Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| reaction mechanism of 5,8-linoleate diol synthase, 10r-dioxygenase, and 8,11-hydroperoxide isomerase of aspergillus clavatus. | aspergilli express fusion proteins of an animal haem peroxidase domain with fatty acid dioxygenase (dox) activity ( approximately 600 amino acids) and a functional or non-functional hydroperoxide isomerase/cytochrome p450 domain ( approximately 500 amino acids with exxr and gphxclg motifs). 5,8-linoleate diol synthases (lds; ppoa) and 10r-dox (ppoc) of aspergillusnidulans and a. fumigatus belong to this group. our objective was to determine the oxylipins formed from linoleic acid by a. clavatus ... | 2010 | 20045744 |
| structural and functional studies of aspergillus clavatus n(5)-carboxyaminoimidazole ribonucleotide synthetase . | n(5)-carboxyaminoimidazole ribonucleotide synthetase (n(5)-cair synthetase), a key enzyme in microbial de novo purine biosynthesis, catalyzes the conversion of aminoimidazole ribonucleotide (air) to n(5)-cair. to date, this enzyme has been observed only in microorganisms, and thus, it represents an ideal target for antimicrobial drug development. here we report the cloning, crystallization, and three-dimensional structural analysis of aspergillus clavatus n(5)-cair synthetase solved in the prese ... | 2010 | 20050602 |
| pathogenicity of the fungus, aspergillus clavatus, isolated from the locust, oedaleus senegalensis, against larvae of the mosquitoes aedes aegypti, anopheles gambiae and culex quinquefasciatus. | the use of insect pathogenic fungi is a promising alternative to chemical control against mosquitoes. among the hyphomycetes isolated from insects for mosquito control, the genus aspergillus remains the least studied. in september 2005, four fungi were isolated from the senegalese locust, oedaleus senegalensis kraus (orthoptera: acrididae), collected in dakar, senegal. one of these fungi, identified as aspergillus clavatus, desmazières (eurotiales: trichocomaceae) was highly pathogenic against l ... | 2009 | 20050773 |
| rna binding proteins mediate the ability of a fungus to adapt to the cold. | little is known about how fungi adapt to chilling. in eubacteria, cold shock proteins (csps) facilitate translation by destabilizing rna secondary structure. animals and plants have homologous cold shock domains within proteins, and additional glycine-rich rna binding proteins (grps), but their role in stress resistance is poorly understood. in this study, we identified grp homologues in diverse fungi. however, only aspergillus clavatus and metarhizium anisopliae possessed cold shock domains. bo ... | 2010 | 20050869 |
| functional characterization of the copper-transporting p-type atpase gene of penicillium janthinellum strain gxcr. | copper (cu)-transporting p-type atpase (ctpa) genes have been documented to play an important role in resistance to heavy metals. however, our understanding of roles of ctpa genes of the filamentous fungi was based only on sequence similarity prediction before. in a previous study, we isolated a penicillum janthinellum strain gxcr of higher tolerance to cu (200 mm). in this study, we cloned the partial cdna of ctpa gene, named pcpa, from the strain gxcr. sequence alignment indicated that the clo ... | 2010 | 20127468 |
| extracellular synthesis of silver bionanoparticles from aspergillus clavatus and its antimicrobial activity against mrsa and mrse. | new enzymatic approaches using bacteria and fungi for the synthesis of nanoparticles in both intra- and extracellular are playing an advanced key role in pharmacotherapeutics. in the present study we have reported on the use of fungus aspergillus clavatus for the extracellular synthesis of bionanoparticles from silver nitrate (agno(3)) solution. the bionanoscale particles were characterized by uv-visible spectroscopy, thin layer chromatography, atomic force microscopy (afm) and ftir. the synthes ... | 2010 | 20189360 |
| propionic acid production by cofermentation of lactobacillus buchneri and lactobacillus diolivorans in sourdough. | cooperative metabolism of lactobacilli in silage fermentation converts lactate to propionate. this study aimed to determine whether propionate production by lactobacillus buchneri and lactobacillus diolivorans can be applied for bread preservation. propionate formation was observed in cofermentation with l. buchneri and l. diolivorans in modified mrs broth as well as sourdough with low, medium and high ash contents. 48 mm of propionate was formed in sourdough with medium ash content, but only 9 ... | 2010 | 20227604 |
| the structure of clavatol, a metabolic product of aspergillus clavatus. | 1947 | 20253200 | |
| the action of culture filtrates of aspergillus clavatus on dermatophytes; in vitro experiments. | 1947 | 20262906 | |
| a highly thermostable antimicrobial peptide from aspergillus clavatus es1: biochemical and molecular characterization. | antimicrobial peptides (amps) are extremely attractive candidates as therapeutic agents due to their wide spectrum of antimicrobial activity and mechanism of action, which differs from that of small-molecule antibiotics. in this study, a 6.0-kda antimicrobial peptide from aspergillus clavatus es1, designated as acamp, was isolated by a one-step heat treatment. acamp was sensitive to proteolytic enzymes, stable between ph 5.0 and 10.0, and heat resistant (15 min at 100 degrees c). the acamp gene ... | 2010 | 20440534 |
| in vitro susceptibility of aspergillus spp. to dithiocarbamate organoruthenium compounds. | summary the in vitro antifungal activity of ruthenium dithiocarbamate compounds (1-5) was investigated and assessed for its activity against seven different species of aspergillus (aspergillus clavatus, aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus nomius, aspergillus tamarii and aspergillus terreus). analysis of in vitro susceptibility was performed using broth microdilution assay following the clinical and laboratory standards institute guidelines for filamentous fu ... | 2010 | 20557461 |
| the human ku autoantigen shares amino acid sequence homology with fungal, but not bacterial and viral, proteins. | context: molecular mimicry between autoantigens and microbial antigens is a possible triggering mechanism of autoimmunity. human ku is a dna-associated autoantigen targeted by autoantibodies in patients with systemic lupus erythematosus and related disorders; available data are consistent with a role of molecular mimicry in the pathogenesis of anti-ku autoimmunity, but no research exist on this topic. objective: we aimed to define the most probable microbial triggers of anti-ku autoimmunity via ... | 2011 | 20718578 |
| penicillium marneffei actin expression during phase transition, oxidative stress, and macrophage infection. | penicillium marneffei is an opportunistic fungal pathogen that exhibits thermally regulated dimorphism. at 25°c, this fungus grows vegetatively as mycelia, but at 37°c or upon invasion of a host, a fission yeast form is established. yet, despite increased numbers of molecular studies involving this fungus, the role of p. marneffei stress response-related proteins is not well characterized. actin is one of the proteins that have been proposed to play a role not only in cell transition, but also i ... | 2010 | 21088905 |
| antimycobacterial and antimicrobial study of new 1,2,4-triazoles with benzothiazoles. | in this study, we report the antimycobacterial and antimicrobial evaluation of newly synthesized 3-(3-pyridyl)-5-(4-methoxyphenyl)-4-(n-substituted-1,3-benzothiazol-2-amino)-4h-1,2,4-triazole 6a-j in good yields. all the synthesized compounds have been established by elemental analysis, ir, ¹h nmr, ¹³c-nmr and mass spectral data. in-vitro antimycobacterial activity was carried out against (mycobacterium tuberculosis) h₃₇rv strain using lowenstein-jensen medium and antimicrobial activity against ... | 2010 | 21110343 |
| [tremorgenic syndrome in a cattle herd after feeding silage contaminated with a. clavatus]. | the clinical signs, pathological and laboratory findings of cattle suffering from a tremorgenic syndrome are described. animals on a farm with a total of 22 cows, 18 heifers and 9 calves were fed mouldy grass and spent malt-grain silage. five heifers were affected with muscular tremor, hyperexcitability and hypersensitivity. they were ataxic or in sternal recumbency, while their appetite remained normal. haematology and blood chemistry in two heifers as well as cerebrospinal fluid from one sick ... | 2011 | 21360447 |
| permanent genetic resources added to molecular ecology resources database 1 october 2009-30 november 2009. | this article documents the addition of 411 microsatellite marker loci and 15 pairs of single nucleotide polymorphism (snp) sequencing primers to the molecular ecology resources database. loci were developed for the following species: acanthopagrus schlegeli, anopheles lesteri, aspergillus clavatus, aspergillus flavus, aspergillus fumigatus, aspergillus oryzae, aspergillus terreus, branchiostoma japonicum, branchiostoma belcheri, colias behrii, coryphopterus personatus, cynogolssus semilaevis, cy ... | 2010 | 21565039 |
| correction: biofabrication of anisotropic gold nanotriangles using extract of endophytic aspergillus clavatus as a dual functional reductant and stabilizer. | 2011 | 21711773 | |
| synthesis of 1,2,4-triazole derivatives containing benzothiazoles as pharmacologically active molecule. | in attempt to make significant pharmacologically active molecule, we report here the synthesis and in vitro antimicrobial and antitubercular activity of various series of 3-(3-pyridyl)-5-(4-nitrophenyl)-4-(n-substituted-1,3-benzothiazol-2-amino)-4h-1,2,4-triazole. the antimicrobial activity of title compounds were examined against two gram-positive bacteria (staphylococcus aureus, streptococcus pyogenes), two gram-negative bacteria (escherichia coli, pseudomonas aeruginosa), and three fungi (can ... | 2011 | 21714763 |
| a new class of 2-(4-cyanophenyl amino)-4-(6-bromo-4-quinolinyloxy)-6-piperazinyl (piperidinyl)-1,3,5-triazine analogues with antimicrobial/antimycobacterial activity. | this study presents the synthesis and in vitro pharmacological evaluations of novel 2-(4-cyanophenyl amino)-4-(6-bromo-4-quinolinyloxy)-6-piperazinyl (piperidinyl)-1,3,5-triazines. the title compounds were assayed for their in vitro antimicrobial activity against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria) and four fungi (aspergillus niger, aspergillus fumigatus, a ... | 2011 | 21740100 |
| isolation and characterization of neosartorya fischeri antifungal protein (nfap). | a novel 6.6kda antifungal peptide (nfap) from the culture supernatant of the mold, neosartorya fischeri (anamorf: aspergillus fischerianus), and its encoding gene were isolated in this study. nfap is a small, basic and cysteine-rich protein consisting of 57 amino acid residues. it shows 37.9-50% homology to similar proteins described in literature from aspergillus clavatus, aspergillus giganteus, aspergillus niger, and penicillium chrysogenum. the in silico presumed tertiary structure of nfap, e ... | 2011 | 21741420 |
| synthesis and studies of novel 2-(4-cyano-3-trifluoromethylphenyl amino)-4-(quinoline-4-yloxy)-6-(piperazinyl/piperidinyl)-s-triazines as potential antimicrobial, antimycobacterial and anticancer agents. | a series of novel s-triazine analogs were synthesized and characterized by ir, (1)h nmr, (13)c nmr, (19)f nmr spectroscopy and elemental analysis. preliminary screening of target compounds against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria), four fungi (aspergillus niger, aspergillus fumigatus, aspergillus clavatus, candida albicans) and mycobacterium tuberculosis ... | 2011 | 21794959 |
| composition and antifungal activities of the leaf essential oil of neolitsea parvigemma from taiwan. | the hydrodistillated leaf essential oil of neolitsea parvigemma was analyzed to determine its composition and yield. sixty-two compounds were identified, the main components being beta-caryophyllene (14.2%), beta-eudesmol (12.9%), alpha-cadinol (10.2%) and tau-cadinol (8.8%). oxygenated sesquiterpenes (48.9%) and sesquiterpene hydrocarbons (48.8%) were the predominant groups of compounds. the antifungal indexes of the leaf oil against the 7 fungi, aspergillus clavatus, a. niger, chaetomium globo ... | 2011 | 21941915 |
| Fluorinated s-triazinyl piperazines as antimicrobial agents. | A series of 1,3,5-triazine derivatives that contain 4-amino-2-trifluoromethyl-benzonitrile, 8-hydroxyquinoline, and different piperazines as substituents at the carbon atoms of the triazine ring have been synthesized by a simple and efficient synthetic protocol. The chemical structures of the compounds were elucidated with the aid of IR, 1H NMR and 13C NMR spectroscopy, and elemental analysis. The antimicrobial activity of the compounds was tested against seven bacteria (Staphylococcus aureus MT ... | 2011 | 21950158 |
| 1,3-Dihydro-2H-indol-2-ones derivatives: design, synthesis, in vitro antibacterial, antifungal and antitubercular study. | 1,3-dihydro-2H-indol-2-ones derivatives are reported to exhibit a wide variety of biodynamic activities such as antituberculer, anti HIV, fungicidal, antibacterial, anticonvulsant. These valid observations led us to synthesize some new indole-2-one derivative. Thus, herein we report synthesis of various 5-substituted-3-[{5-(6-methyl-2-oxo/thioxo-4-phenyl-1,2,3,4 tetrahydro pyrimidin-5-yl)-1,3,4-thiadiazol-2-yl}imino]-1,3-dihydro-2H-indol-2-one derivatives 4a-l using one pot multicomponent-Bigine ... | 2011 | 21981980 |
| identification and engineering of the cytochalasin gene cluster from aspergillus clavatus nrrl 1. | cytochalasins are a group of fungal secondary metabolites with diverse structures and bioactivities, including cytochalasin e produced by aspergillus clavatus, which is a potent anti-angiogenic agent. here, we report the identification and characterization of the cytochalasin gene cluster from a. clavatus nrrl 1. as a producer of cytochalasin e and k, the genome of a. clavatus was analyzed and the ∼30 kb ccs gene cluster was identified based on the presence of a polyketide synthase-nonribosomal ... | 2011 | 21983160 |
| in silico characterization of alkaline proteases from different species of aspergillus. | a total of 49 protein sequences of alkaline proteases retrieved from genbank representing different species of aspergillus have been characterized for various physiochemical properties, homology search, multiple sequence alignment, motif, and super family search and phylogenetic tree construction. the sequence level homology was obtained among different groups of alkaline protease enzymes, viz alkaline serine protease, oryzin, calpain-like protease, serine protease, subtilisin-like alkaline prot ... | 2011 | 22072140 |
| Biochemical characterization of indole prenyltransferases: Filling the last gap of prenylation positions by a 5-dimethylallyl tryptophansynthase from Aspergillus clavatus. | A putative prenyltransferase gene ACLA_031240 belonging to the dimethylallyltryptophan synthase superfamily was identified in the genome sequence of Aspergillus clavatus and overexpressed in E. coli. The soluble His-tagged protein EAW08391 was purified to near homogeneity and used for biochemical investigation with diverse aromatic substrates in the presence of different prenyl diphosphates. It has shown that in the presence of dimethylallyl diphosphate (DMAPP), the recombinant enzyme accepted v ... | 2011 | 22123822 |
| Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: An ethnomedicinal plant. | This study was carried out with an objective to investigate the antibacterial and antifungal potentials of leaves of Cassia fistula Linn. The aim of the study is to assess the antimicrobial activity and to determine the zone of inhibition of extracts on some bacterial and fungal strains. In the present study, the microbial activity of hydroalcohol extracts of leaves of Cassia fistula Linn. (an ethnomedicinal plant) was evaluated for potential antimicrobial activity against medically important ba ... | 2011 | 22171301 |
| synthesis and biological evaluation of some thiazolidinones as antimicrobial agents. | a novel series of thiazolidinone derivatives namely 4-(4-dimethylamino-6-{4-[5-(4-ethylpiperazin-1-ylmethyl)-4-oxo-2-phenylthiazolidin-3-yl]-phenylamino}-[1,3,5]triazin-2-yloxy)-1-methyl-1h-quinolin-2-one have been synthesized from the key intermediate 4-[4-(4-aminophenylamino)-6-dimethylamino-[1,3,5]triazin-2-yloxy]-1-methyl-1h-quinolin-2-one (7). condensation reaction of compound 7 with different aldehyde derivatives were performed to obtain schiff base derivatives, which after cyclization gav ... | 2011 | 22182927 |
| use of 18s ribosomal dna polymerase chain reaction-denaturing gradient gel electrophoresis to study composition of fungal community in 2 patients with intestinal transplants. | fungi form a diverse microbial community in the human intestine. little is known about the succession of species after intestinal transplantation. we investigated the alterations of the gut fungal population in 2 patients with intestinal allografts. the ileal effluent and feces were fingerprinted using denaturing gradient gel electrophoresis, with confirmation by dna sequencing. analysis of 18s ribosomal dna indicated that the phylogenetic diversity of the fungal communities was higher soon afte ... | 2012 | 22305239 |
| genomic characteristics comparisons of 12 food-related filamentous fungi in trna gene set, codon usage and amino acid composition. | filamentous fungi are widely exploited in food industry due to their abilities to secrete large amounts of enzymes and metabolites. the recent availability of fungal genome sequences has provided an opportunity to explore the genomic characteristics of these food-related filamentous fungi. in this paper, we selected 12 representative filamentous fungi in the areas of food processing and safety, which were aspergillus clavatus, a. flavus, a. fumigatus, a. nidulans, a. niger, a. oryzae, a. terreus ... | 2012 | 22305983 |
| synthesis of benzimidazolyl-1,3,4-oxadiazol-2ylthio-n-phenyl (benzothiazolyl) acetamides as antibacterial, antifungal and antituberculosis agents. | to affiliate multiple bioactivities in a compact heteronuclei, two series of benzimidazole based 1,3,4-oxadiazoles were synthesized and assessed in vitro for their efficacy as antimicrobial agents against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneri), four fungi (aspergillus niger, aspergillus fumigatus, aspergillus clavatus, candida albicans) and mycobacterium tuberc ... | 2012 | 22516426 |
| synthesis of s-triazine-based thiazolidinones as antimicrobial agents. | a novel series of thiazolidinone derivatives, namely 4-{4-dimethylamino-6-[4-oxo-2-phenyl-5-(4-pyridin-2-yl-piperazin-1-ylmethyl)-thiazolidin-3-yl]-[1,3,5]-triazin-2-yloxy}-1-methyl-1h-quinolin-2-ones, have been synthesized from the key intermediate 4-(4-amino-6-dimethylamino-[1,3,5]-triazin-2-yloxy)-1-methyl-1h-quinolin-2-one (5). compound 5 was condensed with various aldehydes to give schiff base derivatives, which after cyclization gave thiazolidinones that were linked with 1-pyridin-2-yl-pip ... | 2012 | 22624326 |
| mycobiota and mycotoxin contamination of maize flours and popcorn kernels for human consumption commercialized in spain. | mycobiota and co-occurrence of aflatoxins, citrinin, ochratoxin a and zearalenone in 30 samples of maize flours and 30 of popcorn kernels purchased in spain for human consumption were determined. the mycotoxin-producing ability of aspergillus, fusarium and penicillium spp. was also studied. total fungal counts of maize flours ranged from <10 to 8.4 × 10(4) cfu/g and predominant mycobiota belonged to aspergillus spp. and penicillium spp. in popcorn kernels samples the most frequent species were a ... | 2012 | 22850379 |
| in vitro antibacterial and antifungal activities of cassia fistula linn. fruit pulp extracts. | aim of the study is to assess the antimicrobial activity cassia fistula fruit pulp extracts on some bacterial and fungal strains. hydro alcohol and chloroform extracts of cassia fistula fruit pulp were evaluated for the potential antimicrobial activity. the antimicrobial activity was determined in both the extracts using the agar disc diffusion method. extracts were effective on tested microorganisms. the antibacterial and antifungal activities of solvent extracts (5, 25, 50, 100, 250 μg/ml) of ... | 2012 | 23049197 |
| synthesis and characterization of some new quinoline based derivatives endowed with broad spectrum antimicrobial potency. | the synthesis of a novel series of 2-(5-(2-chloro-6-fluoroquinolin-3-yl)-3-(aryl)-4,5-dihydro-1h-pyrazol-1-yl)thiazol-4(5h)-ones (4a-l) and n-(4-(2-chloro-6-fluoroquinolin-3-yl)-6-(aryl)pyrimidin-2-yl)-2-morpholinoacetamides (7a-l) are described in the present paper. the chemical structures of compounds have been elucidated by ir, (1)h nmr, (13)c nmr and mass spectral data. antimicrobial activity was measured against escherichia coli (mtcc 443), pseudomonas aeruginosa (mtcc 1688), staphylococcus ... | 2012 | 23058887 |
| spontaneous degenerative polioencephalomyelopathy in feeder pigs--a new motor neuron disease? | a central nervous disorder occurred spontaneously in a herd of feeder pigs characterized by muscle fasciculations, convulsions, squealing, and acute death in numerous animals. histopathology revealed a degenerative poliomyeloencephalopathy of brain stem and spinal cord consisting of neuronal hypertrophy, chromatolysis, neuronophagia, and satellitosis associated with wallerian degeneration of ventral rootlets and neurogenic muscle atrophy of limb musculature. the sudden onset of clinical signs an ... | 2012 | 23227771 |
| biomass-to-bio-products application of feruloyl esterase from aspergillus clavatus. | the structural polysaccharides contained in plant cell walls have been pointed to as a promising renewable alternative to petroleum and natural gas. ferulic acid is a ubiquitous component of plant polysaccharides, which is found in either monomeric or dimeric forms and is covalently linked to arabinosyl residues. ferulic acid has several commercial applications in food and pharmaceutical industries. the study herein introduces a novel feruloyl esterase from aspergillus clavatus (acfae). along wi ... | 2013 | 23229566 |
| identification of ophiobolin f synthase by a genome mining approach: a sesterterpene synthase from aspergillus clavatus. | during a screening of putative diterpene synthase genes found in public databases using the aspergillus oryzae expression system, it was found that a single transformant with the acla_76850 gene from a. clavatus produced a sesterterpene alcohol, ophiobolin f, and three minor sesterterpene hydrocarbons. the sesterterpene synthase has two catalytically independent domains (prenyltransferase/terpene cyclase) which are homologous to those of diterpene synthase, fusicoccadiene synthase. coevolution o ... | 2013 | 23324037 |
| design, synthesis, antimicrobial activity and anti-hiv activity evaluation of novel hybrid quinazoline-triazine derivatives. | a series of novel hybrid quinazoline-triazine derivatives was designed and synthesized from cyanuric chloride and anthranilic acid through sequential reactions, which contain different pharmacophores like quinazoline and substituted diaryl triazine (data) linked with ethylene diamine. all the newly synthesized compounds were characterized by infrared, (1)h-nmr, (13)c-nmr, ms and elemental analysis. further, we evaluated the in vitro anti-hiv activity of the newly synthesized compounds against hi ... | 2014 | 23327639 |
| novel cationic quinazolin-4(3h)-one conjugated fullerene nanoparticles as antimycobacterial and antimicrobial agents. | a series of novel cationic fullerene derivatives bearing a substituted-quinazolin-4(3h)-one moiety as a side arm were synthesized using the 1,3-dipolar cycloaddition reaction of c60 with azomethine ylides generated from the corresponding schiff bases of substituted quinazolinones. the synthesized compounds 5a-f were characterized by elemental analysis, ft-ir, 1h nmr, 13c nmr, and esi-ms and screened for their antibacterial activity against mycobacterium tuberculosis (h37rv) and antimicrobial act ... | 2013 | 23359525 |
| disparate proteome responses of pathogenic and nonpathogenic aspergilli to human serum measured by activity-based protein profiling (abpp). | aspergillus fumigatus is the primary pathogen causing the devastating pulmonary disease invasive aspergillosis in immunocompromised individuals. there is high genomic synteny between a. fumigatus and closely related rarely pathogenic neosartorya fischeri and aspergillus clavatus genomes. we applied activity-based protein profiling to compare unique or overexpressed activity-based probe-reactive proteins of all three fungi over time in minimal media growth and in response to human serum. we found ... | 2013 | 23599423 |
| synthetic tactics of new class of 4-aminothieno[2,3-d]pyrimidine-6-carbonitrile derivatives acting as antimicrobial agents. | thermal selective reactions were studied on oxothieno[2,3-d]pyrimidine-6-carboxamide 3 with pocl3 and pcl5. at 25-50 °c, the c7-amide rearranges to nitrile furnished compound 4 in 85-90% yield, while at 80-110 °c furnished mixture of products 4 and 5 in 28-68% yields. the chloro displacement with amines in compound 5 yielded 4-aminothieno[2,3-d]pyrimidine-6-carbonitrile derivatives 8(a-h) and 9(a-e). antimicrobial activity of new compounds was studied against several bacteria such as staphylococ ... | 2013 | 23644214 |
| synthesis, spectroscopic, molecular orbital calculation, cytotoxic, molecular docking of dna binding and dna cleavage studies of transition metal complexes with n-benzylidene-n'-salicylidene-1,1-diaminopropane. | eight mononuclear chromium(iii), manganese(ii), iron(iii), cobalt(ii), nickel(ii), copper(ii), zinc(ii) and cadmium(ii) complexes of schiff's base ligand were synthesized and determined by different physical techniques. the complexes are insoluble in common organic solvents but soluble in dmf and dmso. the measured molar conductance values in dmso indicate that the complexes are non-electrolytic in nature. all the eight metal complexes have been fully characterized with the help of elemental ana ... | 2013 | 23831943 |
| site-directed mutagenesis of catalytic residues in n(5)-carboxyaminoimidazole ribonucleotide synthetase. | n(5)-cair synthetase, an essential enzyme in microorganisms, converts 5-aminoimidazole ribonucleotide (air) and bicarbonate to n(5)-cair with the aid of atp. previous x-ray crystallographic analyses of aspergillus clavatus n(5)-cair synthetase postulated that r271, h273, and k353 were important for bicarbonate binding and for catalysis. as reported here, site-directed mutagenesis of these residues revealed that r271 and h273 are, indeed, critical for bicarbonate binding and catalysis whereas all ... | 2013 | 23899325 |
| isolation and characterization of antifungal compound from lactobacillus plantarum kcc-10 from forage silage with potential beneficial properties. | the purpose of this study was to isolate, identify and characterize an antifungal compound from lactobacillus plantarum kcc-10 from forage silage with potential beneficial properties. | 2013 | 23910250 |
| mycotoxicoses of ruminants and horses. | in the current study, mycotoxicoses of ruminants and horses are reviewed, with an emphasis on the occurrence of these diseases in south america. the main mycotoxicoses observed in grazing cattle include intoxications by indole-diterpenoid mycotoxins (paspalum spp. contaminated by claviceps paspali, lolium perenne infected by neotyphodium lolii, cynodon dactylon infected by claviceps cynodontis, and poa huecu), gangrenous ergotism and dysthermic syndrome (hyperthermia) caused by festuca arundinac ... | 2013 | 24091682 |
| small chemical chromatin effectors alter secondary metabolite production in aspergillus clavatus. | the filamentous fungus aspergillus clavatus is known to produce a variety of secondary metabolites (sm) such as patulin, pseurotin a, and cytochalasin e. in fungi, the production of most sm is strongly influenced by environmental factors and nutrients. furthermore, it has been shown that the regulation of sm gene clusters is largely based on modulation of a chromatin structure. communication between fungi and bacteria also triggers chromatin-based induction of silent sm gene clusters. consequent ... | 2013 | 24105402 |
| dysbiosis of gut fungal microbiota is associated with mucosal inflammation in crohn's disease. | we aim to characterize the fungal microbiota in the intestinal mucosa and feces in patients with crohn's disease (cd). | 2014 | 24275714 |
| two novel hepatocellular carcinoma cycle inhibitory cyclodepsipeptides from a hydrothermal vent crab-associated fungus aspergillus clavatus c2wu. | two novel cyclodepsipeptides containing an unusual anthranilic acid dimer and a d-phenyllactic acid residues, clavatustides a and b, were identified from cultured mycelia and broth of aspergillus clavatus c2wu isolated from xenograpsus testudinatus, which lives at extreme, toxic habitat around the sulphur-rich hydrothermal vents in taiwan kueishantao. this is the first example of cyclopeptides containing an anthranilic acid dimer in natural products, and the first report of microbial secondary m ... | 2013 | 24317468 |
| the gene patg involved in the biosynthesis pathway of patulin, a food-borne mycotoxin, encodes a 6-methylsalicylic acid decarboxylase. | patulin is a mycotoxin produced by fungal genera such as aspergillus, penicillium and byssochlamys. it induces neurological, gastrointestinal and immunological effects, which is why patulin belongs to a short list of mycotoxins whose level in food is regulated in many countries around the world. recently, a cluster gathering 15 genes involved in the biosynthesis of patulin has been identified in aspergillus clavatus, but so far, only 4 genes encoding 6-methylsalicylic acid synthase, m-cresol hyd ... | 2014 | 24334092 |
| ribonuclease production by free and immobilizedaspergillus clavatus cells. | several fungal strains ofaspergillus andpenicillium were immobilized by cryopolymerization in polyvinyl alcohol cryogel beads.aspergillus clavatus was the best producer of extracellular ribonuclease. enzyme productivity and growth of free and immobilized cells in shake flasks and agitated bioreactor were studied. ribonuclease production and growth behaviour depended on concentrations of glucose, peptone and soybean in the culture medium. enzyme production was influenced by agitation and aeration ... | 1993 | 24419834 |
| understanding the function of conserved variations in the catalytic loops of fungal glycoside hydrolase family 12. | enzymes that cleave the xyloglucan backbone at unbranched glucose residues have been identified in gh families 5, 7, 12, 16, 44, and 74. fungi produce enzymes that populate 20 of 22 families that are considered critical for plant biomass deconstruction. we searched for gh12-encoding genes in 27 eurotiomycetes genomes. after analyzing 50 gh12-related sequences, the conserved variations of the amino acid sequences were examined. compared to the endoglucanases, the endo-xyloglucanase-associated ysg ... | 2014 | 24578305 |
| biochemical characterization of the first fungal glycoside hydrolyase family 3 β-n-acetylglucosaminidase from rhizomucor miehei. | a novel β-n-acetylglucosaminidase gene (rmnag) from rhizomucor miehei was cloned and expressed in escherichia coli. rmnag shares the highest identity of 37% with a putative β-n-acetylglucosaminidase from aspergillus clavatus. the recombinant enzyme was purified to homogeneity. the optimal ph and temperature of rmnag were ph 6.5 and 50 °c, respectively. it was stable in the ph range 6.0-8.0 and at temperatures below 45 °c. rmnag exhibited strict substrate specificity for p-nitrophenyl β-n-acetylg ... | 2014 | 24811866 |
| zn-driven discovery of a hydrothermal vent fungal metabolite clavatustide c, and an experimental study of the anti-cancer mechanism of clavatustide b. | a naturally new cyclopeptide, clavatustide c, was produced as a stress metabolite in response to abiotic stress elicitation by one of the hydrothermal vent fluid components zn in the cultured mycelia of aspergillus clavatus c2wu, which were isolated from xenograpsus testudinatus. x. testudinatus lives at extreme, toxic habitat around the sulphur-rich hydrothermal vents in taiwan kueishantao. the known compound clavatustide b was also isolated and purified. this is the first example of a new hydr ... | 2014 | 24879544 |
| synthesis and biological evaluation of novel n-aryl maleimide derivatives clubbed with α-hydroxyphosphonates. | a series of novel molecules 5a-g containing n-aryl maleimide and α-hydroxyphosphonate moieties were synthesized. a distinct approach for high-yielding synthesis of α-hydroxyphosphonates has been discovered using various catalyst and solvents. the structures of the synthesized compounds were elucidated by ir, nmr, ms and chn analysis. all the synthesized compounds were tested for qualitative (zone of inhibition) and quantitative (mic) antimicrobial activities against two pathogenic bacteria such ... | 2014 | 24992076 |
| sequencing, physical organization and kinetic expression of the patulin biosynthetic gene cluster from penicillium expansum. | patulin is a polyketide-derived mycotoxin produced by numerous filamentous fungi. among them, penicillium expansum is by far the most problematic species. this fungus is a destructive phytopathogen capable of growing on fruit, provoking the blue mold decay of apples and producing significant amounts of patulin. the biosynthetic pathway of this mycotoxin is chemically well-characterized, but its genetic bases remain largely unknown with only few characterized genes in less economic relevant speci ... | 2014 | 25120234 |
| lymphocyte depletion after alemtuzumab induction disrupts intestinal fungal microbiota in cynomolgus monkeys. | the interactions of specific fungal phylotypes with immune cells have been recently documented; however, little is known whether gut fungal microbiota is influenced by aberrant immune response in immunosuppressive state. this study aimed to define the biologic impact of lymphocyte depletion on gut fungal microbiota and their relationship. | 2014 | 25136848 |
| bismuth(iii) volatilization and immobilization by filamentous fungus aspergillus clavatus during aerobic incubation. | as with many metals, bismuth can be accumulated or transformed by microorganisms. these interactions affect microbial consortia and bismuth environmental behaviour, mobility, and toxicity. recent research focused specifically on bismuth anaerobic transformation by bacteria and archaea has inspired the evaluation of the mutual interactions between bismuth and filamentous fungi as presented in this article. the aspergillus clavatus fungus proved resistant to adverse effects from bismuth contaminat ... | 2015 | 25367214 |
| multitoxin analysis of aspergillus clavatus-infected feed samples implicated in two outbreaks of neuromycotoxicosis in cattle in south africa. | aspergillus clavatus intoxication is a highly fatal neuromycotoxicosis of ruminants, especially cattle. it is caused by the ingestion of infected sprouting grain and sorghum beer residue. locomotor disturbances, tremors and paralysis are observed. histologically, degeneration and necrosis of larger neurons in the medulla oblongata, the midbrain, the thalamus and the ventral horns of the spinal cord are observed. although a range of mycotoxins such as patulin, cytochalasin e and pseurotin a have ... | 2014 | 25685962 |
| purification and biochemical characterization of an extracellular serine peptidase from aspergillus terreus. | peptidases are important because they play a central role in pharmaceutical, food, environmental, and other industrial processes. a serine peptidase from aspergillus terreus was isolated after two chromatography steps that showed a yield of 15.5%. its molecular mass was determined to be 43 kd, by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). this peptidase was active between ph 5.0 to 8.0 and had maximum activity at ph 7.0, at 45°c. when exposited with 1 m of urea, the en ... | 2016 | 25830777 |
| in-vitro antibacterial, antifungal, antioxidant and functional properties of bacillus amyloliquefaciens. | food born pathogenic bacteria and filamentous fungi are able to grow on most foods, including natural foods, processed foods, and fermented foods and create considerable economic loss. the aim of this study was to determine the antibacterial, antifungal, antioxidant and functional properties of bacillus amyloliquefaciens recovered from silage. | 2015 | 25858278 |
| aspergillus clavatus y2h0002 as a new endophytic fungal strain producing gibberellins isolated from nymphoides pe ltata in fresh water. | eighteen endophytic fungi with different colony morphologies were isolated from the roots of nymphoides peltata growing in the dalsung wetland. the fungal culture filtrates of the endophytic fungi were treated to waito-c rice seedling to evaluate their plant growth-promoting activities. culture filtrate of y2h0002 fungal strain promoted the growth of the waito-c rice seedlings. this strain was identified on the basis of sequences of the partial internal transcribed spacer region and the partial ... | 2015 | 25892921 |
| green synthesis and structural characterization of selenium nanoparticles and assessment of their antimicrobial property. | in the present study, selenium nanoparticles were biologically synthesized by non-pathogenic, economic and easy to handle bacterium ralstonia eutropha. the selenium oxo anion was reduced to selenium nanoparticles in the presence of the bacterium. the bacterium was grown aerobically in the reaction mixture. an extracellular, stable, uniform, spherical selenium nanoparticle was biosynthesized. the tem analysis revealed that the biosynthesized selenium nanoparticles were spherical in shape with siz ... | 2015 | 25972036 |
| diversity of food-borne bacillus volatile compounds and influence on fungal growth. | to evaluate the antifungal activity of the volatile organic compounds (vocs) produced by 75 different food-borne bacillus species against aspergillus niger, aspergillus flavus, aspergillus parasiticus, aspergillus clavatus, fusarium oxysporum f. sp. lactucae and moniliophthora perniciosa and to determine the vocs responsible for the inhibition. | 2015 | 25989039 |
| angiotensin i-converting enzyme (ace) inhibitory activities of sardinelle (sardinella aurita) by-products protein hydrolysates obtained by treatment with microbial and visceral fish serine proteases. | the angiotensin i-converting enzyme (ace) inhibitory activities of protein hydrolysates prepared from heads and viscera of sardinelle (sardinella aurita) by treatment with various proteases were investigated. protein hydrolysates were obtained by treatment with alcalase(®), chymotrypsin, crude enzyme preparations from bacillus licheniformis nh1 and aspergillus clavatus es1, and crude enzyme extract from sardine (sardina pilchardus) viscera. all hydrolysates exhibited inhibitory activity towards ... | 2008 | 26047434 |
| bivalent transition metal complexes of ono donor hydrazone ligand: synthesis, structural characterization and antimicrobial activity. | mononuclear transition metal complexes of mn(ii), co(ii), ni(ii), cu(ii), zn(ii) and cd(ii) with a new hydrazone ligand derived from pyrazine-2-carbohydrazide and 2-hydroxyacetophenone have been synthesized. the isolated complexes were characterized by elemental analysis, spectral and analytical methods including elemental analyses, ir, diffuse reflectance, (1)h-nmr, mass spectra, molar conductance, magnetic moment, esr, xrd, tg and sem analysis. from the elemental analyses data, the stoichiomet ... | 2015 | 26163785 |
| synthesis and in vitro antimicrobial evaluation of piperazine substituted quinazoline-based thiourea/thiazolidinone/chalcone hybrids. | in frames of the search for new biological entities to fight against recent drug-resistant microbial strains, we report a library of quinazoline-based thiourea/4-thiazolidinone/chalcone hybrids. the newly synthesized compounds were studied for efficacy against several bacteria (staphylococcus aureus, bacillus cereus, pseudomonas aeruginosa, and klebsiella pneumoniae) and fungi (candida albicans and aspergillus clavatus) using the broth dilution technique. from the biological evaluation, (e)-3-(3 ... | 2015 | 26165131 |
| pcr-rflp on β-tubulin gene for rapid identification of the most clinically important species of aspergillus. | aspergillus species are important agents of life-threatening infections in immunosuppressed patients. proper speciation in the aspergilli has been justified based on varied fungal virulence, clinical presentations, and antifungal resistance. accurate identification of aspergillus species usually relies on fungal dna sequencing but this requires expensive equipment that is not available in most clinical laboratories. we developed and validated a discriminative low-cost pcr-based test to discrimin ... | 2015 | 26264625 |
| sph3 is a glycoside hydrolase required for the biosynthesis of galactosaminogalactan in aspergillus fumigatus. | aspergillus fumigatus is the most virulent species within the aspergillus genus and causes invasive infections with high mortality rates. the exopolysaccharide galactosaminogalactan (gag) contributes to the virulence of a. fumigatus. a co-regulated five-gene cluster has been identified and proposed to encode the proteins required for gag biosynthesis. one of these genes, sph3, is predicted to encode a protein belonging to the spherulin 4 family, a protein family with no known function. construct ... | 2015 | 26342082 |
| the first case of total dystrophic onychomycosis caused by aspergillus clavatus resistant to antifungal drugs. | onychomycosis is a common fungal infection of nails which is mainly caused by dermatophyte species and less often by yeasts and non-dermatophyte molds. we present a case of onychomycosis due to aspergillus clavatus for the first time worldwide. the patient was an immunocompetent 32-year-old woman who identified with psoriasis of the nail. the presence of a. clavatus in a nail sample was confirmed using microscopic and culture analysis followed by pcr of the β-tubulin gene. after antifungal susce ... | 2016 | 26474550 |
| the trade-off of availability and growth inhibition through copper for the production of copper-dependent enzymes by pichia pastoris. | copper is an essential chemical element for life as it is a part of prosthetic groups of enzymes including super oxide dismutase and cytochrome c oxidase; however, it is also toxic at high concentrations. here, we present the trade-off of copper availability and growth inhibition of a common host used for copper-dependent protein production, pichia pastoris. | 2016 | 26897180 |
| histopathological effects of aspergillus clavatus (ascomycota: trichocomaceae) on larvae of the southern house mosquito, culex quinquefasciatus (diptera: culicidae). | aspergillus clavatus (ascomycota: trichocomaceae) was previously found to be an opportunistic pathogen of mosquitoes (diptera: culicidae). in the present study, the mechanism leading to its insecticidal activity was investigated regarding histological damages on culex quinquefasciatus larvae exposed to a. clavatus spores. multiple concentration assays using spore suspensions (0.5-2.5 × 10(8) spores ml(-1)) revealed 17.0-74.3 % corrected mortalities after 48 h exposure. heat-deactivated spores in ... | 2016 | 27020151 |
| phylogenetic and structural analysis of polyketide synthases in aspergilli. | polyketide synthases (pkss) of aspergillus species are multidomain and multifunctional megaenzymes that play an important role in the synthesis of diverse polyketide compounds. putative pks protein sequences from aspergillus species representing medically, agriculturally, and industrially important aspergillus species were chosen and screened for in silico studies. six candidate aspergillus species, aspergillus fumigatus af293, aspergillus flavus nrrl3357, aspergillus niger cbs 513.88, aspergill ... | 2016 | 27199544 |
| molecular basis of substrate recognition and specificity revealed in family 12 glycoside hydrolases. | fungal gh12 enzymes are classified as xyloglucanases when they specifically target xyloglucans, or promiscuous endoglucanases when they exhibit catalytic activity against xyloglucan and β-glucan chains. several structural and functional studies involving gh12 enzymes tried to explain the main patterns of xyloglucan activity, but what really determines xyloglucanase specificity remains elusive. here, three fungal gh12 enzymes from aspergillus clavatus (aclaxega), a. zonatus (aspzogh12), and a. te ... | 2016 | 27316782 |
| biofabrication of anisotropic gold nanotriangles using extract of endophytic aspergillus clavatus as a dual functional reductant and stabilizer. | biosynthesis of metal and semiconductor nanoparticles using microorganisms has emerged as a more eco-friendly, simpler and reproducible alternative to the chemical synthesis, allowing the generation of rare forms such as nanotriangles and prisms. here, we report the endophytic fungus aspergillus clavatus, isolated from surface sterilized stem tissues of azadirachta indica a. juss., when incubated with an aqueous solution of chloroaurate ions produces a diverse mixture of intracellular gold nanop ... | 2010 | 27502640 |
| linker flexibility facilitates module exchange in fungal hybrid pks-nrps engineering. | polyketide synthases (pkss) and nonribosomal peptide synthetases (nrpss) each give rise to a vast array of complex bioactive molecules with further complexity added by the existence of natural pks-nrps fusions. rational genetic engineering for the production of natural product derivatives is desirable for the purpose of incorporating new functionalities into pre-existing molecules, or for optimization of known bioactivities. we sought to expand the range of natural product diversity by combining ... | 2016 | 27551732 |
| synthesis and biological evaluation of newer 1,3,4-oxadiazoles incorporated with benzothiazepine and benzodiazepine moieties. | a series of thiazepines and diazepines having 1,3,4-oxadiazole moiety were synthesized, and they were analyzed for their in vitro antimicrobial activity against several bacteria (staphylococcus aureus, staphylococcus pyogenes, escherichia coli, and pseudomonas aeruginosa) and fungi (candida albicans, aspergillus niger, and aspergillus clavatus) and protozoa (entamoeba histolytica, giardia lamblia, trypanosoma cruzi and leishmania mexicana). few of the selected compounds were tested for their ant ... | 2017 | 28182579 |
| multitoxin analysis of <i>aspergillus clavatus</i>-infected feed samples implicated in two outbreaks of neuromycotoxicosis in cattle in south africa. | aspergillus clavatus intoxication is a highly fatal neuromycotoxicosis of ruminants, especially cattle. it is caused by the ingestion of infected sprouting grain and sorghum beer residue. locomotor disturbances, tremors and paralysis are observed. histologically, degeneration and necrosis of larger neurons in the medulla oblongata, the midbrain, the thalamus and the ventral horns of the spinal cord are observed. although a range of mycotoxins such as patulin, cytochalasin e and pseurotin a have ... | 2014 | 28235285 |
| microbial degradation of low-density polyethylene (ldpe) by aspergillus clavatus strain jask1 isolated from landfill soil. | polythene and plastic waste are found to accumulate in the environment, posing a major ecological threat. they are found to be considered non-degradable, once it enters the environment it has been found to remain there indefinitely. however, significant attention has been placed on biodegradable polymer, identification of microbes with degradative potential on plastic material. the aim of the present investigation was to biodegrade low-density polyethylene (ldpe) using potential fungi isolated f ... | 2016 | 28330123 |
| exploring the influence of domain architecture on the catalytic function of diterpene synthases. | terpenoid synthases catalyze isoprenoid cyclization reactions underlying the generation of more than 80,000 natural products. such dramatic chemodiversity belies the fact that these enzymes generally consist of only three domain folds designated as α, β, and γ. catalysis by class i terpenoid synthases occurs exclusively in the α domain, which is found with α, αα, αβ, and αβγ domain architectures. here, we explore the influence of domain architecture on catalysis by taxadiene synthase from taxus ... | 2017 | 28362483 |
| spectroscopic characterisation of two polyketide metabolites from cylindrocarpon sp. from driftwood. | two new polyketides, (5r,7r,9r)-7,9-dihydroxy-5-decanolide and (4e,6r,9r)- 6,9-dihydroxydec-4-enoic acid (2), were isolated from rice cultures of cylindrocarpon sp. sy-39 discovered during screening of driftwood at a shonai coast area in yamagata, japan. the structures of 1 and 2 were determined using a variety of spectroscopic methods. compound 2 exhibited moderate antimicrobial activity against staphylococcus aureus nbrc 13276 and aspergillus clavatus f 318a at a concentration of 50 μg per dis ... | 2017 | 28562088 |
| cloning and characterization of f3pyc gene encoding pyruvate carboxylase in aspergillus flavus strain (f3). | pyruvate carboxylase is a major enzyme for biosynthesis of organic acids like; citric acid, fumeric acid, and l-malic acid. these organic acids play very important role for biological remediation of heavy metals. in this study, gene walking method was used to clone and characterize pyruvate carboxylase gene (f3pyc) from heavy metal resistant indigenous fungal isolate aspergillus flavus (f3). 3579 bp of an open reading frame which encodes 1193 amino acid protein (isoelectric point: 6.10) with a c ... | 2017 | 28710744 |
| identification and antifungal activity of compounds from the mangrove endophytic fungus aspergillus clavatus r7. | two new coumarin derivatives, 4,4'-dimethoxy-5,5'-dimethyl-7,7'-oxydicoumarin (1), 7-(γ,γ-dimethylallyloxy)-5-methoxy-4-methylcoumarin (2), a new chromone derivative, (s)-5-hydroxy-2,6-dimethyl-4h-furo[3,4-g]benzopyran-4,8(6h)-dione (5), and a new sterone derivative, 24-hydroxylergosta-4,6,8(14),22-tetraen-3-one (6), along with two known bicoumarins, kotanin (3) and orlandin (4), were isolated from an endophytic fungus aspergillusclavatus (collection no. r7), isolated from the root of myoporum b ... | 2017 | 28825634 |