| oxalosis associated with an aspergillus niger fungus ball. report of a case. | during a seventeen day period an a. niger fungus ball evolved within a healed tuberculous cavity of a patient. symptoms were a cough with a chocolate brown expectoration and dyspnea. the patient died and necropsy was performed. crystals of calcium oxalate were deposited in the cavity lining and in the adjacent tissue of the lung. fibrosis, mononuclear infiltration and intraalveolar purulent exudate were seen in these tissues. some small vessels presented recent thrombosis and deposition of calci ... | 1981 | 7219509 |
| leaching of a silicate and carbonate copper ore with heterotrophic fungi and bacteria, producing organic acids. | leaching of metals with carbon-heterotrophic microorganisms and organic acids as active agents has been performed with timna copper ore containing 1.1% copper as carbonates and silicate and with some other metal oxides. aspergillus niger surface cultures on a 14% sucrose fermentation medium yeilded 87.3% of total copper from 10% suspension within 2 weeks of leaching. on sulfite liquor as an industrial waste product a. niger solubilized 73.8% cu within 4 weeks. in percolators with 14% sucrose med ... | 1980 | 7222743 |
| [effect of local anesthetics on the transport of mannose in the microsomal membranes of aspergillus niger van tieghem]. | the influence of various local anaesthetics has been studied on the mannose transfer enzymic system which is localized in aspergillus niger van tieghem microsomal membranes. the n-alkanes and tertiary amines do not seem to react on the first step of the reaction: i.e. the polyprenylphosphate-mannose biosynthesis. however two amines, the dibucaine and to a lesser extent the tetracaine, inhibit the mannose transfer from the polyprenylphosphate-mannose to the endogenous proteins. | 1981 | 7226275 |
| increased respiratory activity in a diploid strain of aspergillus niger (v35). | | 1981 | 7237283 |
| mode of elongation of the glycerol phosphate polymer of membrane lipoteichoic acid of streptococcus faecium atcc 9790. | specific degradation of membrane lipoteichoic acid of streptococcus faecium atcc 9790 by a phosphodiesterase from aspergillus niger and by periodate oxidation has demonstrated that the enzymatic synthesis of the glycerol phosphate polymer of the molecule occurs by an external elongation system. evidence of this type of mechanism was obtained with lipoteichoic acid synthesized in vivo or in vitro by differential radioisotope labeling techniques. the glycerol phosphate repeating units were transfe ... | 1981 | 7240097 |
| [effect of composition of multienzyme cellulase complexes on rate-limiting steps for non-soluble (native) cellulose hydrolysis]. | it was shown that the kinetics of multienzyme cellulase complexes are generally characterized by the absence of any single rate-limiting step of hydrolysis, even when the rates of individual steps of the process are substantially different. this peculiarity of the kinetic behaviour of cellulases can be explained in terms of certain "shunt ways" in a series of individual steps of enzymatic degradation of cellulose into glucose. a basic kinetic scheme for the degradation of cellulose was developed ... | 1981 | 7248368 |
| [about the antimicrobial activity of substituted aromatic aldehydes (author's transl)]. | the mic-values and the antimicrobial activity in the paper disc diffusion method of 86 resp. 79 substituted aromatic aldehydes have been investigated. antibacterial activity has been tested against staphylococcus aureus, pseudomonas aeruginosa, proteus vulgaris and klebsiella pneumoniae, the antifungal activity against trichophyton mentagrophytes, aspergillus niger and candida albicans. 13 classes of substituents have been investigated. the most active compounds are substituted with nitro-, hydr ... | 1981 | 7257654 |
| isolation, purification and some chemical properties of an acid carboxypeptidase from aspergillus niger var. macrosporus. | acid carboxypeptidase (peptidyl-l-amino-acid hydrolase, ec 3.4.16.1) was purified to a homogeneous state from the water extracts of koji cultures of aspergillus niger var. macrosporus. the molecular weight of the enzyme was determined to e 136 000 by sedimentation equilibrium method. the denatured specimen of the enzyme exhibited a molecular weight of 60 000 in the sedimentation equilibrium in 6 m guanidinium chloride, suggesting that the native enzyme is composed of two identical subunits. howe ... | 1981 | 7260098 |
| studies on air-borne fungi at qena. i. seasonal fluctuations. | 73 species which belong to 24 genera were collected in 200 and 35 exposures made during the period may 1976-october 1977 at each of two levels (2 m and 20 m). the air-borne fungi showed seasonal periodicities and the highest incidence was recovered in spring and autumn and the least in the summer. aspergillus was the dominating genus. 17 species were collected at the two levels of which a. niger and a. flavus were the most common. other common genera were cladosporium which was represented by c. ... | 1981 | 7269656 |
| [characterization of the cleaving mechanism of pectinesterase of aspergillus niger]. | | 1981 | 7278946 |
| aspergillus niger. | | 1981 | 7293976 |
| the role of oligosaccharide in transport of egg yolk riboflavin-binding protein to the egg. | the carbohydrate portion of chicken egg yolk riboflavin-binding protein was examined to determine its role in the biological activity of the protein. yolk rbp was found to contain 5--6 mannose, five galactose, 12 n-acetylglucosamine and four sialic acid residues. specific modifications of the oligosaccharide moiety were performed which included removal of sialic acid by mild acid hydrolysis, oxidation of galactose by galactose oxidase, and removal of n-acetylglucosamine and galactose residues by ... | 1981 | 7295796 |
| electrostatic control of enzyme reactions: effect of ionic strength on the pka of an essential acidic group on glucose oxidase. | the dissociation constant of an essential acidic group on the reduced form of glucose oxidase from aspergillus niger (k4) has been found to be extremely sensitive to ionic strength. increasing the ionic strength from 0.025 to 0.225 causes a decrease in pk4,obsd of 0.9 ph unit, from 8.2 to 7.3. analysis of the ionic strength dependence of pk4,obsd, making the assumption that the enzyme is a homogeneously charged impenetrable sphere [edsall, j. t., & wyman, j. (1958) biophysical chemistry, vol. 1, ... | 1981 | 7317376 |
| purification and properties of aldose 1-epimerase from aspergillus niger. | aspergillus niger atcc 6274 was selected as an aldose 1-epimerase (ec 5.1.3.3) producer from 45 stock cultures of a. niger. the aldose 1-epimerase was purified 115-fold to apparent homogeneity from cell extracts with a yield of 2.6%. the molecular weight was calculated to be 260,000 and that of the subunit to be 130,000. the enzyme preparation was active at ph 5-7. the km value was 50 mm and the v value was 1200 units/mg toward alpha-d-glucose. this enzyme catalyzed mutarotation of the following ... | 1981 | 7317442 |
| [comparative physiological and biochemical characteristics of aspergillus niger isolated from the mesosphere and of its mutant]. | the aim of this work was to study certain physiological-biochemical characteristics of aspergillus niger, strain 26, isolated from the mesosphere as well as those of its mutant having light-brown conidia. the parent strain and its mutant were grown in a liquid chapek medium to study accumulation of the biomass, changes in the ph of the medium, as well as assimilation of glucose, nitrogen (no3-) and phosphorus (po4-). the content of polysaccharides, protein, rna and dna was determined in the biom ... | 1981 | 7329352 |
| bilateral endogenous aspergillus endophthalmitis. | a case of successfully treated aspergillus endophthalmitis is presented. treatment consisted of wide vitrectomy. at the end of surgery, 5 micrograms of amphoteracin b were injected into the center of the vitreous cavity, and an additional .75 mg was given subconjunctivally both at surgery and on the second postoperative day, at which time 2 mg of dexamethasone were also given subjunctivally. systemic antifungal agents were not used. after 2 1/2 months, the second eye developed a similar metastat ... | 1981 | 7348830 |
| metabolite in diagnosis of pulmonary aspergillosis and oxalic acid factor. | | 1980 | 7352707 |
| chelating, film-forming, and coagulating ability of the chitosan-glucan complex from aspergillus niger industrial wastes. | waste mycelia of aspergillus niger from a citric acid production plant are simply treated with boiling 30-40% naoh aqueous solutions for 4-6 hr to obtain the insoluble chitosan-glucan complex whose infrared, esr, and x-ray diffraction spectra are reported. a number of transition- and post-transition-metal ions are chelated and collected by chitosan-glucan with higher yields than by animal chitosan. immediate flocculation occurs upon mixing chitosan-glucan dispersions with alginate and polymolybd ... | 1980 | 7362863 |
| the effects of tunicamycin on protein glycosylation in mammalian and fungal systems. | | 1980 | 7371958 |
| significance of aspergillus species isolated from respiratory secretions in the diagnosis of invasive pulmonary aspergillosis. | to determine the significance of aspergillus species isolated from sputum or other respiratory secretions with respect to the diagnosis of invasive pulmonary aspergillosis, the clinical records and radiographs of all patients whose respiratory secretion cultures yielded an aspergillus species between 1972 and 1978 were reviewed. all known predispositions to invasive aspergillosis, e.g., presence of cancer or granulocytopenia, and therapy with corticosteroids, antibiotics, and cytotoxic drugs, we ... | 1980 | 7372799 |
| antifungal properties of n-alkanols, alpha, omega-n-alkanediols, and omega-chloro-alpha-alkanols. | fourteen n-alkanols (c1-c12, c14, and c16), 13 alpha,omega-n-alkanediols (c2-c12, c14, and c16), and 13 omega-chloro-alpha-alkanols (c2-c12, c14, and c16) were tested against aspergillus niger, trichoderma viride, and myrothecium verucaria in sabouraud dextrose agar at ph 4.0 and 5.6. toxicity to candida blbicans, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. the fungitoxicity of these alcohols was ... | 1980 | 7373528 |
| formation of nicotinamide ribose diphosphate ribose, a new metabolite of the nad pathway, by aspergillus niger. | | 1980 | 7374464 |
| isolation, purification and partial characterization of alkaline phosphatase from aspergillus niger. | | 1980 | 7380079 |
| isolation and characterization of a chlorogenic acid esterase from aspergillus niger. | the isolation and characterization of a specific chlorogenic acid esterase is described. the enzyme activity is measured by determination of the hydrolysis product caffeic acid. the enzyme had been concentrated by means of ultrafiltration and column-chromatography. the ph- and temperature optimum were 6.5 and 45 degrees c respectively. divalent cations were not required for the enzyme activity. as other esterases, this enzyme is inhibited by di-isopropyl-phosphorofluoridate. the km-value is 0.70 ... | 1980 | 7385941 |
| [resistance of microorganisms of upper layers of the atmosphere to ultraviolet radiation and a high vacuum]. | solar radiation and high vacuum are the main factors affecting the incidence of microorganisms in the stratosphere and mesophere. it has been established in experiments that the conidia of aspergillus niger are most resistant of uv irradiation. the conidia of penicillium, the spores of papulaspora anomala and circinella muscae, and the vegetative cells of micrococcus and mycobacterium are more susceptible to uv radiation. all of the isolated microorganisms have been shown to be resistant to high ... | 1980 | 7392991 |
| infection of a burn wound by aspergillus niger. | | 1980 | 7395812 |
| i and h activities of human red blood cells treated with an 1, 2-alpha-l-fucosidase from aspergillus niger. | after treatment with the 1, 2-alpha-l-fucosidase of aspergillus niger, the h antigen activity of red cells from normal h phenotypes is abolished and i activity is simultaneously increased. we suggest that this change could be related to the better accessibility to antibodies of the i erytrocyte receptors. | 1980 | 7406995 |
| pyridine nucleotide levels and ratios in aspergillus niger. | a reliable method for the extraction and assay of pyridine nucleotides (nad, nadh, nadp, and nadph) in filamentous fungi is presented. the method is applied to a study of the physiology of citric acid accumulation by aspergillus niger. the measurement of "catabolic reduction change" (nadh/(nad + nadh)) and "anabolic reduction charge" (nadph/(nadp + nadph)) as a means of characterizing the metabolic state is discussed. | 1980 | 7407716 |
| [effect of detergents on the hydroxylation of indolyl-3-acetic acid by an aspergillus niger culture]. | a possibility to increase the hydroxylating activity of aspergillus niger ibfm f-212 under the action of detergents was studied during transformation of indolyl-3-acetic acid (iaa). the following non-ionogenic surface-active compounds were mainly used: tweens, spans, polyethyleneglycol (peg-400). the effect of the detergents was studied at the stages of growth, transformation and preincubation. at the stage of growth, the best effect was produced by tween-80. at the stages of transformation and ... | 1980 | 7412617 |
| regulation of citrate synthase from the citric acid-accumulating fungus, aspergillus niger. | citrate synthase (citrate oxaloacetate-lyase (pro-3s-ch2coo leads to acetate-coa), ec 4.1.3.7) was purified 66-fold from cell-free extracts of a citric acid producing strain of aspergillus niger. the enzyme is labile at low ionic strength, but can effectively be stabilized by k+, oxaloacetate or glycerol. it has a molecular weight of 80 000 and an optimum ph of 8.5. the enzyme is activated by monovalent cations in dilute buffer solutions, and inhibited by mg2+ independent of the buffer molarity. ... | 1980 | 7417457 |
| an application of ellipsometry. assessment of polysaccharide and glycoprotein interaction with lectin at a liquid/solid interface. | lectins were specifically adsorbed from solution onto metallized glass slides coated with polysaccharide, glycopeptide and glycoprotein films. the degree of interaction was determined by measuring the thickness of the bound lectin layer with an ellipsometer after washing and drying the slide. the binding of concanavalin a (tetrameric) and succinyl concanavalin a (dimeric) to a yeast mannan film was studied as a function of lectin concentration, temperature, rinsing time and the extent of stirrin ... | 1980 | 7417529 |
| application of immobilized enzymes to clinical analyses : use of co-immobilized glucose oxidase and peroxidase in column form. | glucose oxidase from aspergillus niger and peroxidase from horseradish were simultaneously immobilized onto alkylamine glass beads which were then packed into a 1.5 x 20 mm column and integrated in the flow system of an autoanalyzer i. glucose in serum, up to 5.0 g/l, was continuously determined at a rate of 60 samples per hour. the co-immobilized enzyme column gave better sensitivity as compared with an enzyme column which contained a mixture of two kinds of individually immobilized enzymes. th ... | 1980 | 7417593 |
| the relationship between pleurotus ostreatus and aspergillus flavus and the production of aflatoxin. | the relationship between pleurotus ostreatus and aspergillus flavus in common mixed culture on various substrates was investiaged. it was found that p. ostreatus, similarly to some other higher fungi, can liquidate colonies of a. flavus. this fungus does not produce aflatoxin and chromatographically similar compounds. on straw, corn cobs, millet and wheat a. flavus produced aflatoxin after a 3-week cultivation. a subsequent cultivation of p. ostreatus led to detoxication of straw and corn cobs b ... | 1980 | 7419131 |
| rhizosphere fungi of five plants in egypt. | 75 species which belong to 25 genera were collected during this investigation. all of these fungi were previously isolated from egyptian soils and other sources. the total count and the number of genera and species in the rhizosphere soil of the 5 plants were regularly higher than in non-rhizosphere soil. the composition of the rhizosphere fungus flora was considerably influenced by the type and the age of the plant. the most prevalent fungi in the rhizosphere of the test plants after 45, 90, an ... | 1980 | 7424047 |
| biochemical studies on acetate non-utilizing (acu) mutants of aspergillus niger. | | 1980 | 7429599 |
| purification of an alpha-l-arabinofuranosidase from aspergillus niger by substrate affinity chromatography. | | 1980 | 7430310 |
| [effect of products of thermophilic methane fermentation on the conidial activation and citric acid biosynthesis by the fungus aspergillus niger]. | the effect of products of thermophilic methane fermentation (ptmf) and their hydrolyzates on the conidial germination and citric acid bioysnthesis by the fungus aspergillus niger when cultivated on the synthetic and molasses media was investigated. an addition of the above products increased the amount of germinated conidia on the synthetic medium by 1.2--2 times and on the molasses medium by 1.1--1.4 times. the citric acid yeild per unit surface area on the two media grew by 60--100% and 10--20 ... | 1980 | 7433429 |
| purification, properties, kinetics, and mechanism of beta-n-acetylglucosamidase from aspergillus niger. | beta-n-acetylglucosaminidase has been purified from an acetone extract of aspergillus niger. the protein has a mr = 149,000. it contains neither mn2+, zn2+, nor cysteine and exhibits no cation requirement for activity. isoelectric focusing separates two isozymes; the major isoenzyme has a pi = 4.4. both isozymes exhibit beta-n-acetylgalactosaminidase and beta-glucosidase, as well as glucosaminidase activity. the mechanism of action of this enzyme has been studied in detail using a variety of sub ... | 1980 | 7440573 |
| [isolation and characterization of an acetylester-hydrolase from aspergillus niger (author's transl)]. | the characteristic features of an acetic acid esters hydrolyzing enzyme (acetylesterase, ec 3.1.1.16) are described. the ph- and temperature optimum were 7.0 and 40 degrees c respectively. the stability of the enzyme regarding different ph- and temperature conditions was investigated. the molecular weight of the acetylesterase could be determined to 160 000. a small acetic ester hydrolyzing activity was found too with a molecular weight of about 25 000. the activity was not inhibited by addition ... | 1980 | 7445676 |
| [further characterization of a chlorogenic acid hydrolase from aspergillus niger (author's transl)]. | in addition to our previous paper [1] further characteristics of the chlorogenic acid hydrolase are described. polyacrylamide gel electrophoresis revealed only one band for the purified enzyme. sodium dodecyl-sulfate polyacrylamide gel electrophoresis showed a molecular weight of 60 000, demonstrating four subunits of the enzyme (total molecular weight 240 000). the enzyme is stable in a ph-range of 3.0--8.5 and up to a temperature of 55 degrees c. the temperature coefficient q10 is 1.5, the act ... | 1980 | 7445677 |
| the influence of ultrasonic waves, alone and in presence of certain salts on the microbial production of citric acid by aspergillus niger. | the influence of ultrasonic waves, alone and in presence of different (0.1, 0.5, and 1.0%) concentrations of nacl, kh2po4 and nh4cl on the microbial production of citric acid by aspergillus niger was studied. it was observed that in presence of sonic waves alone the yield of citric acid was generally better than that of control. besides, some morphological changes in the diameter of the conidiophore of the of the mould was also noted. the effect of sonics in presence of nacl was inconspicuous si ... | 1980 | 7456808 |
| influence of manganese on morphology and cell wall composition of aspergillus niger during citric acid fermentation. | morphology and cell wall composition of aspergillus niger were studied under conditions of manganese sufficient or deficient cultivation in an otherwise citric acid producing medium. omission of mn2+ (less than 10(-7) m) from the nutrient medium of aspergillus niger results in abnormal morphological development which is characterized by increased spore swelling, and squat, bulbeous hyphae. fractionation and analysis of manganese deficient cell walls revealed increased chitin and reduced beta-glu ... | 1980 | 7458536 |
| [obtaining pectinolytic mutants of aspergillus niger]. | | 1980 | 7467410 |
| bms-192548, a tetracyclic binding inhibitor of neuropeptide y receptors, from aspergillus niger wb2346. i. taxonomy, fermentation, isolation and biological activity. | during the screening of microbial fermentation extracts for their ability to inhibit the binding of 125i-peptid yy (pyy) to the neuropeptide y (npy) receptor using the scintillation proximity assay (spa), bms-192548 was isolated from the extract of aspergillus niger wb2346 by bioassay-guided fractionation. bms-192548 showed the inhibitory activity against 125i-pyy binding to sk-n-mc and sms-kan cells, which express npy1 and npy2 receptors, respectively, with ic50 values of 24 microm in y1 and 27 ... | 1995 | 7490206 |
| bms-192548, a tetracyclic binding inhibitor of neuropeptide y receptors, from aspergillus niger wb2346. ii. physico-chemical properties and structural characterization. | the structure of bms-192548, a tetracyclic binding inhibitor of neuropeptide y receptors, was established by spectroscopic methods. the compound has an unusual b-c-d ring beta-diketone moiety. | 1995 | 7490207 |
| [the distribution of sleep and wakefulness in human african trypanosomiasis]. | last century, patients with human african trypanosomiasis were described as sleepy by day and restless by night, and physicians referred to this condition as sleeping sickness. such a description could have evoked a disturbance of circadian rhythms. however, it is only in 1989 that the first 24-hour recording was performed by our team in niamey (niger) in a patient with sleeping sickness. the patient was a niger-born farm worker who had contracted the disease near gagnoa (côte d'ivoire). polysom ... | 1994 | 7496201 |
| monoclonal antibodies against a 97-kilodalton antigen from aspergillus flavus. | we prepared a panel of five monoclonal antibodies (mabs) directed against aspergillus flavus that all reacted against one 97-kda antigen by western blot (immunoblot). flow cytometry demonstrated that these antibodies bound (in increasing degrees) to all morphologic stages of a. flavus growth: conidia, swollen conidia, and hyphae. cross-reactivity among species was examined by enzyme-linked immunosorbent assay of fungal culture filtrates. four mabs reacted with 10 of 11 a. flavus isolates, and th ... | 1994 | 7496924 |
| a novel chemoenzymatic glycosylation strategy: application to lysozyme modification. | hen egg lysozyme has been non-specifically glycosylated using a novel two-step strategy. first, a number of sucrose molecules have been chemically bound to the protein surface lysines, then the glycosidic chains have been enzymically lengthened, using a glycosyltransferase. for this task, a fructosyltransferase and a levansucrase have been tested, the latter appearing as the most effective one. in all cases, reactions have been optimised and several degrees of modification have been obtained. fi ... | 1995 | 7498482 |
| changes in dehydrogenases activity, number and ultrastructure of mitochondria in aspergillus niger mycelium growing on molasses media. | effects of toxic molasses compounds and of the antifoamer (spumol bj) on dehydrogenases activity, on the number and ultrastructure of mitochondria in a. niger mycelium of two strains characterized by different tolerance to toxic agents, were observed. in spite of significantly higher dehydrogenases activity in the intolerant strain (r-16) mycelia developing both on productive molasses in the presence of the defoamer and on non-productive molasses are characterized by marked reduction in the acti ... | 1993 | 7504876 |
| nitrogen, carbon, and ph regulation of extracellular acidic proteases of aspergillus niger. | aspergillus niger secretes a number of enzymes, including proteases, into its culture fluid. the regulation of the two major acidic extracellular proteases, pepa and pepb, was investigated using northern analyses. our data suggest that the regulation of pepa and pepb expression occurs predominantly at the level of mrna content and that, while they are regulated in a similar manner, differences are also clear in their expression. both genes were found to be under complex regulatory control. the e ... | 1994 | 7532112 |
| glucoseoxidase from aspergillus niger in reverse micelles: ph and wo dependence. | the well known enzymatic method for the determination of glucose in aqueous solution with the system glucose oxidase/peroxidase/o-dianisidine was modified in a way that enables the quantitative determination of glucose in various reverse micellar systems. for instance in the 0.1 m sodium-bis-(2-ethylhexyl)-sulfosuccinate/n-octane system the useable glucose measuring range is between 10 and 70 micrograms glucose per 3 ml micellar solution. investigations concerning the influence of the ph-value a ... | 1994 | 7535322 |
| molds modulate the release of histamine and sulfidoleukotrienes from human basophils. | | 1995 | 7542088 |
| an in vitro procedure for studying enzymic dephosphorylation of phytate in maize-soyabean feeds for turkey poults. | an in vitro method was developed to predict inorganic p release from maize-soyabean poultry feeds containing supplemental phytase (ec 3.1.3.8), and to quantify the effect of acid phosphatase (ec 3.1.3.2), fungal protease (ec 3.4.23.6) and aspergillus niger cellulase (ec 3.2.1.4) on phytate dephosphorylation. pepsin (ec 3.4.23.1) and pancreatin digestion periods were preceded by a 30 min pre-incubation at ph 5.25 to simulate digestion in the crop of poultry. pancreatin digestion was carried out i ... | 1995 | 7547827 |
| characterization of conformational preferences in a partly folded protein by heteronuclear nmr spectroscopy: assignment and secondary structure analysis of hen egg-white lysozyme in trifluoroethanol. | 2d and 3d heteronuclear nmr methods have been used to characterize the structure of hen egg-white lysozyme in a partially folded state using uniformly 15n-labeled protein. this state is formed by the denaturation of the protein in 70% trifluoroethanol (tfe)/30% water (v/v) and is characterized by substantial helical secondary structure in the absence of extensive tertiary interactions. 15n-filtered 3d noesy and tocsy experiments have allowed the sequential assignment of resonances for all but 2 ... | 1995 | 7548086 |
| participation of a proton-translocating plasma membrane atpase, acid phosphatase and alkaline phosphatase in atp degradation by aspergillus niger extracts. | extracts of a. niger could catalyze sequential hydrolysis of the three phosphate moieties of the atp molecule optimally at ph 2 and at ph 8. at ph 2 the hydrolysis was effected by an atpase followed by acid phosphatase while at ph 8 alkaline phosphatase was the only involved enzyme. separation of these three phosphate-hydrolyzing enzymes was achieved by sephadex g-100 column chromatography. the a. niger atpase seems to have two unique features. first, it was easily solubilized in distilled water ... | 1995 | 7548149 |
| nucleotide sequence and expression of alpha-glucosidase-encoding gene (agda) from aspergillus oryzae. | we have isolated an alpha-glucosidase(agl)-encoding gene (agda) from aspergillus oryzae by heterologous hybridization using the corresponding aspergillus niger gene as a probe. southern hybridization analysis showed that the agda gene is on a 5.0-kb scai fragment and there is a single copy in the a. oryzae chromosome. comparison with the a. niger agda gene indicated that the agda gene contains three putative introns from 52 to 59 nucleotides long, and that it encodes 985 amino acid residues. the ... | 1995 | 7549103 |
| spectrophotometric assay for superoxide dismutase based on the nitroblue tetrazolium reduction by glucose-glucose oxidase. | a new spectrophotometric assay of superoxide dismutase (sod) is described. the assay is based on the sod-mediated inhibition in the rate of nitroblue tetrazolium reduction to the blue formazan at alkaline ph. the optimized assay of sod is performed in 50 mm glycine-naoh buffer, ph 9.5, at 25 degrees c. the sod concentration is determined from the v/v ratio of rates measured in the absence (v) or the presence (v) of sod. one unit of sod has been defined as the concentration that decrease the rate ... | 1995 | 7549963 |
| fungal growth within a silicone tissue expander: case report. | fungal contamination of tissue expanders has not previously been reported. there are, however, reports of fungi in association with inflatable breast prostheses. colonisation of a tissue expander with aspergillus niger resulting in mechanical obstruction of the device is described. the possible modes of inoculation and survival of the organism within the expander envelope were studied including an investigation of the permeability to gases of the silicone expander envelope. recommendations are m ... | 1995 | 7551517 |
| a novel strategy for the isolation of defined pyrg mutants and the development of a site-specific integration system for aspergillus awamori. | a homologous gene transfer system for aspergillus awamori for site-specific integration is described, based on two components. first, a defined a. awamori pyrg mutant strain constructed by a selection strategy for gene-replacement in fungi. second, a vector with a homologous pyrg selection marker containing a defined mutation at a site different from that of the mutations in the pyrg gene of the defined mutant strain. defined mutation in the a. awamori pyrg gene, isolated from a genomic library ... | 1995 | 7553938 |
| molecular cloning and sequencing of the gene encoding an extracellular aspartic proteinase from aspergillus fumigatus. | oligonucleotide primers based on conserved regions of the aspergillopepsins (ec 3.4.23.18) were used to pcr amplify a 650 bp segment of the gene encoding the extracellular aspartic proteinase (pep) from aspergillus fumigatus. the segment was used as a probe for isolating and sequencing the gene from a genomic library of the fungus. likewise the cdna was amplified by reverse pcr, cloned and sequenced. the pep gene was found to consist of four exons encoding for 395 aa. the pre-proenzyme deduced h ... | 1995 | 7557298 |
| the aspergillus niger (ficuum) apha gene encodes a ph 6.0-optimum acid phosphatase. | we have used the aspergillus niger (an) apha gene as a probe and cloned the a. ficuum (af) srrc 265 gene encoding an extracellular ph 6.0-optimum acid phosphatase (apase6) from a genomic library. the identity of the af apha gene was confirmed and its nucleotide (nt) sequence verified by comparing its deduced amino acid (aa) sequence to that of purified af apase6. a comparison of the nt sequences of the an and af genes suggested that errors were made in the previously reported an apha sequence. s ... | 1995 | 7557398 |
| cloning and characterization of three aspergillus niger promoters. | an aspergillus niger (an) genomic library was constructed using the promoter-trap vector, plx2a, which contains a hygromycin b (hy) phosphotransferase-encoding gene (hph) for selection of dna fragments with promoter activity. this library was transformed in escherichia coli and 80,000 colonies were obtained, 94% of which contained inserts. transformations of plasmid dna from the library into an resulted in 53 hy-resistant (hyr) colonies. southern blot analysis of 21 transformants confirmed the i ... | 1995 | 7557461 |
| molecular typing of aspergillus species. | in the last four years, several molecular typing methods have been applied to various aspergillus species, primarily a. fumigatus and a. niger. many 'clones' or dna types exist in the environment and in patients. the most discriminatory methods appear to be restriction fragment length polymorphism analysis with or without specific probes and random amplification of polymorphic dna analysis. insufficient work on interlaboratory reproducibility has been done. most patients are infected by single d ... | 1995 | 7560970 |
| studies on fungi associated with tomato fruits and effectiveness of some commercial fungicides against three pathogens. | among 39 fungal species and one variety belonging to 16 genera isolated from 116 healthy tomato fruits collected from markets in assiut, egypt, during 1994, aspergillus niger was found to be the most prevalent, being isolated from 84.6% of the samples. of 11 species recovered from 156 diseased tomatoes, alternaria alternata, rhizopus stolonifer and a. niger were the most common and isolated from 52.7%, 35.9% and 25.0% of the samples, respectively. experiments for comparison of the effects of med ... | 1995 | 7566057 |
| [survival of microscopic filamentous fungi under various temperature conditions]. | time required for devitalization of mucor fragillis, fusarium moniliforme, penicillium glabrum and aspergillus niger germs at temperatures of 22, 37, 60 degrees c was determined under laboratory conditions. all of the tested strains withstood the temperature 22 degrees c during the period of 42 days in sabouraud agar. at the temperature of 37 degrees c devitalization appeared in the following order: f. moniliforme 14 days, p. glabrum 21 days, m. fragillis 28 days, a. niger 35 days. temperature o ... | 1995 | 7571248 |
| cry j 2, a major allergen of japanese cedar pollen, shows polymethylgalacturonase activity. | we examined cry j 2, a major allergen of japanese cedar (cryptomeria japonica) pollen, for polygalacturonase enzyme activity, since a nucleotide sequence of cdna of cry j 2 showed a significant homology with that of tomato polygalacturonase. polygalacturonase is well known to depolymerize preferentially polygalacturonic acid (pga) by hydrolysis. however, cry j 2 did not act on pga, but was found to depolymerize pectin and methylesterified pga in a dose-dependent manner. the substrate specificity ... | 1995 | 7573841 |
| isolation and characterization of polygalacturonase genes (peca and pecb) from aspergillus flavus. | two genes, peca and pecb, encoding endopolyglacturonases were cloned from a highly aggressive strain of aspergillus flavus. the peca gene consisted of 1,228 bp encoding a protein of 363 amino acids with a predicted molecular mass of 37.6 kda, interrupted by two introns of 58 and 81 bp in length. accumulation of peca mrna in both pectin- or glucose-grown mycelia in the highly aggressive strain matched the activity profile of a pectinase previously identified as p2c. transformants of a weakly aggr ... | 1995 | 7574642 |
| efficacy of ozonated water against various food-related microorganisms. | the antimicrobial effects of ozonated water in a recirculating concurrent reactor were evaluated against four gram-positive and four gram-negative bacteria, two yeasts, and spores of aspergillus niger. more than 5 log units each of salmonella typhimurium and escherichia coli cells were killed instantaneously in ozonated water with or without addition of 20 ppm of soluble starch (ss). in ozonated water, death rates among the gram-negative bacteria--s. typhimurium, e. coli, pseudomonas aeruginosa, ... | 1995 | 7574656 |
| effect of polyols on heat inactivation of aspergillus niger van teighem inulinase. | the effect of polyols (ethylene glycol, glycerol, erythritol, xylitol and sorbitol) on partially purified inulinase from aspergillus niger van teighem mutant grown on kuth (saussurea lappa) root as source of inulin was determined. seventy per cent of inulinase activity was retained in the presence of 4 mol l-1 sorbitol at 75 degrees c. | 1995 | 7576522 |
| production and properties of three pectinolytic activities produced by aspergillus niger in submerged and solid-state fermentation. | three extracellular pectinases were produced by aspergillus niger ch4 by submerged and solid-state fermentation, and their physicochemical and kinetic properties were studied. the highest productivities of endo- and exo-pectinase and pectin lyase were obtained with solid-state fermentation. the kinetic and physicochemical properties of these enzymes were influenced by the type of culture method used. all activities were very different in terms of ph and temperature optima, stability at different ... | 1995 | 7576547 |
| cloning, sequence and expression of the gene coding for rhamnogalacturonase of aspergillus aculeatus; a novel pectinolytic enzyme. | rhamnogalacturonase was purified from culture filtrate of aspergillus aculeatus after growth in medium with sugar-beet pulp as carbon source. purified protein was used to raise antibodies in mice and with the antiserum obtained a gene coding for rhamnogalacturonase (rhga) was isolated from a lambda cdna expression library. the cloned rhga gene has an open-reading frame of 1320 base pairs encoding a protein of 440 amino acids with a predicted molecular mass of 45 962 da. the protein contains a po ... | 1995 | 7576553 |
| enzymatic synthesis of mannobioses and mannotrioses by reverse hydrolysis using alpha-mannosidase from aspergillus niger. | various manno-oligosaccharides including alpha-d-man-(1 --> 2)-d man and alpha-d-man-(1 --> 2)-alpha-d-man-(1 --> 2)-d-man were formed when a highly concentrated mannose solution was incubated in the presence of alpha-mannosidase from aspergillus niger. alpha-d-man-(1 --> 2)-d-man and alpha-d- man-(1 --> 2)-alpha-d-man-(1 --> 2)-d-man were isolated by activated carbon chromatography followed by high performance liquid chromatography using an amino-silica column. in addition to the above oligosac ... | 1995 | 7585696 |
| expression in aspergillus niger of the starch-binding domain of glucoamylase. comparison with the proteolytically produced starch-binding domain. | glucoamylase 1 from aspergillus niger is an economically important enzyme in many industrial processes. it hydrolyses granular starch and comprises two distinct domains, a catalytic and a starch-binding domain (sbd). we have transformed a. niger with an expression vector for the secretion of sbd for physico-chemical studies. this was achieved by introducing into the glucoamylase gene a short sequence encoding an endoproteolytic cleavage recognition site such that free sbd was secreted at yields ... | 1995 | 7588802 |
| 1h and 15n assignments and secondary structure of the starch-binding domain of glucoamylase from aspergillus niger. | 1h and 15n nmr resonance assignments of the granular starch-binding domain (sbd) of glucoamylase from aspergillus niger have been made by multi-dimensional homonuclear and heteronuclear nmr techniques. secondary structure analysis based on chemical shifts, 1h-1h noes, coupling constants and backbone amide exchange data indicates the presence of a well-defined beta-sheet structure. this consists of one parallel and five antiparallel pairs of beta-strands forming two beta-sheets. cis-trans isomeri ... | 1995 | 7588803 |
| efficacy of a recombinant-derived phytase in improving the bioavailability of phosphorus in corn-soybean meal diets for pigs. | three experiments involving 162 pigs were conducted to assess the efficacy of phytase (natuphos; basf, mount olive, nj) in low-p, corn-soybean meal-based diets. the phytase was produced by a recombinant aspergillus niger. the phytase supplement contained 5,000 phytase units (ptu)/g. in exp. 1 (66 pigs) and 2 (60 pigs), growing-finishing pigs were fed fortified corn-soybean meal diets formulated to be adequate (.50%), marginal (.425%), or inadequate (.35%) in p during the growing phase (23 to 60 ... | 1995 | 7592084 |
| phanerochaete chrysosporium glyoxal oxidase is encoded by two allelic variants: structure, genomic organization, and heterologous expression of glx1 and glx2. | a cdna clone (glx-2c) encoding glyoxal oxidase (glox) was isolated from a phanerochaete chrysosporium lambda gt11 library, and its nucleotide sequence was shown to be distinct from that of the previously described clone glx-1c (p. j. kersten and d. cullen, proc. natl. acad. sci. usa 90:7411-7413, 1993). genomic clones corresponding to both cdnas were also isolated and sequenced. overall nucleotide sequence identity was 98%, and the predicted proteins differed by a single residue: lys-308<==>thr- ... | 1995 | 7592374 |
| production and application of monoclonal antibodies specific to pyrroloquinoline quinone. | we produced five monoclonal antibodies (mabs 1, 2, 6, 7, and 9) that are specific to pyrroloquinoline quinone (pqq). pqq-conjugated hemocyanin was used for the immunization of mice and the hybridomas were selected using pqq-conjugated bsa in an enzyme-linked immunosorbent assay. mabs 2 and 9 were of the igg1 isotype. both could recognize free pqq, the former probably at the o-quinone and the latter at the opposite side of the molecule. they did not bind with trihydroxyphenylalanine, dihydroxyphe ... | 1995 | 7592546 |
| [otomycosis and topical application of thimerosal: study of 152 cases]. | to evaluate the effectiveness of the topical application of timerosal (merthilate tintura) in mycosis involving the external auditory canal. | 1995 | 7598973 |
| 2,3-dihydroxybenzoic acid decarboxylase from aspergillus niger. a novel decarboxylase. | 2,3-dihydroxybenzoic acid decarboxylase, the last enzyme in the fungal metabolism of indole to catechol, catalyzes the non-oxidative decarboxylation of 2,3-dihydroxybenzoic acid to catechol. unlike most other decarboxylases, this enzyme does not require a cofactor, underlining the importance of active-site residues in the reaction mechanism. earlier studies from this laboratory [kamath, a. v., appaji rao, n. & vaidyanathan, c. s. (1989) biochem. biophys. res. commun. 165, 20-26], have shown that ... | 1995 | 7601088 |
| efficacy of low-activity, microbial phytase in improving the bioavailability of phosphorus in corn-soybean meal diets for pigs. | two experiments involving 115 pigs were conducted to assess the efficacy of a microbial phytase (allzyme phytase; alltech, nicholasville, ky) produced by aspergillus niger in low-p, corn-soybean meal-based diets. the phytase supplement contained 50 phytase units/g and 1.43% p. in exp. 1, growing-finishing pigs were fed fortified corn-soybean meal diets formulated to be adequate (.50%) or inadequate (.30%) in p during the growing phase (38 to 57 kg bw) followed by adequate (.40%) or inadequate (. ... | 1995 | 7601778 |
| identification, characterization, and partial purification of glucoamylase from aspergillus niger (syn a. ficuum) nrrl 3135. | the crude extracellular extract of aspergillus niger (syn a. ficuum) nrrl 3135 contains glucoamylase (exo-1,4-alpha-d-glucanohydrolase, ec 3.2.1.2). the enzyme, a glycoprotein, was purified 7-fold by ion-exchange chromatography, chromatofocusing, and conconavalin a affinity chromatography. the molecular weight of the enzyme was estimated to be 90 kda by sds-page and gel permeation chromatography. the pi of the enzyme was 3.4. the temperature optimum of the enzyme was 60 degrees c and the ph opti ... | 1995 | 7603971 |
| false-positive antineutrophil cytoplasmic antibody in aspergillosis with oxalosis. | although highly specific for wegener's granulomatosis and other vasculitides, false-positive antineutrophil cytoplasmic antibody is occasionally encountered in other nonvasculitic conditions. we report a case of clinically suspected wegener's granulomatosis with a large pulmonary cavity, renal failure, and positive cytoplasmic antineutrophil cytoplasmic antibody reaction. the patient died before initiation of immunosuppressive therapy. postmortem examination revealed pulmonary aspergillosis due ... | 1995 | 7605176 |
| aspergillus peritonitis: therapy, survival, and return to peritoneal dialysis. | peritonitis caused by fungi of the species aspergillus is rare in peritoneal dialysis patients and is associated with high mortality. four of the six reported survivors have been unable to resume peritoneal dialysis. we report the successful treatment of aspergillus niger peritonitis with a combination of intravenous amphotericin b and peritoneal catheter removal in a patient on continuous ambulatory peritoneal dialysis (capd), and review previously reported cases of aspergillus peritonitis. our ... | 1995 | 7611273 |
| molecular cloning, expression and evaluation of phosphohydrolases for phytate-degrading activity. | four acid phosphatase (phosphomonoesterase e.c.3.1.3.2) genes were cloned by polymerase chain reaction (pcr). these were pho3, pho5 and pho11 from saccharomyces cerevisiae and the gene for a phosphate-respressible acid phosphatase from aspergillus niger. the individual genes were subcloned into an a. oryzae expression vector downstream from a starch-inducible alpha-amylase promoter and the resulting expression constructs were transformed into a mutant strain of a. oryzae, ao7. southern hybridiza ... | 1995 | 7612216 |
| effects of size of carbohydrate chain on protease digestion of aspergillus niger endo-beta-1,4-glucanase. | three different carbohydrate-depleted enzymes were prepared from an endo-beta-1,4-glucanase of aspergillus niger ifo31125 by treatment with endo-beta-n-acetylglucosaminidase or alpha-mannosidase. they were purified by concanavalin a-sepharose affinity and deae ion-exchange column chromatographies. the molecular sizes of these enzymes had been decreased from 40 kda containing 9.0% carbohydrate to 39, 38, and 37 kda with carbohydrate at 4.5, 1.3, and 0.8% (wt/wt), respectively. the native and thes ... | 1995 | 7612990 |
| zinc as a growth factor for aspergillus sp. and the antifungal effects of root canal sealants. | noninvasive aspergillosis of the maxillary sinus is frequently associated with extrusion of root canal cements into the sinus, and it has been suggested that zinc oxide-based materials might promote infection because zinc is a growth factor for aspergillus sp. to investigate this possibility pure zinc oxide-eugenol cement and five other cements were tested for antifungal activity against five strains of aspergillus fumigatus, one strain of a. niger, and one strain of a. flavus by zone inhibition ... | 1995 | 7614168 |
| detection of aspergillus species dna in bronchoalveolar lavage samples by competitive pcr. | a competitive pcr assay involving the use of bronchoalveolar lavage (bal) samples for the diagnosis of invasive pulmonary aspergillosis (ipa) was developed. for this purpose, a 1-kb mitochondrial dna fragment of aspergillus fumigatus was sequenced. the primers used allowed amplification of a. fumigatus, a. flavus, a. terreus, and a. niger dnas but not dnas of other fungi and yeasts. bal samples from 55 consecutively enrolled patients were tested. three samples were excluded because of failure of ... | 1995 | 7615723 |
| effect of corn and peanut cultivation on soil populations of aspergillus flavus and a. parasiticus in southwestern georgia. | the effect of corn and peanut cultivation on the proportion of aspergillus flavus to a. parasiticus in soil was examined. soil populations were monitored in three fields during three different years in southwestern georgia. each field was planted in both peanuts and corn, and soil was sampled within plots for each crop. a. flavus and a. parasiticus were present in similar proportions in plots from all fields at the beginning of the growing season. a. terreus, a. niger, and a. fumigatus were the ... | 1995 | 7618858 |
| sudden substrate dilution induces a higher rate of citric acid production by aspergillus niger. | on the basis of the present knowledge of aspergillus niger metabolism during citric acid fermentation, an idea on how to improve the process was formed. initially, a higher sucrose concentration was used for the germination of spores, which caused a higher intracellular level of the osmoregulator, glycerol, to be present. when citric acid started to be excreted into the medium, the substrate was suddenly diluted. optimization of this procedure resulted in a nearly tripled volumetric rate (grams ... | 1995 | 7618885 |
| thermodynamics of inhibitor binding to mutant forms of glucoamylase from aspergillus niger determined by isothermal titration calorimetry. | we have investigated the binding of mutant forms of glucoamylase from aspergillus niger to the inhibitors 1-deoxynojirimycin and acarbose. the mutants studied comprise a group of single amino acid replacements in conserved regions near the active site of the enzyme. for each mutant we have determined both the affinities for the two inhibitors and the thermodynamic state functions for binding using titration microcalorimetry. we find that acarbose binds to all the mutants with a wide range of bin ... | 1995 | 7640269 |
| mutational analysis of the roles in catalysis and substrate recognition of arginines 54 and 305, aspartic acid 309, and tryptophan 317 located at subsites 1 and 2 in glucoamylase from aspergillus niger. | the mutants arg54-->leu, arg54-->lys, arg305-->lys, asp309-->glu, and trp317-->phe, located at subsites 1 and 2 in glucoamylase from aspergillus niger, provide insight into the importance of specific hydrogen bonds and hydrophobic interactions in substrate recognition, catalytic mechanism, and stability. as suggested from the crystal structure of a closely related glucoamylase [aleshin, a. e., firsov, l. m., & honzatko, r. b. (1994) j. biol. chem. 269, 15631-15639], arg54 in subsite 1 hydrogen b ... | 1995 | 7640270 |
| effects of leader sequences upon the heterologous expression of restrictocin in aspergillus nidulans and aspergillus niger. | the effects of altered leader sequences on the secretion and localization of restrictiocin expression in aspergillus nidulans and aspergillus niger were investigated. the region encoding the leader sequence of the aspergillus restrictus restrictocin (res) gene was altered and variants were expressed under the glucoamylase (glaa) promoter in a. nidulans and a. niger. the entire restrictocin leader sequence was replaced by the glaa leader sequence in one variant. in another, the signal sequence of ... | 1995 | 7641142 |
| cloning and characterization of the nadph cytochrome p450 oxidoreductase gene from the filamentous fungus aspergillus niger. | in this paper, we describe the cloning and molecular characterization of the aspergillus niger cytochrome p450 reductase (cpr) gene, cpra. attempts to clone the cpra gene by heterologous hybridization techniques were unsuccessful. using the polymerase chain reaction (pcr) with degenerate primers based on conserved regions found in cpr genes from other organisms, we were able to isolate a fragment that contained part of the gene. with the aid of this fragment, a genomic fragment containing the en ... | 1995 | 7646819 |
| 1h- and 13c-nmr characterization of the digalactosylmannopentaose liberated from legume seed galactomannan by beta-mannanase action. | incubation of locust bean gum with an aspergillus niger beta-d-mannanase released a wide variety of galactomannan oligomers. a single heptasaccharide, digalactosylmannopentaose, was obtained from fractionation of the mixture by size exclusion chromatography. the purity and chemical composition of the sample was demonstrated using mass spectrometry, high performance anion-exchange chromatography and monosaccharide composition analysis. the primary structure of this heptasaccharide was unambiguous ... | 1995 | 7648582 |
| glycoprotein biosynthesis in saccharomyces cerevisiae: ngd29, an n-glycosylation mutant allelic to och1 having a defect in the initiation of outer chain formation. | outer chain glycosylation in saccharomyces cerevisiae leads to heterogeneous and immunogenic asparagine-linked saccharide chains containing more than 50 mannose residues on secreted glycoproteins. using a [3h]mannose suicide selection procedure a collection of n-glycosylation defective mutants (designated ngd) was isolated. one mutant, ngd29, was found to have a defect in the initiation of the outer chain and displayed a temperature growth sensitivity at 37 degrees c allowing the isolation of th ... | 1995 | 7649302 |
| comparative analysis of catalases: spectral evidence against heme-bound water for the solution enzymes. | a recent x-ray structural analysis of m. luteus catalase indicates heme-bound h2o trans to the proximal tyrosinate ligand, a finding in contrast to previous x-ray data reporting a 5-coordinate heme for bovine liver catalase. the presence of heme-bound h2o, requiring displacement prior to substrate-binding, is likely to be catalytically significant for catalases. we have used magnetic circular dichroism (mcd) spectroscopy, a highly accurate method for assignment of heme spin- and coordination-sta ... | 1995 | 7649312 |
| machine operator's lung. a hypersensitivity pneumonitis disorder associated with exposure to metalworking fluid aerosols. | six auto parts manufacturing workers were referred for evaluation of a 6-week history of work-related dyspnea, cough, and fatigue. two workers also reported fever and weight loss. all six worked in a machining area where a waterbased metalworking fluid was used and recirculated under high pressure, thereby creating an aerosol. chest radiographs revealed pulmonary interstitial infiltrates in four workers. lung function tests showed that four workers had decreased diffusing capacity. after removal ... | 1995 | 7656609 |
| [isolation of several species of the genus aspergillus from soil of intrahospital ornamental plants]. | the earth of ornamental plants is one of the main reservoirs of aspergillus type of fungi in hospital areas. we studied 174 ornamental interior plants from a hospital at santiago. samples were obtained from the soil surface and sowed in sabouraud-glucose agar, adding streptomycin and g-penicillin. after 72 h of culture, at least one strain of aspergillus was isolated from 140 samples (80.5%). the most frequently isolated strain was a fumigatus (129 samples), followed by a niger (75 samples). a f ... | 1994 | 7659910 |
| the primary structure of carboxypeptidase s3 from penicillium janthinellum ibt 3991. | the complete amino acid sequence of penicillopeptidase s3, a serine carboxypeptidase isolated from penicillium janthinellum ibt 3991, has been determined. the enzyme consists of 481 amino acids arranged in a single polypeptide chain. six glycosylation sites were established in positions 41, 218, 256, 326, 384 and 392. the molecule contains six cysteinyl residues among which disulfide bridges was established between cys-71-cys-333 and cys-233-cys-289. carboxypeptidase s3 is homologous to carboxyp ... | 1995 | 7664873 |
| phylogenetic relationships of five species of aspergillus and related taxa as deduced by comparison of sequences of small subunit ribosomal rna. | the nucleotide sequences of the genes encoding the 18s rrna of aspergillus flavus, a. nidulans, a. terreus and a. niger were elucidated and aligned to the sequences of a. fumigatus. in addition, the 18s rrna sequences of the v4-v9 region of morphologically similar filamentous fungi, e.g. penicillium chrysogenum, p. marneffei and paecilomyces variotii, were elucidated. phylogenetic analysis and comparison showed a very close intergeneric relationship of the genus aspergillus to species of the gen ... | 1995 | 7666299 |