| naphtho-gamma-pyrone production by aspergillus niger isolated from stored cottonseed. | aspergillus niger was found to be the predominant fungal contaminant of stored cottonseed. seven strains were isolated and grown on rice. the hexane-insoluble material from methylene chloride extracts of 2-week-old cultures contained components toxic to mice. based on high-pressure thin-layer and liquid chromatographic analyses, the major components in the mixture were eight different naphtho-gamma-pyrones. of these, the hydrated dimeric naphthopyrones aurasperones b and c occurred in higher yie ... | 1984 | 6548104 |
| mechanisms of inhibition by mevinolin (mk 803) of microsome-bound radish and of partially purified yeast hmg-coa reductase (ec.1.1.1.34). | 1) in kinetic studies, mevinolin proved to be a highly specific inhibitor of partially purified yeast hmg-coa reductase (ki = 3.5 nm towards hmg-coa) and of microsomal hmg-coa reductase from etiolated radish seedlings (ki = 2.2 nm). at low concentrations of nadph, the inhibitor counteracts the sigmoidal response of plant hmg-coa reductase activity towards the cosubstrate. at higher concentrations of nadph, the inhibition pattern is of non-competitive type. 2) our results are extensively compared ... | 1983 | 6553442 |
| effect of enzyme-matrix composition on potentiometric response to glucose using glucose oxidase immobilized on platinum. | glucose oxidase (beta-d-glucose:oxygen 1-oxidoreductase, ec 1.1.3.4) was immobilized in a crosslinked matrix of bovine serum albumin, catalase, glucose oxidase and glutaraldehyde on platinum foil. when placed in glucose solution, this enzyme-electrode elicited a potentiometric response that varied with the changes in glucose concentration. the immobilized glucose oxidase was present at 7.4-10.1 micrograms enzyme protein/ml of matrix, as determined with 125i-labelled enzyme. the coupled enzyme ac ... | 1983 | 6615849 |
| [qsar and aromatic series: proposals for steric parameters]. | a steric parameter, sigma d (the perimeter of the efficacious section of a molecule in the same plane as the aromatic ring) is proposed. a comparison between sigma d, rm and 1xv in six different series of aromatic molecules was made. in most cases the new parameter is preferable. | 1983 | 6617847 |
| minimal inhibitory concentrations of lucknomycin, a new polyenic derivative, for candida and aspergillus spp. | the minimal inhibitory concentrations (mics) of lucknomycin, a new polyenic derivative, were determined for 101 clinical isolates of candida, 38 clinical or environmental strains of aspergillus fumigatus, and 30 isolates of a. niger. the most susceptible species were candida albicans and candida tropicalis (mean mic, 0.4 micrograms/ml). aspergillus spp. were less susceptible, with mean mics of 0.60 micrograms/ml for aspergillus niger and 9.2 micrograms/ml for aspergillus fumigatus. | 1983 | 6625552 |
| inhibition of fungal nadp+-isocitrate dehydrogenase by citric acid. | the variations observed during earlier studies in the activity of nadp+-isocitrate dehydrogenase (ec. 1.1.1.42) in a strain of aspergillus niger were found to be related to the extent of washing of mycelium. as a result the mycelium washed four times with phosphate buffer (0.05 m, ph 7.5), the enzyme activity present in 4 and 8 days old fungal mycelia increased five- and two-fold, respectively. in vivo studies showed a complete loss of enzyme activity in mycelia resuspended in hcl-kcl buffer (0. ... | 1983 | 6636842 |
| studies on the antigenic composition of aqueous cotton dust extracts. | an immune response to cotton dust components has been proposed as a possible mechanism of byssinosis. to evaluate this possibility, specific precipitating antibodies against aqueous cotton dust extracts (acde) were raised in rabbits, and a reference pattern for crossed immunoelectrophoresis (cie) was established using this extract. a significant portion of the immune response was directed against alternaria tenuis, aspergillus niger, and fusarium solani, all documented contaminants of cotton dus ... | 1983 | 6642705 |
| cyclosporin a inhibits coccidioides immitis in vitro and in vivo. | balb/c mice infected intraperitoneally with coccidioides immitis were treated with cyclosporin (cya) subcutaneously. cya prevented infection when treatment was started at day zero. when treatment was delayed until day 6 after infection, the mice that received either 75 or 25 mg/kg per day survived, but those treated with 7.5 mg/kg per day had the same mortality rate as controls. the higher doses of cya prevented dissemination of the fungus from the peritoneum to the lung but did not eliminate th ... | 1983 | 6660859 |
| biological activity of 4-hydroxy-5-formylbenzoic acid derivatives. | a group of thirty 4-hydroxy-5-formylbenzoic acid derivatives of general formula: (formula; see text) where (formula; see text) have been prepared and characterized in an attempt to make available for testing a representative selection of hitherto undescribed hydroxyformylbenzoic acid derivatives. the products, which are listed in table i, with pertinent data, have been obtained in satisfactory yield and in a good state of purity. the prepared compounds have been tested for "in vitro" activity ag ... | 1983 | 6667309 |
| production of oxalic acid by some fungi infected tubers. | oxalic acid (as oxalate) was detected in four tubers commonly used for food in nigeria-dioscorea rotundata (white yam), solanum tuberosum (irish potato), ipomoea batatas (sweet potato), and manihot esculenta (cassava). whereas healthy i. batata had the highest oxalic acid content, healthy m. esculenta contained the lowest. when all tubers were artifically inoculated with four fungi-penicillium oxalicum curie and thom, aspergillus niger van tiegh, a. flavus and a. tamarii kita, there was an incre ... | 1983 | 6670294 |
| selective isolation of endo-d-galacturonanase of aspergillus niger based on interaction with tri(d-galactosiduronic acid) covalently bound to poly(hydroxyalkyl methacrylate). | a selective affinity-adsorbent for the extracellular endo-d-galacturonanase (e.c. 3.2.1.15) of aspergillus niger was prepared by covalent coupling of tri(d-galactosiduronic acid) to separon, a poly(hydroxyalkyl methacrylate) gel. complexing of the enzyme with the adsorbent is ph dependent; maximal interaction occurs at the optimum ph for enzyme activity. the enzyme was quantitatively displaced from the adsorbent either by changing the ph or by bioelution with soluble tri(d-galactosiduronic acid) ... | 1983 | 6671193 |
| quantitative structure activity considerations on the spore-inhibiting activity of esters of 1,3-dihydroxybutane. | esters of 1,3-dihydroxybutane inhibit the growth of the asexual spores of aspergillus niger. this activity is non-linearly related to lipophilic and steric parameters, e.g. partition coefficient, molecular connectivity and van der waal's volumes. the gauche parabolic shape of the activity curve allows a transformation in a simple, bilinear mathematical model. branched esters possess higher activity than the linear ones. the most potent molecule is the 2-ethylbutanoyl-1,3-dihydroxy-butane. | 1983 | 6672488 |
| [neonatal ulcero-necrotizing enterocolitis and aspergillosis]. | a preterm infant of 33 weeks of gestation and very small birthweight 1040 g developed on the third day of life a necrotizing enterocolitis with spontaneous perforation. peritoneal fluid and small bowel meconium was cultured and revealed aspergillus niger. the literature is reviewed with seven cases of aspergillosis in newborn period. the infant is dead on the tenth day of life from periventricular haemorrhage. necropsy was performed and revealed no secondary aspergillosis localisation. | 1983 | 6678409 |
| a new polyene with antimycotic activity produced by a streptomycete from the rhizosphere of an indoor plant. | from the rhizosphere of a potted sanseviera trifasciata, a streptomyces griseus strain with a broad antifungal activity was isolated; in the soil of this plant, a sole hyphomycete, aspergillus niger, was found in pure culture at 37 degrees c. this streptomycete produced a polyene, belonging to the pentaene group. it was found to inhibit, in vitro, the growth of all the 123 strains tested; the strains belonging to the genera aspergillus, cryptococcus, candida, trichophyton, epidermophyton, micros ... | 1983 | 6681974 |
| localized oxalosis associated with simultaneous aspergillus and mucor infection in diabetic foot gangrene. | a case of simultaneous aspergillosis and mucormycosis complicating diabetic foot gangrene is reported. the recognition of this combined opportunistic fungal infection was brought about by an initial finding of birefringent calcium oxalate crystals associated with a background of acute inflammatory exudate, which is considered a strong clue to the diagnosis of infection caused by aspergillus niger. | 1984 | 6693115 |
| aspergillus niger endocarditis complicating aortic tissue valve replacement. | | 1984 | 6695356 |
| pulmonary oxalosis caused by aspergillus niger. | a case report involving a pleuropulmonary mixed bacterial infection in association with aspergillus niger is described. the patient responded to a combination of antibiotics, aerosolized and intravenously administered amphotericin b, and surgery. aspergillus niger appeared to be a secondary invader and caused lung damage by the production of oxalic acid. | 1984 | 6703507 |
| [biological activity of 4-hydroxy-5-formylbenzoic acid derivatives. ii. esters and schiff bases with antimicrobial activity]. | a number of hitherto undescribed 4-hydroxy-5-formylbenzoic acid derivatives (a), have been prepared and characterized. (formula; see text) esters (x = ch3) and schiff's bases (z = n-aryl) were prepared by conventional methods and were obtained in satisfactory yield and in a good state of purity. the prepared compounds have been tested for "in vitro" activity against gram+ bacteria (s.epidermidis, b.subtilis, b.anthracis, m.paratuberculosis), gram- bacteria (p.aeruginosa, s.typhi murium, e.coli b ... | 1984 | 6712814 |
| pyruvate kinase from aspergillus niger: a regulatory enzyme in glycolysis? | pyruvate kinase from the filamentous, citric acid producing fungus aspergillus niger was purified about 100-fold by ammonium sulfate precipitation, deae-cellulose chromatography, and gel filtration. the addition of fructose-1,6-diphosphate was necessary to prevent loss of activity during purification. the enzyme purified in the presence of fructose-1,6-diphosphate (fdp) exhibits hyperbolic kinetics with respect to phosphoenolpyruvate (pep) and adp. monovalent cations activated the enzyme (k+, nh ... | 1984 | 6713301 |
| antimicrobial effects of esters and amides of 3-(5-nitro-2-furyl)acrylic acid. | the effect of 18 newly synthesized esters and amides of 3-(5-nitro-2-furyl)acrylic acid on bacteria (escherichia coli, staphylococcus aureus), yeasts (saccharomyces cerevisiae, candida albicans), molds (aspergillus niger, penicillium cyclopium, rhizopus oryzae) and algae (chlorella pyrenoidosa, euglena gracilis, scenedesmus obliquus) was investigated. the mic values revealed antimycotic, antialgal and antibacterial activity of the studied derivatives. the antimycotic activity was found to decrea ... | 1984 | 6714854 |
| in vitro activities of two new antifungal azoles. | the antifungal activities of equimolar quantities of three azole compounds, bay n 7133 [1-(4-chlorophenoxy)-3,3-dimethyl-2-(1,2,4-triazole-1-yl)methylbutan-2-o1], bay 1 19139 [1-(4-chlorophenoxy)-1-(1-imidazolyl)-3,3-dimethyl-2-butanol hydrochloride], and ketoconazole, were compared by testing the susceptibility in vitro of 10 clinical isolates each of candida albicans, candida parapsilosis, torulopsis glabrata, cryptococcus neoformans, aspergillus fumigatus, aspergillus niger, aspergillus flavu ... | 1984 | 6721465 |
| morphological anomalies induced by congo red in aspergillus niger. | aspergillus niger germ tubes were exposed for 6 h to 0.15 mg/ml of congo red, a stain which prevents chitin microfibril assembly. the most evident alterations, detected under ultraviolet light and by transmission and scanning electron microscopy, concerned the hyphal tips which burst or, most frequently, expanded into bulges. in the latter structures, new hyphal tips appeared which, after giving rise to more or less developed hyphae, were themselves converted into new bulges. therefore, segments ... | 1984 | 6721628 |
| composition of the fungal flora of four cereal grains in saudi arabia. | using the grain-plate method and on glucose- czapek 's agar at 28 degrees c, fifty-eight species belonging to 26 genera were collected from barley (42 species and 19 genera), maize (29 species and 16 genera), sorghum (32 species and 17 genera) and wheat grains (42 species and 18 genera). the most frequent genera were aspergillus, penicillium, rhizopus, fusarium, and mucor followed by alternaria, drechslera , and curvularia. from the preceding genera aspergillus flavus, a. niger, penicillium nota ... | 1984 | 6727980 |
| [aspergilli and chronic lung diseases]. | in this study, mycological examinations of morning sputum of patients with chronic bronchitis and bronchial asthma has been performed. specimens were also examined from patients with lobar pneumonia as a control. a fumigatus and a. niger has been isolated from patients chronic bronchitis in frequency of 4.5% and 0.8% respectively. these 2 fungi has also been isolated from patients with bronchial asthma in frequency of 24.5% (a. fumigatus) and 2.4% (a. niger). the frequency of isolation of a. fum ... | 1982 | 6755197 |
| laser microbeam studies on fungal tissues. | | 1982 | 6757676 |
| studies on air-borne fungi at qena. iii. thermophilic fungi. | | 1982 | 6759940 |
| [microbiological test for the determination of the risk of spoilage of solid drug forms by the growth of molds]. | | 1982 | 6760621 |
| high-yield method for immobilization of enzymes. | two types of polyethylenimine-coated glass microbeads (13-44 micrometer) were synthesized and used for the immobilization of glucose oxidase from aspergillus niger and catalase from a. niger and beef liver. the two types of beads were distinguishable by differences in their surface topography. immobilizations were performed by adsorption followed by treatment with glutaraldehyde. the immobilized-enzyme activities per unit support of all of the enzymes tested were compared with and found to be su ... | 1980 | 6766330 |
| interaction between aspergilli and streptomycetes in the soil of potted indoor plants: a preliminary report (contribution to the epidemiology of human aspergillosis). | the soil of potted ornamental plants as a reservoir for aspergillus species pathogenic for man is of epidemiological and ecological interest. isolation of a. niger as the sole hyphomycete from the soil of potted african violets (saintpaulia ionantha, gesneriaceae), prompted us to look for a. inger on the surface of the roots of this plant. small pieces of the roots were inoculated in the nutrient-free agar-gel with and without antibiotics. on the antibiotic-free gel, a dense growth of streptomyc ... | 1980 | 6769046 |
| [effect of metabolites of aspergillus genus fungi on water absorption in the large intestine of guinea pigs]. | | 1980 | 6769241 |
| air-borne fungi in the air of barcelona (spain). iii. the genus aspergillus link. | during a survey on the presence of species of the genus aspergillus in the air of the city of barcelona (spain), the following species were identified: aspergillus flavus link, a. niger van tieghem, a. fumigatus fresenius, a. clavatus desmazières, a. terreus thom, a. chevalieri (mang.) thom et church, a. niveus bloch, emend. thom et church, a. ochraceus wilhelm, a. versicolor (vuillemin) tiraboschi, and a. amstelodami (mang.) church et thom. | 1980 | 6770271 |
| biosynthesis of d-alanyl-lipoteichoic acid: characterization of ester-linked d-alanine in the in vitro-synthesized product. | d-alanyl-lipoteichoic acid (d-alanyl-lta) contains d-alanine ester residues which control the ability of this polyer to chelate mg(2+). in lactobacillus casei a two-step in vitro reaction sequence catalyzed by the d-alanine-activating enzyme and d-alanine:membrane acceptor ligase incorporates d-alanine into membrane acceptor. in this paper we provide additional evidence that the in vitro system catalyzes the covalent incorporation of d-[(14)c]alanine into membrane acceptor which is the poly([(3) ... | 1980 | 6772629 |
| structural information from molecular connectivity 4 chi pc index. | the molecular connectivity 4 chi pc index was examined for its ability to describe uniquely molecules containing substituted benzene rings. the subgraphs comprising this index were shown to encode information about the number, placement, and type of ring substituents. several examples illustrate the ability of the index to describe structure-influencing properties. | 1980 | 6774081 |
| glycoenzymes: an unusual type of glycoprotein structure for a glucoamylase. | glucoamylase, (1 leads to 4)(1 leads to 6)-alpha-d-glucan glucohydrolase (ec 3.2.1.3), hydrolyzes starch and glycogen completely to d-glucose and is used industrially in the manufacture of d-glucose from starch. the enzyme is elaborated by many types of fungi and occurs in two isoenzymic forms (glucoamylase i and glucoamylase ii) in extracts from certain fungi. the isoenzymes from aspergillus niger are glycoenzymes containing d-mannose, d-glucose, and d-galactose as integral structural component ... | 1980 | 6774807 |
| accumulation of 2-oxo acids in mutants of aspergillus niger requiring lysine. | mutants of aspergillus niger 194a and 178 requiring lysine differ from the original prototrophic strain k10 and from each other on the course of accumulation of organic acids. in both mutants less citric acid accumulates during the first phase of cultivation but considerably more 2-oxoglutarate and 2-oxoadipate accumulate than in the original strain. whereas in the 194a mutant this state remains unchanged also during the second phase of cultivation, in the 178 mutant oxo acids are degraded and c ... | 1980 | 6774936 |
| characterization of antigens from aspergillus fumigatus. v. reactivity in immunodiffusion tests with serums from patients with aspergillosis caused by aspergillus flavus, a. niger, and a fumigatus. | antigen-rich mycelial extract (me) and fractions of aspergillus fumigatus were compared with culture filtrates of a. fumigatus. a. flavus, and a. niger for their abilities to detect antibody in serums from patients with confirmed aspergillosis caused by these fungi. in double immunodiffusion tests, homologous antigen preparations were more effective than heterologous preparations in detecting precipitins. mycelial extract of a. fumigatus was more effective than a. fumigatus culture filtrate in ... | 1980 | 6776857 |
| ureide interactions in thiourea toxicity in aspergillus niger. | | 1980 | 6778816 |
| [effect of progressive chemical modification on the activity and thermal stability of soluble and immobilized glucoamylase]. | the chemical modification of glucoamylase from aspergillus niger with acryloylchloride has been studied. it was shown that the first 60--70% of the total amount of available amino groups of the enzyme readily interact with the reagent: the activity and thermal stability of the resulting soluble acryloylglucoamylase are only slightly less than those of the native enzyme. the modification of the remaining 30% of the total amount of amino groups is less intensive and results in a sufficient loss of ... | 1980 | 6783128 |
| biosynthesis of d-alanyl-lipoteichoic acid in lactobacillus casei: d-alanyl-lipophilic compounds as intermediates. | d-alanyl-lipoteichoic acid (d-alanyl-lta) from lactobacillus casei contains a poly(glycerol phosphate) moiety that is selectively acylated with d-alanine ester residues. to characterize further the mechanism of d-alanine substitution, intermediates were sought that participate in the assembly of this lta. from the incorporation system utilizing either toluene-treated cells or a combination of membrane fragments and supernatant fraction, a series of membrane-associated d-[14c]alanyl-lipophilic co ... | 1981 | 6783617 |
| induction, isolation, and characterization of aspergillus niger mutant strains producing elevated levels of beta-galactosidase. | an aspergillus niger mutant strain, vtt-d-80144, with an improvement of three- to fourfold in the production of extracellular beta-galactosidase was isolated after mutagenesis. the production of beta-galactosidase by this mutant was unaffected by fermentor size, and the enzyme was also suitable for immobilization. | 1981 | 6784672 |
| uptake of lysosomal enzymes by human fibroblasts: lack of uptake of fungal or plant glycosidases in comparison with a mammalian enzyme. | alpha-galactosidase from human placenta was actively taken up by cultured fibroblasts from patients with fabry's disease whereas the enzyme from the fig was not absorbed. alpha-glucosidase from two microbiological sources. a. fumigatus and a. niger, was not taken up by cultured fibroblasts from patients with pompe's disease. | 1980 | 6787335 |
| fungal pneumonia (part 4): invasive pulmonary aspergillosis. | | 1981 | 6788456 |
| [effect of dimilin on aspergillus niger growth and the formation of chitin]. | | 1981 | 6791709 |
| determining the kinetic behaviour for the secretion of milk toxin as related to dosage level of aflatoxin b1. | 39 species which belong to 14 genera were isolated from 110 milk samples collected from different dairy sources at assiut city. the most frequent fungi were a. niger, a flavus and clasdosporium cladosporioides, followed by curvularia spicifera and penicillium corylophilum. the remaining species were of rare occurrence. the fluorescence-method for detecting aflatoxin-producing strains demonstrated the presence of two isolates namely a. flavus possesses this property. one of these toxic isolates w ... | 1981 | 6794233 |
| thermal stabilization of glucose oxidase and glucoamylase by physical entrapment. | physical entrapment was used as an approach to achieve thermal stabilization of enzymes. the t 1/2 values for the thermoinactivation of glucose oxidase and glucoamylase were increased several-fold by their entrapment in polyacrylamide gels. in polyacrylate gels the individual enzymes behaved differently, probably owing to microenvironmental effects arising by the polyelectrolyte nature of the carrier. | 1981 | 6796045 |
| [enzymatic conversion of polymers. nature of apparent product inhibition in the course of enzymatic degradation of polymer substrates]. | the general regularities for the kinetics of enzymatic conversion of polymers were studied. it was proposed that the formal kinetic analysis of progress kinetic curves for enzymatic degradation of polymers inevitably results in the apparent effects of product inhibition, even in the case when the products are not virtually bound to the enzyme and there is no real product inhibition at all. an experimental verification of this hypothesis was performed, using maltodextrins hydrolysis by aspergillu ... | 1981 | 6796131 |
| estimation of lactose hydrolysis by freezing point measurements in milk and whey substrates treated with lactases from various microorganisms. | beta-galactosidase concentrates obtained from several microorganisms were used to hydrolyze skim milk, low fat (2%) milk, sweet whey, acid whey, acid whey permeate, and acid whey concentrate. among acid substrates, the freezing point depression for each 1% lactose hydrolyzed was the greatest with the lactase from aspergillus niger (0.0501 degrees h); among neutral substrates, the depression was greater in sweet whey (0.0495 degree h) and lesser in low fat milk (0.0445 degrees h). all data were s ... | 1981 | 6796559 |
| natural-abundance 13c-n.m.r. spectra of some model compounds for uncommon glycopeptide linkages in glycoproteins. | | 1981 | 6797730 |
| serodiagnostic value of culture filtrate antigens from aspergilli with septate phialides. | culture filtrate antigens from 6 typical isolates of aspergillus fumigatus, 6 isolates with septate phialides, and isolates of a. fumigatus var. ellipticus, a. fishceri, a. flavus and a. niger were studied in tests with 2,100 samples of serum originating from 1,452 patients suffering from miscellaneous diseases, including 60 cases of aspergillosis, 850 samples of serum from apparently healthy blood donors and 522 sera referred to us as positive for c-reactive protein. the culture filtrate antige ... | 1982 | 6801788 |
| action of glucoamylase from aspergillus niger on phosphorylated substrate. | glucoamylase (1,4-alpha-d-glucan glucohydrolase, ec 3.2.1.3) from aspergillus niger was purified to be free from alpha-amylase and phosphatase (glucose 6-phosphate as substrate). the phosphatase was well separated from the glucoamylase by phosphocellulose ion-exchange chromatography. the glucoamylase action was prevented by the esterified phosphate groups of the substrate. thus, the extensive action of the glucoamylase on potato starch exposed the 6-posphorylglucosyl residue of the starch at the ... | 1982 | 6803838 |
| mycobacterial antibodies after tuberculin testing, bcg- vaccination, bcg-immunotherapy and against cross-reacting antigens in a solid-phase radioimmunoassay. | a solid-phase radioimmunoassay (spria) was used to investigate several factors influencing false positive and negative results in serodiagnostic test for tuberculosis, unheated cytoplasmic fractions of mycobacteria, and other microorganisms were bound to polystyrol and antibody binding of sera from tuberculous, non tuberculous hospitalized patients and healthy adults was tested by the spria-method. the level of antibody concentration in the spria reflects roughly cross-reactivity between the ant ... | 1982 | 6805156 |
| structural similarities between a membrane-like surface layer on the cell wall of some microbial cells and the lamellar layer of vesicles formed by short-chain phosphonic acids in water. a freeze-fracture study. | a thin layer on the cell wall of some microbial cells, among them such of medical importance (aspergillus niger, trichophyton sp.), is characterized by a mosaic-like rodlet pattern in freeze-fracture electron microscopy. a very similar texture could be found in freeze-fractured bilayer vesicles of n-octan phosphonic acid in presence of 50% glycerol. the formation of this structure in phosphonic acid vesicles is connected with the transformation of the bilayer into the solid crystalline state by ... | 1982 | 6807710 |
| hydrophobic-ionic chromatography: its application to microbial glucose oxidase, hyaluronidase, cholesterol oxidase, and cholesterol esterase. | glucose oxidase from aspergillus niger, hyaluronidase from streptomyces hyalurolyticus, and cholesterol oxidase and cholesterol esterase from pseudomonas fluorescens were effectively adsorbed on an amberlite cg-50 column, when the cell-free cultured medium or the cultured medium with cell extract and without cell debris was applied without desalting but at ph less than or equal to 4.5. at the acidic ph, all the ion-exchange groups (-cooh) exist in the protonated form; the adsorption is not due t ... | 1982 | 6807971 |
| serology of aspergillus granuloma. | a total of 21 cases of granuloma caused by aspergillus species were encountered during the period 1972-79. the organs involved were nasal and paranasal sinuses, brain, orbit, subcutaneous tissue of check, lungs and endocardial valve in the decreasing order of their frequency. aspergillus flavus was the main etiological agent. immunodiffusion tests with various aspergillus species as antigen showed a positively of 17 out of 18 (94.4 per cent) cases. the role of precipitins in diagnosis and progno ... | 1982 | 6813743 |
| [isolation and identification of various bitter peptides from erythrocyte concentrates]. | | 1982 | 6814109 |
| [purification of exo-1,4-beta-xylosidase from aspergillus niger 15]. | from the preparation of xylanase of the fungus aspergillus niger 15 exo-1,4-beta-xylosidase was isolated by means of ethanol fractionation and chromatography on columns of sephadex g-50, cellulose de-52, sephadex sp c-50, sephadex g-200. the isolated enzyme (with the purification degree as calculated per protein 199, and yield with respect to activity 42.5%) was homogeneous during gel filtration, polyacrylamide gel electrophoresis in the presence and absence of na dodecyl sulfate, ultracentrifug ... | 1982 | 6815635 |
| [the use of a glucoamylase enzyme preparation obtained from aspergillus niger 16/132, for the hydrolysis of corn starch]. | | 1982 | 6816020 |
| a simple method of affinity chromatography for the purification of glucoamylase obtained from aspergillus niger c. | | 1982 | 6818051 |
| [the effects of various tetrahydro-1,3,5-thiadiazines as antifungal agents]. | | 1982 | 6820681 |
| aspergillus colonization associated with bilateral silicone mammary implants. | a case of aspergillus niger fungal colonization associated with bilateral inflatable silicone mammary implants is reported. painful fibrous capsular contractures without clinical evidence of infection or inflammation characterized the presenting symptoms. operative findings included a cheesy-white exudate that surrounded the implants and turbid fluid within the implants. all specimens yielded a heavy growth of aspergillus niger. special stains of the fibrous capsules were negative for fungal inv ... | 1983 | 6823489 |
| the active site of an acidic endo-1,4-beta-xylanase of aspergillus niger. | the substrate binding site of an acidic endo-1,4-beta-xylanase (1,4-beta-d-xylan xylanohydrolase, ec 3.2.1.8) of aspergillus niger was investigated using 1,4-beta-xylooligosaccharides (1-3h)-labelled at the reducing end. bond cleavage frequencies and v/km parameters of the oligosaccharides were determined under conditions of unimolecular hydrolysis and, according to the method of suganuma et al. (j. biochem. (tokyo) (1978) 84, 293-316), used for evaluation of subsite affinities. the substrate bi ... | 1983 | 6824697 |
| metabolism of resorcinol and salicylate in aspergillus niger. | | 1983 | 6825782 |
| antimicrobial activity of 3-methyl-5-furfurylidene-2-thioxo-4-thiazolidones. | substituted 3-methyl-5-furfurylidene-2-thioxo-4-thiazolidones 1-17 were tested for their action against bacillus pumilus, b. brevis and b. megaterium and against aspergillus flavus, a. fumigatus and a. niger. most of the compounds possessed an activity against both bacteria and fungi. these compounds were also tested for their effectiveness as virus inhibitors employing nicotiana glutinosa as assay host against tobacco mosaic virus. most of them exhibited potential antiviral activity both in vit ... | 1983 | 6832657 |
| marijuana smoking and fungal sensitization. | the possible role of marijuana (mj) in inducing sensitization to aspergillus organisms was studied in 28 mj smokers by evaluating their clinical status and immune responses to microorganisms isolated from mj. the spectrum of illnesses included one patient with systemic aspergillosis and seven patients with a history of bronchospasm after the smoking of mj. twenty-one smokers were asymptomatic. fungi were identified in 13 of 14 mj samples and included aspergillus fumigatus, a. flavus, a. niger, m ... | 1983 | 6833678 |
| colorimetric immunoassays using flavin adenine dinucleotide as label. | | 1983 | 6855621 |
| effect of vitamins on microbial production of citric acid by aspergillus niger. | the effect of different concentrations of ascorbic acid, biotin, p-aminobenzoic acid (paba), riboflavin and thiamine on the growth and activity of aspergillus niger strain al 29 was studied at different incubation periods. it was observed that ascorbic acid and paba inhibited the growth and activity of the mould, while the presence of thiamine and riboflavin stimulated the citric acid formation to the extent of 59.09% and 50.36%, respectively. however, biotin was found to be most suitable for in ... | 1982 | 6983793 |
| conjugation of enzymes to immunoglobulins using dimaleimides. | a method is described for coupling enzymes to immunoglobulins using sulphydryl derivatives of the proteins and a dimaleimide which is relatively water-soluble. parameters affecting the performance of the conjugates have been examined including level of sulphydryl incorporation, ratio of enzyme/immunoglobulin and nature of dimaleimide used. peroxidase-immunoglobulin conjugates made by the dimaleimide method have been compared with those made by the periodate oxidation method and found to have a s ... | 1980 | 6988011 |
| [ocular mycoses as a complication of hormonal therapy]. | | 1980 | 6989089 |
| antimicrobial activities of some antineoplastic and other withanolides. | | 1980 | 6994645 |
| [role of microorganisms in the destruction of spodumene]. | a broad spectrum of microorganisms has been shown to be involved in the destruction of spodumene, a typical mineral of lithium pegmatites. the following microorganisms are most active: the microscopic fungi penicillium notatum and aspergillus niger, the thiobacilli thiobacillus thiooxidans, and the so-called "silicate" slime forming bacterium bacillus micilaginosus n. sp. siliceus. spodumene destruction is accompanied with lithium, aluminium and silicon being transferred into solution. the activ ... | 1980 | 6995818 |
| immunogenic properties of pr8 influenza virus after treatment with acid protease. | brief exposure of pr8 influenza virus at ph 4.5 to extracts of aspergillus niger abolished the red cell agglutinating and infectious capacities of the virus without significantly decreasing its antigenicity or immunogenicity. the results of gel electrophoresis indicated that the ha1 portion of the viral hemagglutinin had been degraded by treatment of virus with aspergillus extract. the immunogenicity in mice of virus that had been exposed to a. niger extract appeared to be very broad, since vacc ... | 1980 | 7009489 |
| the effect of calcium on protoplast release from species of aspergillus. | the effect of various osmotica and lytic enzyme treatments, on the release of stable protoplasts from aspergillus niger and two strains of aspergillus fumigatus, has been investigated. most osmotica supported protoplast release at a concentration of 0.6 m except for cacl2 and ca(no3)2. supplementation of 0.6 m kcl with 0.2 m cacl2 enhanced release from both species, but depended upon strain of organism, concentration of osmoticum and enzyme system employed. more protoplasts were released at low ... | 1980 | 7010082 |
| survey of human pathogenic actinomycetes and fungi in soil from rome and other italian areas. | as part of a study sponsored by the ministry of health of italy, a research program on pathogenic actinomycetes, keratinophilic and pathogenic fungi in soil was carried out. two hundred soil samples, collected from different areas of the city of rome, calabria, emilia romagna, latium, apulia, sardinia, sicily, tuscany and umbria, were examined by several techniques to detect the widest possible variety of pathogenic actinomycetes and fungi. seven isolates of nocardia asteroides, four of actinoma ... | 1981 | 7012635 |
| isolation of protoplasts from aspergillus nidulans conidiospores. | protoplasts were prepared from conidiospores of aspergillus nidulans. the mononucleated conidia gave protoplasts of a uniform size, approximately 5-micron diameter, depending on the strain and the stabilizing medium used. conidia were preincubated with 2-deoxy-d-glucose in a minimal medium at 37 degrees c for 3 h. the swollen conidia were collected, resuspended in a buffer containing 0.4 m (nh4)2so4 as stabilizer, and incubated with oerskovia lytic enzymes at 30 degrees c for 3 or 4 h. approxima ... | 1981 | 7016284 |
| proteolytic action of aspergillus niger extract on influenza virus. | | 1980 | 7016806 |
| [coordination immobilization of fungal proteinases]. | the possibility of coordination immobilization of fungal enzymes from aspergillus niger mb and aspergillus flavipes on the styrene copolymer with maleic acid has been explored. optimal conditions for preparing insoluble chromium-containing enzyme polymeric complexes have been studied. the synergistic effect of salts of different metals on stability of chromium-containing immobilized preparations has been demonstrated. properties of the resultant immobilized preparations have been examined. | 1981 | 7017673 |
| mutagenicities of 61 flavonoids and 11 related compounds. | the mutagenicities of 61 flavonoids (naturally occurring flavonoid aglycones and flavonal glycosides and synthetic flavonoids) and those of 11 compounds structurally related to flavonoids were tested with salmonella typhimurium strains ta100 and ta98. among the 22 flavone derivatives tested, only wogonin was strongly mutagenic, while five derivatives, apigenin triacetate, acacetin, chrysoeriol, pedalitin, and pedalitin tetraacetate, were only weakly mutagenic. two bisflavonyl derivatives, neithe ... | 1981 | 7021146 |
| purification and kinetic characterization of mannitol-1-phosphate dehydrogenase from aspergillus niger. | | 1981 | 7030216 |
| the influence of some microorganisms on the in vitro multiplication of histomonas meleagridis. | | 1980 | 7032080 |
| [otomycosis in the south of chile]. | | 1981 | 7034110 |
| antimicrobial activity of carbazole derivatives. | | 1982 | 7047201 |
| some comparative studies of two alkaline phosphatases from aspergillus niger. | | 1982 | 7056363 |
| contribution to the fungal flora of cereal grains in egypt. | 30 genera and 77 species, in addition to 4 varieties were isolated from 25 samples of each of barley, wheat, maize and sorghum grains collected from different places in egypt. the broadest spectrum of genera and species was recorded in wheat (25 genera and 59 species + 4 varieties) followed by barley (21 genera and 52 species + 2 varieties), sorghum (14 genera and 33 species + 2 varieties) and maize grains (11 genera and 29 species + 2 varieties). aspergillus, fusarium, penicillium, and rhizopus ... | 1982 | 7070479 |
| aflatoxin and aflatoxicosis. i. fungal flora of some food and animal feeds with special references to aflatoxin-producing abilities. | a wide range of fungi, amounting to fifty-six species belonging to twenty-two genera, have been recovered from animal feeds and foodstuffs. the most frequent fungi were aspergillus niger, and a. flavus, followed by mucor racemosus, alternaria alternata. rhizopus stolonifer, penicillium corylophilum and p. notatum. three genera were found to be of moderate occurrence, namely, mucor, rhizopus and alternaria. the three following genera were of low occurrence: cladosporium, fusarium, and neurospora. ... | 1982 | 7070483 |
| [leaching of uranium containing phosphorites with heterotrophic microorganisms]. | the problem of heterotrophic leaching of metals was tested with uranium-containing phosphorites under laboratory conditions with regard to possible technical applications. as leaching agents we used the acids of citric and lactic acid fermentation, carried out with a strain of aspergillus niger in sulfite liquor under different conditions and with lactic acid bacteria in wheye. up to 12% uranium were soluted with citric acid fermentation of marocco phosphorite containing 153 ppm u in aspergillus ... | 1982 | 7072261 |
| frequency of clinical isolation and winter prevalence of different aspergillus species at a large southwestern army medical center. | the frequent occurrence of aspergillosis caused by species other than aspergillus fumigatus at brooke army medical center prompted the authors to study the prevalence of the different aspergillus species at brooke army medical center and at two other hospitals in san antonio, texas. two parameters were analyzed: (1) frequency of isolation from clinical specimens over a five-year period and (2) relative prevalence in the atmosphere during the late fall and early winter using volumetric air-sampli ... | 1982 | 7073023 |
| reaction pathways of substrate degradation by an acidic endo-1,4-beta-xylanase of aspergillus niger. | an acidic endo-1,4-beta-xylanase (1,4-beta-d-xylan xylanohydrolase, ec 3.2.1.8) of aspergillus niger catalyzes degradation of linear 1,4-beta-xylooligosaccharides by multiple reaction pathways analogous to those catalyzed by lysozyme and alpha-amylases. quantitative product analysis of enzyme-substrate mixtures using 1-3h-reducing end-labeled xylooligosaccharides and [u-14c]xylotriose led to the following conclusions: (1) bond cleavage frequencies of xylotriose, xylotetraose and xylopentaose are ... | 1982 | 7093285 |
| degradation of benzoate & salicylate by aspergillus niger. | | 1982 | 7106859 |
| cellulase enzyme from aspergillus niger. | cellulase enzyme was produced by a selected strain of aspergillus niger isolated from deteriorated wood and grown on different carbon sources. filter paper gave the highest yield, followed by carboxymethyl cellulose (cmc). cellobiose as well as glucose gave a low yield, while the yield from lactose was negligible. the concentration of filter paper cellulose that induced the maximum yield of the enzyme was 1%. both soluble cellulose (cmc) and cotton cellulose treated with phosphoric acid (swollen ... | 1982 | 7132786 |
| effect of changed cultivation conditions on the morphology of aspergillus niger and on citric acid biosynthesis in laboratory cultivation. | | 1982 | 7141333 |
| effect of aeration and agitation on the biosynthetic activity of diffusely growing aspergillus niger. | a considerable lowering of aeration demands occurs during diffuse growth of citric acid-producing aspergillus niger in a submerged cultivation. however, the diffuse culture poses stricter demands on the type of aeration and agitation. the impeller frequency affects considerably the morphology of the producer fungus and the accumulation of citric acid. the effect of impeller frequency on the distribution of air in the medium and on the amount of air fed into the diffuse culture is less important. | 1982 | 7141334 |
| aspergillus niger van tieghem mannosylation: polyprenylphosphate mannosyltransferase specificity. | aspergillus niger van tieghem microsomes contain an enzyme that catalyzes mannose transfer from gdp-mannose to polyprenylphosphate. the studies of the specificity of this enzyme for both the sugar donor (nucleoside diphosphate sugar) and the acceptor (polyprenylphosphates that were made available to the enzyme by means of the fusion of acceptor-loaded liposomes with the microsomal membranes) gave the following results. i) all the polyprenylphosphates from c15 to c120 were acceptors except retiny ... | 1982 | 7142813 |
| properties of aspergillus niger catalase. | catalase from aspergillus niger was purified to homogeneity as judged from the results of ultracentrifugation and polyacrylamide gel electrophoresis. the enzyme had a molecular weight of 385,000 as estimated from sedimentation measurements. carbohydrate analyses showed that the catalase was a glycoprotein containing about 8.3% neutral sugar and 1.9% glucosamine. under denaturing conditions, polyacrylamide gel electrophoresis revealed only one band with a molecular weight of 97,000 daltons in gel ... | 1982 | 7153210 |
| [use of a strain of aspergillus niger for purification of a hederagenin rhamnoglucoside from tubers of ficaria(ficaria ranunculoides moench)]. | | 1982 | 7158960 |
| [hydrazide derivatives of fluorobenzoic acids and their antimicrobial activity]. | the compounds of formula (a) were prepared by reaction between rn-substituted benzoyl hydrazides and r-substituted benzoyl chlorides; the compounds of formula (b) were obtained by condensing r-substituted benzoyl chlorides with isonicotinic hydrazide. all the compounds were tested for in vitro activity against five gram+ bacteria (s. aureus, s. epidermidis, b. subtilis, b. anthracis, m. paratuberculosis atcc 607), seven gram-bacteria (s. paratyphi a, s. paratyphi b, e. coli, b. abortus, b. melit ... | 1982 | 7168787 |
| [localised aspergillosis of the lung caused by aspergillus niger]. | a 51-year old man with a history of pulmonary tuberculosis suffered an exacerbation without demonstration of tubercle bacilli. the mycological examination of sputum specimens discovered an infection with aspergillus niger. diagnosis was confirmed by the pure culture of this fungus in bronchial mucus and the cultural and histological identification in surgical material. as a histological feature strong double-refractile crystalline material was found in fungi and surrounding lung tissue (wall of ... | 1982 | 7184222 |
| 'an intracavitary fungus ball composed of syncephalastrum'. | | 1980 | 7189103 |
| [interrelationship between fungi used as test cultures with regard to methods of testing the biostability of products of medical technology]. | | 1980 | 7189419 |
| glucose oxidase contains a disubstituted phosphorus residue. phosphorus-31 nuclear magnetic resonance studies of the flavin and nonflavin phosphate residues. | | 1981 | 7213598 |
| multiple forms of glucose oxidase with different carbohydrate compositions. | a glucose oxidase (beta-d-glucose:oxygen 1-oxidoreductase, ec 1.1.3.4) sample purified from aspergillus niger by a previously reported method was subjected to isoelectric focusing and gel electrophoresis, and found to be composed of at least six component enzymes. the isoelectric points of the component enzymes ranged from ph 3.9 to 4.3. analyses of the enzymes indicated that they all possess an identical protein moiety, since the amino acid compositions, the c-terminal sequences, the catalytic ... | 1981 | 7213749 |