| oyster mushroom laccase inhibits hepatitis c virus entry into peripheral blood cells and hepatoma cells. | there is no protective vaccine or effective drug against hepatitis c virus (hcv). sustained virological response to inf/ribavirin treatment regimen has an efficiency of about 50%. many patients worldwide have used traditional medicines and herbal medicine in particular. a laccase has been purified from oyster mushroom (pleurotus ostreatus) to homogeneity by deae affi-gel blue gel, cm-sephadex g-50 and sephadex g-100. the molecular weight of the laccase was about 58 kda in sds-page. the optimum p ... | 2010 | 20156183 |
| antimicrobial and antioxidant activities of mycelia of 10 wild mushroom species. | antimicrobial and antioxidant activities of mycelia obtained from 10 wild edible mushrooms-armillaria mellea, meripilus giganteus, morchella costata, morchella elata, morchella esculenta var. vulgaris, morchella hortensis, morchella rotunda, paxillus involutus, pleurotus eryngii, and pleurotus ostreatus-were investigated. for determination of antimicrobial activities of these mushrooms, ethanol extracts were examined with 11 test microorganisms by the agar well diffusion method. p. ostreatus and ... | 2010 | 20192845 |
| evaluation of holocellulase production by plant-degrading fungi grown on agro-industrial residues. | agaricus brasiliensis cs1, pleurotus ostreatus h1 and aspergillus flavus produced holocellulases when grown in solid and submerged liquid cultures containing agro-industrial residues, including sugar cane bagasse and dirty cotton residue, as substrates. these isolates proved to be efficient producers of holocellulases under the conditions used in this screening. bromatological analysis of agro-industrial residues showed differences in protein, fiber, hemicellulose, cellulose and lignin content. ... | 2010 | 20221846 |
| [development of a laminar bioadsorbent with hypertolerant phanerochaete chrysosporium to cd, ni and pb for wastewater treatment]. | the use of basidiomycetes for metal removal is an alternative to traditional methods. in this, the biomass acts as a natural ionic exchanger removing metals from solution. | 2010 | 20346296 |
| a semi-rational approach to engineering laccase enzymes. | in order to develop improved laccase-based bio-catalysts, semi-rational mutagenesis of the laccase poxa1b from pleurotus ostreatus was performed through a combination of directed evolution with elements of rational enzyme modification. the r4 laccase was prepared by joining mutations of previously selected poxa1b random variants. an enhancement of stability features was thus obtained, making the novel enzyme r4 more appropriate as scaffold for directed evolution. a library of 1000 randomly mutat ... | 2010 | 20467838 |
| enzyme production by solid substrate fermentation of pleurotus ostreatus and trametes versicolor on tomato pomace. | a process of solid state fermentation (ssf) on tomato pomace was developed with the white-rot fungi pleurotus ostreatus and trametes versicolor, using sorghum stalks as support. operative parameters (humidity, water activity, and size of substrate particles) guaranteeing a good colonization of tomato pomace by both fungi were defined and conditions for production at high titers of the industrially relevant enzymes laccase, xylanase and protease were identified. significant laccase activity level ... | 2011 | 20582639 |
| electrostatically self-assembled azides on zinc sulfide nanoparticles as multifunctional nanoprobes for peptide and protein analysis in maldi-tof ms. | a simple method to synthesize electrostatically self-assembled azides on zinc sulfide nanoparticles (zns-n(3) nps) was described and then it was further applied as a multifunctional nanoprobe such as enriching, desalting, accelerating and separation-/washing free nanoprobes for rapid analysis of peptides and proteins and microwave assisted tryptic digested proteins in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms). the zns-n(3) nps were characterized ... | 2010 | 20602933 |
| development of an efficient and simple method for conjugation of laccase to immunoglobulin and its characterization by enzyme immunoassay. | a new laccase-conjugated antibody is developed, containing laccase as an enzyme marker, obtained from culture supernatant of the basidiomycetous fungi pleurotus ostreatus. the efficacy of the laccase-conjugated antibody was demonstrated in indirect and direct enzyme immunoassay after using periodate and glutaraldehyde conjugation methods. the assay based on laccase-conjugated antibody is potent to detect antigens when compared with peroxidase conjugated antibody. | 2010 | 20623408 |
| characterization of pseudomonads isolated from decaying sporocarps of oyster mushroom. | pleurotus ostreatus is one of the most extensively cultivated mushrooms in the world; however, the success of cultivation often depends on the proliferation of different bacterial pathogens. pseudomonas tolaasii is thought as the major cause of brown blotch disease of agaricus bisporus and yellowing of pleurotus ostreatus. in this study we examined the pathogenicity and assessed the industrial damage causing effect of 41 pseudomonas strains isolated from deformed, yellowing oyster mushroom (p. o ... | 2011 | 20627228 |
| potential for bioremediation of agro-industrial effluents with high loads of pesticides by selected fungi. | wastewaters from the fruit packaging industry contain a high pesticide load and require treatment before their environmental discharge. we provide first evidence for the potential bioremediation of these wastewaters. three white rot fungi (wrf) (phanerochaete chrysosporium, trametes versicolor, pleurotus ostreatus) and an aspergillus niger strain were tested in straw extract medium (stem) and soil extract medium (sem) for degrading the pesticides thiabendazole (tbz), imazalil (imz), thiophanate ... | 2011 | 20635121 |
| both common and specialty mushrooms inhibit adhesion molecule expression and in vitro binding of monocytes to human aortic endothelial cells in a pro-inflammatory environment. | cardiovascular disease (cvd) is a leading cause of mortality in the united states as well as globally. epidemiological studies show that regular fruit and vegetable consumption reduces cvd risk, in part, due to antioxidant activity and immunomodulation since oxidative stress and inflammation are features of atherogenesis. accumulating evidence also shows that dietary fungi, viz., mushrooms, can protect against chronic disease by altering inflammatory environments such as those associated with cv ... | 2010 | 20637088 |
| an extract of the pleurotus ostreatus mushroom bolsters the glutathione redox system in various organs of aged rats. | this study is to investigate the putative effect of an extract of the oyster mushroom, pleurotus ostreatus, on reduced glutathione (gsh) and its metabolic enzymes in major organs of male albino rats. a significant (p < .05) decrease in the level of gsh was observed in liver, kidneys, heart, and brain of aged rats compared to young rats. activities of glutathione s-transferase (gst), glutathione reductase (gr), and glucose 6-phosphate dehydrogenase (g6pdh) were significantly (p < .05) lower in th ... | 2010 | 20673055 |
| lecanicillium fungicola: causal agent of dry bubble disease in white-button mushroom. | lecanicillium fungicola causes dry bubble disease in commercially cultivated mushroom. this review summarizes current knowledge on the biology of the pathogen and the interaction between the pathogen and its most important host, the white-button mushroom, agaricus bisporus. the ecology of the pathogen is discussed with emphasis on host range, dispersal and primary source of infection. in addition, current knowledge on mushroom defence mechanisms is reviewed. taxonomy: lecanicillium fungicola (pr ... | 2010 | 20695998 |
| an extract of the oyster mushroom, pleurotus ostreatus, increases catalase gene expression and reduces protein oxidation during aging in rats. | the objective of the present study was to address the effect of mushroom pleurotus ostreatus on the catalase (cat) gene expression and the protein carbonyls in liver and kidney of aged (24 months old) rats. | 2010 | 20727333 |
| white-rot fungal conversion of wheat straw to energy rich cattle feed. | in order to improve the digestibility and nutrient availability in rumen, wheat straw was subjected to solid state fermentation (ssf) with white-rot fungi (i.e. pleurotus ostreatus and trametes versicolor) and the fermented biomass (called myco-straw) was evaluated for biochemical, enzymatic and nutritional parameters. the fungal treatment after 30 days led to significant decrease (p < 0.05) in cell wall constituents viz, acid detergent fiber (adf), neutral detergent fiber (ndf), hemicellulose, ... | 2010 | 20734121 |
| optimization of laccase production by pleurotus ostreatus imi 395545 using the taguchi doe methodology. | production of laccase using a submerged culture of pleurotus orstreatus imi 395545 was optimized by the taguchi orthogonal array (oa) design of experiments (doe) methodology. this approach facilitates the study of the interactions of a large number of variables spanned by factors and their settings, with a small number of experiments, leading to considerable savings in time and cost for process optimization. this methodology optimizes the number of impact factors and enables to calculate their i ... | 2010 | 20806254 |
| agrobacterium tumefaciens mediated fused egfp-hph gene expression under the control of gpd promoter in pleurotus ostreatus. | a transformation system for the basidiomycete pleurotus ostreatus was established using agrobacterium-mediated infection. following p. ostreatus glyceraldehyde-3-phosphate dehydrogenase gene analysis, its promoter region including two introns was used as cis-regulatory element to drive expression of enhanced green fluorescent protein (egfp). as a selection marker, the hygromycin phosphotransferase (hph) gene cassette was used in the binary vector ppeh. mycelia without pretreatment were found to ... | 2010 | 20869218 |
| mitigation of arsenic-mediated renal oxidative stress in rat by pleurotus florida lectin. | oyster mushroom, pleurotus florida is regarded as one of the popular food with biopharmaceutical properties. here, the study aimed to investigate the antioxidative effects of mushroom (pleurotus florida) lectin against arsenic-induced nephrotoxicity in rats. animals were divided into four groups; group 1 was control. groups 2, 3 and 4 were exposed to arsenic (20 parts per million [ppm] in drinking water), arsenic plus oral supplementation of ascorbic acid (25 mg/kg body weight) and arsenic plus ... | 2010 | 20876158 |
| pleurotus ostreatus inhibits colitis-related colon carcinogenesis in mice. | colorectal cancer is one of the leading causes of cancer deaths in both men and women in the world. however, colon cancer can be prevented to some extent by consumption of edible natural products with chemopreventive properties. therefore, we investigated, whether edible mushroom pleurotus ostreatus (po) has chemopreventive effect on inflammation-associated colon carcinogenesis induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip) and promoted by dextran sodium sulfate (dss). po trea ... | 2010 | 20878085 |
| medicinal and edible lignicolous fungi as natural sources of antioxidative and antibacterial agents. | the antioxidant activity of organic extracts of eight fungal species, ganoderma lucidum, ganoderma applanatum, meripilus giganteus, laetiporus sulphureus, flammulina velutipes, coriolus versicolor, pleurotus ostreatus and panus tigrinus, was evaluated for free radical (dpph· and oh·) scavenging capacity and an effect on lipid peroxidation, and the antibacterial activity was tested by the agar well diffusion method. the highest dpph· scavenging activity was found in the methanol extract of g. app ... | 2010 | 20878697 |
| commonly consumed and specialty dietary mushrooms reduce cellular proliferation in mcf-7 human breast cancer cells. | worldwide, over one million women will be newly diagnosed with breast cancer in the next year. moreover, breast cancer is the second leading cause of cancer death in the usa. an accumulating body of evidence suggests that consumption of dietary mushrooms can protect against breast cancer. in this study, we tested and compared the ability of five commonly consumed or specialty mushrooms to modulate cell number balance in the cancer process using mcf-7 human breast cancer cells. hot water extracts ... | 2010 | 20921274 |
| particle and naked rna mycoviruses in industrially cultivated mushroom pleurotus ostreatus in china. | many cultivated mushroom strains, such as pleurotus ostreatus td300, displayed symptoms of degeneration. a spherical virus posv and four dsrna segments were extracted from mycelium of p. ostreatus td300. posv had a diameter of 23 nm and encapsidated a 2.5kb dsrna segment with coat proteins whose molecular weights were 39 kda and 30 kda. four dsrna segments were 8.2 kb, 2.5 kb, 2.0 kb, and 1.1 kb in size, respectively. the 1.1 kb dsrna segment often escaped detection. the cdna and the amino acid ... | 2010 | 20943162 |
| assessment of heavy metals in some wild edible mushrooms collected from yunnan province, china. | eight heavy metals (cu, zn, fe, mn, cd, cr, ni, and pb) in 14 different wild-growing edible mushroom species (coprinus comatus, voluariella volvacea, pleurotus nebrodensis, hypsizigus marmoreus, hericium erinaceus, agrocybe aegerita, lenfinus edodes, collybia velutipes, agaricus bisporus, russula albida, clitocybe conglobata, pleurotus eryngii, lepista sordida, and pleurotus ostreatus) collected from yunnan province, china, were determined by inductively coupled plasma-atomic emission spectromet ... | 2010 | 20976551 |
| extraction and application of laccases from shimeji mushrooms (pleurotus ostreatus) residues in decolourisation of reactive dyes and a comparative study using commercial laccase from aspergillus oryzae. | oxidases are able to degrade organic pollutants; however, high costs associated with biocatalysts production still hinder their use in environmental biocatalysis. our study compared the action of a commercial laccase from aspergillus oryzae and a rich extract from pleurotus ostreatus cultivation residues in decolourisation of reactive dyes: drimaren blue x-3lr (dmblr), drimaren blue x-bln (dmbbln), drimaren rubinol x-3lr (dmr), and drimaren blue c-r (rbbr). the colour removal was evaluated by co ... | 2010 | 21052547 |
| in vitro antitumor and immunomodulatory effects of the protein pcp-3a from mushroom pleurotus citrinopileatus. | a nonlectin glycoprotein (pcp-3a) newly isolated from the fruit body of edible golden oyster mushroom pleurotus citrinopileatus has been shown to be growth inhibitory against human myeloid leukemic u937 cells in a previous report. there is a well-recognized relation between antitumor activity and immunomodulation. the immunomodulatory activity of pcp-3a was therefore assessed in the present study. human mononuclear cells (mnc) and the cd4(+) t lymphocytes isolated from them were stimulated separ ... | 2010 | 21053905 |
| in vivo bioavailability of selenium in enriched pleurotus ostreatus mushrooms. | the in vivo bioavailability of se was investigated in enriched pleurotus ostreatus mushrooms. a bioavailability study was performed using 64 wistar male rats separated in 8 groups and fed with different diets: without se, with mushrooms without se, with enriched mushrooms containing 0.15, 0.30 or 0.45 mg kg(-1) se and a normal diet containing 0.15 mg kg(-1) of se using sodium selenate. the experiment was performed in two periods: depletion (14 days) and repletion (21 days), according to the asso ... | 2010 | 21069148 |
| production of xyloglucanolytic enzymes by trichoderma viride, paecilomyces farinosus, wardomyces inflatus, and pleurotus ostreatus. | different conditions of culture medium, incubation time, concentration and surfactant were tested to determine xyloglucanase activity. trichoderma viride, paecilomyces farinosus, wardomyces inflatus and pleurotus ostreatus showed increased xyloglucanase activities when the fungi grown on microcrystalline cellulose as the sole carbon source. endoxyloglucanase activity increased with the growth of the fungi and reached a peak on day 14 of incubation, practically 95% of the activity was associated ... | 2002 | 21156511 |
| α-(1 → 3)-d-glucans from fruiting bodies of selected macromycetes fungi and the biological activity of their carboxymethylated products. | purpose of work: to show biological activity of carboxymethylated α-(1 → 3)-d-glucans isolated from the selected macromycetes fungi on human tumor and normal cells. water-insoluble, alkali-soluble polysaccharides (wip) were isolated from fruiting bodies of four macromycetes fungi: lentinus edodes, pleurotus ostreatus, piptoporus betulinus and laetiporus sulphureus. the structure of the polysaccharides was determined using composition analysis, methylation analysis, fourier transform infrared spe ... | 2010 | 21188616 |
| search for new naturally occurring strains of pleurotus to improve yields. pleurotus albidus as a novel proposed species for mushroom production. | background: the species of genus pleurotus are worldwide cultivated. aims: to evaluate growth, yield production and morphological variations of fruiting bodies obtained from the cultivation of fourteen naturally occurring pleurotus strains isolated from argentina. methods: the strains growth was tested at different temperatures on nobles' medium. substrates assayed were: supplemented salix sawdust, supplemented and non supplemented wheat straw. the species studied were pleurotus albidus, pleurot ... | 2011 | 21241817 |
| morphology and laccase production of white-rot fungi grown on wheat bran flakes under semi-solid-state fermentation conditions. | in this paper, we studied the laccase production and the growth morphology of different white-rot fungi, i.e. pleurotus ostreatus, trametes pubescens, cerrena unicolor and trametes versicolor, cultured under semi-solid-state fermentation conditions using wheat bran flakes as a natural low-cost support substrate. trametes versicolor exhibited the highest laccase activity per gram of total dry matter, followed by p. ostreatus (63.5 and 58.2ug(-1) , respectively). in addition, they showed a time pr ... | 2011 | 21291496 |
| disposable diapers biodegradation by the fungus pleurotus ostreatus. | this research assesses the feasibility of degrading used disposable diapers, an important component (5-15% in weight) of urban solid waste in mexico, by the activity of the fungus pleurotus ostreatus, also known as oyster mushroom. disposable diapers contain polyethylene, polypropylene and a super absorbent polymer. nevertheless, its main component is cellulose, which degrades slowly. p. ostreatus has been utilized extensively to degrade cellulosic materials of agroindustrial sources, using in s ... | 2011 | 21474296 |
| skin test reactivity of allergic subjects to basidiomycetes' crude extracts in a tropical environment. | fungal allergies can be detected by the skin prick test with extracts of the organisms, but not all fungi, including the basidiomycetes, are being examined. we determined the level of sensitization to basidiomycetes in allergic subjects and compared their reactivity to commercial extracts commonly used to detect allergies. crude spore extracts of the basidiomycetes ganoderma applanatum, chlorophyllum molybdites, and pleurotus ostreatus, which are known to release numerous spores, were examined a ... | 2011 | 21506892 |
| anti-inflammatory activity of edible oyster mushroom is mediated through the inhibition of nf-kb and ap-1 signaling. | abstract: background: mushrooms are well recognized for their culinary properties as well as for their potency to enhance immune response. in the present study, we evaluated anti-inflammatory properties of an edible oyster mushroom (pleurotus ostreatus) in vitro and in vivo. methods: raw264.7 murine macrophage cell line and murine splenocytes were incubated with the oyster mushroom concentrate (omc, 0-100 ug/ml) in the absence or presence of lipopolysacharide (lps) or concanavalin a (cona), resp ... | 2011 | 21575254 |
| inhibitory effect of a glycoprotein isolated from golden oyster mushroom (pleurotus citrinopileatus) on the lipopolysaccharide-induced inflammatory reaction in raw 264.7 macrophage. | mushrooms have become an important source of natural antitumor, antiviral, anti-bacterial, immunomodulatory, and anti-inflammatioy agents. golden oyster mushroom, pleurotus citrinopileatus, is a common mushroom in oriental countries for human consumption. the present study was to investigate the anti-inflammatory reaction and immunomodulatory of the bioactive non-lectin glycoprotein (pcp-3a) isolated from the fresh fruiting body of this mushroom. western blot analysis on lps-induced inos, cox-2, ... | 2011 | 21627087 |
| co-cultivation of mutant penicillium oxalicum sau(e)-3.510 and pleurotus ostreatus for simultaneous biosynthesis of xylanase and laccase under solid-state fermentation. | co-cultivation of mutant penicillium oxalicum sau(e)-3.510 and pleurotus ostreatus mtcc 1804 was evaluated for the production of xylanase-laccase mixture under solid-state fermentation (ssf) condition. growth compatibility between mutant p. oxalicum sau(e)-3.510 and white rot fungi (p. ostreatus mtcc 1804, trametes hirsuta mtcc 136 and pycnoporus sp. mtcc 137) was analyzed by growing them on potato dextrose agar plate. extracellular enzyme activities were determined spectrophotometrically. under ... | 2011 | 21642024 |
| co-cultured production of lignin-modifying enzymes with white-rot fungi. | co-cultivation was a potential strategy in lignocellulolytic biodegradation with producing high activity enzymes due to their synergistic action. the objective of this study was to investigate the rarely understood effects of co-culturing of two white-rot fungi on lignin-modifying enzymes (lmes) production. six species, bjerkandera adusta, phlebia radiata, pleurotus ostreatus, dichomitus squalens, hypoxylon fragiforme and pleurotus eryngii, were cultured in pairs to study the production of lmes. ... | 2011 | 21647688 |
| fungal solid state fermentation on agro-industrial wastes for acid wastewater decolorization in a continuous flow packed-bed bioreactor. | this study was aimed at developing a process of solid state fermentation (ssf) with the fungi pleurotus ostreatus and trametes versicolor on apple processing residues for wastewater decolorization. both fungi were able to colonize apple residues without any addition of nutrients, material support or water. p. ostreatus produced the highest levels of laccases (up to 9u g(-1) of dry matter) and xylanases (up to 80u g(-1) of dry matter). a repeated batch decolorization experiment was set up with ap ... | 2011 | 21652205 |
| response to oyster mushroom (pleurotus ostreatus) extract by sensitized and nonsensitized guinea pig trachea. | occupational exposure to oyster mushroom (pleurotus ostreatus) has been associated with obstructive lung disease. previously, we studied an extract of oyster mushroom (ome) and determined that it causes dose-dependent contractions of nonsensitized guinea pig trachea (gpt). we extend these studies to the investigation of sensitized tissue. in the present study 24 animals were sensitized using ovalbumin (oa) and subsequently challenged with an aerosol of 2.5% oa. a control group of 12 nonsensitize ... | 2011 | 21667260 |
| anti-cancer effects of protein extracts from calvatia lilacina, pleurotus ostreatus and volvariella volvacea. | calvatia lilacina (cl), pleurotus ostreatus (po) and volvariella volvacea (vv) are widely distributed worldwide and commonly eaten as mushrooms. in this study, cell viabilities were evaluated for a human colorectal adenocarcinoma cell line (sw480 cells) and a human monocytic leukemia cell line (thp-1 cells). apoptotic mechanisms induced by the protein extracts of po and vv were evaluated for sw480 cells. the viabilities of thp-1 and sw480 cells decreased in a concentration-dependent manner after ... | 2011 | 21792367 |
| antihyperlipidemic effects of pleurotus ostreatus (oyster mushrooms) in hiv-infected individuals taking antiretroviral therapy. | abstract: | 2011 | 21831304 |
| comparative study of antioxidant activities and total phenolic content of selected edible wild mushrooms. | the present study aims to assess the antioxidant activities (aoa) and total phenolic content (tpc) of water extracts of selected edible wild mushrooms: pleurotus porrigens, schizophyllum commune, hygrocybe conica, and lentinus ciliatus. the aoa were evaluated against dpph radical and abts radical cation scavenging ability, ferric-reducing antioxidant power (frap) and beta-carotene-linoleate bleaching (beta-cb) assays, and the folin-ciocalteu method for tpc. bha was used as reference. p. porrigen ... | 2011 | 22135876 |
| Engineered tobacco and microalgae secreting the fungal laccase POXA1b reduce phenol content in olive oil mill wastewater. | Olive oil mill wastewaters (OMWs) are characterised by low pH and a high content of mono- and polyaromatic compounds that exert microbial and phytotoxic activity. The laccase cDNA of the poxA1b gene from Pleurotus ostreatus, carrying a signal peptide sequence for enzyme secretion and driven by the CaMV 35S promoter, was cloned into a plant expression vector. Nuclear genetic transformation was carried out by co-cultivation of Agrobacterium tumefaciens with tobacco cv Samsun NN leaves and cells of ... | 2011 | 22142729 |
| a family gh51 α-l: -arabinofuranosidase from pleurotus ostreatus: identification, recombinant expression and characterization. | an α-l: -arabinofuranosidase produced by pleurotus ostreatus (poabf) during solid state fermentation on tomato pomace was identified and the corresponding gene and cdna were cloned and sequenced. molecular analysis showed that the poabf gene carries 26 exons interrupted by 25 introns and has an open reading frame encoding a protein of 646 amino acid residues, including a signal peptide of 20 amino acid residues. the amino acid sequence similar to the other α-l: -arabinofuranosidases indicated th ... | 2011 | 22080345 |
| Acylhomoserine lactone production by bacteria associated with cultivated mushrooms. | The main bacterial pathogens of cultivated mushroom as well as mushroom-associated bacteria, which were isolated from Agaricus bisporus, Pleurotus ostreatus and Pleurotus eryngii mushroom niches, were evaluated for the production of N-acyl-L-homoserine lactones (AHLs) by using four bioreporters. Furthermore, identification of AHLs by LC-ESI-FTICR MS was performed on culture filtrates of selected pathogens and mushroom-associated bacteria strains, which resulted in inducing at least one of the fo ... | 2011 | 21942309 |
| Antioxidant properties of extracts of wild medicinal mushroom species from Croatia. | Antioxidant activity and total phenol (TP) content of methanol and water extracts of three wild Croatian mushroom species Auricularia auricula-judae (Bull.) Quél., Sarcoscypha austriaca (Sacc.) Boud., and Strobilurus esculentus (Wulfen) Singer were determined and compared with the values obtained for extracts of four cultivated mushrooms Agaricus bisporus (J.E. Lange) Imbach (brown and white strains), Pleurotus ostreatus (Jacq.) P. Kumm., and Lentinus edodes (Berk.) Singer. Spectrophotometric de ... | 2011 | 22135877 |
| chemical composition and nutrition value of dried cultivated culinary-medicinal mushrooms from turkey. | dietary fiber, raw fiber, fructose, glucose, sucrose, maltose, vitamin a (retinol), b1 (thiamine), b2 (riboflavin), b6 (pyridoxine), and niacin contents of dried cultivated mushroom species agaricus bisporus (white and brown), lentinus edodes, and pleurotus ostreatus were determined and evaluated for nutrient and chemical composition. assays of dried mushroom samples were carried out after the drying process. dried shiitake samples showed the highest dietary fiber and raw fiber content (23.23 +/ ... | 2011 | 22164765 |
| biodegradation of chlorobenzoic acids by ligninolytic fungi. | we investigated the abilities of several perspective ligninolytic fungal strains to degrade 12 mono-, di- and trichloro representatives of chlorobenzoic acids (cbas) under model liquid conditions and in contaminated soil. attention was also paid to toxicity changes during the degradation, estimated using two luminescent assay variations with vibrio fischeri. the results show that almost all the fungi were able to efficiently degrade cbas in liquid media, where irpex lacteus, pycnoporus cinnabari ... | 2011 | 21963171 |
| in vitro prevention of cataract by oyster mushroom pleurotus florida extract on isolated goat eye lens. | the aim of the present work was to evaluate the in vitro effect of pleurotus florida extract cataract induced by glucose. | 2011 | 22144771 |
| Chemical composition and nutritional value of the most widely appreciated cultivated mushrooms: An inter-species comparative study. | Herein, it was reported and compared the chemical composition and nutritional value of the most consumed species as fresh cultivated mushrooms: Agaricus bisporus (white and brown mushrooms), Pleurotus ostreatus (oyster mushroom), Pleurotus eryngii (King oyster mushroom), Lentinula edodes (Shiitake) and Flammulina velutipes (Golden needle mushroom). Shiitake revealed the highest levels of macronutrients, unless proteins, as also the highest sugars, tocopherols and PUFA levels, and the lowest SFA ... | 2011 | 22056333 |
| Profiling of hepatic gene expression of mice fed with edible japanese mushrooms by DNA microarray analysis: comparison among Pleurotus ostreatus, Grifola frondosa, and Hypsizigus marmoreus. | To compare and estimate the effects of dietary intake of three kinds of mushrooms (Pleurotus ostreatus, Grifola frondosa, and Hypsizigus marmoreus), mice were fed a diet containing 10-14% of each mushroom for 4 weeks. Triacylglycerol in the liver and plasma decreased and plasma cholesterol increased in the P. ostreatus-fed group compared with those in the control group. Cholesterol in the liver was lower in the G. frondosa-fed group than in the control group, but no changes were found in the H. ... | 2011 | 21910414 |
| Trypanocidal Activity of Ergosterol Peroxide from Pleurotus ostreatus. | Chagas' disease, which is caused by the protozoan parasite Trypanosoma cruzi, is a public health problem in South America affecting millions of people, and more recently several thousands in countries where the disease is not endemic. Due to the magnitude of the problem, finding a cure for this disease remains a major challenge. The aim of this study is to evaluate the trypanocidal activity of ergosterol peroxide (5a, 8a-epidioxy-22E-ergosta-6, 22-dien-3ß-ol) isolated from Pleurotus ostreatus (J ... | 2011 | 22083593 |
| Thermus composti sp. nov., isolated from oyster mushroom compost. | A Gram-negative, rod-shaped bacterium strain (K-39(T)) was isolated from the thermophilic phase of the composting process for oyster mushroom substrate preparation. The strain grew at 40-80 °C (optimum, 65-75 °C), at pH 5-9 (optimum, pH 7), in media containing up to 1.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain K-39(T) formed a distinct lineage within the genus Thermus. Its closest cultivated relative was Thermus islandicus PRI 3838(T) (96.8% similari ... | 2011 | 21856987 |
| indole compounds in some culinary-medicinal higher basidiomycetes from poland. | methanolic extracts of two species collected from natural habitats in poland, boletus edulis and suillus luteus, and one species from a commercial source, pleurotus ostreatus, were analyzed for the presence of non-hallucinogenic indole compounds. the contents of indole compounds in these species were both qualitatively and quantitatively diverse, ranging from 0.01 to 34.11 mg/100 g d.w. two of 11 tested indole compounds, 5-hydroxytryptophan (0.18, 2.08, 1.63 mg/100 g d.w.) and serotonin (6.52, 1 ... | 2011 | 22324410 |
| antitumor effect of culinary-medicinal oyster mushroom, pleurotus ostreatus (jacq.: fr.) p. kumm., derived protein fraction on tumor-bearing mice models. | previously, we reported the in vitro anticancer and immunomodulatory effect of a protein fraction designated as cibacron blue affinity purified protein (cbaep) obtained from the culinary-medicinal oyster mushroom, pleurotus ostreatus. in the present study, we investigated the in vivo antitumor potential of cbaep in different tumor-bearing mice models and studied the detailed mechanism of tumor regression in dalton lymphoma (dl)-bearing mice. the lethal dose (ld50) of cbaep was found to be 55 mg/ ... | 2011 | 22324408 |
| optimization and kinetics evaluation of biodegradation of synthetic azo reactive dye by fungal consortium. | wastewater containing direct dyes discharged from various industries, in particular, textile industry, often cause many environmental problems. among the various effluent treatment methods, biological methods found to be cost effective and do not end up in secondary pollutants. in this study, an attempt has been made to study the decolorization of cibacron yellow s-3r, an azo reactive dye by using fungal cultures such as coriolus versicolor, phanerochaete chrysosporium, pleurotus ostreatus, and ... | 2011 | 23505830 |
| the first report on mushroom green mould disease in croatia. | green mould disease, caused by trichoderma species, is a severe problem for mushroom growers worldwide, including croatia. trichoderma strains were isolated from green mould-affected agaricus bisporus (button or common mushroom) compost and pleurotus ostreatus (oyster mushroom) substrate samples collected from croatian mushroom farms. the causal agents of green mould disease in the oyster mushroom were t. pleurotum and t. pleuroticola, similar to other countries. at the same time, the pathogen o ... | 2012 | 23334043 |
| β-cyclodextrin production by the cyclodextrin glucanotransferase from paenibacillus illinoisensis zy-08: cloning, purification, and properties. | the gene encoding the cyclodextrin glucanotransferase (cgtase, ec2.4.1.19) of paenibacillus illinoisensis was isolated, cloned, sequenced and expressed in escherichia coli. sequence analysis showed that the mature enzyme (684 amino acids) was preceded by a signal peptide of 34-residues. the deduced amino acid sequence of the cgtase from p. illinoisensis zy-08 exhibited highest identity (99 %) to the cgtase sequence from bacillus licheniformis (p14014). the four consensus regions of carbohydrate ... | 2012 | 23264152 |
| induction of a t-helper 1 (th1) immune response in mice by an extract from the pleurotus eryngii (eringi) mushroom. | to assess the effect of edible mushroom extracts on the induction of t-helper 1 (th1) immunity, we examined differences in interferon-gamma (ifn-γ) and interleukin (il)-4 production in mice induced by hot-water extracts of 15 species of edible mushroom. extracts from agaricus bisporus, flammulina velutipes, hypsizigus marmoreus, lentinula edodes, and lyophyllum decastes induced both ifn-γ and il-4 production in mice, whereas extracts from pleurotus ostreatus only induced il-4. in contrast, extra ... | 2012 | 23134464 |
| aflatoxin detoxification by manganese peroxidase purified from pleurotus ostreatus. | manganese peroxidase (mnp) was produced from white rot edible mushroom pleurotus ostreatus on the culture filtrate. the enzyme was purified to homogeneity using (nh4)2so4 precipitation, deae-sepharose and sephadex g-100 column chromatography. the final enzyme activity achieved 81 u ml(-1), specific activity 78 u mg(-1) with purification fold of 130 and recovery 1.2% of the crude enzyme. sds-page indicated that the pure enzyme have a molecular mass of approximately 42 kda. the optimum ph was betw ... | 2014 | 24948923 |
| biological removal of azo and triphenylmethane dyes and toxicity of process by-products. | increasing environmental pollution is connected with broad applications of dyes and imperfection of dyeing technology. decolourization of triphenylmethane brilliant green and disazo evans blue by bacterial and fungal strains and toxicity (phyto- and zootoxicity) of degradation by-products were investigated. influence of incubation method on dyes removal was evaluated (static, semi-static, shaken). dead biomass was used for sorption estimation. toxicity of treated dyes was measured to estimate po ... | 2011 | 22593606 |
| abiotic and biotic degradation of oxo-biodegradable plastic bags by pleurotus ostreatus. | in this study, we evaluated the growth of pleurotus ostreatus plo6 using oxo-biodegradable plastics as a carbon and energy source. oxo-biodegradable polymers contain pro-oxidants that accelerate their physical and biological degradation. these polymers were developed to decrease the accumulation of plastic waste in landfills. to study the degradation of the plastic polymers, oxo-biodegradable plastic bags were exposed to sunlight for up to 120 days, and fragments of these bags were used as subst ... | 2014 | 25419675 |
| description of the first fungal dye-decolorizing peroxidase oxidizing manganese(ii). | two phylogenetically divergent genes of the new family of dye-decolorizing peroxidases (dyps) were found during comparison of the four dyp genes identified in the pleurotus ostreatus genome with over 200 dyp genes from other basidiomycete genomes. the heterologously expressed enzymes (pleos-dyp1 and pleos-dyp4, following the genome nomenclature) efficiently oxidize anthraquinoid dyes (such as reactive blue 19), which are characteristic dyp substrates, as well as low redox-potential dyes (such as ... | 2015 | 25967658 |
| effect of casing layer on growth promotion of the edible mushroom pleurotus ostreatus. | various bacteria were isolated from the casing layer soil of the culture bed of p. ostreatus and their role in fruiting body induction of the edible mushroom, p. ostreatus, was investigated. analysis of the bacterial community isolated from the casing layer soil revealed that the composition of genera and number of cultivable bacteria were different for each sterilizing treatment. bordetella was predominant in the bulk soil whereas flavobacterium was predominant after sterilization of the casing ... | 2008 | 23997606 |
| photo-irradiated biosynthesis of silver nanoparticles using edible mushroom pleurotus florida and their antibacterial activity studies. | this is a report on photo-irradiated extracellular synthesis of silver nanoparticles using the aqueous extract of edible oyster mushroom (pleurotus florida) as a reducing agent. the appearance, size, and shape of the silver nanoparticles are understood by uv-visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and atomic force microscopy. the x-ray diffraction studies, energy dispersive x-ray analysis indicate that particles are crystalline in natu ... | 2011 | 22190895 |
| crystallization and preliminary x-ray crystallographic analysis of the small subunit of the heterodimeric laccase poxa3b from pleurotus ostreatus. | laccases are multicopper oxidases of great biotechnological potential. while laccases are generally monomeric glycoproteins, the white-rot fungus pleurotus ostreatus produces two closely related heterodimeric isoenzymes composed of a large subunit, homologous to the other fungal laccases, and a small subunit. the sequence of the small subunit does not show significant homology to any other protein or domain of known function and consequently its function is unknown. the highest similarity to pro ... | 2013 | 24419623 |
| genetic linkage map of the edible basidiomycete pleurotus ostreatus. | we have constructed a genetic linkage map of the edible basidiomycete pleurotus ostreatus (var. florida). the map is based on the segregation of 178 random amplified polymorphic dna and 23 restriction fragment length polymorphism markers; four hydrophobin, two laccase, and two manganese peroxidase genes; both mating type loci; one isozyme locus (est1); the rrna gene sequence; and a repetitive dna sequence in a population of 80 sibling monokaryons. the map identifies 11 linkage groups correspondi ... | 0 | 11097904 |
| genomic treasure troves: complete genome sequencing of herbarium and insect museum specimens. | unlocking the vast genomic diversity stored in natural history collections would create unprecedented opportunities for genome-scale evolutionary, phylogenetic, domestication and population genomic studies. many researchers have been discouraged from using historical specimens in molecular studies because of both generally limited success of dna extraction and the challenges associated with pcr-amplifying highly degraded dna. in today's next-generation sequencing (ngs) world, opportunities and p ... | 2013 | 23922691 |
| release of pleurotus ostreatus versatile-peroxidase from mn2+ repression enhances anthropogenic and natural substrate degradation. | the versatile-peroxidase (vp) encoded by mnp4 is one of the nine members of the manganese-peroxidase (mnp) gene family that constitutes part of the ligninolytic system of the white-rot basidiomycete pleurotus ostreatus (oyster mushroom). vp enzymes exhibit dual activity on a wide range of substrates. as mn(2+) supplement to p. ostreatus cultures results in enhanced degradation of recalcitrant compounds and lignin, we examined the effect of mn(2+) on the expression profile of the mnp gene family. ... | 2012 | 23285046 |
| characterization of the highly active fragment of glyceraldehyde-3-phosphate dehydrogenase gene promoter for recombinant protein expression in pleurotus ostreatus. | developing efficient native promoters is important for improving recombinant protein expression by fungal genetic engineering. the promoter region of glyceraldehyde-3-phosphate dehydrogenase gene in pleurotus ostreatus (pogpd) was isolated and optimized by upstream truncation. the activities of these promoters with different lengths were further confirmed by fluorescence, quantitative real-time pcr and western blot analysis. a truncated pogpd-p2 fragment (795 bp) drove enhanced green fluorescenc ... | 2015 | 25743073 |
| biodegradation and detoxification of olive mill wastewater by selected strains of the mushroom genera ganoderma and pleurotus. | thirty-nine white-rot fungi belonging to nine species of agaricomycotina (basidiomycota) were initially screened for their ability to decrease olive-mill wastewater (omw) phenolics. four strains of ganoderma australe, ganoderma carnosum, pleurotus eryngii and pleurotus ostreatus, were selected and further examined for key-aspects of the omw biodegradation process. fungal growth in omw-containing batch cultures resulted in significant decolorization (by 40-46% and 60-65% for ganoderma and pleurot ... | 2012 | 22480939 |
| aflatoxin b1 degradation during co-cultivation of aspergillus flavus and pleurotus ostreatus strains on rice straw. | aflatoxin b1 (afb1) produced by aspergillus flavus is known to have carcinogenic and teratogenic effects on animal health. accidental feeding of afb1-contaminated rice straw may be detrimental to dairy cattle. white-rot basidiomycetous fungus pleurotus ostreatus can grow on different agronomic wastes by synthesizing different ligninolytic enzymes. these extracellular enzymes are capable of degrading many environmentally hazardous compounds including afb1. the present study examines the ability o ... | 2015 | 28324293 |
| antioxidant and antifungal potential of pleurotus ostreatus and agrocybe cylindracea basidiocarps and mycelia. | basidiocarps of pleurotus ostreatus and agrocybe cylindracea are characterized by high nutritional value and numerous medicinal activities, though bioactivities of their mycelia have not been sufficiently studied. the aim of the study was to evaluate antioxidant and antifungal potentials of p. ostreatus and a. cylindracea basidiocarp extracts, as well as those obtained from mycelia cultivated in common synthetic medium and media enriched with various agro-industrial residues. the free radical sc ... | 2015 | 25483715 |
| screening of indigenously isolated fungi for lovastatin production and its in vivo evaluation. | seven indigenously isolated fungal strains (aspergillus niger, aspergillus flavus, aspergillus terreus, penicillium citrinum, penicillium notatum, pleurotus ostreatus and trichoderma viradae) were tested for their potential to produce cholesterol lowering drug lovastatin by using different agro-industrial wastes (corn cobs, corn stover, banana stalk, wheat straw, wheat bran, bagasse) in submerged as well as solid state fermentation. aspergillus terreus showed maximum production of 18.74 mg/100 m ... | 2014 | 24894549 |
| β-glucan synthase gene overexpression and β-glucans overproduction in pleurotus ostreatus using promoter swapping. | mushroom β-glucans are potent immunological stimulators in medicine, but their productivities are very low. in this study, we successfully improved its production by promoter engineering in pleurotus ostreatus. the promoter for β-1,3-glucan synthase gene (gls) was replaced by the promoter of glyceraldehyde-3-phosphate dehydrogenase gene of aspergillus nidulans. the homologous recombination fragment for swapping gls promoter comprised five segments, which were fused by two rounds of combined touc ... | 2013 | 23637884 |
| water recycle as a must: decolorization of textile wastewaters by plant-associated fungi. | textile dye effluents are among the most problematic pollutants because of their toxicity on several organisms and ecosystems. low cost and ecocompatible bioremediation processes offer a promising alternative to the conventional and aspecific physico-chemical procedures adopted so far. here, microorganisms resident on three real textile dyeing effluent were isolated, characterized, and tested for their decolorizing performances. although able to survive on these real textile-dyeing wastewaters, ... | 2014 | 23553673 |
| influence of structure of lactones with the methylcyclohexene and dimethylcyclohexene ring on their biotransformation and antimicrobial activity. | the aim of this article is influence of the structure of lactones with the methylcyclohexene and dimethylcyclohexene ring on their biotransformation and antimicrobial activity. this work was based on the general remark that even the smallest change in the structure of a compound can affect its biological properties. the results of the biotransformation of four bicyclic unsaturated lactones with one or two methyl groups in the cyclohexene ring was tested using fifteen fungal strains (fusarium spe ... | 2017 | 28107178 |
| a possible water-soluble inducer for synthesis of cellulase in aspergillus niger. | the synthesis of cellulase in filamentous fungi can be triggered by several inducers. in this study, a bamboo-shoot shell pretreated with pleurotus ostreatus could promote the formation of cellulases in aspergillus niger. further identification, including uplc-tof-ms, ultrafiltration, and ft-ir, denoted that the soluble inducer was not a traditional disaccharide but a type of modified lignin polymer. this revelation may result in incipient strategies to ameliorate cellulase productivity. | 2017 | 27993445 |
| fungal biodegradation of anthracene-polluted cork: a comparative study. | the efficiency of cork waste in adsorbing aqueous polycyclic aromatic hydrocarbons (pahs) has been previously reported. biodegradation of contaminated cork using filamentous fungi could be a good alternative for detoxifying cork to facilitate its final processing. for this purpose, the degradation efficiency of anthracene by three ligninolytic white-rot fungi (phanerochaete chrysosporium, irpex lacteus and pleurotus ostreatus) and three non-ligninolytic fungi which are found in the cork itself ( ... | 2016 | 26540209 |
| benzoic acid derivatives with improved antifungal activity: design, synthesis, structure-activity relationship (sar) and cyp53 docking studies. | previously, we identified cyp53 as a fungal-specific target of natural phenolic antifungal compounds and discovered several inhibitors with antifungal properties. in this study, we performed similarity-based virtual screening and synthesis to obtain benzoic acid-derived compounds and assessed their antifungal activity against cochliobolus lunatus, aspergillus niger and pleurotus ostreatus. in addition, we generated structural models of cyp53 enzyme and used them in docking trials with 40 selecte ... | 2015 | 26154240 |
| effective mutations in a high redox potential laccase from pleurotus ostreatus. | since the first report on a laccase, there has been a notable development in the interest towards this class of enzymes, highlighted from the number of scientific papers and patents about them. at the same time, interest in exploiting laccases-mainly high redox potential-for various functions has been growing exponentially over the last 10 years. despite decades of work, the molecular determinants of the redox potential are far to be fully understood. for this reason, interest in tuning laccase ... | 2014 | 24463760 |
| antifungal activity of cinnamic acid derivatives involves inhibition of benzoate 4-hydroxylase (cyp53). | cyp53a15, from the sorghum pathogen cochliobolus lunatus, is involved in detoxification of benzoate, a key intermediate in aromatic compound metabolism in fungi. because this enzyme is unique to fungi, it is a promising drug target in fungal pathogens of other eukaryotes. | 2014 | 24314266 |
| screening of medicinal higher basidiomycetes mushrooms from turkey for lovastatin production. | as a first attempt, a study was carried out to test for lovastatin production ability in local higher basidiomycetes mushroom isolates from turkey. an extended screening was performed for lovastatin production in yeast lactose agar medium, among a total of 136 macrofungi isolates from the basidiomycetes culture collection of eskisehir osmangazi university. lovastatin production was evaluated by disc diffusion method and was also confirmed by tlc and hplc. only six isolates were found to be lovas ... | 2012 | 22506575 |
| antioxidant activity via dpph, gram-positive and gram-negative antimicrobial potential in edible mushrooms. | edible mushrooms (ems) are nutritionally rich source of proteins and essential amino acids. in the present study, the antioxidant activity via 1,1-diphenyl-2-picrylhydrazyl (dpph) and antimicrobial potential in ems (pleurotus ostreatus, morchella esculenta, p. ostreatus (black), p. ostreatus (yellow) and pleurotus sajor-caju) were investigated. the dpph radical scavenging activity revealed that the significantly higher activity (66.47%) was observed in morchella esculenta at a maximum concentrat ... | 2014 | 23095488 |
| effect of bacteria on the degradation ability of pleurotus ostreatus. | white-rot fungi are efficient degraders of lignin whose extracellular enzymes have a potential to degrade organopollutants. in natural conditions these fungi enter into interactions with other organisms, which may affect their biodegradation capacity. the aim was to investigate the ability of pleurotus ostreatus to form stable biofilms and to test the capacity of the fungus to degrade remazol brilliant blue r in mixed cultures with bacteria. bacterial counts were determined to see the behavior o ... | 2017 | 28187935 |
| study of antimicrobial and cytotoxic potential of the oyster mushroom pleurotus ostreatus cv. florida (agaricomycetes). | this work was undertaken to evaluate in vitro antimicrobial and cytotoxic potential of pleurotus ostreatus cv. florida. mushroom basidiocarps were extracted in water:ethanol (1:1, v/v), and the resulting extract was subjected to antimicrobial studies against escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, klebsiella oxytoca, bacillus subtilis, and candida albicans. cytotoxic potential on viable human leukocytes was studied. in vitro results showed excellent antimicrobial and cyt ... | 2016 | 27481298 |
| antimicrobial activity of extracts of the oyster culinary medicinal mushroom pleurotus ostreatus (higher basidiomycetes) and identification of a new antimicrobial compound. | pleurotus ostreatus is an edible mushroom that also has high medicinal values. in this study, p. ostreatus was tested for its ability to inhibit the growth of fungi and bacteria. the freeze-dried fruiting body, broth from submerged culture, and mycelial biomass of p. ostreatus were extracted using alcohols and water as solvents. the extracts were then tested for their antimicrobial activity against the growth of fungi and bacteria. it was observed that the water extract from fruiting bodies had ... | 2015 | 26349515 |
| biosynthesis and characterization of silver nanoparticles produced by pleurotus ostreatus and their anticandidal and anticancer activities. | the biosynthesis of nanoparticles has received increasing interest because of the growing need to develop safe, cost-effective and environmentally friendly technologies for the synthesis of nano-materials. in this study, silver nanoparticles (agnps) were synthesized using a reduction of aqueous ag(+) ions with culture supernatant from pleurotus ostreatus. the bioreduction of agnps was monitored by ultra violet-visible spectroscopy and the obtained agnps were characterized by transmission electro ... | 2014 | 25053172 |
| isolation of fungal pathogens to an edible mushroom, pleurotus eryngii, and development of specific its primers. | fungal pathogens have caused severe damage to the commercial production of pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. four strains of pathogenic fungi, including trichoderma koningiopsis dc3, phomopsis sp. mp4, mucor circinelloides mp5, and cladosporium bruhnei mp6, were isolated from the bottle culture of diseased p. eryngii. a species-specific primer set was designed for each fungus from the i ... | 2013 | 24493949 |
| the sensing of membrane microdomains based on pore-forming toxins. | membrane rafts are transient and unstable membrane microdomains that are enriched in sphingolipids, cholesterol, and specific proteins. they are involved in intracellular trafficking, signal transduction, pathogen entry, and attachment of various ligands. increasing experimental evidence on the crucial biological roles of membrane rafts under normal and pathological conditions require new techniques for their structural and functional characterization. in particular, fluorescence-labeled cytolyt ... | 2013 | 23244522 |
| comparison of the interleukin-1β-inducing potency of allergenic spores from higher fungi (basidiomycetes) in a cryopreserved human whole blood system. | spores from basidiomycete fungi (basidiospores) are highly prevalent in the atmosphere of urban and rural settings. studies have confirmed their potential to affect human health as allergens. less is known about their potential to serve as stimuli of the innate immune system and induce proinflammatory reactions. | 2014 | 24356469 |
| analysis of synonymous codon usage patterns in the edible fungus volvariella volvacea. | in this study, trends in synonymous codons usage of volvariella volvecea have been first examined by analysis of complete coding sequences and gene chip data. the results showed that gc content at three codon positions are obviously different and there were several factors shaping the codon usage of v. volvacea genes, including base composition. the comparison of codon usage among four edible fungi such as v. volvacea, agaricus bisporus, coprinopsis cinerea, and pleurotus ostreatus indicated tha ... | 2016 | 27696508 |
| an aryl-alcohol oxidase of pleurotus sapidus: heterologous expression, characterization, and application in a 2-enzyme system. | aryl-alcohol oxidases (aaos) are enzymes supporting the degradation of lignin by fungal derived class ii peroxidases produced by white-rot fungi. aaos are able to generate h2o2 as a by-product via oxidation of an aryl-alcohol into its correspondent aldehyde. in this study, an aao was heterologously expressed in a basidiomycete host for the first time. the gene for an aao of the white-rot fungus pleurotus sapidus, a close relative to the oyster mushroom pleurotus ostreatus, was cloned into an exp ... | 2016 | 27138199 |
| absence of a gene encoding cytosine deaminase in the genome of the agaricomycete coprinopsis cinerea enables simple marker recycling through 5-fluorocytosine counterselection. | coprinopsis cinerea is a model species for molecular genetics studies of sexual development in agaricomycetes or homobasidiomycetes. recently, efficient gene targeting was established in this fungus by generating cc.ku70 or cc.lig4 disruptants. to determine the molecular mechanisms underlying sexual development, which involves many genes, generating multiple gene disruptants is required. however, the number of transformation markers available for c. cinerea is limited. this problem would be solv ... | 2015 | 26223587 |
| [codon usage bias in the straw mushroom volvariella volvacea]. | we analyzed the whole genome coding sequence of volvariella volvacea to study the pattern utilization of codons by codon w 1.4.2. as results, 24 optimal codons were identified. moreover, the frequency of codons usage was calculated by cusp program. we compared the frequency of codons usage of v. volvacea with other organisms including 6 modal value species (homo sapiens, saccharomys cerevisiae, arabidopsis thalian, mus musculus, danio rerio and drosophila melanogaster) and 4 edible fungi (coprin ... | 2014 | 25720157 |
| microsatellites in the genome of the edible mushroom, volvariella volvacea. | using bioinformatics software and database, we have characterized the microsatellite pattern in the v. volvacea genome and compared it with microsatellite patterns found in the genomes of four other edible fungi: coprinopsis cinerea, schizophyllum commune, agaricus bisporus, and pleurotus ostreatus. a total of 1346 microsatellites have been identified, with mono-nucleotides being the most frequent motif. the relative abundance of microsatellites was lower in coding regions with 21 no./mb. howeve ... | 2014 | 24575404 |
| the amoebicidal effect of ergosterol peroxide isolated from pleurotus ostreatus. | dysentery is an inflammation of the intestine caused by the protozoan parasite entamoeba histolytica and is a recurrent health problem affecting millions of people worldwide. because of the magnitude of this disease, finding novel strategies for treatment that does not affect human cells is necessary. ergosterol peroxide is a sterol particularly known as a major cytotoxic agent with a wide spectrum of biological activities produced by edible and medicinal mushrooms. the aim of this report is to ... | 2015 | 26392373 |
| transformation and toxicity evaluation of tetracycline in humic acid solution by laccase coupled with 1-hydroxybenzotriazole. | enzyme-based catalyzed oxidative coupling reactions (e-cocrs) are considered as viable technologies to transform a variety of pharmaceutical antibiotics. this study indicated that the extracellular fungal laccase from pleurotus ostreatus was effective in transforming tetracycline (tc) with 1-hydroxybenzotriazole (hbt) present at varying conditions during e-cocrs. the presence of humic acid (ha) showed suppressive effect on the transformation rate constants (k) of tc, and the k values for tc decr ... | 2017 | 28273567 |
| antimicrobial coumarins from the oyster culinary-medicinal mushroom, pleurotus ostreatus (agaricomycetes), from kenya. | pleurotus ostreatus has been widely used as food because of its nutritional and medicinal properties. these have been attributed to the presence of macronutrients, minerals, vitamins, and amino acids, among other secondary metabolites. there are, however, few reports on the antimicrobial activities of different classes of purified compounds from p. ostreatus. this led to the current study, the objective of which was to chemically characterize the antibiotic activities of p. ostreatus against sel ... | 2016 | 27910758 |
| vmh2 hydrophobin as a tool for the development of "self-immobilizing" enzymes for biosensing. | self-assembling proteins forming amyloid fibrils are promising candidates for the fabrication of biomaterials, due to the chemical and mechanical stability of their structures. among potential applications, their use as platforms for enzyme immobilization is rapidly gathering attention. in this work, we demonstrate that the production of the enzyme glutathione-s-transferase (gst) fused to the class i hydrophobin vmh2 from pleurotus ostreatus represents an invaluable tool for the development of s ... | 2017 | 27427236 |
| comparative cold shock expression and characterization of fungal dye-decolorizing peroxidases. | dye-decolorizing peroxidases (dyps) from auricularia auricula-judae, bjerkandera adusta, pleurotus ostreatus and marasmius scorodonius (basidiomycota) were expressed in escherichia coli using the cold shock-inducible expression system pcold i dna. functional expression was achieved without the addition of hemin or the co-expression of any chaperones. the presence or absence of the native signal sequence had a strong impact on the success of the expression, but the effect was not consistent for t ... | 2016 | 27106285 |