TitleAbstractYear(sorted ascending)
high-efficiency removal of phytic acid in soy meal using two-stage temperature-induced aspergillus oryzae solid-state fermentation.phytic acid of soy meal (sm) could influence protein and important mineral digestion of monogastric animals. aspergillus oryzae (atcc 9362) solid-state fermentation was applied to degrade phytic acid in sm. two-stage temperature fermentation protocol was investigated to increase the degradation rate. the first stage was to maximize phytase production and the second stage was to realize the maximum enzymatic degradation.201423633040
allergens causing occupational asthma: an evidence-based evaluation of the literature.the aim of this work is to provide an evidence-based evaluation and overview of causative substances in order to improve disease management.201423595938
fed-batch synthesis of galacto-oligosaccharides with aspergillus oryzae β-galactosidase using optimal control strategy.fed-batch synthesis of galacto-oligosaccharides (gos) from lactose with β-galactosidase from aspergillus oryzae was evaluated experimentally and reaction yield was maximized via optimal control technique. the optimal lactose and enzyme feed flow rate profiles were determined using a model for gos synthesis previously reported by the authors. experimentally it was found that fed-batch synthesis allowed an increase on the maximum total gos concentration from 115 (batch synthesis) to 218 g l(-1) as ...201424167086
purification and partial characterization of nad aminohydrolase from aspergillus oryzae nrrl447.aspergillus oryzae aminohydrolase free acid phosphodiesterase catalyzes nicotinamide adenine dinucleotide to deamino-nad and ammonia. the enzyme was purified to homogeneity by a combination of acetone precipitation, anion exchange chromatography and gel filtration chromatography. the enzyme was purified 230.5 fold. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme showed a single protein band of mw 94 kda. the enzyme displayed maximum activity at ph 5 and 40 °c wit ...201424158390
improvement of kojic acid production in aspergillus oryzae b008 mutant strain and its uses in fermentation of concentrated corn stalk hydrolysate.a strain designated m866, producing kojic acid with a high yield, was obtained by combining induced mutation using ion beam implantation and ethyl methane sulfonate treatment of a wild type strain of aspergillus oryzae b008. the amount of kojic acid produced by the strain m866 in a shaking flask was 40.2 g/l from 100 g/l of glucose, which was 1.7 times higher than that produced by wild strain (23.58 g/l). when the mixture of glucose and xylose was used as carbon source, the resulting kojic acid ...201424170020
use of spent osmotic solutions for the production of fructooligosaccharides by aspergillus oryzae the food industry, osmotic dehydration can be an important stage to obtain partially dry foodstuffs. however, the remaining spent osmotic solution at the end of the process could become a waste with an important environmental impact due to the large amount of organic compounds that it might contain. since one of the most important osmotic agents used in osmotic dehydration is sucrose, this spent osmotic solution could be used to be biotransformed to produce fructooligosaccharides by a fructos ...201423744119
galactooligosaccharides formation during enzymatic hydrolysis of lactose: towards a prebiotic-enriched milk.the formation of galactooligosaccharides (gos) in skim milk during treatment with several commercial β-galactosidases (bacillus circulans, kluyveromyces lactis and aspergillus oryzae) was analysed in detail, at 4 and 40°c. the maximum gos concentration was obtained at a lactose conversion of approximately 40-50% with b. circulans and a. oryzae β-galactosidases, and at 95% lactose depletion for k. lactis β-galactosidase. using an enzyme dosage of 0.1% (v/v), the maximum gos concentration with k. ...201424128493
kinetic characterization of galacto-oligosaccharide (gos) synthesis by three commercially important β-galactosidases.many β-galactosidases show large differences in galacto-oligosaccharide (gos) production and lactose hydrolysis. in this study, a kinetic model is developed in which the effect of lactose, glucose, galactose, and oligosaccharides on the onpg converting activity of various β-galactosidases is quantified. the use of onpg as a competing substrate to lactose yields more information than can be obtained by examining only the conversion of lactose itself. the reaction rate with lactose or oligosacchar ...201424124064
a promiscuous prenyltransferase from aspergillus oryzae catalyses c-prenylations of hydroxynaphthalenes in the presence of different prenyl donors.prenyltransferases of the dimethylallyltryptophan synthase (dmats) superfamily are involved in the biosynthesis of secondary metabolites and show broad substrate specificity towards their aromatic substrates with a high regioselectivity for the prenylation reactions. most members of this superfamily accepted as prenyl donor exclusively dimethylallyl diphosphate (dmapp). one enzyme, anapt from neosartorya fischeri, was reported recently to use both dmapp and geranyl diphosphate (gpp) as prenyl do ...201424430210
analyzing the relation between the microbial diversity of daqu and the turbidity spoilage of traditional chinese vinegar.vinegar is a traditional fermented condiment, and the microbial diversity of daqu makes the quality of vinegar products. recently, turbidity spoilage of vinegar sharply tampered with the quality of vinegar. in this study, the relation between the microbial diversity of daqu and turbidity spoilage of vinegar was analyzed by plating technique, pcr-denaturing gradient gel electrophoresis (dgge), and high-performance liquid chromatography (hplc). the 16s rrna sequencing and dgge analysis indicated t ...201424691870
mixed food waste as renewable feedstock in succinic acid fermentation.mixed food waste, which was directly collected from restaurants without pretreatments, was used as a valuable feedstock in succinic acid (sa) fermentation in the present study. commercial enzymes and crude enzymes produced from aspergillus awamori and aspergillus oryzae were separately used in hydrolysis of food waste, and their resultant hydrolysates were evaluated. for hydrolysis using the fungal mixture comprising a. awamori and a. oryzae, a nutrient-complete food waste hydrolysate was genera ...201425149459
structural analysis of cerebrosides from aspergillus fungi: the existence of galactosylceramide in a. oryzae.glucosylceramide and galactosylceramide were detected in three aspergillus species: aspergillus oryzae, aspergillus sojae and aspergillus. awamori, using borate-coated tlc. the cerebrosides from a. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determined the composition of sugar, fatty acid and sphingoid base by gc/ms, maldi-tof/ms and (1)h-nmr. we identified them as β-glucosylceramide and β-galactosylceramide. the ceramide moiety ...201425129050
biodegradation of nicotine by a novel nicotine-degrading bacterium, pseudomonas plecoglossicida tnd35 and its new biotransformation intermediates.tobacco wastes that contain nicotine alkaloids are harmful to human health and the environment. in the investigation, a novel nicotine-biodegrading bacterium tnd35 was isolated and identified as pseudomonas plecoglossicida on the basis of phenotypic, biochemical characteristics and 16s rrna sequence homology. we have studied the nicotine biodegradation potential of strain tnd35 by detecting the intermediate metabolites using an array of approaches such as hplc, gc-ms, nmr and ft-ir. biotransform ...201423604517
conjugation of deoxynivalenol by alternaria alternata (54028 nrrl), rhizopus microsporus var. rhizopodiformis (54029 nrrl) and aspergillus oryzae (5509 nrrl).deoxynivalenol (don, vomitoxin) is a trichothecene mycotoxin which can be considered to be an indicator of fusarium mycotoxin contamination in grain, feed and food. recent studies have described the presence of glucose conjugated don, which is a product of plant metabolism, but there is a lack of information available on don conjugation by fungi. the aim of the current study was, therefore, to investigate the ability of fungi to metabolize don into hydrolysable conjugated don. alternaria alterna ...201424263850
fungal hydrolysis in submerged fermentation for food waste treatment and fermentation feedstock preparation.potential of fungal hydrolysis in submerged fermentation by aspergillus awamori and aspergillus oryzae as a food waste treatment process and for preparation of fermentation feedstock has been investigated. by fungal hydrolysis, 80-90% of the initial amount of waste was reduced and degraded within 36-48 h into glucose, free amino nitrogen (fan) and phosphate. experiments revealed that 80-90% of starch can be converted into glucose and highest concentration of fan obtained, when solid mashes of a. ...201424583214
the aspergillus genome database: multispecies curation and incorporation of rna-seq data to improve structural gene annotations.the aspergillus genome database (aspgd; is a freely available web-based resource that was designed for aspergillus researchers and is also a valuable source of information for the entire fungal research community. in addition to being a repository and central point of access to genome, transcriptome and polymorphism data, aspgd hosts a comprehensive comparative genomics toolbox that facilitates the exploration of precomputed orthologs among the 20 currently available asperg ...201424194595
upgrading the antioxidant potential of cereals by their fungal fermentation under solid-state cultivation conditions.solid-state fermentation (ssf) at 30°c for 72 h with four generally recognized as safe (gras) filamentous fungi (aspergillus oryzae ncim 1212, aspergillus awamori mtcc no. 548, rhizopus oligosporus ncim 1215 and rhizopus oryzae rck2012) showed high efficiency for the improvement of water-soluble total phenolic content (tpc) and antioxidant properties including abts(●+) [2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid)] and dpph(●) (2,2'-diphenyl-1-picrylhydrazyl) scavenging capacities of ...201424964826
drosera peltata smith var. lunata (buch.-ham.) c. b. clarke as a feasible source of plumbagin: phytochemical analysis and antifungal activity assay.drosera peltata smith var. lunata (buch.-ham.) c. b. clarke (dpvl) fractions and plumbagin were tested via broth microdilution techniques on rhizopus oryzae, aspergillus flavus, aspergillus niger, aspergillus oryzae, penicillium citrinum. all of the test substances [petroleum ether, chloroform, ethyl acetate, n-butanol fraction and aqueous residue (ar)] except for the ar were active against all the tested strains. the petroleum ether fraction (pef) was the most active (mic = 5.86-46.88 μg/ml, mf ...201424078108
non-aflatoxigenicity of commercial aspergillus oryzae strains due to genetic defects compared to aflatoxigenic aspergillus flavus.aspergillus oryzae is generally recognized as safe, but it is closely related to a. flavus in morphology and genetic characteristics. in this study, we tested the aflatoxigenicity and genetic analysis of nine commercial a. oryzae strains that were used in korean soybean fermented products. cultural and hplc analyses showed that none of the commercial strains produced detectable amount of aflatoxins. according to the molecular analysis of 17 genes in the aflatoxin (af) biosynthetic pathway, the c ...201424759426
fungal type iii polyketide synthases.this article covers the literature on fungal type iii polyketide synthases (pkss) published from 2005 to 2014. since the first discovery of fungal type iii pks genes in aspergillus oryzae, reported in 2005, putative genes for type iii pkss have been discovered in fungal genomes. compared with type i pkss, type iii pkss are much less abundant in fungi. however, type iii pkss could have some critical roles in fungi. this article summarizes the studies on fungal type iii pks functional analysis, in ...201425182423
strain development and optimized fermentation conditions for blood meal using aspergillus niger and aspergillus hydrolyze blood meal (bm) effectively, two mutants were generated using ultra-violet mutagenesis. single-factor experiments, the plackett-burman technique and response surface methodology were used to optimize the fermentation conditions. this study successfully generated a mutant and innovatively provided important parameters for utilizing bm by fermentation, which could be of industrial value.201424726870
antioxidant and antifungal activity of extracts of the aerial parts of thymus capitatus (l.) hoffmanns against four phytopathogenic fungi of citrus sinensis.many medicinal plants from the lamiaceae family can be easily found in algeria. these plants have been used as traditional medicines by local ethnic groups. thymus capitatus is known in algeria as "zaatar" and has been commonly used as a spice, and reported to have many biological effects.201424644439
conserved and varied dinucleotide sequences in the genomes of three aspergillus species.both guanine-cytosine content and nucleosome occupancy are higher in exons than in introns. in this study, the association between the frequencies of the dinucleotide sequences and the nucleosome occupancy of the exons and introns of the genes of aspergillus fumigatus, aspergillus nidulans, and aspergillus oryzae was studied. the frequency of the dinucleotide sequences aa (tt), at, and ta in the introns was more than that in the exons. in addition, the frequency of these sequences in the regions ...201425564023
fungal spore concentrations in indoor and outdoor air in university libraries, and their variations in response to changes in meteorological variables.the fungal spore concentration (fsc) in the air poses a risk for human health. this work studied the fsc in university libraries and how it is affected by environmental factors. a total of 347 samples were obtained using a microbio mb2(®) aerosol sampler. the wind speed (ws), cross wind (cw), temperature (t), relative humidity (hr), barometric pressure (bp) and dew point (dp) were recorded using a kestrel(®) 4500 weather station. the median indoor/outdoor fsc was 360/1230 cfu m(-3). fsc correlat ...201424070332
high sequence variations in the region containing genes encoding a cellular morphogenesis protein and the repressor of sexual development help to reveal origins of aspergillus oryzae.aspergillus oryzae and aspergillus flavus are closely related fungal species. the a. flavus morphotype that produces numerous small sclerotia (s strain) and aflatoxin has a unique 1.5 kb deletion in the norb-cypa region of the aflatoxin gene cluster (i.e. the s genotype). phylogenetic studies have indicated that an isolate of the nonaflatoxigenic a. flavus with the s genotype is the ancestor of a. oryzae. genome sequence comparison between a. flavus nrrl3357, which produces large sclerotia (l st ...201525689355
mechanistic aspects of biologically synthesized silver nanoparticles against food- and water-borne the present study, silver nanoparticles (agnps) synthesized from aqueous leaves extract of malva crispa and their mode of interaction with food- and water-borne microbes were investigated. formation of agnps was conformed through uv-vis, fe-sem, eds, afm, and hr-tem analyses. further the concentration of silver (ag) in the reaction mixture was conformed through icp-ms analysis. different concentration of nanoparticles (1-3 mm) tested to know the inhibitory effect of bacterial pathogens such a ...201526178241
the cell factory aspergillus enters the big data era: opportunities and challenges for optimising product with limits. getting more from less. producing commodities and high-value products from renewable resources including waste. what is the driving force and quintessence of bioeconomy outlines the lifestyle and product portfolio of aspergillus, a saprophytic genus, to which some of the top-performing microbial cell factories belong: aspergillus niger, aspergillus oryzae and aspergillus terreus. what makes them so interesting for exploitation in biotechnology and how can they help us to addr ...201525616499
genome mining for sesterterpenes using bifunctional terpene synthases reveals a unified intermediate of di/sesterterpenes.genome mining is a promising method to discover novel secondary metabolites in the postgenomic era. we applied the aspergillus oryzae heterologous expression system to functionally characterize cryptic bifunctional terpene synthase genes found in fungal genomes and identified the sesterfisherol synthase gene (nfss) from neosartorya fischeri. sesterfisherol contains a characteristic 5-6-8-5 tetracyclic ring system and is modified by cytochrome p450 monooxygenase (nfp450) to sesterfisheric acid. t ...201526332841
the mycobiota of air inside and outside the meju fermentation room and the origin of meju fungi.the fungi on meju are known to play an important role as degrader of macromolecule of soybeans. in order to elucidate the origin of fungi on traditional meju, mycobiota of the air both inside and outside traditional meju fermentation rooms was examined. from 11 samples of air collected from inside and outside of 7 meju fermentation rooms, 37 genera and 90 species of fungi were identified. in outside air of the fermentation room, cladosporium sp. and cladosporium cladosporioides were the dominant ...201526539041
production and characterization of two major aspergillus oryzae secreted prolyl endopeptidases able to efficiently digest proline-rich peptides of gliadin.prolyl endopeptidases are key enzymes in the digestion of proline-rich proteins. fungal extracts rich in prolyl endopeptidases produced by a species such as aspergillus oryzae used in food fermentation would be of particular interest for the development of an oral enzyme therapy product in patients affected by intolerance to gluten. two major a. oryzae secreted prolyl endopeptidases of the merops s28 peptidase family, aos28a and aos28b, were identified when this fungus was grown at acidic ph in ...201526464108
structural basis for the formation of acylalkylpyrones from two β-ketoacyl units by the fungal type iii polyketide synthase csyb.the acylalkylpyrone synthase csyb from aspergillus oryzae catalyzes the one-pot formation of the 3-acyl-4-hydroxy-6-alkyl-α-pyrone scaffold from acetoacetyl-coa, fatty acyl-coa, and malonyl-coa. this is the first type iii polyketide synthase that performs not only the polyketide chain elongation but also the condensation of two β-ketoacyl units. the crystal structures of wild-type csyb and its i375f and i375w mutants were solved at 1.7-, 2.3-, and 2.0-å resolutions, respectively. the crystal str ...201525564614
ruta montana l. leaf essential oil and extracts: characterization of bioactive compounds and suppression of crown gall disease.the aims of this study were to assess the antimicrobial efficacy of the leaf essential oil and the leaf extracts of r. montana against botrytis cinerea, fusarium oxysporum, verticillium dahliae, aspergillus oryzae and fusarium solani. the oil (1.000 µg/disk) and the extracts (1.500 µg/disk) revealed a remarkable antifungal effect against the tested plant pathogenic fungi with a radial growth inhibition percentage of 40.0-80.0 % and 5.0-58.0 %, respectively along with their respective mic values ...201526417353
nicotine-degrading microorganisms and their potential applications.nicotine-degrading microorganisms (ndms) are a special microbial group which can use nicotine as the sole carbon and nitrogen source for growth. since the 1950s, the bioconversion of nicotine by microbes has received increasing attention, and several ndms have been identified, such as arthrobacter nicotinovorans, microsporum gypseum, pellicularia filamentosa jts-208, and pseudomonas sp. 41. in recent years, increasing numbers of ndms have been isolated and identified from tobacco plantation soil ...201525805341
soil fungi for mycoremediation of arsenic pollution in agriculture soils.soil arsenic (as) contamination of food-chains and public health can be mitigated through fungal bioremediation. to enumerate culturable soil fungi, soils were collected from the as-contaminated paddy fields (3-35 mg kg(-1) ) of the middle indo-gangetic plains.201526348882
construction of a shuttle vector for heterologous expression of a novel fungal α-amylase gene in aspergillus oryzae.the filamentous fungus aspergillus oryzae is a well-known expression host used to express homologous and heterologous proteins in a number of industrial applications. to facilitate higher yields of proteins of interest, we constructed the pasop vector to express heterologous proteins in a. oryzae. pasop carries a selectable marker, pyrg, derived from aspergillus nidulans, and a strong promoter and a terminator of the amyb gene derived from a. oryzae. pasop transformed a. oryzae efficiently via t ...201525674804
increased production of free fatty acids in aspergillus oryzae by disruption of a predicted acyl-coa synthetase gene.fatty acids are attractive molecules as source materials for the production of biodiesel fuel. previously, we attained a 2.4-fold increase in fatty acid production by increasing the expression of fatty acid synthesis-related genes in aspergillus oryzae. in this study, we achieved an additional increase in the production of fatty acids by disrupting a predicted acyl-coa synthetase gene in a. oryzae. the a. oryzae genome is predicted to encode six acyl-coa synthetase genes and disruption of ao0900 ...201525564035
immobilization, regiospecificity characterization and application of aspergillus oryzae lipase in the enzymatic synthesis of the structured lipid 1,3-dioleoyl-2-palmitoylglycerol.the enzymatic synthesis of 1,3-dioleoyl-2-palmitoylglycerol (opo), one of the main components of human milk fats, has been hindered by the relatively high cost of sn-1,3-specific lipases and the deficiency in biocatalyst stability. the sn-1,3-specific lipase from aspergillus oryzae (aol) is highly and efficiently immobilized with the polystyrene-based hydrophobic resin d3520, with a significant 49.54-fold increase in specific lipase activity compared with the aol powder in catalyzing the synthes ...201526218640
entry inhibition of influenza viruses with high mannose binding lectin esa-2 from the red alga eucheuma serra through the recognition of viral hemagglutinin.lectin sensitivity of the recent pandemic influenza a virus (h1n1-2009) was screened for 12 lectins with various carbohydrate specificity by a neutral red dye uptake assay with mdck cells. among them, a high mannose (hm)-binding anti-hiv lectin, esa-2 from the red alga eucheuma serra, showed the highest inhibition against infection with an ec50 of 12.4 nm. moreover, esa-2 exhibited a wide range of antiviral spectrum against various influenza strains with ec50s of pico molar to low nanomolar leve ...201526035023
dietary supplementation with bacillus subtilis, saccharomyces cerevisiae and aspergillus oryzae enhance immunity and disease resistance against aeromonas hydrophila and streptococcus iniae infection in juvenile tilapia oreochromis niloticus.a feeding trial was conducted to investigate the effects of dietary administration of probiotic with bacillus subtilis, aspergillus oryzae and saccharomyces cerevisiae on growth, innate immune response, hemato-immunological parameters and disease resistance of nile tilapia, oreochromis niloticus. animals were distributed in three equal groups, each of five replicates and received one of the following experimental diets for four weeks: control, non-supplemented diet; 5 g kg(-1) probiotic mixture ...201525530581
valorisation of mixed bakery waste in non-sterilized fermentation for l-lactic acid production by an evolved thermoanaerobacterium sp. this study, an advanced biorefinery technology that uses mixed bakery waste has been developed to produce l-lactic acid using an adaptively evolved thermoanaerobacterium aotearoense la1002-g40 in a non-sterilized system. under these conditions, mixed bakery waste was directly hydrolysed by aspergillus awamori and aspergillus oryzae, resulting in a nutrient-rich hydrolysate containing 83.6g/l glucose, 9.5 g/l fructose and 612 mg/l free amino nitrogen. t. aotearoense la1002-g40 was evaluated an ...201526363501
batch dark fermentation from enzymatic hydrolyzed food waste for hydrogen production.a combination bioprocess of solid-state fermentation (ssf) and dark fermentative hydrogen production from food waste was developed. aspergillus awamori and aspergillus oryzae were utilized in ssf from food waste to generate glucoamylase and protease which were used to hydrolyze the food waste suspension to get the nutrients-rich (glucose and free amino nitrogen (fan)) hydrolysate. both glucose and fan increased with increasing of food waste mass ratio from 4% to 10% (w/v) and the highest glucose ...201525978853
[isolation and study on the aflatoxin genes of aflatoxin-producing fungi in paprika samples in chengdu].to isolate aflatoxin-producing strains from paprika samples and to do a preliminarily study on the relationship between aflatoxin-producing ability and the genes aflr, omt-1 and ver-1.201526619535
distinguishing the dominant species of pathogen in maxillary sinusitis by sequencing dna dataset analysis.this study determined the microbial composition in maxillary sinusitis and determined the predominant fungal and bacterial species. samples were obtained from 11 patients with fungal sinusitis and 2 healthy patients. the 13 samples were sequenced using an illumina hi-seq 2000, and mapping to the human, bacterial, and the fungal genomes were based on several steps. bioinformatics and statistical analyses were then performed for bacterial and fungal expressions in the samples. all samples were cul ...201525724392
cyanidin and malvidin in aqueous extracts of black carrots fermented with aspergillus oryzae prevent the impairment of energy, lipid and glucose metabolism in estrogen-deficient rats by ampk carrots (daucus carota l.) are rich in anthocyanins which contribute many health benefits, but are limited by bioavailability and instability when exposed to oxygen, heat and light. fermenting black carrots may improve the stability, absorption and bioactivity of its anthocyanins. here, we examined whether and by what mechanisms the long-term consumption of unfermented black carrot extract (bc) and its extracts fermented with lactobacillus plantarum (bclp) or aspergillus oryzae (bcao) migh ...201525701199
insecticidal activity of ethyl acetate extracts from culture filtrates of mangrove fungal the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude ethyl acetate culture filtrate extracts from 110 mangrove fungal endophytes were screened for their toxicity. toxicity tests of all extracts against brine shrimp (artemia salina) larvae were performed. the extracts with the highest toxicity were further examined for insecticidal activity against spodoptera litura larvae and acetylcholinesterase (ache) inhibition activity. the results showed th ...201526190921
ethanol and protein from ethanol plant by-products using edible fungi neurospora intermedia and aspergillus oryzae.feasible biorefineries for production of second-generation ethanol are difficult to establish due to the process complexity. an alternative is to partially include the process in the first-generation plants. whole stillage, a by-product from dry-mill ethanol processes from grains, is mostly composed of undegraded bran and lignocelluloses can be used as a potential substrate for production of ethanol and feed proteins. ethanol production and the proteins from the stillage were investigated using ...201526682213
antioxidant activity and anti-inflammatory activity of ethanol extract and fractions of doenjang in lps-stimulated raw 264.7 macrophages.fermentation can increase functional compounds in fermented soybean products, thereby improving antioxidant and/or anti-inflammatory activities. we investigated the changes in the contents of phenolics and isoflavones, antioxidant activity and anti-inflammatory activity of doenjang during fermentation and aging.201526634044
effect of canavalia gladiata extract fermented with aspergillus oryzae on the development of atopic dermatitis in nc/nga mice.canavalia gladiata has been used as a chinese traditional folk medicine for its anti-inflammatory properties. however, the use of c. gladiata is limited because it contains antinutritional and allergy-causing proteins. we fermented c. gladiata with aspergillus oryzae and investigated the effects of fermented c. gladiata (fcg) on the development of atopic dermatitis (ad) in mice. the mice were divided into five groups: untreated balb/c mice; ad control (nc/nga mice); fcgh (nc/nga mice fed a dieta ...201526613583
lignin degradation in corn stalk by combined method of h2o2 hydrolysis and aspergillus oryzae cgmcc5992 liquid-state fermentation.lignin peroxidase (lip) is the primary enzyme responsible for lignin degradation. in our previous work, in order to shorten the pretreatment time and increase the lignin degradation, we have pretreated the corn stalk (cs) using a combination of aspergillus oryzae cgmcc 5992 solid-state fermentation and h2o2 treatment.201526594239
preventive effects of fermented brown rice and rice bran against n-nitrosobis (2-oxopropyl) amine-induced pancreatic tumorigenesis in male hamsters.fermented brown rice by aspergillus oryzae (fbra) is known to have the potential to prevent chemical carcinogenesis of the colon, liver, esophagus, urinary bladder, stomach and lungs in rodents. the present study examined the possible chemopreventive effects of fbra on n-nitrosobis(2-oxopropyl)amine (bop)-induced pancreatic tumorigenesis in hamsters. five-week-old male syrian golden hamsters were divided into seven groups. groups 1-5 were subcutaneously injected with bop (10 mg/kg body weight) f ...201526788138
effect of different soybean meal type on ileal digestibility of amino acid in weaning experiment was conducted to evaluate apparent (aid) and standardized (sid) ileal digestibilities of crude protein (cp) and amino acids (aa) with 6 soybean products in weaning pigs. a total of 14 weaning barrows with an initial body weight of 6.54 ± 0.34 kg were fitted with t-cannula at the distal ileum and allotted to 7 diets containing various soybean products. the soybean products used in the experiment were conventional soybean meal (csbm), sbm fermented by aspergillus oryzae gb-107 (fsbma ...201526290731
inhibition of melanogenesis by yeast extracts from cultivations of recombinant pichia pastoris catalyzing ortho-hydroxylation of flavonoids.the inhibition of melanogenesis by yeast extracts from cultivations of recombinant pichia pastoris catalyzing ortho-hydroxylation of flavonoids was investigated. the recombinant yeast harbored a fusion gene composed of the cyp57b3 gene from aspergillus oryzae and a cytochrome reductase gene from saccharomyces cerevisiae. ten flavonoids belonging to flavones, flavonols, flavanones, flavanols, and isoflavones were evaluated for biotransformation by the recombinant strain. the results showed that f ...201526278529
soluble extract of soybean fermented with aspergillus oryzae gb107 inhibits fat accumulation in cultured 3t3-l1 adipocytes.this study was conducted to investigate the effects of fermented soybean (fs) extract on adipocyte differentiation and fat accumulation using cultured 3t3-l1 adipocytes.201526244085
cyclopiazonic acid biosynthesis gene cluster gene cpam is required for speradine a biosynthesis.speradine a is a derivative of cyclopiazonic acid (cpa) found in culture of an aspergillus tamarii isolate. heterologous expression of a predicted methyltransferase gene, cpam, in the cpa biosynthesis gene cluster of a. tamarii resulted in the speradine a production in a 2-oxocpa producing a. oryzae strain, indicating cpam is involved in the speradine a biosynthesis.201526207447
a new electrochemical substrate for rapid and sensitive in vivo monitoring of β-galactosidase gene expressions.a 4-methoxyphenyl-β-galactopyranoside (4-mpgal) substrate incorporating 4-methoxy phenol (4-mp) as an electrochemical reporter is described for the monitoring of β-galactosidase (β-gal) gene expressions. β-gal derived from escherichia coli (e. coli) and aspergillus oryzae (a. oryzae) were investigated, while a graphene oxide film modified electrode was employed as the transducer. the electrochemical signal of 4-mpg within 4-mpgal was masked by protecting their hydroxyl group with galactose. the ...201526192109
effect of novel starter culture on reduction of biogenic amines, quality improvement, and sensory properties of doenjang, a traditional korean soybean fermented sauce select appropriate microorganisms as starter cultures for the reliable and reproducible fermentation of soybean fermented products of korean doenjang, various ratios of fungi (aspergillus oryzae j, mucor racemosus 15, m. racemosus 42) combined with bacillus subtilis tksp 24 were selected as either single, double, or multiple meju strains for commercial mass production of doenjang, followed by analysis of sensory characteristics. in the sensory evaluation, doenjang bam15-1 and bam42-1, which w ...201526147854
synthesis and evaluation of galacto-noeurostegine and its 2-deoxy analogue as glycosidase epimer of the known glycosidase inhibitor noeurostegine, galacto-noeurostegine, was synthesised in 21 steps from levoglucosan and found to be a potent, competitive and highly selective galactosidase inhibitor of aspergillus oryzae β-galactosidase. galacto-noeurostegine was not found to be an inhibitor of green coffee bean α-galactosidase, yeast α-glucosidase and e. coli β-galactosidase, whereas potent but non-competitive inhibition against sweet almond β-glucosidase was established. the 2-deo ...201526111992
cooperative role of calnexin and tiga in aspergillus oryzae glycoprotein folding.calnexin (cnx), known as a lectin chaperone located in the endoplasmic reticulum (er), specifically recognizes g1m9gn2-proteins and facilitates their proper folding with the assistance of erp57 in mammalian cells. however, it has been left unidentified how cnx works in aspergillus oryzae, which is a filamentous fungus widely exploited in biotechnology. in this study, we found that a protein disulfide isomerase homolog tiga can bind with a. oryzae cnx (aocnx), which was revealed to specifically r ...201526085184
simultaneous synthesis of mixtures of lactulose and galacto-oligosaccharides and their selective fermentation.lactulose and galacto-oligosaccharides (gos) are well recognized prebiotics derived from lactose. in the synthesis of lactulose with β-galactosidases gos are also produced, but the ratio of lactulose and gos in the product can be tuned at will, depending on the operation conditions, so to obtain an optimal product distribution in terms of prebiotic potential. the selectivity of fermentation of each carbohydrate alone as well as mixtures of both was determined using ph-controlled anaerobic batch ...201526080080
fermented brown rice and rice bran with aspergillus oryzae (fbra) prevents inflammation-related carcinogenesis in mice, through inhibition of inflammatory cell infiltration.we have established an inflammation-related carcinogenesis model in mouse, in which regressive qr-32 cells subcutaneously co-implanted with a foreign body-gelatin sponge-convert themselves into lethal tumors due to massive infiltration of inflammatory cells into the sponge. animals were fed with a diet containing 5% or 10% fermented brown rice and rice bran with aspergillus oryzae (fbra). in 5% and 10% fbra diet groups, tumor incidences were lower (35% and 20%, respectively) than in the non-trea ...201526670250
towards effective and stable probiotics.probiotics are live microorganisms, generally either lactobacilli or bifidobacteria, which when administered in adequate amounts confer a health benefit to the host [1]. due to the growing evidence of health benefits associated with their use, probiotics are of increasing interest and represent now a significant growth area in the functional foods industry [2]. however, to be effective, orally administered probiotics should survive preparation of dosage forms and passage through acidic environme ...201526639716
lactose hydrolysis in milk and dairy whey using microbial β-galactosidases.this work aimed at evaluating the influence of enzyme concentration, temperature, and reaction time in the lactose hydrolysis process in milk, cheese whey, and whey permeate, using two commercial β-galactosidases of microbial origins. we used aspergillus oryzae (at temperatures of 10 and 55°c) and kluyveromyces lactis (at temperatures of 10 and 37°c) β-galactosidases, both in 3, 6, and 9 u/ml concentrations. in the temperature of 10°c, the k. lactis β-galactosidase enzyme is more efficient in th ...201526587283
deciphering the signaling mechanisms of the plant cell wall degradation machinery in aspergillus oryzae.the gene expression and secretion of fungal lignocellulolytic enzymes are tightly controlled at the transcription level using independent mechanisms to respond to distinct inducers from plant biomass. an advanced systems-level understanding of transcriptional regulatory networks is required to rationally engineer filamentous fungi for more efficient bioconversion of different types of biomass.201526573537
[novel type iii polyketide synthase csyb from aspergillus oryzae]. 201526571621
safety evaluation of amp deaminase from aspergillus oryzae.adenosine-5'-monophosphate (amp) deaminase is an enzyme used to increase concentrations of 5'-inosine monophosphate in certain foods and beverages for flavoring purposes. one commercial source of this enzyme is aspergillus oryzae, a filamentous fungus with a history of safe use in asia as a fermentation organism used in the production of miso sauce and sake liquors. noting the use of the enzyme in food intended for human consumption and potential presence at trace levels in finished goods, a ser ...201526559900
unfolded protein response is required for aspergillus oryzae growth under conditions inducing secretory hydrolytic enzyme production.unfolded protein response (upr) is an intracellular signaling pathway for adaptation to endoplasmic reticulum (er) stress. in yeast upr, ire1 cleaves the unconventional intron of hac1 mrna, and the functional hac1 protein translated from the spliced hac1 mrna induces the expression of er chaperone genes and er-associated degradation genes for the refolding or degradation of unfolded proteins. in this study, we constructed an irea (ire1 ortholog) conditionally expressing strain of aspergillus ory ...201526496881
molecular characterization of a penicillium chrysogenum exo-rhamnogalacturonan lyase that is structurally distinct from other polysaccharide lyase family proteins.we previously described an endo-acting rhamnogalacturonan (rg) lyase, termed pcrgl4a, of penicillium chrysogenum 31b. here, we describe a second rg lyase, called pcrglx. we determined the cdna sequence of the pcrglx gene, which encodes pcrglx. based on analyses using a blast search and a conserved domain search, pcrglx was found to be structurally distinct from known rg lyases and might belong to a new polysaccharide lyase family together with uncharacterized fungal proteins of nectria haematoco ...201525921806
hybrid de novo genome assembly using miseq and solid short read data.a hybrid de novo assembly pipeline was constructed to utilize both miseq and solid short read data in combination in the assembly. the short read data were converted to a standard format of the pipeline, and were supplied to the pipeline components such as abyss and soapdenovo. the assembly pipeline proceeded through several stages, and either miseq paired-end data, solid mate-paired data, or both of them could be specified as input data at each stage separately. the pipeline was examined on the ...201525919614
signal peptide optimization tool for the secretion of recombinant protein from saccharomyces cerevisiae.the secretion efficiency of foreign proteins in recombinant microbes is strongly dependent on the combination of the signal peptides (sps) used and the target proteins; therefore, identifying the optimal sp sequence for each target protein is a crucial step in maximizing the efficiency of protein secretion in both prokaryotes and eukaryotes. in this study, we developed a novel method, named the sp optimization tool (spot), for the generation and rapid screening of a library of sp-target gene fus ...201525912446
galacto configured n-aminoaziridines: a new type of irreversible inhibitor of β-galactosidases.a new type of galactose mimetics has been synthesized following a straightforward synthetic approach based on cyclohexene olefin aziridination reactions directed by hydroxyl substituents. these enantiomerically pure galacto-configured n-aminoaziridines are potent irreversible inhibitors of aspergillus oryzae and escherichia coliβ-galactosidases.201525895752
survey of protein-dna interactions in aspergillus oryzae on a genomic scale.the genome-scale delineation of in vivo protein-dna interactions is key to understanding genome function. only ∼5% of transcription factors (tfs) in the aspergillus genus have been identified using traditional methods. although the aspergillus oryzae genome contains >600 tfs, knowledge of the in vivo genome-wide tf-binding sites (tfbss) in aspergilli remains limited because of the lack of high-quality antibodies. we investigated the landscape of in vivo protein-dna interactions across the a. ory ...201525883143
fungal hemicellulose-degrading enzymes cause physical property changes concomitant with solubilization of cell wall polysaccharides.physical properties of wheat coleoptile segments decreased after treatment with hemicellulose-degrading enzymes, indicating that hemicellulosic polysaccharides function to control the strength of primary cell walls. changes in the physical properties of plant cell walls, a viscoelastic structure, are thought to be one of the growth-limiting factors for plants and one of the infection-affecting factors for fungi. to study the significance of hemicellulosic polysaccharides that form cross-bridges ...201525301670
telomere-mediated chromosomal truncation in aspergillus oryzae.we truncated the short arm of chromosome 3 to delete the aflatoxin biosynthesis gene homolog cluster using telomeric repeats in aspergillus oryzae. the predicted deletion was confirmed by southern blot analyses. this telomere-mediated chromosomal truncation method enables the development of an artificial chromosome in a. oryzae.201525034635
modulating endoplasmic reticulum-golgi cargo receptors for improving secretion of carrier-fused heterologous proteins in the filamentous fungus aspergillus oryzae.filamentous fungi are excellent hosts for industrial protein production due to their superior secretory capacity; however, the yield of heterologous eukaryotic proteins is generally lower than that of fungal or endogenous proteins. although activating protein folding machinery in the endoplasmic reticulum (er) improves the yield, the importance of intracellular transport machinery for heterologous protein secretion is poorly understood. here, using aspergillus oryzae as a model filamentous fungu ...201525362068
a combination of acid lactase from aspergillus oryzae and yogurt bacteria improves lactose digestion in lactose maldigesters synergistically: a randomized, controlled, double-blind cross-over trial.lactose digestion can be improved in subjects with impaired or completely absent intestinal lactase activity by administration of lactase preparations and particularly of acid lactase, which is active in the stomach, or by yogurt containing live lactic acid bacteria. it is the question, if lactose digestion can be further enhanced by combining these two approaches.201525042846
regulating yeast flavor metabolism by controlling saccharification reaction rate in simultaneous saccharification and fermentation of chinese maotai-flavor liquor.maotai-flavor liquor is produced by simultaneous saccharification and fermentation (ssf), in which filamentous fungi produce hydrolases to degrade the starch into fermentable sugar. saccharomyces cerevisiae simultaneously transforms the sugars to ethanol and flavor compounds. the saccharification rate plays an important role in regulating the liquor yield and flavor profile. this work investigated the effect of saccharification rate on fermentation by regulating the inoculation ratio (1:0.1, 1:0 ...201525676241
monitoring fungal growth on brown rice grains using rapid and non-destructive hyperspectral imaging.this research aimed to develop a rapid, non-destructive, and accurate method based on hyperspectral imaging (hsi) for monitoring spoilage fungal growth on stored brown rice. brown rice was inoculated with a non-pathogenic strain of aspergillus oryzae and stored at 30 °c and 85% rh. growth of a. oryzae on rice was monitored using viable colony counts, expressed as colony forming units per gram (cfu/g). the fungal development was observed using scanning electron microscopy. the hsi system was used ...201525662486
deep rna sequencing reveals a high frequency of alternative splicing events in the fungus trichoderma longibrachiatum.alternative splicing is crucial for proteome diversity and functional complexity in higher organisms. however, the alternative splicing landscape in fungi is still elusive.201525652134
computational study of β-n-acetylhexosaminidase from talaromyces flavus, a glycosidase with high substrate flexibility.β-n-acetylhexosaminidase (gh20) from the filamentous fungus talaromyces flavus, previously identified as a prominent enzyme in the biosynthesis of modified glycosides, lacks a high resolution three-dimensional structure so far. despite of high sequence identity to previously reported aspergillus oryzae and penicilluim oxalicum β-n-acetylhexosaminidases, this enzyme tolerates significantly better substrate modification. understanding of key structural features, prediction of effective mutants and ...201525627923
aspergillus oryzae s2 alpha-amylase production under solid state fermentation: optimization of culture conditions.aspergillus oryzae s2 was assayed for alpha-amylase production under solid state fermentation (ssf). in addition to amya and amyb already produced in monitored submerged culture, the strain was noted to produce new amyb oligomeric forms, in particular a dominant tetrameric form named amyc. the latter was purified to homogeneity through fractional acetone precipitation and size exclusion chromatography. sds-page and native page analyses revealed that, purified amyc was an approximately 172 kda te ...201525617840
purification and characterization of a thermostable hypothetical xylanase from aspergillus oryzae the current study, fermentation broth of aspergillus oryzae hml366 in sugar cane bagasse was subjected to ultrafiltration and ion exchange chromatography, and two xylanases, xynh1 and xynh2, were purified. time-of-flight mass spectrometry coupled with sds-page analysis revealed that xynh1 is identical to the hypothetical a. oryzae rib40 protein xp_001826985.1, with a molecular weight of 33.671 kda. likewise, xynh2 was identified as xylanase xynf1 with a molecular weight of 35.402 kda. sequenc ...201525604952
distinct mechanism of activation of two transcription factors, amyr and malr, involved in amylolytic enzyme production in aspergillus oryzae.the production of amylolytic enzymes in aspergillus oryzae is induced in the presence of starch or maltose, and two zn2cys6-type transcription factors, amyr and malr, are involved in this regulation. amyr directly regulates the expression of amylase genes, and malr controls the expression of maltose-utilizing (mal) cluster genes. deletion of malr gene resulted in poor growth on starch medium and reduction in α-amylase production level. to elucidate the activation mechanisms of these two transcri ...201525487891
the ctng gene encodes carbonic anhydrase involved in mycotoxin citrinin biosynthesis from monascus aurantiacus.citrinin, a fungal secondary metabolite of polyketide origin, is moderately nephrotoxic to vertebrates, including humans. citrinin is synthesised by condensation of acetyl-coa and malonyl-coa. six genes involved in the citrinin biosynthesis, including pksct, ctna and ctnb, have been cloned in monascus purpureus. the pksct gene encodes a polyketide synthase; ctna is a regulatory factor; and ctnb encodes an oxidoreductase. when the three genes were respectively disrupted, the disruption strains dr ...201525482072
cloning and expression of a novel prolyl endopeptidase from aspergillus oryzae and its application in beer stabilization.a novel prolyl endopeptidase gene from aspergillus oryzae was cloned and expressed in pichia pastoris. amino acid sequence analysis of the prolyl endopeptidase from aspergillus oryzae (ao-pep) showed that this enzyme belongs to a class serine peptide s28 family. expression, purification and characterization of ao-pep were analyzed. the optimum ph and temperature were ph 5.0 and 40 °c, respectively. the enzyme was activated and stabilized by metal ion ca(2+) and inhibited by zn(2+), mn(2+), al(3+ ...201525547787
flavourzyme, an enzyme preparation with industrial relevance: automated nine-step purification and partial characterization of eight enzymes.flavourzyme is sold as a peptidase preparation from aspergillus oryzae. the enzyme preparation is widely and diversely used for protein hydrolysis in industrial and research applications. however, detailed information about the composition of this mixture is still missing due to the complexity. the present study identified eight key enzymes by mass spectrometry and partially by activity staining on native polyacrylamide gels or gel zymography. the eight enzymes identified were two aminopeptidase ...201525996918
ionic interaction of positive amino acid residues of fungal hydrophobin rola with acidic amino acid residues of cutinase cutl1.hydrophobins are amphipathic proteins secreted by filamentous fungi. when the industrial fungus aspergillus oryzae is grown in a liquid medium containing the polyester polybutylene succinate co-adipate (pbsa), it produces rola, a hydrophobin, and cutl1, a pbsa-degrading cutinase. secreted rola attaches to the surface of the pbsa particles and recruits cutl1, which then condenses on the particles and stimulates the hydrolysis of pbsa. here, we identified amino acid residues that are required for ...201525588312
change in enzyme production by gradually drying culture substrate during solid-state fermentation.the influence of drying the culture substrate during solid-state fermentation on enzyme production was investigated using a non-airflow box. the drying caused a significant increase in enzyme production, while the mycelium content decreased slightly. this suggests that changes in the water content in the substrate during culture affect enzyme production in fungi.201525488496
biochemical evaluation of xylanases from various filamentous fungi and their application for the deinking of ozone treated newspaper pulp.filamentous fungi, aspergillus oryzae mdu-4 was biochemically selected among different species of aspergillus and trichoderma, for xylanase production. the enzyme activity and specific activity of partially purified xylanase from a. oryzae mdu-4 was 7452 iu/ml and 13,549 iu/g, respectively. temperature and ph optima for xylanase were found to be 60°c and 6.0, respectively. the reaction kinetics of xylanase was found to be km (3.33 mg/ml) and vmax (18,182 μmol/mg). the implementation of ozone tre ...201525965456
effects of dietary cellulase and xylanase addition on digestion, rumen fermentation and methane emission in growing goats.the objective of this study was to evaluate the effectiveness of supplementation of cellulase and xylanase to diets of growing goats to improve nutrient digestibility, utilisation of energy and mitigation of enteric methane emissions. the experiment was conducted in a 5 × 5 latin square design using five goats with permanent rumen fistulae and five treatments consisted of two levels of cellulase crossed over with two levels of xylanase plus unsupplemented control. the cellulase (243 u/g) derived ...201525963843
effect of the dose of exogenous fibrolytic enzyme preparations on preingestive fiber hydrolysis, ruminal fermentation, and in vitro digestibility of bermudagrass haylage.our objectives were to evaluate the effects of the dose rates of 5 trichoderma reesei and aspergillus oryzae exogenous fibrolytic enzymes (efe; 1a, 2a, 11c, 13d, and 15d) on in vitro digestibility, fermentation characteristics, and preingestive hydrolysis of bermudagrass haylage and to identify the optimal dose of each efe for subsequent in vitro and in vivo studies. in experiment 1, efe were diluted in citrate-phosphate buffer (ph 6) and applied in quadruplicate in each of 2 runs at 0× (control ...201525468699
molecular characterization of intergeneric hybrid between aspergillus oryzae and trichoderma harzianum by protoplast fusion.protoplast fusion between aspergillus oryzae and trichoderma harzianum and application of fusant in degradation of shellfish waste.201525444252
purification and characterisation of α-amylase produced by mutant strain of aspergillus oryzae ems-18.α-amylase produced by a mutant strain of aspergillus oryzae ems-18 has been purified to homogeneity as judged by sodium dodecyle sulphate polyacrylamide gel electrophoresis (sds-page). the enzyme was purified by using 70% ammonium sulphate precipitation followed by anion exchange chromatography on deae-sephadex column and gel filtration on sephadex g-100. an enzyme purification factor of 9.5-fold was achieved with a final specific activity of 1987.7 u/mg protein and overall yield of 23.8%. the m ...201525424747
a multi-criteria analysis approach for ranking and selection of microorganisms for the production of oils for biodiesel production.oleaginous microorganisms have potential to be used to produce oils as alternative feedstock for biodiesel production. microalgae (chlorella protothecoides and chlorella zofingiensis), yeasts (cryptococcus albidus and rhodotorula mucilaginosa), and fungi (aspergillus oryzae and mucor plumbeus) were investigated for their ability to produce oil from glucose, xylose and glycerol. multi-criteria analysis (mca) using analytic hierarchy process (ahp) and preference ranking organization method for the ...201525958151
transcriptome and proteome expression analysis of the metabolism of amino acids by the fungus aspergillus oryzae in fermented soy sauce.amino acids comprise the majority of the flavor compounds in soy sauce. a portion of these amino acids are formed from the biosynthesis and metabolism of the fungus aspergillus oryzae; however, the metabolic pathways leading to the formation of these amino acids in a. oryzae remain largely unknown. we sequenced the transcriptomes of a. oryzae 100-8 and a. oryzae 3.042 under similar soy sauce fermentation conditions. 2d gel electrophoresis was also used to find some differences in protein express ...201525945335
isolation and expression of enolase gene in fusarium oxysporum f. sp. lycopersici.fusarium oxysporum f. sp. lycopersici is a fungus responsible for the tomato disease known as fusariosis. enolase, which is the enzyme that catalyzes the reaction of 2-phosphoglycerate to phosphoenolpyruvate, is present during glycolysis. enolase genes have been isolated from bacteria and fungi, among other organisms. in this research, a large portion of the enolase, eno, gene sequence was isolated from f. oxysporum and compared with those of other microorganisms, revealing a similarity of 51-69 ...201525349089
characterization of the production of biogenic amines and gamma-aminobutyric acid in the soybean pastes fermented by aspergillus oryzae and lactobacillus brevis.the production of gamma-aminobutyric acid (gaba) using gaba-producing lactic acid bacteria (lab) has been considered to be an attractive strategy. however, some lab may produce biogenic amines (ba), which may be of concern from the safety viewpoint. the aim of the present study was to characterize the production of gaba and ba in the soybean pastes fermented by aspergillus oryzae (a. oryzae) fmb s46471 and gaba-producing lactobacillus brevis (l. brevis) gaba 100. after a ripening period of 90 da ...201525341471
improvement of omega-3 docosahexaenoic acid production by marine dinoflagellate crypthecodinium cohnii using rapeseed meal hydrolysate and waste molasses as feedstock.rapeseed meal and waste molasses are two important agro-industrial by-products which are produced in large quantities. in this study, solid state fermentation and fungal autolysis were performed to produce rapeseed meal hydrolysate (rmh) using fungal strains of aspergillus oryzae, penicillium oxalicum and neurospora crassa. the hydrolysate was used as fermentation feedstock for heterotrophic growth of microalga crypthecodinium cohnii that produce docosahexaenoic acid (dha). the addition of waste ...201525942565
purification and enzymatic characterization of secretory glycoside hydrolase family 3 (gh3) aryl β-glucosidases screened from aspergillus oryzae a global search of the genome database of aspergillus oryzae, we found 23 genes encoding putative β-glucosidases, among which 10 genes with a signal peptide belonging to glycoside hydrolase family 3 (gh3) were overexpressed in a. oryzae using the improved glaa gene promoter. consequently, crude enzyme preparations from three strains, each harboring the genes ao090038000223 (bgla), ao090103000127 (bglf), and ao090003001511 (bglj), showed a substrate preference toward p-nitrophenyl-β-d-glucopyr ...201525936960
repeated-batch operation for the synthesis of lactulose with β-galactosidase immobilized by aggregation and crosslinking.synthesis of lactulose under repeated-batch operation was done with cross-linked aggregates of aspergillus oryzae β-galactosidase (clags). the effect of the crosslinking agent to enzyme mass ratio and cross-linking time were first evaluated. best results were obtained at 5.5gdeglutaraldehyde/g enzyme at 5h of cross-linking, obtaining a specific activity of 15,000iug(-1), with 30% immobilization yield. clag was more stable than the free enzyme under non-reactive conditions with a half-life of 123 ...201525935392
a fungal biofilm reactor based on metal structured packing improves the quality of a gla::gfp fusion protein produced by aspergillus oryzae.fungal biofilm is known to promote the excretion of secondary metabolites in accordance with solid-state-related physiological mechanisms. this work is based on the comparative analysis of classical submerged fermentation with a fungal biofilm reactor for the production of a gla::green fluorescent protein (gfp) fusion protein by aspergillus oryzae. the biofilm reactor comprises a metal structured packing allowing the attachment of the fungal biomass. since the production of the target protein is ...201525935344
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