Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| methanol extract of the oyster mushroom, pleurotus florida, inhibits inflammation and platelet aggregation. | the antiinflammatory and platelet aggregation inhibiting activities of the methanol extract of pleurotus florida eger, an edible and commercially grown mushroom, were investigated. the extract showed significant activity in ameliorating acute inflammation induced by carrageenan and chronic inflammation by formalin at 500 and 1000 mg/kg body weight. the effect was comparable to the standard reference drug, diclofenac. the extract also showed significant platelet aggregation inhibiting activity of ... | 2004 | 14750200 |
| production and induction of manganese peroxidase isozymes in a white-rot fungus pleurotus ostreatus. | the production of mnp by pleurotus ostreatus in different liquid cultures was investigated. the highest level of activity was observed after 8 days of culture in peptone-glucose-yeast extract medium (pgy), whereas maximal activity was achieved after 30 days in glucose-yeast extract medium (gy). mnp was purified to homogeneity from pgy (designated mnp-pgy) and gy (mnp-gy). the isoelectric points of mnp-pgy and mnp-gy were 3.77 and 4.06, respectively. the molecular mass of both enzymes was 42 kda. ... | 2004 | 14767623 |
| [aflp fingerprinting map analysis of pleurotus ostreatus]. | aflp analysis was carried out with 14 pleurotus ostreatus strains from different areas. optimal conditions of the aflp fingerprinting analysis for p. ostreatus were first tested and the results showed that the primer pairs e-3/m-3 especially e-agc/m-cat and e-agc/m-acc could give more amplified dna fragments than others like e-2/m-1 or e-2/m-3. from the fingerprinting map of the primer pair e-agc/m-cat, 184 clear and stable dna bands were observed, including 101 polymorphous bands that are accou ... | 2003 | 14986432 |
| fungal upgrading of wheat straw for straw-thermoplastics production. | combining biologic pretreatment with storage is an innovative approach for improving feedstock characteristics and cost, but the magnitude of responses of such systems to upsets is unknown. unsterile wheat straw stems were upgraded for 12 wk with pleurotus ostreatus at constant temperature to estimate the variation in final compositions with variations in initial moisture and inoculum. degradation rates and conversions increased with both moisture and inoculum. a regression analysis indicated th ... | 2004 | 15054197 |
| decolorization of orange g by pleurotus ostreatus monokaryotic isolates with different laccase activity. | the effect of enhanced laccase (lac) activity (obtained after copper addition to cultivation media) on decolorization of azo dye orange g in two basidiospore-derived monokaryotic isolates of pleurotus ostreatus was determined. the high lac-producing isolate efficiently decolorized orange g. the low-producing isolate showed only poor decolorization ability during cultivation in liquid medium and no decolorization on agar plates containing orange g after a 25-d growth. a substantial enhancement of ... | 2003 | 15058191 |
| pleurotolysin, a novel sphingomyelin-specific two-component cytolysin from the edible mushroom pleurotus ostreatus, assembles into a transmembrane pore complex. | self-assembling, pore-forming cytolysins are illustrative molecules for the study of the assembly and membrane insertion of transmembrane pores. here we purified pleurotolysin, a novel sphingomyelin-specific two-component cytolysin from the basidiocarps of pleurotus ostreatus and studied the pore-forming properties of the cytolysin. pleurotolysin consisted of non-associated a (17 kda) and b (59 kda) components, which cooperatively caused leakage of potassium ions from human erythrocytes and swel ... | 2004 | 15084605 |
| biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and pure cultures of imperfect and lignolitic fungi. | the biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and seven fungi species, including imperfect strains and higher level lignolitic species, is compared in a 90-day laboratory experiment using a natural, not-fertilized soil contaminated with 10% crude oil. the natural microbial soil assemblage isolated from an urban forest area was unable to significantly degrade crude oil, whereas pure fungi cultures effectively reduced the residues by 26-35% in 90 days. normal ... | 1996 | 15093496 |
| fungal laccase-catalyzed degradation of hydroxy polychlorinated biphenyls. | hydroxy polychlorinated biphenyls (hydroxy pcbs) are toxic metabolites of pcbs. their toxicity such as strong endocrine disruption demands effective remediation methods. laccases from trametes versicolor and pleurotus ostreatus were tested to degrade hydroxy pcbs. optimum phs for both enzymes were around 4.0. laccase from t. versicolor degrades hydroxy pcbs more rapidly than that from p. ostreatus. the enzymatic activities remained little changes in up to 10% organic solvents, but decreased rapi ... | 2004 | 15109876 |
| sequence polymorphism and molecular characterization of laccase genes of the conifer pathogen heterobasidion annosum. | the oxidizing enzyme laccase produced by many fungi is generally considered to be active in the biodegradation of lignin, a major plant cell wall component highly resistant to microbial attack. the enzyme is secreted at high levels by the p-type of the highly aggressive pathogen heterobasidion annosum, but at much lower levels by the s-type which correlated with their varying wood decay capability. to investigate the evolutionary relationship between laccase genes of the different h. annosum typ ... | 2004 | 15119350 |
| key elements for protein foldability revealed by a combinatorial approach among similarly folded but distantly related proteins. | we have determined the key regions for protein foldability by creating multiple crossover libraries from two proteins that share similar fold but have low sequence identity and differ significantly in stability. one protein is the propeptide of a serine protease, subtilisin bpn', and the other is pleurotus ostreatus proteinase a inhibitor 1 (poia1). the propeptide has a compact structure when complexed with subtilisin but is unstructured when isolated, whereas poia1 takes a stable structure. we ... | 2004 | 15157092 |
| tolaasins a--e, five new lipodepsipeptides produced by pseudomonas tolaasii. | pseudomonas tolaasii, the causal organism of brown blotch disease of agaricus bisporus and of the yellowing of pleurotus ostreatus, was shown to produce in culture tolaasin i (1), tolaasin ii (2), and five other minor metabolites, tolaasins a, b, c, d, and e (3-7). these compounds were demonstrated to be important in the development of the disease symptoms. this paper reports on the structural elucidation, based essentially on nmr studies and ms spectra, and biological activity of the above lipo ... | 2004 | 15165142 |
| a new ribonuclease from the black oyster mushroom pleurotus ostreatus. | a ribonuclease that is co-specific for poly c and poly u has been isolated from the fruiting bodies of the black oyster mushroom. the enzyme possesses a molecular mass of 14 kda, and is unadsorbed on deae-cellulose and adsorbed on affi-gel blue gel and cm-cellulose. a ph of 7 is required for the enzyme to exhibit maximal activity. the activity of the enzyme does not vary appreciably over the temperature range 30-60 degrees c, but drops when the temperature is reduced to 20 degrees c or raised to ... | 2004 | 15165725 |
| isolation of a new ribonuclease from fruiting bodies of the silver plate mushroom clitocybe maxima. | a ribonuclease, with an n-terminal sequence exhibiting some homology to ribonuclease from pleurotus ostreatus (family pleurotaceae), has been purified from fruiting bodies of the silver plate mushroom clitocybe maxima (family tricholomataceae). however, there is little resemblance between the n-terminal sequences of ribonucleases from various pleurotus species, and a lesser extent of resemblance between ribonucleases from c. maxima and pleurotus tuber-regium. no structural relationship exists be ... | 2004 | 15203239 |
| [ultrastructure of the cell wall in vegetative mycelia of agaricus bisporus (lange) imbach]. | a comparative description of the cell wall ultrastructure was made for vegetative mycelia in agaricus bisporus, other species of agaricus, and pleurotus ostreatus. apart from well known ultrastructural features of cell walls of the examined strains, inclusions of different kinds were observed. the cell wall thickness varied in different agaricus strains. maximum differences between the examined strains were 1.4 times. the cell wall thickness in p. ostreatus strains was by 3 to 4 times less than ... | 2004 | 15214164 |
| the genetic structure and diversity of the a and b mating-type genes from the tropical oyster mushroom, pleurotus djamor. | in most heterothallic mushroom species, inbreeding is avoided by an incompatibility system determined by two loci each with multiple alleles (the a and b mating-type loci). in this study we investigated the genetic structure of the mating-type loci in the tropical oyster mushroom pleurotus djamor using both positional cloning and degenerate pcr methods. dna sequences from genomic regions cosegregating with the mating-type loci of p. djamor revealed homeodomain transcription factors (a) and phero ... | 2004 | 15219565 |
| improving the yield and quality of dna isolated from white-rot fungi. | a new simple method used to eliminate polysaccharides that cause problems during dna isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (ctab) as wash buffer and followed by centrifugation. variation in the dna yield and quality was ascertained using precipitating agents, detergents and cell-wall-hydrolyzing chitinase. considerable amount of exopolysaccharides from fungal biomass was removed with the use of 1% ctab wash buffer followed by centri ... | 2004 | 15227780 |
| cloning, expression, and pore-forming properties of mature and precursor forms of pleurotolysin, a sphingomyelin-specific two-component cytolysin from the edible mushroom pleurotus ostreatus. | pleurotolysin, a sphingomyelin-specific cytolysin consisting of a (17 kda) and b (59 kda) components from the basidiomycete pleurotus ostreatus, assembles into a transmembrane pore complex. here, we cloned complementary and genomic dnas encoding pleurotolysin, and studied pore-forming properties of recombinant proteins. the genomic regions encoding pleurotolysin a and b contained two and eight introns, respectively, and putative promoter sequences. the complementary dna (cdna) for pleurotolysin ... | 2004 | 15245918 |
| the mannitol cycle in pleurotus ostreatus (florida). | in mushroom, presence of the mannitol cycle has not been reported so far although the polyol is supposed to be generated by the reduction of fructose by mannitol dehydrogenase. this study submits evidence for the presence of the mannitol cycle in pleurotus ostreatus. the key enzyme of the cycle, mannitol-1-phosphate dehydrogenase (m1pdh), was present appreciably in all the developmental stages of the mushroom. however, the enzyme level dropped significantly at the onset of sporulation. the prese ... | 2004 | 15251212 |
| degradation of acrylic copolymers by white-rot fungi. | various water-soluble homopolymers and copolymers of acrylamide (aam) and acrylic acid (aa) which contained phenolic sites, such as guaiacol, lignin sulfonate (ls) and 3,4-dihydroxybenzoic acid (3,4-dhba), were tested with regard to their degradability by white-rot fungi. compared with phanerochaete chrysosporium, pleurotus ostreatus caused a significantly higher decrease in the average molecular weight ( mw) of most of the copolymers and the homopolymer under the applied culture conditions. how ... | 2004 | 15257422 |
| solid-state fermentation of lignocellulosic plant residues from brassica napus by pleurotus ostreatus. | solid-state fermentation (ssf) of inedible parts of rapeseed was carried out using a white-rot fungus, pleurotus ostreatus, to degrade lignocellulosic material for mycelial-single cell protein (scp) production. this ssf system has the potential to be adapted to a controlled ecological life support system in space travel owing to the lack of storage space. the system for converting lignocellulosic material to scp by p. ostreatus is simple; it can be carried out in a compact reactor. the fungal ve ... | 1999 | 15304774 |
| can mushrooms fix atmospheric nitrogen? | it is generally reported that fungi like pleurotus spp. can fix nitrogen (n2). the way they do it is still not clear. the present study hypothesized that only associations of fungi and diazotrophs can fix n2. this was tested in vitro. pleurotus ostreatus was inoculated with a bradyrhizobial strain nodulating soybean and p. ostreatus with no inoculation was maintained as a control. at maximum mycelial colonization by the bradyrhizobial strain and biofilm formation, the cultures were subjected to ... | 2004 | 15381850 |
| ostreolysin, a pore-forming protein from the oyster mushroom, interacts specifically with membrane cholesterol-rich lipid domains. | ostreolysin, a 15 kda pore-forming protein from the edible oyster mushroom (pleurotus ostreatus), is lytic to membranes containing both cholesterol and sphingomyelin. its cytotoxicity to chinese hamster ovary cells correlates with their cholesterol contents and with the occurrence of ostreolysin in the cells detergent resistant membranes. moreover, ostreolysin binds to supported monolayers and efficiently permeabilizes sonicated lipid vesicles, only if cholesterol is combined with either sphingo ... | 2004 | 15388337 |
| cloning and developmental expression of a metzincin family metalloprotease cdna from oyster mushroom pleurotus ostreatus. | a cdna clone, pomtp, encoding a putative metzincin family metalloprotease was isolated from the expressed sequence tags of a basidiomycete pleurotus ostreatus. the 5'-end sequence of pomtp was determined by the 5'-race method. full-length cdna sequence (1140 bp) of pomtp contained a 870 bp open reading frame encoding a protein product of 290 amino acids in addition to a 99 bp of 5'-untranslated sequence and a 171 bp of 3'-untranslated sequence with a poly(a) tail. the deduced amino-acid sequence ... | 2004 | 15451101 |
| [treatment of coloured industrial effluents with pleurotus spp]. | the decolouration of fermentation residues (vinasse) and liquid extract of coffee pulp by the mushroom pleurotus ostreatus was studied in addition to laccase activity. the fungus was inhibited in both residues when they remained undiluted. in submerged cultivation on wastewaters a good production of biomass (14.8 g/l for vinasse and 5.4 g/l for extract of coffee pulp) and also laccase activity (14.1 u/ml for vinasse and 3.0 u/ml for extract of coffee pulp) up to the 10 days of fermentation was o ... | 2003 | 15456356 |
| direct oxidation of polymeric substrates by multifunctional manganese peroxidase isoenzyme from pleurotus ostreatus without redox mediators. | vps (versatile peroxidases) sharing the functions of lip (lignin peroxidase) and mnp (manganese peroxidase) have been described in basidiomycetous fungi pleurotus and bjerkandera. despite the importance of this enzyme in polymer degradation, its reactivity with polymeric substrates remains poorly understood. in the present study, we first report that, unlike lip, vp from pleurotus ostreatus directly oxidized two polymeric substrates, bovine pancreatic rnase and poly r-478, through a long-range e ... | 2005 | 15461584 |
| [interspecific genetic variability of the oyster mushroom pleurotus ostreatus as revealed by allozyme gene analysis]. | allozyme variation in natural populations of basidiomycetes fungus pleurotus ostreatus (88 individuals) from three regions of central russia was studied. the species was shown to have 92.86% of polymorphic allozyme loci and expected heterozygosity he = 0.49. the mean number of alleles per locus was 3.5. the genetic differences among populations were supported by f-statistics (fst = 0.750). the low level of inbreeding (fis = 0.018) suggests that the p. ostreatus populations are panmictic, and the ... | 2004 | 15523846 |
| manganese peroxidase of agaricus bisporus: grain bran-promoted production and gene characterization. | the main manganese peroxidase (mnp) isoenzyme of agaricus bisporus atcc 62459 produced in lignocellulose-containing cultures was isolated, cloned and sequenced. in liquid medium, where mnp was previously detected only in trace amounts, the production of mnp was enhanced by rye and wheat bran supplements. the pi (3.25) and n-terminal amino acid sequence (25 aa) of the enzyme from bran-containing cultures were identical to those reported from compost-isolated mnp1. mnp1 is a 328-aa long polypeptid ... | 2005 | 15538559 |
| [studies on genus pleurotus. viii. interaction between mycelial growth and yield]. | this project studies the relationship between mycelial growth rate and production of basidiomata of 19 pleurotus strains. firstly, monosporic cultures were isolated of five strains from the following species: pleurotus djamor (3), pleurotus ostreatus (1) and pleurotus pulmonarius (1). these were self-crossed in order to obtain 25 infraspecific dikaryons from which their mycelial growth rate was estimated. the parent strains and the 14 fastest growing crosses were cultivated in the pilot plant on ... | 1997 | 15538821 |
| analysis of the carbohydrate binding specificity of the mushroom pleurotus ostreatus lectin by surface plasmon resonance. | the sugar binding specificity of the mushroom pleurotus ostreatus lectin (pol) was analyzed by surface plasmon resonance. the lectin was immobilized to a sensor chip, and asialo-bovine submaxillary mucin (asialo-bsm), one of the most potent inhibitors in the hemagglutination inhibition assay, tightly bound to the lectin. the binding specificity of various mono- or oligosaccharides to the lectin was evaluated by the coinjection method. the dissociation of asialo-bsm was promoted by injection of s ... | 2005 | 15582562 |
| mass spectrometry and database search in the analysis of proteins from the fungus pleurotus ostreatus. | tandem mass spectrometry is a method of choice for rapid analysis in proteomics. identification and characterization of proteins from organisms with sequenced genomes is today a routine procedure as will be identification of proteins from organisms with unsequenced genomes with new developing tools. here, we report the use of isotopic labeling with electrospray ionisation (esi)-tandem mass spectrometry for de novo sequencing in combination with database search taking advantage of different progr ... | 2005 | 15602771 |
| mn(2+) is dispensable for the production of active mnp2 by pleurotus ostreatus. | the regulation mechanism for expression of versatile peroxidase mnp2 by the basidiomycete fungus pleurotus ostreatus was examined using chemically defined synthetic media. expression of mnp2 was down-regulated at the transcription level by nutrient nitrogen, e.g., nh(4)(+), arginine or urea. as is often the case with other fungal manganese peroxidases, active mnp2 was not detected when mn(2+) was omitted from the culture, while mnp2 transcription was barely affected by mn(2+). however, mn(2+) ca ... | 2005 | 15649426 |
| biodegradation of lindane by pleurotus ostreatus via central composite design. | the degradation of lindane was studied in liquid-agitated cultures using a commercial strain of the fungus pleurotus ostreatus as the biodegrading organism. the biodegradation was accomplished with the action of extracellular oxidative enzymes, produced by the fungus to decompose woody substrates. enzyme activities of manganese peroxidase and laccase were measured in a liquid mineral medium. an orthogonal central composite design of experiments was used to construct second-order response surface ... | 2005 | 15661282 |
| characterization of phytase activity from cultivated edible mushrooms and their production substrates. | phytase is used commercially to maximize phytic acid degradation and to decrease phosphorus levels in poultry and swine manure. to determine phytase content in edible mushrooms, basidiomata of agaricus bisporus and three specialty mushrooms (grifola frondosa, lentinula edodes, and pleurotus cornucopiae) and spent mushroom substrate (sms) were surveyed. enzyme activity ranged from 0.046 to 0.074 unit/g of tissue for four a. bisporus types (closed and open whites and closed and open browns) grown ... | 2004 | 15675798 |
| complete nucleotide sequence and genome organization of a dsrna partitivirus infecting pleurotus ostreatus. | the nucleotide sequences of the genomic dsrna mycovirus infecting pleurotus ostreatus (p. ostreatus virus 1; pov1) were determined and compared to the sequences of the other mycoviruses belonging to partitiviruses and totivirues. pov1 dsrna-1 and dsrna-2 had genomes of 2296 and 2223 nucleotides, respectively. the purified virus preparations contained isometric particles of 28-30 nm in diameter, and also the same two dsrnas were isolated from purified virus preparations. the sequences of pov1 dsr ... | 2005 | 15681061 |
| determination of biodegradation products from sulfonated dyes by pleurotus ostreatususing capillary electrophoresis coupled with mass spectrometry. | microbial treatment of environmental pollutants including dyes with white rot fungi has received wide attention as a potential alternative for conventional methods in wastewater treatment. the degradation products from dyes and mechanism underlying fungal degradation of dyes is desirable to be understood. capillary electrophoresis coupled with mass spectrometry (ce-ms) was used in this study to determine biodegradation products of 4-[(4-hydroxyphenyl)azo]-benzenesulfonic acid, sodium salt (4haba ... | 2005 | 15685423 |
| a new subfamily of fungal subtilases: structural and functional analysis of a pleurotus ostreatus member. | pleurotus ostreatus produces several extracellular proteases which are believed to be involved in the regulation of the ligninolytic activities of this fungus. recently, purification and characterization of the most abundant p. ostreatus extracellular protease (posl) have been reported. the sequence of the posl gene and of the corresponding cdna has been determined, allowing the identification of its pre- and pro-sequences. a mature protein sequence has been verified by mass spectrometry mapping ... | 2005 | 15699195 |
| [use of coffee grounds for production of pleurotus ostreatus (jacq.:fr.) kummer]. | studies were carried out to screen the industrial strain hk35 of pleurotus ostreatus for its ability to develop fruiting bodies in solid state cultivation using several substrates containing 17.8 to 55% coffee grounds. our results showed that only 55% of coffee grounds was used in the substrate without detecting changes in fruiting body or on its biological efficiency of production. the chemical analysis of the caffeine in the substrate showed that this compound decreased about 59% of the myceli ... | 2004 | 15709800 |
| the ability of white-rot fungi to degrade the endocrine-disrupting compound nonylphenol. | phanerochaete chrysosporium, pleurotus ostreatus, trametes versicolor and bjerkandera sp. bol13 were tested for their ability to degrade the endocrine-disrupting compound nonylphenol at an initial concentration of 100 mg l-1. the highest removals were achieved with t. versicolor and bjerkandera sp. bol13, which were able to degrade 97 mg l-1 and 99 mg l-1 of nonylphenol in 25 days of incubation, respectively. nonylphenol removal was associated with the production of laccase by t. versicolor, but ... | 2004 | 15735968 |
| [comparative study on the productivity of strains of pleurotus spp. in commercial cultivation]. | this paper describes the commercial production of two strains of pleurotus pulmonarius, selected in the laboratory for their rapid mycelial development and high production of basidiomata, and one commercial strain of pleurotus ostreatus. substrate preparation, impact of pathogens and environmental conditions necessary for the production and quality of the fruiting bodies required are discussed. | 2000 | 15762809 |
| degradation of lignin in pulp mill wastewaters by white-rot fungi on biofilm. | an investigation was conducted to explore the lignin-degrading capacity of attached-growth white-rot fungi. five white-rot fungi, phanerochaete chrysosporium, pleurotus ostreatus, lentinus edodes, trametes versicolor and s22, grown on a porous plastic media, were individually used to treat black liquor from a pulp and paper mill. over 71% of lignin and 48% of chemical oxygen demand (cod) were removed from the wastewater. several factors, including ph, concentrations of carbon, nitrogen and trace ... | 2005 | 15792583 |
| pharmacological study of oyster mushroom (pleurotus ostreatus) extract on isolated guinea pig trachea smooth muscle. | mushroom farm workers suffer from respiratory symptoms during the farming of mushrooms. the objective of this study was to analyze the effects of oyster mushroom (pleurotus ostreatus) extract (ome) on isolated guinea pig tracheal smooth muscle in vitro. isolated guinea pig tracheal tissue from 27 nonsensitized guinea pigs were studied. the ome was obtained from indoor mushroom growing fields and prepared as a 1:10 w/v aqueous solution. dose-related contractions of nonsensitized guinea pig trache ... | 2005 | 15793668 |
| interference of laccase in determination of cellobiose dehydrogenase activity of pleurotus ostreatus (florida) using dichlorophenol indophenol as the electron acceptor. | pleurotus ostreatus (florida), itcc 3308 produces approximately 9.0 u/ml extracellular cellobiose dehydrogenase (cdh) in cellulose medium after 7 days of growth. however, no activity could be detected if the assay was done with cellobiose as the substrate and 2,6-dichlorophenol indophenol (dpip) as the electron acceptor in absence of any laccase inhibitor. kinetic study showed that v(max)/k(m) value was very high for rdpip (reduced 2,6-dichlrophenol indophenol) oxidation by laccase. oxygen consu ... | 2005 | 15812859 |
| degradation of nitrocellulose by fungi. | three lignocellulolytic fungi, trametes versicolor, pleurotus ostreatus, and coprinus cinereus, and two cellulolytic fungi trichoderma reesei and chaetomium elatum were tested for their ability to degrade nitrocellulose. they were provided with different carbon and nitrogen sources in liquid cultures. nitrocellulose (n content above 12%) was added as nitrogen source (in solution in acetone) alongside amino acids or as sole n source. either starch or carboxy-methyl cellulose were provided as carb ... | 2005 | 15865147 |
| mushroom host influence on lycoriella mali (diptera: sciaridae) life cycle. | lycoriella mali fitch (diptera: sciaridae) infests mushroom crops early in the crop cycle. recent observations in mushroom houses indicated a difference in emergence time and size of adult l. mali developing on various strains of commercial mushrooms. samples of adult flies from isolated mushroom houses growing portabella mushrooms were significantly heavier then those from oyster mushroom houses, whereas flies from shiitake mushroom houses were lightest in weight. flies collected from isolated ... | 2005 | 15889722 |
| isolation of genes differentially expressed during the fruit body development of pleurotus ostreatus by differential display of rapd. | to analyze genes involved in fruit body development of pleurotus ostreatus, mrnas from three different developmental stages: i.e., vegetative mycelium, primordium, and mature fruit body, were isolated and reverse-transcribed to cdnas. one hundred and twenty random pcr amplifications were performed with the cdnas, which generated 382, 394, 393 cdna fragments from each developmental stage. from these fragments, four cdna clones specifically expressed in primordium or mature fruit body were detecte ... | 2005 | 15899417 |
| [137cs distribution and accumulation in the tissues of cultivated fungi (pleurotus ostreatus)]. | the authors examined 137cs accumulation and distribution in different structures and tissues of pleurotus ostreatus cultivated under laboratory conditions. the fungi were shown to concentrate 137cs. a higher concentrations of the radionuclides in the fungi compared to their substrate is manifested at the first stages of the fruit body formation, the maximum content of 137cs is accumulated by fungi in the middle of bearing stage. the fungus tissues are different by their accumulative capacity as ... | 2016 | 15906870 |
| 3-o-alkyl-d-glucose derivatives induce fruit bodies of pleurotus ostreatus. | amphipathic glucose derivatives 3-o-octyl- and 3-o-decyl-d-glucose were synthesized, and the ability to induce fruit bodies on pleurotus ostreatus was examined. surfactants such as chaps, chapso, mega-8, mega-9, and mega-10 were also assayed. the result was that both glucose derivatives could stimulate fruiting on p. ostreatus, while none of the surfactants assayed in this study stimulated the fruiting of p. ostreatus. these results suggest that sugar moiety is necessary for amphipathic compound ... | 2005 | 15912955 |
| temporal and spatial expression of ostreolysin during development of the oyster mushroom (pleurotus ostreatus). | in the mushrooms pleurotus ostreatus and agrocybe aegerita, expression of the hemolytic proteins ostreolysin and aegerolysin, which belong to the aegerolysin family, has been shown to be initiated specifically during formation of primordia and fruiting bodies. we used rabbit anti-ostreolysin and fluorescent rhodamine-labelled secondary goat antibodies for monitoring ostreolysin and aegerolysin in situ during the mushrooms' development. in parallel, the protein level in developing tissues was mon ... | 2005 | 15912956 |
| degradation of lignocellulose by pleurotus ostreatus in the presence of copper, manganese, lead and zinc. | pleurotus ostreatus produces the cellulolytic and hemicellulolytic enzymes endo-1,4-beta-glucanase, exo-1,4-beta-glucanase, 1,4-beta-glucosidase, endo-1,4-beta-xylanase, 1,4-beta-xylosidase, endo-1,4-beta-mannanase and 1,4-beta-mannosidase and ligninolytic enzymes mn-peroxidase and laccase during growth on wheat straw in the presence and absence of cu, mn, pb, and zn. this is the first report concerning endo-1,4-beta-mannanase in p. ostreatus. although the concentrations of trace metals in wheat ... | 2013 | 15921894 |
| [comparative analysis of pleurotus ostreatus natural isolates]. | a comparative analysis is performed of the polymorphism of the pleurotus ostreatus (fr.) kumm naturally occurring strains isolated from the natural substrates found in two geographically remote russian natural preserves, the central arboreal biosphere tver state preserve (cabtsp) and the moscow state university zvenigorod biological station (zbs, moscow oblast), and within the city of moscow. the results of the frequency analysis for the isozyme loci alleles and for the sexual and vegetative inc ... | 2006 | 15938400 |
| pleurostrin, an antifungal peptide from the oyster mushroom. | a 7kda peptide, with inhibitory activity on mycelial growth in the fungi fusaerium oxysporum, mycosphaerella arachidicola and physalospora piricola, was isolated from fresh fruiting bodies of the oyster mushroom. the isolation procedure entailed extraction with an aqueous buffer, ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel and gel filtration by fast protein liquid chromatography on superdex 75. the protein was unadsorbed on deae-cellulose and adsor ... | 2005 | 15941607 |
| a low-molecular mass ribonuclease from the brown oyster mushroom. | a ribonuclease, with a molecular mass of 9 kda and an n-terminal sequence resembling the sequence of a fragment of trna/rrna cytosine-c5-methylase and a fragment of a alanyl-trna synthetase, was isolated from fresh fruiting bodies of the brown oyster mushroom pleurotus ostreatus. the ribonuclease was purified using a very simple protocol that comprised ion-exchange chromatography on carboxymethyl (cm)-cellulose and affinity chromatography on affi-gel blue gel. subsequent gel filtration by fast p ... | 2005 | 15946190 |
| microbial degradation of cellulose wastes in continuous bioreactors. | this paper reports on experiments concerning the biodegradation of cellulose wastes from wine-producing industries, as well as from cereal crops processing in agricultural farms, using cellulolytic bacterial and fungal species immobilized on radiopolymerized hydrogels. there are described three types of such bioreactors and their main features. the immobilization procedures have been achieved using the following species of microorganisms: bacillus subtilis and b. licheniformis, from bacteria, as ... | 2001 | 15954586 |
| a carbon-carbon-coupled dimeric bergenin derivative biotransformed by pleurotus ostreatus. | a novel c-c-coupled dimer derivative of bergenin was produced by the biotransformation of cultured mycelia of white rot fungus pleurotus ostreatus. its structure was elucidated by detailed spectroscopic analysis. | 2005 | 15993599 |
| laccase production using pleurotus ostreatus 1804 immobilized on puf cubes in batch and packed bed reactors: influence of culture conditions. | the feasibility of laccase production by immobilization of pleurotus ostreatus 1804 on polyurethane foam (puf) cubes with respect to media composition was studied in both batch and reactor systems. enhanced laccase yield was evidenced due to immobilization. a relatively high maximum laccase activity of 312.6 u was observed with immobilized mycelia in shake flasks compared to the maximum laccase activity of free mycelia (272.2 u). it is evident from this study that the culture conditions studied, ... | 2005 | 15995650 |
| recombinant expression of pleurotus ostreatus laccases in kluyveromyces lactis and saccharomyces cerevisiae. | heterologous expression of pleurotus ostreatus poxc and poxa1b laccases in two yeasts, kluyveromyces lactis and saccharomyces cerevisiae, was performed. both transformed hosts secreted recombinant active laccases, although k. lactis was much more effective than s. cerevisiae. rpoxa1b transformants always had higher secreted activity than rpoxc transformants did. the lower tendency of k. lactis with respect to s. cerevisiae to hyperglycosylate recombinant proteins was confirmed. recombinant lacca ... | 2005 | 16021485 |
| a new method for screening and isolation of hypersecretion mutants in aspergillus niger. | although filamentous fungi have a unique property of secreting a large amount of homologous extracellular proteins, the use of filamentous fungi as hosts for the production of heterologous proteins is limited because of the low production levels that are generally reached. here, we report a general screening method for the isolation of mutants with increased protein production levels. the screening method makes use of an aspergillus niger strain that lacks the two major amylolytic enzymes, gluco ... | 2006 | 16021486 |
| novel antimutagenic factors derived from the edible mushroom agrocybe cylindracea. | studies have shown that certain foods contain compounds with antigenotoxic activities. here, we ask if dried powders and/or extracts from three edible mushrooms, agrocybe cylindracea, lentinula edodes and pleurotus ostreatus, have a mitigating effect on genotoxicity. we used two in vivo assays: the drosophila dna repair test and the drosophila wing spot test (also known as smart) which measures somatic mutation and recombination. eight carcinogens were tested with the mushroom powders: 2-aaf, af ... | 2005 | 16095952 |
| effect of ph on the pore forming activity and conformational stability of ostreolysin, a lipid raft-binding protein from the edible mushroom pleurotus ostreatus. | ostreolysin, a pore-forming protein from the edible oyster mushroom (pleurotus ostreatus), is a member of the aegerolysin protein family, a novel group of small acidic proteins found in bacteria, molds, mushrooms, and plants. it binds to lipid rafts and interacts specifically with cholesterol-rich lipid domains. in this study, ostreolysin was classified as a single-domain all-beta-structured protein on the basis of cdna sequencing. ph-induced and thermally induced unfolding of ostreolysin was st ... | 2005 | 16101298 |
| detection and recovery of hydrolytic enzymes from spent compost of four mushroom species. | to evaluate the potential of using the enzymes from spent mushroom compost (smc) as an industrial enzyme, the production of alpha-amylase, cellulase, beta-glucosidase, laccase, and xylanase was determined from the smc of four edible mushroom species (pleurotus ostreatus, lentinula edodes, flammulina velutipes and hericium erinaceum). among the tested smc, the smc of l. edodes showed the highest enzyme activity in alpha-amylase (229 nkat/g), cellulase (759 nkat/g) and beta-glucosidase (767 nkat/g ... | 2005 | 16110912 |
| estimation of bound and free fractions of lignocellulose-degrading enzymes of wood-rotting fungi pleurotus ostreatus, trametes versicolor and piptoporus betulinus. | liquid cultures with cellulose and solid state fermentation cultures on wheat straw of the white-rot fungi pleurotus ostreatus and trametes versicolor and the brown-rot fungus piptoporus betulinus were assayed for the free and solid fraction-bound activity of lignocellulose-degrading enzymes. the majority of the ligninolytic enzymes laccase and mn peroxidase was detected in the free fraction of p. ostreatus and t. versicolor. the endocleaving enzymes endo-1,4-beta-glucanase, endo-1,4-beta-mannan ... | 2006 | 16125911 |
| identification and functional characterisation of ctr1, a pleurotus ostreatus gene coding for a copper transporter. | copper homeostasis is crucial for the maintenance of life. in lignin-degrading fungi, copper is essential for the phenol oxidase enzymes that provide this activity. in this paper we report the characterization of a gene (ctr1) coding for a copper transporter in the white rot fungus pleurotus ostreatus. the gene was identified in a cdna library constructed from 4-day-old vegetative mycelium grown in liquid culture. the results presented here demonstrate that: (1) ctr1 functionally complements the ... | 2005 | 16133162 |
| subcellular distribution of aluminum, bismuth, cadmium, chromium, copper, iron, manganese, nickel, and lead in cultivated mushrooms (agaricus bisporus and pleurotus ostreatus). | in this work, the distribution of nine metals in two types of cultivated mushroom had been investigated. for agaricus bisporus, the biomass was separated into caps and stalks, and for pleurotus ostreatus, the entire mushrooms were taken for analysis. electrothermal atomic absorption spectrometry was used for total element determination in acid digests. for accuracy checking, the certified reference material (nist 1,571, citrus leaves) was analyzed. the results obtained for the two fungi species ... | 2005 | 16141474 |
| molecular breeding of white rot fungus pleurotus ostreatus by homologous expression of its versatile peroxidase mnp2. | using a dna-mediated transformation technique, a molecular breeding approach to isolate pleurotus ostreatus strains with enhanced productivity of its versatile peroxidase mnp2 was conducted. a recombinant mnp2 construct under the control of p. ostreatus sdi1 expression signals was introduced into the wild-type p. ostreatus strain by cotransformation with a carboxin-resistant marker plasmid. a total of 32 transformants containing the recombinant mnp2 sequence were isolated in a screening with spe ... | 2006 | 16163536 |
| laccase-mediated remazol brilliant blue r decolorization in a fixed-bed bioreactor. | a crude laccase mixture preparation from pleurotus ostreatus cultures supplemented with copper and ferulic acid was used to decolorize the anthraquinonic dye remazol brilliant blue r (rbbr). performance of this enzymatic system was tested, and a maximum of 70% decolorization was achievable under optimal conditions. the crude preparation was immobilized by entrapment in copper alginate beads attaining 65% yield of laccase activity. stability of the immobilized laccases was remarkably increased in ... | 2015 | 16209547 |
| biosynthesis of p-anisaldehyde by the white-rot basidiomycete pleurotus ostreatus. | the white-rot basidiomycete pleurotus ostreatus produced sweet flavor compounds on a liquid medium. the major and minor compounds identified by gc-ms analysis were p-anisaldehyde (4-methoxybenzaldehyde) and 3-chloro-p-anisaldehyde (3-chloro-4-methoxybenzaldehyde), respectively. p-anisaldehyde was only produced under static culture conditions. differences in the type and quantity of flavor compounds produced among wild strains of p. ostreatus were observed. aryl alcohol oxidase and manganese pero ... | 2002 | 16233188 |
| biodegradation of wood in dual cultures of selected two fungi determined by chopstick method. | wood was more effectively degraded in dual cultures of pycnoporus coccineus with either flammulina velutipes, pleurotus ostreatus or polyporus arcularius than in their single cultures based on the weight losses of chopsticks. the similar effects were attained in dual cultures of the selected basidiomycetes and various soil fungi. | 2003 | 16233468 |
| response surface analysis of solid state growth of pleurotus ostreatus mycelia utilizing whey permeate. | a novel approach to utilizing whey permeate, the cultivation of mycelia of the edible mushroom pleurotus ostreatus, is introduced. response surface analysis (rsa) was successfully applied to determine the combination of substrate concentration, temperature and ph that would result in a maximal mycelial extension rate under solid state cultivation. the conditions to maximize the mycelial extension rate were predicted to be 44 g lactose l(-1), ph 6.0 and 24.2 degrees c. subsequent verification of ... | 2005 | 16245171 |
| [expression of a laccase gene from pleurotus ostreatus in pichia pastoris and characterization of the recombinant enzyme]. | a pleuotus ostreatus laccase gene was cloned by rt-pcr and designated as lccpol. its sequence was submitted to genbank with the accession number ay450404 obtained. the open reading frame was transformed into three pichia pastoris strains gs115, km71 and smd1168, respectively, under control of the aox1 promoter by using the vector phbm906. lccpo1 can be expressed by all three p. pastoris recombinant strains. three different strategies for shake-flask cultures were compared: (1) (25 degrees c, 1.0 ... | 2005 | 16245886 |
| leadbetterella byssophila gen. nov., sp. nov., isolated from cotton-waste composts for the cultivation of oyster mushroom. | a bacterial strain, designated 4m15t, was isolated from cotton-waste composts used as mushroom cultivation in south korea. properties of this isolate were studied on the basis of physiological and biochemical characteristics, fatty acid profile, isoprenoid quinone, dna g+c content and phylogenetic position based on 16s rrna gene sequence analysis. the strain was found to form a distinct phylogenetic lineage related to the family 'flexibacteraceae' within the phylum 'bacteroidetes'. no recognized ... | 2005 | 16280486 |
| structure selectivity in degradation and translocation of polychlorinated biphenyls (delor 103) with a pleurotus ostreatus (oyster mushroom) culture. | the biodegradation of polychlorinated biphenyls (pcbs), formerly applied in technical mixtures called delor 103, was realized by the white rot fungus pleurotus ostreatus. besides the efficiency of degradation the translocation of pcbs into the fruit bodies grown during cultivation on straw were of special interest. the selectivity of pcbs degradation is discussed on the basis of structural features of the individual congeners. the most important structural factors affecting the degradation effic ... | 2005 | 16291407 |
| growth and production of laccases by the ligninolytic fungi, pleurotus ostreatus and botryosphaeria rhodina , cultured on basal medium containing the herbicide, scepter (imazaquin). | the herbicide, scepter, whose active principle is imazaquin, is commonly used in soybean farming to combat wide-leaf weeds. the basidiomycete, pleurotus ostreatus , and the ascomycete, botryosphaeria rhodina , were evaluated for their growth and laccase production when cultured on basal media containing scepter. both fungi could grow on the herbicide when cultivated in solid and submerged liquid culture in the presence of scepter at concentrations of 0-6% (v/v) for p. ostreatus , and up to 0-50% ... | 2005 | 16304708 |
| comparative studies of extracellular fungal laccases. | various basidiomycetes, ascomycetes, and deuteromycetes, grown in a sugar-rich liquid medium, were compared for laccase-producing ability and for the inducing effect of 2,5-xylidine on laccase production. clear stimulation of the extracellular enzyme formation by xylidine was obtained in the cultures of fomes annosus, pholiota mutabilis, pleurotus ostreatus, and trametes versicolor, whereas rhizoctonia praticola and botrytis cinerea were not affected by the xylidine, and in the case of podospora ... | 1984 | 16346649 |
| fruiting body formation from regenerated mycelium of pleurotus ostreatus protoplasts. | fruiting bodies were induced from mycelium regenerated from pleurotus ostreatus protoplasts. mycelia originated from protoplasts conformed to parental strain mycelia in morphology. six strains selected at random from the dikaryotic regenerants were able to form normal fruiting bodies, yielding 18% more than the parent. | 1985 | 16346733 |
| yellow blotch of pleurotus ostreatus. | yellow blotch disease of the oyster mushroom (pleurotus ostreatus) was first observed in a commercial mushroom farm in california in 1983. the disease, caused by pseudomonas agarici, is characterized by primordia, with yellow droplets on their surface, which become stunted, yellow to orange, and deformed as they mature. | 1985 | 16346953 |
| chemical composition of the edible mushroom pleurotus ostreatus produced by fermentation. | pleurotus ostreatus ;florida' was grown in submerged liquid culture. the biomass yield of the fungus, grown for 3 days in 2-liter fermentors, where the mycelial pellets measuring 5 mm in diameter were formed, was 11.7 g (dry weight)/liter. comparing the chemical constituents of fruiting bodies produced on cotton straw and mycelial pellets revealed several similarities in total nitrogen, protein, glycogen, fatty acids, rna, and ash content. differences were observed in the contents of six amino a ... | 1986 | 16347090 |
| lignocellulose degradation during solid-state fermentation: pleurotus ostreatus versus phanerochaete chrysosporium. | lignocellulose degradation and activities related to lignin degradation were studied in the solid-state fermentation of cotton stalks by comparing two white rot fungi, pleurotus ostreatus and phanerochaete chrysosporium. p. chrysosporium grew vigorously, resulting in rapid, nonselective degradation of 55% of the organic components of the cotton stalks within 15 days. in contrast, p. ostreatus grew more slowly with obvious selectivity for lignin degradation and resulting in the degradation of onl ... | 1992 | 16348683 |
| effect of manganese on lignin degradation by pleurotus ostreatus during solid-state fermentation. | lignin degradation by pleurotus ostreatus was studied under solid-state fermentation (ssf) in chemically defined medium containing various levels of mn. degradation of [c]lignin prepared from cotton branches to soluble products, as well as its mineralization to co(2), was enhanced by the addition of mn. the effect of malonate on lignin mineralization was most marked during the first 10 days of ssf, in a treatment amended with 73 mum mn. a high concentration of mn (4.5 mm) caused inhibition of bo ... | 1993 | 16349112 |
| production of oxalic acid by a wood-rotting fungus. | the wood-rotting fungus pleurotus ostreatus nrrl 2366 was grown successfully in submerged shaker cultures in which it produced oxalic acid from simple carbohydrates as efficiently as did aspergillus niger. p. ostreatus also produced oxalic acid from mixtures of sawdust and caco(3), and from the solid residue from the acid hydrolysis of wood when the culture was supplemented with inorganic nutrients. a. niger produced oxalic acid from the liquid hydrolysate. | 1963 | 16349634 |
| an aqueous polysaccharide extract from the edible mushroom pleurotus ostreatus induces anti-proliferative and pro-apoptotic effects on ht-29 colon cancer cells. | anti-proliferative and pro-apoptotic activities of fractions of pleurotus ostreatus were examined using ht-29 colon cancer cells in vitro. a hot-water-soluble fraction of the mycelium of the liquid cultured mushroom was partially isolated and chemically characterized as a low-molecular-weight alpha-glucan. ht-29 cells were exposed to the different isolates and significant inhibition of proliferation was obtained in a dose-dependent manner. proliferation inhibition was shown to be the result of a ... | 2006 | 16413114 |
| effect of copper and manganese ions on activities of laccase and peroxidases in three pleurotus species grown on agricultural wastes. | copper (cu2+) and manganese (mn2+) ions influenced laccase (lac) and peroxidase production in pleurotus eryngii, pleurotus ostreatus, and pleurotus pulmonarius. in p. eryngii, the optimum cu2+ concentration for lac production was 1 mm and for peroxidases 10 mm, and mn2+ concentration of 5 mm led to peaks of lac and peroxidase activity. in p. ostreatus hai 493, the highest level of lac activity was at cu2+ concentrations of 1 and 10 mm and mn2+ concentration of 1 mm, respectively. the absence of ... | 2006 | 16415481 |
| enzyme activities and chemical changes in wet olive cake after treatment with pleurotus ostreatus or eisenia fetida. | a laboratory experiment was conducted to evaluate the enzyme activities and chemical changes recorded in a recalcitrant phenolic-rich waste after treatment with pleurotus ostreatus or eisenia fetida. the waste used was wet olive cake (alperujo in spanish), a waste produced in huge amounts by the olive oil industry. both p. ostreatus and e. fetida were very effective in removing phenolic compounds, the initial concentration in the wet olive cake being reduced in both cases by around 90%. laccase ... | 2006 | 16453175 |
| evaluation of pretreatment with pleurotus ostreatus for enzymatic hydrolysis of rice straw. | the effects of biological pretreatment of rice straw using four white-rot fungi (phanerochaete chrysosporium, trametes versicolor, ceriporiopsis subvermispora, and pleurotus ostreatus) were evaluated on the basis of quantitative and structural changes in the components of the pretreated rice straw as well as susceptibility to enzymatic hydrolysis. of these white-rot fungi, p. ostreatus selectively degraded the lignin fraction of rice straw rather than the holocellulose component. when rice straw ... | 2005 | 16473773 |
| evaluation of synthetic dye decolorization capacity in ischnoderma resinosum. | the little studied white rot fungus ischnoderma resinosum was tested for its ability to decolorize seven different synthetic dyes. the strain efficiently decolorized orange g, amaranth, remazol brilliant blue r, cu-phthalocyanin and poly r-478 on agar plates and in liquid culture at a relatively high concentration of 2-4 and 0.5-1 g l(-1), respectively. malachite green and crystal violet were decolorized to a lower extent up to the concentration of 0.1 g l(-1). decolorization capacity of i. resi ... | 2006 | 16491363 |
| a highly efficient polyethylene glycol-mediated transformation method for mushrooms. | a highly efficient transformation system mediated by polyethylene glycol was developed for the cultivated mushroom pleurotus ostreatus. eighty to 180 integrative and stable-resistant colonies appeared per mug of dna per 10(7) viable protoplasts in a transformation experiment with the hygromycin b phosphotransferase gene (hph), which is about 40-1800 times higher than that previously reported in p. ostreatus. one hundred to 150 transformants emitting green fluorescence were observed per mug of dn ... | 2006 | 16499607 |
| ureibacillus suwonensis sp. nov., isolated from cotton waste composts. | the taxonomic position of two spore-forming strains 6t19t and 6t29, isolated from cotton composts for the cultivation of oyster mushroom (pleurotus ostreatus), was investigated by a polyphasic approach. cells of strains 6t19(t) and 6t29 were rod-shaped, gram-negative and strictly aerobic. sequencing and comparative analyses for the 16s rrna genes of these strains clearly showed their phylogenetic affiliation to the genus ureibacillus. their closest relatives ureibacillus thermosphaericus and ure ... | 2006 | 16514046 |
| [yellow laccase from the fungus pleurotus ostreatus d1: purification and characterization]. | yellow laccase was isolated from a solid-phase culture of the fungus pleurotus ostreatus d1 and characterized. it is a copper-containing enzyme with molecular weight 64 kda. its absorption spectrum lacks the maximum at 610 nm, characteristic of fungal laccases and corresponding to type i copper atom. the optimum ph values for the enzyme were determined. they proved to be: 7.0 for syringaldazine, 8.0 for pyrocatechol, and 4.0 for 2,2'-azine-bis-(3-ethylbenzothiazoline-6-sulfonate and 2,6-dimethox ... | 2015 | 16521579 |
| genetic linkage map and expression analysis of genes expressed in the lamellae of the edible basidiomycete pleurotus ostreatus. | pleurotus ostreatus is an industrially cultivated basidiomycete with nutritional and environmental applications. its genome contains 35 mbp organized in 11 chromosomes. there is currently available a genetic linkage map based predominantly on anonymous molecular markers complemented with the mapping of qtls controlling growth rate and industrial productivity. to increase the saturation of the existing linkage maps, we have identified and mapped 82 genes expressed in the lamellae. their manual an ... | 2006 | 16531085 |
| mineralization of polycyclic aromatic hydrocarbons by the white rot fungus pleurotus ostreatus. | the white rot fungus pleurotus ostreatus was able to mineralize to (sup14)co(inf2) 7.0% of [(sup14)c]catechol, 3.0% of [(sup14)c]phenanthrene, 0.4% of [(sup14)c]pyrene, and 0.19% of [(sup14)c]benzo[a]pyrene by day 11 of incubation. it also mineralized [(sup14)c]anthracene (0.6%) much more slowly (35 days) and [(sup14)c]fluorene (0.19%) within 15 days. p. ostreatus did not mineralize fluoranthene. the activities of the enzymes considered to be part of the ligninolytic system, laccase and manganes ... | 1996 | 16535219 |
| initial oxidation products in the metabolism of pyrene, anthracene, fluorene, and dibenzothiophene by the white rot fungus pleurotus ostreatus. | the initial metabolites in the degradation of pyrene, anthracene, fluorene, and dibenzothiophene by pleurotus ostreatus were isolated by high-pressure liquid chromatography and characterized by uv-visible, gas-chromatographic, mass-spectrometric, and (sup1)h nuclear magnetic resonance spectral techniques. the metabolites from pyrene, dibenzothiophene, anthracene, and fluorene amounted to 45, 84, 64, and 96% of the total organic-solvent-extractable metabolites, respectively. pyrene was metabolize ... | 1996 | 16535361 |
| production and purification of remazol brilliant blue r decolorizing peroxidase from the culture filtrate of pleurotus ostreatus. | an extracellular h(inf2)o(inf2)-requiring remazol brilliant blue r (rbbr) decolorizing enzymatic activity was found in the culture medium of pleurotus ostreatus. the enzymatic activity was maximally obtained in idiophase, and the optimum c/n ratio was 24. high c/n ratios repressed the enzymatic activity, and addition of veratryl alcohol had no effect on the production of enzyme. the enzyme was purified by ammonium sulfate fractionation, sephacryl s-200 hr chromatography, deae sepharose cl-6b chr ... | 1997 | 16535590 |
| enzymatic mechanisms involved in phenanthrene degradation by the white rot fungus pleurotus ostreatus. | the enzymatic mechanisms involved in the degradation of phenanthrene by the white rot fungus pleurotus ostreatus were examined. phase i metabolism (cytochrome p-450 monooxygenase and epoxide hydrolase) and phase ii conjugation (glutathione s-transferase, aryl sulfotransferase, udp-glucuronosyltransferase, and udp-glucosyltransferase) enzyme activities were determined for mycelial extracts of p. ostreatus. cytochrome p-450 was detected in both cytosolic and microsomal fractions at 0.16 and 0.38 n ... | 1997 | 16535634 |
| growth of pleurotus ostreatus on wheat straw and wheat-grain-based media: biochemical aspects and preparation of mushroom inoculum. | mycelial growth, intracellular activity of proteases, laccases and beta-1,3-glucanases, and cytoplasmic protein were evaluated in the vegetative phase of pleurotus ostreatus grown on wheat straw and in wheat-grain-based media in petri dishes and in bottles. the productivity of the wheat straw and wheat-grain-based spawn in cylindrical polyethylene bags containing 5 kg of chopped straw was also determined. we observed high activity of proteases and high content of intracellular protein in culture ... | 2006 | 16586105 |
| lovastatin production by pleurotus ostreatus: effects of the c:n ratio. | the types of carbon source and nitrogen source used as well as the c:n ratio in the medium influenced lovastatin production by pleurotus ostreatus. the maximum value of the lovastatin yield was obtained in a medium that contained organic nitrogen. | 2013 | 16610224 |
| se-enriched mycelia of pleurotus ostreatus: distribution of selenium in cell walls and cell membranes/cytosol. | the incorporation of se to fungi has been studied, focusing on element distribution among different cellular compartments and, in particular, polysaccharide structures contained in cell walls. se-enriched mycelia of pleurotus ostreatus were obtained in submerged cultures. the incorporation of selenium from the growth medium to mycelia was observed with the relative distribution between cytosol plus cell membranes fraction (ccm) and cell walls fraction (cw) of about 44 and 56%, respectively. ccm ... | 2006 | 16637705 |
| influence of dimethyl sulfoxide on extracellular enzyme production by pleurotus ostreatus. | dimethyl sulfoxide (dmso) is commonly used as a co-solvent to dissolve poorly water-soluble biologically active agents to assess their biological activities such as for enzyme induction. the question addressed was whether dmso can be assumed to be an inert co-solvent. the influence of dmso on the production of extracellular enzymes by pleurotus ostreatus was investigated. dmso functioned as either an inducer or a repressor, depending on the enzyme studied. the production of laccase and endo-1,4- ... | 2006 | 16642304 |
| wild-gathered fungi for health and rural livelihoods. | fungi are a good source of digestible proteins and fibre, are low in fat and energy and make a useful contribution to vitamin and mineral intake. in terms of current dietary advice, 80 g fungi represent one portion of vegetables. dried fungi and concentrated extracts are also used as medicines and dietary supplements. some species show strong anti-tumour and antioxidant activity by enhancing various immune system functions and lowering cholesterol levels. nevertheless, there are also some safety ... | 2006 | 16672080 |
| [micromorphological features of pleurotus pulmonarius (fr.) quel. and p. ostreatus (jacq.) p. kumm. strains in pure and binary culture with yeasts]. | micromorphology of oyster mushrooms pleurotus ostreatus (jacq.) quel. and p. pulmonarius (fr.) quel. was studied in pure and binary culture with yeasts (cryptococcus laurentii 1629, rhodotorula minuta 2790, sporidiobolus salmonicolor (see text for symbol) 31a-11, candida krusie 3452, pichia holstii 3438). the cultures were cultivated on malt-agar and water agar. various mycelial structures were described: strands, rings, thin searching mycelium, clamps, crystals, head-like offshoots, mycelial fr ... | 2006 | 16737183 |
| the chemistry of the white rots of wood: the effect on wood substance of ganoderma applanatum (pers.) pat., fomes fomentarius (linn.) fr., polyporus adustus (willd.) fr., pleurotus ostreatus (jacq.) fr., armillaria mellea (vahl.) fr., trametes pini (brot.) fr., and polystictus abietinus (dicks.) fr. | 1932 | 16745007 | |
| anti-tumor and immunomodulating effects of pleurotus ostreatus mycelia-derived proteoglycans. | pleurotus ostreatus is one of the widely cultivated edible mushrooms. water-soluble proteoglycan fractions from p. ostreatus mycelia were purified by alcohol-precipitation, ion exchange and followed by gel permeation (sephadex g-100) chromatography. three neutral fractions were found, which had polysaccharide to protein ratios 14.2, 26.4 and 18.3, respectively. these fractions were tested for in vitro and in vivo immunomodulatory and anticancer effects on sarcoma-180-bearing mouse model. in vivo ... | 2006 | 16782541 |