Publications

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fatal aspergillosis in imported parrots.spontaneous fatal aspergillosis occurred in several species of parrots imported from latin america, australia, malaya and ghana for studies on the control of psittacosis. over a period of 4 years, 655 parrots were imported for use in these studies. all birds that died during these investigations were necropsied, and the internal organs of 45 were found to have macroscopic lesions suggestive of aspergillosis. of these 45 suspected cases, 32 were confirmed as aspergillosis by both histopathology a ...19751097931
[effect of iaa on the content of rna in the cells and isolated nuclei of aspergillus oryzae].the content of rna in mycelium and isolated nuclei of aspergillus oryzae 3-9-15 was studied after its growth during two days on the modified capek medium containing 0.01 and 10 mg% of iaa. a direct correlation has been established between the extracellular concentration of iaa and its content in the mycelium and isolated nuclei of the fungus. the stimulating effect of the extracellular iaa on the content of rna in the isolated nuclei was optimal after one hour of incubation in the capek medium c ...19751214614
purification and some properties of the soluble and membrane-bound adenosine deaminases of micrococcus sodonensis atcc 11880 and their distribution within the family micrococcacea.in micrococcus sodonensis and some other micrococcus species, adenosien deaminase is present both as a membran-bound and a soluble enzyme; the membran-bound adenosine deaminase can be extracted with n-butanol, and may account for up to 5% of the total cellular adenosine deaminase activity. in a number oc comparative tests, no differences between the two enzyme forms could be found, thus they are believed to be similar molecular species; the purified membran-bound or soluble enzyme had a molecula ...19751168529
[growth patterns of fungi]. 19751169575
a chemical and biological assessment of aspergillus oryzae and other filamentous fungi as protein sources for simple stomached animals. 19751172167
transformation of malathion by a fungus, aspergillus oryzae, isolated from a freshwater pond. 19751148416
[action of leucine aminopeptidase 1 and 2 (aspergillus oryzae 410) on various synthetic peptides (author's transl)].by use of di- or tripeptides as substrates, lap 1 and lap 2, 2 fractions from aspergillus oryzae hydrolyze oligopeptides that possess leucine as n-terminal amino acid. lap 1 fraction also hydrolyzes the histidyl bond. both fractions have no activity towards peptides as glutathion, gly-pro-ala; they have low or no activity towards tyrosyl and tryptophanyl bond.19751157844
triacetonamine formation in fungal extracts. 19751159172
studies on the arylsulphatase and phenol sulphotransferase activities of aspergillus oryzae.1. crude extracts of aspergillus oryzae grown under conditions of sulphur limitation possess high arylsulphatase activity. 2. this activity can be greatly enhanced by the inclusion of tyramine or a number of other phenols in the assay medium. 3. the arylsulphatase activity of these extracts can be resolved into three distinct fractions by chromatography on deae-cellulose. 4. the effect of tyramine is restricted to one of these fractions only. 5. evidence is presented which indicates that this ef ...19751200998
the atmospheric fungal flora of the athens metropolitan area.in the research programme of the department of microbiology of the athens university the nature of the mycological flora of the athenian air was studied. the research took place during the calendar year 1971. the open air was sampled twice weekly from two observation stations. the open plate technique was used, petri dishes containing sabourand's agar being exposed for 15 minutes. a total of 180 plates were exposed, and 1714 fungal colonies were isolated; these were subcultured and identified as ...19751207717
[denaturation stabilization of alpha-amylase from aspergillus oryzae].denaturation of alpha-amylase from aspergillus oryzae was studied under the effect of heating urea and some other denaturating agents. inhibition in the enzyme denaturation, deviation from the first order equation and, consequently, establishment of the false equilibrium in the system are shown. the values are calculated for the reaction rate constants of alpha-amylase denaturation under the effect to heat (40 degrees c) and urea. a method is developed for isolating native amylase stabilized by ...19751209773
[the amino acid sequence of the double-headed protein proteinase inhibitor from dog submandibular glands, i. structural homology to the pancreatic secretory trypsin inhibitors (author's transl)].the primary structure of the broad specificity proteinase inhibitor from dog submandibular glands was elucidated. the inhibitor consists of a single polypeptide chain of 117 amino acids which is folded into two domains (heads) connected by a peptide of three amino acid residues. both domains i and ii show a clear structural homology to each other as well as to the single-headed pancreatic secretory trypsin inhibitors (kazal type). the trypsin reactive site (-cys-pro-arg-leu-his-glx-pro-ile-cys-) ...19751213678
[chymotrypsin-like proteinases in microbial proteolytic complexes].a considerable amount of proteinase of chemotrypsin type (ao-ct) was found in the proteolytic system of asp. oryzae, strain "h"-476. the proteinase produces an intensive effect when hydrolyzing p-nitrophenyl acetate (p-npa) -- 18-20 times as strong as that of crystalline chemotrypsin. comparison of the ao-ct and chemotrypsin properties resulted in proving the enzyme nature of the p-npa hydrolysis reaction. a study of ao-ct specificity with the presence of such substrates as p-npa, o-nitro-phenyl ...19751216345
in vitro recognition of carcinogen-induced local denaturation sites native dna by s1 endonuclease from aspergillus oryzae. 19751161018
studies on the substrate specificity of taka-amylase a. xii. investigation of the active site of taka-amylase a by examining the properties of p-phenylazobenzoyl taka-amylase a.1. when p-phenylazobenzoyl taka-amylase a (phab-taa) was incubated at ph 6.5 with hydroxylamine for 3 hr at 20degrees, some of the p-phenylazobenzoyl residues that had been introduced into taka-amylase a (taa) [1, 4-alpha-d-glucan glucanohydrolase, ec 3.2.1.1, aspergillus oryzae] were liberated as a hydroxamic acid, and the activity pattern of phab-taa changed to that of intact taa. this result suggested that the p-phenylazobenzoyl residues liberated had been bound to the tyrosyl residue located ...19751225912
chemical mutagenesis. ii. some observations on the new morphogenetic variations induced in aspergillus oryzae wehmer by 6-azauracil. 19751242247
s1 nuclease hydrolysis of single-stranded nucleic acids with partial double-stranded configuration.the single-strand specific nuclease s1 from aspergillus oryzae (ec 3.1.4.21) was purified 600-fold in 16% yield from dried mycelia. determination of the isoelectric point of s1 nuclease as 4.3-4.4 allowed adjustment of chromatographic conditions such that the enzyme was isolated free of contaminating ribonucleases t1 and t2. s1 nuclease so purified was used for removal of single-stranded portions from the rna of the escherichia coli phage ms2, which has a helical content of about 65% in vitro. a ...19751182098
purification and characterization of extracellular proteinases of aspergillus oryzae.the extracellular proteinases of aspergillus oryzae ei 212 were separated into two active fractions by (nh4)2so4 and ethanol fractionation followed by diethylaminoethyl-sephadex a-50 and hydroxyapatite chromatography. the molecular weight was estimated by gel filtration to be about 70,000 and 35,000 for proteinases i and ii, respectively. optimum ph for casein and hemoglobin hydrolysis was 6.5 at 60 c for proteinase i and 10.0 at 45 c for proteinase ii, and for gelatin hydrolysis it was 6.5 at 4 ...1975242254
variations in hybridization of rna from different mouse tissues and embryos to endogenous c-type virus dna transcripts.several adult tissues, newborns, and embryos of uninfected balb/c mice were analysed for rna complementary to [3h]-dna transcripts synthesized from an endogenous type-c virus of balb/c 3t3. the technique of rna:dna hybridization was used and the extent of hybridization was measured by the use of a single-strand-specific nuclease (s-i), purified from aspergillus oryzae. virus-specific rna was detected in all adult and embryonic tissues tested. however, the rna extracted from tissues having higher ...1975169317
histamine induction and release following proteolytic enzyme exposure.the mechanism of biologic response from exposure to a 12% subtilisin carlsberg preparation is shown to be one of histamine release in the guinea pig. three groups of guinea pigs were pretreated by intradermal injections withsaline solution of (1) the commercial proteolytic enzyme preparation containing 12% subtilisin carlsberg, (2) an alkaline protease preparation obtain from aspergillus oryzae that was isolated from cotton dust, or (3) a nonproteolytic mixture of proteins and lipases obtained f ...197548332
on the physical properties and mechanism of action of arylsulfate sulfohydrolase ii from aspergillus oryzae. 1975241293
purification and properties of beta-galactosidase from aspergillus oryzae.beta-galactosidase [ec 3.2.1.23] has been purified from a culture of aspergillus oryzae by 2-propanol fractionation, column chromatography on deae-sephadex a-50 and sephadex g-200. the preparation was homogeneous on ultracentrifugation and disc electrophoresis. the enzyme showed ph optima of 4.5 with onpg-1 as a substrate and 4.8 with lactose as a substrate. the stable ph range was from 4.0 to 9.0 and the optimum temperature was 46 degrees. the michaelis constants were 7.2 x 10-minus 4 m with on ...1975236999
aspergillus oryzae acid proteinase. purification and properties, and formation of pi-chymotrypsin.an acid proteinase from aspergillus oryzae was isolated from a commercial powder by successive (nh4)2so4 fractionation, acetone precipitation, and ion-exchange chromatography on phosphate- and deae-cellulose columns. the purified enzyme was found to be homogeneous by ultracentrifuge-sedimentation analysis (s20, w equal 3.63s), but electrofocusing in polyacrylamide gels and electrophoresis at ph 3.2 revealed that it consists of two very closely migrating bands. no difference in the amino acid com ...1975239702
[studies on the reaction mechanism of a ribonuclease ii from aspergillus oryzae (author's transl)].ribonuclease t2 was isolated from an aspergillus oryzae extract. in order to define the substrate specificity, the hydrolysis of a series of 2',3'-cyclic nucleotides was measured semiquantitatively. modifications in all positions of the bases are tolerated, as long as the base stays in the anti conformation or has a chance to return to it; bulky substituents at n-3 of the pyrimidine base lower the rate. so far the conclusion seems justified that the enzyme does not react with the substrates by s ...1975240766
activity of endonuclease s1 in denaturing solvents: dimethysulfoxide, dimethylformamide, formamide and formaldehyde. 1975241350
specificity of the s1 nuclease from aspergillus oryzae.conditions are described for digesting single-stranded dna by s1 nuclease without introducing breaks in double-stranded dna. the enzyme is inhibited by low concentrations of various compounds of phosphate. under certain conditions s1 nuclease cleaves the strand opposite a nick in bacteriophage t5 dna; under other conditions, the enzyme cleaves a loop in one strand of heteroduplex lambdadna while leaving the opposite strand intact. s1 nuclease makes many single strand breaks in ultraviolet-irradi ...1975171268
the capacity of alpha-amylases to catalyze the nonhydrolytic degradation of starch and glycogen with formation of novel glycosylation products. 1975810091
amino acid analogs. iii: new syntheses of monomethyl- and monophenylglutamic acids.glutamic acid analogs containing 3- and 4-methyl and 2-, 3-, and 4-phenyl substituents were prepared. the 3- and 4-methyl- and 3- and 4-phenylglutamic acids did not inhibit plasmodium berghei and were nontoxic to the host (mice) at 640 mg/kg. the five analogs in addition to 2-methlglutamic acid were inactive against lactobacillus casei at 1000 mug/ml in a defined medium: against escherichia coli, only 2-methylglutamic acid caused 27% inhibition at 10,000 mug/ml. all six analogs failed to inhibit ...1975811786
on the pharmacology of brinases, a proteolytic enzyme from aspergillus oryzae. 19744134257
clinical review on brinase, a protease from aspergillus oryzae. 19744134258
[demonstration of extracellular leucine aminopeptidases (ec 3.4.1) of aspergillus oryzae (ip 410). study of the leucine aminopeptidase 2 fraction]. 19744212604
[products of proteolysis obtained with the use of complex enzyme preparations at different ph values]. 19744219043
[extracellular leucine aminopeptidases (ec 3.4.1.1) of aspergillus oryzae (ip 410). glycoprotein nature of the fraction leucine aminopeptidase 1]. 19744219346
[experimental and clinical studies on a protease from aspergillus oryzae (brinase): fibrinogen turnover, thrombocyte aggregation and occlusion of the scribner shunt]. 19744134482
review of the biochemistry and coagulation physiology of brinolase. (fibrinolytic enzyme from aspergillus oryzae). 19744134481
amino acid transport in spores of aspergillus oryzae. 19744436276
[biosynthesis of beta-fructofuranosidase by fungi of the genus aspergillus]. 19744463361
[free intracellular amino acids of aspergillus oryzae mycelia and acid proteinase synthesis]. 19744464877
further evidence on differentiation of aspergillus sojae from aspergillus oryzae by electrophoretic patterns of cellulase, pectin-lyase, and acid proteinase. 19744822056
the formation, general characteristics and production of alpha-amylase in heterocaryons and diploids of aspergillus oryzae. 19744436159
[isolation of fractions having aminopeptidase activity (ec 3.4.1.1) (arylamidase) from culture medium of aspergillus oryzae]. 19744447798
[the effect of sodium chloride and sulfate on the growth and amylolytic activity of the fungus aspergillus oryzae]. 19744449783
single-stranded regions in dna isolated from different developmental stages of the sea urchin.long-term labeled sea urchin embryo (strongylocentrotus purpuratus) dnas were examined for size of recovered pieces, single-strandedness, and length of continuous double-stranded regions. sizing on neutral sucrose gradients indicates that morula stage dna sediments predominantly at 31 s, blastula stage dna at 27 s, and gastrula stage dna as a broad range of sizes of greater than 29 s. treatment of [3h]thymidine-labeled dna with aspergillus oryzae s1 nuclease removes 19% of the 3h from morula sta ...197411400427
isolation of a short, cytosine-rich repeating unit from the dna of escherichia coli.hybridization of heterologous nucleic acids has provided the means for isolating a repeating sequence which is located next to template regions of dna. separated single strands of 32p-labelled dna from escherichia coli were to a limited extent able to anneal with dna of micrococcus lysodeikticus immobilized on nitrocellulose membrane filters. the resulting hybrid was resistant to enzymes specific for unpaired strands, nuclease s1 (aspergillus oryzae) and exonuclease i (e. coli). the e. coli dna ...197411400431
studies on a pyrophosphatase and glucose-6-phosphatase from aspergillus oryzae. 19734358816
factors affecting the synthesis and release of the extracellular protease of aspergillus oryzae nrrl 2160. 19734351078
action of the s1 endonuclease from aspergillus oryzae on simian virus 40 supercoiled component i dna. 19734356815
purification and properties of an isoenzyme of arylsulphatase from aspergillus oryzae. 19734685274
isolation of alkaline proteinases from aspergillus oryzae by one-step affinity chromatography on ovoinhibitor-sepharose column. 19734736151
growth and fat formation of aspergillus oryzae and aspergillus terreus on enzyme-hydrolyzed sweet potatoes. 19734763732
stimulation of induction of glucose dehydrogenase by homologous series of aliphatic alcohols in dormant spores of aspergillus oryzae. 19734729444
self-inhibiting extracellular proteins from aspergillus oryzae. 19734744881
[comparative hygienic evaluation of the complex enzyme preparations obtained from the fungus aspergillus oryzae]. 19734793429
studies on n-acetyl- -d-glucosaminidase of aspergillus oryzae. v. demonstration of an exo-type of the enzyme and determination of partial binding energy of the es-complex. 19734690234
purification and further properties of single-strand-specific nuclease from aspergillus oryzae. 19734691350
[effect of exogenous urea on the amylolytic and proteolytic activities of the fungus aspergillus oryzae]. 19734701626
[localization of the effect of regulation of alpha-amylase biosynthesis by the level of active enzyme in the medium with aspergillus oryzae]. 19734704556
induction of enzymes in dormant spores of aspergillus oryzae. iv. effects of ethidium, aminoacridines, and related compounds. 19734708042
[effect of the chemical structure of polymers on the activity of certain enzymes synthesized by cultures of aspergillus oryzae and bacillus mesentericus]. 19734600723
[selection of producers of amylolytic enzymes]. 19734805933
use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes.bacterial and plasmid homo- and heteroduplexes have been analyzed with a single-strand specific endonuclease, s1, of aspergillus oryzae. under appropriate assay conditions, there was a high degree of correlation between the degree of deoxyribonucleic acid (dna)-dna homoduplex formation assessed by the s1 endonuclease and by hydroxyapatite (ha). heteroduplexes which contain extensive regions of polynucleotide sequences in common are similarly recognized by the s1 endonuclease and ha. in instances ...19734728274
fiber digestion in lambs fed an extract of aspergillus oryzae. 19734797042
microbial formation of nitrosamines in vitro.mortierella parvispora and an unidentified bacterium converted trimethylamine to dimethylamine, and the bacterium (but not the fungus) formed dimethylnitrosamine in the presence of nitrite. dimethylnitrosamine also appeared in cell suspensions of escherichia coli and streptococcus epidermidis and in hyphal mats of aspergillus oryzae incubated with dimethylamine and nitrate. suspensions of a number of microorganisms produced n-nitrosodiphenylamine from diphenylamine and nitrite at ph 7.5, and sol ...19734577486
cleavage of circular, superhelical simian virus 40 dna to a linear duplex by s1 nuclease.s(1) nuclease, the single-strand specific nuclease from aspergillus oryzae can cleave both strands of circular covalently closed, superhelical simian virus 40 (sv40) dna to generate unit length linear duplex molecules with intact single strands. but circular, covalently closed, nonsuperhelical dna, as well as linear duplex molecules, are relatively resistant to attack by the enzyme. these findings indicate that unpaired or weakly hydrogen-bonded regions, sensitive to the single strand-specific n ...19734357509
partial transcription of murine type c viral genomes in balb c cell lines.the mouse cell line, balb/c 3t3, and its derivatives transformed either spontaneously or by treatment with a variety of external agents, were analyzed for cytoplasmic rna complementary to dna products prepared from the kirsten strain of murine sarcoma-leukemia virus, and from an endogenous type c virus of balb/c 3t3. although none of these cell lines spontaneously releases complete type c virions, they all contain rna which is partially homologous to a portion of the 35s rna isolated from these ...19734359949
the purification and properties of an extracellular protease from aspergillus oryzae nrrl 2160. 19734197025
alpha-d-fucosidase from aspergillus oryzae: characterization of alpha-d-fucosidase with alpha-d-galactosidase activity. 19734198930
specificity of the dna product of rna-dependent dna polymerase in type c viruses. ii. quantitative analysis.a number of mammalian type c viruses were analyzed for relatedness by the technique of dna.rna hybridization. viral dnas were prepared in single-stranded form from complexes with 70s viral rna formed during endogenous polymerase reactions. extent of hybridization was assayed with the single-strand nuclease (s-1) from aspergillus oryzae. results obtained indicated a high degree of viral specificity, with significant cross-reactions being observed only with viruses obtained from within a species, ...19734129928
studies on the respiratory system of aspergillus oryzae. iv. low temperature difference spectra of mitochondria from mycelia grown in the presence and absence of chloramphenicol. 19724335785
studies on n-acetyl- -d-glucosaminidase of aspergillus oryzae. 3. transglycosylation by the enzyme and preparation of crystalline phenyl n, n'-diacetyl- -chitobioside. 19724346265
differentiation of aspergillus sojae from aspergillus oryzae by polyacrylamide gel disc electrophoresis. 19725039255
the effects of brinolase (fibrinolytic enzyme from aspergillus oryzae) on platelet aggregation of dog and man. 19725074991
induced morphogenetic variations in aspergillus oryzae by 8-azaguanine. 19724654227
induction of enzymes in dormant spores of aspergillus oryzae. 3. effects of kanamycin and streptomycin. 19724639285
studies on n-acetyl-beta-d-glucosaminidase of aspergillus oryzae. iv. acceptor specifity and quantitative representation of the transglycosylation reaction. 19724664739
[mechanism of the effect of starch on the biosynthesis of alpha-amylase by aspergillus oryzae]. 19724670464
studies on n-acetyl- -d-glucosaminidase of aspergillus oryzae. ii. substrate specificity of the enzyme. 19725015362
alkaline protease from aspergillus oryzae: esterase activity. 19725018277
[role of the cell membrane in regulating the biosynthesis of alpha-amylase in aspergillus oryzae]. 19725037362
thrombolytic properties and side effects of brinase (fibrinolytic enzyme from aspergillus oryzae) in the dog. 19725070267
minimum number of proteases occurring in culture media of aspergillus oryzae. 19715098345
reaction of protein disulfide groups with ellman's reagent: a case study of the number of sulfhydryl and disulfide groups in aspergillus oryzae -amylase, papain, and lysozyme. 19715114933
purification of ribonuclease t 1 by diethylaminoethylcellulose chromatography.a procedure is described for isolating the enzyme ribonuclease t(1) from takadiastase, an extract of the mould aspergillus oryzae. it involves an initial concentration of the enzyme by adsorption on deae-cellulose followed by gradient elution. later the enzyme is chromatographed on the same adsorbent with an eluent of constant composition. yields of 350-380mg of ribonuclease t(1) from 500g of takadiastase were obtained.19715114970
the effects of protease i of aspergillus oryzae (brinase) on membrane permeability and growth of landschütz ascites tumour cells. 19715559592
[production of aspergillus oryzae mutants, active producers of acid proteinase]. 19715580123
conditions and course of accumulation and excretion of amylases in cultures of aspergillus oryzae. 19715112095
purification and properties of a new alpha-glucanase from aspergillus oryzae. 19715089323
[biosynthesis of glycoproteins. xvii. characterization of a mannosyltransferase from aspergillus oryzae]. 19714996438
purification and properties of an arylsulfatase from aspergillus oryzae. 19714998894
studies on the conidia of aspergillus oryzae. xi. latent ribonuclease in the conidia of aspergillus oryzae. 19715001286
properties of proteases from aspergillus oryzae and aspergillus flavus. a comparative study. 19714108471
[study of the structure of the enzyme complex from a culture of aspergillus oryzae 8f-1 by electrophoresis in polyacrylamide gel and immunoelectrophoresis]. 19715129018
synthesis of l-3,4-dihydroxyphenylalanine from l-tyrosine by microorganisms.l-3, 4-dihydroxyphenylalanine can be synthesized efficiently from l-tyrosine under acidic conditions by aspergillus oryzae and other fungi.19715130435
studies on the respiratory system of aspergillus oryzae. 3. properties of mitochondria from mycelia grown in the presence of antimycin a. 19714322618
influence of an enzyme from aspergillus oryzae, protease i, on some components of the fibrinolytic system. 19704246604
induction of enzymes in dormant spores of aspergillus oryzae.in dormant conidia of aspergillus oryzae, alpha-amylase, invertase, and glucose dehydrogenase were induced by their respective inducers. neither germination nor swelling occurred during this period.19705438036
[experimental pulmonary embolism in dogs. local administration of aspergillus-oryzae protease i]. 19705490745
[determination of optimal conditions for cultivation of aspergillus oryzae using mathematical methods of planning the experiments]. 19705497277
proteolytic inhibitors in plasma from man treated with a protease from aspergillus oryzae. 19705419701
studies on n-acetyl-beta-d-glucosaminidase of aspergillus oryzae. i. purification and characterization of n-acetyl-beta-d-glucosaminidase obtained from takadiastase. 19705452757
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