Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| screening of indigenously isolated fungi for lovastatin production and its in vivo evaluation. | seven indigenously isolated fungal strains (aspergillus niger, aspergillus flavus, aspergillus terreus, penicillium citrinum, penicillium notatum, pleurotus ostreatus and trichoderma viradae) were tested for their potential to produce cholesterol lowering drug lovastatin by using different agro-industrial wastes (corn cobs, corn stover, banana stalk, wheat straw, wheat bran, bagasse) in submerged as well as solid state fermentation. aspergillus terreus showed maximum production of 18.74 mg/100 m ... | 2014 | 24894549 |
| effective mutations in a high redox potential laccase from pleurotus ostreatus. | since the first report on a laccase, there has been a notable development in the interest towards this class of enzymes, highlighted from the number of scientific papers and patents about them. at the same time, interest in exploiting laccases-mainly high redox potential-for various functions has been growing exponentially over the last 10 years. despite decades of work, the molecular determinants of the redox potential are far to be fully understood. for this reason, interest in tuning laccase ... | 2014 | 24463760 |
| antifungal activity of cinnamic acid derivatives involves inhibition of benzoate 4-hydroxylase (cyp53). | cyp53a15, from the sorghum pathogen cochliobolus lunatus, is involved in detoxification of benzoate, a key intermediate in aromatic compound metabolism in fungi. because this enzyme is unique to fungi, it is a promising drug target in fungal pathogens of other eukaryotes. | 2014 | 24314266 |
| antiviral activity of basidiomycete mycelia against influenza type a (serotype h1n1) and herpes simplex virus type 2 in cell culture. | in this study, we investigated the in vitro antiviral activity of the mycelia of higher mushrooms against influenza virus type a (serotype h1n1) and herpes simplex virus type 2 (hsv-2), strain bh. all 10 investigated mushroom species inhibited the reproduction of influenza virus strain a/fm/1/47 (h1n1) in mdck cells reducing the infectious titer by 2.0-6.0 lg id50. four species, pleurotus ostreatus, fomes fomentarius, auriporia aurea, and trametes versicolor, were also determined to be effective ... | 2014 | 25358999 |
| chemical composition and nutritional and medicinal value of fruit bodies and submerged cultured mycelia of culinary-medicinal higher basidiomycetes mushrooms. | this research gives the results of a proximate analysis (moisture, ash, crude protein, fat, total carbohydrates, and total energy); a bioactive compounds analysis (γ-aminobutyric acid [gaba], ergothioneine, lovastatin, and cordycepin); fatty acid and amino acid analysis; and an analysis of macro- and microelement content of fruit bodies and mycelia of 15 higher basidiomycetes medicinal mushroom strains belonging to 12 species. the results obtained demonstrate that almost all investigated mushroo ... | 2014 | 24941169 |
| nutrient digestibility, ruminal fermentation activities, serum parameters and milk production and composition of lactating goats fed diets containing rice straw treated with pleurotus ostreatus. | the study evaluated replacement of egyptian berseem clover (bc, trifolium alexandrinum) with spent rice straw (srs) of pleurotus ostreatus basidiomycete in diets of lactating baladi goats. nine lactating homo-parity baladi goats (average bw 23.8±0.4 kg) at 7 d postpartum were used in a triplicate 3×3 latin square design with 30 d experimental periods. goats were fed a basal diet containing 0 (control), 0.25 (srs25) and 0.45 (srs45) (w/w, dm basis) of srs. the control diet was berseem clover and ... | 2014 | 25049962 |
| development of bioprocess for the production of laccase by pleurotus ostreatus mtcc 1802 using evolutionary optimization technique. | for cost effective production of laccase enzyme (benzenediol: oxygen oxidoreductase) from p. ostreatus mtcc 1802 through solid sate fermentation, physico-chemical parameters such as temperature (20-35 degrees c), incubation period (9-17 days) and substrate (neem bark and wheat bran, in various ratios, w/w) were optimized first by one parameter at time approach and then obtained optimum conditions were considered as zero level in evolutionary optimization factorial design technique. at statistica ... | 2014 | 25434106 |
| biodegradation of aflatoxin b1 in contaminated rice straw by pleurotus ostreatus mtcc 142 and pleurotus ostreatus ghbbf10 in the presence of metal salts and surfactants. | aflatoxin b1 (afb1) is a highly toxic fungal metabolite having carcinogenic, mutagenic and teratogenic effects on human and animal health. accidental feeding of aflatoxin-contaminated rice straw may be detrimental for ruminant livestock and can lead to transmission of this toxin or its metabolites into the milk of dairy cattle. white-rot basidiomycetous fungus pleurotus ostreatus produces ligninolytic enzymes like laccase and manganese peroxidase (mnp). these extracellular enzymes have been repo ... | 2014 | 24770873 |
| evaluation of the cytotoxic effects of cam therapies: an in vitro study in normal kidney cell lines. | the purpose of this current study was to justify the incorporation of complementary and alternate medicine (cam) in current cancer treatments. the major drawback of anticancer drugs is their nonselective killing, which ultimately leads to attrition of normal cells. keeping this as the foundation of our study, we made an effort to compare the cytotoxicity associated with a known chemotherapeutic drug 5-fluorouracil (5-fu), with certain cam therapies previously reported to have anticancer activity ... | 2014 | 24672319 |
| characterisation of potential antidiabetic-related proteins from pleurotus pulmonarius (fr.) quél. (grey oyster mushroom) by maldi-tof/tof mass spectrometry. | pleurotus pulmonarius has been reported to have a potent remedial effect on diabetic property and considered to be an alternative for type 2 diabetes mellitus treatment. this study aimed to investigate the antidiabetic properties of ammonium sulphate precipitated protein fractions from p. pulmonarius basidiocarps. preliminary results demonstrated that 30% (nh4)2so4 precipitated fraction (f30) inhibited saccharomyces cerevisiae α-glucosidase activity (24.18%), and 100% (nh4)2so4 precipitated frac ... | 2014 | 25243114 |
| medicinal and antimicrobial role of the oyster culinary-medicinal mushroom pleurotus ostreatus (higher basidiomycetes) cultivated on banana agrowastes in india. | oyster mushrooms, species of the genus pleurotus, are recognized for producing secondary metabolites with important medicinal properties. investigations were carried out to evaluate the antioxidative and antimicrobial properties of the edible mushroom pleurotus ostreatus (mtcc142) extracts cultivated on banana agrowastes. ethanolic extracts showed antimicrobial activities against gram-positive and gram-negative bacteria, and their in vitro antifungal activities against all fungi tested revealed ... | 2014 | 24941164 |
| important nutritional constituents, flavour components, antioxidant and antibacterial properties of pleurotus sajor-caju. | oyster mushroom (pleurotus sajor-caju) cultivated in the laboratory was studied for nutritional constituents, flavor components, antioxidant and antibacterial properties. nutritional constituents estimated per 100 g dry weight (d.w.) include protein (29.3 g), carbohydrate (62.97 g), crude fat (0.91 g), ash (6.82 g) and crude fiber (12.3 g). energy value of this mushroom was about 297.5 kcal/100 g d.w. major mineral components estimated include ca, fe, and mg with highest level of 505.0, 109.5 an ... | 2014 | 25114338 |
| over-expression of psgpd, a mushroom glyceraldehyde-3-phosphate dehydrogenase gene, enhances salt tolerance in rice plants. | transgenic potatoes expressing glyceraldehyde-3-phosphate dehydrogenase (gpd), isolated from the oyster mushroom, pleurotus sajor-caju, had increased tolerance to salt stress (jeong et al. biochem biophys res commun 278:192-196, 2000). to examine the physiological mechanisms enhancing salt tolerance in gpd-transgenic rice plants, the salt tolerance of five gpd transgenic rice lines (t1-t5) derived from dongjin rice cultivar were evaluated in a fixed 150 mm saline environment in comparison to two ... | 2014 | 24737077 |
| box-behnken methodology for cr (vi) and leather dyes removal by an eco-friendly biosorbent: f. vesiculosus. | this study focused on leather industrial effluents treatment by biosorption using fucus vesiculosus as low-cost adsorbent. these effluents are yellowish-brown color and high concentration of cr (vi). therefore, biosorption process was optimized using response surface methodology based on box-behnken design operating with a simulated leather effluent obtained by mixture of cr (vi) solution and four leather dyes. the key variables selected were initial solution ph, biomass dosage and cacl2 concent ... | 2014 | 24484851 |
| assessing the effect of composting cassava peel based substrates on the yield, nutritional quality, and physical characteristics of pleurotus ostreatus (jacq. ex fr.) kummer. | cassava peel based substrate formulations as an alternative substrate were used to grow mushrooms. the effect of two compost heights, three composting periods on the mycelia growth, physical characteristics, yield, and nutritional qualities of pleurotus ostreatus (jacq. ex fr.) kummer was studied. mean mycelia growth of 16.2 cm after a period of seven (7) weeks was the best for 1.5 m compost height. cap diameter and stipe length differed significantly (p < 0.05) with the compost heights (0.8 m a ... | 2014 | 25580299 |
| potential of fungal co-culturing for accelerated biodegradation of petroleum hydrocarbons in soil. | the potential of fungal co-culture of the filamentous pestalotiopsis sp. ng007 with four different basidiomycetes--trametes versicolor u97, pleurotus ostreatus pl1, cerena sp. f0607, and polyporus sp. s133--for accelerating biodegradation of petroleum hydrocarbons (phcs) was studied using three different physicochemical characteristic phcs in soil. all the combinations showed a mutual intermingling mycelial interaction on the agar plates. however, only ng007/s133 (50/50) exhibited an optimum gro ... | 2014 | 24997261 |
| efficiency of treatments for controlling trichoderma spp during spawning in cultivation of lignicolous mushrooms. | trichoderma spp is the cause of the green mold disease in mushroom cultivation production. many disinfection treatments are commonly applied to lignocellulose substrates to prevent contamination. mushroom growers are usually worried about the contaminations that may occur after these treatments during handling or spawning. the aim of this paper is to estimate the growth of the green mold trichoderma sp on lignocellulose substrates after different disinfection treatments to know which of them is ... | 2014 | 25763030 |
| bioconversion of lignocellulosic residues by agrocybe cylindracea and pleurotus ostreatus mushroom fungi--assessment of their effect on the final product and spent substrate properties. | nine agro-industrial and forestry by-products were subjected to solid-state fermentation by agrocybe cylindracea and pleurotus ostreatus, and the process and end-products were comparatively evaluated. grape marc waste plus cotton gin trash was the best performing medium for both fungi, while substrate composition had a marked effect on most cultivation parameters. biological efficiency was positively correlated with nitrogen, lignin and ash, and negatively with hemicelluloses and carbohydrate co ... | 2014 | 24837930 |
| immobilization of defined laccase combinations for enhanced oxidation of phenolic contaminants. | immobilization is an important method to increase enzyme stability and allow enzyme reuse. one interesting application in the field of environmental biotechnology is the immobilization of laccase to eliminate phenolic contaminants via oxidation. fumed silica nanoparticles have interesting potential as support material for laccase immobilization via sorption-assisted immobilization in the perspective of applications such as the elimination of micropollutants in aqueous phases. based on these fact ... | 2014 | 23812279 |
| anti-inflammatory effects of five commercially available mushroom species determined in lipopolysaccharide and interferon-γ activated murine macrophages. | inflammation is a well-known contributing factor to many age-related chronic diseases. one of the possible strategies to suppress inflammation is the employment of functional foods with anti-inflammatory properties. edible mushrooms are attracting more and more attention as functional foods since they are rich in bioactive compounds, but their anti-inflammatory properties and the effect of food processing steps on this activity has not been systematically investigated. in the present study, whit ... | 2014 | 24262531 |
| evaluation of the chemical and antioxidant properties of wild and cultivated mushrooms of ghana. | knowledge of the chemical composition of both wild and cultivated edible mushrooms in ghana is limited. this study reports their nutritional value, composition in lipophilic and hydrophilic molecules, minerals and antioxidant properties. the samples were found to be nutritionally rich in carbohydrates, ranging from 64.14 ± 0.93 g in pleurotus ostreatus strain em-1 to 80.17 ± 0.34 g in lentinus squarosullus strain lsf. the highest level of proteins (28.40 ± 0.86 g) was recorded in the mentioned p ... | 2014 | 25432007 |
| release of feruloylated oligosaccharides from wheat bran through submerged fermentation by edible mushrooms. | wheat bran, a by-product of the flour industry, is believed to be a raw material for the production of feruloylated oligosaccharides (fos) because of its high content of conjiont ferulic acid (fa). studies were carried out to identify edible mushrooms that are able to release fos from wheat bran. all the six tested mushrooms (pleurotus ostreatus, hericium erinaceum, auricularia auricula, cordyceps militaris, agrocybe chaxingu, and ganoderma lucium) were found to release fos, and agrocybe chaxing ... | 2014 | 24123163 |
| biodegradation of naphthalenesulphonate polymers: the potential of a combined application of fungi and bacteria. | the potential of several fungi and their synergy with bacterial biomasses were evaluated as a solution for the removal of 2-naphthalensulphonic acid polymers (2-nsaps) from petrochemical wastewater, characterized by a chemical oxygen demand (cod) greater than 9000 mg/l. the ability of fungi to grow on 2-nsap mixtures was preliminarily investigated using a solid medium, and then the action of the selected strains, both in suspended and immobilized form, was evaluated in terms of degradation, depo ... | 2014 | 25351603 |
| storage temperature and uv-irradiation influence on the ergosterol content in edible mushrooms. | ergosterol (5,7,22-ergostatrien-3β-ol) and ergosteryl derivatives from different genera of edible mushrooms were separated and quantified by an isocratic reversed-phase high performance liquid chromatography (hplc) method. the technique allowed a rapid separation of free ergosterol and two ergosteryl derivatives occurring in mushrooms. the ergosterol content varied considerably depending on the fungus. thus, the species agaricus bisporus and hygrophorus marzuolus presented high quantities of erg ... | 2014 | 24206714 |
| purification and characterization of a novel fibrinolytic enzyme from culture supernatant of pleurotus ostreatus. | a fibrinolytic enzyme was produced by an edible mushroom of pleurotus ostreatus using submerged culture fermentation. the enzyme was purified from the culture supernatant by applying a combination of freeze-thaw treatment, ammonium sulfate precipitation, hydrophobic interaction, and gel filtration chromatographies. the enzyme was purified by a 147-fold, with a yield of 7.54%. the molecular masses of the enzyme an determined by gel filtration and sdspage were 13.6 and 18.2 kda, respectively. the ... | 2014 | 24248089 |
| market survey and risk assessment for trace metals in edible fungi and the substrate role in accumulation of heavy metals. | levels of cadmium (cd), arsenic (as), mercury (hg), lead (pb), iron (fe), and zinc (zn) were investigated in 285 samples of 9 species of edible fungi (lentinus edodes, auricularia auricula, pleurotus ostreatus, tremella fuciformis, flammulina velutipes, agrocybe chaxinggu, armillaria mellea, agaricus bisporus, and pholiota nameko), which were collected from markets in beijing, china. in addition, edible fungi and culture substrates were collected from 7 cultivation bases to examine the role of t ... | 2015 | 26077507 |
| temperature-dependent development and reproductive traits of tyrophagus putrescentiae (sarcoptiformes: acaridae) reared on different edible mushrooms. | china is the largest producer, consumer, and exporter of mushrooms in the world. the storage mite, tyrophagus putrescentiae schrank, is one of the most important arthropod pests in mushroom cultivation. this study investigated the development and reproductive traits of this mite reared on four mushroom species: agaricus bisporus lange, pleurotus ostreatus kumm, auricularia polytricha (mont.) sacc., and flammulina velutipes (fr.) sing., at seven constant temperatures ranging from 16 to 34 °c at 8 ... | 2015 | 26313193 |
| a comprehensive review of tropical milky white mushroom (calocybe indica p&c). | a compressive description of tropical milky white mushroom (calocybe indica p&c var. apk2) is provided in this review. this mushroom variety was first identified in the eastern indian state of west bengal and can be cultivated on a wide variety of substrates, at a high temperature range (30~38℃). however, no commercial cultivation was made until 1998. krishnamoorthy 1997 rediscovered the fungus from tamil nadu, india and standardized the commercial production techniques for the first time in the ... | 2015 | 26539033 |
| regulation of primary metabolic pathways in oyster mushroom mycelia induced by blue light stimulation: accumulation of shikimic acid. | shikimic acid is a key intermediate in the aromatic amino acid pathway as well as an important starting material for the synthesis of tamiflu, a potent and selective inhibitor of the neuraminidase enzyme of influenza viruses a and b. here we report that in oyster mushroom (pleurotus ostreatus) mycelia cultivated in the dark, stimulation with blue light-emitting diodes induces the accumulation of shikimic acid. an integrated analysis of primary metabolites, gene expression and protein expression ... | 2015 | 25721093 |
| olive mill wastewater biodegradation potential of white-rot fungi--mode of action of fungal culture extracts and effects of ligninolytic enzymes. | forty-nine white-rot strains belonging to 38 species of basidiomycota were evaluated for olive-mill wastewater (omw) degradation. almost all fungi caused high total phenolics (>60%) and color (⩽ 70%) reduction, while cod and phytotoxicity decreased to a lesser extent. culture extracts from selected agrocybe cylindracea, inonotus andersonii, pleurotus ostreatus and trametes versicolor strains showed non-altered physicochemical and enzymatic activity profiles when applied to raw omw in the presenc ... | 2015 | 25879179 |
| can consumption of antioxidant rich mushrooms extend longevity?: antioxidant activity of pleurotus spp. and its effects on mexican fruit flies' (anastrepha ludens) longevity. | the variability of antioxidant capacity of 14 strains of the edible oyster mushroom pleurotus spp. was determined, and the effect of selected mushroom supplements on the longevity of the mexican fruit fly, anastrepha ludens, was evaluated. the antioxidant capacity of the fruiting bodies was determined by three different methods, measuring the free radical scavenging activity of methanolic extracts, the oh radical scavenging capacity, and the total phenol content. the inhibition percentage of the ... | 2015 | 26499817 |
| oyster culinary-medicinal mushroom, pleurotus ostreatus (higher basidiomycetes), growth in grain-based diet improves broiler chicken production. | many alternative compounds have been tested to improve poultry performance but few of them have previously used mycelial-colonized substrate to partially replace standard diet in broiler chickens. the objective of this study was to evaluate broiler chicken production, health, and meat sensory characteristics, with partial replacement of the standard diet by pleurotus ostreatus-colonized substrate. one hundred fifty 1-day-old male cobb chicks were given standard diet partially replaced by 0, 5, 1 ... | 2015 | 25746622 |
| hypoglycemic and antidiabetic effect of pleurotus sajor-caju aqueous extract in normal and streptozotocin-induced diabetic rats. | pleurotus sajor-caju (psc) is an edible oyster mushroom featuring high nutritional values and pharmacological properties. objective. to investigate the hypoglycemic and antidiabetic effects of single and repeated oral administration of psc aqueous extract in normal and diabetic rats. | 2015 | 26682215 |
| the effects of different substrates on the growth, yield, and nutritional composition of two oyster mushrooms (pleurotus ostreatus and pleurotus cystidiosus). | the study was conducted to compare the effects of different agro-wastes on the growth, yield, and nutritional composition of oyster mushrooms pleurotus ostreatus (po) and pleurotus cystidiosus (pc). seven substrate formulas including sawdust (sd), corncob (cc), sugarcane bagasse (sb) alone and in combination of 80 : 20, 50 : 50 ratio between sd and cc, sd and sb were investigated. the results indicated that different substrate formulas gave a significant difference in total colonization period, ... | 2015 | 26839502 |
| the effects of temperature and nutritional conditions on mycelium growth of two oyster mushrooms (pleurotus ostreatus and pleurotus cystidiosus). | the influences of temperature and nutritional conditions on the mycelium growth of oyster mushroom pleurotus ostreatus (po) and pleurotus cystidiosus (pc) were investigated in laboratory experiment during the summer season of 2014. the results of the experiment indicated that potato dextrose agar (pda) and yam dextrose agar (yda) were the most suitable media for the mycelium growth of oyster mushroom po while four media (pda, yda, sweet potato dextrose agar, and malt extract agar medium) were no ... | 2015 | 25892910 |
| hypoglycaemic activity of culinary pleurotus ostreatus and p. cystidiosus mushrooms in healthy volunteers and type 2 diabetic patients on diet control and the possible mechanisms of action. | this study determined the oral hypoglycaemic effect of suspensions of freeze dried and powdered (sfdp) pleurotus ostreatus (p.o) and pleurotus cystidiosus (p.c), using healthy human volunteers and type 2 diabetic patients on diet control at a dose of 50 mg/kg/body weight, followed by a glucose load. the possible hypoglycaemic mechanisms were evaluated using rats, by examining intestinal glucose absorption and serum levels of insulin, glucokinase (gk) and glycogen synthase kinase (gsk). the p.o a ... | 2015 | 25382404 |
| degradation of bunker c fuel oil by white-rot fungi in sawdust cultures suggests potential applications in bioremediation. | fungal lignocellulolytic enzymes are promising agents for oxidizing pollutants. this study investigated degradation of number 6 "bunker c" fuel oil compounds by the white-rot fungi irpex lacteus, trichaptum biforme, phlebia radiata, trametes versicolor, and pleurotus ostreatus (basidiomycota, agaricomycetes). averaging across all studied species, 98.1%, 48.6%, and 76.4% of the initial bunker c c10 alkane, c14 alkane, and phenanthrene, respectively were degraded after 180 days of fungal growth on ... | 2015 | 26111162 |
| biotransformation of fluoroquinolone antibiotics by ligninolytic fungi--metabolites, enzymes and residual antibacterial activity. | a group of white rot fungi (irpex lacteus, panus tigrinus, dichomitus squalens, trametes versicolor and pleurotus ostreatus) was investigated for the biodegradation of norfloxacin (nor), ofloxacin (of) and ciprofloxacin (cip). the selected fluoroquinolones were readily degraded almost completely by i. lacteus and t. versicolor within 10 and 14 d of incubation in liquid medium, respectively. the biodegradation products were identified by liquid chromatography-mass spectrometry. the analyses indic ... | 2015 | 25592459 |
| computational analysis and low-scale constitutive expression of laccases synthetic genes gllcc1 from ganoderma lucidum and poxa 1b from pleurotus ostreatus in pichia pastoris. | lacasses are multicopper oxidases that can catalyze aromatic and non-aromatic compounds concomitantly with reduction of molecular oxygen to water. fungal laccases have generated a growing interest due to their biotechnological potential applications, such as lignocellulosic material delignification, biopulping and biobleaching, wastewater treatment, and transformation of toxic organic pollutants. in this work we selected fungal genes encoding for laccase enzymes gllcc1 in ganoderma lucidum and p ... | 2015 | 25611746 |
| a high throughput colorimetric assay of β-1,3-d-glucans by congo red dye. | mushroom strains contain complex nutritional biomolecules with a wide spectrum of therapeutic and prophylactic properties. among these compounds, β-d-glucans play an important role in immuno-modulating and anti-tumor activities. the present work involves a novel colorimetric assay method for β-1,3-d-glucans with a triple helix tertiary structure by using congo red. the specific interaction that occurs between congo red and β-1,3-d-glucan was detected by bathochromic shift from 488 to 516 nm (>20 ... | 2015 | 25555819 |
| antioxidant capacity and total phenolics content of the fruiting bodies and submerged cultured mycelia of sixteen higher basidiomycetes mushrooms from india. | the fruiting bodies and the submerged cultured mycelia of 16 higher basidiomycetes mushrooms- agaricus bisporus, armillaria mellea, auricularia auricula-judae, ganoderma applanatum, g. lucidum, laetiporus sulphureus, lentinus tigrinus, lycoperdon pyriforme, phellinus linteus, pleurotus ostreatus, p. sajor-caju, polyporus arcularius, russula brevipes, schizophyllum commune, sparassis crispa, and spongipellis unicolor-from different taxonomic groups were examined for their antioxidant capacity (ao ... | 2015 | 26756185 |
| effects of pleurotus eryngii polysaccharides on bacterial growth, texture properties, proteolytic capacity, and angiotensin-i-converting enzyme-inhibitory activities of fermented milk. | pleurotus eryngii is one of the most favored oyster mushrooms and contains various beneficial bioactive compounds. polysaccharide extracted from p. eryngii (peps) was added as a natural-source ingredient to milk before fermentation, and the effects of additional peps on fermented milk were investigated in this study. the peps were extracted and added to reconstituted skim milk (12%, wt/vol) at 0.5, 0.25, and 0.125% (wt/vol) and fermented by a non-exopolysaccharide-producing strain, streptococcus ... | 2015 | 25747830 |
| hydrodynamics, fungal physiology, and morphology. | filamentous cultures, such as fungi and actinomycetes, contribute substantially to the pharmaceutical industry and to enzyme production, with an annual market of about 6 billion dollars. in mechanically stirred reactors, most frequently used in fermentation industry, microbial growth and metabolite productivity depend on complex interactions between hydrodynamics, oxygen transfer, and mycelial morphology. the dissipation of energy through mechanically stirring devices, either flasks or tanks, im ... | 2015 | 25652005 |
| mushroom extracts induce human colon cancer cell (colo-205) death by triggering the mitochondrial apoptosis pathway and go/g1-phase cell cycle arrest. | functional foods are extensively studied for their cancer preventive effects. in the present study, we compared the anti-cancer activity of aqueous extracts of three species of mushrooms including: pleurotus ostreatus (pae), auricularia polytricha (aae) and macrolepiota procera (mae) on colo-205 cells. | 2015 | 25959910 |
| production of cellobionate from cellulose using an engineered neurospora crassa strain with laccase and redox mediator addition. | we report a novel production process for cellobionic acid from cellulose using an engineered fungal strain with the exogenous addition of laccase and a redox mediator. a previously engineered strain of neurospora crassa (f5∆ace-1∆cre-1∆ndvb) was shown to produce cellobionate directly from cellulose without the addition of exogenous cellulases. specifically, n. crassa produces cellulases, which hydrolyze cellulose to cellobiose, and cellobiose dehydrogenase (cdh), which oxidizes cellobiose to cel ... | 2015 | 25849253 |
| screening of antimicrobial, antioxidant properties and bioactive compounds of some edible mushrooms cultivated in bangladesh. | for a long time mushrooms have been playing an important role in several aspects of the human activity. recently edible mushrooms are used extensively in cooking and make part of new food in bangladesh for their beneficial properties. the aim of this study is to screen some values of mushrooms used in bangladesh. | 2015 | 25858107 |
| bioactivity of volatile organic compounds produced by pseudomonas tolaasii. | pseudomonas tolaasii is the main bacterial pathogen of several mushroom species. in this paper we report that strains of p. tolaasii produce volatile substances inducing in vitro mycelia growth inhibition of pleurotus ostreatus and p. eryngii, and agaricus bisporus and p. ostreatus basidiome tissue blocks brown discoloration. p. tolaasii strains produced the volatile ammonia but not hydrogen cyanide. among the volatiles detected by gc-ms, methanethiol, dimethyl disulfide (dmds), and 1-undecene w ... | 2015 | 26500627 |
| efficacy of fungal decolorization of a mixture of dyes belonging to different classes. | dyes are the most difficult constituents to remove by conventional biological wastewater treatment. colored wastewater is mainly eliminated by physical and chemical procedures, which are very expensive and have drawbacks. therefore, the advantage of using biological processes, such as the biotransformation of dyes, is that they may lead to complete mineralization or formation of less toxic products. to prove the possibility of using fungal processes for decolorization and other applications, the ... | 2015 | 26273256 |
| effect of solid state fermentation medium optimization on pleurotus ostreatus laccase production. | the objective of this work was to increase laccase production by pleurotus ostreatus plab through culture medium optimization using solid state culture conditions. increased laccase activity was obtained through design of experiments (doe) using the taguchi orthogonal array (oa). seven factors, viz. lignocellulose, glucose, yeast extract, peptone, kh(2)po(4), mgso(4) 7h(2)o and mnso(4) h(2)o at three levels and ph at two levels. oa layout of l18 (2(1) x 3(7)) was selected for the proposed experi ... | 2015 | 26680722 |
| submerged cultivation of mycelium with high ergothioneine content from the culinary-medicinal golden oyster mushroom, pleurotus citrinopileatus (higher basidiomycetes). | the optimization of submerged culture of the culinary-medicinal golden oyster mushroom, pleurotus citrinopileatus, was studied using a one-factor-at-a-time, two-stage stimulation and central composite rotatable design to produce mycelia with high ergothioneine content. the optimal culture conditions for mycelia harvested at day 22 were a temperature of 25°c, an inoculation ratio of 5%, 2% glucose, 0.5% yeast extract, and adjustment of the initial ph value to 10. the biomass and ergothioneine con ... | 2015 | 26559861 |
| hydrophilic compounds in liquids of enzymatic hydrolyzed spruce and pine biomass. | organic acids are used for starting compounds in material sciences and in biorefinery, food, fuel, pharmaceutical, and medical industry. here, we provide the data from a biochemical approach made to investigate production of organic acids and isolation of metals from wood, which is the most abundant biomass. spruce and bark, phloem, and heartwood from pine were fermented with either microbes of oyster mushroom (pleurotus ostreatus), baker's yeast, or lactic acid bacteria to improve selective fer ... | 2015 | 26543881 |
| differential expression of laccase genes in pleurotus ostreatus and biochemical characterization of laccase isozymes produced in pichia pastoris. | in this study, transcriptome analysis of twelve laccase genes in pleurotus ostreatus revealed that their expression was differentially regulated at different developmental stages. lacc5 and lacc12 were specifically expressed in fruiting bodies and primordia, respectively, whereas lacc6 was expressed at all developmental stages. lacc1 and lacc3 were specific to the mycelial stage in solid medium. in order to investigate their biochemical characteristics, these laccases were heterologously express ... | 2015 | 26539044 |
| iron bioaccumulation in mycelium of pleurotus ostreatus. | pleurotus ostreatus is able to bioaccumulate several metals in its cell structures; however, there are no reports on its capacity to bioaccumulate iron. the objective of this study was to evaluate cultivation variables to increase iron bioaccumulation in p. ostreatus mycelium. a full factorial design and a central composite design were utilized to evaluate the effect of the following variables: nitrogen and carbon sources, ph and iron concentration in the solid culture medium to produce iron bio ... | 2015 | 26221108 |
| statistical optimization of laccase production and delignification of sugarcane bagasse by pleurotus ostreatus in solid-state fermentation. | laccases are oxidative enzymes related to the degradation of phenolic compounds, including lignin units, with concomitant reduction of oxygen to water. delignification is a necessary pretreatment step in the process of converting plant biomass into fermentable sugars. the objective of this work was to optimize the production of laccases and to evaluate the delignification of sugarcane bagasse by pleurotus ostreatus in solid-state fermentation. among eight variables (ph, water activity, temperatu ... | 2015 | 26180784 |
| hydrophilic compounds in liquids of enzymatic hydrolyzed spruce and pine biomass. | this study was focused on organic acids and metals in biofluids of wood. without seasoning, fresh woods from spruce and bark, phloem, and heartwood from pine were used as materials, which were degraded with either microbes of oyster mushroom, baker's yeast, or lactic acid bacteria. due to neutral ph of the fluids, ambient temperature, atmospheric pressure, and short reaction time, native wood microbe populations were supposed to be present. the water content of the fresh woods was 4 to 20%. the ... | 2015 | 26070851 |
| detoxification of 5-hydroxymethylfurfural by the pleurotus ostreatus lignolytic enzymes aryl alcohol oxidase and dehydrogenase. | current large-scale pretreatment processes for lignocellulosic biomass are generally accompanied by the formation of toxic degradation products, such as 5-hydroxymethylfurfural (hmf), which inhibit cellulolytic enzymes and fermentation by ethanol-producing yeast. overcoming these toxic effects is a key technical barrier in the biochemical conversion of plant biomass to biofuels. pleurotus ostreatus, a white-rot fungus, can efficiently degrade lignocellulose. in this study, we analyzed the abilit ... | 2015 | 25897324 |
| benzoic acid derivatives with improved antifungal activity: design, synthesis, structure-activity relationship (sar) and cyp53 docking studies. | previously, we identified cyp53 as a fungal-specific target of natural phenolic antifungal compounds and discovered several inhibitors with antifungal properties. in this study, we performed similarity-based virtual screening and synthesis to obtain benzoic acid-derived compounds and assessed their antifungal activity against cochliobolus lunatus, aspergillus niger and pleurotus ostreatus. in addition, we generated structural models of cyp53 enzyme and used them in docking trials with 40 selecte ... | 2015 | 26154240 |
| antimicrobial activity of extracts of the oyster culinary medicinal mushroom pleurotus ostreatus (higher basidiomycetes) and identification of a new antimicrobial compound. | pleurotus ostreatus is an edible mushroom that also has high medicinal values. in this study, p. ostreatus was tested for its ability to inhibit the growth of fungi and bacteria. the freeze-dried fruiting body, broth from submerged culture, and mycelial biomass of p. ostreatus were extracted using alcohols and water as solvents. the extracts were then tested for their antimicrobial activity against the growth of fungi and bacteria. it was observed that the water extract from fruiting bodies had ... | 2015 | 26349515 |
| aflatoxin b1 degradation during co-cultivation of aspergillus flavus and pleurotus ostreatus strains on rice straw. | aflatoxin b1 (afb1) produced by aspergillus flavus is known to have carcinogenic and teratogenic effects on animal health. accidental feeding of afb1-contaminated rice straw may be detrimental to dairy cattle. white-rot basidiomycetous fungus pleurotus ostreatus can grow on different agronomic wastes by synthesizing different ligninolytic enzymes. these extracellular enzymes are capable of degrading many environmentally hazardous compounds including afb1. the present study examines the ability o ... | 2015 | 28324293 |
| antioxidant and antifungal potential of pleurotus ostreatus and agrocybe cylindracea basidiocarps and mycelia. | basidiocarps of pleurotus ostreatus and agrocybe cylindracea are characterized by high nutritional value and numerous medicinal activities, though bioactivities of their mycelia have not been sufficiently studied. the aim of the study was to evaluate antioxidant and antifungal potentials of p. ostreatus and a. cylindracea basidiocarp extracts, as well as those obtained from mycelia cultivated in common synthetic medium and media enriched with various agro-industrial residues. the free radical sc ... | 2015 | 25483715 |
| characterization of the highly active fragment of glyceraldehyde-3-phosphate dehydrogenase gene promoter for recombinant protein expression in pleurotus ostreatus. | developing efficient native promoters is important for improving recombinant protein expression by fungal genetic engineering. the promoter region of glyceraldehyde-3-phosphate dehydrogenase gene in pleurotus ostreatus (pogpd) was isolated and optimized by upstream truncation. the activities of these promoters with different lengths were further confirmed by fluorescence, quantitative real-time pcr and western blot analysis. a truncated pogpd-p2 fragment (795 bp) drove enhanced green fluorescenc ... | 2015 | 25743073 |
| effect of the replacement of aspartic acid/glutamic acid residues with asparagine/glutamine residues in rnase he1 from hericium erinaceus on inhibition of human leukemia cell line proliferation. | rnase he1 from hericium erinaceus, a member of the rnase t1 family, has high identity with rnase po1 from pleurotus ostreatus with complete conservation of the catalytic sequence. however, the optimal ph for rnase he1 activity is lower than that of rnase po1, and the enzyme shows little inhibition of human tumor cell proliferation. hence, to investigate the potential antitumor activity of recombinant rnase he1 and to possibly enhance its optimum ph, we generated rnase he1 mutants by replacing 12 ... | 2015 | 25338779 |
| absence of a gene encoding cytosine deaminase in the genome of the agaricomycete coprinopsis cinerea enables simple marker recycling through 5-fluorocytosine counterselection. | coprinopsis cinerea is a model species for molecular genetics studies of sexual development in agaricomycetes or homobasidiomycetes. recently, efficient gene targeting was established in this fungus by generating cc.ku70 or cc.lig4 disruptants. to determine the molecular mechanisms underlying sexual development, which involves many genes, generating multiple gene disruptants is required. however, the number of transformation markers available for c. cinerea is limited. this problem would be solv ... | 2015 | 26223587 |
| the amoebicidal effect of ergosterol peroxide isolated from pleurotus ostreatus. | dysentery is an inflammation of the intestine caused by the protozoan parasite entamoeba histolytica and is a recurrent health problem affecting millions of people worldwide. because of the magnitude of this disease, finding novel strategies for treatment that does not affect human cells is necessary. ergosterol peroxide is a sterol particularly known as a major cytotoxic agent with a wide spectrum of biological activities produced by edible and medicinal mushrooms. the aim of this report is to ... | 2015 | 26392373 |
| description of the first fungal dye-decolorizing peroxidase oxidizing manganese(ii). | two phylogenetically divergent genes of the new family of dye-decolorizing peroxidases (dyps) were found during comparison of the four dyp genes identified in the pleurotus ostreatus genome with over 200 dyp genes from other basidiomycete genomes. the heterologously expressed enzymes (pleos-dyp1 and pleos-dyp4, following the genome nomenclature) efficiently oxidize anthraquinoid dyes (such as reactive blue 19), which are characteristic dyp substrates, as well as low redox-potential dyes (such as ... | 2015 | 25967658 |
| nutritional characterisation of pleurotus ostreatus (jacq. ex fr.) p. kumm. produced using paper scraps as substrate. | pleurotus ostreatus (jacq. ex fr.) p. kumm. is the third most produced edible mushroom worldwide, due to its ability to colonise and degrade a large variety of lignocellulosic substrates. therefore, the objective of this work was to evaluate the chemical composition of fruiting bodies of p. ostreatus grown on blank and printed paper substrates, in comparison with samples grown on oat straw (control). the nutritional properties of the control sample were similar to values reported in the literatu ... | 2015 | 25236243 |
| a novel stirrer design and its application in submerged fermentation of the edible fungus pleurotus ostreatus. | in this study, a straight diagonal-pitched blade stirrer was designed, built and characterized in a 5-l fermenter. compared with the six straight blade rushton turbine, the power consumption of the new stirrer is lower at a given speed under conditions of no ventilation. the oxygen transference is poorer at the same agitation speed in the cultivation conditions and scales investigated, which confirms that the shear stress of the new stirrer is lower and the gas dispersion is weaker. the new stir ... | 2015 | 25234512 |
| genetic diversity of kenyan native oyster mushroom (pleurotus). | members of the genus pleurotus, also commonly known as oyster mushroom, are well known for their socioeconomic and biotechnological potentials. despite being one of the most important edible fungi, the scarce information about the genetic diversity of the species in natural populations has limited their sustainable utilization. a total of 71 isolates of pleurotus species were collected from three natural populations: 25 isolates were obtained from kakamega forest, 34 isolates from arabuko sokoke ... | 2015 | 25344263 |
| modelling the influence of time and temperature on the respiration rate of fresh oyster mushrooms. | the respiration rate of mushrooms is an important indicator of postharvest senescence. storage temperature plays a major role in their rate of respiration and, therefore, in their postharvest life. in this context, reliable predictions of respiration rates are critical for the development of modified atmosphere packaging that ultimately will maximise the quality of the product to be presented to consumers. this work was undertaken to study the influence of storage time and temperature on the res ... | 2015 | 25339381 |
| nanoparticle and polysaccharide conjugate: a potential candidate vaccine to improve immunological stimuli. | active polysaccharides isolated from various fungal sources have been implicated to stimulate immune response against various pathogens as well as self anomalies such as cancer. therefore, the nuanced approach presented in our work was to blend polysaccharides derived from pleurotus ostreatus with biocompatible ferrite nanoparticles and thereafter investigate the enhanced immune functionality of the polysaccharide-nanoparticle composite. a schiff base reductive amination reaction occurred betwee ... | 2015 | 25450832 |
| haematopoiesis radioprotection in balb/c mice by an aqueous mycelium extract from the basidiomycete pleurotus ostreatus mushroom. | the study examined the radioprotective activity of an aqueous extract from pleurotus ostreatus mycelium administered to balb/c mice. male mice were whole-body irradiated on day 0 ((60)co, at 0.43 gy/min) and divided into two groups. the extract was administered intraperitoneally to one group (100 mg/kg) on days - 10 to - 6 and - 2 to +1 with respect to the irradiation. the irradiated-control group was injected with saline solution; non-irradiated mice were used as negative controls. the radiopro ... | 2015 | 25423080 |
| effects of calmodulin on expression of lignin-modifying enzymes in pleurotus ostreatus. | previously, we suppressed the expression of genes encoding isozymes of lignin peroxidase (lip) and manganese peroxidase (mnp) using a calmodulin (cam) inhibitor, w7, in the white-rot fungus phanerochaete chrysosporium; this suggested that cam positively regulates their expression. here, we studied the role of cam in another white-rot fungus, pleurotus ostreatus, which produces mnp and versatile peroxidase (vp), but not lip. w7 upregulated mn(2+)-dependent oxidation of guaiacol, suggesting that c ... | 2015 | 25407463 |
| laccase production by pleurotus ostreatus and its application in synthesis of gold nanoparticles. | in this work, the production of fungal laccase was optimized from local isolate of pleurotus ostreatus using solid state fermentation. factorial design was used to study the effect of several nutrients on enzyme production. purification and characterization of the enzyme and the effect of temperature, ph and gamma radiation on fungal growth and enzyme production was investigated. optimization of production conditions yielded an enzyme with activity over 32,450 iu/g of fermented substrate. factor ... | 2015 | 28626680 |
| immune augmentation and dalton's lymphoma tumor inhibition by glucans/glycans isolated from the mycelia and fruit body of pleurotus ostreatus. | with the increase in cancer progression, alternatives in the medicinal field with minimal side effects need to be ascertained. in this context, for the first time novel glucans/glycans isolated from the mycelia and fruit body of pleurotus ostreatus have been compared for their exquisite property as immunoceuticals. glucans from both the sources displayed immunological functions which include lymphocyte proliferation, macrophage activation (nitric oxide production, ros generation, phagocytosis, t ... | 2015 | 25662752 |
| identification of angiotensin-converting enzyme inhibitory proteins from mycelium of pleurotus pulmonarius (oyster mushroom). | pleurotus pulmonarius (grey oyster mushroom) has been acknowledged as a recuperative agent for many diseases in addition to its recognition as a nutritious provision. we performed a study on p. pulmonarius mycelium for an antihypertensive effect via the angiotensin-converting enzyme inhibitory activity. the preliminary assay on the mycelial water extract demonstrated that the angiotensin-converting enzyme inhibitory activity had an ic50 value of 720 µg/ml. further protein purifications via ammon ... | 2015 | 25590365 |
| combination of biochar amendment and mycoremediation for polycyclic aromatic hydrocarbons immobilization and biodegradation in creosote-contaminated soil. | soils impregnated with creosote contain high concentrations of polycyclic aromatic hydrocarbons (pah). to bioremediate these soils and avoid pah spread, different bioremediation strategies were tested, based on natural attenuation, biochar application, wheat straw biostimulation, pleurotus ostreatus mycoremediation, and the novel sequential application of biochar for 21 days and p. ostreatus 21 days more. soil was sampled after 21 and 42 days after the remediation application. the efficiency and ... | 2015 | 25506817 |
| the ligninolytic peroxidases in the genus pleurotus: divergence in activities, expression, and potential applications. | mushrooms of the genus pleurotus are comprised of cultivated edible ligninolytic fungi with medicinal properties and a wide array of biotechnological and environmental applications. like other white-rot fungi (wrf), they are able to grow on a variety of lignocellulosic biomass substrates and degrade both natural and anthropogenic aromatic compounds. this is due to the presence of the non-specific oxidative enzymatic systems, which are mainly consisted of lacasses, versatile peroxidases (vps), an ... | 2015 | 25503316 |
| enhancing stress tolerance by overexpression of a methionine sulfoxide reductase a (msra) gene in pleurotus ostreatus. | proteins are subjected to modification by reactive oxygen species (ros), and oxidation of specific amino acid residues can impair their biological functions. methionine as a sulfur-containing amino acid is easily oxidized to methionine sulfoxide (metso). the modified methionine can be repaired by methionine sulfoxide reductase (msr), an enzyme that reverses oxidation of methionine in proteins. in this study, a methionine sulfoxide reductase a (pomsra) gene from pleurotus ostreatus was cloned and ... | 2015 | 25573474 |
| erratum to: effects of calmodulin on expression of lignin-modifying enzymes in pleurotus ostreatus. | 2015 | 25572694 | |
| effect of the ostreolysin a/pleurotolysin b pore-forming complex on neuroblastoma cell morphology and intracellular ca²⁺ activity. | ostreolysin a (olya) and pleurotolysin b (plyb), isolated from edible oyster mushrooms, form a cytolytic complex (olya/plyb) in membrane cells that causes respiratory arrest. this study evaluated the mechanisms underlying cytotoxic olya/plyb activity in neuroblastoma ng108-15 cells. confocal microscopy with morphometric analysis revealed that olya/plyb increased the 3-dimensional projected area of differentiated cells. iso-osmotic replacement of nacl by sucrose or na-isethionate prevented the ce ... | 2015 | 25556216 |
| structure characterization and antitumor activity of a polysaccharide from the alkaline extract of king oyster mushroom. | a water-soluble polysaccharide, designated as komap, was isolated and purified from the alkaline extract of king oyster mushroom, which was composed of glucose (glc), mannose (man) and arabinose (ara) in a molar ratio of 6.2:2.1:2.0. it had an average molecular weight of 2.1×10(4)da. gc-ms analysis revealed that komap was a linear structure of the polymer with a backbone composed of β-1,4-linked glucopyranosyl and β-1,3,6-linked mannopyranosyl units, which was terminated with α-1-linked arabinof ... | 2015 | 25542113 |
| antitumor activity of polysaccharide extracted from pleurotus ostreatus mycelia against gastric cancer in vitro and in vivo. | the present study aimed to determine the antitumor effects of polysaccharides extracted from pleurotus ostreatus mycelium on gastric cancer in vitro and in vivo. polysaccharides were extracted from pleurotus ostreatus mycelium and an antitumor component, known as pleurotus ostreatus mycelium polysaccharides 2 (pomp2), with a relative molecular weight of 29 kda, was then sequentially purified using sephadex g200 size-exclusion chromatography and diethylaminoethyl-52 cellulose ion-exchange chromat ... | 2015 | 25892617 |
| analysis of environmental stress factors using an artificial growth system and plant fitness optimization. | the environment promotes evolution. evolutionary processes represent environmental adaptations over long time scales; evolution of crop genomes is not inducible within the relatively short time span of a human generation. extreme environmental conditions can accelerate evolution, but such conditions are often stress inducing and disruptive. artificial growth systems can be used to induce and select genomic variation by changing external environmental conditions, thus, accelerating evolution. by ... | 2015 | 25874206 |
| validation of reference genes for transcriptional analyses in pleurotus ostreatus by using reverse transcription-quantitative pcr. | recently, the lignin-degrading basidiomycete pleurotus ostreatus has become a widely used model organism for fungal genomic and transcriptomic analyses. the increasing interest in this species has led to an increasing number of studies analyzing the transcriptional regulation of multigene families that encode extracellular enzymes. reverse transcription (rt) followed by real-time pcr is the most suitable technique for analyzing the expression of gene sets under multiple culture conditions. in th ... | 2015 | 25862220 |
| immunomodulatory and immunorestorative activities of β-d-glucan-rich extract and polysaccharide fraction of mushroom, pleurutus tuberregium. | some edible mushrooms are reputed to possess useful medicinal properties which are related to their ability to modulate the protective responses of the immune system. | 2015 | 25857364 |
| thin-layer modeling of convective and microwave-convective drying of oyster mushroom (pleurotus ostreatus). | oyster mushroom samples were dried under selected convective, microwave-convective drying conditions in a recirculatory hot-air dryer and microwave assisted hot-air dryer (2.45 ghz, 1.5 kw) respectively. only falling rate period and no constant rate period, was exhibited in both the drying technique. the experimental moisture loss data were fitted to selected semi-theoretical thin-layer drying equations. the mathematical models were compared according to three statistical parameters, i.e. correl ... | 2015 | 25829581 |
| ultrafast excited-state charge-transfer dynamics in laccase type i copper site. | femtosecond pump-probe spectroscopy was used to investigate the excited state dynamics of the t1 copper site of laccase from pleurotus ostreatus, by exciting its 600 nm charge transfer band with a 15-fs pulse and probing over a broad range in the visible region. the decay of the pump-induced ground-state bleaching occurs in a single step and is modulated by clearly visible oscillations. global analysis of the two-dimensional differential transmission map shows that the excited state exponentiall ... | 2015 | 25819432 |
| biosorption of (241)am from aqueous solutions and its biochemical fractionation in pleurotus ostreatus mycelium. | 2015 | 25772987 | |
| determination of lovastatin, β-glucan, total polyphenols, and antioxidant activity in raw and processed oyster culinary-medicinal mushroom, pleurotus ostreatus (higher basidiomycetes). | the objective of this study was to quantify a number of bioactive compounds and antioxidant activity of the oyster mushroom, pleurotus. ostreatus, and characterize the effects of processing, such as blanching, on these outcomes. dry matter content was 8%. lovastatin was not detected in this study. β-glucan content of 23.9% and total polyphenol content of 487.12 mg gallic acid equivalent/100 g of dry matter were obtained in raw p. ostreatus. antioxidant activities as evaluated by 1,1-diphenyl-2-p ... | 2015 | 25746617 |
| [molecular cloning and characterization of two oxidative stress related genes from medicinal fungus polyporus umbellatus]. | to clone the nadph gene (punox) and glyoxal oxidase gene (puglox) from a medicinal fungus polyporus umbellatus, and to carry out the bioinformatic analysis. | 2015 | 26939456 |
| a study on 17alpha-ethinylestradiol metabolism in rat and pleurotus ostreatus. | 17α-ethinylestradiol (ee2) is an endocrine disruptor that is an ingredient of oral contraceptives. here, ee2 metabolism catalyzed by cytochromes p450 (cyp) was studied. two model organisms, rat and ligninolytic fungus pleurotus ostreatus, were used. | 2015 | 26757108 |
| identification of the nucleophile catalytic residue of gh51 α-l-arabinofuranosidase from pleurotus ostreatus. | in this study, the recombinant α-l-arabinofuranosidase from the fungus pleurotus ostreatus (rpoabf) was subjected to site-directed mutagenesis in order to identify the catalytic nucleophile residue. based on bioinformatics and homology modelling analyses, e449 was revealed to be the potential nucleophilic residue. thus, the mutant e449g of poabf was recombinantly expressed in pichia pastoris and its recombinant expression level and reactivity were investigated in comparison to the wild-type. the ... | 2015 | 26690659 |
| optimization of arundo donax saccharification by (hemi)cellulolytic enzymes from pleurotus ostreatus. | an enzymatic mixture of cellulases and xylanases was produced by pleurotus ostreatus using microcrystalline cellulose as inducer, partially characterized and tested in the statistical analysis of arundo donax bioconversion. the plackett-burman screening design was applied to identify the most significant parameters for the enzymatic hydrolysis of pretreated a. donax. as the most significant influence during the enzymatic hydrolysis of a. donax was exercised by the temperature (°c), ph, and time, ... | 2015 | 26634214 |
| characterisation of the large-scale production process of oyster mushroom (pleurotus ostreatus) with the analysis of succession and spatial heterogeneity of lignocellulolytic enzyme activities. | oyster mushroom (pleurotus ostreatus) lignocellulolytic enzyme activity pattern and variation was investigated in a large-scale facility from spawning until the end of the second flush. in the first cultivation cycle laccase production reached its peak during vegetative growth stage, while manganese-peroxidase showed the highest activity during fruiting body induction. cellulose and hemicellulose degrading enzymes had maximal activity at the beginning of flush and harvest stage. the enzyme activ ... | 2015 | 26615756 |
| long term storage of pleurotus ostreatus and trametes versicolor isolates using different cryopreservation techniques and its impact on laccase activity. | the strain pleurotus ostreatus florida f6, its 45 basidiospore-derived isolates (both monokaryons and dikaryons prepared in our laboratory), trametes versicolor strain ccbas 614 and 22 other t. versicolor isolates obtained from the sporocarps collected in distant localities were successfully preserved for 12 y using perlite and straw cryopreservation protocols. all tested isolates survived a 12-year storage in liquid nitrogen (ln) and their laccase production and poly b411 decolorization capacit ... | 2015 | 26615755 |
| the contribution of phenolic acids to the anti-inflammatory activity of mushrooms: screening in phenolic extracts, individual parent molecules and synthesized glucuronated and methylated derivatives. | in the present study, the ethanolic extracts of fourteen edible mushrooms were investigated for their anti-inflammatory potential in lps (lipopolysaccharide) activated raw 264.7 macrophages. furthermore the extracts were chemically characterized in terms of phenolic acids and related compounds. the identified molecules (p-hydroxybenzoic, p-coumaric and cinnamic acids) and their glucuronated and methylated derivatives obtained by chemical synthesis were also evaluated for the same bioactivity, in ... | 2015 | 28455068 |
| transformation pathways of the recalcitrant pharmaceutical compound carbamazepine by the white-rot fungus pleurotus ostreatus: effects of growth conditions. | the widely used anticonvulsant pharmaceutical carbamazepine is recalcitrant in many environmental niches and thus poses a challenge in wastewater treatment. we followed the decomposition of carbamazepine by the white-rot fungus pleurotus ostreatus in liquid culture compared to solid-state fermentation on lignocellulosic substrate where different enzymatic systems are active. carbamazepine metabolites were identified using liquid chromatography-high-resolution mass spectrometry (lc-q-tof-ms). in ... | 2015 | 26418858 |
| the amphiphilic hydrophobin vmh2 plays a key role in one step synthesis of hybrid protein-gold nanoparticles. | we report a simple and original method to synthesize gold nanoparticles in which a fungal protein, the hydrophobin vmh2 from pleurotus ostreatus and dicarboxylic acid-terminated polyethylene-glycol (peg) has been used as additional components in a one step process, leading to hybrid protein-metal nanoparticles (nps). the nanoparticles have been characterized by ultra-violet/visible, infrared and x-ray photoelectron spectroscopies, dynamic light scattering and also by electron microscopy imaging. ... | 2015 | 26402419 |
| electrochemistry combined with lc-hrms: elucidating transformation products of the recalcitrant pharmaceutical compound carbamazepine generated by the white-rot fungus pleurotus ostreatus. | transformation products (tps) of environmental pollutants must be identified to understand biodegradation processes and reaction mechanisms and to assess the efficiency of treatment processes. the combination of oxidation by an electrochemical cell (ec) with analysis by liquid chromatography-high-resolution mass spectrometry (lc-hrms) is a rapid approach for the determination and identification of tps generated by natural microbial processes. electrochemically generated tps of the recalcitrant p ... | 2015 | 26348877 |
| effect of packaging materials on the chemical composition and microbiological quality of edible mushroom (pleurotus ostreatus) grown on cassava peels. | edible fungi such as mushrooms are highly perishable and deteriorate few days after harvest due to its high moisture content and inability to maintain their physiological status. in this study, the effect of packaging materials on the nutritional composition of mushroom cultivated from cassava peels was investigated. mushroom samples were dried at 50°c in a cabinet dryer for 8 h. the dried mushroom samples packaged in four different packaging materials; high density polyethylene (hdpe), polyprop ... | 2015 | 26288720 |