| production, purification and characterisation of polysaccharides from pleurotus ostreatus with antitumour activity. | mushroom polysaccharides play an important role in functional foods because they exhibit biological modulator properties such as antitumour, antiviral and antibacterial activities. the present study involved the production, purification and characterisation of intracellular and extracellular free and protein-bound polysaccharides from pleurotus ostreatus and the investigation of their growth-inhibitory effect on human carcinoma cell lines. | 2012 | 22234986 |
| [effect of polycyclic aromatic hydrocarbons on laccase production by white rot fungus pleurotus ostreatus d1]. | the effect of polycyclic aromatic hydrocarbons (pahs) on the dynamics of laccase production by the fungus pleurotus ostreatus d1 under conditions of submerged cultivation on kirk's medium has been studied. it has been shown that phenanthrene, fluoranthene, pyrene, and chrysene actively induce this enzyme, whereas fluorene and anthrecene had a smaller effect. addition of mn2+ ions to cultivation medium elevates the laccase activity twofold and more in the presence of all the studied pahs. electro ... | 2016 | 22232903 |
| determination of optimal extraction time and temperature by response surface methodology to obtain high-level antioxidant activity in culinary-medicinal oyster mushroom, pleurotus ostreatus (jacq.:fr.) p. kumm. (higher basidiomycetes). | a central composite design of response surface methodology (rsm) was employed to optimize the extraction time (x1: 266.4-393.6 min) and temperature (x2: 42.9-57.1°c) of pleurotus ostreatus aqueous extract with high antioxidant activities, namely dpph radical-scavenging activity, abts radical cation inhibition, and ferric reducing/antioxidant power, as well as total phenolic content (tpc). results showed that the data were adequately fitted into four second-order polynomial models developed by rs ... | 2012 | 23510253 |
| activity of crude cold-water extract of the culinary-medicinal oyster mushroom, pleurotus ostreatus (jacq.:fr.) p.kumm. (higher basidiomycetes), and timolol maleate on induced ocular hypertension. | aqueous crude cold-water extract from the fruiting body of the culinary-medicinal oyster mushroom pleurotus ostreatus was assessed for activity against increased intra-ocular pressure (iop) in mice. a 0.1% dexamethasone instillation was used to raise the intra-ocular pressure in the animals above the 21-mmhg threshold limit. the extract has intrinsic anti-hypertensive properties that are dose dependent. a comparison analysis indicated that 150 mg/ml of the crude extract produced 57.69% reduction ... | 2012 | 23510215 |
| oral hypoglycemic activity of culinary-medicinal mushrooms pleurotus ostreatus and p. cystidiosus (higher basidiomycetes) in normal and alloxan-induced diabetic wistar rats. | this study investigates the oral hypoglycemic activity of pleurotus ostreatus (p.o.) and p. cystidiosus (p.c.) mushrooms on normal and alloxan-induced diabetic wistar rats. different doses (250, 500, 750, 1000, and 1250 mg/kg/body weight) of suspensions of freeze-dried and powdered (sfdp) p.o. and p.c. were administered to normal rats, and postprandial serum glucose levels were measured. optimal time of activity was investigated using the dose 500 mg/kg. hypoglycemic effect of a single dose of s ... | 2012 | 23510172 |
| self-assembly of hydrophobin protein rodlets studied with atomic force spectroscopy in dynamic mode. | we have investigated the self-assembling properties of the class i hydrophobin vmh2 isolated from the fungus pleurotus ostreatus. five different hydrophobin self assembled samples including monolayers, bilayers, and rodlets have been prepared by langmuir technique and studied at the nanoscale. local wettability and visco-elasticity of the different hydrophobins samples were obtained from atomic force spectroscopy experiments in dynamic mode performed at different, controlled relative humidity (r ... | 2012 | 22181848 |
| strategies for dephenolization of raw olive mill wastewater by means of pleurotus ostreatus. | the reduction of polyphenols content in olive mill wastewater (omw) is a major issue in olive oil manufacturing. although researchers have pointed out the potential of white-rot fungus in dephenolizing omw, the results available in the literature mainly concern pretreated (sterilized) omw. this paper deals with the reduction of polyphenols content in untreated omw by means of a white-rot fungus, pleurotus ostreatus. dephenolization was performed both in an airlift bioreactor and in aerated flask ... | 2012 | 22179541 |
| pleurotus ostreatus spent mushroom compost as green biosorbent for nickel (ii) biosorption. | the potential of pleurotus ostreatus spent mushroom compost (psmc) as a green biosorbent for nickel (ii) biosorption was investigated in this study. a novel approach of using the half-saturation concentration of biosorbent to rapidly determine the uptake, kinetics and mechanism of biosorption was employed together with cost per unit uptake analysis to determine the potential of this biosorbent. fifty per cent nickel (ii) biosorption was obtained at a half-saturation constant of 0.7 g biosorbent ... | 2011 | 22170837 |
| cultivation of the culinary-medicinal lung oyster mushroom, pleurotus pulmonarius (fr.) quél. (agaricomycetideae) on grass plants in taiwan. | cultivation of the culinary-medicinal lung oyster mushroom, pleurotus pulmonarius, on the stalks of three grass plants, i.e., panicum repens, pennisetum purpureum, and zea mays were investigated. the effects of various combinatorial substrates on mushroom mycelial growth and yield calculated as biological efficiency (be) were determined. among 9 experimental substrates, the most suitable substrate for mycelial growth was 45zms:45s, followed by 45prs:45s; their mycelial growth rates were obviousl ... | 2011 | 22135896 |
| pleurotus ostreatus heme peroxidases: an in silico analysis from the genome sequence to the enzyme molecular structure. | an exhaustive screening of the pleurotus ostreatus genome was performed to search for nucleotide sequences of heme peroxidases in this white-rot fungus, which could be useful for different biotechnological applications. after sequence identification and manual curation of the corresponding genes and cdnas, the deduced amino acid sequences were converted into structural homology models. a comparative study of these sequences and their structural models with those of known fungal peroxidases revea ... | 2011 | 22078736 |
| fungal laccases: versatile tools for lignocellulose transformation. | conversion of lignocellulosic materials to useful, high value products normally requires a pre-treatment step to transform or deconstruct the recalcitrant and heterogeneous lignin fraction. the development of "green tools" for the transformation of lignocellulosic feedstocks is in high demand for a sustainable exploitation of such resources. this multi-faceted challenge is being addressed by an ever-increasing suite of ligninolytic enzymes isolated from various sources. among these, fungal lacca ... | 2011 | 22078735 |
| low impact strategies to improve ligninolytic enzyme production in filamentous fungi: the case of laccase in pleurotus ostreatus. | the ever-increasing demand of laccases for biodelignification, industrial oxidative processes and environmental bioremediation requires the production of large quantities of enzymes at low cost. the present work was carried out to reduce laccase production costs in liquid fermentations of the white-rot fungus pleurotus ostreatus through two different approaches. in the first, screening of fungal spent media as natural laccase inducer was performed, eliminating the presence of potentially toxic/r ... | 2011 | 22078734 |
| genomics and transcriptomics characterization of genes expressed during postharvest at 4°c by the edible basidiomycete pleurotus ostreatus. | pleurotus ostreatus is an industrially cultivated basidiomycete with nutritional and environmental applications. its genome, which was sequenced by the joint genome institute, has become a model for lignin degradation and for fungal genomics and transcriptomics studies. the complete p. ostreatus genome contains 35 mbp organized in 11 chromosomes, and two different haploid genomes have been individually sequenced. in this work, genomics and transcriptomics approaches were employed in the study of ... | 2011 | 22069155 |
| biosorption of methylene blue onto spent corncob substrate: kinetics, equilibrium and thermodynamic studies. | this study focuses on the possible use of the spent corncob substrate (scs), an agricultural waste used after the cultivation of white rot fungus pleurotus ostreatus, to adsorb methylene blue (mb) from aqueous solutions. a batch adsorption study was carried out with variable solution ph, adsorption time, temperature and initial mb concentration. it was found that mb uptake was favorable at ph ranging from 4.0 to 12.0 and the equilibrium adsorption capacity can be reached promptly within about 18 ... | 2011 | 22049698 |
| influence of pleurotus ostreatus inoculation on wood degradation and fungal colonization. | the influence of pleurotus ostreatus inoculation on wood degradation and on fungal community structure was studied. the experiments were performed on an organically poor fly ash deposit covered with a 10 cm layer of beech wood chips inoculated with p. ostreatus isolate zim76. compared to non-inoculated wood chips, inoculation increased the temperatures and relative humidities and, in the first 6 months, accelerated klason lignin degradation by 9% and also, after 17 months, increased iron translo ... | 2011 | 21963903 |
| influence of calcium and silicon supplementation into pleurotus ostreatus substrates on quality of fresh and canned mushrooms. | supplements of gypsum (calcium source), pumice (silicon source) and pumice sulfate (silicon and calcium source) into substrates for oyster mushrooms (pleurotus ostreatus) were searched for their effects on production as well as qualities of fresh and canned mushrooms. the addition of pumice up to 30% had no effect on total yield, size distribution and cap diameters. the supplementation of gypsum at 10% decreased the total yield; and although gypsum at 5% did not affect total yield, the treatment ... | 2011 | 21917642 |
| transformation of the recalcitrant pharmaceutical compound carbamazepine by pleurotus ostreatus: role of cytochrome p450 monooxygenase and manganese peroxidase. | carbamazepine (cbz) is an environmentally recalcitrant compound highly stable in soil and during wastewater treatment. in this study, we examined the mechanisms by which the white-rot fungus pleurotus ostreatus metabolizes cbz in liquid culture using a physiological approach. p. ostreatus pc9 was grown in media known to support different levels of a multiplicity of enzyme systems such as cytochrome p450 (cyp450) and manganese peroxidase (mnp). when both cyp450 and mnp systems were active, 99% of ... | 2011 | 21744850 |
| the use of a white rot fungi (pleurotus ostreatus) immobilized on amberlite xad-4 as a new biosorbent in trace metal determination. | the present work proposes the use of pleurotus ostreatus immobilized on amberlite xad-4 as new biosorbent in trace metal determination. the effects of experimental parameters, such as "ph and flow rate of sample solution, amount of solid phase, eluent type, and concentration" on the recovery of the metal ions were investigated. maximum adsorption of cr(iii), cd(ii) and cu(ii) ions took place in the ph range 4-5. these metal ions can be desorbed with 1m hcl (recovery 95-100%). 0.2g adsorbent amou ... | 2011 | 21737258 |
| agaricus blazei production on non-composted substrates based on sunflower seed hulls and spent oyster mushroom substrate. | agaricus blazei murrill is usually cultivated using the same biphasic composting method employed for a. bisporus. because cultivation of a. blazei on traditional a. bisporus composts poses some disadvantages, non-composted substrates were studied for a. blazei cultivation. mycelial growth rate and productive performance of a. blazei were evaluated on substrates containing sunflower seed hulls, pleurotus spp. spent mushroom substrate, or their combination, in the absence or in the presence of dif ... | 2011 | 25187132 |
| effect of light and reductones on differentiation of pleurotus ostreatus. | vegetative mycelia of pleurotus ostreatus were differentiated into primordia and subsequently into fruit bodies in synthetic sucrose-asparagine medium when exposed to light at low temperature. during photo-morphogenesis, l-ascorbic acid-like substances called reductones were produced. l-ascorbic acid, d-erythroascorbic acid, 5-o-(α-d-glucopyranosyl)-d-erythroascorbic acid, 5-o-(α-d-xylopyranosyl)-d-erythroascorbic acid, 5-methyl-5-o-(α-d-glucopyranosyl)-d-erythroascorbic acid and 5-methyl-5-o-(α ... | 2011 | 21369982 |
| hypolipidemic activities of dietary pleurotus ostreatus in hypercholesterolemic rats. | this work was conducted to investigate dietary supplementation of oyster mushroom fruiting bodies on biochemical and histological changes in hyper and normocholesterolemic rats. six-week old female sprague-dawley albino rats were divided into three groups of 10 rats each. feeding a diet containing a 5% powder of pleurotus ostreatus fruiting bodies to hypercholesterolemic rats reduced plasma total cholesterol, triglyceride, low-density lipoprotein (ldl), total lipid, phospholipids, and ldl/high-d ... | 2011 | 22783072 |
| application of 2iii7-3 fractional factorial experimental design to enhance enzymatic activities of pleurotus ostreatus with high concentrations of polychlorinated biphenyls. | a 2(iii)(7-3) fractional factorial experimental design was used to establish 16 culture media, with and without pcbs to enhance the activities of laccase (lac), manganese peroxidase (mnp), and versatile peroxidase (vp) produced by the white rot fungus pleurotus ostreatus. the culture was added to 10,000 mg l(-1) of transformer oil, containing 71% of the identified arochlor 1242. the culture conditions were established with eight variables at two values (levels); ph (4 and 6), agitation (100 and ... | 2011 | 21308601 |
| pleurotus ostreatus manganese-dependent peroxidase silencing impairs decolourization of orange ii. | decolourization of azo dyes by pleurotus ostreatus, a white-rot fungus capable of lignin depolymerization and mineralization, is related to the ligninolytic activity of enzymes produced by this fungus. the capacity of p. ostreatus to decolourize the azo dye orange ii (oii) was dependent and positively co-linear to mn(2+) concentration in the medium, and thus attributed to mn(2+)-dependent peroxidase (mnp) activity. based on the ongoing p. ostreatus genome deciphering project we identified at lea ... | 2010 | 21255310 |
| pleuran (β-glucan from pleurotus ostreatus) supplementation, cellular immune response and respiratory tract infections in athletes. | prolonged and exhausting physical activity causes numerous changes in immunity and sometimes transient increases the risk of upper respiratory tract infections (urtis). nutritional supplements as countermeasures to exercise-induced changes have increasingly been studied in the last decade. one of the most promising nutritional supplements is β-glucan, a well-known immunomodulator with positive effects on the function of immunocompetent cells. in this double blind, placebo-controlled study, we in ... | 2011 | 21249381 |
| effect of pleuran (β-glucan from pleurotus ostreatus) supplementation on cellular immune response after intensive exercise in elite athletes. | excessive and exhausting physical loads depress the immune system. carbohydrate consumption may minimize the postexercise suppression of the innate immune system. β-glucan is a well-known immunomodulator, with positive effects on the functioning of immunocompetent cells. the goal of this study was to determine whether β-glucan dietary supplementation from the mushroom pleurotus ostreatus decreases the suppressed immune system responses induced by short-term high-intensity exercise in humans. in ... | 2010 | 21164546 |
| a heteropolysaccharide from aqueous extract of an edible mushroom, pleurotus ostreatus cultivar: structural and biological studies. | a water soluble polysaccharide isolated from the hot aqueous extract of pleurotusostreatus cultivar was found to contain d-glucose and d-galactose in a molar ratio of nearly 7:1. structural investigation was carried out using acid hydrolysis, methylation analysis, periodate oxidation, smith degradation, and nmr studies ((1)h, (13)c, dept-135, tocsy, dqf-cosy, noesy, roesy, hmqc, and hmbc). on the basis of the above mentioned experiments the structure of the repeating unit of the polysaccharide w ... | 2011 | 21129732 |
| isolation and identification of mushroom pathogens from agrocybe aegerita. | agrocybe aegerita is an important mushroom cultivated in korea, with good feel and a peculiar fragrance. a. aegerita can be cultivated throughout the year using culture bottles but is more susceptible to contamination than other mushrooms. twenty-two pathogens were isolated from the fruiting bodies and compost of a. aegerita, and seven isolates were isolated from pleurotus ostreatus to compare with the a. aegerita isolates, collected from gimje, iksan, gunsan of chonbuk, and chilgok of gyeongbuk ... | 2010 | 23956671 |
| antioxidant activities and tyrosinase inhibitory effects of different extracts from pleurotus ostreatus fruiting bodies. | we evaluated the antioxidant activity and tyrosinase inhibitory effects of pleurotus ostreatus fruiting bodies extracted with acetone, methanol, and hot water. the antioxidant activities were tested against β-carotene-linoleic acid, reducing power, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity, and ferrous chelating ability. furthermore, phenolic acid and flavonoid contents were also analyzed. the methanol extract showed the strongest β-carotene-linoleic acid inhibition as compa ... | 2010 | 23956669 |
| identification of blue-light photoresponse genes in oyster mushroom mycelia. | using the characteristics of light-emitting diodes, we studied the effects of light quality, light intensity, and repeated light-dark cycles on the growth of oyster mushroom mycelia. blue-light stimulation suppressed growth in oyster mushroom mycelia, and this effect was remarkably dependent on light intensity. random amplified polymorphic dna differential display analysis enabled the identification for the first time of 15 upregulated and 13 downregulated genes induced by blue-light stimulation ... | 2010 | 20944395 |
| identification of a new member of pleurotus ostreatus laccase family from mature fruiting body. | laccases (benzenediol:oxygen oxidoreductases, ec 1.10.3.2) are blue multicopper oxidases, catalyzing the oxidation of an array of aromatic substrates concomitantly with the reduction of molecular oxygen to water. most of the known laccases have fungal or plant origins, although few laccases have been also identified in bacteria and insects. most of the fungal laccases reported thus far are extra-cellular enzymes, whereas only few enzymes from fruiting bodies have been described so far. multiple ... | 2010 | 20943181 |
| removal of neutral red from aqueous solution by adsorption on spent cottonseed hull substrate. | cottonseed hull, a low-cost widely available agricultural waste in china, after used as substrate for the white rot fungus pleurotus ostreatus cultivation, was tested for the removal of neutral red (nr), a cationic dye, from aqueous solution. a batch adsorption study was carried out with varied solution ph, adsorbent dosage, reaction time and initial nr concentration. the results show that the kinetics of dye removal by the spent cottonseed hull substrate (schs) is prompt in the first 5 min and ... | 2011 | 20933326 |
| induction and purification by three-phase partitioning of aryl alcohol oxidase (aao) from pleurotus ostreatus. | aryl alcohol oxidase (aao) is an extracellular flavoenzyme involved in lignin degradation by white rot fungi. screening of lignolytic and aao activity from twenty different fungal species were carried out. among them, seven species showed lignolytic activity and three of them (pleurotus ostreatus, pleurotus eous, and pleurotus platypus) were found to be aao positive. maximal aao activity was observed in batch cultures of p. ostreatus and was found to be induced by aromatic amino acids and aryl a ... | 2011 | 20857231 |
| particle geometry affects differentially substrate composition and enzyme profiles by pleurotus ostreatus growing on sugar cane bagasse. | the growth of pleurotus ostreatus was analyzed on three particle sizes of sugar cane bagasse: 0.92 mm and 1.68 mm in diameter, in addition to heterogeneous fibers (average 2.9 mm in diameter). specific growth rate on heterogeneous particles was lower (μ=0.043 h(-1)), although soluble protein production was maximal (809 μg/g dry wt). higher μ values were reached on the other two particles sizes (0.049-0.05 h(-1)) with less soluble protein (500 μg/g dry wt). xylanases and laccases were favored in ... | 2011 | 20846859 |
| increasing pleurotus ostreatus laccase production by culture medium optimization and copper/lignin synergistic induction. | laccases have great biotechnological potential in diverse industries as they catalyze the oxidation of a broad variety of chemical compounds. production of laccases by basidiomycetes has been broadly studied as they secrete the enzymes, grow on cheap substrates, and they generally produce more than one isoenzyme (constitutive and/or inducible). laccase production and isoenzyme profile can be modified through medium composition and the use of inducers. the objective of this work was to increase l ... | 2011 | 20694851 |
| antioxidant activities and total phenolic content of aqueous extract of pleurotus ostreatus (cultivated oyster mushroom). | pleurotus ostreatus better known as oyster mushroom is widely cultivated and consumed as food in malaysia. the present study aims to assess the antioxidative potential and total phenolic content of p. ostreatus aqueous extract. the antioxidant activities were evaluated against dpph and abts radical-scavenging activity, ferric-reducing antioxidant power (frap) and β-carotene-linoleate bleaching assay, and the folin-ciocalteu method for total phenolic content (tpc). the dpph and abts radical-scave ... | 2010 | 22691933 |
| differential gene expression of ligninolytic enzymes in pleurotus ostreatus grown on olive oil mill wastewater. | the expression of laccase and manganese peroxidase genes of a selected strain of pleurotus ostreatus were studied in olive oil mill wastewater (omw). the fungal strain decolourized 50% omw in a linear way for 21 days and, at the same time, degraded the phenol compounds by 85%. transcripts of laccase genes poxa1b, pox2, poxa3, and sspoxa3a, sspoxa3b coding for the small subunits of poxa3, were estimated by qrt-pcr, at different time intervals, together with beta-tubulin gene used as internal cont ... | 2010 | 20607227 |
| [comparisons of different methods for virus-elimination of edible fungi]. | four dsrna bands were extracted from pleurotus ostreatus td300 by the dsrna isolation technique with sizes of 8.2 kb, 2.5 kb, 2.1 kb, and 1.1 kb, respectively. four virus-eliminated methods, i. e. hyphal tips cut (htc), protoplast regeneration (pr), single spore hybridization (ssh), and frozen and lyophilized (fl), were applied to prepare virus-eliminated strains, and one virus-eliminated strain was selected for each virus-elimination method. the virus-eliminated strains were named as htc8, pr15 ... | 2010 | 20572349 |
| effect of steam explosion pretreatment on treatment with pleurotus ostreatus for the enzymatic hydrolysis of rice straw. | the effects of steam explosion (1.5 mpa, 1 min) on the treatment of rice straw with pleurotus ostreatus were evaluated in terms of the change in composition of the components and the susceptibility to enzymatic hydrolysis. when rice straw was pretreated with a steam explosion prior to biological treatment, the treatment time required for obtaining a 33% net glucose yield was reduced to 36 days from 60 days. the reduction is probably due to loosening of networks of klason lignin with sugar moieti ... | 2010 | 20547367 |
| differentially expressed genes under simulated microgravity in fruiting bodies of the fungus pleurotus ostreatus. | in response to a change in the direction of gravity, morphogenetic changes of fruiting bodies of fungi are usually observed as gravitropism. although gravitropism in higher fungi has been studied for over 100 years, there is no convincing evidence regarding the graviperception mechanism in mushrooms. to understand gravitropism in mushrooms, we isolated differentially expressed genes in pleurotus ostreatus (oyster mushroom) fruiting bodies developed under three-dimensional clinostat-simulated mic ... | 2010 | 20402784 |
| inoculum carrier and contaminant bioavailability affect fungal degradation performances of pah-contaminated solid matrices from a wood preservation plant. | the objective of the study was to investigate the impact of chopped wheat straw (cws), ground corn cobs (gcc) and commercial pellets (cp), as inoculum carriers, on both growth and polycyclic aromatic hydrocarbons (pah) degradation performances of dichomitus squalens, pleurotus ostreatus and coprinus comatus. a historically-contaminated soil (hcs) and creosote-treated shavings (cts) from the sobeslav wood preservation plant, characterized by different relative abundances of the pah bioavailable f ... | 2010 | 20299070 |
| the pleurotus ostreatus hydrophobin vmh2 and its interaction with glucans. | hydrophobins are small self-assembling proteins produced by fungi. a class i hydrophobin secreted by the basidiomycete fungus pleurotus ostreatus was purified and identified. the pure protein is not water soluble, whereas complexes formed between the protein and glycans, produced in culture broth containing amylose, are soluble in water. glycan structure matched to cyclic structures of alpha-(1-4) linked glucose containing from six to 16 monomers (cyclodextrins). moreover, it was verified that n ... | 2010 | 20100692 |
| bioprocess of triphenylmethane dyes decolorization by pleurotus ostreatus bp under solid-state cultivation. | with an aim to evaluate dye decolorization by white rot fungus on natural living conditions, reproducing by solidstate fermentation, the process of triphenylmethane dyes decolorization using the white rot fungus p. ostreatus bp, cultivated on rice straw solid-state medium, has been demonstrated. three typical dyes, including malachite green, bromophenol blue, and crystal violet, were almost completely decolorized by the fungus after 9 days of incubation. during the process of dye decolorization, ... | 2009 | 19996697 |
| microbial community structure changes during oyster mushroom substrate preparation. | although oyster mushroom (pleurotus spp.) is a valuable food, cultivated worldwide on an industrial scale, still very little is known about the microbial dynamics during oyster mushroom substrate preparation. therefore, the characterization of the microbial dynamics by chemical and biological tools was the objective of this study. during substrate preparation, enzymatic digestibility of the substrate improved by 77%, whereas the cellulose and hemicellulose to lignin ratios decreased by 9% and 19 ... | 2010 | 19967354 |
| purification and characteristics of an enzyme with both bilirubin oxidase and laccase activities from mycelium of the basidiomycete pleurotus ostreatus. | a homogenous enzyme with both bilirubin oxidase and laccase activities was isolated from a submerged culture of the basidiomycete pleurotus ostreatus mycelium and characterized. the yield of the enzyme was 127 microg/g dry biomass of the mycelium. the specific activity of the enzyme was 21 and 261 u/mg to bilirubin and to a laccase substrate abts, respectively. the intracellular phenol oxidase from the p. ostreatus mycelium was identified as bilirubin oxidase with the amino acid sequence highly ... | 2009 | 19916914 |
| original and residual phytotoxicity of olive mill wastewater revealed by fractionations before and after incubation with pleurotus ostreatus. | concentrations of dissolved organic carbon (doc) and total phenols (tp), and the phytotoxicity to cress (lepidium sativum l.) were determined for three molecular-sized fractions of olive mill wastewater (omw), <1000, 1000-5000, and >5000 da, before and after incubation with pleurotus ostreatus. the <1000-da fraction contained 82% of the total doc and 48% of the tp, and was the most phytotoxic. ethyl acetate separation of aqueous and solvent fractions showed that the aqueous fraction contained 93 ... | 2009 | 19904988 |
| [the composition of volatile components of dry cepe and oyster mushroom]. | the composition of aroma compounds in dry cepe mushroom (boletis edulis fr.) and oyster mushroom (pleurotus ostreatus fr.) was studied using capillary gas chromatography and chromatography-mass spectrometry. in dry cepe, 53 volatile compounds were identified, and in dry oyster mushroom 41 compounds were identified. volatile organic substances with various functional groups formed the flavor of dry mushrooms. unsaturated alcohols and ketones with eight carbon atoms were responsible for the mushro ... | 2009 | 19845295 |
| [relationship between the laccase production of pleurotus ostreatus and the full wavelength scan for the fermentation liquid]. | laccase, widely distributed in fungi lacking high substrate specificity, plays an important role in lignin degradation in nature and environmental protection. in order to determine or estimate the laccase production during the fermentation of liquid media, the authors studied the full-length wave scan on the rough fermentation liquid of the pleurotus ostreatus, which produces laccase high. combined with the normal chemical method and diameter of the laccase and mycelium stain, which grew on the ... | 2009 | 19839329 |
| development and characterization of new microsatellite markers for the oyster mushroom (pleurotus ostreatus). | we developed and characterized 36 polymorphic microsatellite markers for the oyster mushroom (pleurotus ostreatus). in total, 169 alleles were identified with an average of 4.7 alleles per locus. values for observed (ho) and expected (he) heterozygosities ranged from 0.027 to 0.946 and from 0.027 to 0.810, respectively. nineteen loci deviated from hardy-weinberg equilibrium. significant (p<0.05) excess heterozygosity was observed at nine loci. linkage disequilibrium (ld) was significant (p<0.05) ... | 2009 | 19809239 |
| biodegradation of crystal violet by low molecular mass fraction secreted by fungus. | biodegradation of crystal violet by white rot fungus pleurotus ostreatus bp in rice straw solid-state medium mostly depended on low molecular mass fraction (lmmf) and the hypothetical metabolic pathways of crystal violet by lmmf was proposed by the biodegradation of crystal violet and by the deduced six metabolites formed. | 2009 | 19804867 |
| effect of fungal pellet morphology on enzyme activities involved in phthalate degradation. | pellet size of white rot fungus, pleurotus ostreatus may affect the secretion of its degradative enzymes and accompanying biodegrading capability, but could be controlled by several physical culture conditions in liquid culture. the pellet size of p. ostreatus was affected by the volume of inoculum, flask, and medium, but the agitation speed was the most important control factor. at the lower agitation speed of 100 rpm, the large pellets were formed and the laccase activity was higher than that ... | 2009 | 19763415 |
| [determination of illudin s in omphalotus guepiniformis and foods that caused food poisoning by liquid chromatography with tandem mass spectrometry]. | a simple method was developed for determination of illudin s in fungi (omphalotus guepiniformis: poisonous mushroom) and a food that caused food poisoning, using liquid chromatography tandem mass spectrometry (lc/ms/ms). illudin s in fungi and the food that caused food poisoning was extracted with methanol and then cleaned up with an oasis hlb cartridge. lc separation was performed with an octadecylated silica column (inertsil ods-3, 2.1 mm i.d. x 150 mm) and a mobile phase of 0.1% formic acid-m ... | 2009 | 19745584 |
| random mutants of a pleurotus ostreatus laccase as new biocatalysts for industrial effluents bioremediation. | to select better performing laccase variants among the 2300 randomly mutated variants of pleurotus ostreatus poxa1b laccase to develop improved laccase-based biocatalysts. | 2010 | 19735323 |
| isolation of genes specifically expressed in different developmental stages of pleurotus ostreatus using macroarray analysis. | the oyster mushroom (pleurotus ostreatus) is one of the most important edible mushrooms worldwide. the mechanism of p. ostreatus fruiting body development has been of interest both for the basic understanding of the phenotypic change of the mycelium-fruiting body and to improve breeding of the mushrooms. based on our previous publication of p. ostreatus expressed sequence tag database, 1,528 unigene clones were used in macroarray analysis of mycelium, fruiting body and basidiospore developmental ... | 2009 | 23983539 |
| reproducible and controllable light induction of in vitro fruiting of the white-rot basidiomycete pleurotus ostreatus. | fruiting is a crucial developmental process in basidiomycetes yet the genetic and molecular factors that control it are not yet fully understood. the search for fruiting inducers is of major relevance for both basic research and for their use in industrial applications. in this paper, an efficient and reproducible protocol for controlled fruiting induction of pleurotus ostreatus growing on synthetic medium is described. the protocol is based on the control of light intensity and photoperiod and ... | 2009 | 19640403 |
| molecular characterization of the phenol oxidase (pox2) gene from the ligninolytic fungus pleurotus ostreatus. | the gene (pox2) encoding a phenol oxidase from pleurotus ostreatus, a lignin-degrading basidiomycete, was sequenced and the corresponding pox2-cdna was also synthesized, cloned and sequenced. the isolated gene consisted of 2674 bp, with the coding sequence interrupted by 19 introns and flanked by an upstream region in which the putative metal-responsive elements (mres) were determined in the promoter region (849 bp), where mre 1, 2, 3 and 4 were located in positions -20, -60, -236 and -297. a fu ... | 2009 | 19622072 |
| laccase-membrane reactors for decolorization of an acid azo dye in aqueous phase: process optimization. | in the present investigation, performance of various laccase-membrane reactor configurations including direct enzyme contact, enzyme impregnated, immobilized enzyme and a reactor system based on laccase immobilization in chitosan membranes for decolorization of azo dye (acid black 10 bx) were examined using laccase enzyme purified from white rot fungi pleurotus ostreatus 1804. a five-step laccase purification procedure was employed, which improved the enzymatic activity by 8.27 folds. laccase wa ... | 2009 | 19540548 |
| [effect of cultivation in zinc media on the growth of and the degree of zinc in organic form in transgenic metallothionein mushroom]. | metallothioneins (mts) are a family of low molecular weight, cysteine-rich and metal-binding functional proteins. transgenic mt mushroom can be used as functional food additives, but its zinc-enriching ability has not been studied systemically until now. the zinc contents in mycelia of transgenic mt mushroom (pleurotus ostreatus) and wild type mushroom mycelia cultivated in different zinc concentration media were analyzed by icp-oes. the growth status, zinc-enriching ability and degree of zinc i ... | 2009 | 19455831 |
| intracellular substrates of a heme-containing ascorbate oxidase in pleurotus ostreatus. | a novel heme-containing ascorbate oxidase isolated from oyster mushroom, pleurotus ostreatus, catalyzes oxidation of ascorbic acid (kim et al., 1996). in this report, we describe the identification of intracellular substrates of the enzyme in the mushroom. six compounds, which can serve as substrate of the heme-containing ascorbate oxidase, were identified as l-ascorbic acid, d-erythroascorbic acid, 5-o-(alpha-d-glucopyranosyl)-d-erythroascorbic acid, 5-o-(alpha-d-xylopyranosyl)-d-erythroascorbi ... | 2009 | 19412602 |
| comparative effects of oyster mushrooms on lipid profile, liver and kidney function in hypercholesterolemic rats. | comparative effects of oyster mushrooms on plasma and fecal lipid profiles and on liver and kidney function were evaluated in hyper and normocholesterolemic rats. feeding of hypercholesterolemic rats a 5% powder of oyster mushrooms (pleurotus ostreatus, p. sajor-caju and p. florida) reduced the plasma total cholesterol level by 37%, 21% and 16%, respectively and reduced the triglyceride level by 45%, 24% and 14%, respectively. ldl/hdl ratio decreased by 64%, 45% and 41% for p. sajor-caju, p. ost ... | 2009 | 23983505 |
| emulsifying agent production during pahs degradation by the white rot fungus pleurotus ostreatus d1. | for the first time the production of an emulsifying agent during phthalic, 2,2'-diphenic and alpha-hydroxy-beta-naphthoic acids, phenanthrene, anthracene, fluorene, pyrene, fluoranthene, and chrysene degradation by white rot fungus pleurotus ostreatus was found. the emulsifying activity of the cultivation medium after degradation of these compounds was assessed. maximal activities were found in the presence of chrysene (48.4%) and alpha-hydroxy-beta-naphthoic acid (52.2%). emulsifying activity i ... | 2009 | 19194747 |
| langmuir-blodgett film of hydrophobin protein from pleurotus ostreatus at the air-water interface. | we present results concerning the formation of langmuir-blodgett (lb) films of a class i hydrophobin from pleurotus ostreatus at the air-water interface, and their structure as langmuir-blodgett (lb) films when deposited on silicon substrates. lb films of the hydrophobin were investigated by atomic force microscopy (afm). we observed that the compressed film at the air-water interface exhibits a molecular depletion even at low surface pressure. in order to estimate the surface molecular concentr ... | 2008 | 18925762 |
| accumulation and localization of cesium in edible mushroom (pleurotus ostreatus) mycelia. | the characteristics of cs accumulation and localization in edible mushrooms were examined using the mycelia of pleurotus ostreatus-y1. scanning electron microscope images revealed the existence of white spots, and energy dispersive x-ray microanalyzer analysis indicated the presence of larger amounts of cs and p in these spots in mycelia cultured on medium containing 25 mm cscl. the (137)cs activities in the mycelia were approximately 4-6 times higher than those in water used for (137)cs elution ... | 2008 | 18800803 |
| a gc-ms study of the volatile organic composition of straw and oyster mushrooms during maturity and its relation to antioxidant activity. | mushrooms are very popular in the market for their nutritional and medicinal use. mushroom volatiles are not only an important factor in the flavor, but also contain many antioxidant compounds. antioxidant activity is a very important property for disease prevention. the volatile compositional characteristics of straw mushrooms (volvariella volvacea [bull. ex fr.] sing.) and oyster mushrooms (pleurotus ostreatus [jacq. ex fr.] kummer) during maturity and the mushroom antioxidant activity related ... | 2008 | 18796224 |
| the use of spent brewery grains for pleurotus ostreatus cultivation and enzyme production. | in the brewing industry, spent brewery grains (sbgs) are byproducts with a low economic value. the potential use of this leftover as a substrate ingredient for pleurotus ostreatus fruiting body cultivation and enzyme production was evaluated. the best substrate mixture for p. ostreatus mycelium growth comprised 30% wheat bran (wb), 68% beech sawdust (bs) and 2% caco3. on the substrates containing sbg, the fastest mycelium growth was observed on the substrate composed of 10% sbg, 20% wb, 68% bs a ... | 2015 | 18789406 |
| determination of the degradation products of selected sulfonated phenylazonaphthol dyes treated by white rot fungus pleurotus ostreatus by capillary electrophoresis coupled with electrospray ionization ion trap mass spectrometry. | the removal of water-soluble sulphonated phenylazonaphthol dye effluents generated by textile industries is an important issue in wastewater treatment. microbial treatment of environmental pollutants including dyes, with white rot fungi has received wide attention as a potential alternative for conventional methods in wastewater treatment. three sulphonated phenylazonaphthol dyes with similar molecular structures acid orange 7, acid orange 8 and mordant violet 5 were selected and degraded by the ... | 2008 | 18778834 |
| growth and laccase production by pleurotus ostreatus in submerged and solid-state fermentation. | pleurotus ostreatus showed atypical laccase production in submerged vs. solid-state fermentation. cultures grown in submerged fermentation produced laccase at 13,000 u l(-1), with a biomass production of 5.6 g l(-1) and four laccase isoforms. however, cultures grown in solid-state fermentation had a much lower laccase activity of 2,430 u l(-1), biomass production of 4.5 g l(-1), and three laccase isoforms. these results show that p. ostreatus performs much better in submerged fermentation than i ... | 2008 | 18762938 |
| combinations of mild physical or chemical pretreatment with biological pretreatment for enzymatic hydrolysis of rice hull. | two novel two-step pretreatments for enzymatic hydrolysis of rice hull (rh) were proposed to lower the severity requirement of fungal pretreatment time. they consisted of a mild physical or chemical step (ultrasonic and h(2)o(2)) and a subsequent biological treatment (pleurotus ostreatus). the combined pretreatments led to significant increases of the lignin degradation than those of one step pretreatments. after enzymatic hydrolysis of the pretreated rh, the net yields of total soluble sugar (t ... | 2009 | 18760920 |
| retrotransposon microsatellite amplified polymorphism strain fingerprinting markers applicable to various mushroom species. | the retrotransposon mary1 is a gypsy family retroelement, which is detected ubiquitously within the fungal taxonomic groups in which mushrooms are included. to utilize mary1 as a molecular marker for the dna fingerprinting of mushrooms, oligonucleotides mary1-ltr-l and mary1-ltr-r were designed on the basis of highly conserved regions from the multiple sequence alignment of 30 mary1 sequences retrieved from a nucleotide sequence database. in accordance with retrotransposon microsatellite amplifi ... | 2008 | 23997618 |
| fruitbody development of pleurotus ostreatus via bottle cultivation using recycled substrate. | this study was carried out to determine the possibility of bottle cultivation utilizing recycled oyster mushroom culture waste as a cultivating substrate for p. ostreatus. total nitrogen percentage was 0.76%, 1.13%, 1.16%, 1.36%, and 1.38% in the 1-, 2-, 3-, 4-, and 5-time mixed substrate, respectively; 0.95%, 1.04%, 1.34%, 1.36%, and 1.25% in the 1-, 2-, 3-, 4-, and 5-time postharvest substrate, respectively; and 0.72% and 0.68% in the 2- and 3-time nonadditive substrate, respectively. weight o ... | 2008 | 23997617 |
| manganese amendment and pleurotus ostreatus treatment to convert tomato pomace for inclusion in poultry feed. | if freshly dried tomato pomace could be fed to poultry, its naturally occurring alpha-tocopherol would retard lipid oxidation during further processing, long-term frozen storage, and heating of poultry meat; however, the high fiber content in this agricultural by-product adversely affects its use. experiments were conducted to investigate the chemical composition and in vitro true digestibility of amended (without and with 487 micromol of manganese/g) tomato pomace substrate after treatment with ... | 2008 | 18753459 |
| clinical studies on the hypolipidemic and antioxidant effects of selected natural substances. | the average blood levels of cholesterol and triacylglycerol in population are generally considered to be indicators of cardiovascular risk. | 2008 | 18700439 |
| laccases of pleurotus ostreatus observed at different phases of its growth in submerged fermentation: production of a novel laccase isoform. | the production of laccases during the lag, exponential and stationary phases of growth of pleurotus ostreatus in submerged fermentation was evaluated. laccase activity was positively correlated to the growth of the fungus. the specific growth rate was 0.02 h(-1) and the highest amount of dry biomass (7.8 gl(-1)) was obtained after 480 h of growth. four laccase isoforms were secreted by the fungus, and tentatively named l(i)1, l(i)2, l(i)3 and l(i)4. l(i)2, l(i)3 and l(i)4 were produced during th ... | 2008 | 18692377 |
| induction of fruiting in oyster mushroom (pleurotus ostreatus) by polymeric 3-alkylpyridinium salts. | polymeric 3-alkylpyridinium salts (poly-aps), surface-active compounds from the marine sponge reniera sarai, have been shown to stimulate the fruit body formation from pleurotus ostreatus mycelium. in nutrient media supplemented with poly-aps (>or= 0.01 microg ml(-1)), the formation of primordia and development of fruit bodies were detected approximately 10d earlier than in the absence of poly-aps, and also led to a considerably larger quantity of young mushrooms. this effect appears to be speci ... | 2008 | 18692375 |
| purification, structure and immunobiological activity of a water-soluble polysaccharide from the fruiting body of pleurotus ostreatus. | the water-soluble polysaccharide (pop), with a molecular mass of 2.4x10(4)da, was obtained from the fruiting body of pleurotus ostreatus. structure features of the purified polysaccharide were investigated by a combination of chemical and instrumental analysis, such as methylation analysis, smith degradation, gc-ms, (13)c and (1)h nmr and ftir. the results indicated that the backbone of pop was composed of (1-->6)-linked-alpha-d-galactopyranosyl and (1-->2,6)-linked-alpha-d-galactopyranosyl resi ... | 2009 | 18667302 |
| lignocellulolytic enzymes profile during growth and fruiting of pleurotus ostreatus on wheat straw and tree leaves. | cultivation of two commercial pleurotus ostreatus (oyster mushroom) strains was performed in plastic bags. tree leaves appeared to be an excellent growth substrate for the conversion into fruiting bodies with biological efficiency of 108-118%. the level of enzyme activity was strongly regulated during the life cycle of mushrooms. however, despite the quantitative variations, each strain had a similar pattern of enzyme accumulation in fermentation of both substrates. laccase and mnp activities we ... | 2008 | 18595320 |
| biodegradation of fluoranthene by basidiomycetes fungal isolate pleurotus ostreatus hp-1. | the biodegradation of fluoranthene, a high molecular weight polycyclic aromatic hydrocarbon (pah), was investigated in submerged culture using the wood decaying fungus isolated from forest locality in gujarat, india. the basidiomycete fungal isolate was found to have an ability to grow on sabaroud dextrose agar containing 50 mgl(-1) of each naphthalene, anthracene, acenaphthene, benzo (a) anthracene, pyrene, flouranthene, carbazole, and biphenyl. the involvement of extracellular fungal peroxidas ... | 2009 | 18574565 |
| mycelium growth and degradation of creosote-treated wood by basydiomycetes. | tolerance of wood decay fungi of the genera agrocybe, armillaria, auricularia, daedalea, pleurotus, trametes to the presence of various amounts of creosote-treated wood (ctw) in the growth medium was compared. in the case of the most tolerant strain, pleurotus ostreatus smr 684, extracellular laccase and peroxidase specific activities were monitored during growth in the presence of ctw. degradation of various creosote-constituting polycyclic aromatic hydrocarbons by this strain was evaluated by ... | 2008 | 18501950 |
| [bioremediation of oil-polluted soil with an association including the fungus pleurotus ostreatus and soil microflora]. | the possibility of application of the pleurotus ostreatus d1-soil microflora to bioremediation of oil-polluted soils was studied. the fungus degraded mainly the aromatic fraction, whereas soil microflora intensely degraded paraffin and naphthene oil fractions. introduction of the fungus pleurotus ostreatus d to soil induces degradation of a wider range of oil hydrocarbons. it is reasonable to further investigate the discovered phenomenon in order to improve procedures of remediation of oil-pollu ... | 2016 | 18491600 |
| decolorization and detoxication of reactive industrial dyes by immobilized fungi trametes pubescens and pleurotus ostreatus. | trametes pubescens and pleurotus ostreatus, immobilized on polyurethane foam cubes in bioreactors, were used to decolorize three industrial and model dyes at concentrations of 200, 1000 and 2000 ppm. five sequential cycles were run for each dye and fungus. the activity of laccase, mn-dependent and independent peroxidases, lignin peroxidase, and aryl-alcohol oxidase were daily monitored during the cycles and the toxicity of media containing 1000 and 2000 ppm of each dye was assessed by the lemna ... | 2008 | 18481217 |
| in situ recovery of the aroma compound perillene from stirred-tank cultured pleurotus ostreatus using gas stripping and adsorption on polystyrene. | supplementation of the key metabolite, alpha-(z)-acaridiol, to stirred-tank cultured pleurotus ostreatus was used to demonstrate that integrated in situ product recovery resulted in high conversion rates and quantitative separation of the target product perillene from the nutrient medium. the conversion of beta-myrcene by p. ostreatus was scaled-up from shake-flasks into a controlled, stirred tank bioreactor equipped with gas stripping and adsorption on a polystyrene fixed bed. the formation of ... | 2008 | 18414807 |
| fungal bioremediation of creosote-treated wood: a laboratory scale study on creosote components degradation by pleurotus ostreatus mycelium. | a bioremediation system for creosote-treated wood is proposed, based on the detoxifying capability of pleurotus ostreatus, a ligninolythic fungus. non-sterilized chipped contaminated wood was mixed at various ratios with wheat straw on which pleurotus mycelia was grown. at 1:2 initial ratio contaminated wood:wheat straw, chemical analyses demonstrated an almost complete degradation of creosote oil components after 44 days, also confirmed by a significant reduction of ecotoxicity. lower ratios, i ... | 2008 | 18389164 |
| the toxin produced by pleurotus ostreatus reduces the head size of nematodes. | many detrimental nematodes exist, including parasitic plant and animal nematodes. the pleurotus ostreatus (oyster mushroom) is a famous mushroom that preys upon live nematodes. however, there have been no details reported on the mechanism of this predatory activity. therefore, we investigated the predatory relationship between the nematode and p. ostreatus as a potential way of exterminating other various detrimental nematodes. upon invasion by the nematode, the mushroom defends itself by causin ... | 2008 | 18379043 |
| effect of substrate particle size and additional nitrogen source on production of lignocellulolytic enzymes by pleurotus ostreatus strains. | two strains of pleurotus ostreatus (ie-8 and cp-50) were grown on defined medium added with wheat straw extract (wse). mycelia from these cultures were used as an inoculum for solid fermentation using sugar cane bagasse (c:n=142). this substrate was used separately either as a mixture of heterogeneous particle sizes (average size 2.9 mm) or as batches with two different particle sizes (0.92 mm and 1.68 mm). protein enrichment and production of lignocellulolytic enzymes on each particle size was ... | 2008 | 18359224 |
| the identification of a novel pleurotus ostreatus dsrna virus and determination of the distribution of viruses in mushroom spores. | double-stranded rnas and virus particles were identified in pleurotus ostreatus strain shin-nong in korea. isometric virus particles with a diameter of 33 nm were purified, which are similar to other pleurotus viruses reported previously. this strain contains 5 dsrnas, 8.0, 2.5, 2.4, 2.0, and 1.8 kb in size. the virus particles contain 2 dsrnas, designated rna-1 (2.5 kb), and rna-2 (2.4 kb) which is a typical pattern of partitiviridae. a non-encapsidated dsrna of about 8.0 kb also was identified ... | 2008 | 18337700 |
| mechanism for oxidation of high-molecular-weight substrates by a fungal versatile peroxidase, mnp2. | unlike general peroxidases, pleurotus ostreatus mnp2 was reported to have a unique property of direct oxidization of high-molecular-weight compounds, such as poly r-478 and rnase a. to elucidate the mechanism for oxidation of polymeric substrates by mnp2, a series of mutant enzymes were produced by using a homologous gene expression system, and their reactivities were characterized. a mutant enzyme with an ala substituting for an exposing trp (w170a) drastically lost oxidation activity for verat ... | 2008 | 18326680 |
| biodegradation of γ-hexachlorocyclohexane (lindane) by a non-white rot fungus conidiobolus 03-1-56 isolated from litter. | biodegradation of chlorinated pesticide γ-hexachlorocyclohexane (lindane) by a nonwhite rot fungus conidiobolus 03-1-56 is reported for the first time. conidiobolus 03-1-56, a phycomyceteous fungus isolated from litter, completely degraded lindane on the 5th day of incubation in the culture medium, and gc-ecd studies confirmed that lindane removal did not occur via adsorption on the fungal biomass. degradation studies using different medium compositions showed that nitrogen/carbon limiting condi ... | 2008 | 23100707 |
| the mitochondrial genome of the basidiomycete fungus pleurotus ostreatus (oyster mushroom). | in this study, the full mitochondrial genome of a basidiomycete fungus, pleurotus ostreatus, was sequenced and analyzed. it is a circular dna molecule of 73 242 bp and contains 44 known genes encoding 18 proteins and 26 rna genes. the protein-coding genes include 14 common mitochondrial genes, one ribosomal small subunit protein 3 gene, one rna polymerase gene and two dna polymerase genes. in addition, one rna and one dna polymerase genes were identified in a mitochondrial plasmid. these two gen ... | 2008 | 18248422 |
| a labeling study on the formation of perillene by submerged cultured oyster mushroom, pleurotus ostreatus. | the conversion of beta-myrcene to the furanoid flavour compound perillene by pleurotus ostreatus was investigated using trideutero beta-myrcene, trideutero alpha-(z)-acaridiol and non-labeled 1,2- and 3,10-epoxy-beta-myrcene, alpha,alpha-acarilactol, and perillene as substrates. myrcene diols were formed from the cleavage of myrcene epoxides, but only alpha-(z)-acaridiol, a 1,4-butanediol derivative most likely generated through a base-catalysed epoxide opening, was a suitable precursor of peril ... | 2008 | 18196236 |
| development of new laccases by directed evolution: functional and computational analyses. | laccases are blue multicopper oxidases that couple the four-electron reduction of oxygen with the oxidation of a broad range of aromatic substrates. these fungal enzymes can be used for many applications such as bleaching, organic synthesis, bioremediation, and in laundry detergents. laccases from pleurotus ostreatus have been successfully heterologously expressed in yeasts. the availability of established recombinant expression systems has allowed the construction of mutated, "better performing ... | 2008 | 18186469 |
| inter-subspecies hybrid dikaryons of oyster mushroom independently isolated in vietnam and japan. | two strains of the mushroom pleurotus, isolated from nature in vietnam and japan, contained a similar combination of two distinct rdna internal transcribed spacer (its) sequences. they were perhaps hybrid dikaryons between p. cystidiosus subsp. abalonus and a novel p. cystidiosus subspecies. these mushrooms produce dikaryotic arthroconidia. this unique asexual reproduction might allow stable maintenance of a particular pair of nuclei. | 2008 | 18175897 |
| optimizing edible fungal growth and biodegradation of inedible crop residues using various cropping methods. | long-term manned space flights to mars require the development of an advanced life support (als) ecosystem including efficient food crop production, processing and recycling waste products thereof. using edible white rot fungi (ewrf) to achieve effective biomass transformation in als requires optimal and rapid biodegradative activity on lignocellulosic wastes. we investigated the mycelial growth of lentinula edodes and pleurotus ostreatus on processed residues of various crops under various crop ... | 2008 | 18155518 |
| decolorization of cotton pulp black liquor by pleurotus ostreatus in a bubble-column reactor. | decolorization of cotton pulp black liquor by pleurotus ostreatus b1 in a bubble-column reactor (bcr) was studied. the optimal conditions for the running of bcr are 30 degrees c, ph 6.0, aeration rate 1.2 l min(-1), and mycelial age 7 days. under the optimal conditions, the bcr was run for four cycles (each cycle, 12 days) and the same mycelial pellets were reused. the ultimate decolorization and cod removal rates are 76% and 80%, respectively. | 2008 | 18058052 |
| purification, characterization, and cloning of fibrinolytic metalloprotease from pleurotus ostreatus mycelia. | a fibrinolytic protease (pofe) was purified from the cultured mycelia of the edible oyster mushroom pleurotus ostreatus, using a combination of various chromatographies. the purification protocol resulted in an 876-fold purification of the enzyme, with a final yield of 6.5%. the apparent molecular mass of the purified enzyme was estimated to be 32 kda by sds-page, fibrin-zymography, and size exclusion using fplc. the optimal reaction ph value and temperature were ph 6.5 and 35 degrees c, respect ... | 2007 | 18051595 |
| genetic variability and phylogenetic relationship among proton-beam-irradiated strains of pleurotus ostreatus. | to assess the effects of a proton beam on oyster mushrooms (pleurotus ostreatus), the genetic diversity and phylogenetic relationships among strains induced by a proton beam were investigated based on a clustering analysis. according to an aflp dna polymorphism analysis, the induced strains were divided into four groups that coincided with the dose. when applying proton-beam radiation, the dissimilarity among the induced strains increased when increasing the dose. when using more than 400 gy, th ... | 2007 | 18050925 |
| decolourisation of mushroom farm wastewater by pleurotus ostreatus. | mushroom production on coffee pulp as substrate generates an intense black residual liquid, which requires suitable treatment. in the present study, pleurotus ostreatus growth in wastewater from mushroom farm was evaluated as a potential biological treatment process for decolourisation as well as to obtain biomass (liquid inoculum). culture medium components affecting mycelial growth were determined, evaluating colour removal. laccase activity was monitored during the process. p. ostreatus was a ... | 2008 | 17957486 |
| isolation of dna topoisomerase ii gene from pleurotus ostreatus and its application in phylogenetic analysis. | we performed experiments to isolate dna topoisomerase ii gene from pleurotus ostereatus and to discuss its application as a feasible and credible molecular maker in phylogenitic analysis. | 2007 | 17953613 |
| isolation of genes expressed during the developmental stages of the oyster mushroom, pleurotus ostreatus, using expressed sequence tags. | in order to isolate and identify the developmentally regulated genes during fruiting body development, cdna libraries were constructed from eight developmental stages of the oyster mushroom, pleurotus ostreatus. from these libraries, 11 761 expressed sequence tags (poests) were generated. of these, 4060 different genes (pounigenes) were identified, representing 34.5% of the entire genome. redundancy analysis of ests during the developmental stages identified eight, 13 and two genes that were spe ... | 2007 | 17937661 |
| wheat bran biodegradation by pleurotus ostreatus: a solid-state carbon-13 nmr study. | solid-state (13)c nuclear magnetic resonance (nmr) and elemental analysis techniques were used to monitor the degradation of wheat bran by the white-rot fungus pleurotus ostreatus during a 62-day cultivation period. the weight loss and in vitro organic matter digestibility of the substrate were also evaluated after fungal treatment. the (13)c nmr spectra of degraded wheat bran samples showed a lower content in carbohydrates and a higher content in aliphatic and carboxylic groups than the untreat ... | 2008 | 17920878 |
| characterization of a pleurotus ostreatus fruiting body-specific hydrophobin gene, po.hyd. | hydrophobins are a family of small, moderately hydrophobic proteins with eight cysteine residues arranged in a conserved pattern. a full-length cdna, designated po.hyd, corresponding to a hydrophobin gene of pleurotus ostreatus was obtained in our previous work. the po.hyd gene contains a 333 bp open reading frame (orf), which is interrupted by two typical classi introns. there was no consensus signal for a polya tail detected in the 3'untranslated region. however, an analogous t- or tg-rich mot ... | 2007 | 17647210 |
| hybrid production of oyster mushroom pleurotus ostreatus (jacq: fries) kummer. | optimization of industrial mushroom production depends on improving the culture process and breeding new strains with higher yields and productivities. so many works have been done on process improvement, although few systematic studies of genetic breeding of pleurotus ostreatus strains have been reported. the major aim of hybridization is to combine desirable characteristics from different strains and create variability in the existing germ plasm. in this study, we used a breeding approach to h ... | 2007 | 19070153 |