Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| lentinula edodes tlg1 encodes a thaumatin-like protein that is involved in lentinan degradation and fruiting body senescence. | lentinan is an antitumor product that is purified from fresh lentinula edodes fruiting bodies. it is a cell wall component, comprising beta-1,3-glucan with beta-1,6-linked branches, which becomes degraded during postharvest preservation as a result of increased glucanase activity. in this study, we used n-terminal amino acid sequence to isolate tlg1, a gene encoding a thaumatin-like (tl) protein in l. edodes. the cdna clone was approximately 1.0 kb whereas the genomic sequence was 2.1 kb, and co ... | 2006 | 16648221 |
| aspergillus oryzae strains with a large deletion of the aflatoxin biosynthetic homologous gene cluster differentiated by chromosomal breakage. | recently we divided aspergillus oryzae rib strains into group 1, having seven aflatoxin biosynthesis homologous genes (aflt, nor-1, aflr, nora, avna, verb, and vbs), and group 2, having three homologues (avna, verb, and vbs). here, partial aflatoxin homologous gene cluster of rib62 from group 2 was sequenced and compared with that of rib40 from group 1. rib62 showed a large deletion upstream of ver-1 with more than half of the aflatoxin homologous gene cluster missing including aflr, a positive ... | 2006 | 16673111 |
| molecular analysis of an inactive aflatoxin biosynthesis gene cluster in aspergillus oryzae rib strains. | to help assess the potential for aflatoxin production by aspergillus oryzae, the structure of an aflatoxin biosynthesis gene homolog cluster in a. oryzae rib 40 was analyzed. although most genes in the corresponding cluster exhibited from 97 to 99% similarity to those of aspergillus flavus, three genes shared 93% similarity or less. a 257-bp deletion in the aflt region, a frameshift mutation in nora, and a base pair substitution in vera were found in a. oryzae rib 40. in the aflr promoter, two s ... | 2006 | 16391082 |
| chemoprevention of mouse urinary bladder carcinogenesis by fermented brown rice and rice bran. | fermented brown rice by aspergillus oryzae (fbra) has been shown to be a potent anti-carcinogenic compound. here, we investigated the modifying effects of dietary feeding with a naturally occurring anti-oxidant fbra on n-butyl-n-(4-hydroxybutyl)-nitrosamine (oh-bbn)-induced urinary bladder carcinogenesis in male icr mice. five-week-old male icr mice were divided into 7 groups, and groups 1-5 were given oh-bbn (500 ppm) in drinking water for 6 weeks starting at 7 weeks of age. groups 2 and 3 were ... | 2006 | 16465408 |
| enhanced gene targeting frequency in ku70 and ku80 disruption mutants of aspergillus sojae and aspergillus oryzae. | in the koji molds aspergillus sojae and aspergillus oryzae, exogenous dna is integrated in the genome, in most cases irrespective of the sequence homology, suggesting that dna integration occurs predominantly through a nonhomologous end joining pathway where two ku genes, namely, ku70 and ku80, play a key role. to determine the effect of ku gene disruption on the gene targeting frequency, we constructed ku70-, ku80-, and ku70-ku80-disrupted strains of a. sojae and a. oryzae. the gene targeting f ... | 2006 | 16470383 |
| cloning and expression of 1,2-alpha-mannosidase gene (fmanib) from filamentous fungus aspergillus oryzae: in vivo visualization of the fmanibp-gfp fusion protein. | 1,2-alpha-mannosidase catalyzes the specific cleavage of 1,2-alpha-mannose residues from protein-linked n-glycan. in this study, a novel dna sequence homologous to the authentic 1,2-alpha-mannosidase was cloned from a cdna library prepared from solid-state cultured aspergillus oryzae. the fmanib cdna consisted of 1530 nucleotides and encoded a protein of 510 amino acids in which all consensus motifs of the class i alpha-mannosidase were conserved. expression of the full length of 1,2-alpha-manno ... | 2006 | 16495665 |
| proteomic analysis of extracellular proteins from aspergillus oryzae grown under submerged and solid-state culture conditions. | filamentous fungi are widely used for the production of homologous and heterologous proteins. recently, there has been increasing interest in aspergillus oryzae because of its ability to produce heterologous proteins in solid-state culture. to provide an overview of protein secretion by a. oryzae in solid-state culture, we carried out a comparative proteome analysis of extracellular proteins in solid-state and submerged (liquid) cultures. extracellular proteins prepared from both cultures sequen ... | 2006 | 16672490 |
| cladal relatedness among aspergillus oryzae isolates and aspergillus flavus s and l morphotype isolates. | aspergillus flavus is the main etiological agent for aflatoxin contamination of crops. its close relative, a. oryzae, does not produce aflatoxins and has been widely used to produce fermented foods. we compared the phylogeny of a. oryzae isolates and l- and s-type sclerotial isolates of a. flavus using single nucleotide polymorphisms in the omta gene in the aflatoxin biosynthesis gene cluster and deletions in and distal to the norb-cypa intergenic region as phylogenetic signals. aflatoxin-produc ... | 2006 | 16430983 |
| olive-mill wastewaters: a promising substrate for microbial lipase production. | the present study investigated the valorization of olive-mill wastewater (omw) by its use as a possible growth medium for the microbial production of extra-cellular lipase. to this end, strains of geotrichum candidum (nrrl y-552 and y-553), rhizopus arrhizus (nrrl 2286 and isrim 383), rhizopus oryzae (nrrl 6431), aspergillus oryzae (nrrl 1988 and 495), aspergillus niger (nrrl 334), candida cylindracea (nrrl y-17506) and penicillium citrinum (nrrl 1841 and 3754, isrim 118) were screened. all stra ... | 2006 | 16236495 |
| comparison of the aflr gene sequences of strains in aspergillus section flavi. | aflatoxins are polyketide-derived secondary metabolites produced by aspergillus parasiticus, aspergillus flavus, aspergillus nomius and a few other species. the toxic effects of aflatoxins have adverse consequences for human health and agricultural economics. the aflr gene, a regulatory gene for aflatoxin biosynthesis, encodes a protein containing a zinc-finger dna-binding motif. although aspergillus oryzae and aspergillus sojae, which are used in fermented foods and in ingredient manufacture, h ... | 2006 | 16385126 |
| g-protein and camp-mediated signaling in aspergilli: a genomic perspective. | we have carried out an in silico exploration of the genomes of aspergillus nidulans, aspergillus fumigatus, and aspergillus oryzae, and identified components of g-protein/camp-mediated signaling. putative g-protein coupled receptors (gpcrs) were distributed over nine classes. the gpcrs within classes were well conserved among aspergilli but varied in other ascomycetes. as previously observed in a. nidulans and other fungi, three galpha, one gbeta, and one ggamma subunits of g proteins were ident ... | 2006 | 16546420 |
| characterization of a periplasmic s1-like nuclease coded by the mesorhizobium loti symbiosis island. | dna sequences encoding hypothetical proteins homologous to s1 nuclease from aspergillus oryzae are found in many organisms including fungi, plants, pathogenic bacteria, and eukaryotic parasites. one of these is the m1 nuclease of mesorhizobium loti which we demonstrate herein to be an enzymatically active, soluble, and stable s1 homolog that lacks the extensive mannosyl-glycosylation found in eukaryotic s1 nuclease homologs. we have expressed the cloned m1 protein in m. loti and purified recombi ... | 2006 | 16529713 |
| differential distribution of the endoplasmic reticulum network as visualized by the bipa-egfp fusion protein in hyphal compartments across the septum of the filamentous fungus, aspergillus oryzae. | we visualized the endoplasmic reticulum (er) network by expression of the bipa-egfp fusion protein in the filamentous fungus, aspergillus oryzae, and focused on the spatial difference of the er distribution throughout hyphae. the er formed an interconnected network with motility and displayed a gradient distribution from the apical region. the er was also found as a tubular network along the septum, which was formed soon after the completion of septation. discontinuity of the er network distribu ... | 2006 | 16759887 |
| food-processing enzymes from recombinant microorganisms--a review. | enzymes are commonly used in food processing and in the production of food ingredients. enzymes traditionally isolated from culturable microorganisms, plants, and mammalian tissues are often not well-adapted to the conditions used in modern food production methods. the use of recombinant dna technology has made it possible to manufacture novel enzymes suitable for specific food-processing conditions. such enzymes may be discovered by screening microorganisms sampled from diverse environments or ... | 2006 | 16769167 |
| an enzymatically produced novel cyclomaltopentaose cyclized from amylose by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}. | a bacterial strain am7, isolated from soil and identified as bacillus circulans, produced two kinds of novel cyclic oligosaccharides. the cyclic oligosaccharides were produced from amylose using a culture supernatant of the strain as the enzyme preparation. the major product was a cyclomaltopentaose cyclized by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}. the other minor product was cyclomaltohexao ... | 2006 | 16545346 |
| deletion analysis of the superoxide dismutase (sodm) promoter from aspergillus oryzae. | the manganese superoxide dismutase gene (sodm) is very highly expressed in aspergillus oryzae. to elucidate the basis for this high-level expression, deletion analysis of the promoter was undertaken using beta-glucuronidase (gus) as a reporter. deletion of a 63-bp sequence from -200 to -138 in the 1,038-bp sodm promoter caused a drastic decrease in gus activity. in addition, an electrophoretic gel mobility shift assay (emsa) implicated a 30-bp element from -209 to -178 containing cis-element(s) ... | 2006 | 16547700 |
| substrate specificity of aspergillus oryzae family 3 beta-glucosidase. | among glycoside hydrolases, beta-glucosidase plays a unique role in many physiological and biocatalytical processes that involve the beta-linked o-glycosyl bond of various oligomeric saccharides or glycosides. structurally, the enzyme can be grouped into glycoside hydrolase family 1 and 3. although the basic ("retaining, double-displacement") mechanism for the catalysis of family 3 beta-glucosidase has been established, in-depth understanding of its structure-function relationship, particularly ... | 2006 | 16650812 |
| first report of a xylose-specific lectin with potent hemagglutinating, antiproliferative and anti-mitogenic activities from a wild ascomycete mushroom. | from fresh fruiting bodies of the wild ascomycete mushroom (xylaria hypoxylon) a lectin with n-terminal sequence resemblance to a part of aspergillus oryzae genome and only slight similarity to fungal immunomodulatory protein from the mushroom flammulina velutipes was isolated. the protocol comprised extraction with water, precipitation from the aqueous extract using 80% saturated (nh(4))(2)so(4), ion exchange chromatography on deae-cellulose and cm-cellulose, and then gel filtration by fast pro ... | 2006 | 16952421 |
| suppression on the mutagenicity of 4-nitroquinoline-n-oxide by the methanol extracts of soybean koji prepared with various filamentous fungi. | in this study, solid fermentation of soybean with various gras filamentous fungi including aspergillus sojae bcrc 30103, aspergillus oryzae bcrc 30222, aspergillus awamori, actinomucor taiwanesis and rhizopus sp. was performed to prepare various soybean kojis. toxicity, mutagenicity and suppression on the mutagenesis induced by a direct mutagen, 4-nitroquinoline-n-oxide (4-nqo) on salmonella typhimurium ta 100, by the various methanol extracts of the prepared soybean koji and unfermented soybean ... | 2006 | 16580082 |
| chemical components, palatability, antioxidant activity and antimutagenicity of oncom miso using a mixture of fermented soybeans and okara with neurospora intermedia. | the enzyme activities of aspergillus oryzae on koji (malted rice) and neurospora intermedia on s-oncom and o-oncom (fermented soybeans and okara with n. intermedia, respectively) were compared. the major enzymes of n. intermedia were different from those of a. oryzae, and the enzyme activities of o-oncom were extremely higher than those of s-oncom. s5-miso, s10-miso and s9o1-miso replacing 50% or 100% of steamed soybeans with s-oncom or a 9 : 1 mixture of s-oncom and o-oncom, respectively, were ... | 2006 | 16967767 |
| distribution profiles of isoflavone isomers in black bean kojis prepared with various filamentous fungi. | this study was conducted to compare the transformation of both isoflavone derivatives (aglycones, beta-glucosides, and acetyl and malonyl gluclucosides) and beta-glucosidase activity in kojis fermented with various generally recognized as safe filamentous fungi including aspergillus awamori, aspergillus oryzae, aspergillus sojae, rhizopus azygosporus, and rhizopus sp. no. 2. solid fermentation was performed to prepare the kojis by inoculating the steamed black beans with starter organism and cul ... | 2006 | 16478253 |
| novel hydrophobic surface binding protein, hsba, produced by aspergillus oryzae. | hydrophobic surface binding protein a (hsba) is a secreted protein (14.5 kda) isolated from the culture broth of aspergillus oryzae rib40 grown in a medium containing polybutylene succinate-co-adipate (pbsa) as a sole carbon source. we purified hsba from the culture broth and determined its n-terminal amino acid sequence. we found a dna sequence encoding a protein whose n terminus matched that of purified hsba in the a. ozyzae genomic sequence. we cloned the hsba genomic dna and cdna from a. ory ... | 2006 | 16597938 |
| development of aflp-derived, functionally specific markers for environmental persistence studies of fungal strains. | the ability to rapidly identify and quantify a microbial strain in a complex environmental sample has widespread applications in ecology, epidemiology, and industry. in this study, we describe a rapid method to obtain functionally specific genetic markers that can be used in conjunction with standard or real-time polymerase chain reaction (pcr) to determine the abundance of target fungal strains in selected environmental samples. the method involves sequencing of randomly cloned aflp (amplified ... | 2006 | 16699570 |
| [production of antitumor substances from shochu distillation remnants]. | this study aimed to develop a new processing method for the effective use of rice shochu distillation remnants. we examined the inhibitory effects on the growth of human lung carcinoma cells in the medium of rice shochu distillation remnants with various fungi. interestingly, high inhibitory effects on the growth of human lung carcinoma cells in the medium of rice shochu distillation remnants with aspergillus oryzae were obtained, although no inhibitory effect was observed in the case of synthet ... | 2006 | 16679744 |
| improvement of the glab promoter expressed in solid-state fermentation (ssf) of aspergillus oryzae. | the glucoamylase-encoding gene (glab) promoter should be very useful for recombinant protein production in solid-state fermentation (ssf) of aspergillus oryzae. a 97-bp fragment containing the cis-element of the glab promoter was inserted into the glaa promoter, which was little expressed in ssf. the chimeric promoter showed about a 24-fold increase in promoter activity in ssf. eight copies of the 97-bp fragment were tandemly fused with the glab promoter. the improved promoter showed about a 4.6 ... | 2006 | 16717420 |
| a traditional japanese medicine mitigates tnbs-induced colitis in rats. | differences in dietary habits may be one of the reasons that the incidence of inflammatory bowel disease has remained lower in japan than in western countries. we investigated whether a traditional japanese medicine (strong wakamto), based on aspergillus oryzae koji, would exert any effect on experimental colitis in rats. | 2006 | 16990203 |
| innovative effect of illite on improved microbiological conversion of l-tyrosine to 3,4 dihydroxy phenyl l-alanine (l-dopa) by aspergillus oryzae me2 under acidic reaction conditions. | in the present investigation, the previous ultraviolet irradiated mutant strain of aspergillus oryzae uv-7 was further improved in terms of 3,4 dihydroxy phenyl l-alanine (l-dopa) activity after chemical mutagenesis through 1-methyl 3-nitro 1-nitroso guanidine (mnng = 250-1500 microg/ml) treatment (0-30 min). among several mutant variants, the one that produced a larger amount of l-dopa from l-tyrosine was designated to as me2 and it was made 2-deoxy-d-glucose-resistant by growing it at various ... | 2006 | 17039388 |
| optimization of multienzyme production by two mixed strains in solid-state fermentation. | f(3) and f(4) strains of aspergillus niger were screened from five strains of fungi to produce multienzyme preparations (containing cellulase, hemicellulase, glucoamylase, pectinase, and acidic proteinase) as dietary supplementation. enzyme activities indicated that 1:4 (f(3) to f(4)) was the optimum mixture proportion, and 0.3% (w/w) was the preferable pitching rate. in bran mash containing 54.5% (w/w) water, f(3) and f(4) could produce the supplementation better when cultured 30 to 36 h at 30 ... | 2006 | 16802152 |
| expression of aspergillus hemoglobin domain activities in aspergillus oryzae grown on solid substrates improves growth rate and enzyme production. | dna fragments coding for hemoglobin domains (hbd) were isolated from aspergillus oryzae and aspergillus niger. the hbd activities were expressed in a. oryzae by introduction of hbd gene fragments under the control of the promoter of the constitutively expressed gpda gene. in the transformants, oxygen uptake was significantly higher, and during growth on solid substrates the developed biomass was at least 1.3 times higher than that of the untransformed wild-type strain. growth rate of the hbd-act ... | 2006 | 16927259 |
| a novel gh43 alpha-l-arabinofuranosidase from humicola insolens: mode of action and synergy with gh51 alpha-l-arabinofuranosidases on wheat arabinoxylan. | a novel alpha-l-arabinofuranosidase (alpha-araf) belonging to glycoside hydrolase (gh) family 43 was cloned from humicola insolens and expressed in aspergillus oryzae. (1)h-nmr analysis revealed that the novel gh43 enzyme selectively hydrolysed (1-->3)-alpha-l-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides. the optimal activity of the cloned enzyme was at ph 6.7 and 53 degrees c. two other novel alpha-l-arabinof ... | 2006 | 16944135 |
| impact of aspergillus oryzae genomics on industrial production of metabolites. | aspergillus oryzae is used extensively for the production of the traditional japanese fermented foods sake (rice wine), shoyu (soy sauce), and miso (soybean paste). in recent years, recombinant dna technology has been used to enhance industrial enzyme production by a. oryzae. recently completed genomic studies using expressed sequence tag (est) analyses and whole-genome sequencing are quickly expanding the industrial potential of the fungus in biotechnology. genes that have been newly discovered ... | 2006 | 16944282 |
| reduction of flatus-inducing factors in soymilk by immobilized alpha-galactosidase. | alpha-galactosidase from aspergillus oryzae was immobilized on chitosan beads using glutaraldehyde as a cross-linking agent. the general properties of free and immobilized enzymes were determined. the optimum ph for the free and immobilized enzymes was 4.8 and 4.6 respectively. the optimum temperature for the free enzyme was 50 degrees c, whereas that of immobilized enzyme was increased to 56 degrees c. kinetic parameters were determined with synthetic substrate (p-nitrophenyl alpha-d-galactopyr ... | 2006 | 16780418 |
| production of high-content galacto-oligosaccharide by enzyme catalysis and fermentation with kluyveromyces marxianus. | of three beta-galactosidases from aspergillus oryzae, kluyveromyces lactis and bacillus sp., used for the production of low-content galacto- oligosaccharides (gos) from lactose, the latter produced the highest yield of trisaccharides and tetrasaccharides. gos production was enhanced by mixing beta-galactosidase glucose oxidase. the low-content gos syrups, produced either by beta-galactosidase alone or by the mixed enzyme system, were subjected to the fermentation by kluyveromyces marxianus, wher ... | 2006 | 16786243 |
| impact of fermented brown rice with aspergillus oryzae intake and concentrations of organochlorine pesticides in blood of humans from japan. | 2006 | 16786444 | |
| proteomic methods applied to the analysis of immobilized biocatalysts. | methods adapted from proteomics can directly characterize proteins present in immobilized biocatalysts. complete hydrolysis followed by hplc analysis of tyr and phe estimates total protein bound, and is preferable to conventional difference methods, as tested with subtilisin carlsberg on silica. this new method shows that various treatments give quantitative desorption of proteins immobilized by adsorption. intact desorbed proteins may be analyzed by electrospray mass spectrometry. the candida a ... | 2006 | 16807923 |
| aovps24, a homologue of vps24, is required for vacuolar formation which could maintain proper growth and development in the filamentous fungus aspergillus oryzae. | vps24 (vacuolar protein sorting) is a component of escrt iii (endosomal sorting complex required for transport), which is required for the formation of mvb (multivesicular body). we have isolated the vps24 homologue gene, aovps24, from the filamentous fungus aspergillus oryzae, and analyzed the localization of aovps24 using egfp. aovps24 was localized in the cytoplasm and late endosome-like structures. furthermore, we constructed an aovps24 disruptant, which showed impaired growth, conidiation, ... | 2006 | 16857172 |
| an efficient two step purification and molecular characterization of beta-galactosidases from aspergillus oryzae. | beta-galactosidases (beta-d-galactoside-galactohydrolases (ec 3.2.1.23), lactases) are important industrial enzymes used for the hydrolysis of lactose from milk and milk whey. these enzymes are produced by different organisms and purified by multi-step procedures. the multi-step purification schemes are cost and time ineffective which can also lead to poor yield, denaturation and loss of enzymatic activity. in our study, extracellular beta-galactosidase from mutant strain aspergillus oryzaeh26-1 ... | 2006 | 16865665 |
| in vivo expression of udp-n-acetylglucosamine: alpha-3-d-mannoside beta-1,2-n-acetylglucosaminyltransferase i (gnt-1) in aspergillus oryzae and effects on the sugar chain of alpha-amylase. | udp-n-acetylglucosamine: alpha-3-d-mannoside beta-1,2-n-acetylglucosaminyltransferase i (gnt-i) is an essential enzyme in the conversion of high mannose type oligosaccharide to the hybrid or complex type. the full length of the rat gnt-i gene was expressed in the filamentous fungus aspergillus oryzae. a microsomal preparation from a recombinant fungus (strain ng) showed gnt-i activity that transferred n-acetylglucosamine residue to acceptor heptaose, man(5)glcnac(2). the n-linked sugar chain of ... | 2006 | 17090929 |
| possible involvement of pleiomorphic vacuolar networks in nutrient recycling in filamentous fungi. | morphological analyses of vacuoles in filamentous fungi in the past decade have led to the remarkable finding that they are highly pleiomorphic organelles. among them, tubular vacuoles have been implicated in nutrient transport between hyphal tips and the host plant surface in mycorrhizal fungi. however, a series of works suggested the presence of tubular vacuoles in other fungi that are not mycorrhizal, including aspergillus oryzae, hinting at more general roles of the tubular vacuoles. recentl ... | 2006 | 16874107 |
| development of a versatile expression plasmid construction system for aspergillus oryzae and its application to visualization of mitochondria. | we report here a development of the multisite gateway(tm)-based versatile plasmid construction system applicable for the rapid and efficient preparation of aspergillus oryzae expression plasmids. this system allows the simultaneous connection of the three dna fragments inserted in entry clones along with a destination vector in a defined order and orientation. we prepared a variety of entry clones and destination vectors containing promoters, genes encoding carrier-proteins and fusion tags, and ... | 2006 | 16880596 |
| enhanced antioxidative activity of soybean koji prepared with various filamentous fungi. | in the present study, soybean koji fermented with various gras filamentous fungi, including aspergillus sojae bcrc 30103, aspergillus oryzae bcrc 30222, aspergillus awamori, actinomucor taiwanensis and rhizopus sp. these organisms are commonly used as starters in the fermentation of many traditional, oriental food products. the growth of starter organisms, total phenolic content, and antioxidative activities of the methanol extract of these kojis are compared with specific reference to alpha-dip ... | 2006 | 16943061 |
| evaluation of certain food additives. | this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives, with a view to recommending acceptable daily intakes (adis) and to prepare specifications for the identity and purity of food additives. the first part of the report contains a general discussion of the principles governing the toxicological evaluation of food additives (including flavouring agents), assessments of intake, and the establishment and revision of spe ... | 2006 | 17069402 |
| identification of genes from aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture. | we identified 22 genes from aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture (mslc), among which ser/thr protein kinase (aopk1) and phosphatase (aoppt) genes were cloned. we also revealed that aopk1 encodes a protein with an n-terminal sequence 150 amino acid residues longer than that predicted from the registered sequence in genbank. | 2006 | 17189178 |
| expression of aspergillus oryzae phytase gene in aspergillus oryzae rib40 niad(-). | aspergillus oryzae rib40 niad(-) was transformed using a plasmid constructed with the a. oryzae phytase gene and pnan8142 vector. the culture broth of the transformant, which was grown in a medium containing starch as a carbon source and polyvinylpyrrolidone showed phytase activity of a maximum of 2.0 units ml(-1) at 37 degrees c, ph 5.5. | 2006 | 17270723 |
| high-throughput genotyping of filamentous fungus aspergillus oryzae based on colony direct polymerase chain reaction. | high-throughput genotyping of aspergillus oryzae was achieved using an fta card for the extraction of a genomic dna template for polymerase chain reaction from a fungal colony growing on an agar plate. this method was then applied to detect other fungal species from agar slants and food materials. this method offers a convenient tool for the genotyping of filamentous fungi without using an organic solvent or specialized equipment. | 2006 | 17270725 |
| functional analysis of the atg8 homologue aoatg8 and role of autophagy in differentiation and germination in aspergillus oryzae. | autophagy is a well-known degradation system, induced by nutrient starvation, in which cytoplasmic components and organelles are digested via vacuoles/lysosomes. recently, it was reported that autophagy is involved in the turnover of cellular components, development, differentiation, immune responses, protection against pathogens, and cell death. in this study, we isolated the atg8 gene homologue aoatg8 from the filamentous fungus aspergillus oryzae and visualized autophagy by the expression of ... | 2006 | 16896216 |
| change in hyphal morphology of aspergillus oryzae during fed-batch cultivation. | industrial enzymes are often produced by filamentous fungi in fed-batch cultivations. during cultivation, the different morphological forms displayed by the fungi have an impact on the overall production. the morphology of a recombinant lipase producing aspergillus oryzae strain was investigated during fed-batch cultivations. during the exponential batch phase of the fed-batch cultivations, the average hyphal length increased as did the number of tips per hyphal element. most striking was the fi ... | 2006 | 16133324 |
| functional characterization of delta9 and omega9 desaturase genes in mortierella alpina 1s-4 and its derivative mutants. | cloning and characterization of the delta9 desaturase (delta9i) gene of a fungus, mortierella alpina 1s-4, was previously reported. in this study, two genes encoding delta9 desaturase homologs were isolated from this fungus. one is a delta9 desaturase (delta9ii) that exhibits 86% amino acid sequence similarity to delta9i. functional analysis involving expression of the encoding gene in aspergillus oryzae revealed that delta9ii exhibits delta9 desaturase activity, 18:0 being converted to 18:1delt ... | 2006 | 16133334 |
| identification of secreted proteins of aspergillus oryzae associated with growth on solid cereal substrates. | filamentous growth of aspergillus oryzae on solid cereal substrates involves secretion of substrate converting enzymes and a solid substrate specific polarised hyphal growth phenotype. to identify proteins produced under these specific conditions, the extracts of a. oryzae grown on wheat-based media were analysed using n-terminal sequence analysis. in a submerged wheat-based growth medium of a. oryzae, besides alpha-amylase, also an arabinosidase and xylanase were abundantly produced. in the ext ... | 2006 | 16226332 |
| an aspergillus oryzae acetyl xylan esterase: molecular cloning and characteristics of recombinant enzyme expressed in pichia pastoris. | we screened 20,000 clones of an expressed sequence tag (est) library from aspergillus oryzae (http://www.nrib.go.jp/ken/est/db/index.html) and obtained one cdna clone encoding a protein with similarity to fungal acetyl xylan esterase. we also cloned the corresponding gene, designated as aoaxe, from the genomic dna. the deduced amino acid sequence consisted of a putative signal peptide of 31-amino acids and a mature protein of 276-amino acids. we engineered aoaxe for heterologous expression in p. ... | 2006 | 16129506 |
| hydrolase-catalyzed michael addition of imidazoles to acrylic monomers in organic medium. | hydrolase-catalyzed michael addition of imidazole derivatives to acrylic monomers in organic medium was described. a serial of n-substituted imidazole derivatives were successfully synthesized in moderate yields by the catalysis of hydrolases in organic medium. nine commercially available hydrolases from different sources were screened and all of them were found to be able to catalyze this type of addition reaction. the reaction yields depended on the solvent properties and the solvents with hig ... | 2006 | 16099065 |
| 5' untranslated region of the hsp12 gene contributes to efficient translation in aspergillus oryzae. | we describe a 5' untranslated region (5'utr) that dramatically increases the expression level of an exogenous gene in aspergillus oryzae. using a series of 5'utr::gus (uida) fusion constructs, we analyzed the translation efficiency of chimeric mrnas with different 5'utrs at different temperatures. we found that the 5'utr of a heat-shock protein gene, hsp12, greatly enhanced the translation efficiency of the chimeric gus mrna at normal temperature (30 degrees c). moreover, at high temperature (37 ... | 2006 | 16059686 |
| identification and analysis of ku70 and ku80 homologs in the koji molds aspergillus sojae and aspergillus oryzae. | ku genes play a key role in the non-homologous end-joining pathway. we have identified ku70 and ku80 homologs in the koji molds aspergillus sojae and aspergillus oryzae, and have constructed the disruption mutants of ku70, ku80, and ku70-80 to characterize the phenotypic change in these mutants. neither ku70- nor ku80-disrupted strains show hypersensitivity to the dna damaging agents methylmethane sulfonate (mms) and phleomycin. moreover, undesirable phenotypes, such as poor growth or repressed ... | 2006 | 16428831 |
| [blood pressure lowering tripeptides derived from milk protein]. | fermentation of milk by the lactobacillus helveticus lbk-16h or hydrolysis of casein by protease of aspergillus oryzae generates the tripeptide casokinines val-pro-pro and ile-pro-pro. these peptides inhibit the kininase ii (also known as angiotensin converting enzyme, ace). daily intake of these peptides in sufficient amounts in milk, fruit vegetable drinks or pills slightly decreases the blood pressure in hypertensive patients. the currently available milk peptides decrease the blood pressure ... | 2007 | 17323290 |
| screening of beta-fructofuranosidase-producing microorganisms and effect of ph and temperature on enzymatic rate. | seventeen different strains of filamentous fungi were grown in batch cultures to compare their abilities for the production of beta-fructofuranosidase. three of them, aspergillus oryzae ipt-301, aspergillus niger atcc 20611 and strain ipt-615, showed high production with total fructosyltransferase activity higher than 12,500 units l(-1). in addition, the beta-fructofuranosidases of those strains have a high fructosyltransferase activity-to-hydrolytic activity ratio. the temperature and ph effect ... | 2007 | 17375295 |
| improvement of the antitumor activity of black currant polysaccharide by an enzymatic treatment. | a polysaccharide-rich substance isolated from black currant, named cassis polysaccharide (caps), was partially digested with beta-galactosidase from aspergillus oryzae and its immunostimulatory activity was investigated. the in vitro cytokine-inducing effect of caps on raw264 cells was gradually decreased along with lowering of the average mw of caps. in vivo, partially digested caps with a mean mw of approximately 20,000 showed the most potent antitumor activity against ehrlich carcinoma in mic ... | 2007 | 17485844 |
| carbohydrate binding specificity of a fucose-specific lectin from aspergillus oryzae: a novel probe for core fucose. | the alpha1,6-fucosyl residue (core fucose) of glycoproteins is widely distributed in mammalian tissues and is altered under pathological conditions. a probe that specifically detects core fucose is important for understanding the role of this oligosaccharide structure. aleuria aurantia lectin (aal) and lens culimaris agglutinin-a (lca) have been often used as carbohydrate probes for core fucose in glycoproteins. here we show, by using surface plasmon resonance (spr) analysis, that aspergillus or ... | 2007 | 17383961 |
| individual and combined effects of physicochemical parameters on ellagitannin acyl hydrolase and ellagic acid production from ellagitannin by aspergillus oryzae. | the individual and interactive effects of physicochemical parameters on ellagitannin acyl hydrolase activity and ellagic acid production by aspergillus oryzae using ellagitannins from acorn fringe of oak as substrate were studied. ellagitannins concentration, incubation time were identified as important physicochemical parameters influencing the enzyme synthesis and the production accumulation, and the substrate concentration with initial ph was determined to has an interactive effect on the enz ... | 2007 | 17450459 |
| biotransformation of piceid in polygonum cuspidatum to resveratrol by aspergillus oryzae. | biotransformation of piceid in polygonum cuspidatum to resveratrol by aspergillus oryzae was investigated in this study. resveratrol is widely used in medicine, food, and cosmetic because of its pharmacological properties. however, it has a much lower content in plants compared with its glucoside piceid, which has a much lower bioavailability. traditionally, the aglycone is acquired by acid or enzymatic hydrolysis of its glucoside, but the violent condition and the acid pollution in hydrolytic r ... | 2007 | 17333175 |
| genomics of aspergillus oryzae. | the genome sequence of aspergillus oryzae, a fungus used in the production of the traditional japanese fermentation foods sake (rice wine), shoyu (soy sauce), and miso (soybean paste), has revealed prominent features in its gene composition as compared to those of saccharomyces cerevisiae and neurospora crassa. the a. oryzae genome is extremely enriched with genes involved in biomass degradation, primary and secondary metabolism, transcriptional regulation, and cell signaling. even compared to t ... | 2007 | 17341818 |
| fungal-stressed germination of black soybeans leads to generation of oxooctadecadienoic acids in addition to glyceollins. | microbial-stressed germination of black soybeans leads to generation of a group of oxylipins, oxooctadecadienoic acids (kodes, including 13- z, e-kode, 13- e, e-kode, 9- e, z-kode, and 9- e, e-kode), and their respective glyceryl esters in addition to glyceollins, a known phytoalexins present in wild and fungi-infected soybeans. four fungi, aspergillus niger, aspergillus oryzae, rhizopus oligosporus, and white rice yeast ( aspergillus niger wry), were applied to compare their efficiency on induc ... | 2007 | 17892258 |
| fermentation as a bio-process to obtain functional soybean flours. | the effect of fermentation on the antioxidant compounds [vitamins c and e, total phenolic compounds (tpc), and reduced glutathione (gsh)], and antioxidant capacity [superoxide anion scavenging activity (sod-like activity), peroxyl radical-trapping capacity (prtc), inhibition of phosphatidylcholine (pc) peroxidation, and trolox equivalent antioxidant capacity (teac)] of soybean (glycine max cv. merit) was studied. fermentation was carried out in solid state in cracked seeds inoculated with asperg ... | 2007 | 17907774 |
| effects of fermented soybean meal on digestive enzyme activities and intestinal morphology in broilers. | the experiment was performed to compare the effects of fermented soybean meal (fermented with aspergillus oryzae 3.042, fsbm) and soybean meal on digestive enzyme activities and intestinal morphology in broilers. three hundred twenty 1-d-old ross x ross male broiler chicks were randomly allocated into 2 dietary treatments for a 6-wk feeding trial, including 0- to 21-d and 21- to 42-d periods. at the end of each stage, 8 broilers of each treatment were killed, and pancreas, small intestine digest ... | 2007 | 17495085 |
| characterisation of 3-methylorcinaldehyde synthase (mos) in acremonium strictum: first observation of a reductive release mechanism during polyketide biosynthesis. | isolation and sequencing of a pks gene isolated from xenovulene-producing cultures of acremonium strictum indicated the presence of nt-, ks-, at-, pt-, c-met- and r-domains; heterologous expression in aspergillus oryzae resulted in the production of 3-methylorcinaldehyde, demonstrating the role of the terminal reductase domain in product release. | 2007 | 17912413 |
| structure of the dimeric n-glycosylated form of fungal beta-n-acetylhexosaminidase revealed by computer modeling, vibrational spectroscopy, and biochemical studies. | fungal beta-n-acetylhexosaminidases catalyze the hydrolysis of chitobiose into its constituent monosaccharides. these enzymes are physiologically important during the life cycle of the fungus for the formation of septa, germ tubes and fruit-bodies. crystal structures are known for two monomeric bacterial enzymes and the dimeric human lysosomal beta-n-acetylhexosaminidase. the fungal beta-n-acetylhexosaminidases are robust enzymes commonly used in chemoenzymatic syntheses of oligosaccharides. the ... | 2007 | 17509134 |
| analysis of expressed sequence tags from the fungus aspergillus oryzae cultured under different conditions. | we performed random sequencing of cdnas from nine biologically or industrially important cultures of the industrially valuable fungus aspergillus oryzae to obtain expressed sequence tags (ests). consequently, 21 446 raw ests were accumulated and subsequently assembled to 7589 non-redundant consensus sequences (contigs). among all contigs, 5491 (72.4%) were derived from only a particular culture. these included 4735 (62.4%) singletons, i.e. lone ests overlapping with no others. these data showed ... | 2007 | 17540709 |
| synthesis of p-nitrophenyl sulfated disaccharides with beta-d-(6-sulfo)-glcnac units using beta-n-acetylhexosaminidase from aspergillus oryzae in a transglycosylation reaction. | when alpha-d-glcnac-oc(6)h(4)no(2) -p and beta-d-(6-sulfo)-glcnac-oc(6)h(4)no(2)-p (2) were used as substrates, beta-n-acetylhexosaminidase from aspergillus oryzae transferred the beta-d-(6-sulfo)-glcnac(unit from 2 to alpha-d-glcnac-oc(6)h(4)no(2) -p to afford beta-d-(6-sulfo)-glcnac-(1-->4)-alpha-d-glcnac-oc(6)h(4)no(2)-p (3) in a yield of 94% based on the amount of donor, 2, added. beta-d-(6-sulfo)-glcnac-(1-->4)-alpha-d-glc-oc(6)h(4)no(2)-p (4) was obtained with alpha-d-glc-oc(6)h(4)no(2) -p ... | 2007 | 17492477 |
| the promoter activity of isovaleryl-coa dehydrogenase-encoding gene (ivda) from aspergillus oryzae is strictly repressed by glutamic acid. | we cloned the isovaleryl-coa dehydrogenase (ivd)-encoding gene from aspergillus oryzae. the promoter of ivda was subjected to beta-glucuronidase (gus) reporter assays in which certain amino acids were used as a major carbon source. l-leucine most strongly induced gus-activity, while in the case of l-glutamate, significantly low activity was found, indicating that ivda transcription was strongly repressed by glutamic acid. | 2007 | 17587691 |
| calcium alginate entrapped preparations of aspergillus oryzae beta galactosidase: its stability and applications in the hydrolysis of lactose. | insoluble concanavalin a-beta galactosidase complex was obtained by using jack bean extract and this complex was crosslinked with glutaraldehyde, in order to maintain the integrity of complex in the presence of its substrate or products. concanavalin a-beta galactosidase complex retained 92% of the initial enzyme activity whereas crosslinked complex showed 88% activity. entrapment of concanavalin a-beta galactosidase complex into calcium alginate beads provided suitability to use this preparatio ... | 2007 | 17298841 |
| tpr domain of ser/thr phosphatase of aspergillus oryzae shows no auto-inhibitory effect on the dephosphorylation activity. | a ser/thr phosphatase gene cloned from aspergillus oryzae, aoppt, revealed that the tetratricopeptide repeat (tpr) and catalytic domains of the full-length aoppt are located at the n- and c-terminal regions, respectively, similar to those of human ser/thr phosphatase 5 (pp5) and yeast ppt1. four different regions of aoppt, namely, a full-length polypeptide, the catalytic domain, the catalytic domain plus c-terminal 15 amino-acid residues and the tpr domain were expressed in escherichia coli and ... | 2007 | 17475320 |
| temperature impacts the multiple attack action of amylases. | the action pattern of several amylases was studied at 35, 50, and 70 degrees c using potato amylose, a soluble (red starch) and insoluble (cross-linked amylose) chromophoric substrate. with potato amylose as substrate, bacillus stearothermophilus alpha-amylase (bsta) and porcine pancreatic alpha-amylase displayed a high degree of multiple attack (dma, i.e., the number of bonds broken during the lifetime of an enzyme-substrate complex minus one), the fungal alpha-amylase from aspergillus oryzae a ... | 2007 | 17309295 |
| the glucoamylase-encoding gene (glab) is expressed in solid-state culture with a low water content. | solid-state culture encourages high-level enzyme secretion by aspergillus oryzae. using the real-time quantitative reverse transcriptase-polymerase chain reaction, we confirmed that expression of the glucoamylase-encoding gene in a. oryzae cultured in solid-state culture depends on the water content of the culture. | 2007 | 17617703 |
| the beta-1,3-exoglucanase gene exga (exg1) of aspergillus oryzae is required to catabolize extracellular glucan, and is induced in growth on a solid surface. | the biological role of exga (exg1), a secretory beta-1,3-exoglucanase of aspergillus oryzae, and the expression pattern of the exga (exg1) gene were analyzed. the exga disruptant and the exga-overexpressing mutant were constructed, and phenotypes of both mutants were compared. higher mycelial growth rate and conidiation efficiency were observed for the exga-overexpressing mutant than for the exga disruptant when beta-1,3-glucan was supplied as sole carbon source. on the other hand, no difference ... | 2007 | 17420593 |
| chemopreventive effect of fermented brown rice and rice bran on 4-nitroquinoline 1-oxide-induced oral carcinogenesis in rats. | the preventive effects of the dietary administration of brown rice and rice bran fermented with aspergillus oryzae (fbra) on oral carcinogenesis induced by 4-nitroquinoline 1-oxide (4-nqo) were investigated in male f344 rats. at 7 weeks of age, the animals were given 20 ppm 4-nqo in their drinking water for 8 weeks to induce tongue neoplasms. groups of rats were fed diets containing 5 or 10% fbra during the initiation or postinitiation phases of the 4-nqo-induced oral carcinogenesis. the other g ... | 2007 | 17342331 |
| mutagenic and antimutagenic effects of methanol extracts of unfermented and fermented black soybeans. | in this study, solid fermentation of steamed black soybean with various gras (generally recognized as safe) filamentious-fungi including aspergillus awamori, aspergillus oryzae bcrc 30222, aspergillus sojae bcrc 30103, rhizopus azygosporus bcrc 31158 and rhizopus sp. no. 2 was performed. mutagenicity and antimutagenicity of the methanol extracts of unfermented and fermented steamed black soybeans against 4-nitroquinoline-n-oxide (4-nqo), a direct mutagen and benzo[a]pyrene (b[a]p), an indirect m ... | 2007 | 17628128 |
| development of an efficient production method for beta-mannosidase by the creation of an overexpression system in aspergillus aculeatus. | to develop an overexpression system in aspergillus aculeatus in order to establish an efficient overproduction method of beta-mannosidase (manb). | 2007 | 17651209 |
| [expression of endopolygalacturonase a of aspergillus oryzae in escherichia coli]. | pectinases are mainly used in the food industry to clarify fruit juices and wine, improve oil extraction, remove the peel from the citrus fruit, increase the firmness of some fruits and degum fibres. the filamentous fungus aspergillus oryzae, used for the production of traditional fermented foods, only could produce less pectinases under general conditions. so far only a few of pgs expressed in yeast or e. coli were reported but they did not show higher activity. the cdna of mature pga (without ... | 2007 | 17366896 |
| aspergillus oryzae peritonitis in capd: case report and review of the literature. | fungal peritonitis is a rare, but serious, complication of continuous ambulatory peritoneal dialysis (capd). we report a case of peritonitis caused by aspergillus oryzae in a man on capd therapy who was treated successfully with amphotericin b and caspofungin, followed by itraconazole and removal of the peritoneal catheter. a oryzae was identified by using sequence analysis of the ribosomal dna genes. of 10 reported cases since 2003, the mortality rate was 30%. removal of the capd catheter and s ... | 2007 | 17472853 |
| aspergillus flavus: human pathogen, allergen and mycotoxin producer. | aspergillus infections have grown in importance in the last years. however, most of the studies have focused on aspergillus fumigatus, the most prevalent species in the genus. in certain locales and hospitals, aspergillus flavus is more common in air than a. fumigatus, for unclear reasons. after a. fumigatus, a. flavus is the second leading cause of invasive aspergillosis and it is the most common cause of superficial infection. experimental invasive infections in mice show a. flavus to be 100-f ... | 2007 | 17526826 |
| gene silencing by rna interference in the koji mold aspergillus oryzae. | we found the orthologous genes required for rna interference (rnai) in the aspergillus oryzae genome database, and constructed a set of tools for gene silencing using rnai in a. oryzae. this system utilizes compatible restriction enzyme sites so that only a single target gene fragment is required to create the hairpin rna cassette. for ease of handling, we also separated the construction of the hairpin rna cassette for the target gene from its subsequent introduction into the expression vector. ... | 2007 | 17213655 |
| development of recombinant aspergillus oryzae whole-cell biocatalyst expressing lipase-encoding gene from candida antarctica. | to expand the industrial applications of candida antarctica lipase b (calb), we developed aspergillus oryzae whole-cell biocatalyst expressing the lipase-encoding gene from c. antarctica. a. oryzae niad300, which was derived from the wild type strain rib40, was used as the host strain. the calb gene was isolated from c. antarctica cbs6678 and expression plasmids were constructed with and without secretion signal peptide. the lipase gene was expressed under the control of improved glaa and pno-81 ... | 2007 | 17225093 |
| profiling of glycosidase activities using coumarin-conjugated glycoside cocktails. | glycosidases are a large subgroup of carbohydrate-processing enzymes that hydrolytically cleave the glycosidic bond. glycans formed by the action of glycosidases are involved in various biological processes. genetic abnormalities in glycosidases are associated with inherited diseases. thus, characterization of the catalytic activities of glycosidases is of great importance. herein, we describe a simple and rapid approach for determining glycosidase activity profiles using coumarin-conjugated gly ... | 2007 | 17256948 |
| function analysis of stea homolog in aspergillus oryzae. | the asexual ascomycete aspergillus oryzae has an stea homolog in its genome. the stea homolog of a. oryzae was transcribed in the wild-type strain but stea disruption did not affect the phenotype of the disruptant strain. on the other hand, the stea-overexpressing strain showed the restriction of vegetative hyphal growth and conidiation, and the formation of balloon-shaped structures. the restriction of vegetative hyphal growth and conidiation were partially rescued by high osmolarity. in additi ... | 2007 | 17178246 |
| autophagy during conidiation and conidial germination in filamentous fungi. | filamentous fungi form aerial hyphae on solid medium, and some of these differentiate into conidiophores for asexual sporulation (conidiation). in the filamentous deuteromycete, aspergillus oryzae, aerial hyphae are formed from the foot cells and some differentiate into conidiophores, which are composed of vesicles, phialides and conidia. recently, we isolated the yeast atg8 gene homologue aoatg8 from a. oryzae, and visualized autophagy by the expression of an egfp (enhanced green fluorescent pr ... | 2007 | 17183223 |
| chemometric analysis of in-line multi-wavelength fluorescence measurements obtained during cultivations with a lipase producing aspergillus oryzae strain. | the filamentous fungus, aspergillus oryzae, was cultivated in batch and fed-batch cultivations in order to investigate the use of multi-wavelength fluorescence for monitoring course of events during filamentous fungi cultivations. the a. oryzae strain applied expressed a fungal lipase from thermomyces lanuginosus. spectra of multi-wavelength fluorescence were collected every 5 min with the bioview system (delta, denmark) and both explorative and predictive models, correlating the fluorescence da ... | 2007 | 16948165 |
| structure and characteristics of an endo-beta-1,4-glucanase, isolated from trametes hirsuta, with high degradation to crystalline cellulose. | trametes hirsuta produced cellulose-degrading enzymes when it was grown in a cellulosic medium such as avicel or wheat bran. an endo-beta-1,4-glucanase (theg) was purified from the culture filtrate, and the gene and the cdna were isolated. the gene consisted of an open reading frame encoding 384 amino acids, interrupted by 11 introns. the whole sequence showed high homology with that of family 5 glycoside hydrolase. the properties of the recombinant enzyme (reg) in aspergillus oryzae were compar ... | 2007 | 17928699 |
| [rapd analysis of aspergilli and its application in brewing industry]. | phylogenetic analysis of sixteen aspergilli was done by rapd technology, using aspergillus oryzae as3.951, aspergillus flavus gim3.18 and aspergillus sojae as3.495 as controls. first, genome dna of the sixteen test strains were prepared by improved extraction method, and their quality was verified by electrophoresis and spectrophotometry. they displayed an identical band (approximately 20 kb) in agarose gel electrophoresis, which conformed to the fact that these strains all belong to aspergillus ... | 2007 | 17672321 |
| purification, characterization, and substrate specificity of a glucoamylase with steroidal saponin-rhamnosidase activity from curvularia lunata. | it has been previously reported that a glucoamylase from curvularia lunata is able to hydrolyze the terminal 1,2-linked rhamnosyl residues of sugar chains at c-3 position of steroidal saponins. in this work, the enzyme was isolated and identified after isolation and purification by column chromatography including gel filtration and ion-exchange chromatography. analysis of protein fragments by maldi-tof/tof proteomics analyzer indicated the enzyme to be 1,4-alpha-d-glucan glucohydrolase ec 3.2.1. ... | 2007 | 17823796 |
| modifying effects of fermented brown rice on fecal microbiota in rats. | brown rice fermented by aspergillus oryzae (fbra) is a fiber-rich food. effects of dietary administration of fbra on rat fecal microbiota composition were examined. male wistar rats were fed a basal diet or a 5% fbra- or 10% fbra-containing diet, and fecal microbiota was analyzed by culture and terminal-restriction fragment length polymorphism (t-rflp) analysis. the viable cell number of lactobacilli significantly increased after feeding 10% fbra diet for 3 weeks compared with that in the basal ... | 2007 | 17826198 |
| development of high-throughput screening system by single-cell reaction using microchamber array chip. | to demonstrate the practical use of a novel high-throughput screening system by a single cell reaction for the rapid screening of combinatorially mutated beta-glucosidase 1 from aspergillus oryzae, a yeast cell chip microchamber array in combination with yeast cell surface engineering was successfully developed. | 2007 | 17964493 |
| reversible and irreversible unfolding of multi-domain proteins. | in contrast to single-domain proteins unfolding of larger multi-domain proteins is often irreversible. in a comparative case study on three different multi-domain proteins (phosphoglycerate kinase: pgk and two homologous alpha-amylases: taka and bla) we investigated properties of unfolded states and their ability to fold back into the native state. for this purpose guanidine hydrochloride, alkaline ph, and thermal unfolded states were characterized. structural alterations upon unfolding and refo ... | 2007 | 17964867 |
| identification and characterization of an intracellular lectin, calnexin, from aspergillus oryzae using n-glycan-conjugated beads. | recently, asparagine-linked oligosaccharides (n-glycans) have been found to play a pivotal role in glycoprotein quality control in the endoplasmic reticulum (er). in order to screen proteins interacting with n-glycans, we developed affinity chromatography by conjugating synthetic n-glycans on sepharose beads. using the affinity beads with the dodecasaccharide glc(1)man(9)glcnac(2), one structure of the n-glycans, a 75-kda protein, was isolated from the membranous fraction including the er in asp ... | 2007 | 17986781 |
| change in turnover capacity of crude recombinant dye-decolorizing peroxidase (rdyp) in batch and fed-batch decolorization of remazol brilliant blue r. | decolorization of the representative anthraquinone dye, remazol brilliant blue r (rbbr) was assessed to determine the practical potential of crude recombinant dye-decolorizing peroxidase generated by aspergillus oryzae (rdyp) in term of turnover capacity of rdyp. the turnover capacity, defined as the milligram of rbbr decolorized per unit of rdyp inactivated over the catalytic life time of rdyp, was quantified under condition by h(2)o(2) -mediated rdyp inactivation. in batch culture, equimolar b ... | 2007 | 17569040 |
| systematic analysis of snare localization in the filamentous fungus aspergillus oryzae. | in spite of their great importance for both applied and basic biology, studies on vesicular trafficking in filamentous fungi have been so far very limited. here, we identified 21 genes, which might be a total set, encoding putative snare proteins that are key factors for vesicular trafficking, taking advantage of available whole genome sequence in the filamentous fungus aspergillus oryzae. the subsequent systematic analysis to determine the localization of putative snares using egfp-fused chimer ... | 2007 | 17590362 |
| absence of mutagenicity, genotoxicity, and subchronic oral toxicity of touchi extract. | touchi, a traditional chinese food used mainly for seasoning is obtained by first steaming soybeans followed by fermentation with aspergillus oryzae (koji). a series of toxicological studies was conducted to evaluate the mutagenic and genotoxic potential and subchronic toxicity of a water extract of touchi, a known inhibitor of alpha -glucosidase activity. touchi extract (te) did not induce reverse mutations in salmonella typhimurium strains ta98, ta1537, ta100, ta1535, and escherichia coli wp2u ... | 2007 | 17963133 |
| microbial production of sensory-active miraculin. | miraculin (mcl), a tropical fruit protein, is unique in that it has taste-modifying activity to convert sourness to sweetness, though flat in taste at neutral ph. to obtain a sufficient amount of mcl to examine the mechanism involved in this sensory event at the molecular level, we transformed aspergillus oryzae by introducing the mcl gene. transformants were expressed and secreted a sensory-active form of mcl yielding 2 mg/l. recombinant mcl resembled native mcl in the secondary structure and t ... | 2007 | 17592723 |
| enhancement of substrate recognition ability by combinatorial mutation of beta-glucosidase displayed on the yeast cell surface. | recently, in family 3 beta-glucosidase (bgl), the catalytically important asp nucleophile has been identified in the sdw segment of the sdwg sequence by site-directed mutagenesis. however, the details about the roles of each amino acid residue of the sdwg sequence have not been investigated. w293 of the sdw segment, which is the residue next to the nucleophile (d292) in family 3 bgl, is very important for hydrolytic reaction as a binder to a substrate. g294 of the sdwg sequence might play an imp ... | 2007 | 17602218 |
| double disruption of the proteinase genes, tppa and pepe, increases the production level of human lysozyme by aspergillus oryzae. | in this study, we investigated the effects of proteinase gene disruption on heterologous protein production by aspergillus oryzae. the human lysozyme (hly) was selected for recombinant production as a model for the heterologous protein. a tandem hly construct fused with alpha-amylase (amyb) was expressed by a. oryzae in which the kex2 cleavage site was inserted at the upstream of hly. hly was successfully processed from amyb and produced in the medium. we performed a systematic disruption analys ... | 2007 | 17622525 |
| molecular cloning, and characterization of a modular acetyl xylan esterase from the edible straw mushroom volvariella volvacea. | a new volvariella volvacea gene encoding an acetyl xylan esterase (designated as vvaxe1) was cloned and expressed in pichia pastoris. the cdna contained an orf of 1047 bp encoding 349 amino acids with a calculated mass of 39 990 da. vvaxe1 is a modular enzyme consisting of an n-terminal signal peptide, a catalytic domain, and a cellulose-binding domain. the amino acid sequence of the enzyme exhibited a high degree of similarity to cinnamoyl esterase b from penicillium funiculosum, and acetyl xyl ... | 2007 | 17623028 |
| a proteomics strategy to discover beta-glucosidases from aspergillus fumigatus with two-dimensional page in-gel activity assay and tandem mass spectrometry. | economically competitive production of ethanol from lignocellulosic biomass by enzymatic hydrolysis and fermentation is currently limited, in part, by the relatively high cost and low efficiency of the enzymes required to hydrolyze cellulose to fermentable sugars. discovery of novel cellulases with greater activity could be a critical step in overcoming this cost barrier. beta-glucosidase catalyzes the final step in conversion of glucose polymers to glucose. despite the importance, only a few be ... | 2007 | 18020405 |