Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| optimisation of soy flour fermentation parameters to produce β-glucosidase for bioconversion into aglycones. | the solid state fermentation (ssf) parameters of defatted soybean flour (dsf) with aspergillus oryzae ioc 3999/1998 or monascus purpureus nrrl 1992 was evaluated using a rotational central composite experimental design to optimise the production of β-glucosidase and convert glycosidic isoflavones in aglycones. variables investigated were initial ph of dsf, volume of water added to 10 g of dsf and incubation temperature. β-glucosidase activity was measured using the synthetic substrate, p-nitroph ... | 2014 | 24444906 |
| high level production of β-galactosidase exhibiting excellent milk-lactose degradation ability from aspergillus oryzae by codon and fermentation optimization. | a β-galactosidase gene from aspergillus oryzae was engineered utilizing codon usage optimization to be constitutively and highly expressed in the pichia pastoris smd1168h strain in a high-cell-density fermentation. after fermentation for 96 h in a 50-l fermentor using glucose and glycerol as combined carbon sources, the recombinant enzyme in the culture supernatant had an activity of 4,239.07 u ml(-1) with o-nitrophenyl-β-d-galactopyranoside as the substrate, and produced a total of extracellula ... | 2014 | 24435763 |
| a promiscuous prenyltransferase from aspergillus oryzae catalyses c-prenylations of hydroxynaphthalenes in the presence of different prenyl donors. | prenyltransferases of the dimethylallyltryptophan synthase (dmats) superfamily are involved in the biosynthesis of secondary metabolites and show broad substrate specificity towards their aromatic substrates with a high regioselectivity for the prenylation reactions. most members of this superfamily accepted as prenyl donor exclusively dimethylallyl diphosphate (dmapp). one enzyme, anapt from neosartorya fischeri, was reported recently to use both dmapp and geranyl diphosphate (gpp) as prenyl do ... | 2014 | 24430210 |
| comparative analysis of aspergillus oryzae with normal and abnormal color conidia. | this study focuses on the characteristic of strains with anomalous color conidium and compares with normal color conidium. comparative analysis of enzymes activity and extracellular proteins revealed that a. oryzae with anomalous color conidium was not different from the strain with normal color conidium. in addition, a. oryzae with anomalous color conidium could not influence the palatability and quality of the soy sauce. these findings provide an insight into a. oryzae with anomalous color con ... | 2014 | 24426175 |
| hydrolysis of green tea residue protein using proteolytic enzyme derived from aspergillus oryzae. | free amino acids are important chemical components which impact the taste of green tea infusion. the hydrolysis of water-insoluble protein in the green tea residue helps to increase the contents of free amino acids components except theanine. studies indicate that the hydrolysis of the tea protein could be restricted due to interaction of polyphenols with protein. the experiment indicates that the hydrolysis of tea protein by protease is the main trend when the polyphenols concentration is lower ... | 2013 | 24425904 |
| increasing glutaminase activity in shoyu koji using a mixed culture of two koji moulds. | for improved fermentation of shoyu (soy sauce), a useful koji-making system has been developed using a mixed tane-koji of two shoyu koji moulds, namely aspergillus oryzae k2 (length of conidiophores about 350 μm) and the late-conidiation strain, a. oryzae hg (length of conidiophores about 2500 μm). the mixed culture of strains k2 and hg had about twice the glutaminase activity of the single-strain cultures. in addition, the number of conidia in the mixed culture was about 10% of that in a cultur ... | 1994 | 24421198 |
| the effect of urea on growth of moulds and biomass yield in solid-state cultivation. | urea, added at 2 to 20 mg/g in solid bran medium supporting growth of aspergillus oryzae and rhizopus oligosporus, led to a higher concentration of nh4 (+) and ph but a decrease in biomass production. | 1995 | 24414664 |
| physiological characterization of the high malic acid-producing aspergillus oryzae strain 2103a-68. | malic acid is a c₄ dicarboxylic acid that is currently mainly used in the food and beverages industry as an acidulant. because of the versatility of the group of c₄ dicarboxylic acids, the chemical industry has a growing interest in this chemical compound. as malic acid will be considered as a bulk chemical, microbial production requires organisms that sustain high rates, yields, and titers. aspergillus oryzae is mainly known as an industrial enzyme producer, but it was also shown that it has a ... | 2014 | 24413918 |
| quantitation and structural determination of glucosylceramides contained in sake lees. | sake lees are solid parts filtered from the mash of sake, the traditional rice wine of japan, which is brewed with aspergillus oryzae and saccharomyces cerevisiae. the moisture-holding activity of sake lees has long been recognized in japan. however, the constituent responsible for this activity has not been elucidated. in this study, we first determined the structure of the glucosylceramides contained in sake lees. the glucosylceramides contained in sake lees were n-2'-hydroxyoctadecanoyl-l-o-β ... | 2014 | 24389795 |
| development of a pyrg mutant of aspergillus oryzae strain s1 as a host for the production of heterologous proteins. | the ease with which auxotrophic strains and genes that complement them can be manipulated, as well as the stability of auxotrophic selection systems, are amongst the advantages of using auxotrophic markers to produce heterologous proteins. most auxotrophic markers in aspergillus oryzae originate from chemical or physical mutagenesis that may yield undesirable mutations along with the mutation of interest. an auxotrophic a. oryzae strain s1 was generated by deleting the orotidine-5'-monophosphate ... | 2013 | 24381522 |
| a novel method for beef potentiator preparation and identification of its characteristic aroma compounds. | beef potentiator (bp) is the most popular savoury flavour and regarded as the soul of the modern food industry. in this work, bp was prepared by a novel method with aspergillus oryzae and aspergillus niger (bpsf). three other bps prepared using commercial enzymes (protamex, flavourzyme and papain; bpces) were used as controls to investigate its aroma characteristics and related compounds. | 2014 | 24374657 |
| discovery and characterization of a new family of lytic polysaccharide monooxygenases. | lytic polysaccharide monooxygenases (lpmos) are a recently discovered class of enzymes capable of oxidizing recalcitrant polysaccharides. they are attracting considerable attention owing to their potential use in biomass conversion, notably in the production of biofuels. previous studies have identified two discrete sequence-based families of these enzymes termed aa9 (formerly gh61) and aa10 (formerly cbm33). here, we report the discovery of a third family of lpmos. using a chitin-degrading exem ... | 2014 | 24362702 |
| enzymatic transformation of polydatin to resveratrol by piceid-β-d-glucosidase from aspergillus oryzae. | resveratrol is now gaining much attention because of its pharmacological properties. polygonum cuspidatum has the highest content of resveratrol in plants and is the best material offering resveratrol. however, the content of resveratrol in p. cuspidatum is much lower compared with its glycoside polydatin. in this study, enzymatic transformation of polydatin to resveratrol by piceid-β-d-glucosidase from aspergillus oryzae sp. 100 was investigated. the biotransformation conditions were optimized. ... | 2014 | 24362562 |
| sequence analysis and heterologous expression of the wool cuticle-degrading enzyme encoding genes in fusarium oxysporum 26-1. | two protease-like proteins, krta and krtc, were identified in fusarium oxysporum 26-1. genes coding these proteins, krta and krtc, were isolated and characterized. recombinant krta (rkrta) and krtc (rkrtc) were successfully expressed in aspergillus oryzae and secreted. the combination of rkrta and rkrtc completely removed the cuticle of wool fibers. | 2014 | 24360406 |
| a comparative study of extraction techniques for maximum recovery of glutamate decarboxylase (gad) from aspergillus oryzae nsk. | γ-amino butyric acid (gaba) is a major inhibitory neurotransmitter of the mammalian central nervous system that plays a vital role in regulating vital neurological functions. the enzyme responsible for producing gaba is glutamate decarboxylase (gad), an intracellular enzyme that both food and pharmaceutical industries are currently using as the major catalyst in trial biotransformation process of gaba. we have successfully isolated a novel strain of aspergillus oryzae nsk that possesses a relati ... | 2013 | 24321181 |
| production optimization and expression of pectin releasing enzyme from aspergillus oryzae po. | protopectinase is an enzyme that solubilizes protopectin forming highly polymerized soluble pectin. protopectinase activity was detected from aspergillus oryzae po isolated from soil of persimmon orchard. response surface methodology of box-behnken design with three fermentation variables (temperature, nano3 and apple pomace concentration) was used to optimize protopectinase production of a. oryzae po, and protopectinase activity was improved to 270.0 u/ml. endo-polygalacturonase belonged to a-t ... | 2014 | 24299753 |
| the henry reaction in [bmim][pf₆]-based microemulsions promoted by acylase. | an environmentally-friendly, enzyme-promoted procedure for the henry reaction was first studied using water-in-[bmim][pf6] microemulsions as reaction medium. the amano acylase from aspergillus oryzae showed better catalytic activity for the addition reactions of nitromethane with a series of aromatic aldehydes, and a highest yield of 90% was obtained. | 2013 | 24284489 |
| enhanced β-galactosidase production of aspergillus oryzae mutated by uv and licl. | in order to breed a high-yield β-galactosidase-producing strain, aspergillus oryzae was used as the parent strain and mutagenized with ultraviolet (uv) and uv plus lithium chloride (licl), respectively. after being mutagenized by uv, the β-galactosidase activity of mutant uv-15-20 reached 114.08 u/ml, which revealed a 49.22% increase compared with the original strain. a mutant uv-licl-38 with high β-galactosidase activity (121.42 u/ml) was obtained after compound mutagenesis of uv and licl; the ... | 2014 | 24274018 |
| deglycosylation of isoflavones in isoflavone-rich soy germ flour by aspergillus oryzae kacc 40247. | aspergillus oryzae kacc 40247 was selected as an efficient daidzein-producing fungus from strains of the genus aspergillus by using 5% (w/v) soy germ flour (sgf) as an isoflavone-glycoside-rich medium. the culture conditions, including sgf concentration, agitation speed, initial ph, temperature, and time, were optimized as follows: 7% (w/v) sgf, initial ph 6.0, 33 °c, 300 rpm, and 24 h in a 100 ml baffled flask. the determined amount of isoflavone aglycons in sgf using 50% ethyl acetate was the ... | 2013 | 24266868 |
| conjugation of deoxynivalenol by alternaria alternata (54028 nrrl), rhizopus microsporus var. rhizopodiformis (54029 nrrl) and aspergillus oryzae (5509 nrrl). | deoxynivalenol (don, vomitoxin) is a trichothecene mycotoxin which can be considered to be an indicator of fusarium mycotoxin contamination in grain, feed and food. recent studies have described the presence of glucose conjugated don, which is a product of plant metabolism, but there is a lack of information available on don conjugation by fungi. the aim of the current study was, therefore, to investigate the ability of fungi to metabolize don into hydrolysable conjugated don. alternaria alterna ... | 2014 | 24263850 |
| enzymatic synthesis of novel oligosaccharides from n-acetylsucrosamine using β-fructofuranosidase from aspergillus oryzae. | mycelia of aspergillus oryzae nbrc100959 contain 2 types of β-fructofuranosidases, transfructosylation-catalyzing enzyme (βffasei), and hydrolysis-catalyzing enzyme (βffaseii). using βffasei extracted from the mycelia of strain nbrc100959, two novel oligosaccharides consisting of glcnac and fructose, β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl-(2↔1)-2-acetamido-2-deoxy-α-d-glucopyranoside (n-acetyl-1-kestosamine, 1-kesnac) and β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl-(2→1)-β-d-fructofuran ... | 2013 | 24246554 |
| purification and characterization of a prolyl endopeptidase isolated from aspergillus oryzae. | a new fungal strain that was isolated from our library was identified as an aspergillus oryzae and noted to produce a novel proly endopeptidase. the enzyme was isolated, purified, and characterized. the molecular mass of the prolyl endopeptidase was estimated to be 60 kda by using sds-page. further biochemical characterization assays revealed that the enzyme attained optimal activity at ph 4.0 with acid ph stability from 3.0 to 5.0. its optimum temperature was 30 °c and residual activity after 3 ... | 2014 | 24241934 |
| molecular identification of isolated fungi from stored apples in riyadh, saudi arabia. | fungi causes most plant disease. when fruits are stored at suboptimal conditions, fungi grows, and some produce mycotoxin which can be dangerous for human consumption. studies have shown that the penicillium and monilinia species commonly cause spoilage of fruits, especially apples. several other genera and species were reported to grow to spoil fruits. this study was conducted to isolate and identify fruit spoilage by fungi on apples collected in riyadh, saudi arabia and conduct a molecular ide ... | 2013 | 24235866 |
| oxygen fluorescence quenching studies with single tryptophan-containing proteins. | the work of lakowicz and weber [biochemistry 12, 4161 (1973)] demonstrated that molecular oxygen is a powerful quencher of tryptophan fluorescence in proteins. here we report studies of the oxygen quenching of several proteins that have a single, internal tryptophan residue. among these are apoazurin (pseudomonas aeruginosa), asparaginase (escherichia coli), ribonuclease t1 (aspergillus oryzae), and cod parvalbumin. both fluorescence intensity and phase lifetime quenching data are reported. by c ... | 1994 | 24233381 |
| effects of direct-fed microbial supplementation on broiler performance, intestinal nutrient transport and integrity under experimental conditions with increased microbial challenge. | 1. the effects of aspergillus oryzae- and bacillus subtilis-based direct-fed microbials (dfm) were investigated on the performance, ileal nutrient transport and intestinal integrity of broiler chickens, raised under experimental conditions, with increased intestinal microbial challenge. 2. the first study was a 3 × 2 factorial experiment, with 3 dietary treatments (control (con), con + dfm and con + antibiotic growth promoter) with and without challenge. chicks were fed experimental diets from 1 ... | 2014 | 24219515 |
| improved α-amylase production by aspergillus oryzae after a double deletion of genes involved in carbon catabolite repression. | in filamentous fungi, the expression of secretory glycoside hydrolase encoding genes, such as those for amylases, cellulases, and xylanases, is generally repressed in the presence of glucose. crea and creb have been observed to be regulating factors for carbon catabolite repression. in this study, we generated single and double deletion crea and/or creb mutants in aspergillus oryzae. the α-amylase activities of each strain were compared under various culture conditions. for the wild-type strain, ... | 2014 | 24213479 |
| efficient formation of heterokaryotic sclerotia in the filamentous fungus aspergillus oryzae. | heterokaryon formation by hyphal fusion occurs during a sexual/parasexual cycle in filamentous fungi, and therefore, it is biotechnologically important for crossbreeding. in the industrial filamentous fungus aspergillus oryzae, a parasexual cycle has been reported, and it was recently suggested that sexual reproduction should be possible. however, as a. oryzae enters into hyphal fusion with a much lower frequency than neurospora crassa, the process of heterokaryon formation has not been extensiv ... | 2014 | 24201891 |
| production and characterization of a new α-glucosidase inhibitory peptide from aspergillus oryzae n159-1. | an α-glucosidase inhibitor was developed from aspergillus oryzae n159-1, which was screened from traditional fermented korean foods. the intracellular concentration of the inhibitor reached its highest level when the fungus was cultured in tryptic soy broth medium at 27℃ for five days. the inhibitor was purified using a series of purification steps involving ultrafiltration, sephadex g-25 gel permeation chromatography, strong cation exchange solid phase extraction, reverse-phase high performance ... | 2013 | 24198670 |
| the aspergillus genome database: multispecies curation and incorporation of rna-seq data to improve structural gene annotations. | the aspergillus genome database (aspgd; http://www.aspgd.org) is a freely available web-based resource that was designed for aspergillus researchers and is also a valuable source of information for the entire fungal research community. in addition to being a repository and central point of access to genome, transcriptome and polymorphism data, aspgd hosts a comprehensive comparative genomics toolbox that facilitates the exploration of precomputed orthologs among the 20 currently available asperg ... | 2014 | 24194595 |
| identification of pyroglutamyl peptides in japanese rice wine (sake): presence of hepatoprotective pyroglu-leu. | japanese rice wine, sake, is made from steamed rice, water, and lactic acid by "multiple parallel fermentation" with mold (aspergillus oryzae) and yeast (saccharomyces cerevisiae). nineteen pyroglutamyl peptides were identified in commercially available sake. among them, pyroglu-leu and pyroglu-gln were the major constituents. pyroglu-leu has been demonstrated to attenuate hepatitis and colitis in animal models. commercial products (n = 5) contained pyroglu-leu at concentrations ranging from 40 ... | 2013 | 24175632 |
| improvement of kojic acid production in aspergillus oryzae b008 mutant strain and its uses in fermentation of concentrated corn stalk hydrolysate. | a strain designated m866, producing kojic acid with a high yield, was obtained by combining induced mutation using ion beam implantation and ethyl methane sulfonate treatment of a wild type strain of aspergillus oryzae b008. the amount of kojic acid produced by the strain m866 in a shaking flask was 40.2 g/l from 100 g/l of glucose, which was 1.7 times higher than that produced by wild strain (23.58 g/l). when the mixture of glucose and xylose was used as carbon source, the resulting kojic acid ... | 2014 | 24170020 |
| fed-batch synthesis of galacto-oligosaccharides with aspergillus oryzae β-galactosidase using optimal control strategy. | fed-batch synthesis of galacto-oligosaccharides (gos) from lactose with β-galactosidase from aspergillus oryzae was evaluated experimentally and reaction yield was maximized via optimal control technique. the optimal lactose and enzyme feed flow rate profiles were determined using a model for gos synthesis previously reported by the authors. experimentally it was found that fed-batch synthesis allowed an increase on the maximum total gos concentration from 115 (batch synthesis) to 218 g l(-1) as ... | 2014 | 24167086 |
| purification and partial characterization of nad aminohydrolase from aspergillus oryzae nrrl447. | aspergillus oryzae aminohydrolase free acid phosphodiesterase catalyzes nicotinamide adenine dinucleotide to deamino-nad and ammonia. the enzyme was purified to homogeneity by a combination of acetone precipitation, anion exchange chromatography and gel filtration chromatography. the enzyme was purified 230.5 fold. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme showed a single protein band of mw 94 kda. the enzyme displayed maximum activity at ph 5 and 40 °c wit ... | 2014 | 24158390 |
| galactooligosaccharides formation during enzymatic hydrolysis of lactose: towards a prebiotic-enriched milk. | the formation of galactooligosaccharides (gos) in skim milk during treatment with several commercial β-galactosidases (bacillus circulans, kluyveromyces lactis and aspergillus oryzae) was analysed in detail, at 4 and 40°c. the maximum gos concentration was obtained at a lactose conversion of approximately 40-50% with b. circulans and a. oryzae β-galactosidases, and at 95% lactose depletion for k. lactis β-galactosidase. using an enzyme dosage of 0.1% (v/v), the maximum gos concentration with k. ... | 2014 | 24128493 |
| kinetic characterization of galacto-oligosaccharide (gos) synthesis by three commercially important β-galactosidases. | many β-galactosidases show large differences in galacto-oligosaccharide (gos) production and lactose hydrolysis. in this study, a kinetic model is developed in which the effect of lactose, glucose, galactose, and oligosaccharides on the onpg converting activity of various β-galactosidases is quantified. the use of onpg as a competing substrate to lactose yields more information than can be obtained by examining only the conversion of lactose itself. the reaction rate with lactose or oligosacchar ... | 2014 | 24124064 |
| a new diketopiperazine alkaloid from aspergillus oryzae. | investigation of bioactive secondary metabolites from terrestrial aspergillus oryzae sp. mmao1 using m2 medium afforded a new diketopiperazine alkaloid, 7,9-dihydroxy-3-(1h-indol-3-ylmethyl)-8-methoxy-2,3,11,11a-tetrahydro-6h-pyrazino[1,2-b]isoquinoline-1,4-dione (1a), containing the unusual amino acid l-6,8-dihydroxy-7-methoxyphenylalanine. this was co-isolated with ditryptophenaline (2), cyclo-(tryp,tyr) (4), cyclo-(pro,val), α-cyclopiazonic acid (3), kojic acid and uridine. re-cultivation of ... | 2014 | 24116376 |
| glycosylation analysis of recombinant neutral protease i from aspergillus oryzae expressed in pichia pastoris. | neutral protease i from aspergillus oryzae 3.042 was expressed in pichia pastoris and its n-glycosylation properties were analyzed. after purification by nickel-affinity chromatography column, the recombinant neutral protease (rnpi) was confirmed to be n-glycosylated by periodicacid/schiff's base staining and endo h digestion. moreover, the deglycosylated protein's molecular weight decreased to 43.3 kda from 54.5 kda analyzed by sds-page and maldi-tof-ms, and the hyperglycosylation extent was 21 ... | 2013 | 24078118 |
| drosera peltata smith var. lunata (buch.-ham.) c. b. clarke as a feasible source of plumbagin: phytochemical analysis and antifungal activity assay. | drosera peltata smith var. lunata (buch.-ham.) c. b. clarke (dpvl) fractions and plumbagin were tested via broth microdilution techniques on rhizopus oryzae, aspergillus flavus, aspergillus niger, aspergillus oryzae, penicillium citrinum. all of the test substances [petroleum ether, chloroform, ethyl acetate, n-butanol fraction and aqueous residue (ar)] except for the ar were active against all the tested strains. the petroleum ether fraction (pef) was the most active (mic = 5.86-46.88 μg/ml, mf ... | 2014 | 24078108 |
| fungal spore concentrations in indoor and outdoor air in university libraries, and their variations in response to changes in meteorological variables. | the fungal spore concentration (fsc) in the air poses a risk for human health. this work studied the fsc in university libraries and how it is affected by environmental factors. a total of 347 samples were obtained using a microbio mb2(®) aerosol sampler. the wind speed (ws), cross wind (cw), temperature (t), relative humidity (hr), barometric pressure (bp) and dew point (dp) were recorded using a kestrel(®) 4500 weather station. the median indoor/outdoor fsc was 360/1230 cfu m(-3). fsc correlat ... | 2014 | 24070332 |
| effect of the combined physical and chemical treatments with microbial fermentation on corn straw degradation. | in order to improve corn straw degradation, steam explosion, sodium hydroxide soaking and aspergillus oryzae fermentation were used. the optimal sodium hydroxide pretreatment condition for lignin degradation was obtained. the degradation rates of hemicellulose, cellulose and lignin were 54.68%, 17.76% and 33.14% for the exploded straw (p<0.05); 67.92%, 2.44% (p>0.05) and 76.54% for the alkali-treated straw (p<0.05); 75.98%, 39.93% and 77.88% for the exploded and alkali-treated straw (p<0.05), re ... | 2013 | 24063818 |
| the crystal structure of an extracellular catechol oxidase from the ascomycete fungus aspergillus oryzae. | catechol oxidases (ec 1.10.3.1) catalyse the oxidation of o-diphenols to their corresponding o-quinones. these oxidases contain two copper ions (cua and cub) within the so-called coupled type 3 copper site as found in tyrosinases (ec 1.14.18.1) and haemocyanins. the crystal structures of a limited number of bacterial and fungal tyrosinases and plant catechol oxidases have been solved. in this study, we present the first crystal structure of a fungal catechol oxidase from aspergillus oryzae (aoco ... | 2013 | 24043469 |
| recombinant aspergillus β-galactosidases as a robust glycomic and biotechnological tool. | galactosidases are widespread enzymes that are used for manifold applications, including production of prebiotics, biosynthesis of different transgalactosylated products, improving lactose tolerance and in various analytical approaches. the nature of these applications often require galactosidases to be present in a purified form with clearly defined properties, including precisely determined substrate specificities, low sensitivity to inhibitors, and high efficiency and stability under distinct ... | 2014 | 24037406 |
| the hydrolysis of agro-industrial residues by holocellulose-degrading enzymes. | holocellulose structures from agro-industrial residues rely on main and side chain attacking enzymes with different specificities for complete hydrolysis. combinations of crude enzymatic extracts from different fungal species, including aspergillus terreus, aspergillus oryzae, aspergillus niger and trichoderma longibrachiatum, were applied to sugar cane bagasse, banana stem and dirty cotton residue to investigate the hydrolysis of holocellulose structures. a. terreus and a. oryzae were the best ... | 2012 | 24031857 |
| kinetic analysis of batch ethanol acetylation in isothermal non-stationary multiphase systems by lyophilized mycelium of aspergillus oryzae. | a relatively complex network of reactions has been investigated, using as a network model the isothermal batch esterification of acetic acid with ethanol in n-heptane catalyzed by lyophilized mycelium of aspergillus oryzae. the kinetic analysis was firstly carried out on the whole system, without any simplification, by means of the well-known integral method. owing to the poor results obtained by this way, we developed an alternative approach, combining initial rates and integral analysis and re ... | 2011 | 24031645 |
| mutagenesis of aspergillus oryzae ipt-301 to improve the production of β-fructofuranosidase. | aspergillus oryzae ipt-301, previously reported as a β-fructofuranosidase producing microorganism, was successfully mutated using uv irradiation at 253.7 nm followed by the screening of survivors resistant to certain stress conditions. strains were first subjected to the β-fructofuranosidase activity assay using a portion from the colony grown in petri dish as the enzyme source. seven mutants with β-fructofuranosidase activity values relative to the parent culture between 140 - 190% were selecte ... | 2010 | 24031480 |
| effects of food lectins on the transport system of human intestinal caco-2 cell monolayers. | the effects of 16 lectins isolated from foodstuff on the transport system across human intestinal caco-2 cell monolayers were investigated by using four fluorescent markers: lucifer yellow (ly) for the paracellular pathway, fluorescein (fl) for the monocarboxylic acid transporter-mediated pathway, rhodamine 123 for the p-glycoprotein-mediated efflux pathway, and calcein for the multidrug resistance associated protein-related efflux pathway. the transepithelial electrical resistance (ter) values ... | 2013 | 24018688 |
| aspergillus oryzae type iii polyketide synthase csyb uses a fatty acyl starter for the biosynthesis of csypyrone b compounds. | csypyrones b1, b2 and b3 are α-pyrones that can be obtained from aspergillus oryzae expressing csyb, which is a type iii polyketide synthase. we investigated the biosynthesis of the csypyrone b compounds using [1-(13)c] and [2-(13)c] acetate feeding experiments. (13)c nmr analyses of the methyl esters of the csypyrone b compounds fed with the (13)c-labeled acetates showed that the carboxyl carbons of the csypyrone b side-chains were derived from the c-2 methyl carbon of the acetate. these result ... | 2013 | 24011646 |
| microbial floral dynamics of chinese traditional soybean paste (doujiang) and commercial soybean paste. | traditional soybean paste from shandong liangshan and tianyuan jiangyuan commercial soybean paste were chosen for analysis and comparison of their bacterial and fungal dynamics using denaturing gel gradient electrophoresis and 16s rrna gene clone libraries. the bacterial diversity results showed that more than 20 types of bacteria were present in traditional shandong soybean paste during its fermentation process, whereas only six types of bacteria were present in the commercial soybean paste. th ... | 2013 | 24002452 |
| screening of extracts from natural feed ingredients for their ability to reduce enterotoxigenic escherichia coli (etec) k88 adhesion to porcine intestinal epithelial cell-line ipec-j2. | enterotoxigenic escherichia coli (etec) k88 is the most prevalent enteropathogen in weaned piglets, with the ability to express fimbria f4 and specifically attach to intestinal receptors in the young piglet. the prevention of etec k88 adhesion to the epithelium by interfering in this fimbria-receptor recognition provides an alternative approach to prevent the initial stage of disease. the aim of this study is to screen, among different feed ingredients (fi), their ability to reduce etec k88 atta ... | 2013 | 23992796 |
| aspergillus oryzae aoso is a novel component of stress granules upon heat stress in filamentous fungi. | stress granules are a type of cytoplasmic messenger ribonucleoprotein (mrnp) granule formed in response to the inhibition of translation initiation, which typically occurs when cells are exposed to stress. stress granules are conserved in eukaryotes; however, in filamentous fungi, including aspergillus oryzae, stress granules have not yet been defined. for this reason, here we investigated the formation and localization of stress granules in a. oryzae cells exposed to various stresses using an e ... | 2013 | 23991062 |
| random mutagenesis of the aspergillus oryzae genome results in fungal antibacterial activity. | multidrug-resistant bacteria cause severe infections in hospitals and communities. development of new drugs to combat resistant microorganisms is needed. natural products of microbial origin are the source of most currently available antibiotics. we hypothesized that random mutagenesis of aspergillus oryzae would result in secretion of antibacterial compounds. to address this hypothesis, we developed a screen to identify individual a. oryzae mutants that inhibit the growth of methicillin-resista ... | 2013 | 23983696 |
| purification and characterization of intracellular cellulase from aspergillus oryzae itcc-4857.01. | purification and characterization of intracellular cellulase produced by a. oryzae itcc-4857.01 are reported. the enzyme was purified by ion-exchange chromatography using deae-cellulose followed by gel filtration. the purification achieved was 41 fold from the crude extract with yield of 27%. the purified enzyme showed single band on poly acrylamide gel. the molecular weight as determined by sds-page and gel filtration was 38 kda and 38.6 kda respectively and contained only one subunit. the enzy ... | 2009 | 23983520 |
| reduction of the degradation activity of umami-enhancing purinic ribonucleotide supplement in miso by the targeted suppression of acid phosphatases in the aspergillus oryzae starter culture. | miso (fermented soybean paste) is a traditional japanese fermented food, and is now used worldwide. the solid-state culture of filamentous fungus, aspergillus oryzae, grown on rice is known as rice-koji, and is important as a starter for miso fermentation because of its prominent hydrolytic enzyme activities. recently, commercial miso products have been supplemented with purinic ribonucleotides, such as inosine monophosphate (imp) and guanine monophosphate, to enhance the characteristic umami ta ... | 2013 | 23973834 |
| kinetics of improved 1,4-alpha-d-glucan glucohydrolase biosynthesis from a newly isolated aspergillus oryzae iib-6 and parameter significance analysis by 2-factorial design. | sixteen different mould cultures viz. aspergillus, alternaria, arthroderma, trichoderma, fusarium, penicillium, rhizopus and chochliobolus were isolated from the soil samples of qatar by serial dilution method. the preliminary screening of isolates was done by selecting initial colonies showing relatively bigger zones of starch hydrolysis on nutrient agar plates. the isolates were then subjected to secondary screening by submerged fermentation (smf). the 1,4-α-d-glucan glucohydrolase (ggh) activ ... | 2012 | 23961361 |
| bismuth-inhibitory effects on bacteria and stimulation of fungal growth in vitro. | bismuth salicylate was found to inhibit the growth of a range of bacteria and yeast, "candida albicans". in general the growth of bacteria did not result in increase in bismuth solubilisation, in contrast, bismuth solubilisation increased following the growth of c. albicans. a significant increase in the biomass (dry weight) of aspergillus niger and aspergillus oryzae occurred in vitro when these fungi were grown in the presence of bismuth salicylate. biomass increase occurred over a range of bi ... | 2012 | 23961173 |
| protease inhibitor from moringa oleifera with potential for use as therapeutic drug and as seafood preservative. | protease inhibitors are well known to have several applications in medicine and biotechnology. several plant sources are known to return potential protease inhibitors. in this study plants belonging to different families of leguminosae, malvaceae, rutaceae, graminae and moringaceae were screened for the protease inhibitor. among them moringa oleifera, belonging to the family moringaceae, recorded high level of protease inhibitor activity after ammonium sulfate fractionation. m. oleifera, which g ... | 2011 | 23961135 |
| regulation of development in aspergillus nidulans and aspergillus fumigatus. | members of the genus aspergillus are the most common fungi and all reproduce asexually by forming long chains of conidiospores (or conidia). the impact of various aspergillus species on humans ranges from beneficial to harmful. for example, several species including aspergillus oryzae and aspergillus niger are used in industry for enzyme production and food processing. in contrast, aspergillus flavus produce the most potent naturally present carcinogen aflatoxins, which contaminate various plant ... | 2010 | 23956662 |
| metabolic engineering of aspergillus oryzae nrrl 3488 for increased production of l-malic acid. | malic acid, a petroleum-derived c4-dicarboxylic acid that is used in the food and beverage industries, is also produced by a number of microorganisms that follow a variety of metabolic routes. several members of the genus aspergillus utilize a two-step cytosolic pathway from pyruvate to malate known as the reductive tricarboxylic acid (rtca) pathway. this simple and efficient pathway has a maximum theoretical yield of 2 mol malate/mol glucose when the starting pyruvate originates from glycolysis ... | 2013 | 23925533 |
| engineering the thermostability of a xylanase from aspergillus oryzae by an enhancement of the interactions between the n-terminus extension and the β-sheet a2 of the enzyme. | a mesophilic aspergillus oryzae xylanase (aoxyn11a) belongs to glycoside hydrolase family 11. hydrogen bonds and a disulfide bridge were introduced between the n-terminus extension and the β-sheet a2 of aoxyn11a, which were located in the corresponding region of a hyperthermostable xylanase. the mutants were designated as aoxyn11a(c5) and aoxyn11a(c5-c32), respectively. the thermostabilities of aoxyn11a and the mutants were assessed by the molecular dynamics simulations. after being incubated at ... | 2013 | 23907668 |
| investigation of malic acid production in aspergillus oryzae under nitrogen starvation conditions. | malic acid has great potential for replacing petrochemical building blocks in the future. for this application, high yields, rates, and titers are essential in order to sustain a viable biotechnological production process. natural high-capacity malic acid producers like the malic acid producer aspergillus flavus have so far been disqualified because of special growth requirements or the production of mycotoxins. as a. oryzae is a very close relative or even an ecotype of a. flavus, it is likely ... | 2013 | 23892740 |
| comparison and analysis of the genomes of two aspergillus oryzae strains. | a. oryzae 3.042 (china) and a. oryzae rib40 (japan) used for soy sauce fermentation show some regional differences. we sequenced the genome of a. oryzae 3.042 and compared it to a. oryzae rib40 in an attempt to understand why different features are shown by these two a. oryzae strains. we predict 11,399 protein-coding genes in a. oryzae 3.042. the genomes of these two a. oryzae strains are collinear revealed by mummer analysis, indicating that the differences are not obvious between them. severa ... | 2013 | 23889147 |
| mycoflora of soybeans used for meju fermentation. | diverse fungi are present in korean traditional meju and they are known to play an important role in fermented soybean products. to determine the origin of the fungi in meju, we examined the mycoflora of soybeans from 10 traditional meju factories. the samples were untreated or treated with sodium hypochlorite, and placed on malt extract agar (mea), dichloran 18% glycerol agar (dg18), and dichloran rose bengal chloramphenicol agar (drbc) medium. a total of 794 fungal strains were isolated and th ... | 2013 | 23874133 |
| assessment of a bacterial 6-phytase in the diets of broiler chickens. | two 21-d broiler experiments were conducted to assess the efficacy of a bacterial 6-phytase expressed in aspergillus oryzae on growth performance, nutrient utilization, and intestinal molecular markers. two hundred forty birds in 5 treatments (experiment 1) or 256 birds in 4 treatments (experiment 2) were used. the treatments included a negative control diet that was marginally deficient in p (nc) or nc plus tricalcium phosphate, 500, 1,000, or 2,000 phytase units/kg (experiment 1), and nc or nc ... | 2013 | 23873558 |
| deletion of creb in aspergillus oryzae increases secreted hydrolytic enzyme activity. | aspergillus oryzae has been used in the food and beverage industry for centuries, and industrial strains have been produced by multiple rounds of selection. targeted gene deletion technology is particularly useful for strain improvement in such strains, particularly when they do not have a well-characterized meiotic cycle. phenotypes of an aspergillus nidulans strain null for the creb deubiquitinating enzyme include effects on growth and repression, including increased activity levels of various ... | 2013 | 23835170 |
| effect of the combined probiotics with aflatoxin b₁-degrading enzyme on aflatoxin detoxification, broiler production performance and hepatic enzyme gene expression. | in order to degrade aflatoxin b₁ (afb₁), afb₁-degrading microbes (probiotics) such as lactobacillus casei, bacillus subtilis and pichia anomala, and the afb₁-degrading enzyme from aspergillus oryzae were selected and combined to make feed additive. seventy-five 43-day-old male arbor acres broilers were randomly divided into 5 groups, 15 broilers for each group. the broilers were given with 5 kinds of diets such as the basal diet, 400 μg/kg afb₁ supplement without feed additive, and 200, 400, 800 ... | 2013 | 23831311 |
| characterization of a (d)-stereoselective aminopeptidase (dama) exhibiting aminolytic activity and halophilicity from aspergillus oryzae. | β-aminopeptidases exhibit both hydrolytic and aminolytic (peptide bond formation) activities and have only been reported in bacteria. we identified a gene encoding the β-aminopeptidase homolog from a genome database of the filamentous fungus aspergillus oryzae. the gene was overexpressed in a. oryzae, and the resulting recombinant enzyme was purified. apart from bacterial homologs [β-ala-para-nitroanilide (pna)], the enzyme preferred d-leu-pna and d-phe-pna as substrates. therefore, we designate ... | 2013 | 23821291 |
| separation and characterization of the immunostimulatory components in unpolished rice black vinegar (kurozu). | unpolished rice black vinegar (kurozu), a traditional japanese vinegar, is considered to have beneficial health effects. kurozu is produced via a static fermentation process involving the saccharification of rice by aspergillus oryzae, alcohol fermentation by saccharomyces cerevisiae, and the oxidation of ethanol to acetic acid by acetic acid bacteria such as acetobacter pasteurianus. since this process requires about 6 months' fermentation and then over a year of aging, most of these organisms ... | 2013 | 23810669 |
| synthesis and antifungal activities of cyclic octa-lipopeptide burkholdine analogues. | synthesis and antifungal activity of cyclic octapeptide derivatives of burkholdines are described. to construct cyclic octapeptides, the combination of fmoc-spps and cyclization with dic/hobt in the solution phase was employed. synthesized peptides were evaluated for antifungal activity with mic values against saccharomyces cerevisiae, aspergillus oryzae, and candida viswanathii. as a result, the lipid side chain and the stereochemistry of each amino acid of bk-1097 analogues significantly affec ... | 2013 | 23769641 |
| structurally diverse chaetophenol productions induced by chemically mediated epigenetic manipulation of fungal gene expression. | epigenetic manipulation of gene expression in chaetomium indicum using a hdac inhibitor led to the isolation of structurally diverse chaetophenols, and 3, 4 and 5 bear unprecedented polycyclic skeletons. the expression of two silent genes (pksch-1 and pksch-2) for nonreducing pkss involved in chaetophenol biosynthesis was associated with an increase of histone acetylation level. the heterologous gene expression study in aspergillus oryzae revealed pksch-2 to be the nr-pks gene for 8. | 2013 | 23767797 |
| crystal structures of copper-depleted and copper-bound fungal pro-tyrosinase: insights into endogenous cysteine-dependent copper incorporation. | tyrosinase, a dinuclear copper monooxygenase/oxidase, plays a crucial role in the melanin pigment biosynthesis. the structure and functions of tyrosinase have so far been studied extensively, but the post-translational maturation process from the pro-form to the active form has been less explored. in this study, we provide the crystal structures of aspergillus oryzae full-length pro-tyrosinase in the holo- and the apo-forms at 1.39 and 2.05 å resolution, respectively, revealing that phe(513) on ... | 2013 | 23749993 |
| production of 8-hydroxydaidzein from soybean extract by aspergillus oryzae kacc 40247. | aspergillus oryzae kacc 40247 was selected from among 60 fungal strains as an effective 7,8,4'-trihydroxyisoflavone (8-hydroxydaidzein)-producing fungus. the optimal culture conditions for production by this strain in a 7-l fermentor were found to be 30 °c, ph 6, and 300 rpm. under these conditions, a. oryzae kacc 40247 produced 62 mg/l of 8-hydroxydaidzein from soybean extract in 30 h, with a productivity of 2.1 mg/l/h. these are the highest production and productivity for 8-hydroxydaidzein eve ... | 2013 | 23748754 |
| use of spent osmotic solutions for the production of fructooligosaccharides by aspergillus oryzae n74. | in the food industry, osmotic dehydration can be an important stage to obtain partially dry foodstuffs. however, the remaining spent osmotic solution at the end of the process could become a waste with an important environmental impact due to the large amount of organic compounds that it might contain. since one of the most important osmotic agents used in osmotic dehydration is sucrose, this spent osmotic solution could be used to be biotransformed to produce fructooligosaccharides by a fructos ... | 2014 | 23744119 |
| fungus-specific sirtuin hstd coordinates secondary metabolism and development through control of laea. | the sirtuins are members of the nad(+)-dependent histone deacetylase family that contribute to various cellular functions that affect aging, disease, and cancer development in metazoans. however, the physiological roles of the fungus-specific sirtuin family are still poorly understood. here, we determined a novel function of the fungus-specific sirtuin hstd/aspergillus oryzae hst4 (aohst4), which is a homolog of hst4 in a. oryzae yeast. the deletion of all histone deacetylases in a. oryzae demon ... | 2013 | 23729383 |
| rapid enzyme production and mycelial growth in solid-state fermentation using the non-airflow box. | solid-state fermentation (ssf) has become an attractive alternative to submerged fermentation (smf) for the production of enzymes, organic acids, and secondary metabolites, while there are many problems during the culture of ssf. we recently created a ssf system using a non-airflow box (nab) in order to resolve the problems, which enabled the uniform culture in the whole substrate and high yield of many enzymes. in this paper, further characterization of ssf using the nab was carried out to obta ... | 2013 | 23727349 |
| a compendium of causative agents of occupational asthma. | the objective is to provide an evidence-based compendium of allergenic and irritant agents that are known to cause occupational asthma in order to improve diagnostics and disease management. | 2013 | 23706060 |
| [influence of fungal elicitor and macroporous resin on shikonin accumulation in hairy roots of arnebia euchroma (royle) johnst]. | in order to investigate the effects of fungal elicitor and macroporous adsorption resin on shikonin accumulation in hairy roots of arnebia euchroma (royle) johnst, we used spectrophotometry to determine the total naphthoquinone content of the hairy roots, by adding different volume ratio of aspergillus niger elicitor, aspergillus oryzae elicitor, and the macroporous resin into the m-9 liquid medium at different culture time. the results show that the total naphthoquinone content was 2.28 times h ... | 2013 | 23697166 |
| molecular markers for barrett's esophagus and its progression to cancer. | there is a clinical need for biomarkers that can improve diagnostic accuracy and risk stratification of esophageal lesions. here we review the current literature and highlight the most important, recent advancements in biomarkers as a supplement to histopathology for management of patients with barrett's esophagus. | 2013 | 23689523 |
| the crystal structure of acidic β-galactosidase from aspergillus oryzae. | the crystal structure of the industrially important aspergillus oryzae β-galactosidase has been determined at 2.60 å resolution. the ao-β-gal is a large (985 residues) monomeric multi-domain enzyme that has a catalytic (α/β)8-barrel domain. an electron density map revealed extensive n-glycosylation between the domain interfaces suggesting that the oligosaccharide-chains would have a stabilizing role for the structure of ao-β-gal. comparison of structure with other β-galactosidase structures of g ... | 2013 | 23688418 |
| comparative genomic analysis of aspergillus oryzae strains 3.042 and rib40 for soy sauce fermentation. | the filamentous fungus aspergillus oryzae 3.042 (chinese strain) is a close relative of a. oryzae rib40 (japanese strain), which is the important agent used for soy sauce fermentation. the genome of a. oryzae 3.042 was sequenced and compared with a. oryzae rib40 in an attempt to understand why different soy sauce flavors are produced by these strains. the a. oryzae 3.042 chromosome is 36,547,279bp and contains 11,399 protein-encoding genes. mummer analysis revealed that the genomes of a. oryzae ... | 2013 | 23673060 |
| gamma radiation mediated green synthesis of gold nanoparticles using fermented soybean-garlic aqueous extract and their antimicrobial activity. | aspergillus oryzae was used to enhance the mobilization of antioxidants of soybean matrix along with garlic as a co-substrate by modulating polyphenolic substances during solid-state fermentation. mobilized polyphenols were used as a green tool for synthesis and stabilization of gold nanoparticles (aunps). the radiation-induced aunps synthesis is a simple, clean and inexpensive process which involves radiolysis of aqueous solution that provides an efficient method to reduce metal ions. gamma irr ... | 2013 | 23667801 |
| fine de novo sequencing of a fungal genome using only solid short read data: verification on aspergillus oryzae rib40. | the development of next-generation sequencing (ngs) technologies has dramatically increased the throughput, speed, and efficiency of genome sequencing. the short read data generated from ngs platforms, such as solid and illumina, are quite useful for mapping analysis. however, the solid read data with lengths of <60 bp have been considered to be too short for de novo genome sequencing. here, to investigate whether de novo sequencing of fungal genomes is possible using only solid short read seque ... | 2013 | 23667655 |
| base composition and nucleosome density in exonic and intronic regions in genes of the filamentous ascomycetes aspergillus nidulans and aspergillus oryzae. | we sequenced nucleosomal dna fragments of the filamentous ascomycetes aspergillus nidulans and aspergillus oryzae and then mapped those sequences on their genomes. we compared the gc content and nucleosome density in the exonic and intronic regions in the genes of a. nidulans and a. oryzae. although the gc content and nucleosome density in the exonic regions tended to be higher than those in the intronic regions, the difference in the distribution of the gc content was more notable than that of ... | 2013 | 23664982 |
| enhanced production of bovine chymosin by autophagy deficiency in the filamentous fungus aspergillus oryzae. | aspergillus oryzae has been utilized as a host for heterologous protein production because of its high protein secretory capacity and food-safety properties. however, a. oryzae often produces lower-than-expected yields of target heterologous proteins due to various underlying mechanisms, including degradation processes such as autophagy, which may be a significant bottleneck for protein production. in the present study, we examined the production of heterologous protein in several autophagy (aoa ... | 2013 | 23658635 |
| improved production of protease-resistant phytase by aspergillus oryzae and its applicability in the hydrolysis of insoluble phytates. | among three hundred isolates of filamentous fungi, aspergillus oryzae sbs50 secreted higher phytase activity at ph 5.0, 35 °c and 200 rpm after 96 h of fermentation. starch and beef extract supported the highest phytase production than other carbon and nitrogen sources. a nine-fold improvement in phytase production was achieved due to optimization. supplementation of the medium with inorganic phosphate repressed the enzyme synthesis. among surfactants tested, tween 80 increased fungal growth and ... | 2013 | 23652971 |
| antifungal effect of allium tuberosum, cinnamomum cassia, and pogostemon cablin essential oils and their components against population of aspergillus species. | antifungal activity of allium tuberosum (at), cinnamomum cassia (cc), and pogostemon cablin (patchouli, p) essential oils against aspergillus flavus strains 3.2758 and 3.4408 and aspergillus oryzae was tested at 2 water activity levels (aw : 0.95 and 0.98). main components of tested essential oils were: allyl trisulfide 40.05% (at), cinnamaldehyde 87.23% (cc), and patchouli alcohol 44.52% (p). the minimal inhibitory concentration of the plant essential oils against a. flavus strains 3.2758 and 3 ... | 2013 | 23647469 |
| high-efficiency removal of phytic acid in soy meal using two-stage temperature-induced aspergillus oryzae solid-state fermentation. | phytic acid of soy meal (sm) could influence protein and important mineral digestion of monogastric animals. aspergillus oryzae (atcc 9362) solid-state fermentation was applied to degrade phytic acid in sm. two-stage temperature fermentation protocol was investigated to increase the degradation rate. the first stage was to maximize phytase production and the second stage was to realize the maximum enzymatic degradation. | 2014 | 23633040 |
| a robust whole-cell biocatalyst that introduces a thermo- and solvent-tolerant lipase into aspergillus oryzae cells: characterization and application to enzymatic biodiesel production. | to develop a robust whole-cell biocatalyst that works well at moderately high temperature (40-50°c) with organic solvents, a thermostable lipase from geobacillus thermocatenulatus (btl2) was introduced into an aspergillus oryzae whole-cell biocatalyst. the lipase-hydrolytic activity of the immobilized a. oryzae (r-btl) was highest at 50°c and was maintained even after an incubation of 24-h at 60°c. in addition, r-btl was highly tolerant to 30% (v/v) organic solvents (dimethyl carbonate, ethanol, ... | 2013 | 23608501 |
| biodegradation of nicotine by a novel nicotine-degrading bacterium, pseudomonas plecoglossicida tnd35 and its new biotransformation intermediates. | tobacco wastes that contain nicotine alkaloids are harmful to human health and the environment. in the investigation, a novel nicotine-biodegrading bacterium tnd35 was isolated and identified as pseudomonas plecoglossicida on the basis of phenotypic, biochemical characteristics and 16s rrna sequence homology. we have studied the nicotine biodegradation potential of strain tnd35 by detecting the intermediate metabolites using an array of approaches such as hplc, gc-ms, nmr and ft-ir. biotransform ... | 2014 | 23604517 |
| microbial lipid production from potato processing wastewater using oleaginous filamentous fungi aspergillus oryzae. | use of potato processing wastewater for microbial lipid production by oleaginous filamentous fungus aspergillus oryzae was studied with the purpose of recycling potato processing wastewater for biodiesel production. the wastewater contained high concentrations of solids, starch and nutrients. sterilization of the potato processing wastewater resulted in a thick gelatinized medium, causing the fungi to grow slow. in order to overcome this problem, the wastewater was diluted with tap water at thre ... | 2013 | 23597680 |
| functional analysis of abp1p-interacting proteins involved in endocytosis of the mcc component in aspergillus oryzae. | we have investigated the functions of three endocytosis-related proteins in the filamentous fungus aspergillus oryzae. yeast two-hybrid screening using the endocytic marker protein aoabp1 (a.oryzae homolog of saccharomyces cerevisiae abp1p) as a bait identified four interacting proteins named aip (aoabp1 interacting proteins). in mature hyphae, egfp (enhanced green fluorescent protein) fused to aips colocalized with aoabp1 at the hyphal tip region and the plasma membrane, suggesting that aips fu ... | 2013 | 23597630 |
| allergens causing occupational asthma: an evidence-based evaluation of the literature. | the aim of this work is to provide an evidence-based evaluation and overview of causative substances in order to improve disease management. | 2014 | 23595938 |
| [effect of n-terminal disulfide bridge on thermostability of family 11 xylanases]. | a mesophilic xylanase from aspergillus oryzae, abbreviated to aoxyn11a, belongs to glycoside hydrolase family 11. using aoxyn11a as the parent, the thermotolerant hybrid xylanase, we constructed aex11a by substituting its n-terminus with the corresponding region of a hyperthermostable family 11 xylanase, evxyn11(ts). aoxyn11a- and aex11a-encoding genes were expressed in pichia pastoris gs115 separately, and effects of temperatures on expressed products were determined and compared. the optimum t ... | 2012 | 23593868 |
| food waste as nutrient source in heterotrophic microalgae cultivation. | glucose, free amino nitrogen (fan), and phosphate were recovered from food waste by fungal hydrolysis using aspergillus awamori and aspergillus oryzae. using 100g food waste (dry weight), 31.9 g glucose, 0.28 g fan, and 0.38 g phosphate were recovered after 24h of hydrolysis. the pure hydrolysate has then been used as culture medium and nutrient source for the two heterotrophic microalgae schizochytrium mangrovei and chlorella pyrenoidosa, s. mangrovei and c. pyrenoidosa grew well on the complex ... | 2013 | 23587816 |
| high-level extracellular production and characterization of candida antarctica lipase b in pichia pastoris. | the gene encoding lipase b from candida antarctica (calb) was expressed in pichia pastoris after it was synthesized by the recursive pcr and cloned into the pichia expression plasmid, ppiczαa. the calb was successfully secreted in the recombinant p. pastoris strain x-33 with an apparent molecular weight of 34 kda. for 140 h flask culture, the dry cell weight and the extracellular lipase activity reached at 5.4 g/l and 57.9 u/l toward p-nitrophenyl palmitate, respectively. when we performed the f ... | 2013 | 23571105 |
| isolation and characterization of a proteinaceous antifungal compound from lactobacillus plantarum yml007 and its application as a food preservative. | korean kimchi is known for its myriad of lactic acid bacteria (lab) with diverse bioactive compounds. this study was undertaken to isolate an efficient antifungal lab strain among the isolated kimchi labs. one thousand and four hundred labs isolated from different kimchi samples were initially screened against aspergillus niger. the strain exhibiting the highest antifungal activity was identified as lactobacillus plantarum yml007 by 16s rrna sequencing and biochemical assays using api 50 chl kit ... | 2013 | 23565693 |
| time-dependent correlation of the microbial community and the metabolomics of traditional barley nuruk starter fermentation. | the microbial community and the metabolites of barley nuruk were studied to determine the time-dependent correlation between the fermentation of microbes and metabolites. samples were analyzed by a polyphasic approach based on culture-dependent, culture-independent (pcr-dgge and qpcr analysis), and metabolite analysis using gc-ms. barley nuruk consists of varying amounts of bacteria, yeasts, and molds. the pcr-dgge results showed that only one phylotype, aspergillus oryzae, was predominant throu ... | 2013 | 23563559 |
| inhibition kinetics of lipid oxidation of model foods by using antioxidant extract of fermented soybeans. | fermentation by using aspergillus oryzae has been reported to increase antioxidant activity of soybeans significantly. the effectiveness of the extract from fermented soybeans was studied in 3 model foods with different complexities, i.e., linoleic acid emulsion, sunflower oil emulsions and bulk sunflower oil. for the emulsion systems, oxidation at two different ph values (4.5 and 7) was also compared. a reparameterised logistic equation was used to describe and to predict the experimental data. ... | 2013 | 23561179 |
| identification of telomerase rnas from filamentous fungi reveals conservation with vertebrates and yeasts. | telomeres are the nucleoprotein complexes at eukaryotic chromosomal ends. telomeric dna is synthesized by the ribonucleoprotein telomerase, which comprises a telomerase reverse transcriptase (tert) and a telomerase rna (ter). ter contains a template for telomeric dna synthesis. filamentous fungi possess extremely short and tightly regulated telomeres. although tert is well conserved between most organisms, ter is highly divergent and thus difficult to identify. in order to identify the ter seque ... | 2013 | 23555591 |
| enhanced thermostability of a mesophilic xylanase by n-terminal replacement designed by molecular dynamics simulation. | xylanases have attracted much attention owing to their potential applications. the applicability of xylanases, however, was bottlenecked by their low stabilities at high temperature or extreme ph. the purpose of this work was to enhance the thermostability of a mesophilic xylanase by n-terminal replacement. | 2013 | 23512640 |
| further enhanced production of heterologous proteins by double-gene disruption (δaosedd δaovps10) in a hyper-producing mutant of aspergillus oryzae. | the filamentous fungus aspergillus oryzae is used as one of the most favored hosts for heterologous protein production due to its ability to secrete large amounts of proteins into the culture medium. we previously generated a hyper-producing mutant strain of a. oryzae, aut1, which produced 3.2- and 2.6-fold higher levels of bovine chymosin (chy) and human lysozyme (hly), respectively, compared with the wild-type strain. however, further enhancement of heterologous protein production by multiple ... | 2013 | 23504109 |