Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| lipopolysaccharide (lps) stimulation of fungal secondary metabolism. | we report on a preliminary investigation of the use the gram-negative bacterial cell wall constituent lipopolysaccharide (lps) as a natural chemical cue to stimulate and alter the expression of fungal secondary metabolism. integrated high-throughput micro-cultivation and micro-analysis methods determined that 6 of 40 (15%) of fungi tested responded to an optimal exposure to lps (0.6 ng/ml) by activating, enhancing or accelerating secondary metabolite production. to explore the possible mechanism ... | 2014 | 25379339 |
| aspergillus fumigatus and related species. | the genus aspergillus contains etiologic agents of aspergillosis. the clinical manifestations of the disease range from allergic reaction to invasive pulmonary infection. among the pathogenic aspergilli, aspergillus fumigatus is most ubiquitous in the environment and is the major cause of the disease, followed by aspergillus flavus, aspergillus niger, aspergillus terreus, aspergillus nidulans, and several species in the section fumigati that morphologically resemble a. fumigatus. patients that a ... | 2014 | 25377144 |
| crystallization and preliminary x-ray diffraction data of β-galactosidase from aspergillus niger. | β-galactosidase from aspergillus niger (an-β-gal), belonging to the family 35 glycoside hydrolases, hydrolyzes the β-galactosidase linkages in lactose and other galactosides. it is extensively used in industry owing to its high hydrolytic activity and safety. the enzyme has been expressed in yeasts and purified by immobilized metal-ion affinity chromatography for crystallization experiments. the recombinant an-β-gal, deglycosylated to avoid heterogeneity of the sample, has a molecular mass of 10 ... | 2014 | 25372823 |
| purification, crystallization and preliminary x-ray diffraction analysis of nadp-dependent glutamate dehydrogenase from aspergillus niger. | glutamate dehydrogenase (gdh) catalyzes the nad-dependent or nadp-dependent oxidative deamination of l-glutamate to 2-oxoglutarate and ammonia. this important reversible reaction establishes the link between carbon and nitrogen metabolism. in this study, aspergillus niger nadp-gdh (angdh) has been overexpressed and purified. purified angdh, with a high specific activity of 631.1 units per milligram of protein, was crystallized and the crystal diffracted to 2.9 å resolution using a home x-ray sou ... | 2014 | 25372818 |
| flug affects secretion in colonies of aspergillus niger. | colonies of aspergillus niger are characterized by zonal heterogeneity in growth, sporulation, gene expression and secretion. for instance, the glucoamylase gene glaa is more highly expressed at the periphery of colonies when compared to the center. as a consequence, its encoded protein glaa is mainly secreted at the outer part of the colony. here, multiple copies of amyr were introduced in a. niger. most transformants over-expressing this regulatory gene of amylolytic genes still displayed hete ... | 2015 | 25370014 |
| morphological transitions under oxidative stress in response to metabolite formation in aspergillus niger. | oxidative stress is associated with metabolite formation in fungi. in contrast to an aspergillus niger wild-type strain, a sclerotia-formation regulator ansclr deletion strain demonstrated increased susceptibility to oxidative stress and reduced transcription of the catalase gene, catb, while an ansclr overexpression strain showed enhanced resistance to oxidative stress and increased expression of catb. in addition, ansclr complementation strain expressed a wild-type level of catb. the ansclr ov ... | 2015 | 25367801 |
| a mutation of aspergillus niger for hyper-production of citric acid from corn meal hydrolysate in a bioreactor. | the properties of the screened mutants for hyper-production of citric acid induced by carbon ((12)c(6+)) ion beams and x-ray irradiation were investigated in our current study. among these mutants, mutant h4002 screened from (12)c(6+) ion irradiation had a higher yield of citric acid production than the parental strain in a 250-ml shaking flash. these expanded submerged experiments in a bioreactor were also carried out for mutant h4002. the results showed that (177.7-196.0) g/l citric acid was a ... | 2014 | 25367793 |
| vea of aspergillus niger increases spore dispersing capacity by impacting conidiophore architecture. | aspergillus species are highly abundant fungi worldwide. their conidia are among the most dominant fungal spores in the air. conidia are formed in chains on the vesicle of the asexual reproductive structure called the conidiophore. here, it is shown that the velvet protein vea of aspergillus niger maximizes the diameter of the vesicle and the spore chain length. the length and width of the conidiophore stalk and vesicle were reduced nearly twofold in a δvea strain. the latter implies a fourfold ... | 2015 | 25367340 |
| effect of temperature, water activity, and ph on growth and production of ochratoxin a by aspergillus niger and aspergillus carbonarius from brazilian grapes. | the growth of ochratoxigenic fungus and the presence of ochratoxin a (ota) in grapes and their derivatives can be caused by a wide range of physical, chemical, and biological factors. the determination of interactions between these factors and fungal species from different climatic regions is important in designing models for minimizing the risk of ota in wine and grape juice. this study evaluated the influence of temperature, water activity (aw), and ph on the development and production of ota ... | 2014 | 25364929 |
| anti-oxidant studies and anti-microbial effect of origanum vulgare linn in combination with standard antibiotics. | origanum is one of the over 200 genera in the lamiaceae (mint family), and this genus includes culinary, fragrant, and medicinal properties. the plant is reported to contain anti-microbial properties, but it lacks combination studies with that of synthetic antibiotics. | 2014 | 25364204 |
| [determination of the antimicrobial capacity of green tea (camellia sinensis) against the potentially pathogenic microorganisms escherichia coli, salmonella enterica, staphylococcus aureus, listeria monocytogenes, candida albicans and aspergillus niger]. | many studies can be found in scientific literature demonstrating the antimicrobial capacity of different herbs, including green tea. never-theless, many results are divergent or cannot be compared. several green tea formulations may be found in market, but there is scarce or non-information about its activity. in this work, the potential antimicrobial effect of 50 samples of dry green tea and in 10% infusion against escherichia coli, salmonella enterica, listeria monocytogenes, staphylococcus au ... | 2013 | 25362825 |
| in-vitro antimicrobial, antibiofilm, cytotoxic, antifeedant and larvicidal properties of novel quinone isolated from aegle marmelos (linn.) correa. | plant metabolites have wide applications and have the potential to cure different diseases caused by microorganisms. the aim of the study was to evaluate the antimicrobial, antibiofilm, cytotoxic, antifeedant and larvicidal properties of novel quinine isolated from aegle marmelos (linn.) correa. | 2014 | 25359605 |
| preservative activity of lavender hydrosols in moisturizing body gels. | the study was undertaken to verify the antimicrobial activity of lavandula angustifolia hydrosols in moisturizing body gels. the inhibition efficacy of four lavender hydrosols (obtained from fresh or dry herbs or flowers) was tested against staphylococcus aureus atcc 6538, escherichia coli atcc 1627, candida sp. łock 0008 and aspergillus niger atcc 16404 in compliance with the standards of the european pharmacopoeia commission. although the tested hydrosols did not express any remarkable antimic ... | 2015 | 25353363 |
| fungi isolated from the excreta of wild birds in screening centers in pelotas, rs, brazil. | the identification of the fungal species belonging to the healthy microflora in animals is a precondition for the recognition of pathological processes causing them. the aim of this study was to investigate the presence of potentially pathogenic fungi in the feces of wild birds collected in screening centers. samples were collected from the feces of 50 cages with different species of birds. the samples were processed according to the modified method staib and the plates incubated at 32 °c for up ... | 2017 | 25351548 |
| mutualistic interaction between salmonella enterica and aspergillus niger and its effects on zea mays colonization. | salmonella typhimurium inhabits a variety of environments and is able to infect a broad range of hosts. throughout its life cycle, some hosts can act as intermediates in the path to the infection of others. aspergillus niger is a ubiquitous fungus that can often be found in soil or associated to plants and microbial consortia. recently, s. typhimurium was shown to establish biofilms on the hyphae of a. niger. in this work, we have found that this interaction is stable for weeks without a noticea ... | 2014 | 25351041 |
| analysis of metal bioleaching from thermal power plant fly ash by aspergillus niger 34770 culture supernatant and reduction of phytotoxicity during the process. | aspergillus niger culture supernatant is used for bioleaching process. before starting bioleaching process, fly ash was washed with distilled water. this removed 100 % sodium, 47 % (±0.45) boron, 38.07 % (±0.12) calcium, 29.89 % (±0.78) magnesium, and 11.8 % (±0.05) potassium. the ph was reduced from 10.5 to 8.5 after water washing. during bioleaching process, around 100 % metal removal was achieved in 4 h for all metals except chromium 93 % (±1.18), nickel 83 % (±0.32), arsenic 78 % (±0.52), an ... | 2015 | 25349087 |
| possible mechanism of antifungal phenazine-1-carboxamide from pseudomonas sp. against dimorphic fungi benjaminiella poitrasii and human pathogen candida albicans. | investigation of antifungal mechanism of phenazine 1-carboxamide (pc) produced by a pseudomonas strain mcc2142. | 2015 | 25348290 |
| bladder aspergillosis detected by urine cytology. | bladder aspergillosis is an unusual infection. we report the case of a 79-year-old man with clinical records of transitional cell carcinoma diagnosed 5 years ago. the presence of a fruiting body and septate hyphae in urine cytological smears were the key for a final diagnosis of fungal bladder infection caused by aspergillus niger. | 2015 | 25346364 |
| [cloning of feruloyl esterase gene from aspergillus niger h408 and high-efficient expression in pichia pastoris]. | to achieve the high-efficiency expression of feruloyl estrase gene (anfaea) from aspergillus niger h408 in pichia pastoris and characterize the recombinant feruloyl esterase (fae). | 2014 | 25345018 |
| effect of an organophosphate pesticide, monocrotophos, on phosphate-solubilizing efficiency of soil fungal isolates. | soil is a sink of pesticide residues as well as microorganisms. fungi are well known for solubilization of inorganic phosphates, and this activity of fungal isolates may be affected by the presence of pesticide residues in the soil. in the present study, five generically different fungal isolates, viz. aspergillus niger jq660373, aspergillus flavus, penicillium aculeatum jq660374, fusarium pallidoroseum and macrophomina sp., were tested and compared for their phosphate-solubilizing ability in th ... | 2015 | 25344433 |
| effect of carum copticum essential oil on growth and aflatoxin formation by aspergillus strains. | the objectives of this study were to determine the antiaflatoxin b1 activity in vitro of the essential oil (eo) extracted from the seeds of carum copticum and to evaluate its antifungal activity in vivo as a potential food preservative. the c. copticum eo exhibited noticeable inhibition on dry mycelium and synthesis of aflatoxin b1 (afb1) by aspergillus flavus, completely inhibiting afb1 production at 4 μl/ml. c. copticum eos showed the lowest percentages of decayed cherry tomatoes for all fungi ... | 2015 | 25342249 |
| an acidothermophilic functionally active novel gh12 family endoglucanase from aspergillus niger ho: purification, characterization and molecular interaction studies. | endoglucanase (eg) from aspergillus niger ho was sequentially purified through ultrafiltration, ion exchange and size exclusion chromatography to homogeneity, with an overall recovery of 18 %. the purified eg was a monomeric protein with a molecular weight of about 55 kda. the enzyme was optimally active at ph 3.5 and 70 °c with a half life (t1/2) of 3 h and km value of 2.5 mg/ml. metal ions, such as ca(2+) and co(2+) helped in enzyme induction, while hg(2+) and cu(2+) strongly inhibited the enz ... | 2015 | 25331339 |
| a tyrosinase inhibitor from aspergillus niger. | tyrosinase, in the presence of oxygen, is the main culprit in post harvest browning of food products, resulting in the drop in its commercial value. in an effort to seek natural tyrosinase inhibitors for food applications, a screening programme was undertaken. of the 26 fungal cultures isolated from soil samples of agumbe forest, india, one isolate s16, identified as aspergillus niger, gave an inhibition of 84 % against the enzyme. the inhibitor was isolated by following an enzyme inhibition ass ... | 2014 | 25328242 |
| effect of aspergillus niger xylanase on dough characteristics and bread quality attributes. | the present study was conducted to investigate the impact of various treatments of xylanase produced by aspergillus niger applied in bread making processes like during tempering of wheat kernels and dough mixing on the dough quality characteristics i.e. dryness, stiffness, elasticity, extensibility, coherency and bread quality parameters i.e. volume, specific volume, density, moisture retention and sensory attributes. different doses (200, 400, 600, 800 and 1,000 iu) of purified enzyme were appl ... | 2014 | 25328183 |
| a case of primary cutaneous scar infection caused by aspergillus niger. | 2014 | 25324671 | |
| development and evaluation of 99mtc-tricarbonyl-caspofungin as potential diagnostic agent of fungal infections. | infections by candida spp and aspergillus spp are the most common causes of invasive fungal infections. the main diagnostic methods are blood culture, and antigen-based techniques, but they are still suboptimal, leading to delays in the initiation of therapies and resulting in high mortality rates despite the availability of several new antifungal agents. the aim of this work was the development, synthesis and evaluation of a potential radiopharmaceutical enable the rapid and accurate diagnosis ... | 2014 | 25314957 |
| biotic elicitor enhanced production of psoralen in suspension cultures of psoralea corylifolia l. | cell cultures of psoralea corylifolia l. were established from the leaf disk derived callus. the effect of different biotic elicitors prepared from the fungal extract (aspergillus niger and penicillium notatum), yeast extract and chitosan with different concentrations was studied. the increased synthesis of psoralen in 16-day old cell cultures under 16 h of light and 8 h of dark period was studied. elicitation of psoralen in a. niger elicitor treated cells was found 9-fold higher over control ce ... | 2014 | 25313287 |
| in vitro antioxidant activity and total phenolic content of endophytic fungi isolated from eugenia jambolana lam. | to elucidate the antioxidant activity and total phenolic content (tpc) of ethyl acetate extracts of endophytic fungi isolated from eugenia jambolana by three different antioxidant assays. | 2014 | 25312132 |
| screening and study of antifungal activity of leaf litter actinomycetes isolated from ternate island, indonesia. | to characterize abundance of leaf litter actinomycetes from ternate island and to assess the antifungal activity of actinomycetes isolates against candida albicans, saccharomyces cerevisiae (s. cerevisiae), and aspergillus niger. | 2014 | 25312129 |
| fluoride-tolerant mutants of aspergillus niger show enhanced phosphate solubilization capacity. | p-solubilizing microorganisms are a promising alternative for a sustainable use of p against a backdrop of depletion of high-grade rock phosphates (rps). nevertheless, toxic elements present in rps, such as fluorine, can negatively affect microbial solubilization. thus, this study aimed at selecting aspergillus niger mutants efficient at p solubilization in the presence of fluoride (f-). the mutants were obtained by exposition of conidia to uv light followed by screening in a medium supplemented ... | 2014 | 25310310 |
| production and immobilization of enzymes by solid-state fermentation of agroindustrial waste. | the recovery of by-products from agri-food industry is currently one of the major challenges of biotechnology. castilla-la mancha produces around three million tons of waste coming from olive oil and wine industries, both of which have a pivotal role in the economy of this region. for this reason, this study reports on the exploitation of grape skins and olive pomaces for the production of lignocellulosic enzymes, which are able to deconstruct the agroindustrial waste and, therefore, reuse them ... | 2015 | 25300855 |
| enhanced production of xylanase from locally isolated fungal strain using agro-industrial residues under solid-state fermentation. | this study is related to the isolation of fungal strain for xylanase production using agro-industrial residues. forty fungal strains with xylanolytic potential were isolated by using xylan agar plates and quantitatively screened in solid-state fermentation. of all the tested isolates, the strain showing highest ability to produce xylanase was assigned the code aspergillus niger lcbt-14. for the enhanced production of the enzyme, five different fermentation media were evaluated. out of all media, ... | 2015 | 25299357 |
| antimicrobial applications of transition metal complexes of benzothiazole based terpolymer: synthesis, characterization, and effect on bacterial and fungal strains. | terpolymer of 2-amino-6-nitro-benzothiazole-ethylenediamine-formaldehyde (bef) has been synthesized and characterized by elemental analysis and various spectral techniques like ftir, uv-visible, and (1)h and (13)c-nmr. the terpolymer metal complexes were prepared with cu(2+), ni(2+), and zn(2+) metal ions using bef terpolymer as a ligand. the complexes have been characterized by elemental analysis and ir, uv-visible, esr, (1)h-nmr, and (13)c-nmr spectral studies. gel permeation chromatography wa ... | 2014 | 25298760 |
| immobilization of cellulase on tio2 nanoparticles by physical and covalent methods: a comparative study. | immobilization of cellulase from aspergillus niger on tio2 nanoparticles was studied by two different approaches--physical adsorption and covalent coupling. a. niger was selected, as it is generally non-pathogenic, is found in nature in the broad range of habitats and produces cellulase extracellulary. for covalent method, tio2 nanoparticles were modified with aminopropyltriethoxysilane (apts). the adsorbed and covalently immobilized enzymes showed 76% and 93% activity, respectively, as compared ... | 2014 | 25296503 |
| characterization of four new antifungal yanuthones from aspergillus niger. | four new yanuthone analogs (1-4) were isolated from the filamentous fungus aspergillus niger. the structures of the new compounds were elucidated on the basis of uhplc-dad-hrms data and one-dimensional and two-dimensional nmr spectroscopy. labeling studies with (13)c8-6-methylsalicylic acid identified three class i yanuthones originating from the polyketide 6-methylsalicylic acid (yanuthone k, l and m (1-3)) and a class ii yanuthone, which was named yanuthone x2 (4). the four new compounds were ... | 2015 | 25293978 |
| screening the antifungal activity of essential oils against decay fungi from palmyrah leaf handicrafts. | the whitish tender leaves of palmyrah are used for making handicrafts. the problem with these articles is discolouration with time and become more brittle due to fungal attack. this could be prevented by some protective coating. instead of expensive and harmful chemicals we decided to test natural plant essential oils to control fungal attack. palmyrah leaf article decay fungi were isolated from two different sites of jaffna peninsula. in this investigation antifungal activity of different plant ... | 2014 | 25287894 |
| synthesis, in vitro anticancer and antimicrobial evaluation of novel substituted dihydropyrimidines. | a series of 1,4-dihydropyrimidine derivatives 3(a-t) were prepared from biginelli reactions by using ethyl acetoacetate, substituted benzaldehyde and thiourea in the presence of piperidine and ethanol. the compounds 3(a-t) were reacted with dimethylsulphate, diethylsulphate, butyl bromide and benzyl chloride to give the new series of compounds 4(a-t). the structures of the newly synthesized compounds 4(a-t) were established by ir, (1)h nmr, mass spectra and elemental analysis. the synthesized co ... | 2014 | 25284932 |
| variation in the fumonisin biosynthetic gene cluster in fumonisin-producing and nonproducing black aspergilli. | the ability to produce fumonisin mycotoxins varies among members of the black aspergilli. previously, analyses of selected genes in the fumonisin biosynthetic gene (fum) cluster in black aspergilli from california grapes indicated that fumonisin-nonproducing isolates of aspergillus welwitschiae lack six fum genes, but nonproducing isolates of aspergillus niger do not. in the current study, analyses of black aspergilli from grapes from the mediterranean basin indicate that the genomic context of ... | 2014 | 25281783 |
| in vitro pharmacological screening of three newly synthesised pyrimidine derivatives. | the antibacterial and antifungal activities of three new pyrimidine derivatives, namely, 2,6-bis(4,6-dimethylpyrimidin-2-ylthio)benzene-1,4-diol (1),3,5-bis(4,6-dimethylpyrimidin-2-ylthio)-2-methylbenzene-1,4-diol (2) and 3,5-bis(4,6-dimethylpyrimidin-2-ylthio)-2-methoxybenzene-1,4-diol (3), synthesised by electrochemical method are presented here. the compounds were screened for their activities against gram-positive and gram-negative bacteria, bacillus subtilis, staphylococcusaureus, escherich ... | 2015 | 25280048 |
| [inulinases from various producers: the features of their permolecular organization]. | the structural organization of inulinases from yeasts, fungi, and plants are researched. for studying their sizes, molecular weight, and permolecular organization, an approach consisting of a combination of atomic force microscopy with methods of dynamic light scattering, gel chromatography, and electrophoresis was used. it is shown that inulinases from kluyveromyces marxianus and aspergillus niger form geterodimers and inulinases from tubers of helianthus tuberosus are present as both dimers an ... | 2014 | 25272747 |
| comparison of five different c18 hplc analytical columns for the analysis of ochratoxin a in different matrices. | the performance of five different c18 chromatography analytical columns with different lengths, particle sizes and porosities were compared for analysis of ochratoxin a (ota) in fungal cultures and raisin samples. chromatographic parameters including retention time, limit of detection, limit of quantification, number of theoretical plates and reduced plate height were obtained and compared. this showed that, compared with traditional columns, shorter ones (100 and 75mm×4.6mm) with 2.7μm solid co ... | 2014 | 25270057 |
| liquefaction of sugarcane bagasse for enzyme production. | the objective of this paper is to report liquefaction of pretreated and sterilized sugarcane bagasse for enhancing endoglucanase production through submerged fermentation by aspergillus niger. after initial solid state fermentation of steam pretreated bagasse solids by a. niger, fed-batch addition of the substrate to cellulase in buffer over a 12h period, followed by 36h reaction, resulted in a liquid slurry with a viscosity of 0.30±0.07pas at 30% (w/v) solids. addition of a. niger for submerged ... | 2014 | 25265329 |
| efficient and cost-reduced glucoamylase fed-batch production with alternative carbon sources. | glucoamylase is an important industrial enzyme. glucoamylase production by industrial aspergillus niger strain featured with two major problems: (i) empirical substrate feeding methods deteriorating the fermentation performance; and (ii) the high raw materials cost limiting the economics of the glucoamylase product with delegated specification. in this study, we first proposed a novel three-stage varied-rate substrate feeding strategy for efficient glucoamylase production in a 5 l bioreactor usi ... | 2015 | 25262682 |
| overexpression, purification and characterisation of homologous α-l-arabinofuranosidase and endo-1,4-β-d-glucanase in aspergillus vadensis. | in the recent past, much research has been applied to the development of aspergillus, most notably a. niger and a. oryzae, as hosts for recombinant protein production. in this study, the potential of another species, aspergillus vadensis, was examined. the full length gdna encoding two plant biomass degrading enzymes, i.e. α-l-arabinofuranosidase (abfb) (gh54) and endo-1,4-β-d-glucanase (egla) (gh12) from a. vadensis were successfully expressed using the gpda promoter from a. vadensis. both enzy ... | 2014 | 25260653 |
| the characterization of the endoglucanase cel12a from gloeophyllum trabeum reveals an enzyme highly active on β-glucan. | the basidiomycete fungus gloeophyllum trabeum causes a typical brown rot and is known to use reactive oxygen species in the degradation of cellulose. the extracellular cel12a is one of the few endo-1,4-β-glucanase produced by g. trabeum. here we cloned cel12a and heterologously expressed it in aspergillus niger. the identity of the resulting recombinant protein was confirmed by mass spectrometry. we used the purified gtcel12a to determine its substrate specificity and basic biochemical propertie ... | 2014 | 25251390 |
| sorbicillinoid analogs with cytotoxic and selective anti-aspergillus activities from scytalidium album. | as part of an ongoing project to explore filamentous fungi for anticancer and antibiotic leads, 11 compounds were isolated and identified from an organic extract of the fungus scytalidium album (msx51631) using bioactivity-directed fractionation against human cancer cell lines. of these, eight compounds were a series of sorbicillinoid analogs (1-8), of which four were new (scalbucillin a (2), scalbucillin b (3), scalbucillin c (6) and scalbucillin d (8)), two were phthalides (9-10) and one was n ... | 2015 | 25248727 |
| dna cleavage, antibacterial, antifungal and anthelmintic studies of co(ii), ni(ii) and cu(ii) complexes of coumarin schiff bases: synthesis and spectral approach. | the metal complexes of co(ii), ni(ii) and cu(ii) have been synthesized from 6-formyl-7,8-dihydroxy-4-methylcoumarin with o-toluidine/3-aminobenzotrifluoride. the synthesized schiff bases and their metal complexes were structurally characterized based on ir, (1)h nmr, (13)c nmr, uv-visible, esr, magnetic, thermal, fluorescence, mass and esi-ms studies. the molar conductance values indicate that complexes are non-electrolytic in nature. elemental analysis reveals ml2·2h2o [m = co(ii), ni(ii) and c ... | 2015 | 25244297 |
| development of rflp-pcr method for the identification of medically important aspergillus species using single restriction enzyme mwoi. | in this study we attempted to modify the pcr-rflp method using restriction enzyme mwoi for the identification of medically important aspergillus species. our subjects included nine standard aspergillus species and 205 aspergillus isolates of approved hospital acquired infections and hospital indoor sources. first of all, aspergillus isolates were identified in the level of species by using morphologic method. a twenty four hours culture was performed for each isolates to harvest aspergillus myce ... | 2014 | 25242934 |
| utilization of molasses and sugar cane bagasse for production of fungal invertase in solid state fermentation using aspergillus niger gh1. | agro-industrial wastes have been used as substrate-support in solid state fermentation for enzyme production. molasses and sugarcane bagasse are by-products of sugar industry and can be employed as substrates for invertase production. invertase is an important enzyme for sweeteners development. in this study, a xerophilic fungus aspergillus niger gh1 isolated of the mexican semi-desert, previously reported as an invertase over-producer strain was used. molasses from mexico and cuba were chemical ... | 2014 | 25242918 |
| controlled expression of pectic enzymes in arabidopsis thaliana enhances biomass conversion without adverse effects on growth. | lignocellulosic biomass from agriculture wastes is a potential source of biofuel, but its use is currently limited by the recalcitrance of the plant cell wall to enzymatic digestion. modification of the wall structural components can be a viable strategy to overcome this bottleneck. we have previously shown that the expression of a fungal polygalacturonase (pga2 from aspergillus niger) in arabidopsis and tobacco plants reduces the levels of de-esterified homogalacturonan in the cell wall and sig ... | 2015 | 25242621 |
| lemon grass (cymbopogon citratus) essential oil as a potent anti-inflammatory and antifungal drugs. | volatile oils obtained from lemon grass [cymbopogon citratus (dc.) stapf, poaceae family] are used in traditional medicine as remedies for the treatment of various diseases. | 2014 | 25242268 |
| enhanced enzymatic hydrolysis of waste paper for ethanol production using separate saccharification and fermentation. | ethanol produced from lignocellulosic biomass is a renewable alternative to diminishing petroleum-based liquid fuels. in this study, the feasibility of ethanol production from waste paper using the separate hydrolysis and fermentation (shf) was investigated. two types of waste paper materials, newspaper and office paper, were evaluated for their potential to be used as a renewable feedstock for the production of fermentable sugars via enzymatic hydrolysis of their cellulose fractions. hydrolysis ... | 2015 | 25234398 |
| application of an online-biomass sensor in an optical multisensory platform prototype for growth monitoring of biotechnical relevant microorganism and cell lines in single-use shake flasks. | in the context of this work we evaluated a multisensory, noninvasive prototype platform for shake flask cultivations by monitoring three basic parameters (ph, po2 and biomass). the focus lies on the evaluation of the biomass sensor based on backward light scattering. the application spectrum was expanded to four new organisms in addition to e. coli k12 and s. cerevisiae [1]. it could be shown that the sensor is appropriate for a wide range of standard microorganisms, e.g., l. zeae, k. pastoris, ... | 2014 | 25232914 |
| overexpression of the nadp+-specific isocitrate dehydrogenase gene (icda) in citric acid-producing aspergillus niger wu-2223l. | in the tricarboxylic acid (tca) cycle, nadp(+)-specific isocitrate dehydrogenase (nadp(+)-icdh) catalyzes oxidative decarboxylation of isocitric acid to form α-ketoglutaric acid with nadp(+) as a cofactor. we constructed an nadp(+)-icdh gene (icda)-overexpressing strain (opi-1) using aspergillus niger wu-2223l as a host and examined the effects of increase in nadp(+)-icdh activity on citric acid production. under citric acid-producing conditions with glucose as the carbon source, the amounts of ... | 2014 | 25229866 |
| purification and characterization of three β-glycosidases exhibiting high glucose tolerance from aspergillus niger asku28. | production and utilization of cellulosic ethanol has been limited, partly due to the difficulty in degradation of cellulosic feedstock. β-glucosidases convert cellobiose to glucose in the final step of cellulose degradation, but they are inhibited by high concentrations of glucose. thus, in this study, we have screened, isolated, and characterized three β-glycosidases exhibiting highly glucose-tolerant property from aspergillus niger asku28, namely β-xylosidase (p1.1), β-glucosidase (p1.2), and ... | 2014 | 25229852 |
| carbon nanotubes as supports for inulinase immobilization. | the commercial inulinase obtained from aspergillus niger was non-covalently immobilized on multiwalled carbon nanotubes (mwnt-cooh). the immobilization conditions for the carbon nanotubes were defined by the central composite rotational design (ccrd). the effects of enzyme concentration (0.8%-1.7% v/v) and adsorbent:adsorbate ratio (1:460-1:175) on the enzyme immobilization were studied. the adsorbent:adsorbate ratio variable has positive effect and the enzyme concentration has a negative effect ... | 2014 | 25225722 |
| characterization of the starvation-induced chitinase cfca and α-1,3-glucanase agnb of aspergillus niger. | the common saprophyte aspergillus niger may experience carbon starvation in nature as well as during industrial fermentations. starvation survival strategies, such as conidiation or the formation of exploratory hyphae, require energy and building blocks, which may be supplied by autolysis. glycoside hydrolases are key effectors of autolytic degradation of fungal cell walls, but knowledge on their identity and functionality is still limited. we recently identified agnb and cfca as two genes encod ... | 2015 | 25219534 |
| nitric oxide mediates hypocrellin accumulation induced by fungal elicitor in submerged cultures of shiraia bambusicola. | multiple responses of shiraia bambusicola, including nitric oxide (no) generation, hypocrellins production and salicylic acid (sa) biosynthesis, were induced by a fungal elicitor prepared from the mycelium of aspergillum niger. both the no donator, sodium nitroprusside, and sa enhanced hypocrellin production without the fungal elicitor. however, the no scavenger, 2,4-carboxyphenyl-4,4, 5,5- tetramethylimidazoline-1-oxyl-3-oxide (cptio) and the sa biosynthesis inhibitor, cinnamic acid (ca), inhib ... | 2015 | 25214226 |
| diversity of soil fungi in north 24 parganas and their antagonistic potential against leucinodes orbonalis guen. (shoot and fruit borer of brinjal). | soil samples were collected from agricultural fields and gardens in north 24 parganas, west bengal, and fungi species were isolated from them. thirty-one fungal species were isolated with 19 found in agricultural soil and 28 in garden soil. twenty-eight out of 31 were identified using cultural and microscopic characters, and three were unidentified. the diversity of isolated fungi was calculated by simpson's diversity index. the garden soil possessed more fungal colonies (750) than agricultural ... | 2014 | 25213561 |
| evaluation of mycotoxins, mycobiota, and toxigenic fungi in selected medicinal plants of khyber pakhtunkhwa, pakistan. | medicinal plants are used worldwide to treat a variety of ailments. due to the provenance of medicinal plants, they are subjected to contamination by moulds, which may be responsible for spoilage and production of mycotoxins. the investigation was designed to throw light on mycological and mycotoxicological status of some medicinal plants from pakistan and the result showed 30 % and 26.7 % samples were contaminated with aflatoxins and ochratoxin a, respectively. mould contamination was present i ... | 2014 | 25209636 |
| efficiency of artemisia nilagirica (clarke) pamp. essential oil as a mycotoxicant against postharvest mycobiota of table grapes. | in order to get a potent botanical fungicide for the management of fungal decay of table grapes, an experiment was conducted in which 20 essential oils of higher plants were screened at 0.33 µl ml(-1) against dominant fungi causing decay of table grapes, including aspergillus flavus, a. niger and a. ochraceus. furthermore, the minimum inhibitory/fungicidal concentration, fungitoxic spectrum and mycotoxin inhibition activity of the most potent oil were determined. the efficacy of the most potent ... | 2015 | 25199920 |
| optimization of the integrated citric acid-methane fermentation process by air stripping and glucoamylase addition. | to solve the problem of extraction wastewater in citric acid industry, an integrated citric acid-methane fermentation process was proposed. in the integrated process, extraction wastewater was treated by mesophilic anaerobic digestion and then reused to make mash for the next batch of citric acid fermentation. in this study, an aspergillus niger mutant strain exhibiting resistance to high metal ions concentration was used to eliminate the inhibition of 200 mg/l na(+) and 300 mg/l k(+) in anaerob ... | 2015 | 25194466 |
| production of a bioflocculant from aspergillus niger using palm oil mill effluent as carbon source. | this study evaluated the potential of bioflocculant production from aspergillus niger using palm oil mill effluent (pome) as carbon source. the bioflocculant named pm-5 produced by a. niger showed a good flocculating capability and flocculating rate of 76.8% to kaolin suspension could be achieved at 60 h of culture time. glutamic acid was the most favorable nitrogen source for a. niger in bioflocculant production at ph 6 and temperature 35 °c. the chemical composition of purified pm-5 was mainly ... | 2014 | 25189510 |
| utilization of potato starch processing wastes to produce animal feed with high lysine content. | this work aims to utilize wastes from the potato starch industry to produce single-cell protein (scp) with high lysine content as animal feed. in this work, s-(2-aminoethyl)-l-cysteine hydrochloride-resistant bacillus pumilus e1 was used to produce scp with high lysine content, whereas aspergillus niger was used to degrade cellulose biomass and candida utilis was used to improve the smell and palatability of the feed. an orthogonal design was used to optimize the process of fermentation for maxi ... | 2015 | 25189407 |
| a high-throughput method for screening of aspergillus niger mutants with high transglycosylation activity by detecting non-fermentable reducing sugar. | a novel high-throughput method was established for rapid screening of large numbers of aspergillus niger mutants with high transglucosylation activity by exploiting that yeast can hardly hydrolyze isomaltooligosaccharides (imo). supernatants of a. niger fermentation were incubated with saccharomyces cerevisiae to remove glucose and maltose, and the remaining non-reducing sugars, which is positively correlated with the amount of imo, the products of transglucosylation reaction, were used as indic ... | 2011 | 25187152 |
| physico-chemical analysis and antimicrobial potential of apis dorsata, apis mellifera and ziziphus jujube honey samples from pakistan. | to evaluate physico-chemical properties and antimicrobial potential of indigenous honey samples against different reference strains including escherichia coli atcc 8739, enterobacter aerogenes atcc 13048, pseudomonas aeroginosa atcc 9027, bacillus subtilis atcc 6633, staphylococcus aureus atcc 25923, salmonella typhi atcc 14028, klebsiella pneumonia atcc 13883, aspergillus niger atcc 16404, rhizopus oligosporus pcsir1, candida albicans atcc 14053 and candida utilis atcc 9950. | 2014 | 25183333 |
| polyketide and benzopyran compounds of an endophytic fungus isolated from cinnamomum mollissimum: biological activity and structure. | to study bioactivity and compounds produced by an endophytic phoma sp. fungus isolated from the medicinal plant cinnamomum mollissimum. | 2014 | 25183332 |
| effect of essential oils on aspergillus spore germination, growth and mycotoxin production: a potential source of botanical food preservative. | to investigate effect of essential oils on aspergillus spore germination, growth and mycotoxin production. | 2014 | 25183114 |
| fungal type iii polyketide synthases. | this article covers the literature on fungal type iii polyketide synthases (pkss) published from 2005 to 2014. since the first discovery of fungal type iii pks genes in aspergillus oryzae, reported in 2005, putative genes for type iii pkss have been discovered in fungal genomes. compared with type i pkss, type iii pkss are much less abundant in fungi. however, type iii pkss could have some critical roles in fungi. this article summarizes the studies on fungal type iii pks functional analysis, in ... | 2014 | 25182423 |
| transformation of vanadinite [pb5 (vo4 )3 cl] by fungi. | saprotrophic fungi were investigated for their bioweathering effects on the vanadium- and lead-containing insoluble apatite group mineral, vanadinite [pb5 (vo4 )3 cl]. despite the insolubility of vanadinite, fungi exerted both biochemical and biophysical effects on the mineral including etching, penetration and formation of new biominerals. lead oxalate was precipitated by aspergillus niger during bioleaching of natural and synthetic vanadinite. some calcium oxalate monohydrate (whewellite) was ... | 2015 | 25181352 |
| the case of diagnostics of invasive pulmonary aspergillosis by in vivo probe-based confocal laser endomicroscopy of central and distal airways. | we present a case of 41-year-old patient with invasive pulmonary aspergillosis (ipa) in which probe-based confocal laser endomicroscopy (pcle) imaging of central and distal airways was first performed in vivo. pcle imaging showed the signs of complete or partial destruction of elastin network of alveolar wall with fibrillar branching fluorescent structures in the zone with typical ipa changes on hrct. | 2014 | 25180153 |
| effects of temperature and additives on the thermal stability of glucoamylase from aspergillus niger. | gam-1 and gam-2, two themostable glucoamylases from aspergillus niger b-30, possess different molecular masses, glycosylation, and thermal stability. in the present study, the effects of additives on the thermal inactivation of gam-1 and gam-2 were investigated. the half-lives of gam-1 and gam-2 at 70°c were 45 and 216 min, respectively. data obtained from fluorescence spectroscopy, circular dichroism spectroscopy, uv absorption spectroscopy, and dynamic light scattering demonstrated that during ... | 2015 | 25179903 |
| overexpression of the aspergillus niger gata transporter leads to preferential use of d-galacturonic acid over d-xylose. | pectin is a structural heteropolysaccharide of the primary cell walls of plants and as such is a significant fraction of agricultural waste residues that is currently insufficiently used. its main component, d-galacturonic acid, is an attractive substrate for bioconversion. the complete metabolic pathway is present in the genome of aspergillus niger, that is used in this study. the objective was to identify the d-galacturonic acid transporter in a. niger and to use this transporter to study d-ga ... | 2014 | 25177540 |
| synthesis aspects, structural, spectroscopic, antimicrobial and room temperature ferromagnetism of zinc iodide complex with schiff based ligand. | in this paper, we report the successful growth of complex compound of zinc iodide with thiocarbamide by slow evaporation method. the single crystal xrd study reveals that the crystal belongs to monoclinic system with centrosymmetric space group and powder xrd analysis shows that the perfect crystalline nature of the crystal. the presence of functional group and element were confirmed from ft-ir and edax analysis. optical absorbance of the grown crystal was studied by uv-vis spectrophotometer. th ... | 2015 | 25173522 |
| in vitro antimicrobial activity of pistacia lentiscus l. edible oil and phenolic extract. | pistacia lentiscus l. is known in some tunisian forest area by its fixed oil used in traditional medicine as an antiseptic product. this investigation is the first to study the antimicrobial activity of p.lentiscus edible oil and its phenolic extract. oil was extracted from fruits harvested from six provenances located in tunisia. the antimicrobial activity was tested using disc diffusion assay and the broth dilution method. kbouch and sidi zid oils were most efficient (p < 0.003) against, respe ... | 2015 | 25157979 |
| improvement of the optimum ph of aspergillus niger xylanase towards an alkaline ph by site-directed mutagenesis. | in an attempt to shift the optimal ph of the xylanase b (xynb) from aspergillus niger towards alkalinity, target mutation sites were selected by alignment between aspergillus niger xylanase b and other xylanases that have alkalophilic ph optima that highlight charged residues in the eight-residues-longer loop in the alkalophilic xylanase. multiple engineered xynb mutants were created by site-directed mutagenesis with substitutions q164k and q164k+d117n. the variant xynb-117 had the highest optim ... | 2015 | 25152057 |
| effect of the nematode biocide dbx-1003 in controlling citrus nematode infecting mandarin, and interrelationship with the co-inhabitant fungi. | in a field experiment, the nematode-biocide, dbx-1003, was evaluated against the citrus nematode, tylenchulus semipenetrans infecting mandarin, citrus reticulata, dbx-1003 20% g., was applied in october 2008 at the rate 1/2 kg/tree, and root and soil samples were collected monthly until the next october, 2009. successive treatment of the same biocide was added in may 2009. for the comparison, vydate 24% l, was applied as well as non-treated check trees were left. data revealed that the biocide t ... | 2013 | 25151818 |
| novel 1,5,7-trihydroxy-3-hydroxy methyl anthraquinone isolated from terrestrial streptomyces sp. (eri-26) with antimicrobial and molecular docking studies. | streptomyces sp. isolate eri-26 was obtained from the nilgiris forest soil of western ghats, tamil nadu, india. novel anthraquinone compound was isolated from the active fraction 5; it was identified by spectroscopical data using uv, ir, nmr and mass. the isolated compound 1,5,7-trihydroxy-3-hydroxy methyl anthraquinone was tested against bacteria and fungi at minimum inhibitory concentration level. the compound showed significant antimicrobial activity against bacteria, staphylococcus aureus at ... | 2014 | 25149455 |
| mold contamination of untreated and roasted with salt nuts (walnuts, peanuts and pistachios) sold at markets of tabriz, iran. | nuts are one of the main consumed snacks worldwide and a significant component of iranian's diet. natural contamination of nuts with fungus is unavoidable and is a major challenge to nuts safety and quality. | 2014 | 25147659 |
| antibacterial and antifungal activity of holothuria leucospilota isolated from persian gulf and oman sea. | emergence of antimicrobial resistance toward a number of conventional antibiotics has triggered the search for antimicrobial agents from a variety of sources including the marine environment. | 2014 | 25147657 |
| cryo-electron microscopy specimen preparation by means of a focused ion beam. | here we present a protocol used to prepare cryo-tem samples of aspergillus niger spores, but which can easily be adapted for any number of microorganisms or solutions. we make use of a custom built cryo-transfer station and a modified cryo-sem preparation chamber. the spores are taken from a culture, plunge-frozen in a liquid nitrogen slush and observed in the cryo-sem to select a region of interest. a thin lamella is then extracted using the fib, attached to a tem grid and subsequently thinned ... | 2014 | 25146386 |
| antimicrobial activity and agricultural properties of bitter melon (momordica charantia l.) grown in northern parts of turkey: a case study for adaptation. | this study was designed to determine the adaptation capability of bitter melon (momordica charantia l.), which is widely grown in tropical and subtropical climates, in northern parts of turkey. in this study, plant height, number of fruits, fruit length, fruit width, number of seeds and fruit weight of bitter melon grown in field conditions were determined. the antimicrobial effect of the ethanol extract of fruit and seeds against pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, ... | 2015 | 25141891 |
| eucast testing of isavuconazole susceptibility in aspergillus: comparison of results for inoculum standardization using conidium counting versus optical density. | the eucast e.def9.1 standard recommends standardization of the inoculum concentration by conidium counting using a hemocytometer rather than a spectrophotometer. in this study, we investigated whether the choice of these methods influenced isavuconazole mics. a blinded collection of 30 molecularly characterized azole-resistant isolates and 10 wild-type aspergillus fumigatus isolates was shared with four different laboratories. additionally, each laboratory selected approximately 100 a. fumigatus ... | 2014 | 25136005 |
| fungal diversity, incidence and mycotoxin contamination in grapes from two agro-climatic spanish regions with emphasis on aspergillus species. | fourteen vineyards from two different agro-climatic regions in spain were sampled in two consecutive years in order to determinate the grape mycobiota and diversity indexes with the final aim to define the potential mycotoxigenic species from both regions and their relationship. | 2015 | 25131651 |
| coprinopsis atramentaria extract, its organic acids, and synthesized glucuronated and methylated derivatives as antibacterial and antifungal agents. | despite the available data regarding antimicrobial activity of phenolic acids, studies dealing with the effects of their metabolites or derivatives are scarce. therefore, the antimicrobial and demelanizing activities of coprinopsis atramentaria extract, its organic acids, and methylated and glucuronated derivatives were evaluated. the antifungal activity was stronger than the antibacterial effects. in general, individual compounds (mostly organic acids) exhibited higher activity than the extract ... | 2014 | 25131614 |
| structural analysis of n- and o-glycans using zic-hilic/dialysis coupled to nmr detection. | protein glycosylation, an important and complex post-translational modification (ptm), is involved in various biological processes, including the receptor-ligand and cell-cell interaction, and plays a crucial role in many biological functions. however, little is known about the glycan structures of important biological complex samples, and the conventional glycan enrichment strategy (i.e., size-exclusion column [sec] separation) prior to nuclear magnetic resonance (nmr) detection is time-consumi ... | 2014 | 25117693 |
| synergistic effect of aspergillus niger and trichoderma reesei enzyme sets on the saccharification of wheat straw and sugarcane bagasse. | plant-degrading enzymes can be produced by fungi on abundantly available low-cost plant biomass. however, enzymes sets after growth on complex substrates need to be better understood, especially with emphasis on differences between fungal species and the influence of inhibitory compounds in plant substrates, such as monosaccharides. in this study, aspergillus niger and trichoderma reesei were evaluated for the production of enzyme sets after growth on two "second generation" substrates: wheat st ... | 2014 | 25116172 |
| adsorption of enzyme onto lignins of liquid hot water pretreated hardwoods. | the adsorption of cellulase enzymes onto lignin is shown to be non-productive and therefore reduces enzymatic hydrolysis of liquid hot water pretreated cellulose. among the enzyme components of trichoderma reesei cellulase cocktail, β-glucosidase showed the strongest adsorption onto lignin. only 2-18% of the initial β-glucosidase activity remained in the supernatant while 50-60% of cellobiohydrolase and endoglucanase activities were recovered after incubation with lignin. by increasing the ph to ... | 2015 | 25116138 |
| an antifungal role of hydrogen sulfide on the postharvest pathogens aspergillus niger and penicillium italicum. | in this research, the antifungal role of hydrogen sulfide (h2s) on the postharvest pathogens aspergillus niger and penicillium italicum growing on fruits and under culture conditions on defined media was investigated. our results show that h2s, released by sodium hydrosulfide (nahs) effectively reduced the postharvest decay of fruits induced by a. niger and p. italicum. furthermore, h2s inhibited spore germination, germ tube elongation, mycelial growth, and produced abnormal mycelial contraction ... | 2014 | 25101960 |
| chemical compositions and biological activities of the essential oils of beilschmiedia madang blume (lauraceae). | the present study aimed to examine the chemical compositions of the essential oils of beilschmiedia madang and their antioxidant, antibacterial, antifungal, anticholinesterase and anti-tyrosinase activities. the major constituents of the essential oils of leaf and bark of b. madang were δ-cadinene (17.0 and 20.5 %), β-caryophyllene (10.3 and 6.7 %), α-cubebene (11.3 and 15.6 %), and α-cadinol (5.8 and 10.6 %). the essential oils were screened for their antioxidant activities using 1,1-diphenyl-2 ... | 2015 | 25098422 |
| production of 1,5-anhydro-d-fructose by an α-glucosidase belonging to glycoside hydrolase family 31. | α-1,4-glucan lyases [glycoside hydrolase family (gh) 31] catalyze an elimination reaction to form 1,5-anhydro-d-fructose (af), while gh31 α-glucosidases normally catalyze a hydrolytic reaction. we determined that a small amount of af was produced by gh31 aspergillus niger α-glucosidase from maltooligosaccharides by elimination reaction, likely via an oxocarbenium ion intermediate. | 2014 | 25093855 |
| comparison of species composition and fumonisin production in aspergillus section nigri populations in maize kernels from usa and italy. | fumonisin contamination of maize is considered a serious problem in most maize-growing regions of the world, due to the widespread occurrence of these mycotoxins and their association with toxicosis in livestock and humans. fumonisins are produced primarily by species of fusarium that are common in maize grain, but also by some species of aspergillus sect. nigri, which can also occur on maize kernels as opportunistic pathogens. understanding the origin of fumonisin contamination in maize is a ke ... | 2014 | 25087207 |
| synthesis, spectroscopic, anticancer, antibacterial and antifungal studies of ni(ii) and cu(ii) complexes with hydrazine carboxamide, 2-[3-methyl-2-thienyl methylene]. | schiff's base ligand(l) hydrazine carboxamide, 2-[3-methyl-2-thienyl methylene] and its metal complexes have been synthesized and characterized by elemental analysis, molar conductance, various spectroscopic techniques such as electronic, ir, (1)h nmr, mass, epr. molar conductance of complexes in dmf solution corresponds to non-electrolyte. complexes have general composition [m(l)2x2], where m=ni(ii) and cu(ii), x=cl(-), no3(-), ch3coo(-) and ½so4(2-). on the basis of above spectral studies, an ... | 2015 | 25087168 |
| the transcriptional activators arar and xlnr from aspergillus niger regulate expression of pentose catabolic and pentose phosphate pathway genes. | the pentose catabolic pathway (pcp) and the pentose phosphate pathway (ppp) are required for the conversion of pentose sugars in fungi and are linked via d-xylulose-5-phosphate. previously, it was shown that the pcp is regulated by the transcriptional activators xlnr and arar in aspergillus niger. here we assessed whether xlnr and arar also regulate the ppp. expression of two genes, rpia and talb, was reduced in the δarar/δxlnr strain and increased in the xylulokinase negative strain (xkia1) on ... | 2014 | 25086261 |
| optimization of ellagitannase production by aspergillus niger gh1 by solid-state fermentation. | ellagic acid is one of the most bioactive antioxidants with important applications in pharmaceutical, cosmetic, and food industries. however, there are few biotechnological processes developed for its production, because it requires precursors (ellagitannins) and the corresponding biocatalyst (ellagitannase). the aim of this study was to optimize the culture conditions for ellagitannase production by aspergillus niger in solid-state fermentation (ssf). the bioprocess was carried out into a colum ... | 2015 | 25085574 |
| a new in vitro method to detect growth and ochratoxin a-producing ability of multiple fungal species commonly found in food commodities. | the aim of the study was to develop a new screening method to detect growth and ochratoxin a (ota) production by multiple fungi growing in a small quantity of culture media, using microtiter plates. eight ochratoxigenic species were included in the study. the strains were inoculated in sterile 96-well flat-bottom microtiter plates containing yeast extract sucrose broth and czapek yeast extract broth and incubated at 25 °c. growth was daily monitored by absorbance measurements for 4 days and exte ... | 2014 | 25084669 |
| effects of post-harvest treatment using chitosan from mucor circinelloides on fungal pathogenicity and quality of table grapes during storage. | the aim of this study was to extract chitosan (chi) from mucor circinelloides ucp 050 grown in a corn steep liquor (csl)-based medium under optimized conditions and to assess the efficacy of the obtained chi to inhibit the post-harvest pathogenic fungi aspergillus niger urm 5162 and rhizopus stolonifer urm 3482 in laboratory media and as a coating on table grapes (vitis labrusca l.). the effect of chi coating on some physical, physicochemical and sensory characteristics of the fruits during stor ... | 2014 | 25084665 |
| protein redesign by learning from data. | protein redesign methods aim to improve a desired property by carefully selecting mutations in relevant regions guided by protein structure. however, often protein structural requirements underlying biological characteristics are not well understood. here, we introduce a methodology that learns relevant mutations from a set of proteins that have the desired property and demonstrate it by successfully improving production levels of two enzymes by aspergillus niger, a relevant host organism for in ... | 2014 | 25082898 |
| application of silica nanoparticles in maize to enhance fungal resistance. | in this study, maize treated with nanosilica (20-40 nm) is screened for resistance against phytopathogens such as fusarium oxysporum and aspergillus niger and compared with that of bulk silica. the resistivity is measured for disease index and expression of plant responsive compounds such as total phenols, phenylalanine ammonia lyase, peroxidase and polyphenol oxidase. the results indicate that nanosilica-treated plant shows a higher expression of phenolic compounds (2056 and 743 mg/ml) and a lo ... | 2014 | 25082220 |
| talaflavuterpenoid a, a new nardosinane-type sesquiterpene from talaromyces flavus. | talaflavuterpenoid a (1), a new nardosinane-type sesquiterpene, was isolated from the wetland soil-derived fungus talaromyces flavus byd07-13, and its structure was elucidated on the basis of hr-ms, nmr, and x-ray diffraction analysis. the absolute configuration of 1 was established by comparing the experimental electronic circular dichroism (ecd) spectrum with the calculated ecd spectra. its cytotoxic effects on five human tumor cell lines (hl-60, smmc-7721, a-549, mcf-7, and sw480), and antimi ... | 2014 | 25082104 |