| the parasitism of the mould penicillium purpurogenum on aspergillus niger. iv. the limitation of parasitic action. | | 1967 | 6056767 |
| degradation of salicylate by aspergillus niger. | | 1967 | 6057252 |
| the conversion of tryptophan to 2,3-dihydroxybenzoic acid and catechol by aspergillus niger. | | 1967 | 6064579 |
| a possible role of hydroxypyruvic acid reductase in growth of a. niger. | | 1967 | 6066161 |
| o-pyrocatechiuc acid carboxy-lyase from aspergillus niger. | | 1967 | 6066253 |
| the isolation and characterization of asperenone, a new phenylpolyene from aspergillus niger. | | 1967 | 6073032 |
| steroids and other factors influenceing the accumulation of asperenone and fermentation acids by aspergillus niger in replacement cultures. | | 1967 | 6073033 |
| genetic analysis by means of the parasexual cycle in aspergillus niger. | | 1967 | 6075182 |
| recovery of native proteins from preparative electrophoresis gel slices by reverse polarity elution. | a technique for high yield recovery of native, biologically active proteins from preparative polyacrylamide gel slices by reverse polarity elution is described. no apparatus other than the standard slab gel electrophoresis system is required. several proteins have been recovered in biologically active form at a 90% yield, in quantities ranging from 0.4 mg to 4.2 mg. the method is effective with both small (9,000 dalton) and large (186,000 dalton) polypeptides. both simple and complex proteins ar ... | 1984 | 6091091 |
| antifungal properties of 2-n-alkyn-1-ols. | fourteen 2-n-alkynols (c3-c14, c16, and c18) were tested against aspergillus oryzae, aspergillus niger, trichoderma viride, and myrothecium verrucaria in sabouraud dextrose agar at ph 5.6 and 7.0. toxicity to candida albicans, candida tropicalis, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. fungitoxicity was strongly influenced by chain length, slightly by the ph of the medium, and significantly b ... | 1984 | 6098642 |
| high-productivity alcohol fermentations using zymomonas mobilis. | a process is under development at the university of new south wales to produce fermentation ethanol faster and more efficiently. the process is based on the micro-organism zymomonas mobilis, which has higher specific rates of ethanol production and higher yields when compared with the traditionally used yeasts. by using hollow fibre membranes for cell recycle, high productivity continuous processes have been studied at laboratory-scale. pilot scale evaluations (500 litres) are now in progress th ... | 1983 | 6100832 |
| effect of amino acids on microbial production of citric acid by aspergillus niger. | the effect of different concentrations of cysteine, serine, lysine, aspartic acid, and glutamic acid on the growth and fermenting activity of aspergillus niger al 29 was studied at different incubation periods. it was observed that the presence of glutamic acid and aspartic acid stimulated the citric acid production to the extent of 79.6% and 76.7%, respectively. lysine was effective in producing citric acid by 62%. however, serine could not influence the yield (50.4%) to a greater extent, while ... | 1982 | 6125070 |
| oxidation of anionic nitroalkanes by flavoenzymes, and participation of superoxide anion in the catalysis. | the reactivities of anionic nitroalkanes with 2-nitropropane dioxygenase of hansenula mrakii, glucose oxidase of aspergillus niger, and mammalian d-amino acid oxidase have been compared kinetically. 2-nitropropane dioxygenase is 1200 and 4800 times more active with anionic 2-nitropropane than d-amino acid oxidase and glucose oxidase, respectively. the apparent km values for anionic 2-nitropropane are as follows: 2-nitropropane dioxygenase, 1.61 mm; glucose oxidase, 16.7 mm; and d-amino acid oxid ... | 1984 | 6149727 |
| the preparation and partial characterization of antigenic fractions obtained from the mycelial walls of several aspergillus species. | extracts with immunological activity were prepared from aspergillus fumigatus, a. flavus, a. terreus, a. niger and a. nidulans. in each case crude mycelial wall was extracted with an aqueous solution of triton x-100 giving detergent-soluble material. further fractionation was achieved by removing the detergent from this solution; the resultant precipitate was removed by centrifugation, and the aqueous supernatant was used as a source of soluble antigens. the sensitivity of these preparations was ... | 1980 | 6157778 |
| microbial alpha-amylase production from low quality potatoes. | | 1980 | 6161891 |
| inhibition of human digestive enzymes by hydrogenated malto-oligosaccharides. | maltose, malto-oligosaccharides and their reduced sugar analogues have been tested as substrates and inhibitors of human salivary alpha-amylase and small intestine alpha-glucosidases. - maltotriose and maltotetraose as well as their reduced analogues inhibited competitively alpha-amylase. maltitol, maltotriitol and maltotetraitol were not hydrolyzed. - the small intestine maltases hydrolyzed maltitol at a very low rate, but maltotriitol was split almost at the same rate as maltotriose. these thr ... | 1981 | 6169675 |
| [histamine release from equine leucocytes provoked by fungal allergens]. | | 1982 | 6181953 |
| development of fluorescent-antibody reagents for demonstration of pseudallescheria boydii in tissues. | we prepared fluorescent-antibody reagents to detect and identify pseudallescheria boydii in tissue. antisera to broken mycelium and condidia (particulate antigens) and to culture filtrates (soluble antigens) of p. boydii were produced in rabbits. antisera and globulin fractions of the antisera were labeled with fluorescein isothiocyanate and evaluated for their ability to stain p. boydii in tissues. the conjugates were first tested with cultures of 25 p. boydii isolates and 26 heterologous fungi ... | 1983 | 6195179 |
| high-frequency conversion to a "fluffy" developmental phenotype in aspergillus spp. by 5-azacytidine treatment: evidence for involvement of a single nuclear gene. | transient exposure of mycelia from aspergillus niger and aspergillus nidulans to the cytidine analog 5-azacytidine, leading to no more than 0.3 to 0.5% substitution for cytosine by 5-azacytosine in a. nidulans dna, resulted in the conversion of a high fraction of the cell population (more than 20%) to a mitotically and meiotically stable "fluffy" developmental phenotype. the phenotypic variants are characterized by the developmentally timed production of a profuse fluffy network of undifferentia ... | 1983 | 6197627 |
| heparin formation and mechanism of its action as growth promoter. | heparin was prepared and purified by two methods. rf value, elemental and chemical analyses of our isolate coincided with the clinically used heparin. heparin stimulated mitotic activities in escherichia coli b and aspergillus ochraceus. this was evident from the increase in dna, rna, and protein synthesis. the mechanism of growth stimulation by heparin probably takes place as follows: heparin associates with nuclear protein, releasing dna from dnp and thus stimulates dna synthesis. heparin was ... | 1984 | 6202077 |
| glucoamylases g1 and g2 from aspergillus niger are synthesized from two different but closely related mrnas. | by the use of glucoamylase-specific synthetic oligodeoxyribonucleotides and molecular cloning of cdna synthesized from aspergillus niger total poly(a) + rna, the primary structure of the glucoamylase g1 mrna was determined. glucoamylase g1 is synthesized as a precursor of 640 amino acid residues containing a putative signal peptide of 18 residues, a short propeptide of six residues and the 616 residues long mature enzyme. in vitro translations of mrna and immunoprecipitations with glucoamylase-s ... | 1984 | 6203744 |
| two different types of intervening sequences in the glucoamylase gene from aspergillus niger. | one single glucoamylase gene could be identified in the chromosomal dna of aspergillus niger by southern blot analysis. this glucoamylase gene was isolated from a genomic library of a. niger dna. the glucoamylase gene is situated on a 2.5-kb ecori-ecorv fragment and contains five intervening sequences in the coding region. one 169-bp intron is involved in differential mrna processing leading to the two different glucoamylase enzymes g1 and g2; the other four introns are all very small ranging fr ... | 1984 | 6204865 |
| monoclonal antibodies to mitochondrial f1-atpase and oligomycin sensitivity conferring protein (oscp). tools for recognition of well conserved and essential antigenic sites. | the preparation of anti-oscp monoclonal antibodies is described for the first time. one of these antibodies prevents the activating effect of oscp in reconstitution experiments. these antibodies and antibodies previously obtained against the alpha- and beta-subunits of pig heart mitochondrial f1-atpase have been used to look for well conserved epitopes in various species. one anti-beta antibody can recognize all species tested while the anti-oscp antibodies only recognize the pig or beef enzyme. ... | 1984 | 6206858 |
| specificity of antigens from pathogenic aspergillus species. ii. studies with line immunoelectrophoresis. | somatic (mycelial) and metabolic (culture filtrate) antigens of aspergillus flavus, a. fumigatus, a. nidulans, a. niger and a. terreus were compared by line immunoelectrophoresis with sera from patients with allergic bronchopulmonary aspergillosis (abpa) or aspergilloma, or from immunized animals. number of lines observed when tested with human sera were similar for somatic and metabolic preparations of a. fumigatus, but up to 33 lines were present when both types of antigens were tested simulta ... | 1984 | 6209814 |
| [aspergillosis of the maxillary sinus]. | | 1981 | 6212375 |
| cell wall degradation in the autolysis of filamentous fungi. | a systematic study on autolysis of the cell walls of fungi has been made on neurospora crassa, botrytis cinerea, polystictus versicolor, aspergillus nidulans, schizophyllum commune, aspergillus niger, and mucor mucedo. during autolysis each fungus produces the necessary lytic enzymes for its autodegradation. from autolyzed cultures of each fungus enzymatic precipitates were obtained. the degree of lysis of the cell walls, obtained from non-autolyzed mycelia, was studied by incubating these cell ... | 1982 | 6219290 |
| hydroxamate siderophore production by opportunistic and systemic fungal pathogens. | it has been suggested that siderophores may function as virulence factors. there have been few studies on production of siderophores by opportunistic and pathogenic fungi. we examined siderophore production by absidia corymbifera, aspergillus niger, rhizopus arrhizus, rhizopus oryzae, blastomyces dermatitidis, histoplasma capsulatum, sporothrix schenickii, candida albicans, and trichophyton mentagrophytes. fungi were cultured at 37 and 27 degrees c in a chemically defined low-iron media (0.2 mic ... | 1983 | 6221998 |
| partial purification and regulatory properties of phosphofructokinase from aspergillus niger. | phosphofructokinase (ec 2.7.1.11) from a citric acid-producing strain of aspergillus niger was partially purified by the application of affinity chromatography on blue dextran--sepharose and the use of fructose 6-phosphate and glycerol as stabilizers in the working buffer. the resulting preparation was still impure, but free of enzyme activities interfering with kinetic investigations. kinetic studies showed that the enzyme exhibits high co-operativity with fructose 6-phosphate, but shows michae ... | 1983 | 6223622 |
| a spectrophotometric method for the determination of glucose-6-phosphatase activity. | | 1980 | 6244755 |
| synthesis and use of p-nitrophenyl-2-o-(alpha-l-fucopyranosyl)-beta-d-galactopyranoside for the rapid detection of substrate-specific alpha-l-fucosidases. | | 1981 | 6263129 |
| enzymatic properties of an acid carboxypeptidase from aspergillus niger var. macrosporus. | acid carboxypeptidase (peptidyl-l-amino-acid hydrolase, ec 3.4.16.1) isolated from aspergillus niger var. macrosporus was investigated in regard to its kinetic parameters for two synthetic substrates. the optimum ph of peptidase activity toward z-glu-tyr-oh was ph 3.0 km and kappa cat values were 4.0 . 10(-3) m and 270 s-1 at ph 3.0 and 30 degrees c. the optimal ph of esterase activity toward bz-arg-oet was ph 5.2 km and kappa cat for esterolytic activity were 6.1 . 10(-4) m and 1500 s-1 at ph 5 ... | 1981 | 6266488 |
| the effect of tunicamycin on secreted glycosidases of aspergillus niger. | | 1981 | 6271064 |
| effect of deglycosylation on the stability of aspergillus niger catalase. | | 1981 | 6275794 |
| structural characterization of neutral oligosaccharides of human midcycle cervical mucin. | it was previously shown that alkaline borohydride treatment of human midcycle cervical mucin releases a heterogeneous population of reduced neutral, sialylated, and sulfated oligosaccharides (yurewicz, e. c., and moghissi, k. s. (1981) j. biol. chem. 256, 11895-11905). three major neutral oligosaccharides were isolated with approximate compositions of fuc:gal:glcnac:n-acetylgalactosaminitol (galnacol) = 0:2:1:1 (a1), 1:2:1:1 (a2), and 2:2:1:1 (a3). they comprised roughly 21%, 13%, and 8% of huma ... | 1982 | 6277894 |
| restriction enzyme analysis of mitochondrial dna of members of the genus aspergillus as an aid in taxonomy. | restriction endonuclease patterns of mitochondrial dna from seven species of the genus aspergillus (a. nidulans, a. wentii, a. awamori, a. niger, a. oryzae, a. tamarii and a. echinulatus) have been compared in order to test the phylogenetic relationships between these species. the fraction of restriction fragments common between all pairs of species from this set ranged from 0 to 1. the use of this approach for the taxonomy of the genus aspergillus is discussed. the postulated phylogenetic relat ... | 1982 | 6281364 |
| [synthesis and properties of digitoxigenin-3 beta-o-alpha-l-arabinofuranoside]. | the synthesis of a cardenolide glycoside with a furanoide sugar component, digitoxigenin-3 beta-o-alpha-l-arabinofuranoside (4), is described for the first time. 4 was prepared by the reaction of digitoxigenin with 2,3,5-tri-o-benzoyl-alpha-l-arabinofuranosylchloride and fétizon-reagent in benzene/dioxan followed by debenzoylation with ammonia in dry methanol. compound 4 is cleaved by alpha-l-arabinofuranosidase (aspergillus niger k1) into digitoxigenin and l-arabinose. hydrolytic stability agai ... | 1982 | 6298840 |
| a physical map of the ribosomal dna repeat unit of aspergillus nidulans. | the ribosomal dna repeat unit of aspergillus nidulans has been cloned in pbr322 and a restriction map constructed. the genes coding for the 17s, 5.8s and 25s rrnas are found in blocks separated by a 1.7 kb spacer region, with the 5.8s rna gene lying between the genes for the two larger rnas. the total length of the repeat unit is 7.7 kb. the 5s rrna is not present in the repeat unit. | 1982 | 6299883 |
| [fungal flora in a hospital environment]. | the airborne fungal flora was studied in different departments of a hospital by using a slit sampler (casella, london) where the measured volume of air is impacted to the surface of culture medium contained in petri dishes. colonies were counted after 3 days incubation at 25 degrees c and 37 degrees c. many species of hyphomycetes were identified. the main fungi isolated were cladosporium sp (especially c. cladosporioides), penicillium sp., alternaria alternata, aspergillus sp. (a. flavus, a. ni ... | 1983 | 6312583 |
| zinc: requirements, bioavailabilities and recommended dietary allowances. | this review has approached zinc requirements, bioavailabilities and recommended dietary allowances from a historical view. for example, a requirement for zinc was first demonstrated for the microorganism aspergillus niger more than a century ago, although zinc has been recognized as a dietary nutrient for humans only within the last decade. dietary requirement was defined as that quantity of zinc which must be provided daily in order to meet the metabolic requirement. the degree of bioavailabili ... | 1983 | 6318226 |
| [use of catabolic repression in the selection of the glucoamylase-producer aspergillus niger]. | the effect of various carbon sources and camp on the glucoamylase synthesis in aspergillus niger was studied to find carbon sources repressed the enzyme synthesis and conditions for the selection of catabolite stable mutants. maltose at a concentration of 0.5% stimulated the glucoamylase synthesis, but at a concentration of 4% it repressed not only the enzyme synthesis but the growth of the parental strain on the agar medium. the more active mutant 66 was obtained as a result of treatment of asp ... | 1983 | 6320159 |
| survey, purification, and properties of sugar phosphate phosphohydrolase among microorganisms. | sugar phosphate phosphohydrolase was purified approximately 500- to 600-fold to apparent homogeneity from escherichia coli b, escherichia coli c, escherichia coli var. communior, escherichia acidilactici, enterobacter aerogenes, neisseria meningitidis, and saccharomyces cereviseae. the molecular weights of the enzyme as estimated by gel filtration ranged from 97 x 10(3) to 101 x 10(3). the enzyme was composed of two subunits with the same molecular weight which ranged from 50 x 10(3) to 52 x 10( ... | 1983 | 6322944 |
| in vitro activities of amphotericin b in combination with four antifungal agents and rifampin against aspergillus spp. | strains of aspergillus fumigatus, aspergillus flavus, and aspergillus niger were tested for in vitro susceptibility with a microtiter plate system in buffered yeast-nitrogen base and in buffered minimal essential medium. isolates were tested against amphotericin b, flucytosine, rifampin, ketoconazole, ici 153,066, and bay n 7133 and against combinations of amphotericin b with each of the other five drugs. combinations of amphotericin b and rifampin were the most active against all three species ... | 1984 | 6329088 |
| contaminated first-aid dressings: report of a working party of the phls. | in a collaborative study 12 public health laboratory service laboratories and the division of hospital infection, central public health laboratory, investigated the degree of contamination of standard dressings produced by manufacturers in india or in england by a comparison of the results of culture of 25 sterilized dressings with those of 25 untreated dressings. of the 38 batches of dressings made in india 27 (71%) were judged contaminant and another six could be so judged when bacillus specie ... | 1983 | 6339612 |
| [fungal flora in a hospital milieu]. | the airborne fungal flora was studied in different departments of an hospital by using a slit sampler (casella, london) where the measured volume of air is impacted to the surface of culture medium contained in petri dishes. colonies were counted after 3 days incubation at 25 degrees c and 37 degrees c. many species of hyphomycetes were identified. the main fungi isolated were cladosporium sp (especially c. cladosporioides), penicillium sp., alternaria alternata, aspergillus sp. (a. flavus, a. n ... | 1983 | 6343973 |
| lytic enzyme activity in autolysing mycelium of aspergillus niger. | we have studied changes in the activity of some lytic enzymes contained in mycelium of aspergillus niger in cultures relative to the autolytic phase of growth. acid phosphatase, polygalacturonidase and alpha-amylase activity reached its highest level (40.7, and 8 u/sample, respectively) at the initiation of the autolytic phase of growth. 1.3-beta-glucanase and beta-n-acetylglucosaminidase reached its highest level (3.5 and 2 u/sample, respectively) during the first days of autolysis. alkaline ph ... | 1983 | 6346702 |
| a thiol inhibitor produced by aspergillus niger. | a low molecular weight inhibitor of the thiol proteases papain and bromelain has been identified and partially purified from the culture filtrates of aspergillus niger. further studies have shown that this inhibitor reacts with compounds containing a free thiol group and as such it is capable of inhibiting other enzymes that contain a functional thiol group at their active site. | 1983 | 6348207 |
| aspergillus fumigatus and other thermotolerant fungi generated by hospital building demolition. | on 13 september 1981, a 51-year-old seven-story building within our hospital complex was demolished by explosives. the concern that this event might release large numbers of thermotolerant fungi (tf), potentially hazardous to immunosuppressed patients, led us to seal hospital windows and doors. the air-handling systems were also manipulated. concentrations of airborne tf, especially aspergillus fumigatus, were determined before and after demolition, using andersen and cassella air samplers with ... | 1983 | 6354086 |
| detoxification of pesticides by microbial enzymes. | | 1983 | 6357841 |
| [compatibility and efficacy of meditonsin]. | heavy metals, including mercury, have recently been in discussion in connection with ecology and therapy. as meditonsin represents a 0,004% solution of mercury cyanide the following were investigated: absorption, excretion, effect on the kidneys and the chemical behaviour of mercury cyanide and other mercury salts as well as the antimicrobial effectivity in in vitro tests. with the dose recommended for adults neither an increase of mercury ions nor an increased excretion of these could be detect ... | 1984 | 6370812 |
| interaction of aspergillus fumigatus spores and pulmonary alveolar macrophages of rabbits. | the in vitro and in vivo interaction of rabbit pulmonary alveolar macrophages (pam) and aspergillus fumigatus spores was studied. in vitro experiments showed that pam from normal rabbits failed to appreciably kill a. fumigatus spores in 4 hours, while a. flavus and a. niger spores were destroyed effectively. prior opsonization of the spores with normal rabbit serum, rabbit anti-a. fumigatus serum, complement or lung lavage fluid has no profound enhancing effect on the phagocytosis or killing of ... | 1984 | 6370839 |
| antigenic profile of some typical and septate phialide-strains of aspergillus fumigatus. | antigens from typical and septate phialide-strains of aspergillus fumigatus, a. fumigatus var. ellipticus, a. fischeri, a. flavus and a. niger were analysed by fused rocket and two-dimensional immunoelectrophoresis. depsite strain-to-strain variations, isolates of the two morphological forms of a. fumigatus showed a close similarity in their antigenic profile; the percentage of qualitative sharing ranged between 80% and 100%. the number of antigens shared by the isolates of a. fumigatus var. ell ... | 1984 | 6374936 |
| [biomass production and biological purification of distillation slops in a 2-step process]. | in order to lower the chemical demand (cod) of slops from cane molasses alcohol a treatment of two steps which allows the production of single cell protein of candida utilis and paecilomyces variotii has been performed. its use reduces the treatment cost. in the first step the slops without sterilization supplemented with ammonium sulphate (5 g.l-1) and dipotassium phosphate (0.5 g.l-1) was inoculated with c. utilis and p. variotii. the yield was 24 and 18 g.l-1 of dry biomass and cod reduction ... | 1983 | 6400760 |
| effects of soil moisture and temperature on preharvest invasion of peanuts by the aspergillus flavus group and subsequent aflatoxin development. | four soil temperature and moisture treatment regimens were imposed on florunner peanuts 94 days after planting in experimental plots in 1980. at harvest (145 days after planting), the incidence of the aspergillus flavus group and the aflatoxin concentration were greatest in damaged kernels. extensive colonization of sound mature kernels (smk) by the a. flavus group occurred with the drought stress treatment (56% kernels colonized); colonization was less in the irrigated plot (7%) and the drought ... | 1983 | 6402980 |
| [effect of acid alpha-glucosidase from animal tissues on structurally different oligosaccharides]. | | 1983 | 6403061 |
| aspergillomycosis in intensively cultured tilapia from kenya. | | 1983 | 6404044 |
| [properties of the exo-1,4-beta-xylosidase of aspergillus niger 15]. | properties of exo-1,4-beta-xylosidase from the fungus aspergillus niger 15 were investigated. the enzyme was homogeneous during gel filtration, electrophoresis in polyacrylamide gel in the presence and absence of na dodecyl sulfate, ultracentrifugation and isoelectric focusing. the enzyme had a temperature optimum at 70 degrees, ph optimum 3.8-4.0 for p-nitrophenyl-beta-d-xylopyranoside (p-npx), was stable at ph 3-8, retained its 100% activity for 1 hour at 50 degrees and 42% activity at 60 degr ... | 1983 | 6406997 |
| substrate specificity and other properties of the beta-d-galactosidase from aspergillus niger. | beta-d-galactosidase from aspergillus niger was purified by conventional techniques, including the repeated use of chromatography on hydroxylapatite. the final preparation represented a 112-fold purification, with a 22% yield. the specific activity of the purified enzyme was 72 mumol of d-galactose released/min/mg of protein, using p-nitrophenyl beta-d-galactopyranoside as the substrate. the substrate specificity of the enzyme was studied by using saccharides having structural linkages similar t ... | 1983 | 6409408 |
| effects of combinations of simulated acid rain and cadmium or zinc on microbial activity in soil. | there was little effect on the rate of co2 evolution from glucose-supplemented soil, adjusted to ph 3.2 with a 2:1 combination of h2so4 and fuming hno3, and concomitant additions of 100 or 1000 ppm cd or of 1000 or 10,000 ppm zn (as sulfates) were no more inhibitory than in soil untreated with acid. in soil adjusted to ph 2.8, the lag in co2 evolution was increased by 1 day, and was extended further by the concomitant addition of 10,000 but not 1000 ppm zn or of 1000 but not 100 ppm cd. the grow ... | 1983 | 6411458 |
| antimicrobial activity of some thiol-containing heterocycles. | the minimum inhibitory concentration values for a group of synthesized heterocyclic systems were determined for gram-positive and gram-negative bacteria, yeast and fungi. the active compounds were screened further against a number of yeast and yeast-like organisms and gram-positive bacteria. the effect of two of these compounds on growth and mutagenicity was also tested. an attempt was made to correlate the structure of the compounds with their antimicrobial activity. | 1983 | 6413826 |
| [glucoamylase enzyme preparation from the culture broth of an aspergillus niger b77 strain. i. the isolation of the crude enzyme preparation and study of its properties]. | | 1983 | 6417985 |
| a rapid method for the determination of naringin, prunin, and naringenin applied to the assay of naringinase. | a naringinase assay capable of distinguishing between the content of naringin, prunin, and naringenin present in the incubation mixture, is described. the amount of these compounds can be estimated by combining two spectrophotometric procedures. (a) treatment with strong alkali to determine the amount of nargingenin as well as the sum of naringin and prunin. (b) assay of the liberated aldohexoses with o-aminodiphenyl. from the data thus obtained, the amount of the remaining substrate, the amount ... | 1983 | 6418025 |
| factors influencing the inhibition of aflatoxin production in corn by aspergillus niger. | aspergillus niger, a mold commonly associated with aspergillus flavus in damaged corn, interferes with the production of aflatoxin when grown with a. flavus on autoclaved corn. the ph of corn-meal disks was adjusted using naoh-hcl, citric acid-sodium citrate, or a water extract of a. niger fermented corn. aflatoxin formation was completely inhibited below ph 2.8-3.0, irrespective of the system used for ph adjustment. when grown in association with a. flavus nrrl 6432 on autoclaved corn kernels, ... | 1983 | 6418365 |
| actions of porcine pancreatic and bacillus subtilis alpha-amylases and aspergillus niger glucoamylase on phosphorylated (1--4)-alpha-d-glucan. | porcine pancreatic alpha-amylase (1,4-alpha-d-glucan glucanohydrolase, ec 3.2.1.1) produced o-6-phosphoryl-alpha-d-glucopyranosyl-(1--4)-o-alpha-d-glucopyranosyl -(1--4)-d-glucopyranose (6(3)-phosphoryl maltotriose) and o-alpha-d-glucopyranosyl-(1--4)-o-alpha-d-glucopyranosyl- o-3-phosphoryl-alpha-d-glucopyranosyl-(1--4)-d-glucopyranose (3(2)-phosphoryl maltotetraose) from potato starch upon exhaustive hydrolysis, while bacillus subtilis alpha-amylase (liquefying type) yielded o-alpha-d-glucopyr ... | 1983 | 6419777 |
| [resistance of mesospheric microorganisms to periodic freezing--thawing]. | the object of this work was to study cytological changes caused by periodic freezing-thawing in the conidia of the fungi aspergillus niger and penicillium chrysogenum isolated from the mesosphere as well as in the conidia of the same species taken from the collection of microorganisms. the conidia from the mesosphere were found to be highly resistant to the treatment. as was shown by electron microscopy, the outer "backbone" layer of the spore envelope broke down and the membranes of the plasmal ... | 1983 | 6422211 |
| [transglycosylation reactions catalyzed by aspergillus niger 15 beta-xylosidase]. | the products of transglycosylation formed as a result of action of beta-xylosidase from aspergillus niger 15 on p-nitrophenyl-beta-d-glucopyranoside and p-nitrophenyl-beta-d-xylopyranoside were studied by means of chromatography on sephadexes. they are formed at the substrate concentration of 10--100 mm with the amount 7--10 times less than that of hydrolysis products. peculiarities of chromatography of substrates, products of transglycosylation and p-nitrophenol on sephadexes g-15 and g-25 were ... | 1984 | 6422456 |
| pathogenic organisms in chronic suppurative otitis media in enugu, nigeria. | pathogenic organisms in chronic suppurative otitis media from 62 patients were identified. pseudomonas aeruginosa was responsible in 46%, staphylococcus aureus in 29%. proteus mirabilis in 13%, streptococcus pyogenes in 6%, aspergillus niger in 5% and mucor sp. in 2%. the most prevalent organism in children was staphylococcus aureus. the role of fungi in chronic suppurative otitis media is discussed. | 1983 | 6422594 |
| air-dried enzyme electrodes. | | 1984 | 6424506 |
| [rapid method for determining cellobiase activity]. | a simple and rapid method for determining the cellobiase activity in purified enzyme preparations was developed. it is based on a series of consecutive enzymatic reactions, i. e. hydrolysis of cellobiose by cellobiase, oxidation of the forming glucose by glucose oxidase, and formation of a dyed product under peroxidase action in the same reaction system. the dyed product is recorded spectrophotometrically at 460 nm. one measurement takes from 2-3 to 7-10 min depending on a particular method of t ... | 1984 | 6425819 |
| [action of desiccation and low temperatures on mesospheric strains of aspergillus niger and penicillium chrysogenum]. | aspergillus niger and penicillium chrysogenum strains were isolated from the mesosphere and characterised. their properties significant for migration in the atmosphere are discussed. the possibility of the anabiotic state of these fungi under the action of dehydration and low temperatures was studied as well as the degree of their resistance to the aforementioned extreme factors. | 1984 | 6429492 |
| the nucleotide sequences of the 5 s rrnas of seven molds and a yeast and their use in studying ascomycete phylogeny. | the sequences of the 5 s rrnas isolated from 8 ascomycete species belonging to the genera aspergillus, penicillium, acremonium and candida are reported. two of the examined strains each yielded a mixture of 3 slightly different 5 s rnas, which were individually sequenced after fractionation. a previously published sequence for aspergillus nidulans 5 s rna was found to contain errors. reconstruction of an evolutionary tree based on 5 s rna sequences showed that the 16 presently examined ascomycet ... | 1984 | 6429642 |
| [action of dehydration on the survival, morphology and ultrastructure of the conidia of air-borne and culture collection strains of aspergillus niger and penicillium chrysogenum]. | the effect of dehydration on the morphology and fine structure of conidia was studied with the atmospheric and collection strains of aspergillus niger and penicillium chrysogenum. dehydration did not cause changes in the structure of most conidia. however, destructive changes in the conidial wall and membrane were found in some conidia. the cytological changes of conidia were either reversible or irreversible, which determined their survival rate during dehydration. | 1984 | 6431241 |
| [microbial amine oxidase]. | | 1984 | 6431495 |
| [hospital aspergillosis]. | | 1984 | 6431721 |
| enzyme replacement therapy for primary adult lactase deficiency. effective reduction of lactose malabsorption and milk intolerance by direct addition of beta-galactosidase to milk at mealtime. | the addition of microbial beta-galactosidases directly to milk at mealtime represents a potential "enzyme replacement therapy" for primary lactase deficiency. we used the hydrogen breath test as the index of incomplete carbohydrate absorption to assess the efficacy of two enzymes--one from yeast, kluyveromyces lactis (lactaid), and the other from the fungus aspergillus niger (lactase n)--to assist in the hydrolysis of 18 g of lactose in 360 ml (12 oz) of whole milk when consumed by an adult lact ... | 1984 | 6434367 |
| fungal glucoamylases. | glucoamylase (alpha-1,4-glucan glucohydrolase, ec 3.2.1.3) from fungal sources is one of the microbial glycoproteins that has received considerable attention particularly because it is used in the commercial production of dextrose. several investigators have isolated glucoamylase from various fungal sources. in many instances the presence of more than one form of enzyme is common. the enzymes from most sources have ph optima between 4 and 5 and exhibit maximum activity between 40 and 60 degrees ... | 1983 | 6434506 |
| beta-xylosidase from aspergillus niger 15: purification and properties. | homogeneous (as judged by data from gel filtration, ultracentrifugation, polyacrylamide gel electrophoresis with and without sodium dodecyl sulfate (sds), and isoelectric focusing) beta-xylosidase showing beta-d-xylosidase, beta-d-glucosidase, beta-d-galactosidase, and alpha-l-arabinosidase activities has been isolated from the hemicellulase preparation of the microscopic fungus aspergillus niger 15 by ethanol fractionation and chromatography on sephadex g-50, cellulose de-52, and sephadexes sp ... | 1983 | 6434508 |
| structural studies on the o-glycosidically linked carbohydrate chains of glucoamylase g1 from aspergillus niger. | glucoamylase g1 from aspergillus niger contains an unusual type of carbohydrate-protein linkage, involving mannose o-glycosidically linked to serine and threonine. the majority of the neutral oligosaccharides of glucoamylase g1 are located in a region of about 70 amino acid residues which carries about 35 oligosaccharide units [(1983) carlsberg res. commun. 48, 517-527]. structural analysis was performed on the o-linked carbohydrates of a tryptic fragment from glucoamylase g1 comprising the segm ... | 1984 | 6439561 |
| enzymatic hydrolysis of lactose in a hollow-fiber reactor. | | 1984 | 6441497 |
| enzyme stability and glucose inhibition in cellulose saccharification. | | 1984 | 6441501 |
| [fatty acid composition of the lipids in fungi of the genus aspergillus developing on mineral media with different carbon sources]. | this work was aimed at studying the effect of different carbon sources in the composition of mineral media on the growth of fungi belonging to the aspergillus genus and on the fatty acid composition of their lipids. a chemically-defined medium with glucose was shown to be optimal for the growth of 18 aspergillus strains and for the synthesis of lipids by them. the fatty acid composition of lipids was studied when the fungi grew in media with different carbon sources. the lipids were shown to con ... | 1984 | 6442390 |
| releasing of intracellular glucoamylase from aspergillus niger. | a method is presented for the release of glucoamylase (alpha-d-1,4-glucan glucohydrolase, e. c. 3,2,1, 3) from mycelia of aspergillus niger. the process involves (a) suspension of mycelia at the stationary phase in water, and (b) homogenization and agitation of the suspension. the released intracellular enzyme is further purified by deae-cellulose. | 1984 | 6442427 |
| [otomycosis: etiology and analysis of predisposing factors]. | an otomycosis was found in 80 cases of external otitis on a total of 132. itching was the characteristic beginning, rapidly followed by pain, conductive hearing loss, tympanic perforation. aspergillus was isolated in 81.7% of cases: in particular, a. niger in 67.1% of cases. a. flavus in 13.4% a. fumigatus in 1.2%. candida albicans was isolated in 11% of cases. a statistical analysis based on chi 2 test was performed to evaluate the role of possible predisposing factors. highly significant facto ... | 1984 | 6442867 |
| drug therapy of aspergillus otitis externa. | | 1980 | 6451857 |
| [in vitro comparative study of the sensitivity of aspergillus to antifungal agents]. | minimum inhibitory concentrations (mic) of amphotericin b, 5-fluorocytosine, miconazole, tioconazole, econazole and ketoconazole were determined for 310 aspergillus strains belonging to four different species isolated from clinical specimens. econazole exhibited the best in vitro activity with mic less than or equal to 3.12 micrograms/ml for 96% of strains and less than or equal to 1.56 micrograms/ml for 68%. for amphotericin b, 69% of strains were less than or equal to 1.56 micrograms/ml and, f ... | 1984 | 6462761 |
| electrostatic control of enzyme reactions: the mechanism of inhibition of glucose oxidase by putrescine. | the interaction of putrescine dihydrochloride with glucose oxidase is reported. at ph 7.65 glucose oxidase is strongly anionic (z = -80). the pka of an essential acidic group on the reduced form of the enzyme is extremely sensitive to ionic strength, as predicted by simple electrostatic theory [j. g. voet, j. coe, j. epstein, v. matossian, and t. shipley (1981) biochemistry 20, 7182-7185]. putrescine dihydrochloride was found to inhibit glucose oxidase at ph 7.65 at a constant ionic strength of ... | 1984 | 6465905 |
| [antifungal properties of n-aryl-substituted maleaminic acids, their imides and copper salts]. | the influence of 39 compounds (n-aryl-maleiminic acids, n-aryl-maleinimid and cu-n-arylmaleiminic acids) on the growth of aspergillus niger, a. terreus and penicillium cyclopium has been studied. maleimides have been shown to have the more toxic effect on the fungi. | 1984 | 6466746 |
| experimental evidence for the occurrence in honey of specific substances active against microorganisms. | numbers of micro-organisms in sewage, soil, air and tap-water counted on media containing honey were lower than those counted on the same media containing equivalent concentrations of the sugars known to occur in honey. similar experiments achieved with specific micro-organisms showed that honey had more pronounced inhibitory effects than the equivalent sugar solutions on salmonella sp., escherichia coli, aspergillus niger and penicillium chrysogenum, but not on bacillus subtilis and saccharomyc ... | 1984 | 6475343 |
| immobilized glucose oxidase in the potentiometric detection of glucose. | previous work has shown that glucose oxidase can be immobilized on platinum to give an electrode that responds potentiometrically to glucose over the clinically useful range of about 10-250 mg glucose/100 ml. the present studies were carried out with electrochemically pretreated platinum and with gold or porous graphite substituted for the platinum support. the presence of the enzyme gave a significantly enhanced potentiometric response over that obtained with the bare support for both the pretr ... | 1984 | 6476822 |
| biological activity of 4-hydroxyisophthalic acid derivatives. hydrazones with antimicrobial activity. | the following hydrazono derivatives (i-xix) of type (a) (sequence in text) where rn = (sequence in text ) (i-xvii); (sequence in text) (xviii); -ccl3 (xix); and xn = h (i); 2-cl (ii); 3-cl (iii); 4-cl (iv); 2-no2 (v); 3-no2 (vi); 4-no2 (vii); 2-oh (viii); 3-oh (ix); 4-oh (x); 4-f (xi); 3,4-och3,oh (xii); 3,4,5-och3,oh,j (xiii); 3,4-och3,och3 (xiv); 2,4-cl2 (xv); 3,4-cl2 (xvi); 2,6-cl2 (xvii); were prepared and characterized in an attempt to make available for testing a representative selection o ... | 1984 | 6477732 |
| isolation of aneuploid-generating mutants of aspergillus nidulans, one of which is defective in interphase of the cell cycle. | a method is described for isolating mutants potentially defective in loci involved in mitotic chromosome segregation. conditional lethal, heat-sensitive (42 degrees ) mutants were assayed at a subrestrictive temperature of 37 degrees for an inflated production of colonies displaying phenotypes and behavior patterns of whole chromosome aneuploids. of 14 mutants, three showed specificity for one disomic phenotype, whereas 11 generated colonies mosaic for different aneuploid phenotypes. this latter ... | 1984 | 6479583 |
| conversion of cassava starch to biomass, carbohydrates, and acids by aspergillus niger. | the filamentous fungus, aspergillus niger, efficiently converted cassava polysaccharides to mycelial mass, simple sugars, and acids during the course of its growth. a typical 70-ml culture broth containing 2% cassava polysaccharides yielded 0.38 g dry mycelial mass, 1.14 mmol reducing sugars, and 1.17 meq acids at the end of 42 h. about 70% of the initial total carbohydrate in the medium was degraded during the same period. the sugars and acids in the culture broths were analyzed by hplc on a si ... | 1984 | 6490584 |
| evaluation of an indirect hemagglutination inhibition technique for identifying antibody in animals experimentally and naturally exposed to fungi. | rabbits were exposed to aerosols containing spores of aspergillus fumigatus, aspergillus flavus, aspergillus niger, or of a penicillium sp. sera from these rabbits were tested by indirect hemagglutination (iha) and by iha inhibition. the serologic reactions with the rabbit sera were compared to reactions with sera from cattle naturally exposed to airborne microorganisms. by three months of age, most cattle had positive iha reactions to a. fumigatus and penicillium antigens. the iha inhibition te ... | 1984 | 6493314 |
| anthranilate hydroxylase from aspergillus niger: new type of nadph-linked nonheme iron monooxygenase. | anthranilate hydroxylase from aspergillus niger catalyzes the oxidative deamination and dihydroxylation of anthranilic acid to 2,3-dihydroxybenzoic acid. this enzyme has been purified to homogeneity and has a molecular weight of 89,000. the enzyme is composed of two subunits of 42,000 with 2 gram-atoms of nonheme iron per mol. fe2+-chelators like alpha,alpha'-dipyridyl and o-phenanthroline are potent inhibitors of the enzyme activity. absorption and fluorescence spectra of the enzyme offer no ev ... | 1984 | 6501219 |
| isolation of pathogenic aspergillus species from commercially-prepared potting media. | twelve commercially-prepared potting soils were screened for the presence of pathogenic aspergillus species. pathogenic aspergillus species were isolated from 67% of the soils. a fumigatus was isolated from 42% and a. flavus and a. niger from 33%. | 1984 | 6513995 |
| necrotizing pulmonary aspergillosis with oxalosis. | | 1984 | 6521752 |
| enhancement of the in vitro activity of amphotericin b against aspergillus spp. by tetracycline analogs. | strains of aspergillus fumigatus, aspergillus flavus, and aspergillus niger were tested for in vitro susceptibility to amphotericin b alone and in combination with fixed concentrations of tetracycline, doxycycline, or minocycline, using buffered minimal essential medium in microtiter plates. enhanced inhibitory activity was seen, especially with combinations of amphotericin b and minocycline. synergistic activity between amphotericin b and minocycline was observed in each of five isolates of eac ... | 1984 | 6524900 |
| cationic degradation of streptomycin and kanamycin by aspergillus niger in broth cultures during fermentation in shake flasks. | | 1984 | 6533144 |
| phytoalexin synthesis in soybean cells: elicitor induction of phenylalanine ammonia-lyase and chalcone synthase mrnas and correlation with phytoalexin accumulation. | a glucan elicitor from cell walls of the fungus phytophthora megasperma f. sp. glycinea, a pathogen of soybean (glycine max), induced large and rapid increases in the activities of enzymes of general phenylpropanoid metabolism, phenylalanine ammonia-lyase, and of the flavonoid pathway, acetyl-coa carboxylase and chalcone synthase, in suspension-cultured soybean cells. the changes in phenylalanine ammonia-lyase and chalcone synthase activities were correlated with corresponding changes in the mrn ... | 1984 | 6540068 |
| biological activity of 4-hydroxyisophthalic acid derivatives. ii. anilides with antimicrobial activity. | a series of 1,3 -bis-anilides of 4-hydroxyisophthalic acid was prepared and tested for antibacterial and antifungal activity. the prepared compounds (i-xviii), of general structure (a), (formula: see text) where xn = h (i); 2-f (ii); 3-f (iii); 4-f (iv); 2-cl (v); 3-cl (vi); 4-cl (vii); 2-br (viii); 3-br (ix); 4-br (x); 2-j (xi); 3-j (xii); 4-j (xiii); 2,5-cl2 (xiv); 2,4-br2 (xv); 2,3,4-cl3 (xvi), 2,4,5-cl3 (xvii); 2,4,6-cl3 (xviii), were investigated for the purpose of determining the effect of ... | 1984 | 6543319 |
| a fungal polygalacturonase activating factor from tomato fruit. | | 1984 | 6545222 |