| aspergillus fumigatus causes in vitro electrophysiological and morphological modifications in human nasal epithelial cells. | the role of the airway epithelium in the development of invasive aspergillosis in immunocompromised hosts has rarely been studied although patients at risk for this infection frequently have epithelial damage. we developed an in vitro model of primary culture of human nasal epithelial cells (hnec) in air-liquid interface, which allows epithelial cell differentiation and mimics in vivo airway epithelium. we subsequently tested 7-day and 24-hour aspergillus fumigatus filtrates on the apical side o ... | 2002 | 12371137 |
| tmc-256a1 and c1, new inhibitors of il-4 signal transduction produced by aspergillus niger var niger tc 1629. | new inhibitors of il-4 signal transduction, designated as tmc-256a1 and c1, were discovered together with tmc-256b1, a previously known dihydronaphthopyrone, from the fermentation broth of aspergillus niger var niger tc 1629 by using an il-4 driven reporter gene assay. based on spectroscopic analyses, tmc-256a1 and c1 were found to be new members of the naphthopyrone antibiotics. tmc-256a1, b1 and c1 inhibited the il-4 driven luciferase activity with ic50 values of 25 microm, 30 microm and 1.7 m ... | 2002 | 12374380 |
| enzymatic solubilization of arabinoxylans from isolated rye pentosans and rye flour by different endo-xylanases and other hydrolyzing enzymes. effect of a fungal caccase on the flour extracts oxidative gelation. | water-extractable (wep) and water-unextractable (wup) pentosans were isolated from a rye flour. the effect of a commercial enzyme preparation, grindamyl s 100 (gs100), containing pentosanase activities, was investigated on wep, wup, a mix of wep and wup, and the rye flour, with the aim to monitor the solubilization and depolymerization of high molecular weight arabinoxylans and the effect on the viscosity of the reaction medium. the effects of other hydrolyzing enzymes were also tested. three xy ... | 2002 | 12381136 |
| the beta-1,4-endogalactanase a gene from aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions. | the aspergillus nigerbeta-1,4-endogalactanase encoding gene (gala) was cloned and characterized. the expression of gala in a. niger was only detected in the presence of sugar beet pectin, d-galacturonic acid and l-arabinose, suggesting that gala is coregulated with both the pectinolytic genes as well as the arabinanolytic genes. the corresponding enzyme, endogalactanase a (gala), contains both active site residues identified previously for the pseudomonas fluorescensbeta-1,4-endogalactanase. the ... | 2002 | 12383257 |
| cloning and characterization of glucokinase from a methylotrophic yeast hansenula polymorpha: different effects on glucose repression in h. polymorpha and saccharomyces cerevisiae. | glucokinase gene (hpglk1) was cloned from a methylotrophic yeast hansenula polymorpha by complementation of glucose-phosphorylation deficiency in a h. polymorpha double kinase-negative mutant a31-10 by a genomic library. an open reading frame of 1416 nt encoding a 471-amino-acid protein with calculated molecular weight 51.6 kda was characterized in the genomic insert of the plasmid ph3. the protein sequence deduced from hpglk1 exhibited 55 and 46% identity with glucokinases from saccharomyces ce ... | 2002 | 12383517 |
| effects of the peptide mycotoxin destruxin e on insect haemocytes and on dynamics and efficiency of the multicellular immune reaction. | destruxins (dtxs) are cyclic peptide toxins secreted by the entomopathogenic fungus metarhizium anisopliae var. anisopliae. the effects of dtx e, the most active compound of this family on haemocytes, the immunocompetent insect cells, and on the dynamics and efficacy of the multicellular defense of insect hosts have been investigated. ultrastructural alterations have been observed in circulating plasmatocytes and granular haemocytes, and in attached haemocytes of galleria mellonella larvae treat ... | 2002 | 12384084 |
| the effects of temperature, salinity, and other factors on the growth and formation of uv-absorbing substances by the fungus aspergillus. | aspergillus niger can be grown at 263 degrees k in a kcl-saturated medium with glucose as the sole c-source. in spite of their similarities k and rb-ions are not equivalent, but unlike na-ion, both support growth. the dependency of growth rate upon the nitrogen source is peptone > nh4+ > no3-. in kcl-medium at 263 degrees k, the production of uv-absorbing aromatic substances normally elaborated by this fungus and soluble in alcohol is reduced more than 20-fold as indicated by comparisons of tota ... | 1978 | 12387289 |
| expression profiling of pectinolytic genes from aspergillus niger. | the expression of 26 pectinolytic genes from aspergillus niger was studied in a wild type strain and a crea derepressed strain, under 16 different growth conditions, to obtain an expression profile for each gene. these expression profiles were then submitted to cluster analysis to identify subsets of genes with similar expression profiles. with the exception of the feruloyl esterase encoding genes, all genes were expressed in the presence of d-galacturonic acid, polygalacturonate, and/or sugar b ... | 2002 | 12387863 |
| aspergillus niger endophthalmitis after cataract surgery. | we report a 65-year-old diabetic woman who developed aspergillus niger endophthalmitis after cataract surgery. she presented 9 weeks after extracapsular cataract extraction with a black growth covering the cornea and moderate echoes in the vitreous on ultrasonography. after microbiological confirmation of fungal endophthalmitis, the patient received intravitreal amphoterecin b 5 micro g, topical natamycin 5% hourly, atropine 1% 3 times, and oral antifungal therapy. the patient was told the visua ... | 2002 | 12388048 |
| preservative properties of calamintha officinalis essential oil with and without edta. | this study was focused on the preserving properties of calamintha officinalis essential oil, a plant known for its diaphoretic, expectorant and aromatic properties. | 2002 | 12390486 |
| spectrum of antimicrobial activity and user acceptability of the hand disinfectant agent sterillium gel. | the antimicrobial efficacy of alcohol-based hand gels has been shown to be significantly less than liquid hand rubs probably because of a lower concentration of alcohol. sterillium gel is the first hand gel with 85% ethanol. its antimicrobial efficacy and user acceptability was studied. bactericidal activity was tested according to pren 12054 against staphylococcus aureus, enterococcus hirae, pseudomonas aeruginosa and escherichia coli (suspension test) and en 1500 (15 volunteers; four replicate ... | 2002 | 12392906 |
| invertase production on solid-state fermentation by aspergillus niger strains improved by parasexual recombination. | invertase production by aspergillus niger grown by solid-state fermentation was found to be higher than by conventional submerged fermentation. the haploid mutant strains aw96-3 and aw96-4 showed better productivity of various enzymes, as compared to wild-type parental strain a. niger c28b25. here we use parasexual crosses of those mutants to increase further the productivity of invertase in solid-state fermentation. we isolated both a diploid (dar2) and an autodiploid (ad96-4) strain, which wer ... | 2002 | 12396111 |
| a biotechnological process involving filamentous fungi to produce natural crystalline vanillin from maize bran. | a new process involving the filamentous fungi aspergillus niger and pycnoporus cinnabarinus has been designed for the release of ferulic acid by enzymic degradation of a cheap and natural agricultural byproduct (autoclaved maize bran) and its biotransformation into vanillic acid and/or vanillin with a limited number of steps. on the one hand, the potentialities of a. niger i-1472 to produce high levels of polysaccharide-degrading enzymes including feruloyl esterases and to transform ferulic acid ... | 2002 | 12396118 |
| toxicity assessment of nickel using aspergillus niger and its removal from an industrial effluent. | chemical analysis of electroplating effluent revealed the presence of very high concentrations of nickel (393 ppm) in the effluent. bioassay was carried out to test the toxicity of nickel chloride to aspergillus niger. in contrast to 50% conidial inhibition at 1.7 mm nickel, hyphal extension was affected even at a lower concentration (0.4 mm), suggesting that hyphae are more sensitive than conidia to nickel. an increase in nickel concentration resulted in a proportionate decrease in the hyphal e ... | 2002 | 12396123 |
| potential use of cutinase in enzymatic scouring of cotton fiber cuticle. | the present study characterized the ability of a bacterial cutinase to improve the wettability of raw cotton fabrics by specific hydrolysis of the cutin structure of the cuticle. the effect of cutinase was studied alone and in coreaction with pectin lyase. the changes in both the fabric and the reaction fluid were measured and compared to enzymatic hydrolysis with polygalacturonase, and to chemical hydrolysis with boiling naoh. water absorbancy, specific staining, fabric weight loss, and evapora ... | 2002 | 12396130 |
| culture conditions dictate protease and tannase production in submerged and solid-state cultures of aspergillus niger aa-20. | undesirable protease production by aspergillus niger aa-20 in submerged culture and solid-state culture was evaluated using different concentrations of tannic acid as sole carbon source in a model system designed for tannase production. protease production was found to be dependent on the culture system used (submerged culture or solid-state culture) and on the initial tannic acid concentration. expression of protease activity in submerged culture was higher (up to 10 times) than activity obtain ... | 2002 | 12396141 |
| synthesis, structural characterization and antimicrobial studies of hydrazone derivatives of 3-hydroxyimino-5-methyl-2-hexanone. | the derivatives of 3-hydroxyimino-5-methyl-2-hexanone oxime have been obtained in good yield by its reactions either with hydrazine hydrate or phenyl hydrazine, respectively. ir and 1h nmr spectral data of these compounds have been discussed. all the newly synthesised compounds have been tested for their biological activity against s. auerus, s. typhi, c. albicans, a. niger, s. cerevisiae and m. tuberculosis h47rv. | 2002 | 12403304 |
| enzyme fingerprints by fluorogenic and chromogenic substrate arrays we would like to thank prof. r. furstoss and dr. a. archelas at the faculté des sciences de luminy, marseille, france, for providing a sample of aspergillus niger eh, and dr. c. weijers at the department of food technology and nutrition sciences, wageningen university, the netherlands, for providing a sample of rhodotorula glutinis eh. this work was supported by the university of bern, the european cost program (action d12), the swiss office fédéral de l'education et de la science, protéus sa (nîmes, france), the ministero della università e della ricerca scientifica e tecnologica (murst), and consiglio nazionale delle ricerche (cnr) (roma). p.c. gratefully acknowledges merck for generous financial support from a 2000 adp chemistry award. | | 2001 | 12404444 |
| a tanks-in-series bioreactor to simulate macromolecule-laden wastewater pretreatment under sewer conditions by aspergillus niger. | sewers are typically a means of transporting wastewater to a treatment facility, with little biotransformation of the soluble polymeric organic matter by suspended biomass. in the interest of providing an effective pretreatment of wastewater in a sewer network, it is necessary to design an accurate tool simulating sewer conditions and introduce an appropriate biomass for macromolecular pollutant degradation. such a model reactor was built using a tanks-in-series design and the degradation of a p ... | 2002 | 12405402 |
| comparative studies on extracellular protease secretion and glucoamylase production by free and immobilized aspergillus niger cultures. | the effects of cell immobilization on the secretion of extracellular proteases and glucoamylase production by aspergillus niger were investigated under a variety of immobilization techniques and culture conditions. immobilization was achieved by means of cell attachment on metal surfaces or spore entrapment and subsequent growth on porous celite beads. free-suspension cultures were compared with immobilized mycelium under culture conditions that included growth in shake flasks and an airlift bio ... | 2002 | 12407460 |
| regulation by carbon and nitrogen sources of a family of cellulases in aspergillus nidulans. | the total amount of aspergillus nidulans secreted cellulases is affected by both the carbon and nitrogen source present in the medium, and is regulated directly and/or indirectly by the carbon metabolism regulators, crea, creb, and crec, and the global nitrogen metabolism regulator, area. we have characterized two a. nidulans genes that encode exo-cellulases, and one gene that encodes an endo-cellulase which is additional to the previously described endo-cellulase encoding gene, egla. the putati ... | 2002 | 12409103 |
| detection of cell wall galactomannoprotein afmp1p in culture supernatants of aspergillus fumigatus and in sera of aspergillosis patients. | mannoproteins are important and abundant structural components of fungal cell walls. the afmp1 gene encodes a cell wall galactomannoprotein of aspergillus fumigatus. in the present study, we show that afmp1p is secreted into the cell culture supernatant at a level that can be detected by western blotting. a sensitive enzyme-linked immunosorbent assay (elisa) developed with antibodies against afmp1p was capable of detecting this protein from the cell culture supernatant of a. fumigatus. the anti- ... | 2002 | 12409437 |
| identification of amino acid residues critical for catalysis and stability in aspergillus niger family 1 pectin lyase a. | site-directed-mutagenesis studies were performed on family 1 pectin lyase a (pl1a) from aspergillus niger to gain insight into the reaction mechanism for the pectin lyase-catalysed beta-elimination cleavage of methylesterified polygalacturonic acid and to stabilize the enzyme at slightly basic ph. on the basis of the three-dimensional structures of pl1a [mayans, scott, connerton, gravesen, benen, visser, pickersgill and jenkins (1997) structure 5, 677-689] and the modelled enzyme-substrate compl ... | 2003 | 12418964 |
| environmental surveillance of filamentous fungi in three tertiary care hospitals in greece. | the environmental fungal load (fl) of three hospitals was studied in representative regions in greece (thessalonika, northern greece, athens, central greece and heraklion, southern greece). air, surfaces and tap water from high-risk departments were sampled monthly during one year. air fl was [median (range)] 10.6 (1.2-37), 5.5 (3-28.8) and 7.7 (3.1-12.1) cfu/m(3) at thessalonika, athens and heraklion, respectively. air fl was lower in winter and higher in summer and autumn but seldom above acce ... | 2002 | 12419271 |
| skin prick test reactivity to common aeroallergens in asthmatic patients with and without rhinitis. | the aim of this study was to investigate the prevalence of skin prick test (spt) reactivity to common aeroallergens among malaysian asthmatic patients with and without rhinitis. | 2002 | 12421243 |
| invasive aspergillosis in cancer. | ten confirmed cases of invasive aspergillosis (ia) in cancer patients were analysed retrospectively. eight were pulmonary, one was sinonasal and one was cutaneous. the majority of patients had haematological malignancies (7), the remaining three were cases of solid tumours. fever was present in all 10 cases. cough and lung signs were present in all eight cases of invasive pulmonary aspergillosis. haemoptysis was encountered in three of nine cases of pulmonary and sinonasal aspergillosis. mortali ... | 2002 | 12421281 |
| antimicrobial activity of berberine--a constituent of mahonia aquifolium. | the antimicrobial activity of the protoberberine alkaloid, berberine, isolated from mahonia aquifolium, was evaluated against 17 microorganisms including two gram-negative bacteria--pseudomonas aeruginosa and escherichia coli (both resistant and sensitive), two gram-positive bacteria--bacillus subtilis and staphylococcus aureus, zoogloea ramigera, six filamentous fungi--penicilium chrysogenum, aspergillus niger, aureobasidium pullulans (black and white strain), trichoderma viride (original green ... | 2002 | 12422513 |
| regulation of the production of polygalacturonase by aspergillus niger. | synthesis of ethylene in static cultures as well as the effect of endogenous and exogenous ethylene on the synthesis of polygalacturonase by aspergillus niger were determined. this strain produced maximum ethylene amounts when cultured at 30 degrees c for 3 d. the effect of adding ethylene precursors (citrate-cycle intermediates) on ethylene production was investigated. best intracellular and extracellular polygalacturonase production was obtained with 2-oxoglutaric, pyruvic and fumaric acids, a ... | 2002 | 12422519 |
| structure of the arabinogalactan from gum tragacanth (astralagus gummifer). | the polysaccharide obtained by ethanol precipitation from an aqueous solution of gum tragacanth contained arabinogalactan and tragacanthic acid, as well as starch ( approximately 0.6%). gc-ms, nmr, and esi-ms analyses showed the structure of the arabinogalactan to be even more complex than previously determined, with core structures containing arap, beta-araf, and alpha-galp units, as well as known terminal, and 2-o- and 3-o-substituted alpha-araf units. analysis was aided by examination of free ... | 2002 | 12423966 |
| a motif of a microbial starch-binding domain found in human genethonin. | the sequence of the starch-binding domain present in 10% of amylolytic enzymes of microbial origin and classified as the carbohydrate-binding module family 20, was identified in the equivalent part of sequence of human genethonin, a skeletal muscle protein of unknown function. the sequence identity between the starch-binding domain from bacillus sp. strain 1011 cyclodextrin glucanotransferase and the corresponding segment of human genethonin was higher than 28%. the amino acid residues known to ... | 2002 | 12424126 |
| differentiation of feruloyl esterases on synthetic substrates in alpha-arabinofuranosidase-coupled and ultraviolet-spectrophotometric assays. | 4-nitrophenyl 5-o-trans-feruloyl-alpha-l-arabinofuranoside and 4-nitrophenyl 2-o-trans-feruloyl-alpha-l-arabinofuranoside, synthesized by our group (m. mastihubová, j. szemesová, and p. biely), were found to be suitable substrates for determination of activity of feruloyl esterases (fees) exhibiting affinity for 5-o- and 2-o-feruloylated alpha-l-arabinofuranosyl residues. one assay is based on coupling the fee-catalyzed formation of 4-nitrophenyl alpha-l-arabinofuranoside with its efficient hydr ... | 2002 | 12441154 |
| morphological and enzymatic responses of a recombinant aspergillus niger to oxidative stressors in chemostat cultures. | continuous chemostat cultures of a recombinant strain of aspergillus niger (b1-d), engineered to produce the marker protein hen egg white lysozyme, were investigated with regard to their susceptibility to oxidative stress. the culture response to oxidative stress, produced either by addition of exogenous hydrogen peroxide (h(2)o(2)) or by high dissolved oxygen tension (dot), was characterised in terms of the activities of two key defensive enzymes: catalase (cat) and superoxide dismutase (sod). ... | 2003 | 12443856 |
| microbial metabolism of artemisinin by mucor polymorphosporus and aspergillus niger. | artemisinin (1) was transformed by mucor polymorphosporus and aspergillus niger. five products were identified as 9beta-hydroxyartemisinin (2), 3beta-hydroxyartemisinin (3), deoxyartemisinin (4), 3beta-hydroxydeoxyartemisinin (5), and 1alpha-hydroxydeoxyartemisinin (6). products 2, 3, and 6 are new compounds. | 2002 | 12444705 |
| physicochemical characterisation of the two active site mutants trp(52)-->phe and asp(55)-->val of glucoamylase from aspergillus niger. | glucoamylase 1 (ga1) from aspergillus niger is a multidomain starch hydrolysing enzyme that consists of a catalytic domain and a starch-binding domain connected by an o-glycosylated linker. the fungus also produces a truncated form without the starch-binding domain (ga2). the active site mutant trp(52)-->phe of both forms and the asp(55)-->val mutant of the ga1 form have been prepared and physicochemically characterised and compared to recombinant wild-type enzymes. the characterisation included ... | 2002 | 12445478 |
| synthesis and biological activity of isoamoenylin, a metabolite of dendrobium amoenum. | isoamoenylin (6), a dihydrostilbene from dendrobium amoenum, was synthesised from 3,4,5-trimethoxybenzaldehyde (1) in four steps with an overall yield of 60%. the spectral data for synthetic 6 are in good agreement with those of the natural product. isoamoenylin showed moderate antioxidative and weak antibacterial activities. | 2002 | 12450140 |
| do mitochondria regulate cellular iron homeostasis through citric acid and haem production? implications for cancer and other diseases. | citric acid is produced industrially by depriving aspergillus niger of iron. the lack of fe deactivates mitochondrial aconitase and interrupts the krebs cycle, causing the mitochondria to release citric acid as a siderophore (an fe getter). when the mitochondrion has plenty of fe and the cell has enough atp, aerobic phosphorylation stops and fatty acid or haem synthesis take place, when the cell has plenty of haem, haem synthesis stops. since most of the fe activity in the cell is related to the ... | 2003 | 12450775 |
| hydrolysis of lactose by free and immobilized beta-galactosidase from thermus sp. strain t2. | the hydrolysis of lactose by a beta-galactosidase from the thermophilic microorganism thermus sp. strain t2, both in solution and immobilized on a commercial silica-alumina, has been studied. the enzyme has been previously produced by escherichia coli jm101 harboring the plasmid pbgt1, which contains the codifying gene under the promoters lpp(p) and lac(pq). the enzyme was immobilized on the support activated with tris-hydroxymethylphosphine (thp). activity and stability of the free and the immo ... | 2003 | 12451560 |
| secreted proteases from pathogenic fungi. | many species of human pathogenic fungi secrete proteases in vitro or during the infection process. secreted endoproteases belong to the aspartic proteases of the pepsin family, serine proteases of the subtilisin family, and metalloproteases of two different families. to these proteases has to be added the non-pepsin-type aspartic protease from aspergillus niger and a unique chymotrypsin-like protease from coccidioides immitis. pathogenic fungi also secrete aminopeptidases, carboxypeptidases and ... | 2002 | 12452286 |
| detection of ochratoxin a in animal feeds and capacity to produce this mycotoxin by aspergillus section nigri in argentina. | ochratoxin a (oa) is a mycotoxin detected in a variety of food and feeds mostly from countries with a temperate climate because of the fungi that produce it, mainly aspergillus ochraceus and penicillium verrucosum. in argentina, there is no available information about the natural occurrence of oa and ochratoxigenic fungi from feedstuffs. the aim was to evaluate the natural occurrence of oa in poultry, pig and rabbit feeds over 8 months. likewise, the capacity to produce oa by aspergillus section ... | 2002 | 12456278 |
| nigerloxin, a novel inhibitor of aldose reductase and lipoxygenase with free radical scavenging activity from aspergillus niger cfr-w-105. | an enzyme inhibitor, nigerloxin, with inhibition against soy bean lipoxygenase-i (lox-1), rat lens aldose reductase (rlar) as well as free radical scavenging activity was isolated from the fermented wheat bran using aspergillus niger cfr-w-105. its chemical structure was identified as 2-amido-3-hydroxy-6-methoxy-5-methyl-4-(prop-1'-enyl) benzoic acid by nmr and gceims data. the ic50 values against lox-1 and rlar were found to be 79 microm and 69 microm and ed50 against 1,1-diphenyl-2-picrylhydra ... | 2002 | 12458767 |
| electrical contacting of flavoenzymes and nad(p)+-dependent enzymes by reconstitution and affinity interactions on phenylboronic acid monolayers associated with au-electrodes. | the preparation of integrated, electrically contacted, flavoenzyme and nad(p)(+)-dependent enzyme-electrodes is described. the reconstitution of apo-glucose oxidase, apo-gox, on a fad cofactor linked to a pyrroloquinoline quinone (pqq) phenylboronic acid monolayer yields an electrically contacted enzyme monolayer (surface coverage 2.1 x 10(-)(12) mol cm(-)(2)) exhibiting a turnover rate of 700 s(-)(1) (at 22 +/- 2 degrees c). the system is characterized by microgravimetric quartz-crystal microba ... | 2002 | 12465985 |
| bioprocessing strategies for improving hen egg-white lysozyme (hewl) production by recombinant aspergillus niger hewl wt-13-16. | hen egg-white lysozyme (hewl) production by recombinant aspergillus niger hewl wt-13-16 from a cdna under the control of the a. niger glucoamylase promoter was used as a model system. the fungal mycelium was either immobilized on porous celite 560 micro-carrier or grown in suspension as pelleted and dispersed forms. the objective was to reduce the protease activity that adversely affects the expressed hewl. free suspension culture at uncontrolled ph served as the benchmark. the control of ph dur ... | 2002 | 12466879 |
| mono- and dimeric ferulic acid release from brewer's spent grain by fungal feruloyl esterases. | ultraflo l, a beta-glucanase preparation from humicola insolens sold for reducing viscosity problems in the brewing industry, exhibited activity against the methyl esters of ferulic, caffeic, p-coumaric and sinapic acids, displaying mainly type-b feruloyl esterase activity. ultraflo also contained the ability to release 65% of the available ferulic acid (fa) together with three forms of diferulate from brewer's spent grain (bsg). an "esterase-free" ultraflo preparation greatly enhanced the abili ... | 2002 | 12466893 |
| brainstem auditory evoked response in adolescents with acoustic mycotic neuroma due to environmental exposure to toxic molds. | indoor air contamination with toxic opportunistic molds is an emerging health risk worldwide. some of the opportunistic molds include: stachybotrys chartarum, aspergillus species (a. fumigatus, a. flavus, a. niger, a. versicolor etc.), cadosporium, alternaria, penicillium, trichoderma, fusarium graminearum etc. these molds flourish in homes that are moist and damp. reports of floods are now evident in many parts of the world. with these global changes in climatic conditions that favor the opport ... | 2002 | 12467209 |
| genetic programming assisted stochastic optimization strategies for optimization of glucose to gluconic acid fermentation. | this article presents two hybrid strategies for the modeling and optimization of the glucose to gluconic acid batch bioprocess. in the hybrid approaches, first a novel artificial intelligence formalism, namely, genetic programming (gp), is used to develop a process model solely from the historic process input-output data. in the next step, the input space of the gp-based model, representing process operating conditions, is optimized using two stochastic optimization (so) formalisms, viz., geneti ... | 2002 | 12467472 |
| synthesis and antifungal activity of 2,5-disubstituted-6-arylamino-4,7-benzimidazolediones. | 2,5-disubstituted-6-arylamino-4,7-benzimidazolediones were synthesized and tested for in vitro antifungal activity against pathogenic fungi. among them, 6-arylamino-5-chloro-2-(2-pyridyl)-4,7-benzimidazolediones exhibited potent antifungal activity against candida species and aspergillus niger. | 2003 | 12467608 |
| cloning, nucleotide sequencing, and expression of the beta-galactosidase-encoding gene (laca) from aspergillus oryzae. | laca coding for beta-galactosidase (beta-gal) was cloned from the genomic dna of aspergillus oryzae rib40. there were 9 exons in laca and the coding region of 3,015 bp encoded a protein of 1,005 aa with a deduced molecular mass of 109,898. a. oryzae laca was highly homologous to fungal beta-gals, with the highest aa identity of 70.7% to a. niger laca, and also showed significant identity to acid beta-gals belonging to family 35 glycosyl hydrolases. approximately 10 copies of laca under control o ... | 2002 | 12469296 |
| comparison of in vitro antifungal activities of amphotericin b lipid complex with itraconazole against 708 clinical yeast isolates and opportunistic moulds determined by national committee for clinical laboratory standards methods m27-a and m38-p. | we compared the in vitro antifungal activity of amphotericin b lipid complex (ablc) with that of itraconazole (itz) against 535 yeast strains and 173 opportunistic filamentous fungi by using a microdilution method (national committee for clinical laboratory standards m27-a and m38-p). the overall geometric mean mic was 0.13 microg/ml and 0.177 microg/ml for itz and ablc, respectively, and the mic(50) was 0.125 microg/ml for both agents against yeast isolates. itz had a similar or slightly superi ... | 2002 | 12476038 |
| the influence of metal ions on malic enzyme activity and lipid synthesis in aspergillus niger. | in the presence of copper significant induction of citric acid overflow was observed, while concomitantly lower levels of total lipids were detected in the cells. its effect was more obvious in a medium with magnesium as sole divalent metal ions, while in a medium with magnesium and manganese the addition of copper had a less pronounced effect. since the malic enzyme was recognised as a supplier of reducing power in the form of reduced nicotinamide adenine dinucleotide phosphate for lipid biosyn ... | 2002 | 12480102 |
| regulation of the amylolytic and (hemi-)cellulolytic genes in aspergilli. | filamentous fungi produce high levels of polysaccharide-degrading enzymes and are frequently used for the production of industrial enzymes. because of the high secretory capacity for enzymes, filamentous fungi are effective hosts for the production of foreign proteins. genetic studies with aspergillus nidulans have shown pathway-specific regulatory systems that control a set of genes that must be expressed to catabolize particular substrates. besides the pathway-specific regulation, wide domain ... | 2001 | 12483563 |
| strain improvement of aspergillus niger for enhanced lipase production. | the enhancement of lipase production from aspergillus niger was attempted by ultraviolet (uv) and nitrous acid mutagenesis, and the mutants were selected on media containing bile salts. nitrous acid mutants exhibited increased efficiency for lipase production when compared with uv mutants in submerged fermentation. the hyperproducing uv and nitrous acid mutants were further subjected to a second step of mutagenesis to devise an economical and ecofriendly technique for lipase production by the ef ... | 2001 | 12483617 |
| efficacy of antimicrobial filter treatments on microbial colonization of air panel filters. | to assess the activity of biostatic agents on the microbial colonization of panel filters. | 2003 | 12492918 |
| characterisation of the gpta gene, encoding udp n-acetylglucosamine: dolichol phosphate n-acetylglucosaminylphosphoryl transferase, from the filamentous fungus, aspergillus niger. | the production of asparagine (n)-linked oligosaccharides is of vital importance in the formation of glycosylated proteins in eukaryotes and is mediated by the dolichol pathway. as part of studies to allow manipulation of this pathway, the gene coding for the production of the enzyme udp n-acetylglucosamine: dolichol phosphate n-acetylglucosaminylphosphoryl transferase (gpt), catalysing the first step in the assembly of dolichol-linked oligosaccharides, was cloned from the filamentous fungus aspe ... | 2003 | 12495819 |
| biosorption and solubilization of copper oxychloride fungicide by aspergillus niger and the influence of calcium. | the biosorption of copper oxychloride fungicide particulates (approximately 1 microm diameter), at concentrations ranging from 25 to 500 ppm active ingredient (ai), by pelleted mycelium of aspergillus niger grown on czapek dox medium was evaluated. the concentration of the fungicide adsorbed to the mycelium, remaining suspended or solubilized in the medium, was determined by analysis of its copper content (cuf) using atomic absorption spectrophotometry (aas). 2-day-old pellets exhibited high bio ... | 2002 | 12498216 |
| exposure to airborne microorganisms and endotoxin in a potato processing plant. | microbiological air sampling was performed in a big potato processing plant located in eastern poland. air samples for determination of concentrations of microorganisms, dust and endotoxin were collected at 6 sites in the division producing potato flakes and meal from dried potato pulp and at 2 sites in the division producing potato syrup from imported starch. the concentrations of total airborne microorganisms were within a range of 28.3-93.1 x 10(3) cfu/m(3). mesophilic bacteria were dominant ... | 2002 | 12498592 |
| overproduction of alpha-glucosidase in aspergillus niger transformed with the cloned gene agla. | we have transformed an industrial strain, aspergillus niger gn-3, with the alpha-glucosidase gene (agla) from the same strain. southern hybridization analysis revealed that transformants had multiple copies of the cloned dna inserted into the host genome. an 11-fold improvement of enzyme production was achieved by transformation with a dna fragment composed of 1.11 kb of the 5' noncoding region, 3.12 kb of the coding region containing three introns, and 1.2 kb of the 3' noncoding region. it was ... | 1998 | 12501426 |
| five new steroidal saponins from solanum chrysotrichum leaves and their antimycotic activity. | using bioactivity-directed isolation procedures, five new spirostan saponins and two sterol glycosides have been isolated from solanum chrysotrichum leaves. the structure of these compounds was established based upon spectroscopic measurements, especially 1d and 2d nmr data of their peracetate derivatives. these compounds showed antimycotic activity. the most active compound is 6alpha-o-beta-d-xylopyranosyl-(1-->3)-beta-d-quinovopyranosyl-(25r)-5alpha-spirostan-3beta,23alpha-ol (2) (mic =12.5, 1 ... | 2002 | 12502320 |
| influence of cultivation conditions on citrate production by aspergillus niger in a semi-pilot-scale plant. | the influence of some fermentation parameters on the semi-pilot scale (alteration of growth conditions, e.g., sugar concentration, incubation temperature and initial ph) on citrate production was demonstrated in parent and mutant strains of aspergillus niger. raw material from sugar industry (cane molasses) was examined as basal fermentation medium in a stirred stainless-steel 15-l fermentor. after growth on medium with 150 g/l sugar, the parent strain produced 51.2 g/l citric acid; the mutant s ... | 2002 | 12503396 |
| catalysis of electron transfer during activation of o2 by the flavoprotein glucose oxidase. | two prototropic forms of glucose oxidase undergo aerobic oxidation reactions that convert fadh(-) to fad and form h(2)o(2) as a product. limiting rate constants of k(cat)k(m)(o(2)) = (5.7 +/- 1.8) x 10(2) m(-1).s(-1) and k(cat)k(m)(o(2)) = (1.5 +/- 0.3) x 10(6) m(-1).s(-1) are observed at high and low ph, respectively. reactions exhibit oxygen-18 kinetic isotope effects but no solvent kinetic isotope effects, consistent with mechanisms of rate-limiting electron transfer from flavin to o(2). site ... | 2003 | 12506204 |
| antimicrobial properties of highly fluorinated bis-ammonium salts. | four series of new highly fluorinated bisammoniums (quaterfluo tx, quaterfluo bx, quaterfluo cx, quaterfluo dx) were tested to evaluate their anti-bacterial and anti-fungal properties. four microbial strains were used to perform the study: pseudomonas aeruginosa, staphylococcus aureus, candida albicans and aspergillus niger. the antimicrobial efficacy was measured by bacterial and fungal growth inhibition expressed as minimal inhibitory concentration (mic) values. the quaterfluo tx series had ve ... | 2003 | 12507834 |
| synthesis and antifungal activity of 6,7-bis-[s-(aryl)thio]-5,8-quinolinediones. | 6,7-bis-[s-(aryl)thio]-5,8-quinolinediones 4 and 5 were synthesized by the substitution of 6,7-dichloro-5,8-quinolinediones with appropriate arylthiols. their antifungal activity were tested in vitro for their growth inhibitory activities against pathogenic fungi in comparison with flucytosine. the antifungal activities were significantly improved by s-(aryl)thio moieties of the compounds 4 and 5. the all tested compounds 4 and 5 showed generally good activities against c. albicans and a. niger ... | 2002 | 12510828 |
| trends in contact lens-associated microbial keratitis in southern india. | to review the microbiologic profile, clinical course, treatment, and outcome in patients with contact lens-associated microbial keratitis in the setting of a tertiary eye care center in a developing country in the tropics. | 2003 | 12511359 |
| [observation of cell ultrastructuse in suppurative otitis media treated with bosneol and application]. | to explore the pharmacological action and optimal concentration of borneol in treating suppurative otitis media. | 2000 | 12512458 |
| acetic acid increases stability of silage under aerobic conditions. | the effects of various compounds on the aerobic stability of silages were evaluated. it has been observed that inoculation of whole-crop maize with homofermentative lactic acid bacteria leads to silages which have low stability against aerobic deterioration, while inoculation with heterofermentative lactic acid bacteria, such as lactobacillus brevis or lactobacillus buchneri, increases stability. acetic acid has been proven to be the sole substance responsible for the increased aerobic stability ... | 2003 | 12514042 |
| [fungal keratitis at the centre hospitalier national d'ophtalmologie des quinze-vingts: retrospective study of 19 cases]. | to report the spectrum of fungal keratitis at the centre hospitalier national d'ophtalmologie xv-xx, paris. | 2002 | 12515932 |
| evaluation of broth microdilution testing parameters and agar diffusion etest procedure for testing susceptibilities of aspergillus spp. to caspofungin acetate (mk-0991). | the nccls m38-a document does not describe guidelines for testing caspofungin acetate (mk-0991) and other echinocandins against molds. this study evaluated the susceptibilities of 200 isolates of aspergillus fumigatus, a. flavus, a. nidulans, a. niger, and a. terreus to caspofungin (mics and minimum effective concentrations [mecs]) by using standard rpmi 1640 (rpmi) and antibiotic medium 3 (m3), two inoculum sizes (10(3) and 10(4) cfu/ml), and two mic determination criteria (complete [mics-0] an ... | 2003 | 12517880 |
| concomitant otomycosis and dermatomycoses: a clinical and microbiological study. | otomycosis tends to recur despite long-term treatment. to our knowledge, there is no study in the english literature concerning the clinical importance of concomitant otomycosis and dermatomycoses. we investigated the presence of dermatomycoses in 52 patients with otomycosis in order to document the clinical and microbiological importance of their coexistence. dermatomycoses on the feet and/or hands were identified in 19 patients (36.5%). the most common pathogen for otomycosis was aspergillus n ... | 2003 | 12520352 |
| usefulness of a colorimetric method for testing antifungal drug susceptibilities of aspergillus species to voriconazole. | the usefulness of a colorimetric method using alamar blue (alamar blue method) for susceptibility testing of aspergillus species to voriconazole and three existing antifungal agents, itraconazole (itcz), flucytosine (5-fc), and amphotericin b (amph-b), was studied using four atcc strains of three species, including two strains of a. fumigatus and one each of a. flavus and a. niger. for comparison, the broth microdilution method for antifungal susceptibility testing of filamentous fungi proposed ... | 2002 | 12525904 |
| [separation and purification of cellulase using affinity membrane]. | the importance of cellulase as a means for the efficient utilization of abundant cellulose resources in the world has been well recognized. many researchers devote themselves to studying the mechanism of the action of cellulase to cellulose so that such expensive enzyme can be used much more widely. the first step is to obtain cellulase of high purity. so purification of cellulase is the key point in this field. however, the major problem in isolation is that cellulase is a complicated enzyme sy ... | 2002 | 12541911 |
| new dimeric naphthopyrones from aspergillus niger. | three new dimeric naphthopyrones, asperpyrones a (1), b (2), and c (3), together with two known compounds, fonsecinone a (4) and aurasperone a (5), have been isolated from okara that was fermented with aspergillus niger jv-33-48. compounds 1, 4, and 5 showed inhibitory activity on taq dna polymerase. | 2003 | 12542363 |
| endophthalmitis in patients with disseminated fungal disease. | fungal endophthalmitis caused by dissemination from extraocular fungal infections has been reported to vary between 9% and 45%. however, recent clinical experience disagrees with that. this study is an investigation of patients in an inner city teaching hospital, the risks associated with endogenous fungal endophthalmitis, and this incidence. | 2002 | 12545679 |
| [isolation and characterization of mutants of aspergillus niger deficient in extracellular proteases]. | several mutants of aspergillus niger 3.795, deficient in extracellular protease expression, have been isolated and characterized biochemically after in vitro uv-mutagenesis of conidiospores and selected by halo-screening on the gelatin/casein plate and skim milk plate. extracellular proteolytic activities of 3.795-1-23 and 3.795-1-30 are 5.4% and 8.4% of the parental strain and these strains may be used as the host for efficient expression of foreign proteins. | 2000 | 12548962 |
| [the intergeneric compatibility of heredity and expression for cellulase genomes between aspergillus niger and trichoderma reesei]. | by using the developed display techniques of cellulase isozymes and the rapd-pcr analysis guided by a deduced universal sequence of cellulase genes, the polymorphisms of genomic dna fingerprints and cellulase isozymes were compared among three typical stable recombinants (3a, 3b, a7-1) and their two parents (aspergillus niger ams11, trichoderma reesei qm9414) in order to provide the molecular evidence of gene recombination, to demonstrate the compatibility of heredity and expression of intergene ... | 1998 | 12549330 |
| [studies on reduction of hexavalent chromium by fungi]. | several fungal strain, isolated from activated sludge, sewage and soil, were able to grow in the medium containing 300-500 mg/l k2cr2o7, and strain bs-1 was capable of resistance to k2cr2o7 up to 900 mg/l. after 4-6 d growing of the strain bs-1 and other 3 strains in the liquid medium added 200 mg/l k2cr2o7, the cr(vi) content of the medium disappeared completely. these strain were identified as penicillium sp. bs-1, bs-3, aspergillus niger br-4, aspergillus flavus bx-1 respectively. the results ... | 1998 | 12549370 |
| [purification and properties of inulinases from aspergillus niger m89]. | four inulinase components were purified from aspergillus niger m89 by (nh4)2so4 fractionation, sephadex g-200 gel filtration, deae-cellulose chromatography and polyacrylamide gel electrophoresis (page). the molecular weight of ei eii eiii and eiv were indicated to be 102.6, 97.9, 62.5 and 36.5 kd respectively by sds-page. measured by isoeleetric focusing, their isoelectric points were 4.15, 4.24, 4.48 and 4.15 respectively. four forms of inulinase exibited maximal inulinase activity between 55-6 ... | 1998 | 12549372 |
| [research on the regulation of glucoamylase gene(glaa) expression in a. niger. ii. analysis of the function of 5'-regulatory region of a. niger t21 and 3.795 glaa gene]. | two plasmid vectors pxh2 and pgh1 were constructed through the fusion of e. coli hph gene, the report gene and the 5' upstream regions of a. niger t21 and 3.795 respectively, as well as the terminator of a. nidulans trpc gene. the plasmid vectors were than used to transform a. niger t21 to functionally identify those different basic groups between the two 5' upstream regions responsible for high-level expression of the glaa gene. southern analysis of two transformants xh2c and gh1c revealed that ... | 1998 | 12549385 |
| [studies on microbial factor on color change of dunhuang mural. i. classification of microbes on color-changed mural and property of some typical species]. | microbial strains were isolated from 51 typical color changed samples of mural in 6 grottos of dunhuang mogao gretto. after identification, it is proved that belong to 6 genera of bacteria and 5 genera of mould. bacillus, alcaligenes and penicillium were dominant species in all of them. in the imitative experiment, it is found that cladosporium sp., a. niger and two strains of bacteria have a great effect on the color change of red pigment of mural and aging of sizing agent. | 1998 | 12549389 |
| [screening and breeding of highly-effected degrading cotton-phenol strains and study on defoxication technology and conditions]. | from mildewed cottonseed cake and stock cultures of mold and yeast. we select more than ten strains of yeasts and molds which can degrade cotton phenol. at last we got four strains which can degrade cotton phenol highly effected after mutagenized by physical and chemical factors and induced by cotton phenol. they belong to candida tropicalis, torulopsis candida, aspergillus flavus and aspergillus niger. by small and medium size fermenfations, the content of dissociated cotton phenol all reach sa ... | 1998 | 12549423 |
| heavy atom isotope effects on the reaction catalyzed by the oxalate decarboxylase from bacillus subtilis. | oxalate decarboxylase (oxdc) catalyzes a remarkable transformation in which the c-c bond in oxalate is cleaved to give carbon dioxide and formate. like the native oxdc isolated from aspergillus niger, the recombinant, bacterial oxdc from bacillus subtilis contains mn(ii) in its resting state and requires catalytic dioxygen for activity. the most likely mechanism for oxdc-catalyzed c-c bond cleavage involves the participation of free radical intermediates, although this hypothesis remains to be u ... | 2003 | 12553826 |
| enhanced settleability and dewaterability of fungal treated domestic wastewater sludge by liquid state bioconversion process. | a study was conducted to evaluate the settleability and dewaterability of fungal treated and untreated sludge using liquid state bioconversion process. the fungal mixed culture of aspergillus niger and penicillium corylophilum was used for fungal pretreatment of wastewater sludge. the fungal strains immobilized/entrapped on sludge particles with the formation of pellets and enhanced the separation process. the results presented in this study showed that the sludge particles (pellets) size of 2-5 ... | 2003 | 12553987 |
| [screening of acidic xylanase producing strain and studies on its enzyme production conditions]. | from 150 fungal strains, the authors found 8 strains contained mainly of xylanase activity over 100 u/ml in which the no. 149 strain was the highest xylanase producer. which tentatively identified as aspergillas niger. the appropriate medium composition was as follows: wheat bran hemicellulose 4%; nano3 1%; wheat bran 1% prepared in mandels nutritional solution without (nh4)2so4 and urea. after cultivated in shake-flask at 28 degrees c-32 degrees c for 60 h, the activity reached the highest valu ... | 1999 | 12555575 |
| improvement of growth and nutritive value in chicks with non-genetically modified phytase product from aspergillus niger. | 1. non-genetically modified (non-gm) phytase product derived from aspergillus niger possesses various side active enzymes including alpha-amylase, protease, cellulase and hemicellulase. in contrast, the product of genetically modified (gm) phytase product has much less side active enzyme since the capacity of phytase production is reinforced by gene modification. in the present study we have tried to determine whether the difference of side enzyme activity of phytase product affects growth perfo ... | 2002 | 12555893 |
| [screening strains producing inulinase and cloning of inulinase gene]. | a wild-type strain af10 producing inulinase was screened from soil samples, the strain is identified to be a a. niger. a endoinulinase gene inua1 from a. niger af10 was wequenced and analyzed, after pcr amplification. the results shows inua1 is 1551 bp in length without any intron and contains 4 potential n-glycosylation sites. the conservative sequence is wmnepn from the n-terminus. the inua1 was cloned to puc118 and the recombinant vector pinua1 was obtained and transformed into e. coli jm109. ... | 2002 | 12557373 |
| testing the limits of biological tolerance to arsenic in a fungus isolated from the river tinto. | the tinto river in spain, with its high acidity and heavy metal concentrations (as, cu, cr, zn), is an example of an environment hostile to life. yet despite these extreme conditions, the site possesses a great diversity of eukaryotic life forms. we report the isolation of a filamentous fungus able to grow at 200 mm arsenic ( approximately 15 000 p.p.m.), i.e. a concentration 20-fold above that withstood by the reference microorganisms escherichia coli, saccharomyces cerevisiae and aspergillus n ... | 2003 | 12558596 |
| [characterization of inductive synthesis of levoglucosan kinase by a combined strategy of enzymological and fast atom bombardment mass spectrometric analysis]. | levoglucosan is the main product derived from pyrolysis of cellulose. a mutant aspergillus niger cbx-209 could grow on levoglucosan well fermenting it into citric acid with a yield comparable to that on glucose. levoglucosan hydrolase was absent by measuring glucose formation with the glucose oxidase and peroxidase coupling system. cell extracts were partly purified by ammonium sulfate fractionation and ion-exchange chromatograph. direct formation of glucose 6-phosphate from levoglucosan in the ... | 2002 | 12561203 |
| [cloning and sequence analysis of the phytase phya gene of aspergillus niger n25]. | the phya encoding phytase of aspergillus niger n25 was amplified by the polymerase chain reaction (pcr) with primers designed according to the sequences of the phya in genbank. the amplified fragment was cloned and sequenced. the results show that: the coding region is 1506 bp in size, includes a 102 bp intron, and encodes a peptide of 476 amino acid residues, in which there is a signal peptide with 19 amino acids and a mature peptide of 448 amino acids. comparison of this sequence with the phya ... | 2001 | 12561778 |
| isolation of fungi in musca domestica linnaeus, 1758 (diptera: muscidae) captured at two natural breeding grounds in the municipality of serop dica, rio de janeiro, brazil. | the objective of this study was to isolate and identify fungal species found in natural association with adults of musca domestica. the adult insects were collected from two natural breeding grounds: hog pens and an urban sanitary landfill. the isolated fungi were identified as: aspergillus flavus (23.8%), a. niger var. niger (14.4%), penicillium corylophilum (21.4%), p. fellutanum (11.9%), cladosporium cladosporoides (4.7%), fusarium sp. (4.7%), alternaria alternata (11.9%), curvularia brachysp ... | 2003 | 12563474 |
| synthesis of antioxidant propyl gallate using tannase from aspergillus niger van teighem in nonaqueous media. | tannase from aspergillus niger van teighem has been used for synthesis of food additive antioxidant propyl gallate by direct transesterification of tannic acid. the optimized yield of 86% was obtained by using simultaneously ph tuned enzyme, immobilized on celite and using the right amount of water in the non aqueous media. | 2003 | 12565937 |
| essential oil composition and antimicrobial activity of santiria trimera bark. | the composition and the antimicrobial activity of the bark oil of santiria trimera (oliv.) aubrév., a plant widely used by the traditional healers in s. tomé and príncipe, especially for wound healing, are reported for the first time. the analysis of the essential oil was carried out by gc and gc-ms. the oil contains a high content of monoterpenes, alpha-pinene (66.6 %) being the major constituent, followed by beta-pinene (20.0 %). the essential oil was active against both bacteria and fungi str ... | 2003 | 12567287 |
| the source of ochratoxin a in brazilian coffee and its formation in relation to processing methods. | a total of 408 brazilian coffee samples was examined during the 1999 and 2000 coffee harvest seasons for the presence of ochratoxin a (oa) and fungi with the potential to produce it. samples came from four regions: alta paulista (western area of são paulo state), sorocabana (southwest são paulo state), alta mogiana (northeast são paulo state) and cerrado mineiro (western area of minas gerais state). cherries and beans were examined at different stages: immature, mature and overripe cherries from ... | 2003 | 12568757 |
| aminopeptidase c of aspergillus niger is a novel phenylalanine aminopeptidase. | a novel enzyme with a specific phenylalanine aminopeptidase activity (apsc) from aspergillus niger (cbs 120.49) has been characterized. the derived amino acid sequence is not similar to any previously characterized aminopeptidase sequence but does share similarity with some mammalian acyl-peptide hydrolase sequences. apsc was found to be most active towards phenylalanine beta-naphthylamide (f-beta na) and phenylalanine para-nitroanilide (f-pna), but it also displayed activity towards other amino ... | 2003 | 12571053 |
| [improvement of product technology on prepared soypean]. | this paper reports the new prepared method of prepared soypean by purebred culture of aspergillus niger. the technology condition is that temperture is 28 +/- 2 degrees c and comparative humidity is 95%, time of fermented culture is 15-20 days. after improvement, polluttion of other bacteria is easily avoided. | 1997 | 12572415 |
| extent of fungal growth on fiberglass duct liners with and without biocides under challenging environmental conditions. | eight brands of fiberglass duct liners, including three that contained biocides, were exposed to challenging environmental conditions that would promote fungal growth. twenty-four rectangular sheet metal ducts in three groups of eight ducts per group were lined with the eight selected liners. each group of ducts was exposed to one of the three test conditions within an environmental chamber for a period of 15 days. these conditions were a) 75 percent rh, b) 75 percent rh plus water spray, c) 75 ... | 2003 | 12573965 |
| pcr assay for identification of histoplasma capsulatum based on the nucleotide sequence of the m antigen. | the major diagnostic antigens of histoplasma capsulatum var. capsulatum are the h and m antigens, pluripotent glycoproteins that elicit both humoral and t-cell-mediated immune responses. the gene encoding the m antigen has previously been sequenced, and its sequence has significant overall homology to those of the genes for fungal catalases. regions of the m-antigen gene with little or no homology were used to design four oligonucleotide sequences for application in the pcr detection and identif ... | 2003 | 12574242 |
| detection and identification of fungi from fungus balls of the maxillary sinus by molecular techniques. | the aim of this study was to find a reliable method for the detection and identification of fungi in fungus balls of the maxillary sinus and to evaluate the spectrum of fungi in these samples. one hundred twelve samples were obtained from patients with histologically proven fungal infections; 81 samples were paraffin-embedded tissue sections of the maxillary sinus. in 31 cases, sinus contents without paraffin embedding were sent for investigation. pcr amplification with universal fungal primers ... | 2003 | 12574250 |
| examining the role of glutamic acid 183 in chloroperoxidase catalysis. | site-directed mutagenesis has been used to investigate the role of glutamic acid 183 in chloroperoxidase catalysis. based on the x-ray crystallographic structure of chloroperoxidase, glu-183 is postulated to function on distal side of the heme prosthetic group as an acid-base catalyst in facilitating the reaction between the peroxidase and hydrogen peroxide with the formation of compound i. in contrast, the other members of the heme peroxidase family use a histidine residue in this role. plasmid ... | 2003 | 12576477 |
| synthesis and biological activities of 2,6-diaryl-3-methyl-4-piperidone derivatives. | in the present study, a new series of 2,6-diaryl-3-methyl-4-piperidones was synthesized by mannich reaction (condensation) of ethyl-methyl ketone, substituted aromatic aldehydes and ammonium acetate. oximes and thiosemicarbazone derivatives of 2,6-diaryl-3-methyl-4-piperidones were synthesized by reaction with hydroxylamine hydrochloride and thiosemicarbazide respectively. the chemical structures were confirmed by means of ir, 1h-, 13c-nmr and mass spectral data. the compounds were screened for ... | 2003 | 12576678 |
| [chemical constituents of diuranthera inarticulata]. | to investigate the chemical constituents from the roots of diuranthera inarticulata wang et k. y. lang. | 2001 | 12579935 |
| use of cas-agar plate modified to study the effect of different variables on the siderophore production by aspergillus. | to evaluate the suitability of chrome azurol s (cas) agar plate assay as a quantitative methodology for siderophore production. | 2003 | 12581379 |
| transcriptional regulation of xyn2 in hypocrea jecorina. | the xylanase system of the filamentous fungus hypocrea jecorina (trichoderma reesei) consists of two specific xylanases, xyn1 and xyn2, which are simultaneously expressed during growth on xylan but respond differentially to low-molecular-weight inducers. using in vivo footprinting analysis of xylan-induced and noninduced mycelia, we detected two adjacent nucleotide sequences (5'-agaa-3' on the noncoding strand and 5'-gggtaaattgg-3', referred to as the xylanase-activating element [xae], on the co ... | 2003 | 12582132 |