Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| functional analysis of the putative aaa atpase aipa localizing at the endocytic sites in the filamentous fungus aspergillus oryzae. | we searched for novel components involved in aspergillus oryzae endocytosis by yeast two-hybrid (yth) screening. using the endocytic marker protein aoabp1 (a. oryzae homolog of saccharomyces cerevisiae abp1p) as bait, a putative aaa (atpases associated with diverse cellular activities) atpase encoded by a gene termed aipa (aoabp1 interacting protein) was identified. further yth analyses showed that the 346-370 amino-acid region of aipa interacts with the sh3 (src homology 3) domains of aoabp1. m ... | 2011 | 21507053 |
| crystallization and diffraction analysis of β-n-acetylhexosaminidase from aspergillus oryzae. | fungal β-n-acetylhexosaminidases are enzymes that are used in the chemoenzymatic synthesis of biologically interesting oligosaccharides. the enzyme from aspergillus oryzae was produced and purified from its natural source and crystallized using the hanging-drop vapour-diffusion method. diffraction data from two crystal forms (primitive monoclinic and primitive tetragonal) were collected to resolutions of 3.2 and 2.4 å, respectively. electrophoretic and quantitative n-terminal protein-sequencing ... | 2011 | 21505251 |
| fermentation temperature affects the antioxidant activity of the enzyme-ripened sufu, an oriental traditional fermented product of soybean. | in this study, sufu, a chinese traditional fermented product of soybean, was prepared by ripening salted tofu cubes in the mash of aspergillus oryzae-fermented rice-soybean koji possessing various hydrolytic enzymes at 25°c, 37°c and 45°c. antioxidant activity including 2,2-diphenyl-2-picylhydoxyl (dpph) radical-scavenging activity, fe(2+)-chelating ability and reducing power exerted by the methanol extract of sufu was determined and compared with that of the non-fermented tofu extract. it was f ... | 2011 | 21497549 |
| identification of a major xylanase from aspergillus flavus as a 14-kd protein. | aspergillus flavus k49 secreted at least two xylanase activities when grown on a medium containing larch (wood) xylan as a sole carbon source. enzyme activity was assayed using an agar medium containing remazol brilliant blue r conjugated oat spelt xylan as substrate. crude enzyme preparations were inhibited by hg(+2), with an ed(50) of 17.5 mm and maximum inhibition of 83% at 50 mm. a concentrated sample of a. flavus k49 xylanase preparation was subjected to gel filtration chromatography on a p ... | 2011 | 21479830 |
| automatic measurement of sake fermentation kinetics using a multi-channel gas monitor system. | in small-scale sake brewing tests, progression of ethanol fermentation is examined by monitoring carbon dioxide emission using the fermograph, an automated multi-channel gas monitor instrument. the fermograph system enables automatic measurements of fermentation profiles with high accuracy, reproducibility, and resolution, and facilitates comprehensive and quantitative sake fermentation kinetic analyses. | 2011 | 21470907 |
| sake yeast strains have difficulty in entering a quiescent state after cell growth cessation. | sake yeast strains produce a high concentration of ethanol during sake brewing compared to laboratory yeast strains. as ethanol fermentation by yeast cells continues even after cell growth stops, analysis of the physiological state of the stationary phase cells is very important for understanding the mechanism of producing higher concentrations of ethanol. we compared the physiological characteristics of stationary phase cells of both sake and laboratory yeast strains in an aerobic batch culture ... | 2011 | 21459038 |
| integrated analysis of the global transcriptional response to α-amylase over-production by aspergillus oryzae. | the filamentous fungus aspergillus oryzae is an important microbial cell factory for industrial production of many enzymes, such as α-amylase. in order to optimize the industrial enzyme production process, there is a need to understand fundamental processes underlying enzyme production, here under how enzyme production links to metabolism through global regulation. through a genome-scale metabolic network for integrated analysis of transcriptome data and flux calculation, we identified key playe ... | 2011 | 21449027 |
| the a-glucuronidase agu1 from schizophyllum commune is a member of a novel glycoside hydrolase family (gh115). | schizophyllum commune produces an a-glucuronidase that is active on polymeric xylan, while the ascomycete a-glucuronidases are only active on xylan oligomers. in this study, we have identified the gene (agu1) encoding this enzyme and confirmed the functionality by overexpression of the gene in s. commune and degradation of aldopentauronic acids, (meglca)(3)-xyl(4), in the cultivation medium of the transformants. expression analysis demonstrated that agu1 is not co-regulated with the predominant ... | 2011 | 21442271 |
| immobilization of aspergillus oryzae ß galactosidase on zinc oxide nanoparticles via simple adsorption mechanism. | the present study demonstrates the immobilization of aspergillus oryzae ß galactosidase on native zinc oxide (zno) and zinc oxide nanoparticles (zno-np) by simple adsorption mechanism. the binding of enzyme on zno-np was confirmed by fourier transform-infrared spectroscopy and atomic force microscopy. native zno and zno-np showed 60% and 85% immobilization yield, respectively. soluble and immobilized enzyme preparations exhibited similar ph-optima at ph 4.5. zno-np bound ß galactosidase retained ... | 2011 | 21439994 |
| determination of the transgalactosylation activity of aspergillus oryzae +¦-galactosidase: effect of ph, temperature, and galactose and glucose concentrations. | the catalytic potential of +¦-galactosidase is usually determined by its hydrolytic activity over natural or synthetic substrates. however, this method poorly predicts enzyme behavior when transglycosylation instead of hydrolysis is being performed. a system for determining the transgalactosylation activity of +¦-galactosidase from aspergillus oryzae was developed, and its activity was determined under conditions for the synthesis of galacto-oligosaccharides and lactulose. transgalactosylation a ... | 2011 | 21439558 |
| detection of viral microrna with s1 nuclease protection assay. | mammalian host cells and their viral pathogens express and make use of short noncoding rna molecules to control the infectious cycle. in order to understand their physiological role, it is necessary to develop tools for detection and quantification of these molecules. here, we present a simple, specific, and very sensitive protocol using short radioactive dna oligonucleotides for hybridization to homologous rna target in a nuclease protection assay. the s1 nuclease from aspergillus oryzae degrad ... | 2011 | 21431685 |
| aspergillus oryzae alpha-amylase partition in potassium phosphate-polyethylene glycol aqueous two-phase systems. | the aim of this work is to study the partitioning of alpha-amylase from aspergillus oryzae in polyethylene glycol-potassium phosphate systems formed by polymers of different molecular masses with different total concentrations, several nacl concentrations and different volume ratio between the phases and at different temperatures. the enzyme was partitioned towards the top phase in the 2000-molecular-mass polyethylene glycol systems and towards the bottom phase in the other systems analyzed with ... | 2011 | 21397633 |
| effect of meat washing on the development of impact odorants in fish miso prepared from spotted mackerel. | miso, a fermented soybean paste prepared using koji (rice malt inoculated with aspergillus oryzae) has been commonly used as a traditional seasoning for several centuries in east asian countries. a miso-like fermented product was prepared using washed and unwashed meats of spotted mackerel (scomber australasicus) with improved food functionality and aroma attributes. the evolution of aroma-active volatiles was further evaluated during the early stages of maturation. | 2011 | 21384352 |
| highly efficient biodiesel production by a whole-cell biocatalyst employing a system with high lipase expression in aspergillus oryzae. | in the present study, a system with high lipase expression in aspergillus oryzae was developed using an improved enolase promoter (p-enoa124) and the 5' untranslated region of a heat-shock protein (hsp-utr). p-enoa142 enhanced the transcriptional level of a heterologous lipase gene and hsp-utr improved its translational efficiency. fusarium heterosporum lipase (fhl) was inserted into a psensu-fhl expression vector harboring p-enoa142 and hsp-utr and was transformed into an a. oryzae ns4 strain. ... | 2011 | 21380514 |
| construction and application of a functional library of cytochrome p450 monooxygenases from the filamentous fungus aspergillus oryzae. | a functional library of cytochrome p450 monooxygenases from aspergillus oryzae (aocyps) was constructed in which 121 isoforms were coexpressed with yeast nadph-cytochrome p450 oxidoreductase in saccharomyces cerevisiae. using this functional library, novel catalytic functions of aocyps, such as catalytic potentials of cyp57b3 against genistein, were elucidated for the first time. comprehensive functional screening promises rapid characterization of catalytic potentials and utility of aocyps. | 2011 | 21378053 |
| structure of a dimeric fungal a-type carbonic anhydrase. | the crystal structure of aspergillus oryzae carbonic anhydrase (aoca) was determined at 2.7å resolution and it revealed a dimer, which only has precedents in the a class in two membrane and cancer-associated enzymes. a carbonic anhydrases are underrepresented in fungi compared to the ß class, this being the first structural representative. the overall fold and zinc binding site resemble other well studied carbonic anhydrases. a major difference is that the histidine, thought to be the major prot ... | 2011 | 21377464 |
| ms/ms fragmentation-guided search of tmg-chitooligomycins and their structure-activity relationship in specific ß-n-acetylglucosaminidase inhibition. | the reducing tetrasaccharide tmg-chitotriomycin (1) is an inhibitor of ß-n-acetylglucosaminidase (glcnacase), produced by the actinomycete streptomyces anulatus nbrc13369. the inhibitor shows a unique inhibitory spectrum, that is, selectivity toward enzymes from chitin-containing organisms such as insects and fungi. nevertheless, its structure-selectivity relationship remains to be clarified. in this study, we conducted a structure-guided search of analogues of 1 in order to obtain diverse n,n,n ... | 2011 | 21373681 |
| modeling enzyme production with aspergillus oryzae in pilot scale vessels with different agitation, aeration, and agitator types. | the purpose of this article is to demonstrate how a model can be constructed such that the progress of a submerged fed-batch fermentation of a filamentous fungus can be predicted with acceptable accuracy. the studied process was enzyme production with aspergillus oryzae in 550 l pilot plant stirred tank reactors. different conditions of agitation and aeration were employed as well as two different impeller geometries. the limiting factor for the productivity was oxygen supply to the fermentation ... | 2011 | 21370231 |
| organic acids associated with saccharification of cellulosic wastes during solid-state fermentation. | saccharification of five cellulosic wastes, i.e. rice husks, wheat bran, corn cobs, wheat straw and rice straw by three cellulytic fungi, i.e. aspergillus glaums mn1, aspergillus oryzae mn2 and penicillium purpurogenum mn3, during solid-state fermentation (ssf) was laboratory studied. rice husks, wheat bran, and corn cobs were selected as inducers of glucose production in the tested fungi. an incubation interval of 10 days was optimal for glucose production. maximal activities of the cellulases ... | 2011 | 21369980 |
| identification of a new phenotype of tolerogenic human dendritic cells induced by fungal proteases from aspergillus oryzae. | we characterized a new pathway to induce tolerogenic dendritic cells (dcs) following treatment of human monocyte-derived dcs with proteases from the fungus aspergillus oryzae (asp). asp-treated dcs (asp-dcs) exhibit a cd80(-)cd83(-)cd86(-)ig-like transcript (ilt)2(-)ilt3(-)ilt4(+) phenotype, do not secrete cytokines or chemokines, and express tolerogenic markers such as glucocorticoid-induced leucine zipper, no synthetase-2, retinaldehyde dehydrogenase-1 or retinaldehyde dehydrogenase-2. when co ... | 2011 | 21368225 |
| a novel support for laccase immobilization: cellulose acetate modified with ionic liquid and application in biosensor for methyldopa detection. | a material based on cellulose acetate (ca) and the room temperature ionic liquid 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (bmi·n(tf)(2)) was developed and characterized by scanning electron microscopy, electron dispersive spectroscopy and infrared analysis. laccase (lac) from aspergillus oryzae was immobilized in this material to investigate the behavior of methyldopa by square-wave voltammetry. under optimized conditions, the lac biosensor based on ca/bmi·n(tf)(2) exhibited ... | 2011 | 21353521 |
| aflatoxin non-productivity of aspergillus oryzae caused by loss of function in the aflj gene product. | aspergillus oryzae, although closely related to aspergillus flavus, does not produce aflatoxin (af). a. oryzae rib strains can be classified into three groups (group 1-3) based on the structure of the af biosynthesis gene homolog cluster (afhc). in group 1 strains, where afhc is present, the expression level of the aflr gene is extremely low and there is no expression of the other four af homologue genes (avna, verb, omta and vbs). we conducted a detailed structural comparison of aflr orf and af ... | 2011 | 21342785 |
| impact of microencapsulated peptidase (aspergillus oryzae) on cheddar cheese proteolysis and its biologically active peptide profile. | we investigated the delivery of calcium-alginate encapsulated peptidase (flavourzyme(®), aspergillus oryzae) on proteolysis of cheddar cheese. physical and chemical characteristics such as moisture, ph and fat content were measured, and no differences were found between control and experimental cheese at day 0. sds-page analysis clearly showed that proteolysis of a and k casein was significantly accelerated after three months of maturity in the experimental cheese. a large number of low molecula ... | 2011 | 21342096 |
| autophagy delivers misfolded secretory proteins accumulated in endoplasmic reticulum to vacuoles in the filamentous fungus aspergillus oryzae. | autophagy is a conserved intracellular degradation process of eukaryotic cells. in filamentous fungi, although autophagy has been reported to have multiple physiological roles, it is not clear whether autophagy is involved in the degradation of misfolded proteins. here, we investigated the role of autophagy in the degradation of misfolded secretory proteins accumulated in endoplasmic reticulum (er) in the filamentous fungus aspergillus oryzae. in late-phase cultures, a disulfide bond-deleted mut ... | 2011 | 21334308 |
| production and characterisation of aosox2 from aspergillus oryzae, a novel flavin-dependent sulfhydryl oxidase with good ph and temperature stability. | sulfhydryl oxidases have found application in the improvement of both dairy and baking products due to their ability to oxidise thiol groups in small molecules and cysteine residues in proteins. a genome mining study of the available fungal genomes had previously been performed by our group in order to identify novel sulfhydryl oxidases suitable for industrial applications and a representative enzyme was produced, aosox1 from aspergillus oryzae (faccio et al. bmc biochem 11:31, 2010). as a resul ... | 2011 | 21327412 |
| chemical composition and antifungal activity of essential oil from cicuta virosa l. var. latisecta celak. | the essential oil extracted from the fruits of cicuta virosa l. var. latisecta celak was tested in vitro and in vivo against four foodborne fungi, aspergillus flavus, aspergillus oryzae, aspergillus niger, and alternaria alternata. forty-five different components accounting for 98.4% of the total oil composition were identified by gas chromatography-mass spectrometry. the major components were γ-terpinene (40.92%), p-cymene (27.93%), and cumin aldehyde (21.20%). antifungal activity was tested by ... | 2011 | 21320730 |
| effects of diet and aspergillus oryzae extract or saccharomyces cervisiae on growth and carcass characteristics of lambs and steers fed to meet requirements of natural markets. | two studies were conducted to determine the effects of diet and feed additive on growth and carcass characteristics of lambs and cattle destined for all natural markets. in exp. 1, 48 dorset × hampshire lambs (initial bw 29.4 ± 0.1 kg) were used in a randomized complete block experiment to determine the effects of aspergillus oryzae extract, amaferm (amf) supplementation (1 g/d) in an 85% concentrate diet on growth and carcass characteristics. lambs were allotted to 12 pens (4 lambs per pen), an ... | 2011 | 21317341 |
| disease control effect of strevertenes produced by streptomyces psammoticus against tomato fusarium wilt. | during screening of microorganisms producing antifungal metabolites, streptomyces psammoticus strain kp1404 was isolated. the culture extract of this strain showed potent disease control efficacy against fusarium wilt on tomato plants. the antifungal metabolites st-1 and st-2 were isolated from the culture extract using a variety of chromatographic procedures. on the basis of ms and nmr spectrometric analysis, the structures of the antifungal active compounds st-1 and st-2 were determined to be ... | 2011 | 21314121 |
| glutamate production from ß-glucan using endoglucanase-secreting corynebacterium glutamicum. | we demonstrate glutamate production from ß-glucan using endoglucanase (eg)-expressing corynebacterium glutamicum. the signal sequence tora derived from escherichia coli k12, which belongs to the tat pathway, was suitable for secreting eg of clostridium thermocellum using c. glutamicum as a host. using the tora signal sequence, endoglucanase from clostridium cellulovorans 743b was successfully expressed, and the secreted eg produced 123 mg of reducing sugar from 5 g of ß-glucan at 30 °c for 72 h, ... | 2011 | 21305281 |
| in situ generation of hydrogen peroxide by carbohydrate oxidase and cellobiose dehydrogenase for bleaching purposes. | the carbohydrate oxidase from microdochium nivale (caox), heterologously expressed in aspergillus oryzae, and cellobiose dehydrogenase from myriococcum thermophilum (mtcdh), were assessed for their ability to generate bleaching species at a ph suitable for liquid detergents. the substrate specificities of caox and mtcdh were analyzed on a large variety of soluble and insoluble substrates, using oxygen as an electron receptor. even insoluble substrates like cellulose were oxidized from both caox ... | 2010 | 21298807 |
| preparation of different types of miso with mixture of starters and their effects on endogenous antioxidant of liver and kidney of mice. | the present study was undertaken to evaluate the effects of feeding the male mice with miso that was prepared with a mixture of microbial starters on the level of lipid peroxidation as a marker of oxidative stress, antioxidant power of hepatocytes, the enzymatic and non-enzymatic antioxidant. the starters that were used in the preparation of miso were aspergillus oryzae and pleurotus ostreaus or a. oryzae and bacillus subtilis. the miso that was prepared with a. oryzae and bacillus subtilis has ... | 2011 | 21288263 |
| independent duplications of a-amylase in different strains of aspergillus oryzae. | aspergillus oryzae is a filamentous fungus that has arisen through the ancient domestication of aspergillus flavus for making traditional oriental foods and beverages. in the many centuries a. oryzae has been used for fermenting the starch in rice to simple sugars, it has undergone selection for increased secretion of starch-degrading enzymes. in particular, all a. oryzae strains investigated thus far have two or more copies of a gene encoding a-amylase, whereas a. flavus has only one. here we i ... | 2011 | 21277379 |
| production and partial purification of proteases from aspergillus oryzae grown in a medium based on whey protein as an exclusive nitrogen source. | several attempts have been made to incorporate whey proteins into curd to increase cheese yield. for some types of cheese, degradation of whey proteins that have been incorporated into the curd would be required to obtain acceptable flavor and texture. on the basis of the high potential for protease synthesis in aspergillus oryzae, sodium nitrate as a nitrogen source in a minimal medium for fungi, known as czapek-dox medium, was replaced with whey protein isolate to induce the protease to hydrol ... | 2011 | 21257034 |
| purification and characterization of an intracellular beta-glucosidase from the protoplast fusant of aspergillus oryzae and aspergillus niger. | protoplasts of aspergillus oryzae 3.481 and aspergillus niger 3.316 were prepared using cellulose and snail enzyme with 0.6 m nacl as osmotic stabilizer. protoplast fusion has been performed using 35% polyethylene glycol 4.000 with 0.01 mm cacl2. the fused protoplasts have been regenerated on regeneration medium and fusants were selected for further studies. an intracellular beta-glucosidase (ec 3.2.1.21) was purified from the protoplast fusant of aspergillus oryzae 3.481 and aspergillus niger 3 ... | 2010 | 21254729 |
| expression, purification and characterization of recombinant α-glucosidase in pichia pastoris. | an expression plasmid containing the agda gene encoding aspergillus oryzae zl-1 α-glucosidase was constructed and expressed in pichia pastoris x-33. the molar mass of the purified protein was estimated by sds-page. hplc analysis showed that the purified enzyme has a transglucosylating activity with maltose as substrate. the main component of the enzyme products was panose, while amounts of isomaltose and isomaltotriose were very low or absent. ph 5.2 and temperature of 37 °c were optimum for enz ... | 2010 | 21253902 |
| construction of a thiamine pyrophosphate high-producing strain of aspergillus oryzae by overexpression of three genes involved in thiamine biosynthesis. | we have found a gene (thip) encoding thiamine pyrophosphokinase (tpk) in the aspergillus oryzae genome. no riboswitch-like region was found in the upstream region of thip, although it was repressed probably by thiamine pyrophosphate (tpp) as well as thia and nmta, which are strictly regulated by tpp-riboswitch sequence. to improve the productivity of tpp in a. oryzae, we constructed the strain in which thia, nmta and thip were overexpressed simultaneously. the resulting strain accumulated intrac ... | 2011 | 21247799 |
| heterologous expression of aspergillus oryzae xylose reductase and xylitol dehydrogenase genes facilitated xylose utilization in the yeast saccharomyces cerevisiae. | the cdna encoding a putative xylose reductase (xyra) from aspergillus oryzae was cloned and coexpressed in the yeast saccharomyces cerevisiae with a. oryzae xylitol dehydrogenase cdna (xdha). xyra exhibited nadph-dependent xylose reductase activity. the s. cerevisiae strain, overexpressing the xyra, xdha, endogenous xks1, and tal1 genes, grew on xylose as sole carbon source, and produced ethanol. | 2011 | 21228482 |
| five carboxin-resistant mutants exhibited various responses to carboxin and related fungicides. | five carboxin-resistant mutants from aspergillus oryzae were characterized by the sensitivities of their mycelial growth and succinate dehydrogenase (sdh) activity to carboxin and three related fungicides. despite a significant resistance to carboxin, exhibited by all the mutants, their patterns of sensitivity to the other fungicides was distinct. this provides clues to the molecular interaction between sdh and these fungicides. | 2011 | 21228470 |
| characterization of an aspergillus oryzae cysteinyl dipeptidase expressed in escherichia coli. | cysteinyl dipeptidase from aspergillus oryzae (cdpa) was produced in escherichia coli and purified. the enzyme showed activity specific toward cysteine-containing dipeptides, but its substrate specificity was distinct from those of other cysteinyl dipeptidases of the m20 family. it was optimally active at ph 7-8 and stable at ph 6-9 and at up to 40 °c. | 2011 | 21228467 |
| post-translational his-cys cross-linkage formation in tyrosinase induced by copper(ii)-peroxo species. | autocatalytic formation of his-cys cross-linkage in the enzyme active site of tyrosinase from aspergillus oryzae has been demonstrated to proceed by the treatment of apoenzyme with cu(ii) under aerobic conditions, where a (μ-η(2):η(2)-peroxo)dicopper(ii) species has been suggested to be involved as a key reactive intermediate. | 2011 | 21218798 |
| analysis of autophagy in aspergillus oryzae by disruption of aoatg13, aoatg4, and aoatg15 genes. | autophagy is a degradation system in which cellular components are digested via vacuoles/lysosomes, and involved in differentiation in addition to helping cells to survive starvation. the autophagic process is composed of several steps: induction of autophagy, formation of autophagosomes, transportation to vacuoles, and degradation of autophagic bodies. to further understand autophagy in the filamentous fungus aspergillus oryzae, we first constructed a. oryzae mutants defective for the aoatg13, ... | 2011 | 21204928 |
| effects of moisture enhancement, enzyme treatment, and blade tenderization on the processing characteristics and tenderness of beef semimembranosus steaks. | the individual and combined effects of moisture enhancement with a salt/phosphate solution (me), blade tenderization (bt), and enzyme injection with proteinases derived from aspergillus oryzae or bacillus subtilis on cooking properties, warner-bratzler shear force (wbsf), and sensory characteristics of beef semimembranosus were investigated. me significantly (p < 0.01) reduced wbsf and increased (p < 0.05) sensory scores for juiciness and tenderness. bt increased (p < 0.05) initial and overall t ... | 2010 | 21194850 |
| loss of aspergillus oryzae amyr function indirectly affects hemicellulolytic and cellulolytic enzyme production. | aspergillus oryzae ab390, a derivative of a. oryzae or101, was found to be suitable for soy sauce production, yielding a product light brown in color. compared to the parent strain, hemicellulase and cellulase activities in the mutant were higher; however, its amylase activity was found to be much lower. to determine the cause of these differences, the enzymatic profile change, as a function of the carbon source in submerged cultures, was examined. amylase activity in ab390 was hardly detectable ... | 2010 | 21193346 |
| regioselective deglycosylation of onion quercetin glucosides by saccharomyces cerevisiae. | bioconversion of quercetin glucosides using four generally recognized as safe (gras) organisms (aspergillus oryzae, bacillus subtilis, lactobacillus plantarum, and saccharomyces cerevisiae) was evaluated by measuring changes in the levels of quercetin compounds of onion. of the four organisms, s. cerevisiae increased the content of quercetin-3-o-β-d-glucoside (iii; isoquercitrin) and quercetin (iv), whereas decreasing quercetin-3,4'-o-β-d-glucoside (i) and quercetin-4'-o-β-d-glucoside (ii). also ... | 2010 | 21188617 |
| macroautophagy-mediated degradation of whole nuclei in the filamentous fungus aspergillus oryzae. | filamentous fungi consist of continuum of multinucleate cells called hyphae, and proliferate by means of hyphal tip growth. accordingly, research interest has been focusing on hyphal tip cells, but little is known about basal cells in colony interior that do not directly contribute to proliferation. here, we show that autophagy mediates degradation of basal cell components in the filamentous fungus aspergillus oryzae. in basal cells, enhanced green fluorescent protein (egfp)-labeled peroxisomes, ... | 2010 | 21187926 |
| molecular evolution and characterization of fungal indoleamine 2,3-dioxygenases. | indoleamine 2,3-dioxygenase (ido) and tryptophan 2,3-dioxygenase (tdo) are tryptophan-degrading enzymes. mammalian ido expression is induced by cytokines and has antimicrobial and immunomodulatory effects. a major role of mammalian tdo is to supply nicotinamide adenine dinucleotide (nad(+)). in fungi, the ido homologue is thought to be expressed constitutively and supply nad(+), as tdo is absent from their genomes. here, we reveal the distribution of ido genes among fungal species and characteri ... | 2010 | 21170645 |
| integrated analysis of the global transcriptional response to α-amylase over-production by aspergillus oryzae. | the filamentous fungus aspergillus oryzae is an important microbial cell factory for industrial production of many enzymes, such as α-amylase. in order to optimize the industrial enzyme production process, there is a need to understand fundamental processes underlying enzyme production, here under how enzyme production links to metabolism through global regulation. through a genome-scale metabolic network for integrated analysis of transcriptome data and flux calculation, we identified key playe ... | 2010 | 21165907 |
| uniform culture in solid-state fermentation with fungi and its efficient enzyme production. | solid-state fermentation (ssf) has attracted a lot of interest for carrying out high-level protein production in filamentous fungi. however, it has problems such as the fermentation heat generated during the culture in addition to the reduced mobility of substances. these conditions lead to a nonuniform state in the culture substrate and result in low reproducibility. we constructed a non-airflow box (nab) with a moisture permeable fluoropolymer membrane, thereby making it possible to control an ... | 2010 | 21163699 |
| selective induction, purification and characterization of a laccase isozyme from the basidiomycete trametes sp. ah28-2. | the white-rot fungus trametes sp. ah28-2 can synthesize extracellular laccase by induction in cellobiose-based liquid culture medium. both yields and composition of laccase isozymes, produced by trametes sp. ah28-2, would be quite different with induction by different small-molecule aromatic compounds, o-toluidine, guaiacol and 3,5-dihydroxytoluene, which affected microbial growth and the synthesis of laccase isozymes differentially. higher concentrations of the three inducers could considerably ... | 2004 | 21148825 |
| production of 3,4-dihydroxy l-phenylalanine by a newly isolated aspergillus niger and parameter significance analysis by plackett-burman design. | the amino acid derivative 3,4-dihydroxy l-phenylalanine (l-dopa) is gaining interest as a drug of choice for parkinson's disease. aspergillus oryzae is commonly used for l-dopa production; however, a slower growth rate and relatively lower tyrosinase activity of mycelia have led to an increasing interest in exploiting alternative fungal cultures. in the present investigation, we report on the microbiological transformation of l-tyrosine to l-dopa accomplished by a newly isolated filamentous fung ... | 2010 | 21143944 |
| asporyergosterol, a new steroid from an algicolous isolate of aspergillus oryzae. | asporyergosterol (1), a new steroid with an e double bond between c-17 and c-20, was identified from the culture extracts of aspergillus oryzae, an endophytic fungus isolated from the marine red alga heterosiphonia japonica. moreover, four known steroids including (22e,24r)-ergosta-4,6,8(14),22-tetraen-3-one (2), (22e,24r)-3beta-hydroxyergosta-5,8,22-trien-7-one (3), (22e,24r)-ergosta-7,22-dien-3beta,5alpha,6beta-triol (4), and (22e,24r)-5alpha,8alpha-epidioxyergosta-6,22-dien-3beta-ol (5) were ... | 2010 | 21121251 |
| in situ generation of hydrogen peroxide by carbohydrate oxidase and cellobiose dehydrogenase for bleaching purposes. | the carbohydrate oxidase from microdochium nivale (caox), heterologously expressed in aspergillus oryzae, and cellobiose dehydrogenase from myriococcum thermophilum (mtcdh), were assessed for their ability to generate bleaching species at a ph suitable for liquid detergents. the substrate specificities of caox and mtcdh were analyzed on a large variety of soluble and insoluble substrates, using oxygen as an electron receptor. even insoluble substrates like cellulose were oxidized from both caox ... | 2010 | 21117085 |
| breeding and identification of novel koji molds with high activity of acid protease by genome recombination between aspergillus oryzae and aspergillus niger. | acid protease is essential for degradation of proteins during soy sauce fermentation. to breed more suitable koji molds with high activity of acid protease, interspecific genome recombination between a. oryzae and a. niger was performed. through stabilization with d-camphor and haploidization with benomyl, several stable fusants with higher activity of acid protease were obtained, showing different degrees of improvement in acid protease activity compared with the parental strain a. oryzae. in a ... | 2010 | 21107641 |
| changes in the contents of sugars and organic acids during the ripening and storage of sufu, a traditional oriental fermented product of soybean cubes. | in the present study, sufu, an oriental fermented product of soybeans, was prepared by ripening the tofu cubes in aspergillus oryzae fermented rice-soybean koji mash for 16 days at 37 °c. the sufu product was further kept at room temperature for another 30 days. examining the changes in the sugar content revealed that stachyose, raffinose, and sucrose contents of tofu and rice-soybean koji granules decreased while levels of glucose and fructose increased during the ripening period and after stor ... | 2010 | 21090680 |
| enzymes for the biofunctionalization of poly(ethylene terephthalate). | the functionalization of synthetic polymers such as poly(ethylene terephthalate) to improve their hydrophilicity can be achieved biocatalytically using hydrolytic enzymes. a number of cutinases, lipases, and esterases active on polyethylene terephthalate have been identified and characterized. enzymes from fusarium solani, thermomyces insolens, t. lanuginosus, aspergillus oryzae, pseudomonas mendocina, and thermobifida fusca have been studied in detail. thermostable biocatalysts hydrolyzing poly ... | 2010 | 21076908 |
| gc-ms based metabolite profiling of rice koji fermentation by various fungi. | in this study, aspergillus kawachii, aspergillus oryzae, and rhizopus sp., were utilized for rice koji fermentation, and the metabolites were analyzed in a time-dependent manner by gas chromatography-mass spectrometry. on principal component analysis, the metabolite patterns were clearly distinguished based on the fungi species. this approach revealed that the quantities of glucose, galactose, and glycerol gradually increased as a function of fermentation time in all trials rice koji fermentatio ... | 2010 | 21071848 |
| enzyme-mediated site-specific antibody-protein modification using a zz domain as a linker. | a zz domain (zz) and alkaline phosphatase (ap), luciferase (luc), or glucose oxidase (god) were conjugated using sortase a (srta) from staphylococcus aureus. the specific peptidyl linker for srta was genetically fused to the c-terminus of zz, and the other linker was fused to the n-terminus of ap, luc, or god, respectively. the resultant proteins were obtained separately by bacterial expression. the recombinant peptide-tagged zz and ap, luc, or god were site-specifically conjugated by srta throu ... | 2010 | 21069999 |
| heterologous expression and characterization of a glucose-stimulated β-glucosidase from the termite neotermes koshunensis in aspergillus oryzae. | neotermes koshunensis is a lower termite that secretes endogenous β-glucosidase in the salivary glands. this β-glucosidase (g1nkbg) was successfully expressed in aspergillus oryzae. g1nkbg was purified to homogeneity from the culture supernatant through ammonium sulfate precipitation and anion exchange, hydrophobic, and gel filtration chromatographies with a 48-fold increase in purity. the molecular mass of the native enzyme appeared as a single band at 60 kda after gel filtration analysis, indi ... | 2010 | 21057947 |
| extraction and application of laccases from shimeji mushrooms (pleurotus ostreatus) residues in decolourisation of reactive dyes and a comparative study using commercial laccase from aspergillus oryzae. | oxidases are able to degrade organic pollutants; however, high costs associated with biocatalysts production still hinder their use in environmental biocatalysis. our study compared the action of a commercial laccase from aspergillus oryzae and a rich extract from pleurotus ostreatus cultivation residues in decolourisation of reactive dyes: drimaren blue x-3lr (dmblr), drimaren blue x-bln (dmbbln), drimaren rubinol x-3lr (dmr), and drimaren blue c-r (rbbr). the colour removal was evaluated by co ... | 2010 | 21052547 |
| further studies of the purification and properties of the amylase of aspergillus oryzae. | 1945 | 21005743 | |
| adenosine deaminase from aspergillus oryzae. | 1946 | 20997999 | |
| screening and identification of a fungal β-glucosidase and the enzymatic synthesis of gentiooligosaccharide. | after screening with 0.1% esculoside and 0.03% fecl(3), we identified from rotten wood a fungal isolate hml0366 that produces high amount of β-glucosidase. phenotypic and rdna internal transcribed spacer sequence analyses indicated that the isolate belongs to aspergillus oryzae. the β-glucosidase produced by hml0366 had an activity of 128 u/g. high performance liquid chromatography analysis also demonstrated a high transglycosylation activity of the crude enzyme. the β-glucosidase was stable bet ... | 2010 | 20963514 |
| phylogeny of fungal hemoglobins and expression analysis of the aspergillus oryzae flavohemoglobin gene fhba during hyphal growth. | the fhba genes encoding putative flavohemoglobins (fhb) from aspergillus niger and aspergillus oryzae were isolated. comparison of the deduced amino acid sequence of the a. niger fhba gene and other putative filamentous fungal fhb-encoding genes to that of ralstonia eutropha shows an overall conserved gene structure and completely conserved catalytic amino acids. several yeasts and filamentous fungi, including both aspergillus species have been found to contain a small fhb gene family mostly con ... | 2010 | 20960969 |
| disruption of ten protease genes in the filamentous fungus aspergillus oryzae highly improves production of heterologous proteins. | proteolytic degradation by secreted proteases into the culture medium is one of the significant problems to be solved in heterologous protein production by filamentous fungi including aspergillus oryzae. double (tppa, and pepe) and quintuple (tppa, pepe, nptb, dppiv, and dppv) disruption of protease genes enhanced human lysozyme (hly) and bovine chymosin (chy) production by a. oryzae. in this study, we used a quintuple protease gene disruptant and performed successive rounds of disruption for fi ... | 2010 | 20957357 |
| in vivo imaging of endoplasmic reticulum and distribution of mutant α-amylase in aspergillus oryzae. | properly folded proteins destined for secretion exit through a specific subdomain of the endoplasmic reticulum (er) known as transitional er (ter) sites or er exit sites (eres). while such proteins in filamentous fungi localize at the hyphal tips overlapping the spitzenkörper, the distribution of misfolded proteins remains unknown. in the present study, we analyzed the distribution of mutant protein as well as er and ter sites visualized by expression of aoclxa and aosec13 fused with fluorescent ... | 2010 | 20884367 |
| enzyme stabilization and immobilization by sol-gel entrapment. | while biocatalysts show tremendous potential for the industrial production of fine chemicals, their integration into large-scale processes has been slow. one of the main reasons for slow acceptance in industry is the inherent instability of the enzymes. recent developments in bioengineering have shed some light on methods of improving enzyme stability. one method that has been used for many decades, successfully to varying degrees, has been the immobilization of enzymes. to this regards, silica ... | 2011 | 20865388 |
| identification and characterization of a novel fermented substance produced by edible aspergillus oryzae ao-1 that inhibits dpp-iv activity. | we identified a novel fermented substance (fka) produced by the edible aspergillus oryzae strain ao-1 that inhibited dipeptidyl peptidase iv (dpp-iv) (ic(50) = 3.41 mg · ml(-1)). hplc analysis of fka showed specific one metabolite (wyk-1), which inhibited dpp-iv (ic(50) = 6.98 μm). wyk-1 was identified as a tetrahydroxyisoquinoline derivative. interestingly, we examined 60 strains of a. oryzae, ao-1 was the only a. oryzae strain that produces wyk-1. this is the first report that an edible a. ory ... | 2011 | 20851672 |
| identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from aspergillus oryzae. | kojic acid is produced in large amounts by aspergillus oryzae as a secondary metabolite and is widely used in the cosmetic industry. glucose can be converted to kojic acid, perhaps by only a few steps, but no genes for the conversion have thus far been revealed. using a dna microarray, gene expression profiles under three pairs of conditions significantly affecting kojic acid production were compared. all genes were ranked using an index parameter reflecting both high amounts of transcription an ... | 2010 | 20849972 |
| crystallization and preliminary x-ray analysis of formate oxidase, an enzyme of the glucose-methanol-choline oxidoreductase family. | formate oxidase (fod), which catalyzes the oxidation of formate to yield carbon dioxide and hydrogen peroxide, belongs to the glucose-methanol-choline oxidoreductase (gmco) family. fod from aspergillus oryzae rib40, which has a modified fad as a cofactor, was crystallized at 293 k by the hanging-drop vapour-diffusion method. the crystal was orthorhombic and belonged to space group c222(1). diffraction data were collected from a single crystal to 2.4 a resolution. | 2010 | 20823527 |
| functional analysis and subcellular location of two flavohemoglobins from aspergillus oryzae. | multiple flavohemoglobin (fhb) homolog genes are found in the genomes of eukaryotic microorganisms, but their functions remain unknown. in this study, two distinct types of fhbs (predictive cytosolic fhb1 and predictive mitochondrial fhb2) from the fungus aspergillus oryzae were investigated to elucidate the physiological roles of these fhbs. the fhb1 gene responded to external nitric oxide (no) stress at the transcriptional level, whereas the fhb2 gene did not. disrupting fhb1 increased cell hy ... | 2011 | 20817113 |
| overexpression and characterization of an extracellular leucine aminopeptidase from aspergillus oryzae. | leucine aminopeptidase (lap), an enzyme used in the food industry, is an exopeptidase that removes an amino acid residue, primarily leucine (leu), from the n-terminus of peptides and protein substrates. in this study, we focused on the leucine aminopeptidase a (lapa) gene from aspergillus oryzae rib40. to purify and characterize the lapa, lapa was overexpressed in a. oryzae rib40 using the amyb promoter. lap activity in the culture supernatant of one transformant harboring the lapa expression pl ... | 2011 | 20803144 |
| indoloditerpenes from an algicolous isolate of aspergillus oryzae. | two new indoloditerpene derivatives asporyzin a (1) and asporyzin b (2), one new indoloditerpene asporyzin c (3), and three known related indoloditerpenes jbir-03 (4), emindole sb (5), and emeniveol (6) were isolated from an endophytic fungus aspergillus oryzae, isolated from the marine red alga heterosiphonia japonica. their structures were unambiguously established by spectroscopic techniques. in addition, all the isolates were evaluated preliminarily for insecticidal and antimicrobial activit ... | 2010 | 20797856 |
| a novel aspergillus oryzae esterase that hydrolyzes 4-hydroxybenzoic acid esters. | in this study we report the biochemical characterization of a hypothetical protein from aspergillus oryzae exhibiting sequence identity with feruloyl esterase and tannase from the genus aspergillus. the purified recombinant protein showed a hydrolytic activity toward the ethyl, propyl, or butyl esters of 4-hydroxybenzoic acid, but did not show feruloyl esterase or tannase activity. finally, the enzyme decreased the antimicrobial activity of parabens against a. oryzae via hydrolysis of the ester ... | 2010 | 20728445 |
| contribution of ethanol-tolerant xylanase g2 from aspergillus oryzae on japanese sake brewing. | we purified three xylanase isozymes (xynf1, xynf3 and xyng2) from a solid-state aspergillus oryzae rib128 culture using chromatography. the results of our sake-brewing experiment, in which we used exogenously supplemented enzymes, revealed that only xyng2 improved the alcohol yield and the material utilization. the alcohol yield of the xyng2 batch displayed an increase of 4.4% in comparison to the control, and the amount of sake cake decreased by 4.6%. the contribution of xyng2 was further confi ... | 2010 | 20727822 |
| secreted fungal sulfhydryl oxidases: sequence analysis and characterisation of a representative flavin-dependent enzyme from aspergillus oryzae. | sulfhydryl oxidases are flavin-dependent enzymes that catalyse the formation of de novo disulfide bonds from free thiol groups, with the reduction of molecular oxygen to hydrogen peroxide. sulfhydryl oxidases have been investigated in the food industry to remove the burnt flavour of ultraheat-treated milk and are currently studied as potential crosslinking enzymes, aiming at strengthening wheat dough and improving the overall bread quality. | 2010 | 20727152 |
| three-dimensional phase-contrast imaging by a computed-tomography microscope. | we report a technique for measuring the three-dimensional variation of refractive indices in a microscopic sample. the technique is an adaptation of optical computed tomography and is effective in measuring the three-dimensional refractive-index distribution of a nonabsorbing microscopic sample. our report also includes a discussion of the conditions for the unambiguous application of the technique as well as results of experiments conducted with aspergillus oryzae (commonly known as green mold) ... | 1992 | 20720664 |
| characterization of a cellobiohydrolase (mocel6a) produced by magnaporthe oryzae. | three gh-6 family cellobiohydrolases are expected in the genome of magnaporthe grisea based on the complete genome sequence. here, we demonstrate the properties, kinetics, and substrate specificities of a magnaporthe oryzae gh-6 family cellobiohydrolase (mocel6a). in addition, the effect of cellobiose on mocel6a activity was also investigated. mocel6a contiguously fused to a histidine tag was overexpressed in m. oryzae and purified by affinity chromatography. mocel6a showed higher hydrolytic act ... | 2010 | 20709852 |
| purification and characterization of termite endogenous beta-1,4-endoglucanases produced in aspergillus oryzae. | although termites are known to have a highly efficient lignocellulose-digesting system, mass production of native endogenous cellulases of termites has failed in escherchia coli, and in saccharomyces cerevisiae, and it has not been accomplished. here we report the successful production, purification, and characterization of two termite endogenous beta-1,4-endoglucanases, rseg and nteg, from the salivary gland of reticulitermes speratus and the midgut of nasutitermes takasagoensis respectively, u ... | 2010 | 20699551 |
| enzymatic synthesis and characterization of hydroquinone galactoside using kluyveromyces lactis lactase. | hydroquinone galactoside (hq-gal) as a potential skin whitening agent was synthesized by the reaction of lactase (beta-galactosidase) from kluyveromyces lactis, aspergillus oryzae, bacillus circulans, and thermus sp. with lactose as a donor and hq as an acceptor. among these lactases, the acceptor reaction involving hq and lactose with k. lactis lactase showed a higher conversion ratio to hq-gal (60.27%). hq-gal was purified using butanol partitioning and silica gel column chromatography. the st ... | 2010 | 20687552 |
| simple metabolite extraction method for metabolic profiling of the solid-state fermentation of aspergillus oryzae. | solid-state culture of microorganisms is an important style of culture both in the traditional food industry and in the modern fermentation industry. we propose here a simple method for metabolite extraction from the solid-state fermentation of a filamentous fungus, aspergillus oryzae, which is known as rice-koji. to evaluate the efficiency of metabolite extraction, liquid chromatography-mass spectrometry (lc/ms) was used for simultaneous detection of a wide range of metabolites including amino ... | 2010 | 20685162 |
| a comparative study of activity and apparent inhibition of fungal β-glucosidases. | β-glucosidases (bgs) from aspergillus fumigates, aspergillus niger, aspergillus oryzae, chaetomium globosum, emericella nidulans, magnaporthe grisea, neurospora crassa, and penicillium brasilianum were purified to homogeneity, and analyzed by isothermal titration calorimetry with respect to their hydrolytic activity and its sensitivity to glucose (product) using cellobiose as substrate. global non-linear regression of several reactions, with or without added glucose, to a product inhibition equa ... | 2010 | 20677177 |
| a novel enzymatic microreactor with aspergillus oryzae β-galactosidase immobilized on silicon dioxide nanosprings. | the use of silicon dioxide (sio(2) ) nanosprings as supports for immobilized enzymes in a continuous microreactor is described. a nanospring mat (2.2 cm(2) × 60 μm thick) was functionalized with γ-aminopropyltriethoxysilane, then treated with n-succinimidyl-3-(2-pyridyldithio)-propionate (spdp) and dithiothreitol (dtt) to produce surface thiol (--sh) groups. spdp-modified β-galactosidase from aspergillus oryzae was immobilized on the thiolated nanosprings by reversible disulfide linkages. the en ... | 2010 | 20661927 |
| microbiological and biochemical survey on the transition of fermentative processes in fukuyama pot vinegar brewing. | traditional brewing of fukuyama pot vinegar is a process that has been continued in fukuyama, kagoshima, japan, for almost 200 years. the entire process proceeds from raw materials, including steamed rice, rice koji (steamed rice grown with a fungus, aspergillus oryzae) and water, to produce vinegar in roughly capped large pots laid in the open air. no special fermentative manipulation is required, except for scattering dried rice koji (called furi-koji) on the surface of the mash to form a cap- ... | 2010 | 20647677 |
| study on the synthesis of sucrose-6-acetate catalyzed by fructosyltransferase from aspergillus oryzae. | the study had mainly investigated the synthesis of sucrose-6-acetate (s-6-a) in fructosyltransferase action. the synthesis reaction of s-6-a was performed between sucrose and glucose-6-acetate (g-6-a), and identified by high performance liquid chromatography (hplc). according to the reaction of s-6-a catalyzed by fructosyltransferase from aspergillus oryzae, the effect factors of reaction, such as the ratio of g-6-a to sucrose, temperature, time, ph, substrate and enzyme concentration in the rea ... | 2011 | 20643232 |
| aspergillus oryzae type iii polyketide synthase csya is involved in the biosynthesis of 3,5-dihydroxybenzoic acid. | as a novel superfamily of type iii polyketide synthases in microbes, four genes csya, csyb, csyc, and csyd, were found in the genome of aspergillus oryzae, an industrially important filamentous fungus. in order to analyze their functions, we carried out the overexpression of csya under the control of alpha-amylase promoter in a. oryzae and identified 3,5-dihydroxybenzoic acid (dhba) as the major product. feeding experiments using (13)c-labeled acetates confirmed that the acetate labeling pattern ... | 2010 | 20630753 |
| enhanced production and secretion of heterologous proteins by the filamentous fungus aspergillus oryzae via disruption of vacuolar protein sorting receptor gene aovps10. | filamentous fungi have received attention as hosts for heterologous protein production because of their high secretion capability and eukaryotic posttranslational modifications. however, despite these positive attributes, a bottleneck in posttranscriptional processing limits protein yields. the vacuolar protein sorting gene vps10 encodes a sorting receptor for the recognition and delivery of several yeast vacuolar proteins. although it can also target recombinant and aberrant proteins for vacuol ... | 2010 | 20622126 |
| a novel pathway for nicotine degradation by aspergillus oryzae 112822 isolated from tobacco leaves. | an efficient nicotine-degrading fungus was isolated from tobacco leaves and identified as aspergillus oryzae 112822 based on morphological characteristics and sequence analysis of 18s rdna, 5.8s rdna and the internal transcribed spacer (5.8s-its region). when the strain was cultured in a medium with tobacco leaf extract for 40 h, the maximum amount of cell growth was 3.6 g l(-1) and nicotine degradation was 2.19 g l(-1). the intermediates of nicotine degradation by resting cells were isolated by ... | 2010 | 20600861 |
| identifying 8-hydroxynaringenin as a suicide substrate of mushroom tyrosinase. | a biotransformed metabolite of naringenin was isolated from the fermentation broth of aspergillus oryzae, fed with naringenin, and identified as 8-hydroxynaringenin based on the mass and (1)h- and (13)c-nmr spectral data. the compound showed characteristics of both an irreversible inhibitor and a substrate of mushroom tyrosinase in preincubation and hplc analysis. these results demonstrate that 8-hydroxynaringenin belongs to a suicide substrate of mushroom tyrosinase. the partition ratio between ... | 2010 | 20587349 |
| first heterologous reconstruction of a complete functional fungal biosynthetic multigene cluster. | 2010 | 20575135 | |
| applicability of penicillium chrysogenum rheological correlations to broths of other fungal strains. | existing correlations of power law consistency index with penicillium chrysogenum biomass concentration and morphology were revised using a microscope magnification of 50 times to characterize the latter, rather than the 80 times used previously. this allowed tests of the correlations on broths of aspergillus oryzae and aspergillus niger, which have such large mycelial sizes that a lower magnification is required for accurate morphological analysis. the new correlations were successful at predic ... | 2010 | 20574833 |
| gene cloning and enzyme structure modeling of the aspergillus oryzae n74 fructosyltransferase. | the fructooligosaccharides (fos) represent an important source of prebiotic compounds that are widely used as an ingredient in functional foods. recently, the strain aspergillus oryzae n74 was reported as a potential microorganism for the industrial production of fos, due to its high yields of fos production. in this work, we used a pcr-cloning strategy to clone the a. oryzae n74 ftase gene as a previous step for recombinant enzyme production. ftase showed a 1630 bp size with a 99% similarity wi ... | 2011 | 20563857 |
| alternative method for quantification of alfa-amylase activity. | a modification of the sensitive agar diffusion method was developed for macro-scale determination of alfa-amylase. the proposed modifications lower costs with the utilisation of starch as substrate and agar as supporting medium. thus, a standard curve was built using alfa-amylase solution from aspergillus oryzae, with concentrations ranging from 2.4 to 7,500 u.ml-1. clear radial diffusion zones were measured after 4 hours of incubation at 20 a degrees c. a linear relationship between the logarit ... | 2010 | 20552148 |
| penicillin biosynthesis in aspergillus oryzae and its overproduction by genetic engineering. | aspergillus oryzae penicillin biosynthetic genes were clustered. the penicillin production was positively regulated by vea, a global gene regulator required for transcriptional expression of the penicillin biosynthetic genes. overexpression of the biosynthetic genes by a strong promoter yielded a greater than 100-fold increase in penicillin production. | 2010 | 20541108 |
| expression and export: recombinant protein production systems for aspergillus. | several aspergillus species, in particular aspergillus niger and aspergillus oryzae, are widely used as protein production hosts in various biotechnological applications. in order to improve the expression and secretion of recombinant proteins in these filamentous fungi, several novel genetic engineering strategies have been developed in recent years. this review describes state-of-the-art genetic manipulation technologies used for strain improvement, as well as recent advances in designing the ... | 2010 | 20532762 |
| uptake of glycosylated ?-galactosidase into human brain synaptosomes: occurrence of two different systems for specific glycosides. | uptake of glycoproteins into brain nerve terminals was studied, using glycosylated ?-galactosidase and human brain synaptosomes. a series of p-aminophenyl ?- or ?-glycosides were covalently bound to ?-galactosidase purified from aspergillus oryzae and uptake of the enzyme into human brain synaptosomes was measured. ?-galactosidase coupled with ?-d-mannoside (?-d-man ?-gal) was the best to be taken up among glycoconjugates tested. the uptake was dependent on the amount of ?-d-man ?-gal, the incub ... | 1988 | 20501274 |
| a randomized controlled trial to assess the impact of dietary energy sources, feed supplements, and the presence of super-shedders on the detection of escherichia coli o157:h7 in feedlot cattle using different diagnostic procedures. | alteration of the gastro-intestinal tract through manipulation of cattle diets has been proposed as a preharvest control measure to reduce fecal shedding of escherichia coli o157:h7. the objective of this study was to examine the effects of the energy source's moisture content (high moisture corn and dry whole-shelled corn), two natural feed supplements (saccaromyces cerevisiae boulardii cncm 1079-levucell and aspergillus oryzae-amaferm), and two levels of vitamin a (2200 iu/kg and no supplement ... | 2010 | 20500082 |
| display of both n- and c-terminal target fusion proteins on the aspergillus oryzae cell surface using a chitin-binding module. | a novel cell surface display system in aspergillus oryzae was established by using a chitin-binding module (cbm) from saccharomyces cerevisiae as an anchor protein. cbm was fused to the n or c terminus of green fluorescent protein (gfp) and the fusion proteins (gfp-cbm and cbm-gfp) were expressed using a. oryzae as a host. western blotting and fluorescence microscopy analysis showed that both gfp-cbm and cbm-gfp were successfully expressed on the cell surface. in addition, cell surface display o ... | 2010 | 20499230 |
| solanapyrone synthase, a possible diels-alderase and iterative type i polyketide synthase encoded in a biosynthetic gene cluster from alternaria solani. | the solanapyrone biosynthetic gene cluster was cloned from alternaria solani. it consists of six genes-sol1-6-coding for a polyketide synthase, an o-methyltransferase, a dehydrogenase, a transcription factor, a flavin-dependent oxidase, and cytochrome p450. the prosolanapyrone synthase (pss) encoded by sol1 was expressed in aspergillus oryzae and its product was identified as desmethylprosolanapyrone i (8). although pss is closely related to the pkss/diels-alderases lovb and mlca of lovastatin a ... | 2010 | 20486243 |
| antimicrobial and antioxidant activities of the essential oil from herba moslae. | herba moslae is a well-known edible and medicinal plant in china, and the essential oil, which is assumed to contain the active components, was isolated by steam distillation method. the aim of this study was to investigate the antimicrobial activity and the antioxidant activity of the essential oil from herba moslae. | 2010 | 20474054 |
| simultaneous c7- and n1-prenylation of cyclo-l-trp-l-trp catalyzed by a prenyltransferase from aspergillus oryzae. | a putative prenyltransferase gene ctrppt was amplified from aspergillus oryzae dsm1147, cloned into pqe70 and overexpressed in escherichia coli. the overproduced his(6)-ctrppt was purified to near homogeneity and incubated with l-tryptophan or tryptophan-containing cyclic dipeptides in the presence of dimethylallyl diphosphate. the formation of the enzyme products was monitored with hplc. it was shown that ctrppt differed clearly from other known indole prenyltransferases in several aspects. thi ... | 2010 | 20473424 |