| gene cloning, expression, and characterization of trehalose-6-phosphate synthase from pleurotus ostreatus. | trehalose-6-phosphate synthase (tps; ec2.4.1.15) catalyzes the first step in trehalose synthesis, which involves transfer of glucose from uridine diphosphate glucose (udpg) to glucose 6-phosphate (g6p) to form trehalose-6-phosphate. to determine the gene and enzymatic characteristics of tps in pleurotus ostreatus, we cloned and sequenced the cdna of potps1, which contains a 1665 bp open reading frame that encodes a 554-amino acid protein with a predicted molecular weight of 62.01 kda. this gene ... | 2017 | 28513878 |
| biodegradation of ciprofloxacin by white rot fungus pleurotus ostreatus. | unrestricted and reckless use of antibiotics has resulted in their accumulation in environment. this, in turn, has led to the emergence of multiple drug-resistant microbes. the present study focuses on degradation of ciprofloxacin (cip) by an edible white rot fungus pleurotus ostreatus. effect of cip was determined on radial growth and biomass of p. ostreatus. titrimetric and spectrophotometric assays were carried out to assess the degrading potential of p. ostreatus towards cip. it was found th ... | 2017 | 28452015 |
| mushroom lectin overcomes hepatitis b virus tolerance via tlr6 signaling. | currently, chronic hepatitis b virus (hbv) infection remains a serious public health problem in the world. recombinant hbv vaccine, as a preventive strategy against hbv infection, generates high antibody level, but it is not effective to activate innate and cellular immunity for chronic hbv infection therapy. lectins from mushroom are natural and active proteins which have been shown important biological functions. however, little is known about the immunological mechanism engaged by mushroom le ... | 2017 | 28724955 |
| regulation of primary metabolic pathways in oyster mushroom mycelia induced by blue light stimulation: accumulation of shikimic acid. | shikimic acid is a key intermediate in the aromatic amino acid pathway as well as an important starting material for the synthesis of tamiflu, a potent and selective inhibitor of the neuraminidase enzyme of influenza viruses a and b. here we report that in oyster mushroom (pleurotus ostreatus) mycelia cultivated in the dark, stimulation with blue light-emitting diodes induces the accumulation of shikimic acid. an integrated analysis of primary metabolites, gene expression and protein expression ... | 2015 | 25721093 |
| use of solid digestate for lignocellulolytic enzymes production through submerged fungal fermentation. | studies were performed on the use of the solid fraction of digestate (d) for the production of lignocellulolytic enzymes (endo- and exo-glucanase, xylanase, β-glucosidase and laccase) by fungi, in comparison with wheat straw (benchmark) (w). to date, this is the first report on the use of such an inexpensive substrate in a liquid environment. submerged instead of solid state fermentation was applied to overcome ph inhibition and increase surface accessibility. a total of 21 fungal strains were t ... | 2017 | 28521209 |
| chemical composition and character impact odorants in volatile oils from edible mushrooms. | the aim of this study was to investigate the chemical composition and the odor-active components of volatile oils from three edible mushrooms, pleurotus ostreatus, pleurotus eryngii, and pleurotus abalonus, which are well-known edible mushrooms. the volatile components in these oils were extracted by hydrodistillation and identified by gc/ms, gc-olfactometry (gc-o), and aroma extract dilution analysis (aeda). the oils contained 40, 20, and 53 components, representing 83.4, 86.0, and 90.8% of the ... | 0 | 26567951 |
| biotransformation of 1,8-cineole by solid-state fermentation of eucalyptus waste from the essential oil industry using pleurotus ostreatus and favolus tenuiculus. | biotechnological conversion of low-cost agro-industrial by-products, such as industrial waste or terpenes from the distillation of essential oils from plants into more valuable oxygenated derivatives, can be achieved by using microbial cells or enzymes. in argentina, the essential oil industry produces several tons of waste each year that could be used as raw materials in the production of industrially relevant and value-added compounds. in this study, 1,8-cineole, one of the components remainin ... | 0 | 26342920 |
| occupational asthma in a fruit and vegetables vendor. | this study presents a case report of a woman running a fruit and vegetables market stall, occupationally exposed to mushroom spores, who developed asthmatic symptoms. basidiospores are allergens that can give rise to sensitization and subsequently trigger an allergic reaction, such as contact dermatitis, rhino-conjunctivitis, asthma and hypersensitivity pneumonitis. | 0 | 27015024 |
| iron translocation in pleurotus ostreatus basidiocarps: production, bioavailability, and antioxidant activity. | translocation of minerals from substrate to mushrooms can change the medicinal characteristics, commercial value, and biological efficiency of mushroom. in the present study, we demonstrated that addition of iron to the substrate reduces the yield of pleurotus ostreatus mushroom. the biological efficiency of the mushroom varied from 36.53% on the unsupplemented substrate to 2.08% for the substrate with 500 mg/kg iron added. the maximum iron concentration obtained for mushroom was 478.66 mg/kg (d ... | 0 | 26909996 |
| pressurized water extraction of β-glucan enriched fractions with bile acids-binding capacities obtained from edible mushrooms. | a pressurized water extraction (pwe) method was developed in order to extract β-glucans with bile acids-binding capacities from cultivated mushrooms (agaricus bisporus, lentinula edodes, and pleurotus ostreatus) to be used as supplements to design novel foods with hypocholesterolemic properties. extraction yields were higher in individual than sequential extractions being the optimal extraction parameters: 200°c, 5 cycles of 5 min each at 10.3 mpa. the crude polysaccharide (psc) fractions, isola ... | 0 | 24399760 |
| olive mill wastewater biodegradation potential of white-rot fungi--mode of action of fungal culture extracts and effects of ligninolytic enzymes. | forty-nine white-rot strains belonging to 38 species of basidiomycota were evaluated for olive-mill wastewater (omw) degradation. almost all fungi caused high total phenolics (>60%) and color (⩽ 70%) reduction, while cod and phytotoxicity decreased to a lesser extent. culture extracts from selected agrocybe cylindracea, inonotus andersonii, pleurotus ostreatus and trametes versicolor strains showed non-altered physicochemical and enzymatic activity profiles when applied to raw omw in the presenc ... | 2015 | 25879179 |
| bioconversion of lignocellulosic residues by agrocybe cylindracea and pleurotus ostreatus mushroom fungi--assessment of their effect on the final product and spent substrate properties. | nine agro-industrial and forestry by-products were subjected to solid-state fermentation by agrocybe cylindracea and pleurotus ostreatus, and the process and end-products were comparatively evaluated. grape marc waste plus cotton gin trash was the best performing medium for both fungi, while substrate composition had a marked effect on most cultivation parameters. biological efficiency was positively correlated with nitrogen, lignin and ash, and negatively with hemicelluloses and carbohydrate co ... | 2014 | 24837930 |
| enzymatic formation of compound-k from ginsenoside rb1 by enzyme preparation from cultured mycelia of armillaria mellea. | minor saponins or human intestinal bacterial metabolites, such as ginsenosides rg3, f2, rh2, and compound k, are more pharmacologically active than major saponins, such as ginsenosides rb1, rb2, and rc. in this work, enzymatic hydrolysis of ginsenoside rb1 was studied using enzyme preparations from cultured mycelia of mushrooms. | 2016 | 27158230 |
| market survey and risk assessment for trace metals in edible fungi and the substrate role in accumulation of heavy metals. | levels of cadmium (cd), arsenic (as), mercury (hg), lead (pb), iron (fe), and zinc (zn) were investigated in 285 samples of 9 species of edible fungi (lentinus edodes, auricularia auricula, pleurotus ostreatus, tremella fuciformis, flammulina velutipes, agrocybe chaxinggu, armillaria mellea, agaricus bisporus, and pholiota nameko), which were collected from markets in beijing, china. in addition, edible fungi and culture substrates were collected from 7 cultivation bases to examine the role of t ... | 2015 | 26077507 |
| purification and characterization of a novel fibrinolytic enzyme from culture supernatant of pleurotus ostreatus. | a fibrinolytic enzyme was produced by an edible mushroom of pleurotus ostreatus using submerged culture fermentation. the enzyme was purified from the culture supernatant by applying a combination of freeze-thaw treatment, ammonium sulfate precipitation, hydrophobic interaction, and gel filtration chromatographies. the enzyme was purified by a 147-fold, with a yield of 7.54%. the molecular masses of the enzyme an determined by gel filtration and sdspage were 13.6 and 18.2 kda, respectively. the ... | 2014 | 24248089 |
| molybdophyllysin, a toxic metalloendopeptidase from the tropical toadstool, chlorophyllum molybdites. | a toxic protein, dubbed molybdophyllysin, was isolated from the tropical toadstool chlorophyllum molybdites by following its lethal effect in mice. analysis of the protein using sds-page revealed a single 23-kda band. sequence analysis of molybdophyllysin tryptic fragments showed that this protein is highly homologous to metalloendopeptidases (meps) obtained from edible mushrooms, such as grifola frondosa, pleurotus ostreatus, and armillaria mellea. these proteins include a hexxh+d zinc-binding ... | 2012 | 23063519 |
| analysis of indole compounds in edible basidiomycota species after thermal processing. | methanolic extracts of processed fruiting bodies of six edible mushroom species (basidiomycota) -armillaria mellea, boletus badius, boletus edulis, cantharellus cibarius, lactarius deliciosus collected from natural habitats and pleurotus ostreatus of commercial origin - were analysed for the presence of non-hallucinogenic indole compounds. thermal processing was designed in such a way that it mimicked conditions used for cooking of mushroom dishes, since only a narrow group of mushrooms can be e ... | 2012 | 26434315 |
| the lysine-specific proteinase from armillaria mellea is a member of a novel class of metalloendopeptidases located in basidiomycetes. | the fruiting body of the basidiomycete fungus armillaria mellea produces a lysine-specific proteinase which exhibits both potent fibrinolytic activity and a remarkable resistance to denaturing agents. an improved purification protocol has been developed for this enzyme and the sequence of the 26 n-terminal amino acid residues of the pure protein has been determined by gas-phase sequencing. searches of the swissprot database showed that the n-terminal sequence of a. mellea proteinase is highly si ... | 1999 | 10448068 |
| simple screening protocol for identification of potential mycoremediation tools for the elimination of polycyclic aromatic hydrocarbons and phenols from hyperalkalophile industrial effluents. | a number of fungal strains belonging to the ascomycota, basidiomycota and zygomycota genera were subjected to an in vitro screening regime to assess their ligninolytic activity potential, with a view to their potential use in mycoremediation-based strategies to remove phenolic compounds and polycyclic aromatic hydrocarbons (pahs) from industrial wastewaters. all six basidiomycetes completely decolorized remazol brilliant blue r (rbbr), while also testing positive in both the guaiacol and gallic ... | 2017 | 28499155 |
| evaluation of the chemical and antioxidant properties of wild and cultivated mushrooms of ghana. | knowledge of the chemical composition of both wild and cultivated edible mushrooms in ghana is limited. this study reports their nutritional value, composition in lipophilic and hydrophilic molecules, minerals and antioxidant properties. the samples were found to be nutritionally rich in carbohydrates, ranging from 64.14 ± 0.93 g in pleurotus ostreatus strain em-1 to 80.17 ± 0.34 g in lentinus squarosullus strain lsf. the highest level of proteins (28.40 ± 0.86 g) was recorded in the mentioned p ... | 2014 | 25432007 |
| release of feruloylated oligosaccharides from wheat bran through submerged fermentation by edible mushrooms. | wheat bran, a by-product of the flour industry, is believed to be a raw material for the production of feruloylated oligosaccharides (fos) because of its high content of conjiont ferulic acid (fa). studies were carried out to identify edible mushrooms that are able to release fos from wheat bran. all the six tested mushrooms (pleurotus ostreatus, hericium erinaceum, auricularia auricula, cordyceps militaris, agrocybe chaxingu, and ganoderma lucium) were found to release fos, and agrocybe chaxing ... | 2014 | 24123163 |
| mycelium growth kinetics and optimal temperature conditions for the cultivation of edible mushroom species on lignocellulosic substrates. | the influence of environmental parameters on mycelial linear growth of pleurotus ostreatus, p. eryngii, p. pulmonarius, agrocybe aegerita, lentinula edodes, volvariella volvacea and auricularia auricula-judae was determined in two different nutrient media in a wide range of temperature, forming the basis for the assessment of their temperature optima. v. volvacea grew faster at 35 degrees c, p. eryngii at 25 degrees c, p. ostreatus and p. pulmonarius at 30 degrees c, a. aegerita at 25 or 30 degr ... | 2001 | 11702409 |
| biodegradation of polycyclic aromatic hydrocarbons (pahs) by fungal enzymes: a review. | polycyclic aromatic hydrocarbons (pahs) are a large group of chemicals. they represent an important concern due to their widespread distribution in the environment, their resistance to biodegradation, their potential to bioaccumulate and their harmful effects. several pilot treatments have been implemented to prevent economic consequences and deterioration of soil and water quality. as a promising option, fungal enzymes are regarded as a powerful choice for degradation of pahs. phanerochaete chr ... | 2017 | 28115152 |
| biodegradation of 2-naphthalensulfonic acid polymers by white-rot fungi: scale-up into non-sterile packed bed bioreactors. | this paper presents a first scale up under non-sterile conditions of the biodegradation process of 2-naphthalensulfonic acid polymers (nsap) contained in a petrochemical wastewater by two white-rot fungi (bjerkandera adusta and pleurotus ostreatus). the biodegradation experiment was conducted first in flasks and then in packed-bed bioreactors filled with inert and biodegradable carriers (straw), the latter acting as both physical support and carbon source. reactor inoculated with p. ostreatus at ... | 2016 | 27587355 |
| biodegradation of naphthalenesulphonate polymers: the potential of a combined application of fungi and bacteria. | the potential of several fungi and their synergy with bacterial biomasses were evaluated as a solution for the removal of 2-naphthalensulphonic acid polymers (2-nsaps) from petrochemical wastewater, characterized by a chemical oxygen demand (cod) greater than 9000 mg/l. the ability of fungi to grow on 2-nsap mixtures was preliminarily investigated using a solid medium, and then the action of the selected strains, both in suspended and immobilized form, was evaluated in terms of degradation, depo ... | 2014 | 25351603 |
| involvement of the ligninolytic system of white-rot and litter-decomposing fungi in the degradation of polycyclic aromatic hydrocarbons. | polycyclic aromatic hydrocarbons (pahs) are natural and anthropogenic aromatic hydrocarbons with two or more fused benzene rings. because of their ubiquitous occurrence, recalcitrance, bioaccumulation potential and carcinogenic activity, pahs are a significant environmental concern. ligninolytic fungi, such as phanerochaete chrysosporium, bjerkandera adusta, and pleurotus ostreatus, have the capacity of pah degradation. the enzymes involved in the degradation of pahs are ligninolytic and include ... | 2012 | 22830035 |
| [about different mechanisms of the biochemical adaptation of mycelial fungi to temperature stress: changes in cytosol carbohydrate composition]. | the effect of temperature stress on the cytosol carbohydrate composition of fungi belonging to various systematic groups was investigated. in mucorales representatives (subkingdom eomycota, phylum archemycota, class zygomycetes), adaptation to hypo- and hyperthermia occurs via the regulation of trehalose synthesis, although inositol is also involved in these processes in blakeslea trispora. basidiomycota (subkingdom neomycota) use two pathways of biochemical adaptation, depending on the cytosol ... | 2006 | 11314645 |
| storage temperature and uv-irradiation influence on the ergosterol content in edible mushrooms. | ergosterol (5,7,22-ergostatrien-3β-ol) and ergosteryl derivatives from different genera of edible mushrooms were separated and quantified by an isocratic reversed-phase high performance liquid chromatography (hplc) method. the technique allowed a rapid separation of free ergosterol and two ergosteryl derivatives occurring in mushrooms. the ergosterol content varied considerably depending on the fungus. thus, the species agaricus bisporus and hygrophorus marzuolus presented high quantities of erg ... | 2014 | 24206714 |
| nutritional values and antioxidant potential of some edible mushrooms of kashmir valley. | mushrooms are considered rich in proteins, carbohydrates and other nutrients. the present study was carried out to evaluate some edible mushrooms of kashmir valley for their protein, carbohydrate and lipid contents. the highest protein content was found in boletus edulis (2.20g) followed by agaricus bisporus (1.80g), pleurotus ostreatus (1.68g), morchella esculenta (1.62g) and pleurotus sajor caju (1.6g). carbohydrate content also showed variation in all the five tested edible mushroom species, ... | 2012 | 22713951 |
| fatty acid compositions of six wild edible mushroom species. | the fatty acids of six wild edible mushroom species (boletus reticulatus, flammulina velutipes var. velutipes, lactarius salmonicolor, pleurotus ostreatus, polyporus squamosus, and russula anthracina) collected from different regions from anatolia were determined. the fatty acids were identified and quantified by gas chromatography and studied using fruit bodies. fatty acid composition varied among species. the dominant fatty acid in fruit bodies of all mushrooms was cis-linoleic acid (18 : 2). ... | 2013 | 23844377 |
| a comprehensive review of tropical milky white mushroom (calocybe indica p&c). | a compressive description of tropical milky white mushroom (calocybe indica p&c var. apk2) is provided in this review. this mushroom variety was first identified in the eastern indian state of west bengal and can be cultivated on a wide variety of substrates, at a high temperature range (30~38℃). however, no commercial cultivation was made until 1998. krishnamoorthy 1997 rediscovered the fungus from tamil nadu, india and standardized the commercial production techniques for the first time in the ... | 2015 | 26539033 |
| nutritional analysis of cultivated mushrooms in bangladesh - pleurotus ostreatus, pleurotus sajor-caju, pleurotus florida and calocybe indica. | mushroom cultivation has been started recently in bangladesh. awareness of the nutritional and medicinal importance of mushrooms is not extensive. in this study, the nutritional values of dietary mushrooms- pleurotus ostreatus, pleurotus sajorcaju, pleurotus florida and calocybe indica that are very popular among the cultivated mushrooms in bangladesh have been determined. these mushrooms were rich in proteins (20~25%) and fibers (13~24% in dry samples) and contained a lower amount of lipid (4 t ... | 2008 | 23997631 |
| characterization of vitamin b₁₂compounds in the wild edible mushrooms black trumpet (craterellus cornucopioides) and golden chanterelle (cantharellus cibarius). | this study determined the vitamin b₁₂ content of six wild edible mushrooms which are consumed by european vegetarians. zero or trace levels (0.01-0.09 µg/100 g dry weight) of vitamin b₁₂ were determined in porcini mushrooms (boletus spp.), parasol mushrooms (macrolepiota procera), oyster mushrooms (pleurotus ostreatus), and black morels (morchella conica). by contrast, black trumpet (craterellus cornucopioides) and golden chanterelle (cantharellus cibarius) mushrooms contained considerable level ... | 2012 | 23419403 |
| vitamin d4 in mushrooms. | an unknown vitamin d compound was observed in the hplc-uv chromatogram of edible mushrooms in the course of analyzing vitamin d(2) as part of a food composition study and confirmed by liquid chromatography-mass spectrometry to be vitamin d(4) (22-dihydroergocalciferol). vitamin d(4) was quantified by hplc with uv detection, with vitamin [(3)h] itamin d(3) as an internal standard. white button, crimini, portabella, enoki, shiitake, maitake, oyster, morel, chanterelle, and uv-treated portabella mu ... | 2012 | 22870201 |
| antigenic/allergenic analysis of basidiomycete cap, mycelia, and spore extracts. | since airborne basidiospores may be important inducers of respiratory allergy, extracts of spores, caps and mycelia from pleurotus ostreatus were studied by immunologic techniques. crossed immunoelectrophoresis of the pleurotus spore extract showed it to be a complex mixture containing at least 27 precipitating antigens. crossed-line immunoelectrophoresis comparing pleurotus spore extract with extracts of pleurotus cap or mycelia demonstrated both common antigens and antigens unique to the spore ... | 1987 | 3623711 |
| immobilization of defined laccase combinations for enhanced oxidation of phenolic contaminants. | immobilization is an important method to increase enzyme stability and allow enzyme reuse. one interesting application in the field of environmental biotechnology is the immobilization of laccase to eliminate phenolic contaminants via oxidation. fumed silica nanoparticles have interesting potential as support material for laccase immobilization via sorption-assisted immobilization in the perspective of applications such as the elimination of micropollutants in aqueous phases. based on these fact ... | 2014 | 23812279 |
| adsorption characteristics of copper (ⅱ), zinc (ⅱ) and mercury (ⅱ) by four kinds of immobilized fungi residues. | this study investigated the adsorption characteristics of copper (ⅱ), zinc (ⅱ) and mercury (ⅱ) by immobilized flammulina velutipes, auricularia polytricha, pleurotus eryngii and pleurotus ostreatus residues. lagergren model, elovich and intraparticle diffusion model were used to present the adsorption kinetics, and it was proved that langmuir isotherm model and pseudo-second order kinetics are the best suitable model with high correlation coefficient to characterize the adsorption process of cop ... | 2017 | 28865349 |
| occurrence of the (2r,3s)-isomer of 2-amino-3,4-dihydroxybutanoic acid in the mushroom hypsizygus marmoreus. | here, we report the occurrence of the (2r,3s)-isomer of 2-amino-3,4-dihydroxybutanoic acid (d-adhb) in the fruiting body of an edible mushroom, hypsizygus marmoreus. this is an unusual example of the accumulation of a d-amino acid whose enantiomer is not a proteinogenic amino acid. we show that d-adhb occurs specifically in the mushroom h. marmoreus. other edible mushrooms examined, including pholiota microspora, pleurotus eryngii, mycena chlorophos, sparassis crispa, grifola frondosa, pleurotus ... | 2017 | 28686838 |
| temperature-dependent development and reproductive traits of tyrophagus putrescentiae (sarcoptiformes: acaridae) reared on different edible mushrooms. | china is the largest producer, consumer, and exporter of mushrooms in the world. the storage mite, tyrophagus putrescentiae schrank, is one of the most important arthropod pests in mushroom cultivation. this study investigated the development and reproductive traits of this mite reared on four mushroom species: agaricus bisporus lange, pleurotus ostreatus kumm, auricularia polytricha (mont.) sacc., and flammulina velutipes (fr.) sing., at seven constant temperatures ranging from 16 to 34 °c at 8 ... | 2015 | 26313193 |
| evaluation of umami taste in mushroom extracts by chemical analysis, sensory evaluation, and an electronic tongue system. | seventeen edible mushrooms commercially available in korea were analysed for their umami taste compounds (5'-nucleotides: amp, gmp, imp, ump, xmp; free amino acids: aspartic, glutamic acid) and subjected to human sensory evaluation and electronic tongue measurements. amanita virgineoides featured the highest total 5'-nucleotide content (36.9 ± 1.50 mg/g), while monosodium glutamate-like components (42.4 ± 6.90 mg/g) were highest in agaricus bisporus. the equivalent umami concentration (euc) rang ... | 2016 | 26304449 |
| anti-inflammatory effects of five commercially available mushroom species determined in lipopolysaccharide and interferon-γ activated murine macrophages. | inflammation is a well-known contributing factor to many age-related chronic diseases. one of the possible strategies to suppress inflammation is the employment of functional foods with anti-inflammatory properties. edible mushrooms are attracting more and more attention as functional foods since they are rich in bioactive compounds, but their anti-inflammatory properties and the effect of food processing steps on this activity has not been systematically investigated. in the present study, whit ... | 2014 | 24262531 |
| isolation of high quality and yield of rna from agaricus bisporus with a simple, inexpensive and reliable method. | a method for isolating high purity and quantity rna from agaricus bisporus which is rich in proteins, carbohydrate, fiber and secondary metabolites, is described. rna was extracted from mycelium, primordia, sporophores at two development stages and two post-harvest storage stages as well as from pileipellis, inner cap, gill and stipe of the mature sporophore. the a(260)/a(230) and a(260)/a(280) ratios of isolated rna from fruiting bodies were both ~2 and the yield was about 200 μg/g fresh wt (fw ... | 2012 | 22447099 |
| [features of lipogenesis of basidiomycetes pleurotus ostreatus and flammulina velutipes in cultures on various media]. | the compositions of free fatty acids (fa) in the mycelia of oyster cap (pleurotus ostreatus (jacq. ex fr.) kumm.) and honey mushroom (flammulina velutipes (curt. ex fr.) sing.) and the effect of mycelium cultivation conditions on the composition and proportions of individual fa were investigated. palmitic and linoleic acids were found to be major acids produced by p. ostreatus growing on solid agar medium and in a submerged culture with a synthetic medium. the composition of minor fa in p. ostre ... | 2006 | 12325297 |
| [biological effect of space flight on edible fungi onboard recoverable scientific satellite]. | in order to breed good variety of edible fungi and provide data of cultivation of edible fungi in controlled ecological life support system (celss) in the future, 2 species of the edible fungi (pleurotus ostreatus and flammulina velutipes )were carried into space by a recoverable scientific satellite. a series of biological tests and cultivation experiments were done with the mycelia and sporocarp of these edible fungi. it was demonstrated that the edible fungi recovered from the satellite have ... | 1998 | 11543241 |
| lignin degradation by selected fungal species. | as biological decomposition of plant biomass represents a popular alternative environmental-friendly and economically justified process, screening of ligninolytic enzyme systems of various fungal species is a topical study area. the goal of the study was to obtain clear insight into the dynamics of laccase, mn-dependent peroxidase, and mn-independent peroxidase activity and levels of wheat straw lignin degradation in seven wood-rotting fungi. the best laccase producers were pleurotus ostreatus a ... | 2013 | 23612169 |
| valorization of spent oyster mushroom substrate and laccase recovery through successive solid state cultivation of pleurotus, ganoderma, and lentinula strains. | spent mushroom substrate (sms) of pleurotus ostreatus was supplemented with wheat bran and soybean flour in various proportions to obtain c/n ratios of 10, 20, and 30, and their effect was evaluated in successive cultivation of pleurotus ostreatus, pleurotus pulmonarius, ganoderma adspersum, ganoderma resinaceum, and lentinula edodes strains with respect to mycelium growth rate, biomass concentration, recovery of the enzyme laccase and crude exopolysaccharides, and also with additional fruiting ... | 2017 | 28361237 |
| levels of platinum group elements and rare-earth elements in wild mushroom species growing in poland. | due to limited data-describing abilities of mushrooms to accumulate platinum group elements (pges) and rare-earth elements (rees), the aim of this study was to determine, by inductively coupled plasma optical emission spectrometry followed by microwave-assisted sample digestion by nitric acid, the content of these elements in 20 mushroom species (10 above ground and 10 growing on wood), mostly edible, collected near a busy trunk road. the highest content of pges in above-ground mushroom species ... | 2016 | 26515437 |
| malachite green and crystal violet decolorization by ganoderma lucidum and pleurotus ostreatus supernatant and by rgllcc1 and rpoxa 1b concentrates: molecular docking analysis. | laccases catalyze the oxidation of various aromatic organic compounds concomitantly with molecular oxygen reduction to water. triphenylmethane dyes are synthetic compounds widely used in diverse industries. their removal from effluents is difficult, due to their high degree of structural complexity; hence, their high concentration in effluents cause a negative impact on the environment. in the present work, molecular docking was used to evaluate interactions between rgllcc1 or rpoxa 1b enzymes w ... | 2017 | 28866857 |
| simultaneously upgrading biogas and purifying biogas slurry using cocultivation of chlorella vulgaris and three different fungi under various mixed light wavelength and photoperiods. | in order to purify biogas slurry and biogas simultaneously, three different fungi, pleurotus geesteranus (p. geesteranus), ganoderma lucidum (g. lucidum), and pleurotus ostreatus (p. ostreatus) were pelletized with chlorella vulgaris (c. vulgaris). the results showed that the optimal light wavelength ratio for red:blue was 5:5 for these three different fungi-assisted c. vulgaris, resulting in higher specific growth rate as well as nutrient and co2 removal efficiency compared with other ratios. g ... | 2017 | 28618378 |
| advanced materials from fungal mycelium: fabrication and tuning of physical properties. | in this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi. mycelia from two types of edible, medicinal fungi, ganoderma lucidum and pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of ... | 2017 | 28117421 |
| evaluation of microwave-assisted and pressurized liquid extractions to obtain β-d-glucans from mushrooms. | microwave-assisted extraction (mae) and pressurized liquid extraction (ple) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from pleurotus ostreatus and ganoderma lucidum fruiting bodies. extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, rsm), using water as extraction solvent. total carbohydrate content of the obtained extracts and polysaccharide yields were the variable resp ... | 2017 | 27842810 |
| biosynthesis of nanoparticles of metals and metalloids by basidiomycetes. preparation of gold nanoparticles by using purified fungal phenol oxidases. | the work shows the ability of cultured basidiomycetes of different taxonomic groups-lentinus edodes, pleurotus ostreatus, ganoderma lucidum, and grifola frondosa-to recover gold, silver, selenium, and silicon, to elemental state with nanoparticles formation. it examines the effect of these metal and metalloid compounds on the parameters of growth and accumulation of biomass; the optimal cultivation conditions and concentrations of the studied ion-containing compounds for recovery of nanoparticle ... | 2017 | 27717965 |
| evaluation of pb (ii) biosorption utilizing sugarcane bagasse colonized by basidiomycetes. | the contamination of water resources by metallic ions is a serious risk to public health and the environment. therefore, a great emphasis has been given to alternative biosorption methods that are based on the retention of aqueous-solution pollutants; in the last decades, several agricultural residues have been explored as low-cost adsorbent. in this study, the ability of pb (ii) biosorption using sugarcane bagasse modified by different fungal species was evaluated. the presence of carbonyl, hyd ... | 2016 | 27063515 |
| computational analysis and low-scale constitutive expression of laccases synthetic genes gllcc1 from ganoderma lucidum and poxa 1b from pleurotus ostreatus in pichia pastoris. | lacasses are multicopper oxidases that can catalyze aromatic and non-aromatic compounds concomitantly with reduction of molecular oxygen to water. fungal laccases have generated a growing interest due to their biotechnological potential applications, such as lignocellulosic material delignification, biopulping and biobleaching, wastewater treatment, and transformation of toxic organic pollutants. in this work we selected fungal genes encoding for laccase enzymes gllcc1 in ganoderma lucidum and p ... | 2015 | 25611746 |
| a high throughput colorimetric assay of β-1,3-d-glucans by congo red dye. | mushroom strains contain complex nutritional biomolecules with a wide spectrum of therapeutic and prophylactic properties. among these compounds, β-d-glucans play an important role in immuno-modulating and anti-tumor activities. the present work involves a novel colorimetric assay method for β-1,3-d-glucans with a triple helix tertiary structure by using congo red. the specific interaction that occurs between congo red and β-1,3-d-glucan was detected by bathochromic shift from 488 to 516 nm (>20 ... | 2015 | 25555819 |
| the cancer preventive effects of edible mushrooms. | an increasing body of scientific literature suggests that dietary components may exert cancer preventive effects. tea, soy, cruciferous vegetables and other foods have been investigated for their cancer preventive potential. some non-edible mushrooms like reishi (ganoderma lucidum) have a history use, both alone and in conjunction with standard therapies, for the treatment of various diseases including cancer in some cultures. they have shown efficacy in a number of scientific studies. by compar ... | 2012 | 22583406 |
| mushroom polysaccharides and lipids synthesized in liquid agitated and static cultures. part i: screening various mushroom species. | the effect of four synthetic media containing glucose (initial concentration 30 g l(-1)) on mycelial growth, exopolysaccharides (eps) and cellular lipids production was examined in 11 mushroom species after 12 and 16 days of culture in static- and shake-flasks. fatty acid analysis of cellular lipids produced was also performed. agitation had a positive effect on biomass production, glucose consumption and lipid biosynthesis. media that favoured the production of biomass were not suitable for eps ... | 2012 | 22573010 |
| mushroom extracts decrease bone resorption and improve bone formation. | mushroom extracts have shown promising effects in the treatment of cancer and various chronic diseases. osteoporosis is considered one of the most widespread chronic diseases, for which currently available therapies show mixed results. in this research we investigated the in vitro effects of water extracts of the culinary-medicinal mushrooms trametes versicolor, grifola frondosa, lentinus edodes, and pleurotus ostreatus on a mc3t3-e1 mouse osteoblast-like cell line, primary rat osteoblasts, and ... | 2016 | 27649725 |
| kinetic characterization of lysine-specific metalloendopeptidases from grifola frondosa and pleurotus ostreatus fruiting bodies. | two zinc-metalloendopeptidases, gfmep (accession number p81054) and pomep (accession number p81055), from the fruiting bodies of two edible mushrooms, grifola frondosa and pleurotus ostreatus, respectively, specifically hydrolyze peptidyl-lysine bonds (-x-lys-) in polypeptides. to understand detailed substrate specificities and kinetic characters of these enzymes, we have synthesized various intramolecularly quenched fluorescent peptide substrates and determined their kinetic constants with thes ... | 1998 | 9644258 |
| amino acid sequences of metalloendopeptidases specific for acyl-lysine bonds from grifola frondosa and pleurotus ostreatus fruiting bodies. | the complete amino acid sequences of two lysine-specific zinc metalloendopeptidases (ec 3.4.24), grifola frondosa metalloendopeptidase (gfmep) and pleurotus ostreatus metalloendopeptidase (pomep), from the fruiting bodies of these two edible mushrooms have been established based on the sequence information of the peptides generated from the reduced and alkylated gfmep and pomep by proteolytic digestions using gfmep, trypsin, and other proteinases as well as by several chemical cleavages. from th ... | 1997 | 9374478 |
| effects of lentinus edodes, grifola frondosa and pleurotus ostreatus administration on cancer outbreak, and activities of macrophages and lymphocytes in mice treated with a carcinogen, n-butyl-n-butanolnitrosoamine. | icr mice were treated with a carcinogen, n-butyl-n'-butanolnitrosoamine bbn), every day for 8 consecutive weeks and the effects of oral administration of edible mushrooms on the induction of urinary bladder carcinoma and on the activities of macrophages and lymphocytes were studied. bladder carcinoma were found in all 10 mice (100%) treated with bbn alone, while we observed carcinoma only in 9 of 17 mice (52.9%), in 7 of 15 mice (46.7%) and 13 of 20 mice (65.0%) treated with lentinus edodes, gri ... | 1997 | 9130004 |
| bioremediation of long-term pcb-contaminated soil by white-rot fungi. | the objective of this work was to test the pcb-degrading abilities of two white-rot fungi, namely pleurotus ostreatus and irpex lacteus, in real contaminated soils with different chemical properties and autochthonous microflora. in addition to the efficiency in pcb removal, attention was given to other important parameters, such as changes in the toxicity and formation of pcb transformation products. moreover, structural shifts and dynamics of both bacterial and fungal communities were monitored ... | 2017 | 27894756 |
| downscaling the in vitro test of fungal bioremediation of polycyclic aromatic hydrocarbons: methodological approach. | the miniaturization and optimization of a white rot fungal bioremediation experiment is described in this paper. the optimized procedure allows determination of the degradation kinetics of anthracene. the miniaturized procedure requires only 2.5 ml of culture medium. the experiment is more precise, robust, and better controlled comparing it to classical tests in flasks. using this technique, different parts, i.e., the culture medium, the fungi, and the cotton seal, can be analyzed. a simple samp ... | 2016 | 26660875 |
| degradation of bunker c fuel oil by white-rot fungi in sawdust cultures suggests potential applications in bioremediation. | fungal lignocellulolytic enzymes are promising agents for oxidizing pollutants. this study investigated degradation of number 6 "bunker c" fuel oil compounds by the white-rot fungi irpex lacteus, trichaptum biforme, phlebia radiata, trametes versicolor, and pleurotus ostreatus (basidiomycota, agaricomycetes). averaging across all studied species, 98.1%, 48.6%, and 76.4% of the initial bunker c c10 alkane, c14 alkane, and phenanthrene, respectively were degraded after 180 days of fungal growth on ... | 2015 | 26111162 |
| biotransformation of fluoroquinolone antibiotics by ligninolytic fungi--metabolites, enzymes and residual antibacterial activity. | a group of white rot fungi (irpex lacteus, panus tigrinus, dichomitus squalens, trametes versicolor and pleurotus ostreatus) was investigated for the biodegradation of norfloxacin (nor), ofloxacin (of) and ciprofloxacin (cip). the selected fluoroquinolones were readily degraded almost completely by i. lacteus and t. versicolor within 10 and 14 d of incubation in liquid medium, respectively. the biodegradation products were identified by liquid chromatography-mass spectrometry. the analyses indic ... | 2015 | 25592459 |
| biotransformation of the antibiotic agent flumequine by ligninolytic fungi and residual antibacterial activity of the transformation mixtures. | flumequine, a fluoroquinolone antibiotic, is applied preferably in veterinary medicine, for stock breeding and treatment of aquacultures. formation of drug resistance is a matter of general concern when antibiotics such as flumquine occur in the environment. thus, biodegradation of flumequine in solution was investigated using five different ligninolytic fungi. irpex lacteus, dichomitus squalens, and trametes versicolor proved most efficient and transformed more than 90% of flumequine within 6 o ... | 2013 | 24261869 |
| differential proteomic analysis of the secretome of irpex lacteus and other white-rot fungi during wheat straw pretreatment. | identifying new high-performance enzymes or enzyme complexes to enhance biomass degradation is the key for the development of cost-effective processes for ethanol production. irpex lacteus is an efficient microorganism for wheat straw pretreatment, yielding easily hydrolysable products with high sugar content. thus, this fungus was selected to investigate the enzymatic system involved in lignocellulose decay, and its secretome was compared to those from phanerochaete chrysosporium and pleurotus ... | 2013 | 23937687 |
| incorporation of dietary fibre-rich oyster mushroom (pleurotus sajor-caju) powder improves postprandial glycaemic response by interfering with starch granule structure and starch digestibility of biscuit. | the purpose of this study was to determine the effects of pleurotus sajor-caju (psc) powder addition at 0, 4, 8 and 12% levels on the nutritional values, pasting properties, thermal characteristics, microstructure, in vitro starch digestibility, in vivo glycaemic index (gi) and sensorial properties of biscuits. elevated incorporation levels of psc powder increased the dietary fibre (df) content and reduced the pasting viscosities and starch gelatinisation enthalpy value of biscuits. the addition ... | 2017 | 28274444 |
| hypoglycemic and antidiabetic effect of pleurotus sajor-caju aqueous extract in normal and streptozotocin-induced diabetic rats. | pleurotus sajor-caju (psc) is an edible oyster mushroom featuring high nutritional values and pharmacological properties. objective. to investigate the hypoglycemic and antidiabetic effects of single and repeated oral administration of psc aqueous extract in normal and diabetic rats. | 2015 | 26682215 |
| important nutritional constituents, flavour components, antioxidant and antibacterial properties of pleurotus sajor-caju. | oyster mushroom (pleurotus sajor-caju) cultivated in the laboratory was studied for nutritional constituents, flavor components, antioxidant and antibacterial properties. nutritional constituents estimated per 100 g dry weight (d.w.) include protein (29.3 g), carbohydrate (62.97 g), crude fat (0.91 g), ash (6.82 g) and crude fiber (12.3 g). energy value of this mushroom was about 297.5 kcal/100 g d.w. major mineral components estimated include ca, fe, and mg with highest level of 505.0, 109.5 an ... | 2014 | 25114338 |
| over-expression of psgpd, a mushroom glyceraldehyde-3-phosphate dehydrogenase gene, enhances salt tolerance in rice plants. | transgenic potatoes expressing glyceraldehyde-3-phosphate dehydrogenase (gpd), isolated from the oyster mushroom, pleurotus sajor-caju, had increased tolerance to salt stress (jeong et al. biochem biophys res commun 278:192-196, 2000). to examine the physiological mechanisms enhancing salt tolerance in gpd-transgenic rice plants, the salt tolerance of five gpd transgenic rice lines (t1-t5) derived from dongjin rice cultivar were evaluated in a fixed 150 mm saline environment in comparison to two ... | 2014 | 24737077 |
| toxicity of organic and inorganic nanoparticles to four species of white-rot fungi. | the rapid development of nanoparticles (np) for industrial applications and large-volume manufacturing, with its subsequent release into the environment, raised the need to understand and characterize the potential effects of np to biota. accordingly, this work aimed to assess sublethal effects of five np to the white-rot fungi species trametes versicolor, lentinus sajor caju, pleurotus ostreatus, and phanerochaete chrysosporium. each species was exposed to serial dilutions of the following np: ... | 2013 | 23665417 |
| antioxidant properties of different edible mushroom species and increased bioconversion efficiency of pleurotus eryngii using locally available casing materials. | total phenolics, radical scavenging activity (rsa) on dpph, ascorbic acid content and chelating activity on fe(2+) of pleurotus citrinopileatus, pleurotus djamor, pleurotus eryngii, pleurotus flabellatus, pleurotus florida, pleurotus ostreatus, pleurotus sajor-caju and hypsizygus ulmarius have been evaluated. the assayed mushrooms contained 3.94-21.67 mg tae of phenolics, 13.63-69.67% dpph scavenging activity, 3.76-6.76 mg ascorbic acid and 60.25-82.7% chelating activity. principal component ana ... | 2013 | 23411281 |
| [the commercially cultivated edible oyster mushrooms pleurotus sajor-caju and p. pulmonarius are two separate species, similar in morphology but reproductively isolated]. | two closely related commercially cultivated oyster mushroom species, pleurotus pulmonarius and p. sajor-caju have been differentiated by traditional mating experiments as well as analysis of the variable its and igs sequences of the ribosomal gene cluster. molecular analysis of the variable its and igs regions has allowed neither reliable differentiation between the morphologically similar species p. pulmonarius and p. sajor-caju nor confirmation of species identity of the p. sajor-caju strains ... | 2012 | 23297481 |
| effect of incorporation of mushroom (pleurotus sajor-caju) powder on quality characteristics of papad (indian snack food). | papad is familiarly known as an indian food adjunct or snack food. in this study, attempts have been made to increase the protein, minerals and crude fibre content of the papad with incorporation of pleurotus sajor-caju powder to develop a novel snack food. mushroom powder was added in different proportions with other ingredients of the papad and the prepared papad was subjected to physicochemical and sensorial analyses. mushrooms pretreated with 1% potassium metabisulphite followed by whey trea ... | 2012 | 22574961 |
| yield performances and changes in enzyme activities of pleurotus spp. (p. ostreatus and p. sajor-caju) cultivated on different agricultural wastes. | pleurotus ostreatus and pleurotus sajor-caju were studied for their ability to produce laccase and carboxymethylcellulase (cmcase) enzymes on different agricultural wastes under solid state fermentation. the spawns of p. ostreatus and p. sajor-caju were inoculated on different agricultural wastes including viticulture wastes, wheat straw, paddy straw, sesame straw, sawdust as well as the mixtures of these wastes with wheat bran. the carbon and nitrogen contents of substrates containing bran were ... | 2010 | 20056410 |
| effect of substrate quantity and shelf position on yield of oyster mushroom (pleurotus sajor caju). | an experiment was conducted in harare (zimbabwe) in 2004 to investigate the effect of substrate (wheat straw) quantity and shelf position on yield of oyster mushroom (pleurotus sajor-caju) using plastic tray culture. the experiment was laid out as a 2-way factorial in a randomized block design with five substrate quantities (2, 4, 6, 8 and 10 kg of wheat straw per tray) and two shelf positions (0.5 and 1.2 m above ground). mushroom yield increased with an increased substrate quantity of up to 6 ... | 2007 | 19090170 |
| the effect of pleurotus spp. fungi on chemical composition and in vitro digestibility of rice straw. | this study was carried out to test the potentially of using rice straw substrate for the cultivation of four pleurotus species including pleurotus florida, pleurotus djamor, pleurotus sajor-caju and pleurotus ostreatus and the effect of these species on the chemical composition, cell wall degradation and digestibility of rice straw. rice straw soaked in water for 24 h and then it was pasteurized at 100 degrees c for 6 h. rice straw was inoculated with spawns of four pleurotus fungi (pleurotus fl ... | 2007 | 19070114 |
| the use of the valuable oyster mushroom, pleurotus sajor-caju, for conversion of waste materials produced from seaweed and brewing industries: preliminary investigations. | | 2003 | 12691496 |
| biotransformation of banana waste into protein by pleurotus sajor-caju. | pseudostems and leaves from banana waste were used for biotransformation into protein by using p. sajor-caju, an oyster mushroom. treatment of formalin (500 ppm) + carbendazim (12.5 ppm) of these substrates was found to favour relatively high percentage biological efficiency (be) of p. sajor-caju.. steam sterilization also exhibited comparable yield performance by p. sajor-caju. fruiting bodies harvested from all the treatments had relatively higher protein contents. the spent substrate (steam s ... | 2000 | 12557923 |
| oyster mushroom cultivation with rice and wheat straw. | cultivation of the oyster mushroom, pleurotus sajor-caju, on rice and wheat straw without nutrient supplementation was investigated. the effects of straw size reduction method and particle size, spawn inoculation level, and type of substrate (rice straw versus wheat straw) on mushroom yield, biological efficiency, bioconversion efficiency, and substrate degradation were determined. two size reduction methods, grinding and chopping, were compared. the ground straw yielded higher mushroom growth r ... | 2002 | 11991077 |
| potential enrichment of medicinal mushrooms with selenium to obtain new dietary supplements. | the purpose of this study was to resolve the question of whether various selenium concentrations affect the ability of eight species, members of five genera, to produce mycelium biomass and absorb this trace element during submerged cultivation in na2seo3-enriched medium. the obtained results demonstrated the ability of mycelia of all of the tested species to absorb selenium at analyzed concentrations. thus, selenium content ranged from 1.4 µg g-1 (pleurotus eryngii) to 20.3 µg g-1 (pleurotus os ... | 2013 | 24266370 |
| promising cellulolytic fungi isolates for rice straw degradation. | the objective of this study was to evaluate the potential of eight fungal isolates obtained from soils in rice crops for straw degradation in situ. from the initial eight isolates, pleurotus ostreatus t1.1 and penicillium sp. hc1 were selected for further characterization based on qualitative cellulolytic enzyme production and capacity to use rice straw as a sole carbon source. subsequently, cellulolytic, xylanolytic, and lignolytic (pleurotus ostreatus) activity on carboxymethyl cellulose, oat ... | 2017 | 28865071 |
| the effects of different substrates on the growth, yield, and nutritional composition of two oyster mushrooms (pleurotus ostreatus and pleurotus cystidiosus). | the study was conducted to compare the effects of different agro-wastes on the growth, yield, and nutritional composition of oyster mushrooms pleurotus ostreatus (po) and pleurotus cystidiosus (pc). seven substrate formulas including sawdust (sd), corncob (cc), sugarcane bagasse (sb) alone and in combination of 80 : 20, 50 : 50 ratio between sd and cc, sd and sb were investigated. the results indicated that different substrate formulas gave a significant difference in total colonization period, ... | 2015 | 26839502 |
| the effects of temperature and nutritional conditions on mycelium growth of two oyster mushrooms (pleurotus ostreatus and pleurotus cystidiosus). | the influences of temperature and nutritional conditions on the mycelium growth of oyster mushroom pleurotus ostreatus (po) and pleurotus cystidiosus (pc) were investigated in laboratory experiment during the summer season of 2014. the results of the experiment indicated that potato dextrose agar (pda) and yam dextrose agar (yda) were the most suitable media for the mycelium growth of oyster mushroom po while four media (pda, yda, sweet potato dextrose agar, and malt extract agar medium) were no ... | 2015 | 25892910 |
| hypoglycaemic activity of culinary pleurotus ostreatus and p. cystidiosus mushrooms in healthy volunteers and type 2 diabetic patients on diet control and the possible mechanisms of action. | this study determined the oral hypoglycaemic effect of suspensions of freeze dried and powdered (sfdp) pleurotus ostreatus (p.o) and pleurotus cystidiosus (p.c), using healthy human volunteers and type 2 diabetic patients on diet control at a dose of 50 mg/kg/body weight, followed by a glucose load. the possible hypoglycaemic mechanisms were evaluated using rats, by examining intestinal glucose absorption and serum levels of insulin, glucokinase (gk) and glycogen synthase kinase (gsk). the p.o a ... | 2015 | 25382404 |
| characteristic odorants from bailingu oyster mushroom (pleurotus eryngii var. tuoliensis) and summer oyster mushroom (pleurotus cystidiosus). | in this study, the characteristic odorants of the volatile oils from pleurotus species (p. eryngii var. tuoliensis and p. cystidiosus) were extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (gc-ms), gas chromatography-olfactometry (gc-o), and aroma extract dilution analysis (aeda). a total of 52 and 54 components (p. eryngii var. tuoliensis and p. cystidiosus, respectively) were identified, representing about 98.8% and 85.1% of the volatile oils, respectively. t ... | 2014 | 24919476 |
| high-performance of agaricus blazei fungus for the biological pretreatment of elephant grass. | biological pre-treatment seems to be promising being an eco-friendly process, with no inhibitor generated during the process. the potential for elephant grass pre-treatment with white degradation fungi pleurotus ostreatus, agaricus blazei, lentinula edodes, pleurotus citrinopileatus, and pleurotus djamor, in isolated or mixed cultures of these strains, was evaluated. the highest activities of enzymes involved in the degradation of lignocellulosic biomass (laccases, endoglucanases, xylanases, and ... | 2017 | 28726354 |
| rapid strain classification and taxa delimitation within the edible mushroom genus pleurotus through the use of diffuse reflectance infrared fourier transform (drift) spectroscopy. | fourier transform infrared (ft-ir) spectroscopy has been successfully applied for the identification of bacteria and yeasts, but only to a limited extent for discriminating specific groups of filamentous fungi. in the frame of this study, 73 strains - from different associated hosts/substrates and geographic regions - representing 16 taxa of the edible mushroom genus pleurotus (basidiomycota, agaricales) were examined through the use of diffuse reflectance infrared fourier transform (drift) spec ... | 2012 | 22658316 |
| isolation and characterization of a novel ribonuclease from the pink oyster mushroom pleurotus djamor. | a 15-kda rnase was purified from pleurotus djamor using ion exchange chromatography and gel filtration. its n-terminal amino acid sequence was different from previously reported rnase sequences of mushrooms belonging to the genus of pleurotus and other genera. the rnase exhibited maximal rnase activity at ph 4.6 and 60 degrees c. its activity toward polyhomoribonucleotides was poly(u) > poly(c) > poly(a) > poly(g). it inhibited proliferation of hepatoma cells and breast cancer cells. the ranking ... | 2010 | 20647680 |
| evaluation of two wild types of pleurotus ostreatus (mcc07 and mcc20) isolated from nature for their ability to decolorize benazol black zn textile dye in comparison to some commercial types of white rot fungi: pleurotus ostreatus, pleurotus djamor, and pleurotus citrinopileatus. | biological decolorization of benazol black zn, a reactive azo-type textile dyestuff, was comparatively studied using 3 different commercial-type white rot fungi strains (pleurotus ostreatus, pleurotus cornucopiae var. citrinopileatus, pleurotus djamor, and 2 wild types of p. ostreatus (mcc07 and mcc20) isolated from the nature. the initial dye concentrations in the medium were 500 and 1000 mg.l-1. all the organisms studied decolorized benazol black zn to varying degrees. at low dye concentration ... | 2008 | 18449221 |
| [studies on genus pleurotus. viii. interaction between mycelial growth and yield]. | this project studies the relationship between mycelial growth rate and production of basidiomata of 19 pleurotus strains. firstly, monosporic cultures were isolated of five strains from the following species: pleurotus djamor (3), pleurotus ostreatus (1) and pleurotus pulmonarius (1). these were self-crossed in order to obtain 25 infraspecific dikaryons from which their mycelial growth rate was estimated. the parent strains and the 14 fastest growing crosses were cultivated in the pilot plant on ... | 1997 | 15538821 |
| the genetic structure and diversity of the a and b mating-type genes from the tropical oyster mushroom, pleurotus djamor. | in most heterothallic mushroom species, inbreeding is avoided by an incompatibility system determined by two loci each with multiple alleles (the a and b mating-type loci). in this study we investigated the genetic structure of the mating-type loci in the tropical oyster mushroom pleurotus djamor using both positional cloning and degenerate pcr methods. dna sequences from genomic regions cosegregating with the mating-type loci of p. djamor revealed homeodomain transcription factors (a) and phero ... | 2004 | 15219565 |
| bioremediation of aflatoxin b1-contaminated maize by king oyster mushroom (pleurotus eryngii). | aflatoxin b1 (afb1) is the most harmful mycotoxin that occurs as natural contaminant of agricultural commodities, particularly maize. practical solutions for detoxification of contaminated staples and reduction of agricultural wastes are scarce. we investigated the capability of the white-rot and edible fungus plerotus eryngii (king oyster mushroom) to degrade afb1 both in vitro and in a laboratory-scale mushroom cultivation, using a substrate similar to that routinely used in mushroom farms. in ... | 2017 | 28771640 |
| investigating the effect of tissue size on mycelial growth of seven mushroom species by using a novel device for precise tissue isolation. | tissue isolation from mushrooms is frequently practiced by both researchers and growers to isolate new and improved strains. in the present study, we designed a simple and convenient device for precise tissue isolation and therefore investigated the effect of tissue size on mycelial growth of seven mushroom species. the developed device consists of a cutting needle and a transfer needle. the cutting needle was used to obtain circular tissue plugs having a height up to 3 mm and variable diameters ... | 2016 | 27784952 |
| effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms. | influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (agaricus bisporus, lentinula edodes, pleurotus ostreatus, and pleurotus eryngii) was studied. proximate composition was affected by the cooking method and the mushrooms species. frying induced more severe losses in protein, ash, and carbohydrates content but increased the fat and energy. boiling improved the total glucans content by enhanci ... | 2017 | 27760471 |
| the anti-adipogenic activity of a new cultivar, pleurotus eryngii var. ferulae 'beesan no. 2', through down-regulation of ppar γ and c/ebp α in 3t3-l1 cells. | adipogenesis is one of the cellular processes and a highly controlled program. nowadays, inhibition of adipogenesis has received attention as an effective way to regulate obesity. in the current study, we investigated the inhibition effect of a chloroform extract of pleurotus eryngii var. ferulae 'beesan no. 2' (cebt) on adipogenesis in 3t3-l1 murine preadipocytes. pleurotus eryngii var. ferulae is one of many varieties of king oyster mushroom and has been reported to have various biological act ... | 2016 | 27470278 |
| toughening and its association with the postharvest quality of king oyster mushroom (pleurotus eryngii) stored at low temperature. | toughening-induced textural decay easily occurs in stored mushrooms. the objective of this study was to investigate the textural alteration caused by toughening in relation to other quality attributes of king oyster mushroom (pleurotus eryngii). fresh king oyster mushrooms, packed in low-density polyethylene bags, were stored at different low temperatures for 18 days to measure textural and other quality attributes. it was found that toughening occurred twice during the entire storage time. high ... | 2016 | 26593593 |
| improving the ph-stability of versatile peroxidase by comparative structural analysis with a naturally-stable manganese peroxidase. | versatile peroxidase (vp) from the white-rot fungus pleurotus eryngii is a high redox potential peroxidase of biotechnological interest able to oxidize a wide range of recalcitrant substrates including lignin, phenolic and non-phenolic aromatic compounds and dyes. however, the relatively low stability towards ph of this and other fungal peroxidases is a drawback for their industrial application. a strategy based on the comparative analysis of the crystal structures of vp and the highly ph-stable ... | 2015 | 26496708 |
| influence of customized cooking methods on the phenolic contents and antioxidant activities of selected species of oyster mushrooms (pleurotus spp.). | nutritional value of cooked food has been considered to be lower compared to the fresh produce. however, many reports showed that processed fruits and vegetables including mushrooms may retain antioxidant activity. pleurotus spp. as one of the edible mushroom are in great demand globally and become one of the most popular mushrooms grown worldwide with 25-fold increase in production from 1960-2009. the effects of three different cooking methods (boiling, microwave and pressure cooking) on the an ... | 2015 | 25892809 |