Publications

specificity of the s1 nuclease from aspergillus oryzae.	conditions are described for digesting single-stranded dna by s1 nuclease without introducing breaks in double-stranded dna. the enzyme is inhibited by low concentrations of various compounds of phosphate. under certain conditions s1 nuclease cleaves the strand opposite a nick in bacteriophage t5 dna; under other conditions, the enzyme cleaves a loop in one strand of heteroduplex lambdadna while leaving the opposite strand intact. s1 nuclease makes many single strand breaks in ultraviolet-irradi ...	1975	171268
variations in hybridization of rna from different mouse tissues and embryos to endogenous c-type virus dna transcripts.	several adult tissues, newborns, and embryos of uninfected balb/c mice were analysed for rna complementary to [3h]-dna transcripts synthesized from an endogenous type-c virus of balb/c 3t3. the technique of rna:dna hybridization was used and the extent of hybridization was measured by the use of a single-strand-specific nuclease (s-i), purified from aspergillus oryzae. virus-specific rna was detected in all adult and embryonic tissues tested. however, the rna extracted from tissues having higher ...	1975	169317
enzymatic determination of nonrandom incorporation of 5-bromodeoxyuridine in rat dna.	secondary cultures of normal rat embryo cells were synchronized by a double thymidine block and pulsed with 10(-7) m 5-[3h]bromodeoxyuridine (brdurd) or 10(-7) m[3h]thymidine during an entire s phase (7.5 h). to examine the pattern of [3h]thymidine, dna was immediately extracted and purified at the completion of the s phase, cscl density gradient centrifugation revealed that substitution for thymine by bromouracil was less than 7%. single-strand specific nucleases obtained from aspergillus oryza ...	1976	133713
aflatoxin produced by five species of aspergillus on rice.		1979	114865
[role of metal ions in the catalytic activity of aspergillus oryzae aminopeptidase].	the paper deals with the role of metals in the catalytic action of asp. oryzae aminopeptidase. cobalt ions are more specific activators than mn2+ and mn2+ and evoke its maximal activity. sinergic activation of co2+ in combination with mn2+ and mg2+ was not found in contrast to some aminopeptidases of animal origin. activation of the enzyme with cobalt chloride depends on temperature. by the 10th minute of incubation the activation reaches its maximum: 650 and 900% at 20 and 40 degrees c, respect ...	1979	106499
activation of fungal alpha-amylase by dithioerythritol.	the activity of fungal alpha-amylase has been shown to be influenced by disulfide-reducing reagents. thus, the enzymatic activity increases in the presence of dithioerythritol or 2-mercaptoethanol. l-cysteine is also capable of increasing the activity, but the activation competes with an inactivation reaction which dominates at higher reagent concentrations (greater than 20 mm). a possible scheme interpreting the results is given.	1979	95240
low resolution crystal structures of taka-amylase a and its complexes with inhibitors.	the molecular structure of taka-amylase a, an alpha-amylase from aspergillus oryzae, has been studied at 6 a resolution by x-ray diffraction analysis. the electron density map showed a non-crystallographic three-fold screw arrangement of the molecules in the crystal. the molecule is an ellipsoid with approximate dimensions of 80 x 45 x 35 a and contains a hollow which may correspond to the active center. the inhibitor molecules bind to taka-amylase a at four different sites, one of which is loca ...	1979	93603
mechanism of conjugation and recombination in bacteria. xvii. further evidence for single-stranded regions in recipient dna during conjugation in escherichia coli k-12.	the secondary structure of recipient dna mated with hfr strain was investigated by cscl density gradient fractionation. after 45 min of hfrh64 x 3h-f-ab1157 mating one-fourth of the radioactive recipient dna was recovered as a single-strand but only after shearing of cell lysates prior to centrifugation. this heavier than native dna fraction of radioactive material (obtained after the first centrifugation) was degraded by single-strand specific nuclease s from aspergillus oryzae. these findings ...	1977	67752
histamine induction and release following proteolytic enzyme exposure.	the mechanism of biologic response from exposure to a 12% subtilisin carlsberg preparation is shown to be one of histamine release in the guinea pig. three groups of guinea pigs were pretreated by intradermal injections withsaline solution of (1) the commercial proteolytic enzyme preparation containing 12% subtilisin carlsberg, (2) an alkaline protease preparation obtain from aspergillus oryzae that was isolated from cotton dust, or (3) a nonproteolytic mixture of proteins and lipases obtained f ...	1975	48332
[effect of endonuclease s1 on the dna of normal and tumor cells].		1979	42519
a note on a novel fungal alkaline proteinase inhibitor from aspergillus oryzae.		1979	42393
purification of an alkaline nuclease from physarum polycephalum.	an alkaline nuclease was purified from microplasmodia of physarum polycephalum. the nuclease, active on denatured dna and rna and free of contamination by other nucleolytic activities, appeared to be a zinc-metallo protein. the enzyme was only active under conditions, where zn2+ were retained in the enzyme. loss of zinc occurred by the chelating action of edta, egta or ampholines, by acid of highly alkaline ph conditions or by high ionic strength. the addition of zncl2 to compensate losses, rest ...	1979	41584
[properties of amylase immobilized on aerosil derivatives].	the properties of three preparations of alpha-amylase immobilized on aminoaerosil by 2,4-toluylenediisocyanate and n,n'-dicyclohexylcarbodiimide as well as on carboxyaerosil by n,n'-dicyclohexylcarbodiimide were compared with the properties of soluble enzyme. under immobilization the ph-effect and ph-stability zones of amylase are 0.5--1.0 ph units shifted towards the alkaline region. the preparations in which the enzyme is bound with the matrix through the amine groups on carboxyaerosil by n,n' ...	1979	38550
[immobilization of aspergillus oryzae aminopeptidase on organic and inorganic carriers].	the process of asp. oryzae aminopeptidase immobilization on organic (ae-cellulose, sepharose 4b, sephadex g-200) and inorganic (sch-2, sch-3 sylochromes and kck n 1 silicagel) carriers was studied. aminopeptidase immobilized on sephadex g-200 contains the largest amount of protein (80 mg per 1 g of carrier) and is the most active of all other preparations. the immobilized preparations retain the temperature optimum, like the soluble form, at 60 degrees c, except the preparation immobilized on ae ...	1979	38548
[stability of alpha-amylase with immobilization through its different functional groups].	the paper deals with stability of aspergillus aryzae alpha-amylase immobilized on cm- and ae-celluloses using n,n'-dicyclohexylcarbodiimide by means of binding through its amine or carboxylic groups. the binding of the enzyme with cm-cellulose makes its preparations more stable to the effect of edta and elevated temperature (50 degrees c) than under fixation on ae-cellulose.	1979	36704
single-stranded regions in streptococcus pneumoniae chromosomal deoxyribonucleic acid and their relation to transformation.	deoxyribonucleic acid (dna) in lysates of both completent and noncompetent streptococcus pneumoniae cells was characterized by chromatography on benzoylated, naphthoylated diethylaminoethyl-cellulose columns, by sensitivity to aspergillus oryzae s1 endonuclease, and by sucrose gradient analysis. the dnas from both competent and noncompetent cells were found to contain similar extents of single-stranded regions. these single-stranded regions appeared to be intact, unpaired regions in double-stran ...	1979	35514
comparative studies of three exo-beta-glycosidases of aspergillus oryzae.	beta-glucosidase [beta-d-glucoside glucohydrolase ec 3.2.1.21] and beta-galactosidase [beta-d-galactoside galactohydrolase, ec 3.2.1.23] of takadiastase were purified by acetone fractionation, deae-cellulose, and hydroxylapatite chromatography. purity was confirmed by disc electrophoresis, ultracentrifugation and measurement of other glycosidase activities which coexisted in takadiastase. molecular weight of the beta-glucosidase was 218,000 by sedimentation equilibrium and 110,000-116,000 by sds ...	1979	33973
[acid-stable and acid-unstable alpha-amylases of the mold fungi aspergillus].	acid-sable alpha-amylase of asp. niger and acid-unstable, alpha-amylase of asp. oryzae were studied. it was demonstrated, that beside being a more acid-stable properties, alpha-amylase asp. niger has increased thermal stability as compared to alpha-amylase asp. oryzae. the molecular weights of acid-stable alpha-amylase and acid-unstable alpha-amylase are 58 000 and 51 000, respectively. the amino acid composition, and the c- and n-terminal amino acids of both forms of alpha-amylases were determi ...	1978	31203
presence and partial characterization of internal acid protease of aspergillus oryzae.	the presence and partial characterization of the internal acid protease (ec 2.4.23.6) of aspergillus oryzae has been investigated. although the majority of the acid protease is external and present in the culture filtrate, a significant amount of the active enzyme is firmly bound to the cells; it is not released by repeated extraction of cells with 0.9% sodium chloride but is liberated into the soluble fraction during disruption of cells. the internal acid protease, as well as the external one, ...	1978	29561
how much is secondary structure responsible for resistance of double-stranded rna to pancreatic ribonuclease a?	1. double-stranded f2 sus11 or qbeta rnas, resistant to bovine pancreatic rnaase a in 0.15 m nacl/0.015 m sodium citrate (ssc), are quickly and completely degraded at 10-fold lower ionic strength (0.1 x ssc) under otherwise similar conditions. at this ionic strength the secondary structure of double-stranded rna is maintained, as judged by the following: (a) the unchanged resistance of double-stranded rna and dna, under similar low ionic strength conditions, to nuclease s1 from aspergillus oryza ...	1978	26405
purification and characterization of the two molecular forms of membrane acid protease from aspergillus oryzae.	two forms (m1 and m2) of the membrane-bound acid protease of aspergillus oryzae have been purified by extraction with triton x-100, washing with cold acetone, and repeated gel filtration on bio-gel a-15 m in the presence and absence of triton x-100. the purified membrane enzymes, m1 and m2, moved as a single band in acrylamide gel electrophoresis and had apparent molecular weights of 150 000 and 60 000, respectively, as estimated by sodium dodecyl sulfate/acrylamide gel electrophoresis. these tw ...	1978	25177
immobilization of alpha-amylase on porous glass and silochrome.	immobilized forms of alpha-amylase from aspergillus oryzae were prepared on the porous glass and silochrome by the glutaraldehyde method. an addition of calcium ions at a concentration of 0.05 m to the reaction mixture during immobilization stabilized the enzyme activity. ph optimum of the insoluble form of alpha-amylase was 5.8 and that of the soluble form was 4.7. storage of the insoluble enzyme as water suspension in 0.015 m cacl2 at 4 degrees c for six months and twenty times repeated specif ...	1978	24839
optimal conditions of alpha-amylase production by aspergillus oryzae in liquid media.	the alpha-amylase secretion in a mineral culture medium containing starch and glucose follow the lysis of mycelium. this lysis seems to result from the hydrolysing action of dextranase and levulanase on cell wall. cell lysis and amylase secretion are greatly enhanced by ph elevation of culture medium (optimal ph 8,8). in such conditions of production the amylase is not stable but can be stabilized by addition of starch. a method is described using ph and starch content modifications, which allow ...	1977	23718
isolation and properties of leucine aminopeptidase from aspergillus oryzae.	homogenious leucine aminopeptidase is purified from "oryzine"--mixture of enzymes produced by surface culture of asperigillus oryzae using treatment with activated characoal, followed by deae-cellulose and hydroxylapatite chromatographies, biogel p-100 gel-filtration and polyacrylamide-gel electrophoresis. the enzyme has ph optimum 9.0 and the molecular weight 37500 as estimated by gil-filtration through sephadex g-100 (superfine) and sds-polyacrylamide gel electrophoresis. leucine aminopeptidas ...	1977	19097
preparation and properties of a new dnase from aspergillus oryzae.	a dnase present in commercial preparations of aspergillus oryzae alpha-amylase was purified 1550-fold in 25% yield by acetone precipitation and by chromatography on diethylaminoethyl- and carboxymethylcellulose. the enzyme was isolated free of contaminating rnases and dnases. the molecular weight of the enzyme determined by gel filtration on sephadex g-100 was 48 000, while a molecular weight of 58 000 was determined for the single band observed upon polyacrylamide gel electrophoresis in sodium ...	1977	19042
rapid induction of alpha-amylase by nongrowing mycelia of aspergillus oryzae.	a rapid induction system for synthesis of alpha-amylase by the funga aspergillus oryzae m-13 was established. the mycelia were prepared from 20-h cultures grown on a peptone-glycerol medium and starved for 5 h; maltose was the optimum inducer tested. during h 1 of induction, formation of both intra- and extracellular alpha-amylases occurred at an almost identical rate (70 to 80 microgram/g of cells-h) without a detectable lag period. after a 1-h induction period, a remarkable increase in the ext ...	1977	18989
properties of chymotrypsin proteinase from aspergillus oryzae.	chymotrypsin-type proteinase is detected in the proteolytic system of asp. oryzae. the action of it and chymotrypsin is shown to depend on formaldehyde. hydrolysis of substrates, p-nitrophenyl acetate (p-npa) and n-benzoyl-tyrosine methyl ether (btme), by both preparations is almost the same. the obtained activity ph-optimum for the studied proteinase esterolytic activity is located in the alkaline zone as well as for crystalline chymotrypsin (substrate p-npa). it concerns ph of both enzymes sta ...	1977	18830
gluconic acid forming enzymes in aspergillus niger (author's transl).	at least three gluconic acid forming enzymes were identified in cell-free extracts of aspergillus niger: glucose oxidase (ec 1.1.3.4), a glucose dehydrogenase (ec 1.1.99.10), and an enzyme or a mixture of enzymes catalyzing the cleavage of 6-phosphogluconate into gluconate and inorganic phosphate. 2,6-dichlorphenolindophenol was one of the hydrogen acceptors in vitro of the glucose dehydrogenase. some properties of this enzyme (km values, ph-dependence, substrate and hydrogen acceptor specificit ...	1977	16416
extracellular acid protease of aspergillus oryzae grown on liquid media: multiple forms due to association with heterogeneous polysaccharides.	the acid protease (ec 2.4.23.6) that is produced extracellularly when aspergillus oryzae is grown on liquid media has been isolated and characterized. the enzyme was purified by precipitation with tannic acid, chromatography on duolite a-2, and gel filtration on sephadex g-100. the last step yielded four active components, with varying molecular weights ranging from 42,000 to 60,000. two of them, designated e1 and e1a, with molecular weights of 60,000 and 55,000, respectively, were heterogeneous ...	1977	15987
isolation of amylolytic system of aspergillus oryzae by sorption on deahp amylum.	conditions of effective sorption of amylolytic enzyme from a solution or from fermentative liquid on deahp amylum were studied. isolating action is in a direct dependence on the relation between activity and amount of deahp amylum, the curve of this dependence was illustrated. the enzyme can be released by elution or adsorbate can be used in a pulverised from. in the conclusion of the work laboratory isolation technique is described.	1977	15220
use of amylum derivatives for isolation of amylolytic enzymes.	in a leading article literature is reviewed concerning isolation of amylolytic enzymes by adsorption on differently modified starches, resp. on other adsorbents. deae amylum, deahp amylum and deae-sephadex a 25 were found to be most suitable adsorbents. the other adsorbents examined did not reach claimed parameters.	1977	15219
mechanism of amylase action on glucoside starch bonds.	functional groups of glucoamylase and alpha-amylase from asp. awamori, alpha-amylase from asp. oryzae and alpha- and beta-amylases from barley malt are identified. kinetic curves of the activity dependency on ph, values of ionization heats and photooxidative inactivation draw to the conclusion that carboxyl-imidazole system enters into the active site of the enzymes. a hypothetic mechanism of hydrolysis of alpha-1,4-glucoside bond in starch molecule by alpha- and beta-amylases and of alpha-1,4- ...	1976	14726
characterization of beta-galactosidase from a special strain of aspergillus oryzae.	beta-galactosidase ec 3.2.1.23 was isolated from a partially purified preparation obtained from cultured cells of a special strain of aspergillus oryzae, rt 102 (ferm-p1680). the enzyme preparation gave a single protein band on polyacrylamide gel electrophoresis and was free from alpha-galactosidase, alpha- and beta-mannosidase, alpha- and beta-n-acetylhexosaminidase, and protease activities. the beta-galactosidase was capable of acting on aryl beta-galactosides, lactose, and lactosides. it also ...	1976	14118
evidence for the presence of membrane-bound forms of acid protease in aspergillus oryzae.		1977	13792
purification and characterization of the two molecular forms of aspergillus oryzae acid protease.	the isolation and partial characterization of the acid proteases a1 and a2 (ec3.4.23.6) from aspergillus oryzae grown on solid bran culture are described. the purified preparations were essentially homogeneous by several criteria including sedimentation analysis and polyacrylamide gel electrophoresis. the physiochemical properties of the proteases a1 and a2 were as follows (in the order: a1, a2): molecular weight: 63 000 & 32 000; sedimentation coefficient s20, w: 3.93 and 3.16 s; diffusion cons ...	1976	8138