Publications
Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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optical resolution of dl-alanine by using immobilized aspergillus oryzae cells. | mycelium pellets with diameters of 1-2 mm and containing abundant aminoacylase were obtained by the liquid fermentation of aspergillus oryzae 3042. by the cross-linking method with reagents of gelatin and formaldehyde, the immobilized a. oryzae cells (iac) were prepared with much higher activity and reactive properties. the effects of factors on enzymatic activity of iac were investigated. when substrate concentration was less than 0.15 mol/l, the michaelis-menten mechanism was suitable for this ... | 1997 | 9343710 |
cloning and sequence analysis of the gene (epra1) encoding an extracellular protease from aeromonas hydrophila. | a gene (epra1) encoding the extracellular protease of aeromonas hydrophila ah1 has been cloned and sequenced. nucleotide sequence analysis of epra1 predicted a single open reading frame (orf) of 1038 bp encoding a 346 amino acid (aa) polypeptide, with a potential 21-aa signal peptide. when the epra1 gene was expressed in minicells, one major band of approx. 37 kda was identified, while protease activity staining experiments identified a caseinolytic band of approx. 29 kda determined by sds-page ... | 1997 | 9358060 |
effect of steam-flaked or steam-rolled corn with or without aspergillus oryzae in the diet on performance of dairy cows fed during hot weather. | the objective of this study was to determine the effects of steam-rolled versus steam-flaked corn in the diet with or without the addition of a culture of aspergillus oryzae on the performance of high producing dairy cows during hot summer weather. thirty-two holstein cows averaging 92 (+/- 60) d in milk were fed a pretreatment diet for 21 d followed by a 70-d experimental period in a completely randomized block design with a 2 x 2 factorial arrangement of treatments. diets were 1) steam-flaked ... | 1997 | 9436111 |
in vivo antioxidant activity of okara koji, a fermented okara, by aspergillus oryzae. | the antioxidants in okara koji (ok), an okara (oc) fermented by aspergillus oryzae, gamma-tocopherol, delta-tocopherol, genistin, daizein, genistein, and 3-hydroxyanthranilic acid were identified by hplc. ok's extract with 80% methanol strongly inhibited linoleate peroxidation, much more than other ok's extracts with hexane or hot water. the methanol extract accelerated 12-hydroxyeicosatetraenoic acid formation in membrane lipids at 10(-3) concentration, but inhibited the formation at higher con ... | 1997 | 9438976 |
characteristics of antibody responses induced in mice by protein allergens. | whereas many foreign proteins are immunogenic, only a proportion is also allergenic, having the capacity to induce the quality of immune response necessary to support the production of ige antibody. we have demonstrated previously that intraperitoneal administration to mice of proteins such as ovalbumin (ova) or the industrial enzyme a. oryzae lipase, which possess significant allergenic potential, stimulates the production of both igg and ige antibody. identical exposure to bovine serum albumin ... | 1997 | 9449226 |
trisaccharide synthesis by glycosyl transfer from p-nitrophenyl beta-d-n-acetylgalactosaminide on to disaccharide acceptors catalysed by the beta-n-acetylhexosaminidase from aspergillus oryzae. | the beta-n-acetylhexosaminidase from aspergillus oryzae catalysed the transfer of beta-d-n-acetylgalactosaminyl residues from p-nitrophenyl beta-d-n-acetylglucosaminide on to disaccharide acceptors consisting of thioethyl glycosides of alpha-d-glc-(1-->4)-beta-d-glc, beta-d-glc-(1-->4)-beta-d-glc and beta-d-glc-(1-->6)-beta-d-glc. the principle of 'anomeric control' was exemplified by the results which showed that an alpha-linkage between the units of the acceptor favoured exclusively the format ... | 1997 | 9648256 |
regioselectivity of the enzymatic transgalactosidation of d- and l-xylose catalysed by beta-galactosidases. | the regioselectivity of enzymatic transgalactosidation depends on the source of the beta-galactosidase used. when the galactosyl acceptor only contains secondary hydroxyl groups, e.g., d- or l-xylose, it is possible to find an enzyme that catalyses preferentially the synthesis of any of the three regioisomers 4-, 3- and 2-o-beta-d-galactopyranosyl-d-xylose (1, 2 and 3, respectively) or 4-, 3- and 2-o-beta-d-galactopyranosyl-l-xylose (4, 5 and 6, respectively). enriched mixtures in 1, 2 or 3 were ... | 1997 | 9648257 |
development of a two-site enzyme-linked immunosorbent assay for alpha-amylase from aspergillus oryzae based on monoclonal antibodies. | a two-site monoclonal antibody elisa was developed to quantify the allergen asp o 2 (alpha-amylase from aspergillus oryzae). two mabs recognizing distinct epitopes were selected, enriched by in vitro production in a modular minifermenter and affinity-purified. the first antibody was bound to microtiter plates which were then incubated with samples containing the allergen. bound allergen was detected using a biotinylated second antibody and peroxidase-polymer-labelled streptavidin. the assay had ... | 1997 | 9502588 |
nucleotide sequence of an alternative glucoamylase-encoding gene (glab) expressed in solid-state culture of aspergillus oryzae. | the dna (glab) and a cdna-encoding glucoamylase produced in solid-state culture of aspergillus oryzae were cloned using oligodeoxyribonucleotide probes derived from internal amino acid sequences of the enzyme. comparison of the nucleotide sequences of a genomic dna fragment with its cdna showed the glab gene carried three exons interrupted by two introns and had an open reading frame encoding 493 aa residues. the 5'-flanking region had a tata box at nt -87 from the start codon and two putative c ... | 1998 | 9511753 |
overproduction of 1,2-alpha-mannosidase, a glycochain processing enzyme, by aspergillus oryzae. | a recombinant strain of aspergillus oryzae has been constructed in which 1,2-alpha-mannosidase, an intracellular glycochain processing enzyme with specificity toward 1,2-alpha-mannosidic linkages, has been overexpressed. for the construction, the n-terminal signal-encoding sequence of the 1,2-alpha-mannosidase gene (msdc) from penicillium citrinum was replaced with that of the aspergillopepsin i signal, and the fused gene was inserted between amyb promoter-terminator elements in the expression p ... | 1998 | 9532788 |
hydrolysis of a slow cyclic thiophosphate substrate of rnase t1 analyzed by time-resolved crystallography. | here we present a time-resolved crystallographic analysis of the hydrolysis of exo (sp) guanosine 2',3'-cyclophosphorothioate by rnase t1. the use of a slow substrate and fast crystallization methods made it possible to perform the study with conventional data-collection techniques. the results support the idea that the hydrolysis reaction proceeds through a mechanism that is the inverse of the transesterification reaction. in addition, the structures provide an explanation for the differential ... | 1998 | 9546218 |
characterization of recombinant rhamnogalacturonan alpha-l-rhamnopyranosyl-(1,4)-alpha-d-galactopyranosyluronide lyase from aspergillus aculeatus. an enzyme that fragments rhamnogalacturonan i regions of pectin. | the four major oligomeric reaction products from saponified modified hairy regions (mhr-s) from apple, produced by recombinant rhamnogalacturonan (rg) alpha-l-rhamnopyranosyl-(1, 4)-alpha-d-galactopyranosyluronide lyase (rrg-lyase) from aspergillus aculeatus, were isolated and characterized by 1h-nuclear magnetic resonance spectroscopy. they contain an alternating rg backbone with a degree of polymerization of 4, 6, 8, and 10 and with an alpha-delta-(4,5)-unsaturated d-galactopyranosyluronic aci ... | 1998 | 9576783 |
[regulation of gene expression in aspergilli]. | the aspergillus oryzae taka-amylase a (taa) gene has been used as a model gene to characterize the regulatory mechanisms of gene expression in aspergilli. taa gene contained a typical eukaryotic promoter with a tata box and putative regulatory elements such as a ccaat sequence in its 5'-noncoding region. a nuclear protein designated ancp bound to the ccaat sequence. replacement of the ccaat sequence with cgtaa was found to abolish the binding of ancp and to have an inhibitory effect on taa promo ... | 1998 | 9580033 |
purification and characterization of triacylglycerol lipase from aspergillus oryzae. | triacylglycerol lipase (l3) was purified from aspergillus oryzae rib128 by ammonium sulfate fractionation, acetone precipitation, anion-exchange chromatography, and gel filtration. the purified enzyme was formed from a glycoprotein and a monomeric protein with molecular masses of 25 and 29 kda, by sds-page and gel filtration, respectively. the optimum ph at 40 degrees c was 5.5 and the optimum temperature at ph 5.5 was 40 degrees c. the enzyme was stable between a ph range of 4.0-7.5 at 30 degre ... | 1998 | 9614707 |
xylooligosaccharide production by aspergillus oryzae 13 immobilized on a nonwoven fabric. | immobilized mycelia were screened for xylooligosaccharide production from xylan, and 20 strains of aspergillus oryzae were selected. for its high activity and operational stability of xylooligosaccharides formation, immobilized a. oryzae 13 was selected for further examination. batch production of xylooligosaccharides from xylan by the immobilized mycelia was repeated a total of 4 times. | 1998 | 9614714 |
increase in milk yield of commercial dairy herds fed a microbial and enzyme supplement. | a microbial and enzyme supplement fed at 21.2 g/d per cow to 46 virginia dairy herds increased the milk yield of 31 herds (17 significantly) and decreased the milk yield of 15 herds (7 significantly). effects of season were important but consistent with overall results. herds began receiving the supplement, which contained dried fermentation products of aspergillus oryzae, bacillus subtilis, lactobacillus acidophilus, and yeast culture, midway between the first and second monthly dairy herd impr ... | 1998 | 9621238 |
alkaline serine proteinase: a major allergen of aspergillus oryzae and its cross-reactivity with penicillium citrinum. | aspergillus species are common indoor airborne fungi and have been considered as causative agents of human allergic disorders. however, allergens of different aspergillus species have not been effectively characterized. the object of this study was to identify and characterize ige-binding components of aspergillus oryzae. | 1998 | 9623506 |
polygalacturonase gene expression in ripe melon fruit supports a role for polygalacturonase in ripening-associated pectin disassembly. | ripening-associated pectin disassembly in melon is characterized by a decrease in molecular mass and an increase in the solubilization of polyuronide, modifications that in other fruit have been attributed to the activity of polygalacturonase (pg). although it has been reported that pg activity is absent during melon fruit ripening, a mechanism for pg-independent pectin disassembly has not been positively identified. here we provide evidence that pectin disassembly in melon (cucumis melo) may be ... | 1998 | 9625689 |
chaperonin groe-facilitated refolding of disulfide-bonded and reduced taka-amylase a from aspergillus oryzae. | the refolding characteristics of taka-amylase a (taa) from aspergillus oryzae in the presence of the chaperonin groe were studied in terms of activity and fluorescence. disulfide-bonded (intact) taa and non-disulfide-bonded (reduced) taa were unfolded in guanidine hydrochloride and refolded by dilution into buffer containing groe. the intermediates of both intact and reduced enzymes were trapped by groel in the absence of nucleotide. upon addition of nucleotides such as atp, adp, ctp or utp, the ... | 1998 | 9930680 |
localization of a phosphatidylglycerol/phosphatidylinositol transfer protein in aspergillus oryzae. | the subcellular localization of the phosphatidylglycerol/phosphatidylinositol transfer protein (pg/pi-tp) of aspergillus oryzae was investigated using western blot analysis of the cell protein extracts, a cellular membrane fractionation technique, and transmission electron microscopy. the pg/pi-tp, as detected by western blot analysis with a specific immune serum, was found to be mainly cytoplasmic and partly associated with intracellular membranes. a fractionation experiment was conducted after ... | 1998 | 9933913 |
ben1 and zen1 cdnas encoding s1-type dnases that are associated with programmed cell death in plants. | we describe the isolation and characterization of cdnas encoding two dnases that may be involved in the programmed death of plant cells: a 35-kda nuclease of barley, which had been reported to be secreted from the aleurone layer into the endosperm during germination and may be responsible for the digestion of nuclear dna in the course of degradation (cell death) of endosperm, and a 43-kda nuclease of zinnia, which appears transiently in association with differentiation to tracheary elements and ... | 1998 | 9650576 |
effect of process conditions on recovery of protein activity after freezing and freeze-drying. | the objective of this research was to gain a better understanding of the degree to which recovery of activity of model proteins after freeze-drying can be maximized by manipulation of freeze-dry process conditions in the absence of protective solutes. catalase, beta-galactosidase and lactate dehydrogenase (ldh) were used as model proteins. all of the three proteins exhibited a concentration-dependent loss of activity after freezing, with significantly higher recovery at higher concentration. the ... | 1998 | 9653629 |
effect of n-linked glycosylation on secretion, activity, and stability of alpha-amylase from aspergillus oryzae. | the effect of n-linked glycosylation on secretion, activity, and stability of alpha-amylase from aspergillus oryzae grown as dispersed filaments was studied. in the presence of tunicamycin the fungus grew either as dispersed filaments or as one large pellet, whereas growth was as dispersed filaments in all control cultures. the presence of tunicamycin affected neither biomass, level of secreted alpha-amylase, nor total amount of secreted protein in cultures growing as dispersed filaments. in the ... | 1998 | 9662611 |
effect of aspergillus oryzae extract alone or in combination with antimicrobial compounds on ruminal bacteria. | the effect of an aspergillus oryzae fermentation extract on the growth rates of pure cultures of ruminal bacteria was determined. bacteria were grown in an anaerobic ruminal fluid and carbohydrate medium. a sterile filtrate made with 10% a. oryzae was added to the medium at 2 or 5% (vol/vol) to provide a final a. oryzae concentration of 2 or 5 mg/ml, respectively. the filtrate had no effect on the growth rates of 10 of the 19 ruminal bacteria tested; however, the filtrate increased the growth ra ... | 1998 | 9684165 |
production of amylases from rice by solid-state fermentation in a gas-solid spouted-bed bioreactor | a gas-solid spouted-bed bioreactor was developed to produce amylases from rice in solid-state fermentation by aspergillus oryzae. the spouted-bed bioreactor was developed to overcome many of the problems inherent to large-scale solid-state fermentation, including mass- and heat-transfer limitations in the conventional tray reactors and solids-handling difficulties seen in packed-bed bioreactors. the solid-state fermentation results from the tray-type reactor with surface aeration were poor becau ... | 1998 | 9694679 |
utilization of the tef1-alpha gene (tef1) promoter for expression of polygalacturonase genes, pgaa and pgab, in aspergillus oryzae. | for the development of an efficient gene expression system in a shoyu koji mold aspergillus oryzae kbn616, the tef1 gene, encoding translation-elongation factor 1 alpha, was cloned from the same strain and used for expression of polygalacturonase genes. the tef1 gene comprised 1647 bp with three introns. the tef1-alpha protein consisted of 460 amino acids possessing high identify to other fungal tef proteins. two nucleotide sequences homologous to the upstream activation sequence, characterized ... | 1998 | 9720204 |
aspergillus oryzae with and without a homolog of aflatoxin biosynthetic gene ver-1. | part of the nucleotide sequence of the ver-1 homolog in each of two strains of aspergillus oryzae, aspergillus sojae, and aspergillus flavus were compared with two homologs in aspergillus parasiticus. the homologs in a. oryzae and a. sojae (non-aflatoxin-producers) exhibited an extremely high degree (93.8-99.8% for a. oryzae, and 96.0-99.5% for a. sojae) of sequence identify with that of a. flavus and a. parasiticus. no two strains within the same species, except a. sojae, were identical. no seq ... | 1998 | 9720206 |
recognition of single-stranded dna by nuclease p1: high resolution crystal structures of complexes with substrate analogs. | the reaction mechanism of nuclease p1 from penicillium citrinum has been investigated using single-stranded dithiophosphorylated di-, tetra-, and hexanucleotides as substrate analogs. the complexes crystallize in tetragonal and orthorhombic space groups and have been solved by molecular replacement. the high resolution structures give a clear picture of base recognition by p1 nuclease at its two nucleotide-binding sites, especially the 1.8 a structure of a p1-tetranucleotide complex which can be ... | 1998 | 9726413 |
role of the regulatory gene area of aspergillus oryzae in nitrogen metabolism. | the area gene of aspergillus oryzae was cloned by cross-hybridization with the aspergillus nidulans area gene and was found to encode an 866-amino-acid protein that is very similar to other fungal nitrogen regulatory proteins. the a. oryzae area gene can complement a. nidulans area loss-of-function mutations. functional analyses indicated that the n-terminal region of the a. oryzae area protein was dispensable for function and revealed a probable acidic activation domain in the protein. c-termin ... | 1998 | 9726865 |
isolation of the gene and characterization of the enzymatic properties of a major exoglucanase of humicola grisea without a cellulose-binding domain. | an exoglucanase gene was cloned from a cellulolytic fungus, humicola grisea. dna sequencing of this gene, designated as exo1, revealed that it contained four introns in the coding region. the deduced amino acid sequence of exo1 was 451 amino acids in length and showed 57.7% identity with that of h. grisea cellobiohydrolase 1 (cbh1), but lacked the typical domain structures of a cellulose-binding domain and a hinge region. transcriptional analysis of the exo1 and cbh1 genes showed that the expres ... | 1998 | 9756616 |
purification, characterization, and substrate specificity of a novel highly glucose-tolerant beta-glucosidase from aspergillus oryzae. | aspergillus oryzae was found to secrete two distinct beta-glucosidases when it was grown in liquid culture on various substrates. the major form had a molecular mass of 130 kda and was highly inhibited by glucose. the minor form, which was induced most effectively on quercetin (3,3',4',5,7-pentahydroxyflavone)-rich medium, represented no more than 18% of total beta-glucosidase activity but exhibited a high tolerance to glucose inhibition. this highly glucose-tolerant beta-glucosidase (designated ... | 1998 | 9758774 |
regulation of aflr and its product, aflr, associated with aflatoxin biosynthesis. | we studied the role of the regulatory gene aflr and its product, aflr, in the biosynthesis of aflatoxin in aspergillus. western blot and enzyme-linked immunosorbent assay analyses revealed that aflatoxin b1 accumulation was directly related to aflr expression and was regulated by various environmental and nutritional conditions, including temperature, air supply, carbon source, nitrogen source, and zinc availability. expression of an aflatoxin biosynthetic pathway structural gene, omta, was regu ... | 1998 | 9758790 |
[quantification of inhaled exposure to alpha-amylase in 2 bakeries]. | baker's asthma and baker's rhinitis are among the most frequent occupational diseases. a major cause is the high exposure to flour dust in the workplace and to allergenic enzymes like alpha-amylase from aspergillus oryzae (allergen name: asp o 2). | 1998 | 9774870 |
the effect of saccharomyces cerevisiae and aspergillus oryzae on the digestion of the cell wall fraction of a mixed diet in defaunated and refaunated sheep rumen. | the objective of this study was to determine the effect of two probiotics, saccharomyces cerevisiae (sc) and aspergillus oryzae (ao), without their culture medium, on the digestion of plant cell wall components in sheep that had been successively defaunated and refaunated. six sheep fitted with large rumen cannulae were used to study 1) defaunated sheep with no probiotic, 2) defaunated sheep with sc or ao, 3) refaunated sheep with no probiotic, 4) refaunated sheep with sc or ao. the apparent dig ... | 1998 | 9795984 |
overproduction of recombinant trichoderma reesei cellulases by aspergillus oryzae and their enzymatic properties. | we have established an expression system of trichoderma reesei cellulase genes using aspergillus oryzae as a host. in this system, the expression of t. reesei cellulase genes were regulated under the control of a. oryzae taka-amylase promoter and the cellulase genes were highly expressed when maltose was used as a main carbon source for inducer. the production of recombinant cellulases by a. oryzae transformants reached a maximum after 3-4 days of cultivation. in some cases, proteolysis of recom ... | 1998 | 9828459 |
microorganism inactivation using high-pressure generation in sealed vessels under sub-zero temperature. | in order to test the possibility of utilizing high pressure in bioscience and biotechnology, a simple method for high-pressure generation and its use for microbial inactivation have been studied. when a pressure vessel was filled with water, sealed tightly and cooled to sub-zero temperatures, high pressure was generated in the vessel. the pressure generation was 60 mpa at -5 degrees c, 103 mpa at -10 degrees c, and 140 mpa at -15 degrees c, -20 degrees c, and -22 degrees c. the high pressure gen ... | 1998 | 9830091 |
improvement of promoter activity by the introduction of multiple copies of the conserved region iii sequence, involved in the efficient expression of aspergillus oryzae amylase-encoding genes. | the role of the conserved sequence region iii in the promoter regions of the amylase-encoding genes amyb, glaa and agda of aspergillus oryzae was examined. introduction of multiple copies of the region iii fragment into the agda promoter resulted in a significant increase in promoter activity at the transcriptional level. this result suggests that the fragment comprising region iii consists of one or more cis-acting sequence(s). moreover, expression of the agda gene under the control of the impr ... | 1998 | 9830097 |
characterization of the prolyl dipeptidyl peptidase gene (dppiv) from the koji mold aspergillus oryzae. | the koji mold aspergillus oryzae secretes a prolyl dipeptidyl peptidase (dppiv) when the fungus is cultivated in a medium containing wheat gluten as the sole nitrogen and carbon source (mmwg). we cloned and sequenced the dppiv gene from an a. oryzae library by using the a. fumigatus dppiv gene as a probe. reverse transcriptase pcr experiments showed that the a. oryzae dppiv gene consists of two exons, the first of which is only 6 bp long. the gene encodes an 87.2-kda polypeptide chain which is s ... | 1998 | 9835566 |
biodegradation of ornithine-containing polylysine hydrogels. | the degradation of the cross-linked cationic poly(amino acid)-glutaraldehyde (ga) hydrogels by two kinds of proteolytic enzymes, trypsin and aspergillus protease type xxiii, and by seven species of soil filamentous fungi has been investigated using homo- and copolypeptides of lysine (lys) and ornithine (orn). trypsin degraded the hydrogels prepared from poly(lys) and copoly(lys orn)s but not poly(orn), while aspergillus protease degraded all of them. degradation time of hydrogels by the two prot ... | 1998 | 9855186 |
cloning of a thermostable ascorbate oxidase gene from acremonium sp. hi-25 and modification of the azide sensitivity of the enzyme by site-directed mutagenesis. | a gene encoding a thermostable ascorbate oxidase (asom) was cloned from acremonium sp. hi-25 and sequenced. the gene comprised 1709 bp and was interrupted by a single intron of 57 bp. asom consisted of 551 amino acids including a signal peptide with a molecular mass of 61200, and contained four histidine-rich regions with high sequence homology to the corresponding regions of other multicopper oxidases. the asom gene was expressed in aspergillus nidulans under the aspergillus oryzae taka-amylase ... | 1998 | 9858779 |
repression of the expression of genes encoding xylanolytic enzymes in aspergillus oryzae by introduction of multiple copies of the xynf1 promoter. | a xylanase gene, xynf1, was cloned and characterized from a shoyu koji mould aspergillus oryzae kbn616. the xynf1 gene was found to be comprised of 1484 bp with ten introns. the deduced amino acid sequence encodes a protein consisting of 327 amino acids (35,402 da) which is very similar to the fungal family f xylanases such as aspergillus nidulans xlnc, aspergillus kawachii xyna and penicillium chrysogenum xylp. the intron/exon organization of xynf1 is very similar to that of the fungal family f ... | 1998 | 9866173 |
transcript of a homolog of aflr, a regulatory gene for aflatoxin synthesis in aspergillus parasiticus, was not detected in aspergillus oryzae strains. | some strains of aspergillus oryzae were shown to have homologs of aflr, a regulatory gene for aflatoxin synthesis in aspergillus parasiticus. transcription of an aflr homolog was examined in six strains of a. oryzae having the homologs, using polymerase chain reaction (pcr) coupled with reverse transcription. no pcr product was obtained when the rna prepared from the a. oryzae strains cultivated under aflatoxin-producing condition was used as template for amplification of the aflr cdna. by contr ... | 1998 | 9868774 |
hydrolase-catalyzed beta-mannosylations. | transmannosylations catalysed by beta-mannosidase from snail viscera or beta-galactosidase from aspergillus oryzae were accomplished with 4-nitrophenyl beta-d-mannopyranoside as donor substrate. with suitable hydrophobic acceptor molecules preferentially beta1-4-linked disaccharides were obtained. the activities of both glycosidases in buffer cosolvent mixtures were determined, and conditions for their immobilization were elaborated and optimized. a model of the enzymic transfer mechanism is sug ... | 1998 | 9870351 |
action of trichoderma reesei and aspergillus oryzae esterases in the deacetylation of hemicelluloses. | xylans and mannans contain different esterified substituents such as acetyl, feruloyl and p-coumaroyl side groups. the functions of hemicellulose-deacetylating esterases of trichoderma reesei and aspergillus oryzae are discussed in this paper. both fungi produce multiple esterases and two different esterases were isolated from both t. reesei and a. oryzae. the enzymes differed significantly in their substrate specificities. acetyl xylan esterase of t. reesei was highly active on polymeric xylan ... | 1998 | 9477552 |
construction of a low-serine-type-carboxypeptidase-producing mutant of aspergillus oryzae by the expression of antisense rna and its use as a host for heterologous protein secretion. | using an antisense control strategy, we isolated an aspergillus oryzae mutant that produced low levels of carboxypeptidases (cpases). the mutant tfc-1 expressed the antisense rna of the structural gene of cpase o and showed about 30% of the cpase activity in the parent strain. gel filtration analysis indicated that this mutant decreased the cpase activities not only of cpase o but also of cpase o-1 and o-2. this result indicated that the antisense rna was able to control the expression of the cp ... | 1998 | 9487708 |
metabolic engineering: techniques for analysis of targets for genetic manipulations. | metabolic engineering has been defined as the purposeful modification of intermediary metabolism using recombinant dna techniques. with this definition metabolic engineering includes: (1) inserting new pathways in microorganisms with the aim of producing novel metabolites, e.g., production of polyketides by streptomyces; (2) production of heterologous peptides, e.g., production of human insulin, erythropoitin, and tpa; and (3) improvement of both new and existing processes, e.g., production of a ... | 1998 | 10191381 |
13c tracer experiments and metabolite balancing for metabolic flux analysis: comparing two approaches | conventional metabolic flux analysis uses the information gained from determination of measurable fluxes and a steady-state assumption for intracellular metabolites to calculate the metabolic fluxes in a given metabolic network. the determination of intracellular fluxes depends heavily on the correctness of the assumed stoichiometry including the presence of all reactions with a noticeable impact on the model metabolite balances. determination of fluxes in complex metabolic networks often requir ... | 1998 | 10191397 |
successful treatment of severe atopic dermatitis-complicated cataract and male infertility with a natural product antioxidant. | there has been a recent dramatic change in the features of atopic dermatitis and male infertility, including a marked increased prevalence of severe and treatment-resistant atopic dermatitis; an increase in severe atopic dermatitis complicated by cataracts, especially in urban and industrial areas; and an increase in the number of infertile men with poor sperm motility. previously we have attributed these changes to the increased free radicals produced by environmental toxicity. we have reported ... | 1998 | 9638503 |
growth and product formation of aspergillus oryzae during submerged cultivations: verification of a morphologically structured model using fluorescent probes. | a morphologically structured model is well suited for obtaining a good description of growth and product formation of filamentous fungi for use in a process model, for example. this article describes a new morphologically structured model and its application to an alpha-amylase producing strain of aspergillus oryzae. the model is based on a division of the fungal hyphae into three different regions: an extension zone, representing the tips of the hyphae; an active region, which is responsible fo ... | 1998 | 10099209 |
on-line study of fungal morphology during submerged growth in a small flow-through cell. | a flow-through cell is designed to measure the growth kinetics of hyphae of aspergillus oryzae grown submerged in a well controlled environment. the different stages of the growth process are characterized, from the spore to the fully developed hyphal element with up to 60 branches and a total length lt up to 10,000 micrometer. spore swelling is found to occur without change in the form of the spore (circularity index constant at about 1.06) and the spore volume probably increases exponentially. ... | 1998 | 10099291 |
morphological change and enhanced pigment production of monascus when cocultured with saccharomyces cerevisiae or aspergillus oryzae | when a monascus isolate, a producer of monascus pigments, was cocultured with either saccharomyces cerevisiae or aspergillus oryzae in a solid sucrose medium, there were significant morphological changes in monascus culture. cocultures exhibited cell mass increases of 2 times and pigment yield increases of 30 to 40 times compared to monocultures of monascus. however, enhanced cell growth, an increase in pigment production, and morphological change did not occur in coculture with bacillus cereus. ... | 1998 | 10099374 |
enzymatic transformations in supersaturated substrate solutions: i. a general study with glycosidases. | the results of an initial study of enzymatic catalysis in metastable supersaturated solutions of carbohydrates are presented. it has been shown that such solutions, formed in the presence of small amounts of water and alcohol as plasticizers, are sufficiently stable under ambient conditions to enable enzymatic transformations of substrates. a partial phase diagram for a system consisting of glucose, water, and (poly)ethylene glycol was constructed to identify the regions which are most suitable ... | 1998 | 10099420 |
evolutionary relationships among aspergillus oryzae and related species based on the sequences of 18s rrna genes and internal transcribed spacers. | 1998 | 12501432 | |
preliminary studies on production of extracellular lipase by an isolate from soil : aspergillus flavus oryzae. | in our routine screening programme, using agar diffusion assay method, lipolytic activity was detected around a colony of a fungus. the fungus was isolated from a soil sample which was brought from a location near oil-mill. this lipolytic fungus was then identified to belong to aspergillus flavus oryzae. a medium was then formulated and optimized which would not only support good growth but also would yield good extracellular lipolytic activity. it was observed that a conventional carbohydrate-p ... | 1998 | 16961201 |
cysteine protease inhibitors produced by the industrial koji mold, aspergillus oryzae o-1018. | aspergillus oryzae o-1018 (ferm p-15834) separated from industrial koji for brewing sake was found to produce five papain-inhibitory compounds in the culture supernatant. the five isolated inhibitors were named cpi-1 to cpi-5, and their structures were elucidated by spectroscopic analyses and chemical degradation. we determined the structures of cpi-2, cpi-3 and cpi-4 as 4-amino-1-[[n- [(2s, 3s)-3-trans-carboxyoxiran-2-carbonyl]-l-isoleucyl] amino]butane, 5-amino-1-[[n-[(2s, 3s)-3-trans-carboxyo ... | 1998 | 27392587 |
transglycosylation of thiamin by fungal β-n-acetylhexosaminidases. | a new thiamin glycoside, e.g., thiamin β-d-2-deoxy-2-acetamidoglucopyranoside was prepared by transglycosylation by β-n-acetylhexosaminidase from aspergillus oryzae and characterized spectrally. series of other fungal β-n-acetyl-hexosaminidases from a. awamori, a. tamari, a. terreus, and penicillium oxalicum were shown to be able to synthesize the same compound in lower yields. | 1998 | 27392399 |
purification and characterization of isoamyl alcohol oxidase ("mureka"-forming enzyme). | isoamyl alcohol oxidase (iaaod) was purified to apparent homogeneity on sds-page from ultrafiltration (uf) concentrated sake. iaaod was a glycoprotein, a monomeric protein with an apparent molecular mass of 73 and 87 kda, by sds-page and gel filtration on hplc, respectively. iaaod showed high substrate specificity toward c5 branched-chain alkyl alcohol (isoamyl alcohol), and no activity toward shorter (c1-c4) or longer (c7-c10) alkyl alcohols tested. iaaod was stable between ph 3.0-6.0 at 25°c. ... | 1999 | 27380232 |
dry fermented sausages made with a protease from aspergillus oryzae and/or a starter culture. | sausages with a starter culture (lactobacillus plantarum + staphylococcus carnosus), a protease (fungal protease from solvay enzymes) and both enzyme/starter were produced from the same raw matter under the same conditions for 15 days of ripening. the lowest ph values were found in fungal/starter sausages. significantly higher amounts of free amino acids (faa) were seen in sausages containing enzyme from the 3rd day of ripening, whereas amino acids from peptides (paa) were significantly higher o ... | 1999 | 22062704 |
cloning and functional analysis of the aspergillus oryzae conidiation regulator gene brla by its disruption and misscheduled expression. | we cloned the brla gene from aspergillus oryzae genomic dna using the a. nidulans brla gene as a probe. a 3.1-kb ecori-bali genomic dna fragment was cloned and sequenced. the deduced amino acid sequence revealed 70% identity with a. nidulans brla and contained two c2h2 zinc finger motifs in its carboxyl terminus, and the promoter sequence contained a 43-bp highly conserved region, indicating that the cloned gene was an a. oryzae homologue of a. nidulans brla. disruption of the brla gene by homol ... | 1999 | 16232494 |
properties of the peptides liberated from rice protein in sokujo-moto. | in the supernatant of sokujo-moto, a high level of acid carboxypeptidase (acp) activity and a large amount of peptides were observed, however, the amount of free amino acids liberated was small. in order to determine why these peptides were not hydrolyzed to any significant degree by the acp, the properties of the peptides in sokujo-moto were investigated in this study. peptides were fractionated from sokujo-moto by ion exchange column chromatography. acp purified from rice-koji (rice overgrown ... | 1999 | 16232611 |
aspergillus oryzae palbory encodes a calpain-like protease: homology to emericella nidulans palb and conservation of functional regions. | we have cloned and sequenced genomic dna of aspergillus oryzae palbory, orthologue of emericella nidulans palb, which encodes a calpain-like protease modulating a signal transduction pathway during alkaline adaptation. the deduced amino acid sequence of palbory is 70.0% identical to palb over its entire length. the regions with high similarity revealed possible domains important for their function. this is the first step towards understanding the alkaline adaptation mechanism of a. oryzae, which ... | 1999 | 16232641 |
cloning and characterization of the bipa gene encoding er chaperone bip from aspergillus oryzae. | we describe the cloning and characterization of the er localized chaperone (bip) encoding gene from the filamentous fungus aspergillus oryzae. the bip encoding gene, designated bipa, has three introns and encodes a protein with 672 amino acids, which has a high homology with various bip-like proteins. sequences resembling heat shock elements (hses) and unfolded protein response (upr) elements, as found in the saccharomyces cerevisiae kar2 promoter, are present in the 5' no coding region of the b ... | 1999 | 16232647 |
production and some properties of salt-tolerant beta-xylosidases from a shoyu koji mold, aspergillus oryzae in solid and liquid cultures. | beta-xylosidase production from a shoyu (soy sauce) koji mold, aspergillus oryzae hl15, cultured in solid and liquid media was examined and some properties of the enzymes were studied. three beta-xylosidases (xy11, xy12 and xy13) were easily extracted with 0.5% nacl from a solid medium and purified homogeneously on sds-page by chromatography. on the other hand, in a liquid medium, a. oryzae hl15 produced mainly cell-wall-bound beta-xylosidases which could not be extracted with 0.5% nacl or any d ... | 1999 | 16232648 |
production of alkaline protease by a genetically engineered aspergillus oryzae u1521. | the production of alkaline protease of aspergillus oryzae u1521 was examined in liquid culture. in a culture of defatted soybean only, it gave satisfactory enzyme yields at 584,000 u/g defatted soybean. when various carbohydrates were supplemented, enzyme production was significantly increased. an increase in production by lactose was the most marked. enrichment with casitone or casein increased productivity, but not cornsteep solid. media formulation (g/l) of defatted soybean 10, lactose 5, cas ... | 1999 | 12501379 |
[quantitative determination of 3-nitropropionic acid by hplc]. | a reversed-phase liquid chromatographic method to determine the concentration of 3-nitropropionic acid (3-npa) in the culture medium with aspergillus oryzae was developed. the culture medium samples were extracted with ethyl acetate. the analytic column was hypersil c18 and the mobile phase was ch3cn/kh2po4(0.02 mol/l, ph 3.0) (1/3, v/v). the sample was detected at uv210 nm. the detection limit of this method was at 10 micrograms/kg level and recoveries were 90.1%-98.0. | 1999 | 12712703 |
effects of agitation intensity on mycelial morphology and protein production in chemostat cultures of recombinant aspergillus oryzae. | the effects of agitation on fragmentation of a recombinant strain of aspergillus oryzae and its consequential effects on protein production have been investigated. constant mass, 5.3-l chemostat cultures at a dilution rate of 0.05 h-1 and a dissolved oxygen level of 75% air saturation, have been conducted at 550, 700, and 1000 rpm. these agitation speeds were chosen to cover a range of specific power inputs (2.2 to 12 kw m-3) from realistic industrial levels to much higher values. the use of a c ... | 1999 | 10099554 |
on-line study of growth kinetics of single hyphae of aspergillus oryzae in a flow-through cell. | using image analysis the growth kinetics of the single hyphae of the filamentous fungus aspergillus oryzae has been determined on-line in a flow-through cell at different glucose concentrations in the range from 26 mg l-1 to 20 g l-1. the tip extension rate of the individual hyphae can be described with saturation type kinetics with respect to the length of the hyphae. the maximum tip extension rate is constant for all hyphae measured at the same glucose concentration, whereas the saturation con ... | 1999 | 10099591 |
solid-state fermentation in rotating drum bioreactors: operating variables affect performance through their effects on transport phenomena. | aspergillus oryzae acm 4996 was grown on an artificial gel-based substrate and on steamed wheat bran during solid-state fermentations in 18.7 l rotating drum bioreactors. for gel fermentations fungal growth decreased as rotational speed increased, presumably due to increased shear. for wheat bran fermentations fungal growth improved under agitated compared to static culture conditions, due to superior heat and mass transfer. we conclude that the effects of operational variables on the performanc ... | 1999 | 10099618 |
site-directed mutagenesis of a possible type 1 copper ligand of bilirubin oxidase; a met467gln mutant shows stellacyanin-like properties. | in our previous paper, we reported a mutant of recombinant myrothecium verrucaria bilirubin oxidase, in which the met467 residue was replaced by gly [shimizu, a. et al. (1999) biochemistry 38, 3034-3042]. this mutant displayed a remarkable reduction in enzymatic activity and an evident decrease in the intensity of the absorption band around 600 nm (type 1 charge transfer transition). in this study, we report the preparation of three met467 mutants (met467gln, met467his, and met467arg) and charac ... | 1999 | 10101277 |
molecular cloning and expression of the novel fungal beta-glucosidase genes from humicola grisea and trichoderma reesei. | a novel fungal beta-glucosidase gene (bgl4) and its homologue (bgl2) were cloned from the cellulolytic fungi humicola grisea and trichoderma reesei, respectively. the deduced amino acid sequences of h. grisea bgl4 and t. reesei bgl2 comprise 476 and 466 amino acids, respectively, and share 73.1% identity. these beta-glucosidases show significant homology to plant beta-glucosidases belonging to the beta-glucosidase a (bga) family. both genes were expressed in aspergillus oryzae, and the recombina ... | 1999 | 10101286 |
fermentation extract effects on the morphology and metabolism of the rumen fungus neocallimastix frontalis eb188. | the effects of aspergillus oryzae fermentation extract, amaferm, on the rumen fungus neocallimastix frontalis eb188 were studied. the secretion of cellulase was increased by 67% and rhyzoid development was increased 3.8-fold in the presence of extract. strength of fungal response increased in a dose-dependent manner and demonstrated a positive correlation between cell surface area and enzyme secretion. above certain concentrations of extract, however, the development of the fungus and enzyme sec ... | 1999 | 10196744 |
targeted mutations in a trametes villosa laccase. axial perturbations of the t1 copper. | trametes villosa laccase was mutated on a tetrapeptide segment near the type 1 site. the mutations f463m and f463l were at the position corresponding to the type 1 copper axial methionine (m517) ligand in zucchini ascorbate oxidase. the mutations e460s and a461e were near the t1 copper site. the mutated trametes laccases were expressed in an aspergillus oryzae host and characterized. the e460s mutation failed to produce a transformant with meaningful expression. the f463l and a461e mutations did ... | 1999 | 10212209 |
molecular and enzymic properties of recombinant 1, 2-alpha-mannosidase from aspergillus saitoi overexpressed in aspergillus oryzae cells. | for the construction of an overexpression system of the intracellular 1,2-alpha-mannosidase (ec 3.2.1.113) gene (msds) from aspergillus saitoi (now designated aspergillus phoenicis), the n-terminal signal sequence of the gene was replaced with that of the aspergillopepsin i (ec 3.4.23.18) gene (apns) signal, one of the same strains as described previously. then the fused 1, 2-alpha-mannosidase gene (f-msds) was inserted into the noti site between p-no8142 and t-agda in the plasmid pnan 8142 (9.5 ... | 1999 | 10215597 |
identification of delta12-fatty acid desaturase from arachidonic acid-producing mortierella fungus by heterologous expression in the yeast saccharomyces cerevisiae and the fungus aspergillus oryzae. | based on the sequence information for the omega3-desaturase genes (from brassica napus and caenorhabditis elegans), which are involved in the desaturation of linoleic acid (delta9, delta12-18 : 2) to alpha-linolenic acid (delta9, delta12, delta15-18 : 3), a cdna was cloned from the filamentous fungal strain, mortierella alpina 1s-4, which is used industrially to produce arachidonic acid. homology analysis with protein databases revealed that the amino acid sequence showed 43.7% identity as the h ... | 1999 | 10215899 |
antioxidant activity of a medicine based on aspergillus oryzae nk koji measured by a modified t-butyl peroxyl radical scavenging assay. | a koji-based medicine composed of powder of aspergillus oryzae nk koji, dried yeast, and lactobacilli koji had high antioxidant activity measured by a modified t-butyl peroxyl radical scavenging assay. this activity was mainly derived from a. oryzae nk koji. digestion of koji-making grain germ medium with several commercial enzymes also increased antioxidant activity. by two weeks of oral administration of a. oryzae nk koji, the serum lipid peroxide levels elevated in stz-induced diabetic rats c ... | 1999 | 10227147 |
[oxidative injury and its defense system in vivo]. | we and other researchers verified that excessively produced free radicals by neutrophils induce various diseases such as behçet's disease, mcls, sle (neutrophil-stimulated lymphocytes), ra (synovial fluid neutrophils), crohn's disease, colitis ulcerosa, and dermatitis herpetiformis (dühring). recently, it was reported that environmental toxic agents including herbicides such as paraquat, insecticides, nitrogen oxide, and ultraviolet radiation produce free radicals. nitrogen oxide, a main product ... | 1999 | 10228384 |
stable high-copy-number integration of aspergillus oryzae alpha-amylase cdna in an industrial baker's yeast strain. | the aspergillus oryzae alpha-amylase cdna was placed under the control of the saccharomyces cerevisiae actin promoter (pact1) and introduced into the ribosomal dna locus of an industrial baker's yeast strain. to obtain a strain eligible for commercial use, we constructed an integrative cassette lacking bacterial dna sequences but containing the alpha-amylase cdna and ribosomal dna sequences to target the integration to this locus. high-copy-number integrants were obtained including a defective t ... | 1999 | 10356263 |
a cross-sectional survey of sensitization to aspergillus oryzae-derived lactase in pharmaceutical workers. | the presence of ige-mediated occupational respiratory sensitization to microbial enzymes has been well documented in a variety of industries. aspergillus oryzae -derived lactase is used as a dietary aid for patients with lactose intolerance. | 1999 | 10359899 |
production of recombinant der fi (a major mite allergen) by aspergillus oryzae. | der fi is a major mite allergen. to produce der fi by aspergillus oryzae, we placed a dna fragment encoding precursor-type recombinant der fi e(-1)k (reder fi e(-1) k), which had the c-terminal amino acid of the pro-sequence (glu) changed to lys, downstream of the glaa gene promoter and introduced it into aspergillus oryzae. in liquid culture, most of the reder fi e(-1)k produced by the transformants was degraded when culture was shaken vigorously. however, the degradation of reder fi e(-1)k was ... | 1999 | 10361683 |
fermentation of low-salt miso as affected by supplementation with ethanol. | steam-cooked soybeans and rice koji were combined (1:1, w/w), mixed with 5% (w/w) nacl and ground into a fine paste. samples (30 g) were deposited in nylon/polyethylene plastic bags and supplemented with 10 ml of aqueous ethanol solutions to give concentrations of 0, 2.5, 5, 7.5, 10, 15, 20, and 25% ethanol. mixtures were homogenized, sealed, and incubated at 28 degrees c for eight weeks. mold populations were less than 3 log10 cfu/g in all miso products after four weeks of fermentation. yeast p ... | 1999 | 10375131 |
molecular cloning and expression of the gene encoding a phospholipase a1 from aspergillus oryzae. | phospholipase a1 (pla1) is a hydrolytic enzyme that catalyzes removal of the acyl group from position 1 of lecithin to form lysolecithin. the genomic dna and cdna encoding pla1 from aspergillus oryzae were cloned with the mixed deoxyribonucleotide-primed polymerase chain reaction. the pla1 gene is composed of 1,056 bp and has four exons and three short introns (63, 54, and 51 bp). the deduced amino acid sequence of pla1 contained the n-terminal sequence of the mature pla1 analyzed by edman degra ... | 1999 | 10380624 |
alcoholysis reactions from starch with alpha-amylases. | the ability of alpha-amylases from different sources to carry out reactions of alcoholysis was studied using methanol as substrate. it was found that while the enzymes from aspergillus niger and aspergillus oryzae, two well-studied saccharifying amylases, are capable of alcoholysis reactions, the classical bacterial liquefying alpha-amylases from bacillus licheniformis and bacillus stearothermophilus are not. the effect of starch and methanol concentration, temperature and ph on the synthesis of ... | 1999 | 10386619 |
adapting protein solubility by glycosylation. n-glycosylation mutants of coprinus cinereus peroxidase in salt and organic solutions. | protein solubility is a fundamental parameter in biology and biotechnology. in the present study we have constructed and analyzed five mutants of coprinus cinereus peroxidase (cip) with 0, 1, 2, 4 and 6 n-glycosylation sites. all mutants contain man(x)(glcnac)(2) glycans. the peroxidase activity was the same for wild-type cip and all the glycosylation mutants when measured with the large substrate 2,2'-azino-bis(-3-ethylbenzthiazoline-6-sulfonic acid). the solubility of the five cip mutants show ... | 1999 | 10407143 |
treatment of melasma using kojic acid in a gel containing hydroquinone and glycolic acid. | melasma is difficult to clear. many agents have been used, such as hydroquinone, and glycolic acid and glycolic acid peels, kojic acid, a tyrosinase inhibitor in the fungus aspergilline oryzae. | 1999 | 10417583 |
purification, characterization, and heterologous expression in fusarium venenatum of a novel serine carboxypeptidase from aspergillus oryzae. | a novel serine carboxypeptidase (ec 3.4.16.1) was found in an aspergillus oryzae fermentation broth and was purified to homogeneity. this enzyme has a molecular weight of ca. 67,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and its specific activity is 21 u/mg for carbobenzoxy (z)-ala-glu at ph 4.5 and 25 degrees c. it has a ratio of bimolecular constants for z-ala-lys and z-ala-phe of 3.75. optimal enzyme activity occurs at ph 4 to 4.5 and 58 to 60 degrees c f ... | 1999 | 10427010 |
immobilization of enzymes on spongy polyvinyl alcohol cryogels: the example of beta-galactosidase from aspergillus oryzae. | the catalytic properties of a beta-galactosidase from aspergillus oryzae, entrapped into a spongy polyvinyl alcohol cryogel, were studied. this polymeric matrix was selected because of its mild conditions of preparation and its stability, biocompatibility, structural strength and diffusive properties. the enzyme was entrapped, in high percentage, into cryogel sponges and its activity and kinetic parameters were determined and compared with those of the free enzyme, using as substrates o-nitrophe ... | 1999 | 10434188 |
kinetics of alpha-amylase secretion in aspergillus oryzae. | pulse and pulse-chase experiments have been performed to study l-[(35)s] methionine incorporation and protein secretion kinetics in aspergillus oryzae. pulse experiments confirmed the mechanism of methionine uptake reported previously for penicillium chrysogenum (benko et al., 1967). pulse-chase experiments were carried out to investigate the alpha-amylase secretion kinetics in a. oryzae. no unglycosylated alpha-amylase was detected neither intracellularly nor extracellularly demonstrating that ... | 1999 | 10440673 |
aspzincin, a family of metalloendopeptidases with a new zinc-binding motif. identification of new zinc-binding sites (his(128), his(132), and asp(164)) and three catalytically crucial residues (glu(129), asp(143), and tyr(106)) of deuterolysin from aspergillus oryzae by site-directed mutagenesis. | deuterolysin (ec 3.4.24.39; formerly designated as neutral proteinase ii) from aspergillus oryzae, which contains 1 g atom of zinc/mol of enzyme, is a single chain of 177 amino acid residues, includes three disulfide bonds, and has a molecular mass of 19,018 da. active-site determination of the recombinant enzyme expressed in escherichia coli was performed by site-directed mutagenesis. substitutions of his(128) and his(132) with arg, of glu(129) with gln or asp, of asp(143) with asn or glu, of a ... | 1999 | 10446194 |
crystal structure of a ribonuclease from the seeds of bitter gourd (momordica charantia) at 1.75 a resolution. | the ribonuclease mc1 (rnase mc1) from seeds of bitter gourd (momordica charantia) consists of 190 amino acid residues with four disulfide bridges and belongs to the rnase t(2) family, including fungal rnases typified by rnase rh from rhizopus niveus and rnase t(2) from aspergillus oryzae. the crystal structure of rnase mc1 has been determined at 1.75 a resolution with an r-factor of 19.7% using the single isomorphous replacement method. rnase mc1 structurally belongs to the (alpha+beta) class of ... | 1999 | 10446375 |
exposure-response relations of alpha-amylase sensitisation in british bakeries and flour mills. | to describe the levels of exposure to fungal alpha-amylase in british bakeries and flour mills, and to describe the relation between exposure to alpha-amylase and sensitisation to fungal alpha-amylase. | 1999 | 10448329 |
the tama protein fused to a dna-binding domain can recruit area, the major nitrogen regulatory protein, to activate gene expression in aspergillus nidulans. | the area gene of aspergillus nidulans encodes a gata zinc finger transcription factor that activates the expression of a large number of genes subject to nitrogen metabolite repression. the amount and activity of the area protein under different nitrogen conditions is modulated by transcriptional, post-transcriptional, and post-translational controls. one of these controls of area activity has been proposed to involve the nmra protein interacting with the dna-binding domain and the extreme c ter ... | 1999 | 10471703 |
identification of an nadh-cytochrome b(5) reductase gene from an arachidonic acid-producing fungus, mortierella alpina 1s-4, by sequencing of the encoding cdna and heterologous expression in a fungus, aspergillus oryzae. | based on the sequence information for bovine and yeast nadh-cytochrome b(5) reductases (cbrs), a dna fragment was cloned from mortierella alpina 1s-4 after pcr amplification. this fragment was used as a probe to isolate a cdna clone with an open reading frame encoding 298 amino acid residues which show marked sequence similarity to cbrs from other sources, such as yeast (saccharomyces cerevisiae), bovine, human, and rat cbrs. these results suggested that this cdna is a cbr gene. the results of a ... | 1999 | 10473389 |
heterologous expression and product identification of colletotrichum lagenarium polyketide synthase encoded by the pks1 gene involved in melanin biosynthesis. | the colletotrichum lagenarium pks1 gene was expressed in the heterologous fungal host, aspergillus oryzae, under the starch-inducible alpha-amylase promoter to identify the direct product of polyketide synthase (pks) encoded by the pks1 gene. the main compound produced by an a. oryzae transformant was isolated and characterized to be 1,3,6,8-tetrahydroxynaphthalene (t4hn) as its tetraacetate. since the pks1 gene was cloned from c. lagenarium to complement the nonmelanizing albino mutant, t4hn wa ... | 1999 | 10501004 |
new potent antioxidative o-dihydroxyisoflavones in fermented japanese soybean products. | a potent antioxidative 6-hydroxydaidzein (6-ohd) was newly isolated from soybean koji fermented with aspergillus oryzae. 6-ohd, in addition to 8-hydroxydaidzein and 8-hydroxygenistein, were found to be present in various fermented soybean products, including their koji. considering that these o-dihydroxyisoflavones had strong antioxidative activities, they may contribute to protecting from oxidative deterioration during the processing of fermented soybean products. | 1999 | 10540753 |
molecular characterization of laccase genes from the basidiomycete coprinus cinereus and heterologous expression of the laccase lcc1. | a laccase from coprinus cinereus is active at alkaline ph, an essential property for some potential applications. we cloned and sequenced three laccase genes (lcc1, lcc2, and lcc3) from the ink cap basidiomycete c. cinereus. the lcc1 gene contained 7 introns, while both lcc2 and lcc3 contained 13 introns. the predicted mature proteins (lcc1 to lcc3) are 58 to 80% identical at the amino acid level. the predicted lcc1 contains a 23-amino-acid c-terminal extension rich in arginine and lysine, sugge ... | 1999 | 10543807 |
cloning and characterization of the o-methyltransferase i gene (dmta) from aspergillus parasiticus associated with the conversions of demethylsterigmatocystin to sterigmatocystin and dihydrodemethylsterigmatocystin to dihydrosterigmatocystin in aflatoxin biosynthesis. | o-methyltransferase i catalyzes both the conversion of demethylsterigmatocystin to sterigmatocystin and the conversion of dihydrodemethylsterigmatocystin to dihydrosterigmatocystin during aflatoxin biosynthesis. in this study, both genomic cloning and cdna cloning of the gene encoding o-methyltransferase i were accomplished by using pcr strategies, such as conventional pcr based on the n-terminal amino acid sequence of the purified enzyme, 5' and 3' rapid amplification of cdna ends pcr, and ther ... | 1999 | 10543813 |
effect of branch frequency in aspergillus oryzae on protein secretion and culture viscosity. | highly branched mutants of two strains of aspergillus oryzae (ifo4177, which produces alpha-amylase, and a transformant of ifo4177 [amg#13], which produces heterologous glucoamylase in addition to alpha-amylase) were generated by uv or nitrous acid mutagenesis. four mutants of the parental strain (ifo4177), which were 10 to 50% more branched than the parental strain, were studied in stirred batch culture and no differences were observed in either the amount or the rate of enzyme production. five ... | 1999 | 10550770 |
a modified chemical procedure for rapid determination of glucosamine and its application for estimation of mold growth in peanut kernels and koji. | one-step hydrolysis of chitin to release glucosamine for quantitation was achieved by combining a chitin-containing sample (10-200 mg of sample size) in a test tube with 1 ml of 10 m hcl followed by vacuum treatment for 10 min, incubation at 28 degrees c for 30 min, replenishment with 3 ml of deionized water, nitrogen flushing, screw capping, and heat treatment at 140 degrees c for 60 min. a phosphate buffer solution (ph 12.5, 0.2 m) was effective in ph stabilization and enhancing colorimetric d ... | 1999 | 10552485 |
characteristics and use of okara, the soybean residue from soy milk production--a review. | large quantities of okara produced annually pose a significant disposal problem. it contains mostly crude fiber composed of cellulose, hemicellulose, and lignin, about 25% protein, 10-15% oil, but little starch or simple carbohydrates. it is a suitable dietary additive in biscuits and snacks because it reduces calorie intake and increases dietary fiber. the high-quality protein fraction has good water holding and emulsifying qualities and contains a peptide with anti-hypertension effects. the pe ... | 1999 | 10563901 |
delta6-fatty acid desaturase from an arachidonic acid-producing mortierella fungus. gene cloning and its heterologous expression in a fungus, aspergillus. | a dna fragment was cloned from the fungal strain, mortierella alpina 1s-4 (which is used industrially to produce arachidonic acid), after pcr amplification with oligonucleotide primers designed based on the sequence information for delta6-desaturase genes (from borage and caenorhabditis elegans), which are involved in the desaturation of linoleic acid (delta9, delta12-18:2) to gamma-linolenic acid (delta6, delta9, delta12-18:3). this fragment was used as a probe to isolate a cdna clone with an o ... | 1999 | 10570972 |