Publications

[obtaining pectinolytic mutants of aspergillus niger].		1980	7467410
an application of ellipsometry. assessment of polysaccharide and glycoprotein interaction with lectin at a liquid/solid interface.	lectins were specifically adsorbed from solution onto metallized glass slides coated with polysaccharide, glycopeptide and glycoprotein films. the degree of interaction was determined by measuring the thickness of the bound lectin layer with an ellipsometer after washing and drying the slide. the binding of concanavalin a (tetrameric) and succinyl concanavalin a (dimeric) to a yeast mannan film was studied as a function of lectin concentration, temperature, rinsing time and the extent of stirrin ...	1980	7417529
isolation, purification and partial characterization of alkaline phosphatase from aspergillus niger.		1980	7380079
influence of environmental factors on antagonism of fungi by bacteria in soil: nutrient levels.	the addition of 0.25, 0.5, or 1.0% glucose to a soil (k) amended with either 6% kaolinite (k6k) or montmorillonite (k6m) or the adjustment of the c/n ratio of the soils from 23/1 to 10/1 with nh(4)no(3) eliminated the inhibition of aspergillus niger by serratia marcescens, regardless of whether the fungus and bacterium were inoculated into the same or separate sites in the soils. the adjustment of the c/n ratio to 15/1 or of the c/p ratio from 1,000/1 to 100/1 with kh(2)po(4) did not eliminate t ...	1980	16345509
induction, isolation, and characterization of aspergillus niger mutant strains producing elevated levels of beta-galactosidase.	[this corrects the article on p. 594 in vol. 41.].	1981	16345782
citric acid fermentation by aspergillus niger on low sugar concentrations and cotton waste.	the possible use of cotton waste as a carbohydrate source of citric acid production by aspergillus niger was examined. no citric acid was produced when a. niger was grown on cotton waste as a sole carbon source. in two-stage fermentations, however, mycelium obtained from surface cultures in cotton waste medium yielded more citric acid when transferred to sucrose-containing media than when directly inoculated to sucrose-containing media. it is concluded that cotton waste can be used for saving su ...	1981	16345802
enzymatic and microbial preparation of d-xylulose from d-xylose.	a high-d-xylulose mixture (d-xylose-d-xylulose = 33:67) was prepared from the cold ethanol extract of preisomerized d-xylose solution (d-xylose-d-xylulose = 77:23). fusarium oxysporum f. sp. lini and aspergillus niger were demonstrated to preferentially utilize d-xylose in the mixture of d-xylose and d-xylulose. chromatographically pure d-xylulose was thus obtained in 90% yield. a high-d-xylulose mixture was also incubated with rhodotorula toruloides, klebsiella pneumoniae, candida utilis, or mu ...	1981	16345816
microbial transformation of beta-ionone and beta-methylionone.	aspergillus niger jts 191 was selected from many microorganisms tested as capable of converting ionones to other compounds having aromas. the individual transformation products from beta-ionone were isolated and identified by comparison with synthetically derived compounds. the major products were (r)-4-hydroxy-beta-ionone and (s)-2-hydroxy-beta-ionone. 2-oxo-, 4-oxo-, 3,4-dehydro-, 2,3-dehydro-4-oxo-, 3,4-dehydro-2-oxo-, (s)-2-acetoxy-, (r)-4-acetoxy-, and 5,6-epoxy-beta-ionone and 4-(2,3,6-tri ...	1981	16345730
[isolation of solasonine from glucoalkaloid mixtures by fungal fermentation.].	a strain of aspergillus niger grown on a medium containing 60% of solamargine and 40% of solasonine was able to hydrolyse selectively solamargine. it was thus possible to extract pure solasonine. different parameters influencing the speed of hydrolysis of the solamargine were examined.	1981	17402045
purification and properties of aldose 1-epimerase from aspergillus niger.	aspergillus niger atcc 6274 was selected as an aldose 1-epimerase (ec 5.1.3.3) producer from 45 stock cultures of a. niger. the aldose 1-epimerase was purified 115-fold to apparent homogeneity from cell extracts with a yield of 2.6%. the molecular weight was calculated to be 260,000 and that of the subunit to be 130,000. the enzyme preparation was active at ph 5-7. the km value was 50 mm and the v value was 1200 units/mg toward alpha-d-glucose. this enzyme catalyzed mutarotation of the following ...	1981	7317442
[comparative physiological and biochemical characteristics of aspergillus niger isolated from the mesosphere and of its mutant].	the aim of this work was to study certain physiological-biochemical characteristics of aspergillus niger, strain 26, isolated from the mesosphere as well as those of its mutant having light-brown conidia. the parent strain and its mutant were grown in a liquid chapek medium to study accumulation of the biomass, changes in the ph of the medium, as well as assimilation of glucose, nitrogen (no3-) and phosphorus (po4-). the content of polysaccharides, protein, rna and dna was determined in the biom ...	1981	7329352
bilateral endogenous aspergillus endophthalmitis.	a case of successfully treated aspergillus endophthalmitis is presented. treatment consisted of wide vitrectomy. at the end of surgery, 5 micrograms of amphoteracin b were injected into the center of the vitreous cavity, and an additional .75 mg was given subconjunctivally both at surgery and on the second postoperative day, at which time 2 mg of dexamethasone were also given subjunctivally. systemic antifungal agents were not used. after 2 1/2 months, the second eye developed a similar metastat ...	1981	7348830
[about the antimicrobial activity of substituted aromatic aldehydes (author's transl)].	the mic-values and the antimicrobial activity in the paper disc diffusion method of 86 resp. 79 substituted aromatic aldehydes have been investigated. antibacterial activity has been tested against staphylococcus aureus, pseudomonas aeruginosa, proteus vulgaris and klebsiella pneumoniae, the antifungal activity against trichophyton mentagrophytes, aspergillus niger and candida albicans. 13 classes of substituents have been investigated. the most active compounds are substituted with nitro-, hydr ...	1981	7257654
isolation, purification and some chemical properties of an acid carboxypeptidase from aspergillus niger var. macrosporus.	acid carboxypeptidase (peptidyl-l-amino-acid hydrolase, ec 3.4.16.1) was purified to a homogeneous state from the water extracts of koji cultures of aspergillus niger var. macrosporus. the molecular weight of the enzyme was determined to e 136 000 by sedimentation equilibrium method. the denatured specimen of the enzyme exhibited a molecular weight of 60 000 in the sedimentation equilibrium in 6 m guanidinium chloride, suggesting that the native enzyme is composed of two identical subunits. howe ...	1981	7260098
studies on air-borne fungi at qena. i. seasonal fluctuations.	73 species which belong to 24 genera were collected in 200 and 35 exposures made during the period may 1976-october 1977 at each of two levels (2 m and 20 m). the air-borne fungi showed seasonal periodicities and the highest incidence was recovered in spring and autumn and the least in the summer. aspergillus was the dominating genus. 17 species were collected at the two levels of which a. niger and a. flavus were the most common. other common genera were cladosporium which was represented by c. ...	1981	7269656
aspergillus niger.		1981	7293976
the role of oligosaccharide in transport of egg yolk riboflavin-binding protein to the egg.	the carbohydrate portion of chicken egg yolk riboflavin-binding protein was examined to determine its role in the biological activity of the protein. yolk rbp was found to contain 5--6 mannose, five galactose, 12 n-acetylglucosamine and four sialic acid residues. specific modifications of the oligosaccharide moiety were performed which included removal of sialic acid by mild acid hydrolysis, oxidation of galactose by galactose oxidase, and removal of n-acetylglucosamine and galactose residues by ...	1981	7295796
electrostatic control of enzyme reactions: effect of ionic strength on the pka of an essential acidic group on glucose oxidase.	the dissociation constant of an essential acidic group on the reduced form of glucose oxidase from aspergillus niger (k4) has been found to be extremely sensitive to ionic strength. increasing the ionic strength from 0.025 to 0.225 causes a decrease in pk4,obsd of 0.9 ph unit, from 8.2 to 7.3. analysis of the ionic strength dependence of pk4,obsd, making the assumption that the enzyme is a homogeneously charged impenetrable sphere [edsall, j. t., & wyman, j. (1958) biophysical chemistry, vol. 1, ...	1981	7317376
mode of elongation of the glycerol phosphate polymer of membrane lipoteichoic acid of streptococcus faecium atcc 9790.	specific degradation of membrane lipoteichoic acid of streptococcus faecium atcc 9790 by a phosphodiesterase from aspergillus niger and by periodate oxidation has demonstrated that the enzymatic synthesis of the glycerol phosphate polymer of the molecule occurs by an external elongation system. evidence of this type of mechanism was obtained with lipoteichoic acid synthesized in vivo or in vitro by differential radioisotope labeling techniques. the glycerol phosphate repeating units were transfe ...	1981	7240097
[effect of composition of multienzyme cellulase complexes on rate-limiting steps for non-soluble (native) cellulose hydrolysis].	it was shown that the kinetics of multienzyme cellulase complexes are generally characterized by the absence of any single rate-limiting step of hydrolysis, even when the rates of individual steps of the process are substantially different. this peculiarity of the kinetic behaviour of cellulases can be explained in terms of certain "shunt ways" in a series of individual steps of enzymatic degradation of cellulose into glucose. a basic kinetic scheme for the degradation of cellulose was developed ...	1981	7248368
glucose oxidase contains a disubstituted phosphorus residue. phosphorus-31 nuclear magnetic resonance studies of the flavin and nonflavin phosphate residues.		1981	7213598
[effect of local anesthetics on the transport of mannose in the microsomal membranes of aspergillus niger van tieghem].	the influence of various local anaesthetics has been studied on the mannose transfer enzymic system which is localized in aspergillus niger van tieghem microsomal membranes. the n-alkanes and tertiary amines do not seem to react on the first step of the reaction: i.e. the polyprenylphosphate-mannose biosynthesis. however two amines, the dibucaine and to a lesser extent the tetracaine, inhibit the mannose transfer from the polyprenylphosphate-mannose to the endogenous proteins.	1981	7226275
increased respiratory activity in a diploid strain of aspergillus niger (v35).		1981	7237283
inhibition of human digestive enzymes by hydrogenated malto-oligosaccharides.	maltose, malto-oligosaccharides and their reduced sugar analogues have been tested as substrates and inhibitors of human salivary alpha-amylase and small intestine alpha-glucosidases. - maltotriose and maltotetraose as well as their reduced analogues inhibited competitively alpha-amylase. maltitol, maltotriitol and maltotetraitol were not hydrolyzed. - the small intestine maltases hydrolyzed maltitol at a very low rate, but maltotriitol was split almost at the same rate as maltotriose. these thr ...	1981	6169675
oxalosis associated with an aspergillus niger fungus ball. report of a case.	during a seventeen day period an a. niger fungus ball evolved within a healed tuberculous cavity of a patient. symptoms were a cough with a chocolate brown expectoration and dyspnea. the patient died and necropsy was performed. crystals of calcium oxalate were deposited in the cavity lining and in the adjacent tissue of the lung. fibrosis, mononuclear infiltration and intraalveolar purulent exudate were seen in these tissues. some small vessels presented recent thrombosis and deposition of calci ...	1981	7219509
mutagenicities of 61 flavonoids and 11 related compounds.	the mutagenicities of 61 flavonoids (naturally occurring flavonoid aglycones and flavonal glycosides and synthetic flavonoids) and those of 11 compounds structurally related to flavonoids were tested with salmonella typhimurium strains ta100 and ta98. among the 22 flavone derivatives tested, only wogonin was strongly mutagenic, while five derivatives, apigenin triacetate, acacetin, chrysoeriol, pedalitin, and pedalitin tetraacetate, were only weakly mutagenic. two bisflavonyl derivatives, neithe ...	1981	7021146
purification and kinetic characterization of mannitol-1-phosphate dehydrogenase from aspergillus niger.		1981	7030216
[characterization of the cleaving mechanism of pectinesterase of aspergillus niger].		1981	7278946
[otomycosis in the south of chile].		1981	7034110
multiple forms of glucose oxidase with different carbohydrate compositions.	a glucose oxidase (beta-d-glucose:oxygen 1-oxidoreductase, ec 1.1.3.4) sample purified from aspergillus niger by a previously reported method was subjected to isoelectric focusing and gel electrophoresis, and found to be composed of at least six component enzymes. the isoelectric points of the component enzymes ranged from ph 3.9 to 4.3. analyses of the enzymes indicated that they all possess an identical protein moiety, since the amino acid compositions, the c-terminal sequences, the catalytic ...	1981	7213749
survey of human pathogenic actinomycetes and fungi in soil from rome and other italian areas.	as part of a study sponsored by the ministry of health of italy, a research program on pathogenic actinomycetes, keratinophilic and pathogenic fungi in soil was carried out. two hundred soil samples, collected from different areas of the city of rome, calabria, emilia romagna, latium, apulia, sardinia, sicily, tuscany and umbria, were examined by several techniques to detect the widest possible variety of pathogenic actinomycetes and fungi. seven isolates of nocardia asteroides, four of actinoma ...	1981	7012635
isolation of protoplasts from aspergillus nidulans conidiospores.	protoplasts were prepared from conidiospores of aspergillus nidulans. the mononucleated conidia gave protoplasts of a uniform size, approximately 5-micron diameter, depending on the strain and the stabilizing medium used. conidia were preincubated with 2-deoxy-d-glucose in a minimal medium at 37 degrees c for 3 h. the swollen conidia were collected, resuspended in a buffer containing 0.4 m (nh4)2so4 as stabilizer, and incubated with oerskovia lytic enzymes at 30 degrees c for 3 or 4 h. approxima ...	1981	7016284
[coordination immobilization of fungal proteinases].	the possibility of coordination immobilization of fungal enzymes from aspergillus niger mb and aspergillus flavipes on the styrene copolymer with maleic acid has been explored. optimal conditions for preparing insoluble chromium-containing enzyme polymeric complexes have been studied. the synergistic effect of salts of different metals on stability of chromium-containing immobilized preparations has been demonstrated. properties of the resultant immobilized preparations have been examined.	1981	7017673
estimation of lactose hydrolysis by freezing point measurements in milk and whey substrates treated with lactases from various microorganisms.	beta-galactosidase concentrates obtained from several microorganisms were used to hydrolyze skim milk, low fat (2%) milk, sweet whey, acid whey, acid whey permeate, and acid whey concentrate. among acid substrates, the freezing point depression for each 1% lactose hydrolyzed was the greatest with the lactase from aspergillus niger (0.0501 degrees h); among neutral substrates, the depression was greater in sweet whey (0.0495 degree h) and lesser in low fat milk (0.0445 degrees h). all data were s ...	1981	6796559
natural-abundance 13c-n.m.r. spectra of some model compounds for uncommon glycopeptide linkages in glycoproteins.		1981	6797730
fungal pneumonia (part 4): invasive pulmonary aspergillosis.		1981	6788456
[effect of dimilin on aspergillus niger growth and the formation of chitin].		1981	6791709
[enzymatic conversion of polymers. nature of apparent product inhibition in the course of enzymatic degradation of polymer substrates].	the general regularities for the kinetics of enzymatic conversion of polymers were studied. it was proposed that the formal kinetic analysis of progress kinetic curves for enzymatic degradation of polymers inevitably results in the apparent effects of product inhibition, even in the case when the products are not virtually bound to the enzyme and there is no real product inhibition at all. an experimental verification of this hypothesis was performed, using maltodextrins hydrolysis by aspergillu ...	1981	6796131
thermal stabilization of glucose oxidase and glucoamylase by physical entrapment.	physical entrapment was used as an approach to achieve thermal stabilization of enzymes. the t 1/2 values for the thermoinactivation of glucose oxidase and glucoamylase were increased several-fold by their entrapment in polyacrylamide gels. in polyacrylate gels the individual enzymes behaved differently, probably owing to microenvironmental effects arising by the polyelectrolyte nature of the carrier.	1981	6796045
determining the kinetic behaviour for the secretion of milk toxin as related to dosage level of aflatoxin b1.	39 species which belong to 14 genera were isolated from 110 milk samples collected from different dairy sources at assiut city. the most frequent fungi were a. niger, a flavus and clasdosporium cladosporioides, followed by curvularia spicifera and penicillium corylophilum. the remaining species were of rare occurrence. the fluorescence-method for detecting aflatoxin-producing strains demonstrated the presence of two isolates namely a. flavus possesses this property. one of these toxic isolates w ...	1981	6794233
biosynthesis of d-alanyl-lipoteichoic acid in lactobacillus casei: d-alanyl-lipophilic compounds as intermediates.	d-alanyl-lipoteichoic acid (d-alanyl-lta) from lactobacillus casei contains a poly(glycerol phosphate) moiety that is selectively acylated with d-alanine ester residues. to characterize further the mechanism of d-alanine substitution, intermediates were sought that participate in the assembly of this lta. from the incorporation system utilizing either toluene-treated cells or a combination of membrane fragments and supernatant fraction, a series of membrane-associated d-[14c]alanyl-lipophilic co ...	1981	6783617
induction, isolation, and characterization of aspergillus niger mutant strains producing elevated levels of beta-galactosidase.	an aspergillus niger mutant strain, vtt-d-80144, with an improvement of three- to fourfold in the production of extracellular beta-galactosidase was isolated after mutagenesis. the production of beta-galactosidase by this mutant was unaffected by fermentor size, and the enzyme was also suitable for immobilization.	1981	6784672
the effect of tunicamycin on secreted glycosidases of aspergillus niger.		1981	6271064
[aspergillosis of the maxillary sinus].		1981	6212375
enzymatic properties of an acid carboxypeptidase from aspergillus niger var. macrosporus.	acid carboxypeptidase (peptidyl-l-amino-acid hydrolase, ec 3.4.16.1) isolated from aspergillus niger var. macrosporus was investigated in regard to its kinetic parameters for two synthetic substrates. the optimum ph of peptidase activity toward z-glu-tyr-oh was ph 3.0 km and kappa cat values were 4.0 . 10(-3) m and 270 s-1 at ph 3.0 and 30 degrees c. the optimal ph of esterase activity toward bz-arg-oet was ph 5.2 km and kappa cat for esterolytic activity were 6.1 . 10(-4) m and 1500 s-1 at ph 5 ...	1981	6266488
effect of deglycosylation on the stability of aspergillus niger catalase.		1981	6275794
synthesis and use of p-nitrophenyl-2-o-(alpha-l-fucopyranosyl)-beta-d-galactopyranoside for the rapid detection of substrate-specific alpha-l-fucosidases.		1981	6263129
[histamine release from equine leucocytes provoked by fungal allergens].		1982	6181953
effect of amino acids on microbial production of citric acid by aspergillus niger.	the effect of different concentrations of cysteine, serine, lysine, aspartic acid, and glutamic acid on the growth and fermenting activity of aspergillus niger al 29 was studied at different incubation periods. it was observed that the presence of glutamic acid and aspartic acid stimulated the citric acid production to the extent of 79.6% and 76.7%, respectively. lysine was effective in producing citric acid by 62%. however, serine could not influence the yield (50.4%) to a greater extent, while ...	1982	6125070
restriction enzyme analysis of mitochondrial dna of members of the genus aspergillus as an aid in taxonomy.	restriction endonuclease patterns of mitochondrial dna from seven species of the genus aspergillus (a. nidulans, a. wentii, a. awamori, a. niger, a. oryzae, a. tamarii and a. echinulatus) have been compared in order to test the phylogenetic relationships between these species. the fraction of restriction fragments common between all pairs of species from this set ranged from 0 to 1. the use of this approach for the taxonomy of the genus aspergillus is discussed. the postulated phylogenetic relat ...	1982	6281364
[synthesis and properties of digitoxigenin-3 beta-o-alpha-l-arabinofuranoside].	the synthesis of a cardenolide glycoside with a furanoide sugar component, digitoxigenin-3 beta-o-alpha-l-arabinofuranoside (4), is described for the first time. 4 was prepared by the reaction of digitoxigenin with 2,3,5-tri-o-benzoyl-alpha-l-arabinofuranosylchloride and fétizon-reagent in benzene/dioxan followed by debenzoylation with ammonia in dry methanol. compound 4 is cleaved by alpha-l-arabinofuranosidase (aspergillus niger k1) into digitoxigenin and l-arabinose. hydrolytic stability agai ...	1982	6298840
a physical map of the ribosomal dna repeat unit of aspergillus nidulans.	the ribosomal dna repeat unit of aspergillus nidulans has been cloned in pbr322 and a restriction map constructed. the genes coding for the 17s, 5.8s and 25s rrnas are found in blocks separated by a 1.7 kb spacer region, with the 5.8s rna gene lying between the genes for the two larger rnas. the total length of the repeat unit is 7.7 kb. the 5s rrna is not present in the repeat unit.	1982	6299883
structural characterization of neutral oligosaccharides of human midcycle cervical mucin.	it was previously shown that alkaline borohydride treatment of human midcycle cervical mucin releases a heterogeneous population of reduced neutral, sialylated, and sulfated oligosaccharides (yurewicz, e. c., and moghissi, k. s. (1981) j. biol. chem. 256, 11895-11905). three major neutral oligosaccharides were isolated with approximate compositions of fuc:gal:glcnac:n-acetylgalactosaminitol (galnacol) = 0:2:1:1 (a1), 1:2:1:1 (a2), and 2:2:1:1 (a3). they comprised roughly 21%, 13%, and 8% of huma ...	1982	6277894
cell wall degradation in the autolysis of filamentous fungi.	a systematic study on autolysis of the cell walls of fungi has been made on neurospora crassa, botrytis cinerea, polystictus versicolor, aspergillus nidulans, schizophyllum commune, aspergillus niger, and mucor mucedo. during autolysis each fungus produces the necessary lytic enzymes for its autodegradation. from autolyzed cultures of each fungus enzymatic precipitates were obtained. the degree of lysis of the cell walls, obtained from non-autolyzed mycelia, was studied by incubating these cell ...	1982	6219290
[aspergilli and chronic lung diseases].	in this study, mycological examinations of morning sputum of patients with chronic bronchitis and bronchial asthma has been performed. specimens were also examined from patients with lobar pneumonia as a control. a fumigatus and a. niger has been isolated from patients chronic bronchitis in frequency of 4.5% and 0.8% respectively. these 2 fungi has also been isolated from patients with bronchial asthma in frequency of 24.5% (a. fumigatus) and 2.4% (a. niger). the frequency of isolation of a. fum ...	1982	6755197
laser microbeam studies on fungal tissues.		1982	6757676
studies on air-borne fungi at qena. iii. thermophilic fungi.		1982	6759940
[microbiological test for the determination of the risk of spoilage of solid drug forms by the growth of molds].		1982	6760621
mycobacterial antibodies after tuberculin testing, bcg- vaccination, bcg-immunotherapy and against cross-reacting antigens in a solid-phase radioimmunoassay.	a solid-phase radioimmunoassay (spria) was used to investigate several factors influencing false positive and negative results in serodiagnostic test for tuberculosis, unheated cytoplasmic fractions of mycobacteria, and other microorganisms were bound to polystyrol and antibody binding of sera from tuberculous, non tuberculous hospitalized patients and healthy adults was tested by the spria-method. the level of antibody concentration in the spria reflects roughly cross-reactivity between the ant ...	1982	6805156
antimicrobial activity of carbazole derivatives.		1982	7047201
some comparative studies of two alkaline phosphatases from aspergillus niger.		1982	7056363
contribution to the fungal flora of cereal grains in egypt.	30 genera and 77 species, in addition to 4 varieties were isolated from 25 samples of each of barley, wheat, maize and sorghum grains collected from different places in egypt. the broadest spectrum of genera and species was recorded in wheat (25 genera and 59 species + 4 varieties) followed by barley (21 genera and 52 species + 2 varieties), sorghum (14 genera and 33 species + 2 varieties) and maize grains (11 genera and 29 species + 2 varieties). aspergillus, fusarium, penicillium, and rhizopus ...	1982	7070479
[isolation and identification of various bitter peptides from erythrocyte concentrates].		1982	6814109
[purification of exo-1,4-beta-xylosidase from aspergillus niger 15].	from the preparation of xylanase of the fungus aspergillus niger 15 exo-1,4-beta-xylosidase was isolated by means of ethanol fractionation and chromatography on columns of sephadex g-50, cellulose de-52, sephadex sp c-50, sephadex g-200. the isolated enzyme (with the purification degree as calculated per protein 199, and yield with respect to activity 42.5%) was homogeneous during gel filtration, polyacrylamide gel electrophoresis in the presence and absence of na dodecyl sulfate, ultracentrifug ...	1982	6815635
serodiagnostic value of culture filtrate antigens from aspergilli with septate phialides.	culture filtrate antigens from 6 typical isolates of aspergillus fumigatus, 6 isolates with septate phialides, and isolates of a. fumigatus var. ellipticus, a. fishceri, a. flavus and a. niger were studied in tests with 2,100 samples of serum originating from 1,452 patients suffering from miscellaneous diseases, including 60 cases of aspergillosis, 850 samples of serum from apparently healthy blood donors and 522 sera referred to us as positive for c-reactive protein. the culture filtrate antige ...	1982	6801788
action of glucoamylase from aspergillus niger on phosphorylated substrate.	glucoamylase (1,4-alpha-d-glucan glucohydrolase, ec 3.2.1.3) from aspergillus niger was purified to be free from alpha-amylase and phosphatase (glucose 6-phosphate as substrate). the phosphatase was well separated from the glucoamylase by phosphocellulose ion-exchange chromatography. the glucoamylase action was prevented by the esterified phosphate groups of the substrate. thus, the extensive action of the glucoamylase on potato starch exposed the 6-posphorylglucosyl residue of the starch at the ...	1982	6803838
structural similarities between a membrane-like surface layer on the cell wall of some microbial cells and the lamellar layer of vesicles formed by short-chain phosphonic acids in water. a freeze-fracture study.	a thin layer on the cell wall of some microbial cells, among them such of medical importance (aspergillus niger, trichophyton sp.), is characterized by a mosaic-like rodlet pattern in freeze-fracture electron microscopy. a very similar texture could be found in freeze-fractured bilayer vesicles of n-octan phosphonic acid in presence of 50% glycerol. the formation of this structure in phosphonic acid vesicles is connected with the transformation of the bilayer into the solid crystalline state by ...	1982	6807710
hydrophobic-ionic chromatography: its application to microbial glucose oxidase, hyaluronidase, cholesterol oxidase, and cholesterol esterase.	glucose oxidase from aspergillus niger, hyaluronidase from streptomyces hyalurolyticus, and cholesterol oxidase and cholesterol esterase from pseudomonas fluorescens were effectively adsorbed on an amberlite cg-50 column, when the cell-free cultured medium or the cultured medium with cell extract and without cell debris was applied without desalting but at ph less than or equal to 4.5. at the acidic ph, all the ion-exchange groups (-cooh) exist in the protonated form; the adsorption is not due t ...	1982	6807971
serology of aspergillus granuloma.	a total of 21 cases of granuloma caused by aspergillus species were encountered during the period 1972-79. the organs involved were nasal and paranasal sinuses, brain, orbit, subcutaneous tissue of check, lungs and endocardial valve in the decreasing order of their frequency. aspergillus flavus was the main etiological agent. immunodiffusion tests with various aspergillus species as antigen showed a positively of 17 out of 18 (94.4 per cent) cases. the role of precipitins in diagnosis and progno ...	1982	6813743
[the use of a glucoamylase enzyme preparation obtained from aspergillus niger 16/132, for the hydrolysis of corn starch].		1982	6816020
a simple method of affinity chromatography for the purification of glucoamylase obtained from aspergillus niger c.		1982	6818051
[the effects of various tetrahydro-1,3,5-thiadiazines as antifungal agents].		1982	6820681
aflatoxin and aflatoxicosis. i. fungal flora of some food and animal feeds with special references to aflatoxin-producing abilities.	a wide range of fungi, amounting to fifty-six species belonging to twenty-two genera, have been recovered from animal feeds and foodstuffs. the most frequent fungi were aspergillus niger, and a. flavus, followed by mucor racemosus, alternaria alternata. rhizopus stolonifer, penicillium corylophilum and p. notatum. three genera were found to be of moderate occurrence, namely, mucor, rhizopus and alternaria. the three following genera were of low occurrence: cladosporium, fusarium, and neurospora. ...	1982	7070483
frequency of clinical isolation and winter prevalence of different aspergillus species at a large southwestern army medical center.	the frequent occurrence of aspergillosis caused by species other than aspergillus fumigatus at brooke army medical center prompted the authors to study the prevalence of the different aspergillus species at brooke army medical center and at two other hospitals in san antonio, texas. two parameters were analyzed: (1) frequency of isolation from clinical specimens over a five-year period and (2) relative prevalence in the atmosphere during the late fall and early winter using volumetric air-sampli ...	1982	7073023
reaction pathways of substrate degradation by an acidic endo-1,4-beta-xylanase of aspergillus niger.	an acidic endo-1,4-beta-xylanase (1,4-beta-d-xylan xylanohydrolase, ec 3.2.1.8) of aspergillus niger catalyzes degradation of linear 1,4-beta-xylooligosaccharides by multiple reaction pathways analogous to those catalyzed by lysozyme and alpha-amylases. quantitative product analysis of enzyme-substrate mixtures using 1-3h-reducing end-labeled xylooligosaccharides and [u-14c]xylotriose led to the following conclusions: (1) bond cleavage frequencies of xylotriose, xylotetraose and xylopentaose are ...	1982	7093285
degradation of benzoate & salicylate by aspergillus niger.		1982	7106859
cellulase enzyme from aspergillus niger.	cellulase enzyme was produced by a selected strain of aspergillus niger isolated from deteriorated wood and grown on different carbon sources. filter paper gave the highest yield, followed by carboxymethyl cellulose (cmc). cellobiose as well as glucose gave a low yield, while the yield from lactose was negligible. the concentration of filter paper cellulose that induced the maximum yield of the enzyme was 1%. both soluble cellulose (cmc) and cotton cellulose treated with phosphoric acid (swollen ...	1982	7132786
effect of changed cultivation conditions on the morphology of aspergillus niger and on citric acid biosynthesis in laboratory cultivation.		1982	7141333
effect of aeration and agitation on the biosynthetic activity of diffusely growing aspergillus niger.	a considerable lowering of aeration demands occurs during diffuse growth of citric acid-producing aspergillus niger in a submerged cultivation. however, the diffuse culture poses stricter demands on the type of aeration and agitation. the impeller frequency affects considerably the morphology of the producer fungus and the accumulation of citric acid. the effect of impeller frequency on the distribution of air in the medium and on the amount of air fed into the diffuse culture is less important.	1982	7141334
aspergillus niger van tieghem mannosylation: polyprenylphosphate mannosyltransferase specificity.	aspergillus niger van tieghem microsomes contain an enzyme that catalyzes mannose transfer from gdp-mannose to polyprenylphosphate. the studies of the specificity of this enzyme for both the sugar donor (nucleoside diphosphate sugar) and the acceptor (polyprenylphosphates that were made available to the enzyme by means of the fusion of acceptor-loaded liposomes with the microsomal membranes) gave the following results. i) all the polyprenylphosphates from c15 to c120 were acceptors except retiny ...	1982	7142813
properties of aspergillus niger catalase.	catalase from aspergillus niger was purified to homogeneity as judged from the results of ultracentrifugation and polyacrylamide gel electrophoresis. the enzyme had a molecular weight of 385,000 as estimated from sedimentation measurements. carbohydrate analyses showed that the catalase was a glycoprotein containing about 8.3% neutral sugar and 1.9% glucosamine. under denaturing conditions, polyacrylamide gel electrophoresis revealed only one band with a molecular weight of 97,000 daltons in gel ...	1982	7153210
[use of a strain of aspergillus niger for purification of a hederagenin rhamnoglucoside from tubers of ficaria(ficaria ranunculoides moench)].		1982	7158960
[hydrazide derivatives of fluorobenzoic acids and their antimicrobial activity].	the compounds of formula (a) were prepared by reaction between rn-substituted benzoyl hydrazides and r-substituted benzoyl chlorides; the compounds of formula (b) were obtained by condensing r-substituted benzoyl chlorides with isonicotinic hydrazide. all the compounds were tested for in vitro activity against five gram+ bacteria (s. aureus, s. epidermidis, b. subtilis, b. anthracis, m. paratuberculosis atcc 607), seven gram-bacteria (s. paratyphi a, s. paratyphi b, e. coli, b. abortus, b. melit ...	1982	7168787
effect of vitamins on microbial production of citric acid by aspergillus niger.	the effect of different concentrations of ascorbic acid, biotin, p-aminobenzoic acid (paba), riboflavin and thiamine on the growth and activity of aspergillus niger strain al 29 was studied at different incubation periods. it was observed that ascorbic acid and paba inhibited the growth and activity of the mould, while the presence of thiamine and riboflavin stimulated the citric acid formation to the extent of 59.09% and 50.36%, respectively. however, biotin was found to be most suitable for in ...	1982	6983793
[leaching of uranium containing phosphorites with heterotrophic microorganisms].	the problem of heterotrophic leaching of metals was tested with uranium-containing phosphorites under laboratory conditions with regard to possible technical applications. as leaching agents we used the acids of citric and lactic acid fermentation, carried out with a strain of aspergillus niger in sulfite liquor under different conditions and with lactic acid bacteria in wheye. up to 12% uranium were soluted with citric acid fermentation of marocco phosphorite containing 153 ppm u in aspergillus ...	1982	7072261
[localised aspergillosis of the lung caused by aspergillus niger].	a 51-year old man with a history of pulmonary tuberculosis suffered an exacerbation without demonstration of tubercle bacilli. the mycological examination of sputum specimens discovered an infection with aspergillus niger. diagnosis was confirmed by the pure culture of this fungus in bronchial mucus and the cultural and histological identification in surgical material. as a histological feature strong double-refractile crystalline material was found in fungi and surrounding lung tissue (wall of ...	1982	7184222
the kinetic of lactose hydrolysis for the beta-galactosidase from aspergillus niger.	the kinetics of glucose liberation from lactose by means of the beta-glactosidase from aspergillus niger has been studied in a wide range of the main variables. the analysis shows that the kinetic models proposed so far are not adequate. the main finding is that the reaction rate is not linearly correlated to the enzyme concentration-it increase more than proportionally. this nonlinear relationship results because this lactase can distinguish between alpha-and beta-galactose alpha-galactose acts ...	1982	18546219
immobilization of mold and bacterial amylases on silica carriers.	the immobilization of alpha-amylase and glucoamylase was investigated by several coupling methods on silica carriers, different types of silokhroms, and silica gels. the most active immobilized mold and bacterial alpha-amylases and mold glucoamylase were obtained with titanium salts. these activities were twice the value of that obtained by glutaraldehyde or azo coupling. the half-lives of a. oryzae alpha-amylase, b. subtilis alpha-amylase, and a. niger glucoamylase, immobilized to silica carrie ...	1982	18548434
citric acid production with alginate bead entrappedaspergillus niger atcc 9142.	aspergillus niger atcc 9142 mycelium was entrapped in calcium alginate beads and employed in an air-lift completely stirred reactor for continuous production of citric acid. maximum yield obtained from 10% (w/v) sucrose was 12 g dm(-3) with about 40% fermentation efficiency. maximum rate of production 70 mg g(-1) h(-1) was about five times that obtained in classical batch fermentation.	1982	24234086
enzymic saccharification of sugarcane bagasse pretreated by autohydrolysis-steam explosion.	pretreatment of bagasse by autohydrolysis at 200 degrees c for 4 min and explosive defibration resulted in the solubilization of 90% of the hemicellulose (a heteroxylan) and in the production of a pulp that was highly susceptible to hydrolysis by cellulases from trichoderma reesei c-30 and qm 9414, and by a comercial preparation, meicelase. saccharification yields of 50% resulted after 24 h at 50 degrees c (ph 5.0) in enzymic digests containing 10% (w/v) bagasse pulps and 20 filter paper cellula ...	1983	18548636
catalytic properties of immobilized crude and pure glucoamylase from aspergillus niger c.		1983	18548651
effect of chemical modification of cellulose on the activity of a cellulase from aspergillus niger.	five chemically modified forms of cellulose were prepared, characterized, and tested as substrates for a homogeneous glucanohydrolase from a. niger. the relative order of reactivity at ph 4.0 was deae = pei > benzyl deae > cellulose > p > cm.the following abbreviations are used throughout the article: (rbb) remazol brilliant blue r; (deae) diethylamino ethyl; (pei) polethyleneimine; (cm) carboxymenthyl; (p) phospho; (ds) degree of rbb dye substitution of cellulose, in mol dye/100 glucose. this i ...	1983	18548761
aspergillus colonization associated with bilateral silicone mammary implants.	a case of aspergillus niger fungal colonization associated with bilateral inflatable silicone mammary implants is reported. painful fibrous capsular contractures without clinical evidence of infection or inflammation characterized the presenting symptoms. operative findings included a cheesy-white exudate that surrounded the implants and turbid fluid within the implants. all specimens yielded a heavy growth of aspergillus niger. special stains of the fibrous capsules were negative for fungal inv ...	1983	6823489
the active site of an acidic endo-1,4-beta-xylanase of aspergillus niger.	the substrate binding site of an acidic endo-1,4-beta-xylanase (1,4-beta-d-xylan xylanohydrolase, ec 3.2.1.8) of aspergillus niger was investigated using 1,4-beta-xylooligosaccharides (1-3h)-labelled at the reducing end. bond cleavage frequencies and v/km parameters of the oligosaccharides were determined under conditions of unimolecular hydrolysis and, according to the method of suganuma et al. (j. biochem. (tokyo) (1978) 84, 293-316), used for evaluation of subsite affinities. the substrate bi ...	1983	6824697
metabolism of resorcinol and salicylate in aspergillus niger.		1983	6825782
antimicrobial activity of 3-methyl-5-furfurylidene-2-thioxo-4-thiazolidones.	substituted 3-methyl-5-furfurylidene-2-thioxo-4-thiazolidones 1-17 were tested for their action against bacillus pumilus, b. brevis and b. megaterium and against aspergillus flavus, a. fumigatus and a. niger. most of the compounds possessed an activity against both bacteria and fungi. these compounds were also tested for their effectiveness as virus inhibitors employing nicotiana glutinosa as assay host against tobacco mosaic virus. most of them exhibited potential antiviral activity both in vit ...	1983	6832657
marijuana smoking and fungal sensitization.	the possible role of marijuana (mj) in inducing sensitization to aspergillus organisms was studied in 28 mj smokers by evaluating their clinical status and immune responses to microorganisms isolated from mj. the spectrum of illnesses included one patient with systemic aspergillosis and seven patients with a history of bronchospasm after the smoking of mj. twenty-one smokers were asymptomatic. fungi were identified in 13 of 14 mj samples and included aspergillus fumigatus, a. flavus, a. niger, m ...	1983	6833678
colorimetric immunoassays using flavin adenine dinucleotide as label.		1983	6855621
a new polyene with antimycotic activity produced by a streptomycete from the rhizosphere of an indoor plant.	from the rhizosphere of a potted sanseviera trifasciata, a streptomyces griseus strain with a broad antifungal activity was isolated; in the soil of this plant, a sole hyphomycete, aspergillus niger, was found in pure culture at 37 degrees c. this streptomycete produced a polyene, belonging to the pentaene group. it was found to inhibit, in vitro, the growth of all the 123 strains tested; the strains belonging to the genera aspergillus, cryptococcus, candida, trichophyton, epidermophyton, micros ...	1983	6681974
cyclosporin a inhibits coccidioides immitis in vitro and in vivo.	balb/c mice infected intraperitoneally with coccidioides immitis were treated with cyclosporin (cya) subcutaneously. cya prevented infection when treatment was started at day zero. when treatment was delayed until day 6 after infection, the mice that received either 75 or 25 mg/kg per day survived, but those treated with 7.5 mg/kg per day had the same mortality rate as controls. the higher doses of cya prevented dissemination of the fungus from the peritoneum to the lung but did not eliminate th ...	1983	6660859