Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| conserved and varied dinucleotide sequences in the genomes of three aspergillus species. | both guanine-cytosine content and nucleosome occupancy are higher in exons than in introns. in this study, the association between the frequencies of the dinucleotide sequences and the nucleosome occupancy of the exons and introns of the genes of aspergillus fumigatus, aspergillus nidulans, and aspergillus oryzae was studied. the frequency of the dinucleotide sequences aa (tt), at, and ta in the introns was more than that in the exons. in addition, the frequency of these sequences in the regions ... | 2014 | 25564023 |
| cloning and expression of a novel prolyl endopeptidase from aspergillus oryzae and its application in beer stabilization. | a novel prolyl endopeptidase gene from aspergillus oryzae was cloned and expressed in pichia pastoris. amino acid sequence analysis of the prolyl endopeptidase from aspergillus oryzae (ao-pep) showed that this enzyme belongs to a class serine peptide s28 family. expression, purification and characterization of ao-pep were analyzed. the optimum ph and temperature were ph 5.0 and 40 °c, respectively. the enzyme was activated and stabilized by metal ion ca(2+) and inhibited by zn(2+), mn(2+), al(3+ ... | 2015 | 25547787 |
| dietary supplementation with bacillus subtilis, saccharomyces cerevisiae and aspergillus oryzae enhance immunity and disease resistance against aeromonas hydrophila and streptococcus iniae infection in juvenile tilapia oreochromis niloticus. | a feeding trial was conducted to investigate the effects of dietary administration of probiotic with bacillus subtilis, aspergillus oryzae and saccharomyces cerevisiae on growth, innate immune response, hemato-immunological parameters and disease resistance of nile tilapia, oreochromis niloticus. animals were distributed in three equal groups, each of five replicates and received one of the following experimental diets for four weeks: control, non-supplemented diet; 5 g kg(-1) probiotic mixture ... | 2015 | 25530581 |
| development of aspergillus protease with ovalbumin-induced allergic chronic rhinosinusitis model in the mouse. | chronic rhinosinusitis (crs) is a multifactorial inflammatory disease. particularly, eosinophilic crs is often recalcitrant to treatment, so an appropriate animal model is required to evaluate the pathogenesis of, and to develop therapies for, recalcitrant eosinophilic crs. this study aimed to improve the ovalbumin (ova)-induced mouse model of eosinophilic/allergic crs by combining ova with aspergillus protease, which is known to trigger allergic reactions in mouse lungs. | 2014 | 25514482 |
| purification, cloning, characterization, and n-glycosylation analysis of a novel β-fructosidase from aspergillus oryzae fs4 synthesizing levan- and neolevan-type fructooligosaccharides. | β-fructosidases are a widespread group of enzymes that catalyze the hydrolysis of terminal fructosyl units from various substrates. these enzymes also exhibit transglycosylation activity when they function with high concentrations of sucrose, which is used to synthesize fructooligosaccharides (fos) in the food industry. a β-fructosidase (bfra) with high transglycosylation activity was purified from aspergillus oryzae fs4 as a monomeric glycoprotein. compared with the most extensively studied asp ... | 2014 | 25501957 |
| change in enzyme production by gradually drying culture substrate during solid-state fermentation. | the influence of drying the culture substrate during solid-state fermentation on enzyme production was investigated using a non-airflow box. the drying caused a significant increase in enzyme production, while the mycelium content decreased slightly. this suggests that changes in the water content in the substrate during culture affect enzyme production in fungi. | 2015 | 25488496 |
| distinct mechanism of activation of two transcription factors, amyr and malr, involved in amylolytic enzyme production in aspergillus oryzae. | the production of amylolytic enzymes in aspergillus oryzae is induced in the presence of starch or maltose, and two zn2cys6-type transcription factors, amyr and malr, are involved in this regulation. amyr directly regulates the expression of amylase genes, and malr controls the expression of maltose-utilizing (mal) cluster genes. deletion of malr gene resulted in poor growth on starch medium and reduction in α-amylase production level. to elucidate the activation mechanisms of these two transcri ... | 2015 | 25487891 |
| the ctng gene encodes carbonic anhydrase involved in mycotoxin citrinin biosynthesis from monascus aurantiacus. | citrinin, a fungal secondary metabolite of polyketide origin, is moderately nephrotoxic to vertebrates, including humans. citrinin is synthesised by condensation of acetyl-coa and malonyl-coa. six genes involved in the citrinin biosynthesis, including pksct, ctna and ctnb, have been cloned in monascus purpureus. the pksct gene encodes a polyketide synthase; ctna is a regulatory factor; and ctnb encodes an oxidoreductase. when the three genes were respectively disrupted, the disruption strains dr ... | 2015 | 25482072 |
| effect of the dose of exogenous fibrolytic enzyme preparations on preingestive fiber hydrolysis, ruminal fermentation, and in vitro digestibility of bermudagrass haylage. | our objectives were to evaluate the effects of the dose rates of 5 trichoderma reesei and aspergillus oryzae exogenous fibrolytic enzymes (efe; 1a, 2a, 11c, 13d, and 15d) on in vitro digestibility, fermentation characteristics, and preingestive hydrolysis of bermudagrass haylage and to identify the optimal dose of each efe for subsequent in vitro and in vivo studies. in experiment 1, efe were diluted in citrate-phosphate buffer (ph 6) and applied in quadruplicate in each of 2 runs at 0× (control ... | 2015 | 25468699 |
| molecular characterization of intergeneric hybrid between aspergillus oryzae and trichoderma harzianum by protoplast fusion. | protoplast fusion between aspergillus oryzae and trichoderma harzianum and application of fusant in degradation of shellfish waste. | 2015 | 25444252 |
| purification and characterisation of α-amylase produced by mutant strain of aspergillus oryzae ems-18. | α-amylase produced by a mutant strain of aspergillus oryzae ems-18 has been purified to homogeneity as judged by sodium dodecyle sulphate polyacrylamide gel electrophoresis (sds-page). the enzyme was purified by using 70% ammonium sulphate precipitation followed by anion exchange chromatography on deae-sephadex column and gel filtration on sephadex g-100. an enzyme purification factor of 9.5-fold was achieved with a final specific activity of 1987.7 u/mg protein and overall yield of 23.8%. the m ... | 2015 | 25424747 |
| characterisation of a recombinant β-xylosidase (xyla) from aspergillus oryzae expressed in pichia pastoris. | β-xylosidases catalyse the hydrolysis of short chain xylooligosaccharides from their non-reducing ends into xylose. in this study we report the heterologous expression of aspergillus oryzae β-xylosidase (xyla) in pichia pastoris under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter. the recombinant enzyme was optimally active at 55°c and ph 4.5 with km and vmax values of 1.0 mm and 250 μmol min(-1) mg(-1) respectively against 4-nitrophenyl β-xylopyranoside. xylose was a comp ... | 2014 | 25401069 |
| modulating endoplasmic reticulum-golgi cargo receptors for improving secretion of carrier-fused heterologous proteins in the filamentous fungus aspergillus oryzae. | filamentous fungi are excellent hosts for industrial protein production due to their superior secretory capacity; however, the yield of heterologous eukaryotic proteins is generally lower than that of fungal or endogenous proteins. although activating protein folding machinery in the endoplasmic reticulum (er) improves the yield, the importance of intracellular transport machinery for heterologous protein secretion is poorly understood. here, using aspergillus oryzae as a model filamentous fungu ... | 2015 | 25362068 |
| isolation and expression of enolase gene in fusarium oxysporum f. sp. lycopersici. | fusarium oxysporum f. sp. lycopersici is a fungus responsible for the tomato disease known as fusariosis. enolase, which is the enzyme that catalyzes the reaction of 2-phosphoglycerate to phosphoenolpyruvate, is present during glycolysis. enolase genes have been isolated from bacteria and fungi, among other organisms. in this research, a large portion of the enolase, eno, gene sequence was isolated from f. oxysporum and compared with those of other microorganisms, revealing a similarity of 51-69 ... | 2015 | 25349089 |
| characterization of the production of biogenic amines and gamma-aminobutyric acid in the soybean pastes fermented by aspergillus oryzae and lactobacillus brevis. | the production of gamma-aminobutyric acid (gaba) using gaba-producing lactic acid bacteria (lab) has been considered to be an attractive strategy. however, some lab may produce biogenic amines (ba), which may be of concern from the safety viewpoint. the aim of the present study was to characterize the production of gaba and ba in the soybean pastes fermented by aspergillus oryzae (a. oryzae) fmb s46471 and gaba-producing lactobacillus brevis (l. brevis) gaba 100. after a ripening period of 90 da ... | 2015 | 25341471 |
| evaluation of γ- aminobutyric acid, phytate and antioxidant activity of tempeh-like fermented oats (avena sativa l.) prepared with different filamentous fungi. | tempeh is a popular traditional fermented food in asia. many tempeh-like foods are made from cereal grains. however, the information of γ-aminobutyric acid (gaba) accumulation in those tempeh-like cereal grains during fermentation is lacking. meanwhile, little information is available on the anti-nutrient contents and antioxidant activity of tempeh-like fermented oats. the aim of the present work was to study the changes of gaba, phytate, natural antioxidants and antioxidant activity of tempeh-l ... | 2014 | 25328194 |
| l-lactic acid production from starch by simultaneous saccharification and fermentation in a genetically engineered aspergillus oryzae pure culture. | lactic acid is a commodity chemical that can be produced biologically. lactic acid-producing aspergillus oryzae strains were constructed by genetic engineering. the a. oryzae ldh strain with the bovine l-lactate dehydrogenase gene produced 38 g/l of lactate from 100g/l of glucose. disruption of the wild-type lactate dehydrogenase gene in a. oryzae ldh improved lactate production. the resulting strain a. oryzae ldhδ871 produced 49 g/l of lactate from 100g/l of glucose. because a. oryzae strains i ... | 2014 | 25314668 |
| effects of koji-making with mixed strains on physicochemical and sensory properties of chinese-type soy sauce. | two kinds of soy sauces were prepared with aspergillus oryzae koji (ssao) and mixed koji (ssaom, a. oryzae mouldstarter:monascus purpureus mouldstarter = 1:2, w/w) respectively. the effects of mixed koji on the essential indices, oxygen radical absorption capacity, color indices, free amino acids and volatile compounds of soy sauce have been studied, followed by a sensory evaluation between ssao and ssaom. | 2015 | 25312834 |
| development of a gin11/frt-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicelluloses. | bioethanol produced by the yeast saccharomyces cerevisiae is currently one of the most promising alternatives to conventional transport fuels. lignocellulosic hemicelluloses obtained after hydrothermal pretreatment are important feedstock for bioethanol production. however, hemicellulosic materials cannot be directly fermented by yeast: xylan backbone of hemicelluloses must first be hydrolyzed by heterologous hemicellulases to release xylose, and the yeast must then ferment xylose in the presenc ... | 2014 | 25306430 |
| biosynthesis of the halogenated mycotoxin aspirochlorine in koji mold involves a cryptic amino acid conversion. | aspirochlorine (1) is an epidithiodiketopiperazine (etp) toxin produced from koji mold (aspergillus oryzae), which has been used in the oriental cuisine for over two millennia. considering its potential risk for food safety, we have elucidated the molecular basis of aspirochlorine biosynthesis. by a combination of genetic and chemical analyses we found the acl gene locus and identified the key role of aclh as a chlorinase. stable isotope labeling, biotransformation, and mutational experiments, a ... | 2014 | 25302411 |
| fungal hemicellulose-degrading enzymes cause physical property changes concomitant with solubilization of cell wall polysaccharides. | physical properties of wheat coleoptile segments decreased after treatment with hemicellulose-degrading enzymes, indicating that hemicellulosic polysaccharides function to control the strength of primary cell walls. changes in the physical properties of plant cell walls, a viscoelastic structure, are thought to be one of the growth-limiting factors for plants and one of the infection-affecting factors for fungi. to study the significance of hemicellulosic polysaccharides that form cross-bridges ... | 2015 | 25301670 |
| structure-activity relationship of highly potent galactonoamidine inhibitors toward β-galactosidase (aspergillus oryzae). | a small library of 22 n-substituted galactonoamidines was synthesized, and their structure-activity relationship for inhibition of the hydrolytic activity of β-galactosidase (aspergillus oryzae) was evaluated. a fast screening assay in 96-well plate format was used to follow the enzymatic hydrolysis of 2-chloro-4-nitrophenyl-β-d-galactopyranoside using uv-vis spectroscopy. the aglycon moiety of all compounds was found to have a profound effect on their inhibitory ability. in general, galactonoam ... | 2014 | 25295392 |
| identification of functional cis-elements required for repression of the taka-amylase a gene under secretion stress in aspergillus oryzae. | the expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (ress). to investigate the ress mechanism, we analyzed the expression of the taka-amylase a gene (amyb) in aspergillus oryzae, which was depressed under secreted protein stress. we conducted a truncation and deletion analysis of the amyb promoter to identify cis-elements required for ress. a nucleotide sequ ... | 2015 | 25280730 |
| involvement of hydrophobic amino acid residues in c7-c8 loop of aspergillus oryzae hydrophobin rola in hydrophobic interaction between rola and a polyester. | hydrophobins are amphipathic secretory proteins with eight conserved cysteine residues and are ubiquitous among filamentous fungi. the cys3-cys4 and cys7-cys8 loops of hydrophobins are thought to form hydrophobic segments involved in adsorption of hydrophobins on hydrophobic surfaces. when the fungus aspergillus oryzae is grown in a liquid medium containing the polyester polybutylene succinate-co-adipate (pbsa), a. oryzae produces hydrophobin rola, which attaches to pbsa. here, we analyzed the k ... | 2014 | 25273133 |
| engineering aspergillus oryzae a-4 through the chromosomal insertion of foreign cellulase expression cassette to improve conversion of cellulosic biomass into lipids. | a genetic modification scheme was designed for aspergillus oryzae a-4, a natural cellulosic lipids producer, to enhance its lipid production from biomass by putting the spotlight on improving cellulase secretion. four cellulase genes were separately expressed in a-4 under the control of hlya promoter, with the help of the successful development of a chromosomal genetic manipulation system. comparison of cellulase activities of pcr-positive transformants showed that these transformants integrated ... | 2014 | 25251435 |
| synthesis, characterization and antifungal activity of chemically and fungal-produced silver nanoparticles against trichophyton rubrum. | to characterize and explore the potential in extracellular biosynthesis of silver nanoparticles (agnps) by penicillium chrysogenum and aspergillus oryzae and to investigate the antifungal effect of chemically vs biologically synthesized agnps comparing with conventional antifungal drugs against trichophyton rubrum. | 2014 | 25234047 |
| establishment of a new method to quantitatively evaluate hyphal fusion ability in aspergillus oryzae. | hyphal fusion is involved in the formation of an interconnected colony in filamentous fungi, and it is the first process in sexual/parasexual reproduction. however, it was difficult to evaluate hyphal fusion efficiency due to the low frequency in aspergillus oryzae in spite of its industrial significance. here, we established a method to quantitatively evaluate the hyphal fusion ability of a. oryzae with mixed culture of two different auxotrophic strains, where the ratio of heterokaryotic conidi ... | 2014 | 25229867 |
| effects of fermented corni fructus and fermented kelp on growth performance, meat quality, and emission of ammonia and hydrogen sulphide from broiler chicken droppings. | 1. corni fructus is the fruit of cornus officinalis, a dogwood species. this study was conducted to prepare fermented corni fructus preparation (fcfp) and fermented kelp (fk) from corni fructus and by-products of laminaria japonica fermented with bacillus subtilis and aspergillus oryzae. 2. the effects of dietary fcfp and fk as replacer of oxytetracycline (otc) on growth performance, meat composition, meat oxidative stability, and emissions of ammonia (nh3) and hydrogen sulphide (h2s) from broil ... | 2014 | 25204489 |
| phylogenetic and comparative sequence analysis of thermostable alpha amylases of kingdom archea, prokaryotes and eukaryotes. | alpha amylase family is generally defined as a group of enzymes that can hydrolyse and transglycosylase α-(1, 4) or α-(1, 6) glycosidic bonds along with the preservation of anomeric configuration. for the comparative analysis of alpha amylase family, nucleotide sequences of seven thermo stable organisms of kingdom archea i.e. pyrococcus furiosus (100-105°c), kingdom prokaryotes i.e. bacillus licheniformis (90-95°c), geobacillus stearothermophilus (75°c), bacillus amyloliquefaciens (72°c), bacill ... | 2014 | 25187685 |
| fungal type iii polyketide synthases. | this article covers the literature on fungal type iii polyketide synthases (pkss) published from 2005 to 2014. since the first discovery of fungal type iii pks genes in aspergillus oryzae, reported in 2005, putative genes for type iii pkss have been discovered in fungal genomes. compared with type i pkss, type iii pkss are much less abundant in fungi. however, type iii pkss could have some critical roles in fungi. this article summarizes the studies on fungal type iii pks functional analysis, in ... | 2014 | 25182423 |
| effects of aspergillus oryzae culture and 2-hydroxy-4-(methylthio)-butanoic acid on in vitro rumen fermentation and microbial populations between different roughage sources. | an in vitro experiment was conducted to evaluate the effects of aspergillus oryzae culture (aoc) and 2-hydroxy-4-(methylthio)-butanoic acid (hmb) on rumen fermentation and microbial populations between different roughage sources. two roughage sources (chinese wild rye [cwr] vs corn silage [cs]) were assigned in a 2×3 factorial arrangement with hmb (0 or 15 mg) and aoc (0, 3, or 6 mg). gas production (gp), microbial protein (mcp) and total volatile fatty acid (vfa) were increased in response to a ... | 2014 | 25178372 |
| mixed food waste as renewable feedstock in succinic acid fermentation. | mixed food waste, which was directly collected from restaurants without pretreatments, was used as a valuable feedstock in succinic acid (sa) fermentation in the present study. commercial enzymes and crude enzymes produced from aspergillus awamori and aspergillus oryzae were separately used in hydrolysis of food waste, and their resultant hydrolysates were evaluated. for hydrolysis using the fungal mixture comprising a. awamori and a. oryzae, a nutrient-complete food waste hydrolysate was genera ... | 2014 | 25149459 |
| biotransformation of the mycotoxin zearalenone by fungi of the genera rhizopus and aspergillus. | zearalenone (zen) is a nonsteroidal estrogenic mycotoxin biosynthesized by various fusarium fungi. these fungal species frequently infest grains; therefore, zen represents a common contaminant in cereal products. the biotransformation of zen differs significantly from species to species, and several metabolites are known to be formed by animals, plants, and microorganisms. the aim of the present study was to investigate the microbial conversion of zen by species of the genera rhizopus and asperg ... | 2014 | 25145804 |
| diversity in mrna expression of the serine-type carboxypeptidase ocpg in aspergillus oryzae through intron retention. | alternative splicing is thought to be a means for diversification of products by mrna modification. although some intron retentions are predicted by transcriptome analysis in aspergillus oryzae, its physiological significance remains unknown. we found that intron retention occurred occasionally in the serine-type carboxypeptidase gene, ocpg. analysis under various culture conditions revealed that extracellular nitrogen conditions influence splicing patterns; this suggested that there might be a ... | 2014 | 25130734 |
| structural analysis of cerebrosides from aspergillus fungi: the existence of galactosylceramide in a. oryzae. | glucosylceramide and galactosylceramide were detected in three aspergillus species: aspergillus oryzae, aspergillus sojae and aspergillus. awamori, using borate-coated tlc. the cerebrosides from a. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determined the composition of sugar, fatty acid and sphingoid base by gc/ms, maldi-tof/ms and (1)h-nmr. we identified them as β-glucosylceramide and β-galactosylceramide. the ceramide moiety ... | 2014 | 25129050 |
| c7-prenylation of tryptophanyl and o-prenylation of tyrosyl residues in dipeptides by an aspergillus terreus prenyltransferase. | during our search for novel prenyltransferases, a putative gene ateg_04218 from aspergillus terreus raised our attention and was therefore amplified from strain dsm 1958 and expressed in escherichia coli. biochemical investigations with the purified recombinant protein and different aromatic substrates in the presence of dimethylallyl diphosphate revealed the acceptance of all the tested tryptophan-containing cyclic dipeptides. structure elucidation of the main enzyme products by nmr and ms anal ... | 2015 | 25125042 |
| cloning and expression of a. oryzae β-glucosidase in pichia pastoris. | a β-glucosidase gene (bgl) from aspergillus oryzae gif-10 was cloned, sequenced and expressed. its full-length dna sequence was 2,903 bp and included three introns. the full-length cdna sequence contained an open reading frame of 2,586 nucleotides, encoding 862 amino acids with a potential secretion signal. the a. oryzae gif-10 bgl was functionally expressed in pichia pastoris. after 7-day induction, protein yield reached 321 mg/ml. using salicin as the substrate, the specific activity of the pu ... | 2014 | 25123895 |
| improvement of aspergillus oryzae nrrl 3484 by mutagenesis and optimization of culture conditions in solid-state fermentation for the hyper-production of extracellular cellulase. | spore suspensions of aspergillus oryzae nrrl 3484 were subjected to mutagenesis using ultraviolet-irradiation followed by chemical treatments to improve the biosynthesis of cellulase. ten mutant strains namely ueac7, uear5, unac4, unac16, unar19, unbc7, unbr3, unbr10, unbr23 and unbr25 were selected and their extracellular cellulase activities were assayed. mutant unac4 gave the highest cellulase production [2,455 ± 28 u/g-dry substrate (ds) for filter paper-ase (fp-ase)] in a yield 4-fold excee ... | 2014 | 25119245 |
| fungal lectins: a growing family. | fungi are members of a large group of eukaryotic organisms that include yeasts and molds, as well as the most familiar member, mushrooms. fungal lectins with unique specificity and structures have been discovered. in general, fungal lectins are classified into specific families based on their amino acid sequences and three-dimensional structures. in this chapter, we provide an overview of the approximately 80 types of mushroom and fungal lectins that have been isolated and studied to date. in pa ... | 2014 | 25117221 |
| optimization of the condition for adsorption of gallic acid by aspergillus oryzae mycelia using box-behnken design. | fresh biomass of aspergillus oryzae (a. oryzae) cgmcc5992 can effectively remove gallic acid from aqueous solution. to improve the removal rate of gallic acid, this study first identified the important factors affecting the removal rate of gallic acid with univariate analysis, and then used four-factor and three-level box-behnken design (bbd) with the removal rate of gallic acid as response value, to obtain the optimum conditions for the removal of gallic acid as follows: 6.95 h treatment time, ... | 2015 | 25109471 |
| a matter of structure: structural comparison of fungal carbonic anhydrases. | carbonic anhydrases (cas) are metalloenzymes that catalyze the interconversion of carbon dioxide (co2) and hydrogen carbonate. cas are distributed over all the three domains of life and are divided into five distinct evolutionarily unrelated gene families (α, β, γ, δ, ζ). in the large fungal kingdom, the majority of fungi encode multiple copies of β-cas, with some also possessing genes for α-class cas. hemiascomycetous and basidiomycetous yeasts encode one or two β-cas, while most of the filamen ... | 2014 | 25109265 |
| [the effect of enzymatic treatment using proteases on properties of persistent sodium current in ca1 pyramidal neurons of rat hippocampus]. | we investigated the effect of proteases, widely used for neuron isolation in electrophysiological studies, on the amplitude and kinetic characteristics of persistent sodium current (i(nap)) in hippocampal ca1 pyramidal neurons. properties of i(nap) were studied on neurons isolated by mechanical treatment (control group) and by mechanical and enzymatic treatment using pronase e (from streptomyces griseus) or protease type xxiii (from aspergillus oryzae). we show that in neurons isolated with pron ... | 2014 | 25097934 |
| rapid reconstitution of biosynthetic machinery for fungal metabolites in aspergillus oryzae: total biosynthesis of aflatrem. | reconstitution of the biosynthetic machinery for fungal secondary metabolites in aspergillus oryzae provides an opportunity both for stepwise determination of the biosynthetic pathways and the total biosynthesis of fungal natural products. however, to maximize the utility of the reconstitution system, a simple and rapid strategy for the introduction of heterologous genes into a. oryzae is required. in this study, we demonstrated an effective method for introducing multiple genes involved in the ... | 2014 | 25087641 |
| expression of key hydrolases for soy sauce fermentation in zygosaccharomyces rouxii. | several key hydrolases in soy sauce fermentation such as proteases, peptidases, and glutaminases are supplied by aspergillus sojae or aspergillus oryzae. the genes encoding these hydrolases were successfully expressed in salt-tolerant yeast zygosaccharomyces rouxii. these transformants are expected to supply extra hydrolases during soy sauce fermentation process. | 2015 | 25073685 |
| crystal structure of a feruloyl esterase belonging to the tannase family: a disulfide bond near a catalytic triad. | feruloyl esterase (fae) catalyzes the hydrolysis of the ferulic and diferulic acids present in plant cell wall polysaccharides, and tannase catalyzes the hydrolysis of tannins to release gallic acid. the fungal tannase family in the esther database contains various enzymes, including faes and tannases. despite the importance of faes and tannases in bioindustrial applications, three-dimensional structures of the fungal tannase family members have been unknown. here, we determined the crystal stru ... | 2014 | 25066066 |
| gas chromatography/mass spectrometry based component profiling and quality prediction for japanese sake. | sake is a japanese traditional alcoholic beverage, which is produced by simultaneous saccharification and alcohol fermentation of polished and steamed rice by aspergillus oryzae and saccharomyces cerevisiae. about 300 compounds have been identified in sake, and the contribution of individual components to the sake flavor has been examined at the same time. however, only a few compounds could explain the characteristics alone and most of the attributes still remain unclear. the purpose of this st ... | 2014 | 25060729 |
| effects of dietary fermented seaweed and seaweed fusiforme on growth performance, carcass parameters and immunoglobulin concentration in broiler chicks. | this study was conducted to investigate the effects of brown seaweed (undaria pinnatifida) by-product and seaweed fusiforme (hizikia fusiformis) by-product supplementation on growth performance and blood profiles including serum immunoglobulin (ig) in broilers. fermentation of seaweeds was conducted by bacillus subtilis and aspergillus oryzae. in a 5-wk feeding trial, 750 one-d-old broiler chicks were divided into 5 groups, and were assigned to the control diet or experimental diets including co ... | 2014 | 25050025 |
| comparison of the genomes and transcriptomes associated with the different protease secretions of aspergillus oryzae 100-8 and 3.042. | aspergillus oryzae is used to produce traditional fermented foods and beverages. a. oryzae 3.042 produces a neutral protease and an alkaline protease but rarely an acid protease, which is unfavourable to soy-sauce fermentation. a. oryzae 100-8 was obtained by n(+) ion implantation mutagenesis of a. oryzae 3.042, and the protease secretions of these two strains are different. sequencing the genome of a. oryzae 100-8 and comparing it to the genomes of a. oryzae 100-8 and 3.042 revealed some differ ... | 2014 | 25048221 |
| a combination of acid lactase from aspergillus oryzae and yogurt bacteria improves lactose digestion in lactose maldigesters synergistically: a randomized, controlled, double-blind cross-over trial. | lactose digestion can be improved in subjects with impaired or completely absent intestinal lactase activity by administration of lactase preparations and particularly of acid lactase, which is active in the stomach, or by yogurt containing live lactic acid bacteria. it is the question, if lactose digestion can be further enhanced by combining these two approaches. | 2015 | 25042846 |
| trichoderma reesei xyn vi--a novel appendage-dependent eukaryotic glucuronoxylan hydrolase. | expression of a trichoderma reesei gene coding for a putative gh30 xylanase in aspergillus oryzae led to isolation and purification of a novel xylanase exhibiting catalytic properties different from those of the previously characterized gh30 xylanase xyn iv of t. reesei. the novel enzyme, named xyn vi, exhibited catalytic properties similar to appendage-dependent gh30 glucuronoxylanases previously recognized only in bacteria. xyn vi showed high specific activity only on xylans or xylooligosaccha ... | 2014 | 25041335 |
| plant-available inorganic nutrient levels are increased in rice-derived distillery effluents inoculated with microbes. | the purpose of this study was to assess the effects of microbes on plant-available inorganic nutrients and a phytohormone in rice-derived distillery effluents. | 2014 | 25040155 |
| targeted metabolomics for aspergillus oryzae-mediated biotransformation of soybean isoflavones, showing variations in primary metabolites. | this study aimed to investigate the biotransformation of soybean isoflavones to hydroxyisoflavones, and the primary and secondary metabolite change during aspergillus oryzae kacc40247-mediated fermentation by gas chromatography-time of flight-mass spectrometry and lc-ms with multivariate analysis. the mass spectrometric analysis revealed that acetylglycosides and glycosides decreased during the first 12 h of fermentation, while the aglycones increased up to that time point. this was followed by ... | 2014 | 25036500 |
| telomere-mediated chromosomal truncation in aspergillus oryzae. | we truncated the short arm of chromosome 3 to delete the aflatoxin biosynthesis gene homolog cluster using telomeric repeats in aspergillus oryzae. the predicted deletion was confirmed by southern blot analyses. this telomere-mediated chromosomal truncation method enables the development of an artificial chromosome in a. oryzae. | 2015 | 25034635 |
| a comparative study on the degradation of gallic acid by aspergillus oryzae and phanerochaete chrysosporium. | recently, as an emerging persistent dissolved organic pollutant (dop), gallic acid (ga) and its efficient decomposition methods have received global attention. the present work aimed to compare the effect of aspergillus oryzae 5992 and phanerochaete chrysosporium 40719 on degradation of different concentrations of ga. the a. oryzae grew well and achieved a ga removal rate up to 99% in media containing 1-4% ga, much higher than p. chrysosporium. the activity of laccase and lignin peroxidase excre ... | 2014 | 25026596 |
| [gene cloning, expression of a feruloyl esterase a and purification of its hydrolysis products]. | to express feruloyl esterase a from aspergillus oryzae in pichia pastoris expression system and study its hydrolysis function, explore the conditions and effects of purification for ferulic acid extracts by macroporos resin. using the total rna from a. oryzae cicc 40186 as the template, we amplified coding sequence aorfaea encoding a mature feruloyl esterase a (aorfaea) by rt-pcr technique. then, the coding sequence aorfaea was successfully expressed in pichia pastoris gs115 mediated by an expre ... | 2014 | 25007578 |
| assessing medium constituents for optimal heterologous production of anhydromevalonolactone in recombinant aspergillus oryzae. | anhydromevalonolactone (amvl) is a bioactive natural product that arises from a molecular biology technique using aspergillus oryzae as a heterologous host. amvl has been used as a precursor for the synthesis of insect pest control reagents and has numerous applications in the biotechnological and medical industries. in this study, the plackett-burman design and the central composite design, which offer efficient and feasible approaches, were complemented to screen significant parameters and ide ... | 2014 | 25006541 |
| safety evaluation of filamentous fungi isolated from industrial doenjang koji. | a few starters have been developed and used for doenjang fermentation but often without safety evaluation. filamentous fungi were isolated from industrial doenjang koji, and their potential for mycotoxin production was evaluated. two fungi were isolated; one was more dominantly present (90%). both greenish (snu-g) and whitish (snu-w) fungi showed 97% and 95% internal transcribed spacer sequence identities to aspergillus oryzae/flavus, respectively. however, the smai digestion pattern of their ge ... | 2014 | 24986679 |
| speradines f-h, three new oxindole alkaloids from the marine-derived fungus aspergillus oryzae. | a rare hexacyclic oxindole alkaloid, speradine f (1), together with two novel tetracyclic oxindole alkaloids, speradines g (2) and h (3), were isolated from the marine-derived fungus aspergillus oryzae. their structures were determined by spectroscopic methods and x-ray diffraction analysis. this study is the first report on cyclopiazonic acid (cpa)-type alkaloids with a hexacyclic skeleton. | 2014 | 24966178 |
| upgrading the antioxidant potential of cereals by their fungal fermentation under solid-state cultivation conditions. | solid-state fermentation (ssf) at 30°c for 72 h with four generally recognized as safe (gras) filamentous fungi (aspergillus oryzae ncim 1212, aspergillus awamori mtcc no. 548, rhizopus oligosporus ncim 1215 and rhizopus oryzae rck2012) showed high efficiency for the improvement of water-soluble total phenolic content (tpc) and antioxidant properties including abts(●+) [2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid)] and dpph(●) (2,2'-diphenyl-1-picrylhydrazyl) scavenging capacities of ... | 2014 | 24964826 |
| supplementation with aspergillus oryzae-fermented kochujang lowers serum cholesterol in subjects with hyperlipidemia. | kochujang, a traditional fermented red pepper paste, is known for its hypocholesterolemic effect; however, these studies used non-commercial preparations of kochujang. in this study, we examined whether commercially-made kochujang in which aspergillus oryzae (also known as koji) was used as a microorganism for fermentation has the same cholesterol-lowering effects. | 2015 | 24961447 |
| genome-scale analysis of the high-efficient protein secretion system of aspergillus oryzae. | the koji mold, aspergillus oryzae is widely used for the production of industrial enzymes due to its particularly high protein secretion capacity and ability to perform post-translational modifications. however, systemic analysis of its secretion system is lacking, generally due to the poorly annotated proteome. | 2014 | 24961398 |
| comparative chemistry of aspergillus oryzae (rib40) and a. flavus (nrrl 3357). | aspergillus oryzae and a. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production. in this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of a. oryzae (rib40) and a. flavus (nrrl 3357). ... | 2012 | 24957367 |
| regioselective synthesis of cytarabine monopropionate by using a fungal whole-cell biocatalyst in nonaqueous medium. | the utilization of a dehydrated fungal biocatalyst of aspergillus oryzae cells was successfully performed to achieve efficient acylation modification of a polar nucleoside cytarabine (ara-c). organic solvents showed evident influence on the reaction catalyzed by the a. oryzae whole-cells. except for hexane-pyridine, the catalytic activity and regioselectivity of the whole-cells clearly increased with increasing the polarity of the hydrophobic organic solvents used. the effects of some crucial fa ... | 2014 | 24957351 |
| biochemical properties of a new cold-active mono- and diacylglycerol lipase from marine member janibacter sp. strain htcc2649. | mono- and di-acylglycerol lipase has been applied to industrial usage in oil modification for its special substrate selectivity. until now, the reported mono- and di-acylglycerol lipases from microorganism are limited, and there is no report on the mono- and di-acylglycerol lipase from bacteria. a predicted lipase (named maj1) from marine janibacter sp. strain htcc2649 was purified and biochemical characterized. maj1 was clustered in the family i.7 of esterase/lipase. the optimum activity of the ... | 2014 | 24927145 |
| expression, purification and crystallization of a fungal type iii polyketide synthase that produces the csypyrones. | csyb from aspergillus oryzae is a novel type iii polyketide synthase that catalyzes the formation of csypyrone b1 [4-(3-acetyl-4-hydroxy-2-oxo-2h-pyran-6-yl)butyric acid] from fatty acyl-coa, malonyl-coa and acetoacetyl-coa. recombinant csyb expressed in escherichia coli was crystallized by the sitting-drop vapour-diffusion method. the crystals belonged to space p2₁, with unit-cell parameters a=70.0, b=104.8, c=73.5 å, β=114.4°. | 2014 | 24915080 |
| draft genome sequence of aspergillus oryzae 100-8, an increased acid protease production strain. | aspergillus oryzae is a common fungus for traditional fermentation in asia, such as spirit, soybean paste, and soy sauce fermentation. we report the 36.7-mbp draft genome sequence of a. oryzae 100-8 and compared it to the published genome sequence of a. oryzae 3.042. | 2014 | 24903875 |
| aspergillus oryzae csyb catalyzes the condensation of two β-ketoacyl-coas to form 3-acetyl-4-hydroxy-6-alkyl-α-pyrone. | the type iii polyketide synthases from fungi produce a variety of secondary metabolites including pyrones, resorcinols, and resorcylic acids. we previously reported that csyb from aspergillus oryzae forms α-pyrone csypyrone b compounds when expressed in a. oryzae. feeding experiments of labeled acetates indicated that a fatty acyl starter is involved in the reaction catalyzed by csyb. here we report the in vivo and in vitro reconstitution analysis of csyb. when csyb was expressed in escherichia ... | 2014 | 24895122 |
| aspergillus oryzae-based cell factory for direct kojic acid production from cellulose. | kojic acid (5-hydroxy-2-(hydroxymethyl)-4-pyrone) is one of the major secondary metabolites in aspergillus oryzae. it is widely used in food, pharmaceuticals, and cosmetics. the production cost, however, is too high for its use in many applications. thus, an efficient and cost-effective kojic acid production process would be valuable. however, little is known about the complete set of genes for kojic acid production. currently, kojic acid is produced from glucose. the efficient production of koj ... | 2014 | 24885968 |
| phytase production by aspergillus oryzae in solid-state fermentation and its applicability in dephytinization of wheat bran [corrected]. | aspergillus oryzae sbs50 secreted a high titre of phytase in solid-state fermentation (ssf) using wheat bran at 30 °c after 96 h at the initial substrate to moisture ratio of 1:2 and a water activity of 0.95. the production of phytase increased when wheat bran was supplemented with sucrose and beef extract. further enhancement in enzyme production was recorded when the substrate was supplemented with the surfactant triton x-100 (145 u/g of dmb). an overall 29-fold improvement in phytase producti ... | 2014 | 24879597 |
| over-expression of a proline specific aminopeptidase from aspergillus oryzae jn-412 and its application in collagen degradation. | a strain that exhibited intracellular proline-specific aminopeptidase (pap) activity was isolated from soy sauce koji and identified as aspergillus oryzae jn-412. the gene coding pap was cloned and efficiently expressed in escherichia coli bl21 in a biologically active form. the highest specific activity reached 52.28 u mg(-1) at optimum cultivation conditions. the recombinant enzyme was purified 3.3-fold to homogeneity with a recovery of 36.7% from cell-free extract using ni-affinity column chr ... | 2014 | 24879594 |
| biocontrol activity of the local strain of metschnikowia pulcherrima on different postharvest pathogens. | the strains of the yeast metschnikowia pulcherrima have strong biocontrol activity against various microorganisms. biocontrol activity of m. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin. biocontrol activity of pulcherrimin producing strain, m. pulcherrima umy15, isolated from local vineyards, was tested on different molds that cause food spoilage. m. pulcherrima umy15 was a very effective biocontrol agent against penicillium roqueforti, p. italicum, p. expansum, and ... | 2014 | 24860671 |
| biochemical characterization of aspergillus oryzae native tannase and the recombinant enzyme expressed in pichia pastoris. | in this study, the biochemical properties of the recombinant tannase from aspegillus oryzae were compared with those of the native enzyme. extracellular native tannase was purified from a commercial enzyme source. recombinant tannase highly expressed in pichia pastoris was prepared as an active extracellular protein. purified native and recombinant tannases produced smeared bands with apparent molecular masses of 45-80 kda and 45-75 kda, respectively, by sodium dodecyl sulfate-polyacrylamide gel ... | 2014 | 24856589 |
| an evaluation of aflatoxin and cyclopiazonic acid production in aspergillus oryzae. | to date, edible fungi such as aspergillus flavus var. oryzae (a. oryzae) has been considered as safe. however, some strains can produce mycotoxins. thus, the biosynthetic ability to produce mycotoxins should be reevaluated to determine the safety of edible fungi. we analyzed the production of aflatoxins and cyclopiazonic acid (cpa) from edible fungi such as a. oryzae isolated from various korean foods using multiplex pcr, enzyme-linked immunosorbent assay, and high-performance liquid chromatogra ... | 2014 | 24853527 |
| targeted tandem duplication of a large chromosomal segment in aspergillus oryzae. | we describe here the first successful construction of a targeted tandem duplication of a large chromosomal segment in aspergillus oryzae. the targeted tandem chromosomal duplication was achieved by using strains that had a 5'-deleted pyrg upstream of the region targeted for tandem chromosomal duplication and a 3'-deleted pyrg downstream of the target region. consequently,strains bearing a 210-kb targeted tandem chromosomal duplication near the centromeric region of chromosome 8 and strains beari ... | 2014 | 24837372 |
| dry entrapment of enzymes by epoxy or polyester resins hardened on different solid supports. | embedding of enzymes was performed with epoxy or polyester resin by mixing in a dried enzyme preparation before polymerization was started. this fast and low-cost immobilization method produced enzymatically active layers on different solid supports. as model enzymes the well-characterized thermomyces lanuginosus lipase and a new threonine aldolase from ashbya gossypii were used. it was shown that t. lanuginosus lipase recombinantly expressed in aspergillus oryzae is a monomeric enzyme with a mo ... | 2014 | 24835099 |
| a large nonconserved region of the tethering protein leashin is involved in regulating the position, movement, and function of woronin bodies in aspergillus oryzae. | the woronin body is a pezizomycotina-specific organelle that is typically tethered to the septum, but upon hyphal wounding, it plugs the septal pore to prevent excessive cytoplasmic loss. leashin (lah) is a large woronin body tethering protein that contains highly conserved n- and c-terminal regions and a long (∼2,500-amino-acid) nonconserved middle region. as the involvement of the nonconserved region in woronin body function has not been investigated, here, we functionally characterized indivi ... | 2014 | 24813188 |
| non-aflatoxigenicity of commercial aspergillus oryzae strains due to genetic defects compared to aflatoxigenic aspergillus flavus. | aspergillus oryzae is generally recognized as safe, but it is closely related to a. flavus in morphology and genetic characteristics. in this study, we tested the aflatoxigenicity and genetic analysis of nine commercial a. oryzae strains that were used in korean soybean fermented products. cultural and hplc analyses showed that none of the commercial strains produced detectable amount of aflatoxins. according to the molecular analysis of 17 genes in the aflatoxin (af) biosynthetic pathway, the c ... | 2014 | 24759426 |
| filamentous fungal diversity and community structure associated with the solid state fermentation of chinese maotai-flavor liquor. | maotai-flavor liquor is produced by simultaneous saccharification and fermentation (ssf) process under solid state conditions, including daqu (starter) making, stacking fermentation and alcohol fermentation stages. filamentous fungi produce many enzymes to degrade the starch material into fermentable sugar during liquor fermentation. this study investigated the filamentous fungal community associated with liquor making process. eight and seven different fungal species were identified by using cu ... | 2014 | 24742997 |
| construction of brewing-wine aspergillus oryzae pyrg- mutant by pyrg gene deletion and its application in homology transformation. | pyrg(-) host cells are indispensable for pyrg(-) based transformation system. isolations of pyrg(-) host cells by random mutations are limited by time-consuming, unclear genetic background and potential interferences of homogenous recombination. the purpose of this study was to construct brewing-wine aspergillus oryzae pyrg(-) mutant by site-directed mutation of pyrg gene deletion which would be used as a host for further transformation. pmd-pyrgab, a vector carrying pyrg deletion cassette, was ... | 2014 | 24742431 |
| motif-independent prediction of a secondary metabolism gene cluster using comparative genomics: application to sequenced genomes of aspergillus and ten other filamentous fungal species. | despite their biological importance, a significant number of genes for secondary metabolite biosynthesis (smb) remain undetected due largely to the fact that they are highly diverse and are not expressed under a variety of cultivation conditions. several software tools including smurf and antismash have been developed to predict fungal smb gene clusters by finding core genes encoding polyketide synthase, nonribosomal peptide synthetase and dimethylallyltryptophan synthase as well as several othe ... | 2014 | 24727546 |
| strain development and optimized fermentation conditions for blood meal using aspergillus niger and aspergillus oryzae. | to hydrolyze blood meal (bm) effectively, two mutants were generated using ultra-violet mutagenesis. single-factor experiments, the plackett-burman technique and response surface methodology were used to optimize the fermentation conditions. this study successfully generated a mutant and innovatively provided important parameters for utilizing bm by fermentation, which could be of industrial value. | 2014 | 24726870 |
| rapid delivery of diazepam from supersaturated solutions prepared using prodrug/enzyme mixtures: toward intranasal treatment of seizure emergencies. | current treatments for seizure emergencies, such as status epilepticus, include intravenous or rectal administration of benzodiazepines. while intranasal delivery of these drugs is desirable, the small volume of the nasal cavity and low drug solubility pose significant difficulties. here, we prepared supersaturated diazepam solutions under physiological conditions and without precipitation, using a prodrug/enzyme system. avizafone, a peptide prodrug of diazepam, was delivered with--aspergillus o ... | 2014 | 24700272 |
| analyzing the relation between the microbial diversity of daqu and the turbidity spoilage of traditional chinese vinegar. | vinegar is a traditional fermented condiment, and the microbial diversity of daqu makes the quality of vinegar products. recently, turbidity spoilage of vinegar sharply tampered with the quality of vinegar. in this study, the relation between the microbial diversity of daqu and turbidity spoilage of vinegar was analyzed by plating technique, pcr-denaturing gradient gel electrophoresis (dgge), and high-performance liquid chromatography (hplc). the 16s rrna sequencing and dgge analysis indicated t ... | 2014 | 24691870 |
| effects of codon optimization on the mrna levels of heterologous genes in filamentous fungi. | filamentous fungi, particularly aspergillus species, have recently attracted attention as host organisms for recombinant protein production. because the secretory yields of heterologous proteins are generally low compared with those of homologous proteins or proteins from closely related fungal species, several strategies to produce substantial amounts of recombinant proteins have been conducted. codon optimization is a powerful tool for improving the production levels of heterologous proteins. ... | 2014 | 24682479 |
| new insights into heat induced structural changes of pectin methylesterase on fluorescence spectroscopy and molecular modeling basis. | heat-induced structural changes of aspergillus oryzae pectin methylesterase (pme) were studied by means of fluorescence spectroscopy and molecular modeling, whereas the functional enzyme stability was monitored by inactivation studies. the fluorescence spectroscopy experiments were performed at two ph value (4.5 and 7.0). at both ph values, the phase diagrams were linear, indicating the presence of two molecular species induced by thermal treatment. a red shift of 7 nm was observed at neutral ph ... | 2014 | 24657465 |
| biorefinery development through utilization of biodiesel industry by-products as sole fermentation feedstock for 1,3-propanediol production. | rapeseed meal (rsm) hydrolysate was evaluated as substitute for commercial nutrient supplements in 1,3-propanediol (pdo) fermentation using the strain clostridium butyricum vpi 1718. rsm was enzymatically converted into a generic fermentation feedstock, enriched in amino acids, peptides and various micro-nutrients, using crude enzyme consortia produced via solid state fermentation by a fungal strain of aspergillus oryzae. initial free amino nitrogen concentration influenced pdo production in bat ... | 2014 | 24650530 |
| discovery and analysis of an active long terminal repeat-retrotransposable element in aspergillus oryzae. | wild-type aspergillus oryzae rib40 contains two copies of the ao090005001597 gene. we previously constructed a. oryzae rib40 strain, rkuaf8b, with multiple chromosomal deletions, in which the ao090005001597 copy number was found to be increased significantly. sequence analysis indicated that ao090005001597 is part of a putative 6,000-bp retrotransposable element, flanked by two long terminal repeats (ltrs) of 669 bp, with characteristics of retroviruses and retrotransposons, and thus designated ... | 2014 | 24646755 |
| antioxidant and antifungal activity of extracts of the aerial parts of thymus capitatus (l.) hoffmanns against four phytopathogenic fungi of citrus sinensis. | many medicinal plants from the lamiaceae family can be easily found in algeria. these plants have been used as traditional medicines by local ethnic groups. thymus capitatus is known in algeria as "zaatar" and has been commonly used as a spice, and reported to have many biological effects. | 2014 | 24644439 |
| trypsin inhibitor from edible mushroom pleurotus floridanus active against proteases of microbial origin. | protease inhibitors can be versatile tools mainly in the fields of medicine, agriculture and food preservative applications. fungi have been recognized as sources of protease inhibitors, although there are only few such reports on mushrooms. this work reports the purification and characterization of a trypsin inhibitor from the fruiting body of edible mushroom pleurotus floridanus (pfti) and its effect on the activity of microbial proteases. the protease inhibitor was purified up to 35-fold by d ... | 2014 | 24615526 |
| preparation of przewalskinic acid a from salvianolic acid b using a crude enzyme from an aspergillus oryzae strain. | przewalskinic acid a is a rare, water-soluble, and highly biologically active ingredient found, thus far, only in the salvia przewalskii maxim herb; however, the content in s. przewalskii herb is very low. in order to obtain useful quantities of przewalskinic acid a, the biotransformatin of salvianolic acid b from salvia miltiorrhiza root (danshen in chinese) into przewalskinic acid a was studied using a crude enzyme produced from aspergillus oryzae d30s strain. the crude enzyme from the a. oryz ... | 2014 | 24615145 |
| functional analysis of histone deacetylase and its role in stress response, drug resistance and solid-state cultivation in aspergillus oryzae. | in the eukaryotic cell, histone deacetylases (hdacs) play key roles in the regulation of fundamental cellular process such as development regulation, stress response, secondary metabolism and genome integrity. here, we provide a comprehensive phenotypic analysis using hdac disruptants in aspergillus oryzae. our study revealed that four hdacs, hdaa/aohda1, hdab/aorpd3, hdad/aohos2 and hst4/aohstd were involved in stress response, cell wall synthesis and chromatin integrity in a. oryzae. osmotic s ... | 2014 | 24613105 |
| process characterization and influence of alternative carbon sources and carbon-to-nitrogen ratio on organic acid production by aspergillus oryzae dsm1863. | l-malic acid and fumaric acid are c4 dicarboxylic organic acids and considered as promising chemical building blocks. they can be applied as food preservatives and acidulants in rust removal and as polymerization starter units. molds of the genus aspergillus are able to produce malic acid in large quantities from glucose and other carbon sources. in order to enhance the production potential of aspergillus oryzae dsm 1863, production and consumption rates in an established bioreactor batch-proces ... | 2014 | 24604500 |
| fungal hydrolysis in submerged fermentation for food waste treatment and fermentation feedstock preparation. | potential of fungal hydrolysis in submerged fermentation by aspergillus awamori and aspergillus oryzae as a food waste treatment process and for preparation of fermentation feedstock has been investigated. by fungal hydrolysis, 80-90% of the initial amount of waste was reduced and degraded within 36-48 h into glucose, free amino nitrogen (fan) and phosphate. experiments revealed that 80-90% of starch can be converted into glucose and highest concentration of fan obtained, when solid mashes of a. ... | 2014 | 24583214 |
| purification and evaluation of the enzymatic properties of a novel fructosyltransferase from aspergillus oryzae: a potential biocatalyst for the synthesis of sucrose 6-acetate. | a novel fructosyltransferase (aoft) capable of synthesizing sucrose 6-acetate (s6a) from sucrose and glucose 6-acetate has been purified to homogeneity from aspergillus oryzae zz-01. its molecular mass was ~50 kda by sds-page; optimal activity was at 45 °c and it was stable from ph 4.5 to 7.5 with an optimum ph of 6. mg(2+), k(+) (5 mm), propanol, toluene (50%, v/v), tween 20 or triton x-100 (1%, w/v) increased the transfructosylation activity by 20, 17, 17, 10, 25 and 20%, respectively. an over ... | 2014 | 24563291 |
| protective effects of the fermented laminaria japonica extract on oxidative damage in llc-pk1 cells. | this study investigated the protective effect of the butanol (buoh) fraction from fermented laminaria japonica extract (bflj) on aaph-induced oxidative stress in porcine kidney epithelial cells (llc-pk1 cells). l. japonica was fermented by aspergillus oryzae at 35±1°c for 72 h. freeze-dried fermented l. japonica was extracted with distilled water, and the extracted solution was mixed with ethanol and then centrifuged. the supernatant was subjected to sequential fractionation with various solvent ... | 2013 | 24551823 |
| trimming of glucosylated n-glycans by human er α1,2-mannosidase i. | in the endoplasmic reticulum (er), folding of proteins modified by asparagine-linked (n-linked) glycosylation is precisely monitored by quality control machinery. upon exit from the calnexin/calreticulin cycle, glycoproteins are digested by α-mannosidases in the er, especially α1,2-mannosidase i (ermani). ermani removes the α1,2-linked mannose of the b-chain from properly folded er glycoproteins, whereas two or more α1,2-linked mannose residues are sequentially trimmed from improperly folded gly ... | 2014 | 24519966 |
| cloning and expression of a family 10 xylanase gene (aoxyn10) from aspergillus oryzae in pichia pastoris. | a full-length cdna sequence of aoxyn10, a gene encoding a glycoside hydrolase (gh) family 10 xylanase from aspergillus oryzae, was amplified from the total rna by 3' and 5' rapid amplification of cdna ends. the cdna sequence is 1,689 bp, containing 5', 3' untranslated regions and a 1,422 bp open reading frame (orf) that encodes a 21-aa signal peptide and a 452-aa mature peptide (designated aoxyn10). multi-alignment revealed that aoxyn10 contains two regions: a catalytic domain (cd) and a family ... | 2013 | 24492599 |
| direct fermentation of raw starch using a kluyveromyces marxianus strain that expresses glucoamylase and alpha-amylase to produce ethanol. | raw starch and raw cassava tuber powder were directly and efficiently fermented at elevated temperatures to produce ethanol using the thermotolerant yeast kluyveromyces marxianus that expresses α-amylase from aspergillus oryzae as well as α-amylase and glucoamylase from debaryomyces occidentalis. among the constructed k. marxianus strains, yrl 009 had the highest efficiency in direct starch fermentation. raw starch from corn, potato, cassava, or wheat can be fermented at temperatures higher than ... | 2014 | 24478139 |
| modes of inhibition of α -amylase and α -glucosidase by aqueous extract of morinda lucida benth leaf. | diabetes mellitus is a metabolic disorder of glucose metabolism. the management of blood glucose level is the hallmark in the treatment of this disease. this may be achieved through the use of oral hypoglycemic drugs such as biguanides, insulin secretagogues, and α-glucosidase inhibitors. the purpose of the present study was to investigate the inhibitory effect of morinda lucida leaf extracts on the activities of α-amylase and α-glucosidase. this was performed using α-amylase from aspergillus or ... | 2013 | 24455701 |
| canola cake as a potential substrate for proteolytic enzymes production by a selected strain of aspergillus oryzae: selection of process conditions and product characterization. | oil cakes have excellent nutritional value and offer considerable potential for use in biotechnological processes that employ solid-state fermentation (ssf) for the production of high value products. this work evaluates the feasibility of using canola cake as a substrate for protease production by a selected strain of aspergillus oryzae cultivated under ssf. the influences of the following process parameters were considered: initial substrate moisture content, incubation temperature, inoculum si ... | 2013 | 24455400 |