotus ostreatus were evaluated in terms of the change in composition of the components and the susceptibility to enzymatic hydrolysis. when rice straw was pretreated with a steam explosion prior to biological treatment, the treatment time required for obtaining a 33% net glucose yield was reduced to 36 days from 60 days. the reduction is probably due to loosening of networks of klason lignin with sugar moieti ... | 2010 | 20547367 |
| [comparisons of different methods for virus-elimination of edible fungi]. | four dsrna bands were extracted from pleurotus ostreatus td300 by the dsrna isolation technique with sizes of 8.2 kb, 2.5 kb, 2.1 kb, and 1.1 kb, respectively. four virus-eliminated methods, i. e. hyphal tips cut (htc), protoplast regeneration (pr), single spore hybridization (ssh), and frozen and lyophilized (fl), were applied to prepare virus-eliminated strains, and one virus-eliminated strain was selected for each virus-elimination method. the virus-eliminated strains were named as htc8, pr15 ... | 2010 | 20572349 |
| enzyme production by solid substrate fermentation of pleurotus ostreatus and trametes versicolor on tomato pomace. | a process of solid state fermentation (ssf) on tomato pomace was developed with the white-rot fungi pleurotus ostreatus and trametes versicolor, using sorghum stalks as support. operative parameters (humidity, water activity, and size of substrate particles) guaranteeing a good colonization of tomato pomace by both fungi were defined and conditions for production at high titers of the industrially relevant enzymes laccase, xylanase and protease were identified. significant laccase activity level ... | 2011 | 20582639 |
| electrostatically self-assembled azides on zinc sulfide nanoparticles as multifunctional nanoprobes for peptide and protein analysis in maldi-tof ms. | a simple method to synthesize electrostatically self-assembled azides on zinc sulfide nanoparticles (zns-n(3) nps) was described and then it was further applied as a multifunctional nanoprobe such as enriching, desalting, accelerating and separation-/washing free nanoprobes for rapid analysis of peptides and proteins and microwave assisted tryptic digested proteins in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms). the zns-n(3) nps were characterized ... | 2010 | 20602933 |
| differential gene expression of ligninolytic enzymes in pleurotus ostreatus grown on olive oil mill wastewater. | the expression of laccase and manganese peroxidase genes of a selected strain of pleurotus ostreatus were studied in olive oil mill wastewater (omw). the fungal strain decolourized 50% omw in a linear way for 21 days and, at the same time, degraded the phenol compounds by 85%. transcripts of laccase genes poxa1b, pox2, poxa3, and sspoxa3a, sspoxa3b coding for the small subunits of poxa3, were estimated by qrt-pcr, at different time intervals, together with beta-tubulin gene used as internal cont ... | 2010 | 20607227 |
| development of an efficient and simple method for conjugation of laccase to immunoglobulin and its characterization by enzyme immunoassay. | a new laccase-conjugated antibody is developed, containing laccase as an enzyme marker, obtained from culture supernatant of the basidiomycetous fungi pleurotus ostreatus. the efficacy of the laccase-conjugated antibody was demonstrated in indirect and direct enzyme immunoassay after using periodate and glutaraldehyde conjugation methods. the assay based on laccase-conjugated antibody is potent to detect antigens when compared with peroxidase conjugated antibody. | 2010 | 20623408 |
| characterization of pseudomonads isolated from decaying sporocarps of oyster mushroom. | pleurotus ostreatus is one of the most extensively cultivated mushrooms in the world; however, the success of cultivation often depends on the proliferation of different bacterial pathogens. pseudomonas tolaasii is thought as the major cause of brown blotch disease of agaricus bisporus and yellowing of pleurotus ostreatus. in this study we examined the pathogenicity and assessed the industrial damage causing effect of 41 pseudomonas strains isolated from deformed, yellowing oyster mushroom (p. o ... | 2011 | 20627228 |
| potential for bioremediation of agro-industrial effluents with high loads of pesticides by selected fungi. | wastewaters from the fruit packaging industry contain a high pesticide load and require treatment before their environmental discharge. we provide first evidence for the potential bioremediation of these wastewaters. three white rot fungi (wrf) (phanerochaete chrysosporium, trametes versicolor, pleurotus ostreatus) and an aspergillus niger strain were tested in straw extract medium (stem) and soil extract medium (sem) for degrading the pesticides thiabendazole (tbz), imazalil (imz), thiophanate ... | 2011 | 20635121 |
| both common and specialty mushrooms inhibit adhesion molecule expression and in vitro binding of monocytes to human aortic endothelial cells in a pro-inflammatory environment. | cardiovascular disease (cvd) is a leading cause of mortality in the united states as well as globally. epidemiological studies show that regular fruit and vegetable consumption reduces cvd risk, in part, due to antioxidant activity and immunomodulation since oxidative stress and inflammation are features of atherogenesis. accumulating evidence also shows that dietary fungi, viz., mushrooms, can protect against chronic disease by altering inflammatory environments such as those associated with cv ... | 2010 | 20637088 |
| isolation and characterization of a novel ribonuclease from the pink oyster mushroom pleurotus djamor. | a 15-kda rnase was purified from pleurotus djamor using ion exchange chromatography and gel filtration. its n-terminal amino acid sequence was different from previously reported rnase sequences of mushrooms belonging to the genus of pleurotus and other genera. the rnase exhibited maximal rnase activity at ph 4.6 and 60 degrees c. its activity toward polyhomoribonucleotides was poly(u) > poly(c) > poly(a) > poly(g). it inhibited proliferation of hepatoma cells and breast cancer cells. the ranking ... | 2010 | 20647680 |
| an extract of the pleurotus ostreatus mushroom bolsters the glutathione redox system in various organs of aged rats. | this study is to investigate the putative effect of an extract of the oyster mushroom, pleurotus ostreatus, on reduced glutathione (gsh) and its metabolic enzymes in major organs of male albino rats. a significant (p < .05) decrease in the level of gsh was observed in liver, kidneys, heart, and brain of aged rats compared to young rats. activities of glutathione s-transferase (gst), glutathione reductase (gr), and glucose 6-phosphate dehydrogenase (g6pdh) were significantly (p < .05) lower in th ... | 2010 | 20673055 |
| increasing pleurotus ostreatus laccase production by culture medium optimization and copper/lignin synergistic induction. | laccases have great biotechnological potential in diverse industries as they catalyze the oxidation of a broad variety of chemical compounds. production of laccases by basidiomycetes has been broadly studied as they secrete the enzymes, grow on cheap substrates, and they generally produce more than one isoenzyme (constitutive and/or inducible). laccase production and isoenzyme profile can be modified through medium composition and the use of inducers. the objective of this work was to increase l ... | 2011 | 20694851 |
| lecanicillium fungicola: causal agent of dry bubble disease in white-button mushroom. | lecanicillium fungicola causes dry bubble disease in commercially cultivated mushroom. this review summarizes current knowledge on the biology of the pathogen and the interaction between the pathogen and its most important host, the white-button mushroom, agaricus bisporus. the ecology of the pathogen is discussed with emphasis on host range, dispersal and primary source of infection. in addition, current knowledge on mushroom defence mechanisms is reviewed. taxonomy: lecanicillium fungicola (pr ... | 2010 | 20695998 |
| an extract of the oyster mushroom, pleurotus ostreatus, increases catalase gene expression and reduces protein oxidation during aging in rats. | the objective of the present study was to address the effect of mushroom pleurotus ostreatus on the catalase (cat) gene expression and the protein carbonyls in liver and kidney of aged (24 months old) rats. | 2010 | 20727333 |
| white-rot fungal conversion of wheat straw to energy rich cattle feed. | in order to improve the digestibility and nutrient availability in rumen, wheat straw was subjected to solid state fermentation (ssf) with white-rot fungi (i.e. pleurotus ostreatus and trametes versicolor) and the fermented biomass (called myco-straw) was evaluated for biochemical, enzymatic and nutritional parameters. the fungal treatment after 30 days led to significant decrease (p < 0.05) in cell wall constituents viz, acid detergent fiber (adf), neutral detergent fiber (ndf), hemicellulose, ... | 2010 | 20734121 |
| optimization of laccase production by pleurotus ostreatus imi 395545 using the taguchi doe methodology. | production of laccase using a submerged culture of pleurotus orstreatus imi 395545 was optimized by the taguchi orthogonal array (oa) design of experiments (doe) methodology. this approach facilitates the study of the interactions of a large number of variables spanned by factors and their settings, with a small number of experiments, leading to considerable savings in time and cost for process optimization. this methodology optimizes the number of impact factors and enables to calculate their i ... | 2010 | 20806254 |
| particle geometry affects differentially substrate composition and enzyme profiles by pleurotus ostreatus growing on sugar cane bagasse. | the growth of pleurotus ostreatus was analyzed on three particle sizes of sugar cane bagasse: 0.92 mm and 1.68 mm in diameter, in addition to heterogeneous fibers (average 2.9 mm in diameter). specific growth rate on heterogeneous particles was lower (μ=0.043 h(-1)), although soluble protein production was maximal (809 μg/g dry wt). higher μ values were reached on the other two particles sizes (0.049-0.05 h(-1)) with less soluble protein (500 μg/g dry wt). xylanases and laccases were favored in ... | 2011 | 20846859 |
| induction and purification by three-phase partitioning of aryl alcohol oxidase (aao) from pleurotus ostreatus. | aryl alcohol oxidase (aao) is an extracellular flavoenzyme involved in lignin degradation by white rot fungi. screening of lignolytic and aao activity from twenty different fungal species were carried out. among them, seven species showed lignolytic activity and three of them (pleurotus ostreatus, pleurotus eous, and pleurotus platypus) were found to be aao positive. maximal aao activity was observed in batch cultures of p. ostreatus and was found to be induced by aromatic amino acids and aryl a ... | 2011 | 20857231 |
| agrobacterium tumefaciens mediated fused egfp-hph gene expression under the control of gpd promoter in pleurotus ostreatus. | a transformation system for the basidiomycete pleurotus ostreatus was established using agrobacterium-mediated infection. following p. ostreatus glyceraldehyde-3-phosphate dehydrogenase gene analysis, its promoter region including two introns was used as cis-regulatory element to drive expression of enhanced green fluorescent protein (egfp). as a selection marker, the hygromycin phosphotransferase (hph) gene cassette was used in the binary vector ppeh. mycelia without pretreatment were found to ... | 2010 | 20869218 |
| mitigation of arsenic-mediated renal oxidative stress in rat by pleurotus florida lectin. | oyster mushroom, pleurotus florida is regarded as one of the popular food with biopharmaceutical properties. here, the study aimed to investigate the antioxidative effects of mushroom (pleurotus florida) lectin against arsenic-induced nephrotoxicity in rats. animals were divided into four groups; group 1 was control. groups 2, 3 and 4 were exposed to arsenic (20 parts per million [ppm] in drinking water), arsenic plus oral supplementation of ascorbic acid (25 mg/kg body weight) and arsenic plus ... | 2010 | 20876158 |
| pleurotus ostreatus inhibits colitis-related colon carcinogenesis in mice. | colorectal cancer is one of the leading causes of cancer deaths in both men and women in the world. however, colon cancer can be prevented to some extent by consumption of edible natural products with chemopreventive properties. therefore, we investigated, whether edible mushroom pleurotus ostreatus (po) has chemopreventive effect on inflammation-associated colon carcinogenesis induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip) and promoted by dextran sodium sulfate (dss). po trea ... | 2010 | 20878085 |
| medicinal and edible lignicolous fungi as natural sources of antioxidative and antibacterial agents. | the antioxidant activity of organic extracts of eight fungal species, ganoderma lucidum, ganoderma applanatum, meripilus giganteus, laetiporus sulphureus, flammulina velutipes, coriolus versicolor, pleurotus ostreatus and panus tigrinus, was evaluated for free radical (dpph· and oh·) scavenging capacity and an effect on lipid peroxidation, and the antibacterial activity was tested by the agar well diffusion method. the highest dpph· scavenging activity was found in the methanol extract of g. app ... | 2010 | 20878697 |
| commonly consumed and specialty dietary mushrooms reduce cellular proliferation in mcf-7 human breast cancer cells. | worldwide, over one million women will be newly diagnosed with breast cancer in the next year. moreover, breast cancer is the second leading cause of cancer death in the usa. an accumulating body of evidence suggests that consumption of dietary mushrooms can protect against breast cancer. in this study, we tested and compared the ability of five commonly consumed or specialty mushrooms to modulate cell number balance in the cancer process using mcf-7 human breast cancer cells. hot water extracts ... | 2010 | 20921274 |
| removal of neutral red from aqueous solution by adsorption on spent cottonseed hull substrate. | cottonseed hull, a low-cost widely available agricultural waste in china, after used as substrate for the white rot fungus pleurotus ostreatus cultivation, was tested for the removal of neutral red (nr), a cationic dye, from aqueous solution. a batch adsorption study was carried out with varied solution ph, adsorbent dosage, reaction time and initial nr concentration. the results show that the kinetics of dye removal by the spent cottonseed hull substrate (schs) is prompt in the first 5 min and ... | 2011 | 20933326 |
| particle and naked rna mycoviruses in industrially cultivated mushroom pleurotus ostreatus in china. | many cultivated mushroom strains, such as pleurotus ostreatus td300, displayed symptoms of degeneration. a spherical virus posv and four dsrna segments were extracted from mycelium of p. ostreatus td300. posv had a diameter of 23 nm and encapsidated a 2.5kb dsrna segment with coat proteins whose molecular weights were 39 kda and 30 kda. four dsrna segments were 8.2 kb, 2.5 kb, 2.0 kb, and 1.1 kb in size, respectively. the 1.1 kb dsrna segment often escaped detection. the cdna and the amino acid ... | 2010 | 20943162 |
| identification of a new member of pleurotus ostreatus laccase family from mature fruiting body. | laccases (benzenediol:oxygen oxidoreductases, ec 1.10.3.2) are blue multicopper oxidases, catalyzing the oxidation of an array of aromatic substrates concomitantly with the reduction of molecular oxygen to water. most of the known laccases have fungal or plant origins, although few laccases have been also identified in bacteria and insects. most of the fungal laccases reported thus far are extra-cellular enzymes, whereas only few enzymes from fruiting bodies have been described so far. multiple ... | 2010 | 20943181 |
| identification of blue-light photoresponse genes in oyster mushroom mycelia. | using the characteristics of light-emitting diodes, we studied the effects of light quality, light intensity, and repeated light-dark cycles on the growth of oyster mushroom mycelia. blue-light stimulation suppressed growth in oyster mushroom mycelia, and this effect was remarkably dependent on light intensity. random amplified polymorphic dna differential display analysis enabled the identification for the first time of 15 upregulated and 13 downregulated genes induced by blue-light stimulation ... | 2010 | 20944395 |