PMID(sorted ascending)
construction of an efficient expression system for aspergillus isopullulanase in pichia pastoris, and a simple purification method.aspergillus niger atcc 9642 isopullulanase (ipu) was heterologously expressed by pichia pastoris gs115 under three different signal sequences of saccharomyces cerevisiae acid phosphatase, s. cerevisiae alpha-factor prepro peptide, and a. niger isopullulanase. one-step purification using lectin con a affinity chromatography yielded recombinant ipu (ipu-pp) with high purity. ipu-pp had a higher carbohydrate content than native ipu and ipu-ao expressed in a. oryzae m-2-3. ipu-pp hydrolyzed various ...200312834298
authentic and recombinant bilirubin oxidases are in different resting forms.myrothecium verrucaria bilirubin oxidase expressed in aspergillus oryzae is in a resting form different from that of the authentic bilirubin oxidase, but reaches the resting form of the authentic enzyme after one cycle of reduction and reoxidation with dioxygen as shown by the absorption and electron paramagnetic resonance spectra.200312834300
cloning, sequencing, and heterologous expression of a cellobiohydrolase cdna from the basidiomycete corticium rolfsii.from a corticium rolfsii cdna library, a clone homologous to other fungal cellobiohydrolase (cbh1) genes was isolated using the polymerase chain reaction. in the nucleotide sequence, one 1.6 kb long open reading frame coding for a polypeptide of 530 amino acid residues was detected which showed 64% identity with cbh1 of phanerochaete chrysosporium. with expression of the 1.8 kb cdna using the aspergillus oryzae expression system, we detected microcrystalline cellulose (avicel) hydrolyzing activi ...200312843660
type iii cu mutants of myrothecium verrucaria bilirubin oxidase.type iii cu ligand, his456 and his458, of myrothecium verrucaria (mt-1) bilirubin oxidases (bo) [ec] were doubly mutated as to lys, asp, and val. in spite of perturbation of the type iii cu centers, these mutants were pale blue or colourless when isolated. however, they became intense blue on reaction with reducing agents such as dithionite, ascorbate, hexacyanoferrate(ii), and octacyanotangstate(iv) under air, or with an oxidizing agent such as hexacyanoferrate(iii), indicating that the ...200312869533
active elimination of causative pcdfs/dds congeners of yusho by one year intake of fbra in japanese people.thirty-five years have been passing since the outbreak of kanemi rice oil poisoning, namely, yusho in the western japan. however, even now the patients with yusho have been still suffering from several objective and subjective symptoms. in order to improve or, if possible, to cure the such symptoms, the most important therapeutic treatment is considered to actively excrete the most toxic causative pcdfs/dds congeners, that is, 2,3,4,7,8-pentachlorodibenzofuran (pencdf) and 1,2,3,6,7,8-hexachloro ...200312872712
novel role of cytoplasmic dynein motor in maintenance of the nuclear number in conidia through organized conidiation in aspergillus oryzae.cytoplasmic dynein is a minus-end-directed, microtubule-dependent motor protein complex. dhca, cytoplasmic dynein heavy chain in aspergillus oryzae, contained four p-loops involved in atp binding which were conserved as in cytoplasmic dynein heavy chains of other organisms. the amino acid sequence of a. oryzae dhca was similar to cytoplasmic dynein heavy chains from other organisms except for the n-terminus of saccharomyces cerevisiae dyn1. disruption of dhca gene in the region encoding four p-l ...200312878196
upregulation of promoter activity of the aspergillus oryzae xylanase gene by site-directed aspergillus oryzae, inductive expression of the xynf1 gene is mediated by a transcriptional activator, aoxlnr. promoter activity of the xynf1 gene, monitored by beta-galactosidase activity, was successfully upregulated by mutating two non-canonical aoxlnr binding sequences to what is thought to be the strongest sequence. transformants carrying three canonical binding sequences in the promoter region produced enzyme 2.8 times more active (33,000 units mg(-1) protein) than that of transformants ...200312882555
solid-phase reducing agents as alternative for reducing disulfide bonds in proteins.disulfide reduction of kluyveromyces lactis and aspergillus oryzae beta-galactosidases and beta-lactoglobulin was assessed. reduction was performed using one of two thiol-containing agents: dithiothreitol (dtt) or thiopropyl-agarose with a high degree of substitution (1000 micromol of sh groups/g of dried gel). both reductants allowed an increase of three- (for k. lactis beta-galactosidase) and fourfold (for a. oryzae beta-galactosidase) in the initial content of sh groups in the lactases. nearl ...200312909729
synthesis of fimh receptor-active manno-oligosaccharides by reverse hydrolysis using alpha-mannosidases from penicillium citrinum, aspergillus phoenicis and almond.recombinant penicillium citrinum alpha-1,2-mannosidase, expressed in aspergillus oryzae, was employed to carry out regioselective synthesis of alpha- d-mannopyranosyl-(1-->2)- d-mannose. yields (w/w) of 16.68% disaccharide, 3.07% trisaccharide and 0.48% tetrasaccharide were obtained, with alpha1-->2 linkages present at 98.5% of the total linkages formed. non-specific alpha-mannosidase from almond was highly efficient in reverse hydrolysis and oligosaccharide yields of 45-50% were achieved. the p ...200412910329
thermal stability of alpha-amylase from aspergillus oryzae entrapped in polyacrylamide determine the suitability as a time-temperature indicator for dielectric pasteurization processes, the thermal stability (50-75 degrees c) of aspergillus oryzae alpha-amylase immobilized in polyacrylamide gel in phosphate buffer, mashed potatoes, and minced shrimp was examined. changing the cross-linking agent concentration from 3.3 to 5.3% and adding 2% salt did not markedly affect the thermal stability of the immobilized alpha-amylase. thermal inactivation was first order, and immobilizatio ...200312926898
isofagomine lactams, synthesis and enzyme inhibition.the synthesis of isofagomine lactams (2-oxoisofagomines) corresponding to the biologically important hexoses is presented. the d-glucose/d-mannose analogue (3s,4r,5r)-3,4-dihydroxy-5-hydroxymethylpiperidin-2-one (9) was synthesised in 9 steps from d-arabinose, the d-galactose analogue (3s,4s,5r)-3,4-dihydroxy-5-hydroxymethylpiperidin-2-one (10) was synthesised in 11 steps from d-arabinose and the l-fucose analogue (3r,4r,5r)-3,4-dihydroxy-5-methylpiperidin-2-one (11) was synthesised in 12 steps ...200312929423
preferential binding of two compatible solutes to the glycan moieties of peniophora lycii phytase.regulation of hydration behavior, and the concomitant effects on solubility and other properties, has been suggested as a main function of protein glycosylation. in this work, we have studied the hydration of the heavily glycosylated peniophora lycii phytase in solutions (0.15-1.1 m) of the two compatible solutes glycerol and sorbitol. osmometric measurements showed that glycerol preferentially binds to phytase (i.e., glycerol-glycoprotein interactions are more favorable than water-glycoprotein ...200312939159
the action of transglucosidase of aspergillus oryzae on maltose. 195212980966
transfructosidation reactions of an enzyme of aspergillus oryzae. 195212999833
[study of amino acid metabolism in aspergillus oryzae. i. effect of ph on free amino acids of aspergillus oryzae cultured on casamino acids and on glutamic acid]. 195313051119
transferase activity of the beta-glucosidases of aspergillus oryzae. 195313051324
factors influencing the paper chromatography of some enzymes of aspergillus oryzae and related enzymes. 195313051325
[study of amino acid metabolism in aspergillus oryzae. ii. free amino acids of mycelium cultured in different amino acids]. 195313080989
enzymes of aspergillus oryzae. iii. the sequence of appearance and some properties of the enzymes liberated during growth. 195313093530
enzymes of aspergillus oryzae. iv. fractionation and preparation of crystals rich in protease. 195313093531
enzymes of aspergillus oryzae. v. ethanol fractionation at low ionic strengths. 195313093532
[fungi from the family aspergillus as producers of amylase. 2. activity of amylase from aspergillus oryzae and its relation to growth on non-liquid natural media]. 195313119390
[production of amylase of molds from the family of aspergillus. 3. effect of conditions of growth on conidia formation in aspergillus oryzae]. 195313144397
[studies on amino acid metabolism by aspergillus oryzae. iii. free amino acids in the mycelium cultured on various sources of carbon and in the presence of ammonia]. 195413181389
[study on the metabolism of amino acids by aspergillus oryzae. iv. effect of calcium chloride on metabolism of amino acids]. 195413181392
[variability of aspergillus oryzae]. 195413223534
[presence of amylase in drops of exudates formed in colonies of aspergillus niger and aspergillus oryzae]. 195513312080
[effect of oxidized and reduced forms of nitrogen in culture medium on secretion of amylase from aspergillus oryzae]. 195613378073
[effect of phosphates on activity and content of amylolytic enzymes in aspergillus oryzae]. 195613407443
[adaptive characteristics of amylase of aspergillus oryzae]. 195713440595
[effect of osmotic pressure on formation of amylase by aspergillus oryzae]. 195713483125
effect of lytic enzyme from bacillus circulans and chitinase from streptomyces sp. on aspergillus oryzae. 195913622735
[adaptability of aspergillus oryzae amylolytic enzymes]. 195913643760
[a new method of hydrolysis of combined forms of vitamin b6 with an enzymatic preparation from aspergillus oryzae]. 196013687626
growth of aspergillus oryzae in irradiated sucrose-nitrate medium. 196113699378
effect of irradiation on growth and enzyme activity of aspergillus oryzae. 196113699379
[effect of an enzymatic preparation from aspergillus oryzae on some cardiac glycosides]. 196113710100
[hexokinase from aspergillus oryzae]. 196113744680
[studies on the production of fungal amylases. ii. morphology of 4 strains of aspergillus oryzae and the effect of the time of growth on their amylolytic activity]. 196013754977
[variability of experimentally produced forms of aspergillus oryzae]. 196113775438
[amylolytic activity of aspergillus oryzae growing on synthetic media]. 195913822165
[adaptation of aspergillus oryzae producing amylase]. 196013829342
the amino acid composition of alpha-amylase from aspergillus oryzae. 196013834098
[induced synthesis of amylase by the mycelium aspergillus oryzae]. 196013838380
[production of crystalline alpha-amylase by aspergillus oryzae]. 196113892280
[studies on granules and lipids in aspergillus oryzae cells]. 196113901919
influence of the size of inoculum on various growth phases in aspergillus oryzae. 196313935366
maltoryzine, a new toxic metabolite produced by a strain of aspergillus oryzae var. microsporus isolated from the poisonous malt sprout. 196213956269
purification and some properties of cytochromes from aspergillus oryzae. 196314060390
size of inoculum and nitrogen metabolism in aspergillus oryzae. 196314072836
[glucose inhibition of induced amylase synthesis in aspergillus oryzae]. 196314074531
preparation of mammalian cell cultures with enzyme from aspergillus oryzae. 196314076861
binding of alpha-amylase to the cell wall of aspergillus oryzae. 196314089480
localization of cell-bound alpha-amylase in aspergillus oryzae demonstrated by fluorescent-antibody technique. 196414102858
[on the nature of glucose inhibition of induced amylase synthesis in aspergillus oryzae]. 196414150175
studies on the thrombolytic activity of a protease from aspergillus oryzae. 196414157540
studies of the spore coats of fungi. i. isolation and composition of the spore coats of aspergillus oryzae. 196414200685
interdependence of inoculum size and trace element supply in growth of aspergillus oryzae. 196414245147
mannitol and mannitol dehydrogenases in conidia of aspergillus oryzae.horikoshi, koki (the institute of physical and chemical research, tokyo, japan), shigeji iida, and yonosuke ikeda. mannitol and mannitol dehydrogenases in conidia of aspergillus oryzae. j. bacteriol. 89:326-330. 1965.-a sugar alcohol was isolated from the conidia of aspergillus oryzae and identified as d-mannitol. two types of d-mannitol dehydrogenases, nicotinamide adenine dinucleotide phosphate-linked and nicotinamide adenine dinucleotide-linked, were found in the conidia. substrate specificit ...196514255698
the major alkaline proteinase of aspergillus oryzae, aspergillopeptidase b. i. isolation in homogeneous form. 196414269301
the major alkaline proteinase of aspergillus oryzae, aspergillopeptidase b. ii. partial specific volume, molecular weight, and amino acid composition. 196414269302
size of inoculum, stimulation, and inhibition of growth in aspergillus oryzae. 196514290961
the evaluation in the dog of a proteolytic enzyme derived from aspergillus oryzae. 196414324141
the action of a proteolytic enzyme from aspergillus oryzae on components of the blood clotting system. 196414324171
[biochemical and physiological characteristics of the fungus aspergillus oryzae and effect of conditions of its cultivation in the industry on the synt esis of amylolytic and proteolytic enzymes]. 195914404082
heterosis observed in aspergillus oryzae and its significance in koji manufacture. 195914409317
selection of fitter type nuclei in heterocaryons of aspergillus oryzae. 195914409318
x-ray diffraction patterns of the cell wall of aspergillus oryzae. 196214448908
[on the cytochemistry of polyphosphates in aspergillus oryzae]. 196214452453
study of the inhibition of four alpha amylases by acarbose and its 4iv-alpha-maltohexaosyl and 4iv-alpha-maltododecaosyl analogues.acarbose analogues, 4iv-maltohexaosyl acarbose (g6-aca) and 4iv-maltododecaosyl acarbose (g12-aca), were prepared by the reaction of cyclomaltodextrin glucanyltransferase with cyclomaltohexaose and acarbose. the inhibition kinetics of acarbose and the two acarbose analogues were studied for four different alpha-amylases: aspergillus oryzae, bacillus amyloliquefaciens, human salivary, and porcine pancreatic alpha-amylases. the three inhibitors showed mixed, noncompetitive inhibition, for all four ...200314499573
protein purification, cdna cloning and characterization of a protease inhibitor from the indian tasar silkworm, antheraea inhibitor of aspergillus oryzae fungal protease was purified to homogeneity from the hemolymph of fifth instar larvae of antheraea mylitta by ammonium sulfate precipitation, anion exchange and gel filtration (fplc) chromatography, and termed as amfpi-1. the extent of purification was checked by two-dimensional gel electrophoresis, and the molecular weight of purified inhibitor was determined by sds-page as 10.4 kda. fifteen n-terminal amino acid sequences of this protein were determined, and ...200314505696
secretory production of aspergillus oryzae xylanase xynf1, xynf1 cdna product, in the basidiomycete coprinus cinereus.the signal peptide of aspergillus oryzae endo-(1,4)-beta-xylanase xynf1 contains a c-terminal serine-arginine that directs efficient secretion of the enzyme into the culture medium. in the basidiomycete coprinus cinereus, however, there is little secretion of xynf1 into the culture medium. modification of the c-terminal sequence of the signal peptide to lysine-arginine resulted in efficient secretion of c. cinereus xynf1, suggesting the presence of a kex2-like protease in this fungus.200414513296
[analysis of kojic acid in aspergillus oryzae ferment by ion-pair reversed-phase high performance liquid chromatography].a new and an efficient method for analyzing kojic acid in aspergillus oryzae ferment by ion-pair reversed-phase high performance liquid chromatography was developed. the kojic acid was well separated on a c18 column using methanol-0.01 mol/l disodium hydrogen phosphate and 2 mmol tetrabutylammonium bromide (4:96) as the mobile phase, followed by an uv detection at 226 nm. the detection limit was 0.012 microgram/ml. the spiked recoveries in wort-yeast culture, wort-peptone culture and potato-yeas ...200314535109
growth estimation of solid-state koji by covering a cellophane membrane on the a solid-substrate fermentation system, fungal growth within a solid mash is an important index for the efficiency of the saccharification and production of metabolites. estimation of fungal mass in such a heterogeneous solid-substrate systems is difficult and tedious. in this work, the comparison of aspergillus oryzae which is a common strain for the wine-brewing process cultured on a cellophane membrane placed on a koji juice agar medium and a small scale of steamed rice koji culture was con ...199314545687
homogeneous batch cultures of aspergillus oryzae by elimination of wall growth in the variomixing bioreactor.a novel principle for mixing and aeration in stirred bioreactors, named variomixing, was developed. four baffles are rotated intermittently at a rotational speed slower or similar to the speed of a centrally placed axial flow impeller. rotational speeds of the baffles and impeller of 5-10 and 500-600 rpm, respectively, results in the highly turbulent flow regime characteristic of conventional bioreactors with high mixing and mass transfer capacities. stagnant zones around crevices and crannies i ...200414556039
natural and recombinant fungal laccases for paper pulp bleaching.three laccases, a natural form and two recombinant forms obtained from two different expression hosts, were characterized and compared for paper pulp bleaching. laccase from pycnoporus cinnabarinus, a well known lignolytic fungus, was selected as a reference for this study. the corresponding recombinant laccases were produced in aspergillus oryzae and a. niger hosts using the laci gene from p. cinnabarinus to develop a production process without using the expensive laccase inducers required by t ...200414600793
cloning, heterologous expression, and characterization of thielavia terrestris glucoamylase.thielavia terrestris is a soil-borne thermophilic fungus whose molecular/ cellular biology is poorly understood. only a few genes have been cloned from the thielavia genus. we detected an extracellular glucoamylase in culture filtrates of t. terrestris and cloned the corresponding glaa gene. the coding region contains five introns. based on the amino acid sequence, the glucoamylase was 65% identical to neurospora crassa glucoamylase. sequence comparisons suggested that the enzyme belongs to the ...200314665735
thiamine-regulated gene expression of aspergillus oryzae thia requires splicing of the intron containing a riboswitch-like domain in the 5'-utr.exogenous thiamine regulates aspergillus oryzae thia, which is involved in thiamine synthesis. one of the two introns in its 5'-untranslated region (5'-utr) contains motifs (regions a and b) highly conserved among fungal thiamine biosynthesis genes. deletion of either region relieved the repression by thiamine and thiamine inhibited intron splicing, suggesting that regions a and b are required for efficient splicing. furthermore, transcript splicing was essential for thia gene expression. these ...200314675766
a unique dye-decolorizing peroxidase, dyp, from thanatephorus cucumeris dec 1: heterologous expression, crystallization and preliminary x-ray analysis.the dye-decolorizing peroxidase dyp is a key enzyme in the decolorizing fungus thanatephorus cucumeris dec 1 that degrades azo and antraquinone dyes. the gene dyp from t. cucumeris dec 1, which has low homology to other peroxidase genes, was cloned and transformed into aspergillus oryzae and glycosylated dyp was expressed at high levels. purified dyp was deglycosylated using gst endo f1 and then crystallized in a strong magnetic field (10 t) at 283 k using ammonium sulfate as precipitant. x-ray ...200414684913
transformation of aspergillus aculeatus using the drug resistance gene of aspergillus oryzae and the pyrg gene of aspergillus nidulans.transformation systems for aspergillus aculeatus has been developed, based on the use of the pyrithiamine resistance gene of aspergillus oryzae and the orotidine-5'-monophosphate decarboxylase gene (pyrg) of aspergillus nidulans. an a. aculeatus mutant which can be transformed effectively by the a. nidulans pyrg gene was isolated as a transformation host. this is the first report of transformation of a. aculeatus.200314730150
maturation of fermented rice-koji miso can be monitored by an increase in fatty acid ethyl ester.a mixture of steamed soybean and boiled rice with seeded aspergillus oryzae was naturally fermented without addition of yeasts or lactobacilli, and kept matured for 12 months at room temperature. chemical analysis of this rice-koji miso sample for lipid changes during maturation showed that triacylglycerol was gradually decomposed into free fatty acid, with distinct formation of fatty acid ethyl ester which, six months after the start of fermentation, came to account for 35.0% of total lipid. th ...200414745195
new cationic exchanger support for reversible immobilization of tailor-made cationic exchange resins have been prepared by covalently binding aspartic-dextran polymers (e.g. mw 15 000-20 000) to porous supports (aminated agarose and sepabeads). more than 80% of the proteins contained in crude extracts from escherichia coli and acetobacter turbidans have been strongly adsorbed on these porous materials at ph 5. this interaction was stronger than in conventional carboxymethyl cellulose (e.g., at ph 7 and 25 degrees c, all proteins previously adsorbed at ph ...200414763854
multiplication of an ancestral gene encoding secreted fungalysin preceded species differentiation in the dermatophytes trichophyton and microsporum.dermatophytes are human and animal pathogenic fungi which cause cutaneous infections and grow exclusively in the stratum corneum, nails and hair. in a culture medium containing soy proteins as sole nitrogen source a substantial proteolytic activity was secreted by trichophyton rubrum, trichophyton mentagrophytes and microsporum canis. this proteolytic activity was 55-75 % inhibited by o-phenanthroline, attesting that metalloproteases were secreted by all three species. using a consensus probe co ...200414766908
crystallization of the -amylase of aspergillus oryzae. 195114882291
multiple nature of the enzymes of aspergillus oryzae. 195214919612
enzymes of aspergillus oryzae. i. the development of a culture medium yielding high protease activity. 195214934632
enzymes of aspergillus oryzae. ii. the yield of enzymes from mutants produced by ultraviolet irradiation. 195214934633
biotechnological advantages of laboratory-scale solid-state fermentation with fungi.despite the increasing number of publications dealing with solid-state (substrate) fermentation (ssf) it is very difficult to draw general conclusion from the data presented. this is due to the lack of proper standardisation that would allow objective comparison with other processes. research work has so far focused on the general applicability of ssf for the production of enzymes, metabolites and spores, in that many different solid substrates (agricultural waste) have been combined with many d ...200414963614
fermentation of seeds of teff (eragrostis teff), grass-pea (lathyrus sativus), and their mixtures: aspects of nutrition and food safety.fermentation of pure teff (eragrostis teff), pure grass-pea (lathyrus sativus), and their mixtures, 9:1 and 8:2 (teff/grass-pea) has been done at two temperatures (room temperature and 35 degrees c) in duplicate using the strains of lactobacillus plantarum, for bacterial fermentation, and aspergillus oryzae and rhizopus oligosporus in succession for solid-state fungal fermentation as inocula. in addition, the natural or spontaneous and back-slopping methods of bacterial fermentation have been do ...200414995115
novel nmr approach to assessing gene transfection: 4-fluoro-2-nitrophenyl-beta-d-galactopyranoside as a prototype reporter molecule for beta-galactosidase.gene therapy holds great promise for the treatment of diverse diseases. however, widespread implementation is hindered by difficulties in assessing the success of transfection in terms of spatial extent, gene expression, and longevity of expression. the development of noninvasive reporter techniques based on appropriate molecules and imaging modalities may help to assay gene expression. 4-fluoro-2-nitrophenyl-beta-d-galactopyranoside (pfonpg) is a novel prototype nmr-sensitive molecule, which is ...200415004806
rnase t1 variant rv cleaves single-stranded rna after purines due to specific recognition by the asn46 side chain amide.attempts to alter the guanine specificity of ribonuclease t1 (rnase t1) by rational or random mutagenesis have failed so far. the rnase t1 variant rv (lys41glu, tyr42phe, asn43arg, tyr45trp, and glu46asn) designed by combination of a random and a rational mutagenesis approach, however, exhibits a stronger preference toward adenosine residues than wild-type rnase t1. steady state kinetics of the cleavage reaction of the two dinucleoside phosphate substrates adenylyl-3',5'-cytidine and guanylyl-3' ...200415005620
amylose formulations for drug delivery to the colon: a comparison of two fermentation models to assess colonic targeting performance in vitro.the purpose of this study was to develop an enzyme-based fermentation system for the in vitro assessment of colonic digestion of amylose films and coatings, and to compare its performance with a conventional fermentation model inoculated with human faecal bacteria. amylose and ethylcellulose were mixed in different ratios and cast as isolated films, as well as spray coated onto drug-(5-aminosalicylic acid) loaded pellets. four commercial amylase enzymes were individually screened for their abili ...200415010137
production of toxic metabolites in aspergillus niger, aspergillus oryzae, and trichoderma reesei: justification of mycotoxin testing in food grade enzyme preparations derived from the three fungi.aspergillus niger, aspergillus oryzae, and trichoderma reesei are three important production organisms used in industrial fermentations. several of the fungal secondary metabolites produced by selected strains of these three fungi are capable of eliciting toxicity in animals. among those toxic substances are the well-known mycotoxins 3-nitropropionic acid and ochratoxin a. however, many others, such as kojic acid, may not be true mycotoxins. the production, extraction, chemical structure, and th ...200415041150
coconut oil cake--a potential raw material for the production of alpha-amylase.solid-state fermentation (ssf) was carried out using coconut oil cake (coc) as substrate for the production of alpha-amylase using a fungal culture of aspergillus oryzae. raw coc supported the growth of the culture, resulting in the production of 1372 u/gds alpha-amylase in 24 h. process optimization using a single parameter mode showed enhanced enzyme titre, which was maximum (1827 u/gds) when ssf was carried out at 30 degrees c for 72 h using a substrate with 68% initial moisture. supplementat ...200415051078
enantioselective transesterification using immobilized aspergillus oryzae overexpressing the present study, we used gene manipulation to construct a recombinant aspergillus oryzae strain overexpressing lipase and investigated its application to the optical resolution of chiral compounds. a. oryzae niad300, which was derived from the wild-type strain rib40, was used as the host strain. the tgla gene, which encodes a triacylglycerol lipase, was cloned from the a. oryzae niad300 chromosomal genome, then reintroduced, with and without a secretion-signal sequence, into the genome and ...200415052375
adenine auxotrophic mutants of aspergillus oryzae: development of a novel transformation system with triple auxotrophic hosts.adea and adeb genes homologous to saccharomyces cerevisiae ade1 and ade2, respectively, were cloned from aspergillus oryzae. adea and adeb share 62.8% and 52.5% identities with s. cerevisiae ade1 and ade2, respectively. in order to obtain triple auxotrophic mutants from a. oryzae, 12 red-colored mutant colonies were isolated by uv mutagenesis of a double auxotrophic host, ns4 (niad(-), sc(-)), as a parent strain. all the mutants exhibited adenine auxotrophy and showed fluorescence in the vacuole ...200415056900
injectable controlled release formulations incorporating protein crystals.development of ready-to-inject in situ formable controlled release gel systems for proteins is extremely challenging due to poor stability of proteins in the organic solvents typically used to fabricate these systems and because of the need of initial drying of proteins. the focus of the present study was to develop and characterize injectable controlled release systems composed of crystals of amylase, a model protein, suspended in solutions of polymeric and non-polymeric matrix materials in org ...200415063037
polyol accumulation by aspergillus oryzae at low water activity in solid-state fermentation.polyol accumulation and metabolism were examined in aspergillus oryzae cultured on whole wheat grains or on wheat dough as a model for solid-state culture. in solid-state fermentation (ssf), water activity (a(w)) is typically low resulting in osmotic stress. in addition to a high level of mannitol, which is always present in the cells, a. oryzae accumulated high concentrations of glycerol, erythritol and arabitol at relatively low a(w) (0.96-0.97) in ssf. accumulation of such a mixture of polyol ...200415073319
combined discrete particle and continuum model predicting solid-state fermentation in a drum fermentor.the development of mathematical models facilitates industrial (large-scale) application of solid-state fermentation (ssf). in this study, a two-phase model of a drum fermentor is developed that consists of a discrete particle model (solid phase) and a continuum model (gas phase). the continuum model describes the distribution of air in the bed injected via an aeration pipe. the discrete particle model describes the solid phase. in previous work, mixing during ssf was predicted with the discrete ...200415112293
thermal stabilization of penicillolysin, a thermolabile 19 kda zn2+-protease, obtained by site-directed mutagenesis.penicillolysin is a member of the clan mx and the family of m35 proteases. the enzyme is a thermolabile zn(2+)- protease from penicillium citrinum with a unique substrate profile. we expressed recombinant penicillolysin in aspergillus oryzae and generated several site-directed mutants, r33e/e60r, a167e and t81p, with the intention of exploring thermal stabilization of this protein. we based our choice of mutations on the structures of homologous thermally stable enzymes, deuterolysin (ec 3.4.24. ...200415115852
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