Publications

renaturation of bacteriophage lambda dna. determination of the optimal renaturation conditions using a single-strand-specific dnase and alkaline-sucrose-gradient assay system.	reannealed hybrid molecules of wild-type bacteriophage lambda dna were prepared in aqueous solutions of formamide at a variety of nacl concentrations at both room temperature ( 22 degrees c) and 37 degrees c. treatment of the hybrid dna molecules with the single-strand-specific nuclease s1 from aspergillus oryzae followed by alkaline sucrose gradient sedimentation was used to monitor the extent and fidelity of hybridization. the optimal renaturation conditions at room temperature were found to b ...	1976	1248479
protein and amino acid changes in peanut (arachis hypogaea l.) seeds infected with aspergillus oryzae.		1976	1245677
chemical mutagenesis. ii. some observations on the new morphogenetic variations induced in aspergillus oryzae wehmer by 6-azauracil.		1975	1242247
studies on the substrate specificity of taka-amylase a. xii. investigation of the active site of taka-amylase a by examining the properties of p-phenylazobenzoyl taka-amylase a.	1. when p-phenylazobenzoyl taka-amylase a (phab-taa) was incubated at ph 6.5 with hydroxylamine for 3 hr at 20degrees, some of the p-phenylazobenzoyl residues that had been introduced into taka-amylase a (taa) [1, 4-alpha-d-glucan glucanohydrolase, ec 3.2.1.1, aspergillus oryzae] were liberated as a hydroxamic acid, and the activity pattern of phab-taa changed to that of intact taa. this result suggested that the p-phenylazobenzoyl residues liberated had been bound to the tyrosyl residue located ...	1975	1225912
[chymotrypsin-like proteinases in microbial proteolytic complexes].	a considerable amount of proteinase of chemotrypsin type (ao-ct) was found in the proteolytic system of asp. oryzae, strain "h"-476. the proteinase produces an intensive effect when hydrolyzing p-nitrophenyl acetate (p-npa) -- 18-20 times as strong as that of crystalline chemotrypsin. comparison of the ao-ct and chemotrypsin properties resulted in proving the enzyme nature of the p-npa hydrolysis reaction. a study of ao-ct specificity with the presence of such substrates as p-npa, o-nitro-phenyl ...	1975	1216345
[effect of iaa on the content of rna in the cells and isolated nuclei of aspergillus oryzae].	the content of rna in mycelium and isolated nuclei of aspergillus oryzae 3-9-15 was studied after its growth during two days on the modified capek medium containing 0.01 and 10 mg% of iaa. a direct correlation has been established between the extracellular concentration of iaa and its content in the mycelium and isolated nuclei of the fungus. the stimulating effect of the extracellular iaa on the content of rna in the isolated nuclei was optimal after one hour of incubation in the capek medium c ...	1975	1214614
[the amino acid sequence of the double-headed protein proteinase inhibitor from dog submandibular glands, i. structural homology to the pancreatic secretory trypsin inhibitors (author's transl)].	the primary structure of the broad specificity proteinase inhibitor from dog submandibular glands was elucidated. the inhibitor consists of a single polypeptide chain of 117 amino acids which is folded into two domains (heads) connected by a peptide of three amino acid residues. both domains i and ii show a clear structural homology to each other as well as to the single-headed pancreatic secretory trypsin inhibitors (kazal type). the trypsin reactive site (-cys-pro-arg-leu-his-glx-pro-ile-cys-) ...	1975	1213678
[denaturation stabilization of alpha-amylase from aspergillus oryzae].	denaturation of alpha-amylase from aspergillus oryzae was studied under the effect of heating urea and some other denaturating agents. inhibition in the enzyme denaturation, deviation from the first order equation and, consequently, establishment of the false equilibrium in the system are shown. the values are calculated for the reaction rate constants of alpha-amylase denaturation under the effect to heat (40 degrees c) and urea. a method is developed for isolating native amylase stabilized by ...	1975	1209773
the atmospheric fungal flora of the athens metropolitan area.	in the research programme of the department of microbiology of the athens university the nature of the mycological flora of the athenian air was studied. the research took place during the calendar year 1971. the open air was sampled twice weekly from two observation stations. the open plate technique was used, petri dishes containing sabourand's agar being exposed for 15 minutes. a total of 180 plates were exposed, and 1714 fungal colonies were isolated; these were subcultured and identified as ...	1975	1207717
studies on the arylsulphatase and phenol sulphotransferase activities of aspergillus oryzae.	1. crude extracts of aspergillus oryzae grown under conditions of sulphur limitation possess high arylsulphatase activity. 2. this activity can be greatly enhanced by the inclusion of tyramine or a number of other phenols in the assay medium. 3. the arylsulphatase activity of these extracts can be resolved into three distinct fractions by chromatography on deae-cellulose. 4. the effect of tyramine is restricted to one of these fractions only. 5. evidence is presented which indicates that this ef ...	1975	1200998
s1 nuclease hydrolysis of single-stranded nucleic acids with partial double-stranded configuration.	the single-strand specific nuclease s1 from aspergillus oryzae (ec 3.1.4.21) was purified 600-fold in 16% yield from dried mycelia. determination of the isoelectric point of s1 nuclease as 4.3-4.4 allowed adjustment of chromatographic conditions such that the enzyme was isolated free of contaminating ribonucleases t1 and t2. s1 nuclease so purified was used for removal of single-stranded portions from the rna of the escherichia coli phage ms2, which has a helical content of about 65% in vitro. a ...	1975	1182098
a chemical and biological assessment of aspergillus oryzae and other filamentous fungi as protein sources for simple stomached animals.		1975	1172167
[growth patterns of fungi].		1975	1169575
purification and some properties of the soluble and membrane-bound adenosine deaminases of micrococcus sodonensis atcc 11880 and their distribution within the family micrococcacea.	in micrococcus sodonensis and some other micrococcus species, adenosien deaminase is present both as a membran-bound and a soluble enzyme; the membran-bound adenosine deaminase can be extracted with n-butanol, and may account for up to 5% of the total cellular adenosine deaminase activity. in a number oc comparative tests, no differences between the two enzyme forms could be found, thus they are believed to be similar molecular species; the purified membran-bound or soluble enzyme had a molecula ...	1975	1168529
in vitro recognition of carcinogen-induced local denaturation sites native dna by s1 endonuclease from aspergillus oryzae.		1975	1161018
triacetonamine formation in fungal extracts.		1975	1159172
[action of leucine aminopeptidase 1 and 2 (aspergillus oryzae 410) on various synthetic peptides (author's transl)].	by use of di- or tripeptides as substrates, lap 1 and lap 2, 2 fractions from aspergillus oryzae hydrolyze oligopeptides that possess leucine as n-terminal amino acid. lap 1 fraction also hydrolyzes the histidyl bond. both fractions have no activity towards peptides as glutathion, gly-pro-ala; they have low or no activity towards tyrosyl and tryptophanyl bond.	1975	1157844
transformation of malathion by a fungus, aspergillus oryzae, isolated from a freshwater pond.		1975	1148416
specific hydrolysis of the cohesive ends of bacteriophage lambda dna by three single strand-specific nucleases.	procedures have been worked out for aspergillus nuclease s1 and mung been nuclease to quantitatively cleave off both of the 12-nucleotide long, single-stranded cohesive ends of lambdadna. this cleavage is indicated by the almost complete elimination of the repair incorporation of radioactive nucleotides by dna polymerase into the digested dna. with s1 nuclease, cleavage was complete at 10 degrees as well as at 30 degrees. under the conditions for quantitative cleavage of the single-stranded regi ...	1975	1141222
reversal of fungitoxicity of 8-quinolinols and their copper (ii) bischelates. i. amino acids and related compounds.	the effect of amino acids and related compounds on the toxicity of 8-quinolinols and their copper (ii) bischelates to aspergillus oryzae (atcc 1011) was studied. none of the compounds tested except the thiol-containing compounds, cysteine, cysteamine, glutathione, and n-acetylcysteine reversed the inhibitory action of 8-quinolinol but not that of 5-iodo-8-quinolinol or bis (8-quinolinolato) copper (ii). it appears that the mechanism(s) of fungitoxicity of 8-quinolinol is different from that of 5 ...	1975	1116048
fatal aspergillosis in imported parrots.	spontaneous fatal aspergillosis occurred in several species of parrots imported from latin america, australia, malaya and ghana for studies on the control of psittacosis. over a period of 4 years, 655 parrots were imported for use in these studies. all birds that died during these investigations were necropsied, and the internal organs of 45 were found to have macroscopic lesions suggestive of aspergillosis. of these 45 suspected cases, 32 were confirmed as aspergillosis by both histopathology a ...	1975	1097931
investigation into the use of aspergillus oryzae s1 nuclease in the presence of solvents which destabilize or prevent dna secondary structure: formaldehyde, formamide, and glyoxal.		1975	1093442
the enhancing influence of proteolysis on e rosette forming lymphocytes (t cells) in vivo and in vitro.	the t lymphocytes populations of 22 young healthy adults, 21 healthy middle aged and older blood donors, 35 non-pregnant women of child bearing age and 14 patients with advanced malignant disease were assessed and compared. it was found that the mean t cell counts in the middle aged and older controls were significantly lower than in the healthy young adults and were further reduced in the patients with malignant disease. the addition of the proteolytic agent brinase (protease 1 obtained from as ...	1975	1080669
transglycosylation by the exo-type of beta-n-acetyglucosaminidase from human parotid saliva.		1975	1057392
comparison of the effects of linear and cyclic phosphates on the adsorption of proteins by human enamel.	treatment of human enamel powder with cyclic trimetaphosphate or linear tripolyphosphate changes subsequent adsorption of amylase, lysozyme, and salivary protein from aqueous solutions. treated enamel samples adsorb more lysozyme, less amylase, and more salivary protein (at concentrations exceeding 12 mug/ml) than controls treated with distilled water.	1975	1056353
thrombolytic treatment with i.v. brinase of advanced arterial obliterative disease of the limbs.	the material includes 17 patients suffering from different degrees of chronic peripheral arterial disease (11 chronic patients stage iii and iv, two patients with acute arterial occlusion, and four patients stage ii). presence and extent of arterial occlusion was ascertained by initial arteriography. in twelve of the patients amputation had been considered. the patients were treated by a series of i.v. infusions of brinase, a proteolytic enzyme from aspergillus oryzae. the brinase inhibitor capa ...	1975	1053595
[hydrolysis of insoluble bone collagen by crystalline alpha-amylase from aspergillus oryzae].	the paper deals with hydrolysis of bone collagen thrice recrystallized alpha-amylase. the enzyme action was estimated by the amount of released glycopeptides, free carbohydrates, alpha-amine groups and total nitrogen in the soluble part of the hydrolyzate. the protease admixture in the alpha-amylase preparation was found by means of the aspergillus oryzae protease. the data obtained testify to the fact that hydrolysis of collagen under the effect of crystalline alpha-amylase occurs only due to t ...	1976	1021920
proteinase inhibitors from cereal grains.		1976	1012027
structure of cerebroside in aspergillus oryzae.	structural studies on cerebroside isolated from aspergillus oryzae were carried out using mainly gas-liquid chromatography-mass spectrometry. the major component fatty acids were 2-hydroxystearic and 2-hydroxy-trans-octadecenoic acid; branched 17?-methyl nonadecasphingadienine isomers were the predominant long-chain bases. the component sugar was only glucose. the structure of the major cerebrosides was assumed to be n-2'-hydroxystearoyl-1-o-glucosyl-17?-methyl sphingadienine and n-2'-hydroxyoct ...	1976	1009131
effect of brinase (protease i from aspergillus oryzae) on the elimination of fibrin from the lungs and pulmonary damage in rats with induced intravascular coagulation and inhibited fibrinolysis.		1976	1006629
conformation of mammalian 5.8 s ribosomal rna: s1 nuclease as a probe.		1976	1001452
subsite mapping of enzymes. application of the depolymerase computer model to two alpha-amylases.	in the preceding paper (allen and thoma, 1976) we developed a depolymerase computer model, which uses a minimization routine to establish a subsite map for a depolymerase. in the present paper we show how the model is applied to experimental data for two alpha-amylases. michaelis parameters and bond-cleavage frequencies for substrates of chain lengths up to twelve glucosyl units have been reported for bacillus amyloliquefaciens, and a subsite map has been proposed for this enzyme [thoma et al. ( ...	1976	999630
aspergillus oryzae (nrrl strain 1988): a clarification.		1976	996551
fermentative production of limit dextrinase by aspergillus oryzae.		1976	992794
[production of immobilized alpha-amylase and its properties].	active immobilized alpha-amylase was obtained when applying ae-cellulose chromatography and glutaric dialdehyde. the time of the enzyme interaction with the carrier, amount of glutaric dialdehyde necessary for binding, optimal enzyme: carrier ratio as well as the methods for desiccation of the immobilized amylase preparations were specified. conditions are selected for alpha-amylase stabilization with the presence of glutaric aldehyde. immobilized amylase as compared to free enzyme is shown to b ...	1976	982618
quantitative analysis of the action of taka-amylase a on maltotriose.	the action of taka-amylase a from asp. oryzae was studied quantitatively by the product analysis method using unlabeled maltotriose and maltotriose labeled at the reducing end as substrates. it was found that the ratio of the unlabeled products, maltose (g2) and glucose (g1) exceeded unity, and that the ratio of the labeled products, g2/g1 was strongly dependent on the initial substrate concentrations. the results can only be explained by a transglycosylation or condensation mechanism or both. a ...	1976	977557
attachment of thioglycosides to proteins: enhancement of liver membrane binding.	thioglycosides of d-galactose, d-glucose, n-acetyl-d-glucosamine, and d-mannose were covalently attached to aspergillus oryzae alpha-amylase, hen's eggs lysozyme, and bovine serum albumin by amidination, diazo coupling, and amide formation. the binding of the newly formed glycoproteins (neoglycoproteins) to rabbit liver membranes was measured, using asialoorosomucoid as a reference. attachment of d-galactosides by any of the three methods enhanced binding by several orders of magnitude. coupling ...	1976	963013
visualization of leucineaminopeptidase activity after acrylamide gel electrophoresis.		1976	962113
[effect of the storage conditions on the viability of fungi].		1976	950926
aspergillus oryzae meningitis.	in a patient with only meningitis, a septate hypha was seen in a langhans giant cell, and the rarely pathogenic aspergillus oryzae was cultured from the cerebrospinal fluid. serologic results confirmed the diagnosis. the patient responded to therapy with amphotericin b and flucytosine.	1976	946540
fungus-fermented soybeans benefit the life cycle of japanese quail (coturnix coturnix japonica).	feeding quail chicks diets containing soybeans fermented with two cultures of aspergilli (a. oryzae n.r.r.l. 451 and a. oryzae n.r.r.l. 506) resulted in significantly superior weight gains (p less than 0.05) through a 4-week growth period and confirmed previous observations made with identical cultures in broiler studies. subsequent hen-day egg production and egg size were changed little by diets containing fermented soybeans. the numerical increases in fertility and hatchability were not signif ...	1976	945574
[isolation and properties of the acid carboxypeptidase of aspergillus oryzae].	caboxypeptidase with ph optimum 4--5 for peptide substrates is isolated from "oryzine", the enzyme mixture produced by aspergillus oryzae, by means of successive salt fractionation, sephadex g-75 gel filtration, chromatography on amberlite irc-50, hydroxylapatite, deae-cellulose and polyacrylamide gel electrophoresis. its molecular weight, as determined by means of polyacrylamide gel electrophoresis in the presence of sodium dolecylsulphate, was found to be 37 000. the enzyme has a broad substra ...	1976	942547
natural plant enzyme inhibitors. v. a trypsin/chymotrypsin inhibitor from alocasia macrorhiza tuber.	a trypsin/chymotrypsin inhibitor was isolated from the tubers of alocasia macrorhiza by extraction at ph 7.6, heat treatment at 80 degrees c, ammonium sulphate precipitation and successive column chromatography on cm-cellulose, deae-sephadex a-50 and sephadex g-100. the inhibitor was pure by cellulose acetate electrophoresis. the molecular weight was approximately 32 000 as determined by gel filtration on sephadex g-100. the inhibitor acted on bovine trypsin, human trypsin and bovine chymotrypsi ...	1977	911861
isolation and amino-terminal sequence analysis of a new pancreatic trypsinogen of the african lungfish protopterus aethiopicus.	the purification and characterization of three pancreatic trypsinogens a1, a2, and a3, from the african lungfish, protopterus aethiopicus, is reported. these zymogens are activated by trypsin, by enterokinase, by an acid protease from aspergillus oryzae, and by autoactivation. the three trypsinogens contain the same amino-terminal amino acid sequence, beginning with the activation peptide leu-pro-leu-glu-asp-asp-lys-. like the activation peptide of the previously characterized trypsinogen b [ree ...	1977	911766
specific hydrolysis of rabbit globin messenger rna by s1 nuclease.	s1 nuclease isolated from aspergillus oryzae has been used to investigate the secondary structure of rabbit globin messenger rna (mrna). the enzyme, which is specific for single stranded nucleotides, digests globin mrna to a limited extent, with 65-75% of the mrna nucleotides resistant to digestion under mild conditions. this limited digestion is not due to enzyme inactivation, but rather to the normal activity of the single-strand nuclease. the reaction was studied as a function of temperature, ...	1977	909777
degradation of nucleic acids in cell lysates by s1 nuclease in the presence of 9 m urea and sodium dodecylsulfate.	single-strand-specific nuclease s1 from aspergillus oryzae is shown to degrade dna and rna in lysates of hela cells in the presence of 9 m urea and sodium dodecylsulfate. free dodecylsulfate inhibits s1 nuclease. however, if the detergent is complexed with proteins prior to the addition of the enzyme, s1 nuclease can degrade nucleic acids at dodecylsulfate concentrations which would inhibit the enzyme completely if no other proteins were present. in lysates prepared from hela cells by treatment ...	1977	908332
s1 nuclease from aspergillus oryzae for the detection of dna damage and repair in the gamma-irradiated intracerebral rat gliosarcoma 9l.		1977	905506
chemical properties of the polysaccharides associated with acid protease of aspergillus oryzae grown on solid bran media.		1977	881411
aspergillus oryzae (nrrl strain 1988)		1977	867035
isolation of amylolytic system of aspergillus oryzae on deae amylum.	studying isolation technique with deae amylum the method was found to be suitable for isolation of alpha-amylase. after determination of optimal conditions sorption curves were illustrated, from which the amount of deae amylum needed for 100% enzyme sorption from solutions of various activity and origin can be calculated. preparation obtained shows high temperature stability and can be used in food industry and agriculture or after its elution in pharmacy. at the end of the paper laboratory isol ...	1977	846561
[peptidases of preparations of proteolytic enzymes from several species of bacteria, molds and actinomycetes].	the activity of carboxy- and aminopeptidases of enzymic preparations obtained from bacteria bacillus subtilis 1 m, 17, bacillus mesentericus 100/26, 100/56, 16; pseudomonas aeruginosa 1/7a, 1/4; bacillus thermophilicus s. sp. nov b-22, moulds aspergillus oryzae 1-746, aspergillus flavus 716 and actinomycete streptomyces griseus 32-13 was studied. in the bacterial preparations the proteolytic activity was 1.5-2.5 times lower than in the fungal and actinomycete preparations. the carboxypeptidase a ...	1976	825850
[degradation of the human igg molecule by a fungal fraction with leucineaminopeptidase activity. preliminary results].		1976	819165
studies on lipids of natto.		1976	815306
amino acid analogs. iii: new syntheses of monomethyl- and monophenylglutamic acids.	glutamic acid analogs containing 3- and 4-methyl and 2-, 3-, and 4-phenyl substituents were prepared. the 3- and 4-methyl- and 3- and 4-phenylglutamic acids did not inhibit plasmodium berghei and were nontoxic to the host (mice) at 640 mg/kg. the five analogs in addition to 2-methlglutamic acid were inactive against lactobacillus casei at 1000 mug/ml in a defined medium: against escherichia coli, only 2-methylglutamic acid caused 27% inhibition at 10,000 mug/ml. all six analogs failed to inhibit ...	1975	811786
the capacity of alpha-amylases to catalyze the nonhydrolytic degradation of starch and glycogen with formation of novel glycosylation products.		1975	810091
properties of limit dextrinase of aspergillus oryzae.		1978	753749
the influence of charged matrix surfaces on the thermostabilizing effect of calcium ions on immobilized fungal alpha-amylase.	the stabilizing effect of calcium ions on fungal alpha-amylase (ec 3.2.1.1) immobilized on a polystyrene anion exchanger (p+ amylase) was investigated and compared to the behaviour of soluble amylase. moreover, gamma-(1,4-benzoquinone-2-yl)-aminopropyl silica-amylase (si(n) amylase) as a conjugate with weakly basic amino groups and gamma-succinamidopropyl silica amylase (si- amylase) as a conjugate with free carboxyl groups were applied for comparison. depending on the calcium ion concentration ...	1978	749472
[role of cyclobutane pyrimidine dimers in uv-denaturation of dna].		1978	739994
on a rabbit hyperlipemia induced by a fungic galactomannane peptide.	i.v. injection into rabbits of a fungic galactomannane peptide isolated from the culture medium of aspergillus oryzae induced the apparition, 20 h later, of an hypertriglyceridemia, with a concomittant decrease of about 70% of the post-heparin lipoprotein lipase activity. the same effect had been obtained earlier with a carbohydrate-rich fraction purified from a crude papain preparation. the 2 fractions are compared.	1978	738437
induction and repair of strand breaks and 3'-hydroxy terminals in the dna of mouse brain following gamma irradiation.	dna was isolated from mouse brain after in vivo gamma-ray irradiation, treated with endonuclease s1 from aspergillus oryzae if necessary, and analysed further by alkaline and neutral sucrose gradient centrifugation. in parallel, its template activity was determined by dna polymerase (ec 2.7.7.7, enzyme a of klenow from escherichia coli) assay as described previously. similar experiments were performed with cultured mouse leukaemia cells (l5178y) irradiated in vitro at 0 degrees c. irradiation in ...	1978	718924
[experiences with substitution therapy using a new pancreatic enzyme of plant origin].	the indication field of nortase, a combination of microbial lipolytic and proteolytic enzymes, comprises the replacement therapy of maldigestion and insufficiency of pancreas. its efficacy and tolerance were tested in 100 patients in an open study under the conditions of general practice. during the 15-day treatment the following symptoms were evaluated: anorexia, flatulence, pressure and pain in the epigastrium, nausea after the meals, belching, pyrosis, the quality of feces and the body weight ...	1978	700583
mapping of deoxydi- and trinucleotides liberated by the action of endonucleases from the silkworm and aspergillis oryzae.	both silkworm nuclease and nuclease o of aspergillus oryzae mycelia hydrolyse dna endolytically to di- and trinucleotides terminating in 5'-phosphate. these oligonucleotides were fractionated first be deae-cellulose chromatography with 7 m urea into the respective isoplithic groups and then analysed for the composition and isomerism: each group was labeled 5'-terminally by the [gamma-32p]atp-polynucleotide kinase reaction and then electrophoresed monodimensionally for the dinucleotides and two-d ...	1978	656423
rate equation for amylase-catalyzed hydrolysis, transglycosylation and condensation of linear oligosaccharides and amylose.		1978	632229
mycotoxins of aspergillus oryzae strains for use in the food industry as starters and enzyme producing molds.		1977	613924
release of peptide-bound sialic acid from landschütz ascites-tumour cells by proteinase 1 of aspergillus oryzae [proceedings].		1977	598591
differential repression of arylsulphatase synthesis in aspergillus oryzae.	1. the activities of the three arylsulphatases (arylsulphate sulphohydrolase, ec 3.1.6.1) of aspergillus oryzae produced under a variety of repressing and non-repressing conditions were determined. 2. these enzymes exhibit different sensitivities to repression by inorganic sulphate. 3. arylsulphatase i, but not arylsulphatases ii and iii, exhibits a transient de-repression in the early growth phase in sulphate media. 4. when the fungus is cultured in repressing media and subsequently transferred ...	1977	597236
a rapid direct assay for the determination of the separate activities of the three arylsulphatases of aspergillus oryzae.	1. the three arylsulphatases of aspergillus oryzae exhibit pronounced kinetic differences and substrate specificities. arylsulphatase i hydrolyses all substrates tested, whereas arylsulphatase iii will not hydrolyse tyrosine o-sulphate or phenolphthalein disulphate. arylsulphatase ii does not hydrolyse p-nitrophenyl sulphate or phenolphthalein disulphate at appreciable rates in the absence of added phenolic compounds. phenols such as tyramine increase the rate of hydrolysis of these substances b ...	1977	597235
kinetic studies of the phenol sulphate-phenol sulphotransferase of aspergillus oryzae.	arylsulphatase ii of aspergillus oryzae exhibits both hydrolytic and sulphotransferase activities. the kinetic data suggest the formation of an intermediate covalent enzyme-sulphate complex with transfer of sulphate from donor to acceptor proceeding via a ping pong mechanism. the unusual kinetic behaviour when 2-hydroxy-5-nitrophenyl sulphate is the substrate is also consistent with this mechanism.	1977	588253
the nutritive value of dehulled soybeans fermented with aspergillus oryzae or rhizopus oligosporus as evaluated by rats.		1979	571903
studies on the inhibitory effects of zinc heptanoate on microorganisms.	inhibitory effect of zinc heptanoate was observed on different cultures of bacteria and fungi. growth of all the bacteria was inhibited by the compound. greatest inhibition was seen in the case of staphylococcus albus, streptococcus pyogenes, shigella dysenteriae, shigella sonnei, shigella flexneri, salmonella typhi, s. paratyphi a, s. paratyphi b, vibrio cholerae, corynebacterium diphtheriae, and e. coli whereas least inhibition was found in the case of staphylococcus aureus. in triethanolamine ...	1979	553835
[isolation and some properties of homogenous nuclease s1 from alpha-amyloryzin].	a purification method for isolating homogeneous single-strand specific nuclease s1 from alpha-amyloryzin has been developed. the yield was about 16% and purification factor--9000. nuclease s1 thus obtained was proved to be free of contaminations of any others nucleolytic enzymes. it is shown for the first time that ribo- and deoxy-dinucleosidemonophosphates are hydrolyzed by nuclease s1 to form 5'-nucleotides with ph optimum for apa equal to 4.6.	1979	547181
[immunological studies on allergic bronchopulmonary aspergillosis--probably induced by aspergillus oryzae (author's transl)].		1979	541917
[3-(1,4-cyclohexadienyl)-l-alanine,8-lysine]vasopressin: synthesis and some pharmacological properties.	[3-(1,4-cyclohexadienyl)-l-alanine,8-lysine]vasopressin, otherwise known as [3-(2,5-dihydrophenylalanine),8-lysine]vasopressin or [dihphe3]lysine-vasopressin, has been synthesized in an attempt to utilize 2,5-dihydrophenylalanine (dihphe) to evaluate the contribution of aromaticity in position 3 to biological activity. the analogue has the same primary structure as lysine-vasopressin, except that two additional hydrogen atoms are present on the ring moiety of the phenylalanine residue in positio ...	1979	536993
[kinetic parameters of superhelical dna cleavage by endonuclease s1].	major kinetic parameters of endonuclease s1 were determined on superhelical bacteriophage pm2 dna and on relaxed nicked circular pm2 dna. at 37 degrees and 0,25 m nacl, the michaelis constants were respectively 1.7 . 10(-8) m and 1 . 10(-9) m, and catalytic constants were respectively 0.36 sec-1 and 1.2 . 10(-2) sec-1. the inhibition of the enzyme reaction by its product was detected.	1979	503058
differential excision from dna of the c-8 deoxyguanosine reaction products of n-hydroxy-2-aminofluorene and n-acetoxy-n-acetyl-2-aminofluorene by endonuclease s1 from aspergillus oryzae.	calf thymus dna was modified in vitro with [g-3h]n-hydroxy-2-aminofluorene and [g-3h]n-acetoxy-n-acetyl-2-aminofluorene and the nuclease s1 digestion was studied under identical conditions. the ratios of the maximum reaction rate (v) and the michaelis constant (km), v/km, indicate that 2-aminofluorene(af)-modified dna is hydrolyzed 3 times more slowly than n-acetyl-2-aminofluorene(aaf)-modified dna under similar reaction conditions. the af-modified dna was slightly more susceptible to partial di ...	1979	476609
[nuclease from aspergillus oryzae, specific for single-stranded regions of nucleic acids].	a single-stranded specific nuclease has been purified from amyloryzine obtained from the mould fungi aspergillus cryzae. the nuclease under study resembles the enzymes described in the literature in its ability to hydrolyze single-stranded nucleic acids. however, the enzyme essentially differs from previously known nucleases in some catalytic properties, particularly in its ability for degradation of poly a. it has been shown that the enzyme also hydrolyzes the synthetic dinucleotide ptpt to mon ...	1979	465607
[formation of volutin inclusions in the mycelium of aspergilli growing on media with hydrocarbons].		1979	440149
simple and rapid colorimetric method for the microdetermination of alpha amylase.		1977	412348
studies on the restoration of the activities of ribonucleases by polyamines in the presence of various ribonuclease inhibitors.	the effect of polyamines on ribonucleases in the presence of various inhibitors (poly(g), heparin, and rat liver rnase inhibitor) has been studied. bovine pancreatic rnas a and a ribonuclease from horse submaxillary gland (rnase hs) were inhibited by the inhibitors, but rnase t1 and rnase m were not inhibited. polyamines were found to restore the activites of rnase a and rnase hs inhibited by poly(g) or heparin but not those activities inhibited by rat liver rnase inhibitor. when poly(u) and pol ...	1977	405377
[drug treatment of meteorism. results of a double blind test].		1977	333264
release of escherichia coli dna from membrane complexes by single-strand endonucleases.	treatment of gently prepared lysates of escherichia coli with single-strand-specific endonuclease (si or from mung beans) results in the release of about 90% of the dna from membranes, as determined by the m band technique. the released dna has an average molecular weight of about 1.2 x 10(8). data obtained with endonuclease s1 fit a mathematical model in which substrate sites are at or near membrane attachment sites. data obtained with pancreatic deoxyribonuclease or x-rays fit a model for doub ...	1977	331316
the predominantly nonhydrolytic action of alpha amylases on alpha-maltosyl fluoride.	crystalline alpha amylases from a number of sources utilized alpha-maltosyl fluoride as a glycosyl donor and acceptor at high rates (approximately 10 to approximately 1550 mumol/min/mg of protein, for 30 mm substrate). all enzymes catalyzed conversion of this compound into maltooligosaccharides in preference to causing its hydrolysis. maltotetraosyl flouride and maltooligosaccharides of d.p. 3 to 6+ accounted for 75--93% (by weight) of early reaction-products. at a late stage, the yield of malto ...	1979	313247
[production of mutants with an increased alpha-amylase synthesis].	a mutant characterized by elevated biosynthesis of alpha-amylase was obtained as a result of a three-stage induced selection using nitroso compounds. changes of mutagens in the course of selection stages and the establishment of their effective doses causing the maximum accumulation of mutations yielded the mutant which produced 2.5 times more alpha-amylase than the parent strain of aspergillus oryzae 762. the induced variability of the mutant can be registered on a solid growth medium and provi ...	1978	309059
a study of the mechanism of action of taka-amylase a1 on linear oligosaccharides by product analysis and computer simulation.	the action pattern and mechanism of the taka-amylase a-catalyzed reaction were studied quantitatively and kinetically by product analysis, using a series of maltooligosaccharides from maltotriose (g3) to maltoheptaose (g7) labeled at the reducing end with 14c-glucose. a marked concentration dependency of the product distribution from the end-labeled oligosaccharides was found, especially with g3 and g4 as substrates. the relative cleavage frequency at the first glycosidic bond counting from the ...	1978	308947
model for carbohydrase action. aspergillus oryzae alpha-amylase degradation of maltotriose.	aspergillus oryzae alpha-amylase catalyzes degradation of oligosaccharides by a variety of pathways. we present here a quantitative study of the degradation of maltotriose by this amylase. our results lead to a scheme involving multiple transglycosylation reactions and shifted binding due to simultaneous binding of two substrate molecules. the scheme is able to account for the diverse body of information collected for the enzyme. the effect of substrate concentration on the products of maltotrio ...	1978	307964
multimolecular substrate reactions catalyzed by caabohydrases. aspergillus oryzae alpha-amylase degradation of maltooligosaccharides.	aspergillus oryzae alpha-amylase degrades maltooligosaccharides by other pathways besides simple glycosidic bond scission. the utilization of the alternate pathways increases with the concentration of substrate implicating a multimolecular substrate mechanism. reducing-end labeled and uniformly labeled maltooligosaccharides were used to elucidate these alternate degradation mechanisms. condensation followed by hydrolysis is not a significant pathway. transglycosylation is concluded to occur, but ...	1978	307963
studies on the substrate specificity of taka-amylase a. xiii. preparation of 6-deoxy-6-iodomaltooligosaccharides and their inhibitory action against taka-amylase a1.	o-alpha-d-glucopyranosyl-(1 leads to 4)-o-6-deoxy-6-iodo-alpha-d-glucopyranosyl-(1 leads to 4)-d-glucopyranose (6'-mt), o-alpha-d-glucopyranosyl-(1 leads to 4)-6-deoxy-6-iodo-d-glucopyranose (6-m), and o-6-deoxy-6-iodo-alpha-d-glucopyranosyl-(1 leads to 4)-d-glucopyranose (6'-m) were prepared and their inhibitory action against taka-amylase a [ec 3.2.1.1, alpha-1, 4-glucan 4-glucanohydrolase, aspergillus oryzae] was investigated. the inhibitor constants of 6'-mt and 6'-m were 10 mm and 54 mm, re ...	1978	306997
difference spectroscopic study of the interaction between taka-amylase a and substrates.		1978	306996
[alpha-amylase activity in lysosomes of aspergillus oryzae (author's transl)].	the alpha-amylase of mycelial cells of aspergillus oryzae exists in a particular form in 8000 g pellet. the lysosomal localization of acid phosphatase is confirmed by electron microscopy. the purification of lysosomes by discontinuous gradient of sucrose in d2o shows that alpha-amylase activity is bound to these particles.	1978	306932
repetitive attack by aspergillus oryzae alpha amylase.	the action of aspergillus oryzae alpha amylase on reducing-end, and uniformly radiolabeled maltotriose through maltodecaose has been studied. the enzyme is found to hydrolyze more than a single glycosidic bond during enzyme-substrate encounters. the extent of this repetitive attack is quantitated.	1978	306286
characterization of dna used to assay sera for anti-dna antibodies; determination of the specificities of anti-dna antibodies in sle and non-sle rheumatic disease states.	commercial 14c-labeled kb cell dna, widely used to assay sera for anti-dna antibodies, was chromatographed on benzoylated-naphthoylated-deae-cellulose (bndc) and on hydroxyapatite (hap). on bndc, only 25% of the 14c label eluted with 1 m nac1 (kb fraction i) characteristic of ds-dna. fifty-five percent of the label eluted with 50% formamide-1 m nac1 (kb fraction ii) characteristic of ss or denatured dna. on hap, however, none of the 14c label eluted with 0.2 m phosphate buffer as anticipated for ...	1977	300090
purification and characterization of extracellular proteinases of aspergillus oryzae.	the extracellular proteinases of aspergillus oryzae ei 212 were separated into two active fractions by (nh4)2so4 and ethanol fractionation followed by diethylaminoethyl-sephadex a-50 and hydroxyapatite chromatography. the molecular weight was estimated by gel filtration to be about 70,000 and 35,000 for proteinases i and ii, respectively. optimum ph for casein and hemoglobin hydrolysis was 6.5 at 60 c for proteinase i and 10.0 at 45 c for proteinase ii, and for gelatin hydrolysis it was 6.5 at 4 ...	1975	242254
activity of endonuclease s1 in denaturing solvents: dimethysulfoxide, dimethylformamide, formamide and formaldehyde.		1975	241350
on the physical properties and mechanism of action of arylsulfate sulfohydrolase ii from aspergillus oryzae.		1975	241293
[studies on the reaction mechanism of a ribonuclease ii from aspergillus oryzae (author's transl)].	ribonuclease t2 was isolated from an aspergillus oryzae extract. in order to define the substrate specificity, the hydrolysis of a series of 2',3'-cyclic nucleotides was measured semiquantitatively. modifications in all positions of the bases are tolerated, as long as the base stays in the anti conformation or has a chance to return to it; bulky substituents at n-3 of the pyrimidine base lower the rate. so far the conclusion seems justified that the enzyme does not react with the substrates by s ...	1975	240766
aspergillus oryzae acid proteinase. purification and properties, and formation of pi-chymotrypsin.	an acid proteinase from aspergillus oryzae was isolated from a commercial powder by successive (nh4)2so4 fractionation, acetone precipitation, and ion-exchange chromatography on phosphate- and deae-cellulose columns. the purified enzyme was found to be homogeneous by ultracentrifuge-sedimentation analysis (s20, w equal 3.63s), but electrofocusing in polyacrylamide gels and electrophoresis at ph 3.2 revealed that it consists of two very closely migrating bands. no difference in the amino acid com ...	1975	239702
purification and properties of beta-galactosidase from aspergillus oryzae.	beta-galactosidase [ec 3.2.1.23] has been purified from a culture of aspergillus oryzae by 2-propanol fractionation, column chromatography on deae-sephadex a-50 and sephadex g-200. the preparation was homogeneous on ultracentrifugation and disc electrophoresis. the enzyme showed ph optima of 4.5 with onpg-1 as a substrate and 4.8 with lactose as a substrate. the stable ph range was from 4.0 to 9.0 and the optimum temperature was 46 degrees. the michaelis constants were 7.2 x 10-minus 4 m with on ...	1975	236999
specific site of action for single-strand specific nuclease on the double-stranded circular dna intermediates of an avian rna tumor virus.	circular viral dna intermediates obtained from the quail tumor line, qt6, at 1 day after infection, were opened at one specific location by the single-strand specific nuclease, s1, of aspergillus oryzae. this site was no longer accessible to the s1 nuclease when circles were first opened at another location with a restriction endonuclease.	1978	215777
inside 45s ribonucleic acid.		1977	202281
studies on the respiratory system of aspergillus oryzae. v. some properties of the respiratory system of mitochondria from mycelia grown in the presence of chloramphenicol.	presence of chloramphenicol in the growth medium for mycelia of aspergillus oryzae was without effect on the oxidative activity, respiratory control, or p/o ratio of isolated mitochondria. the mitochondria oxidized krebs cycle intermediates even in the presence of cyanide at the concentration markedly inhibiting the normal mitochondrial oxidation. however, the p/o ratio during the mitochondrial oxidation decreased by about 1.0 on addition of cyanide. the c-type cytochromes, shown to occur in lar ...	1977	199770