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[change in ratio between citric and oxalic acid in aspergillus niger upon exposure to mutagenic factors].stepwise selection of aspergillus niger producing citric acid in highly buffered molassa media was carried on in order to decrease the biosynthesis of oxalic and gluconic acids using the following mutagenic factors: diethyl sulfate, thiophosphamide, cyclophosphan, n-methyl-n'-nitro-n-nitrosoguanidine (ng), 5-fluorouracil, 1,4-bis-diazoacetylbutane, and uv. the ratio between the acids changed in mutants obtained upon the combined treatment with uv and ng. mutants producing twice as less oxalic ac ...1979119143
[on the serodiagnosis of aspergillosis: comparison of various aspergillus extracts (author's transl)]. 1979121207
characterization of a rennin-like enzyme produced in submerged cultures of aspergillus niger. 1979121622
intra- and extracellular forms of alpha-glucosidase from aspergillus niger. 1979380468
studies on the mode of action of 5-fluorocytosine in aspergillus species.the mode of action of 5-fluorocytosine (5-fc) was studied in three isolates of pathogenic aspergillus with varying degrees of susceptibility to the drug. distribution studies showed that susceptibility or resistance to 5-fc was not dependent on uptake of the drug. while only a small percentage of the total 5-fc taken up was found in the rna fraction of the cells, most remained in the acid-soluble intracellular pool. 5-fc, 5-fluorouracil (5-fu) and 5-fluorodeoxyuridine monophosphate (5-fd-ump) we ...1979156110
esr characterization of chitins and chitosans. 1979226089
preliminary report on toxigenic fungal isolates of aspergillus niger in market foods and foodstuffs.14 out of 22 fungal isolates of a. niger from market foods and foodstuffs were toxigenic to weanling rats. approximately 2/3 of these cultures were highly toxigenic. histopathologic changes were observed mostly in kidney with necrosis of the tubular epithelium and to a less extent in liver with centrolobular necrosis of the hepatic cells.1979437045
synthesis of methyl 1-(3,4-dichlorobenzyl)-hexahydro-2,3-dioxo-4-pyridinecarboxylate.methyl 1-(3,4-dichlorobenzyl)-hexahydro-2,3-dioxo-4-pyridinecarboxylate was prepared by alkylation of methyl hexahydro-2,3-dioxo-4-pyridinecarboxylate with alpha,3,4-trichlorotoluene. the compound showed significant antifungal activity when challenged by a spectrum of pathogenic yeast species in a minimum inhibitory concentration test. it was active against some dermatophytic fungi and aspergillus niger.1979438987
[on the contamination of the citric acid fermentation. i. the contamination by representatives of enterobacteriaceae (author's transl)].among the most dangerous bacterial contaminations of the citric acid fermentation is the so-called brown contamination, which is called forth by non pathogenic representatives of enterobacteriaceae like escherichia coli, aerobacter aerogenes, enterobacter cloacae and proteus vulgaris, while other bacteria do not produce the mentioned contamination. there is given a comprehensive characterization of the brown contamination. enterobacteriaceae interrupt the growth of the mycelium of the mold asper ...1979398647
[on the contamination of the citric acid fermentation. ii. sodium 5-nitrofurylacrylate as an antiseptic against representatives of the enterobacteriaceae (author's transl)].in context with the first communication which deals with the characterization of the so-called brown contamination called forth by enterobacteriaceae in the citric acid fermentation of molasses, the present paper describes the screening of antiseptic substances able to suppress the contamination mentioned without damaging the normal growth of the producing mould aspergillus niger and its ability of producing citric acid. among the substances tested the sodium salt of 5-nitrofurylacrylic acid (5- ...1979398648
characterization of rennin-like enzyme produced in submerged cultures of aspergillus niger. 1979400533
antibiotic properties of lignin components.inhibitory effects of compounds with guaiacyl and syringyl structure, representing the structure of native lignin, were studied on model cultures of bacteria, yeasts, yeast-like microorganisms and moulds. isoeugenol exhibited the most pronounced inhibitory effect on growth of the studied microorganisms.1979389763
mutagens in coffee and tea.coffee prepared in the usual way for drinking contains a substance(s) that is mutagenic to salmonella typhimurium ta100 without mammalian microsomal enzymes. one cup of coffee (200 ml) contains mutagen(s) inducing 1.4-4.6 x 10(5) revertants under standard conditions. instant coffee too is mutagenic to ta100 and one cup of instant coffee prepared from 1 g of coffee powder and 200 ml of water induced 5.6-5.8 x 10(4) revertants of ta100. caffeine-free instant coffee also has similar mutagenicity. a ...1979390384
[changes in rabbit lungs after exposure to grain and hay dust and in intravenous injections of aspergillus niger and candida albicans (author's transl)]. 1979391392
the saccharifying ability of the cellulase complex of talaromyces emersonii and comparison with that of other fungal species. 1979456725
orlandin: a nontoxic fungal metabolite with plant growth inhibiting properties. 1979447931
purification and some properties of five endo-1,4-beta-d-xylanases and a beta-d-xylosidase produced by a strain of aspergillus niger.five different xylanases and a beta-d-xylosidase in the culture medium of aspergillus niger have been purified to homogeneity from 13- to 52-fold by a procedure of gel and hydroxylapatite chromatography. the strain was isolated from soil of the african equatorial forest. gel chromatography of the purified enzymes indicated that three of the xylanases have molecular weights of 31,000 and the other two xylanases have molecular weights of 50,000. beta-d-xylosidase has a molecular weight of 78,000. ...1979455113
infection of a burn wound by aspergillus niger. gross appearance simulating ecthyma gangrenosa.growth of aspergillus niger on a burn wound clinically simulated the early (hemorrhagic) phase of an invasive infection by pseudomonas aeruginosa or ecthyma gangrenosa. wound biopsy for histologic examination and culture readily yielded a definitive diagnosis of noninvasive mycotic infection of the burn wound. pigmentation surrounding mycotic hyphae (otherwise typical for aspergillus spp.) strongly suggested aspergillus niger. cultural data confirmed the diagnosis.1979474499
toxic aspergilli from pistachio nuts.pistachio nut samples taken during various stages of development from orchards in iran, showed that contamination with fungi occurred mainly during the later stages of nut development. members of the genera aspergillus and penicillium occurred most frequently. of the aspergilli, the species a. niger, a. flavus and a. fischeri var. spinosus occurred most frequently, followed by a. terreus, a. tamarii and a. nidulans. twenty-two isolates comprising 13 species were tested for toxicity to ducklings. ...1979481560
mycological survey of selected health foods.a survey was conducted to compare the total viable fungal content and the number of different mold species encountered in 10 types of health foods labeled organically grown and in the same foods without such a label. the foods were wheat flour, corn meal, brown rice, figs, split peas, pinto beans, soybeans, walnuts, pecans, and peanuts. results showed no consistent difference in either the total viable fungal content or the number of different mold species encountered between the labeled and unl ...1979453829
synthesis of various kinds of esters by four microbial lipases.ester synthesis by microbial lipases, using homogeneous enzyme preparations, were investigated. the amount of synthesized ester was estimated by alkalimetry, and products were identified by thin-layer chromatography and infrared spectroscopy. lipases from aspergillus niger, rhizopus delemar, geotrichum candidum and penicillium cyclopium synthesized esters from oleic acid and various primary alcohols. only geotrichum candidum lipase synthesized esters of secondary alcohols. esters of tertiary alc ...1979508776
diagnostic value of calcium oxalate crystals in respiratory and pleural fluid cytology. a case report.a 56-year-old man presented with massive, left-sided pleural empyema. sputa, pleural fluids, a bronchial washing and a bronchial biopsy revealed acute inflammatory exudate and numerous birefringent calcium oxalate crystals. one pleural fluid also showed occasional mycelia and rare conidiophores of aspergillus niger. the fungus was abundantly cultured from all cytology specimens. other oxalosis-related conditions were not identified in this patient. the finding of calcium oxalate crystals associa ...1979285553
mutagenic effects of saccharin on aspergillus niger. 1979521793
synthesis and use of specifically tritiated dethiobiotin in the study of biotin biosynthesis by aspergillus niger. 1979440116
[microorganisms of the upper layers of the atmosphere].samples for microbiological studies were taken at a high altitude using meterological rockets with specially constructed analyzers so that no extraneous microorganisms could get into them before sampling. the analyzers were sterilized by gamma-irradiation before sampling. for the first time, microscopic fungi, bacteria and mycobacteria were detected at a height of 48--77 km. five among six of the microbial species were found to contain pigments. the prevalence of these forms at high altitudes su ...1979530135
studies of the carbohydrate residues of glycoproteins by natural abundance carbon 13 nuclear magnetic resonance spectroscopy. glucoamylase from aspergillus niger. 1979374412
immobilization of anthranilate hydroxylase from aspergillus niger. 1979535919
manganese deficiency leads to elevated amino acid pools in citric acid accumulating aspergillus niger.free amino acid pools have been investigated in a citric acid accumulating strain of aspergillus niger during batch growth under manganese sufficient and deficient conditions by means of an improved chromatographic method. studies on the mycelial content of several nitrogenous compounds under manganese sufficient and deficient conditions showed that manganese deficiency resulted in lower amino acid pool sizes during trophophase and considerable accumulation during idiophase, and in a reduction o ...1979543776
studies on the inhibitory effects of zinc heptanoate on microorganisms.inhibitory effect of zinc heptanoate was observed on different cultures of bacteria and fungi. growth of all the bacteria was inhibited by the compound. greatest inhibition was seen in the case of staphylococcus albus, streptococcus pyogenes, shigella dysenteriae, shigella sonnei, shigella flexneri, salmonella typhi, s. paratyphi a, s. paratyphi b, vibrio cholerae, corynebacterium diphtheriae, and e. coli whereas least inhibition was found in the case of staphylococcus aureus. in triethanolamine ...1979553835
[substrate thermostabilization of soluble and immobilized glucoamylase].a new kinetic approach to the study of enzyme thermal inactivation in the presence of a substrate, which influences the rate of inactivation has been developed. the method was applied to investigation of inactivation kinetics of soluble and porous silica-immobilized glucoamylase. it was found that the binding of a substrate (maltose or maltodextrines star-dri 24-r) increases the thermal stability of glucoamylase, the stabilizing effect being more pronounced in the case of the soluble enzyme (40- ...1979380665
invasive aspergillosis presenting as pericarditis and cardiac tamponade.a 38-year-old leukemic patient developed pericarditis and cardiac tamponade due to aspergillus niger one month after undergoing bone marrow transplantation. she failed to improve even though amphotericin b and rifampin therapy had been initiated before infection was evident. her unique case illustrates both the unusual presentations of invasive aspergillosis and the difficulty of diagnosing and treating this increasingly common disease.1979389577
the thermostability of glucoamylase immobilized in different ways has a certain limit.thermostability of glucoamylase from aspergillus niger is increased both by immobilization and substrate binding. however, the total superimposed stabilization effect at a given condition is apparently restricted by a certain limit which hardly depends on the mode of immobilization of the enzyme, and is determined mostly by the enzyme-substrate complex formation.1979387088
the effect of beta-glucuronidase and chitinase on the cell wall of aspergillus niger and aspergillus fumigatus.the effects of beta-glucuronidase and chitinase have been tested on the hydrolysis of the cell walls of the economically important fungi, aspergillus niger and aspergillus fumigatus. the extent of wall hydrolysis was measured by assaying for total reducing sugars, n-acetyl sugars and protoplast production. maximum reducing sugar release was attained after 40 min incubation, both with beta-glucuronidase supplemented with chitinase and beta-glucuronidase alone, whereas n-acetyl sugar release reach ...1979396451
[toxic properties and the effect on the body of the a. niger fungi isolated from vegetable food products]. 1979456900
use of immobilized enzymes in automated clinical analysis: determination of uric acid and glucose using immobilized enzymes in column form.we studied the use of immobilized enzymes, covalently bound to alkylaminosilane derivative of porous glass, to automated clinical analysis on uric acid and glucose in blood, serum and urine. a microcolumn with an immobilized enzyme was prepared and used in an autoanalyzer i continuous flow system. uricase (ec 1.7.3.3) from candida utilis and glucose oxidase (ec 1.1.3.4) from aspergillus niger were immobilized for the determination of uric acid and glucose, respectively. hydrogen peroxide produce ...1979527227
conjugation of glucose oxidase from aspergillus niger and rabbit antibodies using n-hydroxysuccinimide ester of n-(4-carboxycyclohexylmethyl)-maleimide.glucose oxidase from aspergillus niger was conjugated with rabbit immunoglobulin g or its monovalent fragments (fab'). the enzyme was treated with n-hydroxysuccinimide ester of n-(4-carboxycyclohexylmethyl)-maleimide to introduce maleimide groups, which were then allowed to react with thiol groups of reduced igg or fab'. more than 40% of immunoglobulin g, fab' and enzyme used could be conjugated without self-coupling. the enzyme activity decreased about 26 and 15% upon conjugation with immunoglo ...1979574817
[purification of main saponin from tuber of ficaire (ficaria ranunculoides moench) by a strain of aspergillus niger (author's transl)]. 1979547816
anthranilate hydroxylase, an iron enzyme, from aspergillus niger. 1979548462
electron microscopy of some rock phosphate dissolving bacteria and fungi.bacteria pseudomonas striata, bacillus polymyxa, b. megaterium and b. pulvifaciens, and fungi aspergillus awamori, a. niger and penicillium digitatum dissolve tricalcium phosphate and, much less, mussorie and udaipur rock phosphate. the solubilizing power of fungi was higher than that of bacteria, the highest being with a. awamori and a. niger, and with p. striata. electron microscopy of the various cultures showed an electron-dense layer on the bacterial surface after negative staining. the siz ...1979527907
liberation of protoplasts from the yeast-like form of paracoccidioides brasiliensis by enzymatic treatment.spherical and osmotically sensitive protoplasts were released from cultures of the yeast-like form of paracoccidioides basilienisis strain ivic pb9 through the action of a mixture of crude enzyme preparations: alpha and beta-glucanases and chitinase, obtained from culture filtrates of cladosporium resinae, basidiomycete qm 806 and streptomyces sp respectively. the highest efficiency of protoplast liberation was achieved when each crude enzyme preparation was used at 1 mg/ml.1979531719
toxic naphtho-gamma-pyrones from aspergillus niger. 1979544666
sodium hydroxide sterilzation of intraocular lenses.intraocular lenses of polymethylmethacrylate contaminated with various inocula of bacteria, spores, and fungi were sterilized by modifications of the ridley method using sodium hydroxide. positive cultures were obtained from iris-supported lenses contaminated with concentrations of approximately 1 x 10(6) organisms per milliliter after one hour in 10% naoh. no positive cultures were obtained when lenses remained in 10% naoh for three hours, a time corresponding to a 1 x 10(-6) probability of sur ...1979484686
[use of ethonium in treating fungal diseases of the external ear]. 1979494767
reaction of fungal amine oxidase with beta-bromoethylamine.beta-br-ethylamine is both a substrate and an irreversible inhibitor of amine oxidase from aspergillus niger. the enzyme catalyzes the nonoxidative elimination of hbr from beta-br-ethylamine to form acetaldehyde. beta-br-ethylamine meets several criteria for an irreversible substrate analog or suicide inhibitor. 1) it inactivates the oxidized enzyme, but not the reduced enzyme. 2) the michaelis constant for beta-br-ethylamine in the elimination reaction showed a similar magnitude to that of the ...1979500616
[isolation of cultures of microorganisms growing in the presence of hydrogen peroxide]. 1979502915
glycosylation of nascent polypeptide chains in aspergillus niger. 1979507840
an attempt to establish a model for the rhythmic phenomena affecting the growth or sporulation of certain fungi.a simple two-variable mathematical model is proposed, able to acount for periodic variations relating to growth in podospora anserina and fructification in aspergillus niger. this basic model is open to generalization.1979520098
[localised aspergillosis and oxalosis of the lung caused by aspergillus niger: soil of ornamental plants as a reservoir of aspergilli (case report) (author's transl)].haemoptysis occurred in a 64-year-old man with a history of pulmonary tuberculosis, negative for m. tuberculosis. precipitating antibodies to aspergillus niger were found in serum and the fungus found in sputum. autopsy revealed aspergilloma, colonization of the pulmonary cavity by a. niger and local oxalosis if the cavity wall. ornamental plant soil containing a. niger conidia, as well as the air in the patient's living-room, was the probable source of infection.1979467276
[formation of volutin inclusions in the mycelium of aspergilli growing on media with hydrocarbons]. 1979440149
the preparation and partial characterization of antigenic fractions obtained from the mycelial walls of several aspergillus species.extracts with immunological activity were prepared from aspergillus fumigatus, a. flavus, a. terreus, a. niger and a. nidulans. in each case crude mycelial wall was extracted with an aqueous solution of triton x-100 giving detergent-soluble material. further fractionation was achieved by removing the detergent from this solution; the resultant precipitate was removed by centrifugation, and the aqueous supernatant was used as a source of soluble antigens. the sensitivity of these preparations was ...19806157778
a spectrophotometric method for the determination of glucose-6-phosphatase activity. 19806244755
microbial alpha-amylase production from low quality potatoes. 19806161891
drug therapy of aspergillus otitis externa. 19806451857
interaction between aspergilli and streptomycetes in the soil of potted indoor plants: a preliminary report (contribution to the epidemiology of human aspergillosis).the soil of potted ornamental plants as a reservoir for aspergillus species pathogenic for man is of epidemiological and ecological interest. isolation of a. niger as the sole hyphomycete from the soil of potted african violets (saintpaulia ionantha, gesneriaceae), prompted us to look for a. inger on the surface of the roots of this plant. small pieces of the roots were inoculated in the nutrient-free agar-gel with and without antibiotics. on the antibiotic-free gel, a dense growth of streptomyc ...19806769046
[effect of metabolites of aspergillus genus fungi on water absorption in the large intestine of guinea pigs]. 19806769241
air-borne fungi in the air of barcelona (spain). iii. the genus aspergillus link.during a survey on the presence of species of the genus aspergillus in the air of the city of barcelona (spain), the following species were identified: aspergillus flavus link, a. niger van tieghem, a. fumigatus fresenius, a. clavatus desmazières, a. terreus thom, a. chevalieri (mang.) thom et church, a. niveus bloch, emend. thom et church, a. ochraceus wilhelm, a. versicolor (vuillemin) tiraboschi, and a. amstelodami (mang.) church et thom.19806770271
accumulation of 2-oxo acids in mutants of aspergillus niger requiring lysine.mutants of aspergillus niger 194a and 178 requiring lysine differ from the original prototrophic strain k10 and from each other on the course of accumulation of organic acids. in both mutants less citric acid accumulates during the first phase of cultivation but considerably more 2-oxoglutarate and 2-oxoadipate accumulate than in the original strain. whereas in the 194a mutant this state remains unchanged also during the second phase of cultivation, in the 178 mutant oxo acids are degraded and c ...19806774936
ureide interactions in thiourea toxicity in aspergillus niger. 19806778816
high-yield method for immobilization of enzymes.two types of polyethylenimine-coated glass microbeads (13-44 micrometer) were synthesized and used for the immobilization of glucose oxidase from aspergillus niger and catalase from a. niger and beef liver. the two types of beads were distinguishable by differences in their surface topography. immobilizations were performed by adsorption followed by treatment with glutaraldehyde. the immobilized-enzyme activities per unit support of all of the enzymes tested were compared with and found to be su ...19806766330
glycoenzymes: an unusual type of glycoprotein structure for a glucoamylase.glucoamylase, (1 leads to 4)(1 leads to 6)-alpha-d-glucan glucohydrolase (ec 3.2.1.3), hydrolyzes starch and glycogen completely to d-glucose and is used industrially in the manufacture of d-glucose from starch. the enzyme is elaborated by many types of fungi and occurs in two isoenzymic forms (glucoamylase i and glucoamylase ii) in extracts from certain fungi. the isoenzymes from aspergillus niger are glycoenzymes containing d-mannose, d-glucose, and d-galactose as integral structural component ...19806774807
antimicrobial activities of some antineoplastic and other withanolides. 19806994645
biosynthesis of d-alanyl-lipoteichoic acid: characterization of ester-linked d-alanine in the in vitro-synthesized product.d-alanyl-lipoteichoic acid (d-alanyl-lta) contains d-alanine ester residues which control the ability of this polyer to chelate mg(2+). in lactobacillus casei a two-step in vitro reaction sequence catalyzed by the d-alanine-activating enzyme and d-alanine:membrane acceptor ligase incorporates d-alanine into membrane acceptor. in this paper we provide additional evidence that the in vitro system catalyzes the covalent incorporation of d-[(14)c]alanine into membrane acceptor which is the poly([(3) ...19806772629
[effect of progressive chemical modification on the activity and thermal stability of soluble and immobilized glucoamylase].the chemical modification of glucoamylase from aspergillus niger with acryloylchloride has been studied. it was shown that the first 60--70% of the total amount of available amino groups of the enzyme readily interact with the reagent: the activity and thermal stability of the resulting soluble acryloylglucoamylase are only slightly less than those of the native enzyme. the modification of the remaining 30% of the total amount of amino groups is less intensive and results in a sufficient loss of ...19806783128
characterization of antigens from aspergillus fumigatus. v. reactivity in immunodiffusion tests with serums from patients with aspergillosis caused by aspergillus flavus, a. niger, and a fumigatus.antigen-rich mycelial extract (me) and fractions of aspergillus fumigatus were compared with culture filtrates of a. fumigatus. a. flavus, and a. niger for their abilities to detect antibody in serums from patients with confirmed aspergillosis caused by these fungi. in double immunodiffusion tests, homologous antigen preparations were more effective than heterologous preparations in detecting precipitins. mycelial extract of a. fumigatus was more effective than a. fumigatus culture filtrate in ...19806776857
uptake of lysosomal enzymes by human fibroblasts: lack of uptake of fungal or plant glycosidases in comparison with a mammalian enzyme.alpha-galactosidase from human placenta was actively taken up by cultured fibroblasts from patients with fabry's disease whereas the enzyme from the fig was not absorbed. alpha-glucosidase from two microbiological sources. a. fumigatus and a. niger, was not taken up by cultured fibroblasts from patients with pompe's disease.19806787335
structural information from molecular connectivity 4 chi pc index.the molecular connectivity 4 chi pc index was examined for its ability to describe uniquely molecules containing substituted benzene rings. the subgraphs comprising this index were shown to encode information about the number, placement, and type of ring substituents. several examples illustrate the ability of the index to describe structure-influencing properties.19806774081
[ocular mycoses as a complication of hormonal therapy]. 19806989089
leaching of a silicate and carbonate copper ore with heterotrophic fungi and bacteria, producing organic acids.leaching of metals with carbon-heterotrophic microorganisms and organic acids as active agents has been performed with timna copper ore containing 1.1% copper as carbonates and silicate and with some other metal oxides. aspergillus niger surface cultures on a 14% sucrose fermentation medium yeilded 87.3% of total copper from 10% suspension within 2 weeks of leaching. on sulfite liquor as an industrial waste product a. niger solubilized 73.8% cu within 4 weeks. in percolators with 14% sucrose med ...19807222743
[role of microorganisms in the destruction of spodumene].a broad spectrum of microorganisms has been shown to be involved in the destruction of spodumene, a typical mineral of lithium pegmatites. the following microorganisms are most active: the microscopic fungi penicillium notatum and aspergillus niger, the thiobacilli thiobacillus thiooxidans, and the so-called "silicate" slime forming bacterium bacillus micilaginosus n. sp. siliceus. spodumene destruction is accompanied with lithium, aluminium and silicon being transferred into solution. the activ ...19806995818
immunogenic properties of pr8 influenza virus after treatment with acid protease.brief exposure of pr8 influenza virus at ph 4.5 to extracts of aspergillus niger abolished the red cell agglutinating and infectious capacities of the virus without significantly decreasing its antigenicity or immunogenicity. the results of gel electrophoresis indicated that the ha1 portion of the viral hemagglutinin had been degraded by treatment of virus with aspergillus extract. the immunogenicity in mice of virus that had been exposed to a. niger extract appeared to be very broad, since vacc ...19807009489
the effect of calcium on protoplast release from species of aspergillus.the effect of various osmotica and lytic enzyme treatments, on the release of stable protoplasts from aspergillus niger and two strains of aspergillus fumigatus, has been investigated. most osmotica supported protoplast release at a concentration of 0.6 m except for cacl2 and ca(no3)2. supplementation of 0.6 m kcl with 0.2 m cacl2 enhanced release from both species, but depended upon strain of organism, concentration of osmoticum and enzyme system employed. more protoplasts were released at low ...19807010082
proteolytic action of aspergillus niger extract on influenza virus. 19807016806
'an intracavitary fungus ball composed of syncephalastrum'. 19807189103
[interrelationship between fungi used as test cultures with regard to methods of testing the biostability of products of medical technology]. 19807189419
the influence of some microorganisms on the in vitro multiplication of histomonas meleagridis. 19807032080
formation of nicotinamide ribose diphosphate ribose, a new metabolite of the nad pathway, by aspergillus niger. 19807374464
isolation, purification and partial characterization of alkaline phosphatase from aspergillus niger. 19807380079
isolation and characterization of a chlorogenic acid esterase from aspergillus niger.the isolation and characterization of a specific chlorogenic acid esterase is described. the enzyme activity is measured by determination of the hydrolysis product caffeic acid. the enzyme had been concentrated by means of ultrafiltration and column-chromatography. the ph- and temperature optimum were 6.5 and 45 degrees c respectively. divalent cations were not required for the enzyme activity. as other esterases, this enzyme is inhibited by di-isopropyl-phosphorofluoridate. the km-value is 0.70 ...19807385941
pyridine nucleotide levels and ratios in aspergillus niger.a reliable method for the extraction and assay of pyridine nucleotides (nad, nadh, nadp, and nadph) in filamentous fungi is presented. the method is applied to a study of the physiology of citric acid accumulation by aspergillus niger. the measurement of "catabolic reduction change" (nadh/(nad + nadh)) and "anabolic reduction charge" (nadph/(nadp + nadph)) as a means of characterizing the metabolic state is discussed.19807407716
influence of manganese on morphology and cell wall composition of aspergillus niger during citric acid fermentation.morphology and cell wall composition of aspergillus niger were studied under conditions of manganese sufficient or deficient cultivation in an otherwise citric acid producing medium. omission of mn2+ (less than 10(-7) m) from the nutrient medium of aspergillus niger results in abnormal morphological development which is characterized by increased spore swelling, and squat, bulbeous hyphae. fractionation and analysis of manganese deficient cell walls revealed increased chitin and reduced beta-glu ...19807458536
conjugation of enzymes to immunoglobulins using dimaleimides.a method is described for coupling enzymes to immunoglobulins using sulphydryl derivatives of the proteins and a dimaleimide which is relatively water-soluble. parameters affecting the performance of the conjugates have been examined including level of sulphydryl incorporation, ratio of enzyme/immunoglobulin and nature of dimaleimide used. peroxidase-immunoglobulin conjugates made by the dimaleimide method have been compared with those made by the periodate oxidation method and found to have a s ...19806988011
the effects of tunicamycin on protein glycosylation in mammalian and fungal systems. 19807371958
metabolite in diagnosis of pulmonary aspergillosis and oxalic acid factor. 19807352707
chelating, film-forming, and coagulating ability of the chitosan-glucan complex from aspergillus niger industrial wastes.waste mycelia of aspergillus niger from a citric acid production plant are simply treated with boiling 30-40% naoh aqueous solutions for 4-6 hr to obtain the insoluble chitosan-glucan complex whose infrared, esr, and x-ray diffraction spectra are reported. a number of transition- and post-transition-metal ions are chelated and collected by chitosan-glucan with higher yields than by animal chitosan. immediate flocculation occurs upon mixing chitosan-glucan dispersions with alginate and polymolybd ...19807362863
significance of aspergillus species isolated from respiratory secretions in the diagnosis of invasive pulmonary aspergillosis.to determine the significance of aspergillus species isolated from sputum or other respiratory secretions with respect to the diagnosis of invasive pulmonary aspergillosis, the clinical records and radiographs of all patients whose respiratory secretion cultures yielded an aspergillus species between 1972 and 1978 were reviewed. all known predispositions to invasive aspergillosis, e.g., presence of cancer or granulocytopenia, and therapy with corticosteroids, antibiotics, and cytotoxic drugs, we ...19807372799
antifungal properties of n-alkanols, alpha, omega-n-alkanediols, and omega-chloro-alpha-alkanols.fourteen n-alkanols (c1-c12, c14, and c16), 13 alpha,omega-n-alkanediols (c2-c12, c14, and c16), and 13 omega-chloro-alpha-alkanols (c2-c12, c14, and c16) were tested against aspergillus niger, trichoderma viride, and myrothecium verucaria in sabouraud dextrose agar at ph 4.0 and 5.6. toxicity to candida blbicans, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. the fungitoxicity of these alcohols was ...19807373528
[effect of detergents on the hydroxylation of indolyl-3-acetic acid by an aspergillus niger culture].a possibility to increase the hydroxylating activity of aspergillus niger ibfm f-212 under the action of detergents was studied during transformation of indolyl-3-acetic acid (iaa). the following non-ionogenic surface-active compounds were mainly used: tweens, spans, polyethyleneglycol (peg-400). the effect of the detergents was studied at the stages of growth, transformation and preincubation. at the stage of growth, the best effect was produced by tween-80. at the stages of transformation and ...19807412617
regulation of citrate synthase from the citric acid-accumulating fungus, aspergillus niger.citrate synthase (citrate oxaloacetate-lyase (pro-3s-ch2coo leads to acetate-coa), ec 4.1.3.7) was purified 66-fold from cell-free extracts of a citric acid producing strain of aspergillus niger. the enzyme is labile at low ionic strength, but can effectively be stabilized by k+, oxaloacetate or glycerol. it has a molecular weight of 80 000 and an optimum ph of 8.5. the enzyme is activated by monovalent cations in dilute buffer solutions, and inhibited by mg2+ independent of the buffer molarity. ...19807417457
the influence of ultrasonic waves, alone and in presence of certain salts on the microbial production of citric acid by aspergillus niger.the influence of ultrasonic waves, alone and in presence of different (0.1, 0.5, and 1.0%) concentrations of nacl, kh2po4 and nh4cl on the microbial production of citric acid by aspergillus niger was studied. it was observed that in presence of sonic waves alone the yield of citric acid was generally better than that of control. besides, some morphological changes in the diameter of the conidiophore of the of the mould was also noted. the effect of sonics in presence of nacl was inconspicuous si ...19807456808
[obtaining pectinolytic mutants of aspergillus niger]. 19807467410
rhizosphere fungi of five plants in egypt.75 species which belong to 25 genera were collected during this investigation. all of these fungi were previously isolated from egyptian soils and other sources. the total count and the number of genera and species in the rhizosphere soil of the 5 plants were regularly higher than in non-rhizosphere soil. the composition of the rhizosphere fungus flora was considerably influenced by the type and the age of the plant. the most prevalent fungi in the rhizosphere of the test plants after 45, 90, an ...19807424047
biochemical studies on acetate non-utilizing (acu) mutants of aspergillus niger. 19807429599
purification of an alpha-l-arabinofuranosidase from aspergillus niger by substrate affinity chromatography. 19807430310
[effect of products of thermophilic methane fermentation on the conidial activation and citric acid biosynthesis by the fungus aspergillus niger].the effect of products of thermophilic methane fermentation (ptmf) and their hydrolyzates on the conidial germination and citric acid bioysnthesis by the fungus aspergillus niger when cultivated on the synthetic and molasses media was investigated. an addition of the above products increased the amount of germinated conidia on the synthetic medium by 1.2--2 times and on the molasses medium by 1.1--1.4 times. the citric acid yeild per unit surface area on the two media grew by 60--100% and 10--20 ...19807433429
purification, properties, kinetics, and mechanism of beta-n-acetylglucosamidase from aspergillus niger.beta-n-acetylglucosaminidase has been purified from an acetone extract of aspergillus niger. the protein has a mr = 149,000. it contains neither mn2+, zn2+, nor cysteine and exhibits no cation requirement for activity. isoelectric focusing separates two isozymes; the major isoenzyme has a pi = 4.4. both isozymes exhibit beta-n-acetylgalactosaminidase and beta-glucosidase, as well as glucosaminidase activity. the mechanism of action of this enzyme has been studied in detail using a variety of sub ...19807440573
[isolation and characterization of an acetylester-hydrolase from aspergillus niger (author's transl)].the characteristic features of an acetic acid esters hydrolyzing enzyme (acetylesterase, ec 3.1.1.16) are described. the ph- and temperature optimum were 7.0 and 40 degrees c respectively. the stability of the enzyme regarding different ph- and temperature conditions was investigated. the molecular weight of the acetylesterase could be determined to 160 000. a small acetic ester hydrolyzing activity was found too with a molecular weight of about 25 000. the activity was not inhibited by addition ...19807445676
[further characterization of a chlorogenic acid hydrolase from aspergillus niger (author's transl)].in addition to our previous paper [1] further characteristics of the chlorogenic acid hydrolase are described. polyacrylamide gel electrophoresis revealed only one band for the purified enzyme. sodium dodecyl-sulfate polyacrylamide gel electrophoresis showed a molecular weight of 60 000, demonstrating four subunits of the enzyme (total molecular weight 240 000). the enzyme is stable in a ph-range of 3.0--8.5 and up to a temperature of 55 degrees c. the temperature coefficient q10 is 1.5, the act ...19807445677
[resistance of microorganisms of upper layers of the atmosphere to ultraviolet radiation and a high vacuum].solar radiation and high vacuum are the main factors affecting the incidence of microorganisms in the stratosphere and mesophere. it has been established in experiments that the conidia of aspergillus niger are most resistant of uv irradiation. the conidia of penicillium, the spores of papulaspora anomala and circinella muscae, and the vegetative cells of micrococcus and mycobacterium are more susceptible to uv radiation. all of the isolated microorganisms have been shown to be resistant to high ...19807392991
infection of a burn wound by aspergillus niger. 19807395812
the relationship between pleurotus ostreatus and aspergillus flavus and the production of aflatoxin.the relationship between pleurotus ostreatus and aspergillus flavus in common mixed culture on various substrates was investiaged. it was found that p. ostreatus, similarly to some other higher fungi, can liquidate colonies of a. flavus. this fungus does not produce aflatoxin and chromatographically similar compounds. on straw, corn cobs, millet and wheat a. flavus produced aflatoxin after a 3-week cultivation. a subsequent cultivation of p. ostreatus led to detoxication of straw and corn cobs b ...19807419131
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