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impaired ketone body metabolism in the selenium deficient rat. possible implications.male rats were fed a selenium-deficient torula yeast diet with or without 0.2 ppm selenium (as sodium selenite) in the drinking water. selenium deficiency caused a significant increase of urinary acetoacetate excretion in fed rats, and 24 or 48 hours of starvation enhanced this effect. two days of selenium supplementation decreased the amount of urinary acetoacetate and 3-hydroxybutyrate to 50% of the deficiency value, indicating an enzymatic impairment in the selenium-deficient rat. no selenium ...19854058313
differential effects of dietary selenium on hepatic and renal glutathione metabolism in the rat.studies were undertaken to determine whether or not dietary selenium (se), as sodium selenite, at suboptimal (unsupplemented torula yeast diets, 0.02 ppm se) or supplemented up to dietary excess, but nontoxic, levels (5.0 ppm se) could selectively modify hepatic and renal reduced glutathione (gsh) levels and the enzymes involved in gsh metabolism. male rats were provided torula yeast semipurified diets containing approximately 0.02 ppm se in the basal diet and supplements of 0.1, 2.0, or 5.0 ppm ...19854064933
glucose-6-phosphate dehydrogenase. a transferred nuclear overhauser enhancement study of nadp+ conformations in enzyme-coenzyme binary complexes.the conformation of nadp+ in glucose-6-phosphate-dehydrogenase--nadp+ binary complexes has been investigated using proton-proton transferred nuclear overhauser enhancement measurements to determine interproton distance ratios between bound nadp+ protons. the enzymes from saccharomyces cerevisiae (brewer's yeast and baker's yeast) and hansenula jadinii (candida utilis, torula utilis) form binary complexes with nadp+ in which the glycosidic bond of the adenine moiety is in the anti conformation wh ...19846499847
effect of dietary composition and estradiol implants on hepatic microsomal mixed function oxidase and lipid deposition in growing chicks.three experiments were conducted to investigate the effect of diet and estradiol (e2) administration on hepatic microsomal mixed function oxidase (mfo) activity, e2 metabolism, and liver lipid content in male broiler chicks. broiler chicks (3 weeks of age) were fed either a corn-soybean (cs) diet or a diet containing fish meal, alfalfa meal, and torula yeast (fay) for 19 days in experiments 1 and 3 and for 14 days in experiment 2, respectively. half of the chicks were implanted with tubes contai ...19846514666
dietary induction of mulberry heart disease and hepatosis dietetica in pigs. i. nutritional aspects.two trials were conducted, involving 48 yorkshire specific pathogen-free pigs, three to four weeks old, to investigate diets which would result in a high incidence of deaths from mulberry heart disease and hepatosis dietetica in pigs. diets based on ground corn with torula yeast resulted in a high incidence of death. protein supplements of dried skim milk or soybean meal with corn did not induce a high incidence of death. diets supplemented with torula yeast had the lowest selenium concentration ...19724263917
effect of supplemental vitamin e and selenium in high moisture corn diets on the incidence of mulberry heart disease and hepatosis dietetica in pigs.forty-eight intact male pigs were used to investigate the influence of source of protein supplement, corn moisture content, and supplemental vitamin e-selenium survived the cidence of mulberry heart disease, hepatosis dietetica and associated lesions. pigs fed soybean meal-high moisture corn diets survived fewer days than pigs fed the other diets. none of the pigs fed torula yeast-dry corn or soybean meal-high moisture corn without supplemental vitamin e-selenium survived the 13 week trial. supp ...19724263921
interactions of selenium deficiency, vitamin e, polyunsaturated fat, and saturated fat on azoxymethane-induced colon carcinogenesis in male f344 rats.the effect of the interaction of selenium deficiency, excess vitamin e, and type of fat on colon carcinogenesis induced by azoxymethane (aom) was studied in male f344 rats. the experimental diets, based on a torula yeast diet and containing 20% stripped corn oil or 20% stripped lard, were as follows: 1) selenium deficient with adequate (50 mg/kg diet) vitamin e, 2) selenium deficient with excess (750 mg/kg diet) vitamin e, 3) selenium adequate with adequate vitamin e, and 4) selenium adequate wi ...19863458951
nucleotide sequence of tyrosine transfer rna from torulopsis utilis. 19694308683
vitamin e, selenium and methionine supplementation of dystrophogenic diets for pigs.forty-eight weanling s.p.f. yorshire pigs were used to study the influence of supplemental vitamin e (25 iu per kg of diet) selenium (0.5 ppm in diet) and methionine (0.1% in diet) on the incidence of hepatosis dietetica and mulberry heart disease when fed a torula yeast-corn diet. vitamin e and/or selenium increased pig survival. supplemental selenium resulted in increased liver selenium concentrations. no hepatosis dietetica was observed in any of the pigs. the addition of vitamin e and/or sel ...19724263922
[stimulation of growth in torulopsis utilis (candida utilis) by various vitamin- and growth-factor sources in solutions containing sucrose]. 19744471907
effect of supplemental selenium on pancreatic function and nutrient digestibility in the pig.the effect of se deficiency on pancreatic digestive enzyme activity and nutrient digestibility was studied by using pigs weaned at 3 wk of age from sows fed a diet deficient in se and low in vitamin e. pigs were fed a torula yeast diet supplemented with 100 iu dl-alpha-tocopheryl acetate/kg of diet. treatments were levels of supplemental se of 0, .025, .050, .075 or .100 ppm. apparent digestibility was determined at the end of the second and fourth week. digestive enzyme activity, glutathione pe ...19846608513
factors influencing the antitumorigenic properties of selenium in mice.sodium selenite (na2seo3) was administered at 2.0 micrograms selenium (se) to swiss icr mice six times over a 9-day period by intraperitoneal (i.p.) injection or by gastric gavage. survival time was significantly increased in ehrlich ascites tumor (eat)-bearing mice by 170 and 20%, respectively, compared to controls. in two separate studies, 5.0 micrograms se as na2seo3 or selenodiglutathione (gssesg) administered i.p. was more effective in inhibiting eat propagation in mice than either untreate ...19836631533
selenium retention in tissues and sperm of rats fed a torula yeast diet. 19734682443
[effect of torula utilis and candida arborea on the respiratory tract in workers employed in the production of yeast]. 19655853137
structure of the 5-s ribosomal rna gene and its adjacent regions in torulopsis utilis.a dna segment spanning one repeating unit of ribosomal rna(rrna) genes in a yeast strain, torulopsis utilis, has been cloned on a bacterial plasmid pbr322. the size of the cloned segment was about mr = 8.0 x 10(6). all of the genes for the four species of rrna were linked on it, and there was a long spacer between the 5-s and 18-s rrna coding regions. the nucleotide sequences of the regions flanking the 5-s rrna gene were determined and compared with those in the corresponding regions of sacharo ...19806254757
nucleotide sequences of the 5s ribosomal rna genes and their adjacent regions in schizosaccharomyces pombe.the organization of 5s ribosomal rna (rrna) genes in the genome of schizosaccharomyces pombe has been investigated by restriction and hybridization analyses. the 5s rrna genes were not linked to the other three species of rrna genes which formed a repeating unit of 6.9 megadaltons, but located in other regions surrounded by heterogeneous sequences. the 5s rrna gene organization in s. pombe is therefore different from those in other yeasts; saccharomyces cerevisiae and torulopsis utilis. four res ...19816275361
bioavailability to rats of selenium in various tuna and wheat products.bioavailability of selenium (se) in tuna and wheat at various stages of processing was studied in rats. the protein source of the rat diets was torula yeast with se supplied by either raw, precooked or canned tuna, or whole wheat flour, whole wheat bread or bran. sodium selenite was used as the standard. each se source was fed at three levels: 0.05, 0.10 and 0.15 ppm. by using increase in glutathione peroxidase (gsh-px) activity in liver, kidney and whole blood as an indicator of bioavailability ...19836296340
responses in calcium and phosphorus metabolism and hepatic lipid deposition among estrogenized chicks fed various dietary ingredients.the purpose of this study was to determine whether diet composition would influence calcium and phosphorus metabolism in chicks administered estrogen. at 1 day of age, broiler chicks were fed either a corn-soybean meal diet (cs), or an isoenergetic and isonitrogenous diet containing 5% fish meal, 5% alfalfa meal and 10% torula yeast (fay). at 21 days equivalent numbers were implanted with one of two lengths of silastic tubing containing estradiol dipropionate, while the remaining birds served as ...19846699740
effect of selenium on performance, serum selenium concentration and glutathione peroxidase activity in pigs.pigs from sows fed a diet deficient in se and low in vitamin e were fed a torula yeast diet supplemented with 100 iu dl-alpha-tocopheryl acetate/kg of diet. dietary treatments were levels of supplemental se of 0, .025, .050, .075 or .100 ppm. some death loss occurred in pigs receiving no supplemental se at approximately 5 wk of age. autopsy revealed liver and heart lesions typical of vitamin e-se deficiency. selenium supplement had no significant effect on average daily gain, feed intake or gain ...19846706871
diet composition, environmental temperature, and exogenous estradiol effects on hepatic lipid deposition in growing chicks.the interrelationships among dietary composition, environmental temperature, and level of estradiol (e2) administration on hepatic lipid deposition in chicks were studied. two levels of e2 were injected at three intervals over a 4-day period, in 3-week-old male leghorn chicks fed either a corn-soybean meal (cs) diet or a diet containing fish meal (fm) after 2 days fasting. the chicks were subjected to temperature ranges of 15 to 24 c (low) or 24 to 35 c (high) at 2 weeks of age. the e2 (in silas ...19846718303
sequence of trna ile iau from brewer's yeast.the nucleotide sequence of trnaile from brewer's yeast saccharomyces cerevisiae was determined. its primary structure is pg-g-u-c-u-c-u-u-m1g-m2g- c-c-c-a-g-d-d-g-g-d-d-a-a-g-g-c-a-c-c-g-u-g-c-u-i-a-u-t6 a-a-c-g-c-g-g-g-ga-d-m5 c-a-g-c-g-g-t-psi-c-g-m1 a-u-c-c-c-g-c-u-a-g-a-g-a-c-c-a-c-c-a. its anticodon is i-a-u. it should therefore recognize the three isoleucine codons and is for this reason probably the only isoacceptor trna for isoleucine in brewer's yeast. it presents a large homology with ...19846370253
the detection of delta-aminolaevulinic acid synthetase in anaerobically grown torulopsis utilis. 19724542156
effects of dietary selenium and vitamin e on hepatic mixed-function oxidase activities and in vivo covalent binding of aflatoxin b1 in rats.male weanling fischer-344 rats were fed a selenium (se)-vitamin e (ve) deficient torula yeast basal diet or that diet supplemented with a graded levels of se (0.2-6.0 ppm as na2seo3) or ve (100 iu/kg as all-rac-2-tocopheryl acetate), or both, for 4 or 6 weeks. se deficiency and excess (6.0 ppm) markedly depressed in vivo covalent binding of aflatoxin (afb1) to macromolecules in livers of rats killed 2 hours after an i.p. dose of 1 mg/kg tritiated afb1. ve supplementation had no effect. prior phe ...19826799626
effects of chlorinated bisphenols on torula yeast glucose-6-phosphate dehydrogenase.chlorinated bisphenol antibacterial and antifungal agents are potent inhibitors of torula yeast glucose-6-phosphate dehydrogenase (g6pd). several compounds were tested, including hexachlorophene [hcp; 2,2'-methylenebis(3,4,6-trichlorophenol)]; 2,2'-oxybis(tetrachlorophenol); 2',4-dihydroxy-2,3,3',5,5',6-hexachlorodiphenylmethane; 2,2'-methylenebis(3,4-dichlorophenol) (3,4-tcp); bithionol [2,2'-thiobis(4,6-dichlorophenol)]; 2,2'-methylenebis(3,5-dichlorophenol); 2,2'-dihydroxy-3,3',5,6,6'-pentach ...19816802984
effect of short-term feeding of sodium selenite on 7,12-dimethylbenz(a)anthracene-induced mammary carcinogenesis in the rat.the inhibitory activity of short-term feeding of one of four concentrations of dietary selenium against the induction of mammary gland carcinomas by 7,12-dimethylbenz(a)anthracene (dmba) was studied in female sprague-dawley rats. when 28 days old, the animals were placed on a torula yeast diet formulation which contained, by analysis, either 0.05, 0.15, 1.05, or 2.06 microgram of selenium, as sodium selenite, per g of diet. mammary cancer was induced by a single p.o. administration of either 7.5 ...19826814747
roles of selenium and sulfur-containing amino acids in protection against oxygen toxicity.tolerance and adaptation to hyperoxia have been correlated with increases in antioxidant enzymes. this study evaluated whether selenium deficiency would prevent an increase in glutathione peroxidase (gshpx), a selenium-containing enzyme, during oxygen exposure, and, thus, inhibit adaptation. because the torula yeast-based diet, which was used to produce selenium deficiency, was also deficient in cysteine and methionine, the effects of these deficiencies were also evaluated. when rats were expose ...19836876743
toxic effects of dietary selenium in the syrian hamster.selenium (se) toxicity and utilization was evaluated in hamsters fed casein- and torula yeast-based diets. 4-week-old hamsters received semipurified diets for 21 days. in experiment i diets were supplemented with either 0.25, 10, 20, 40 or 80 ppm se as sodium selenite (ss) and in experiment ii diets were supplemented with 0.1, 5.0 or 10.0 ppm as ss or selenomethionine (sm). blood and tissue se concentrations and glutathione peroxidase (gsh-px) activity were measured at the termination of the fee ...19836881915
beneficial effect of chromium-rich yeast on glucose tolerance and blood lipids in elderly subjects.twenty-four volunteers, mean age 78, including eight mildly non-insulin-dependent diabetics, were randomly allocated to one of two groups and were fed (daily for 8 wk) 9 g of either chromium-rich brewers' yeast (experimental) or chromium-poor torula yeast (control). before and after yeast supplementation, the serum glucose and insulin response to 100 g oral glucose was measured at 30 min intervals for 2 h. fasting serum cholesterol, total lipids, and triglycerides were also determined. in the to ...19807000589
interaction of initiator met-trnarmet (escherichia coli) and gly-trnaigly (staphylococcus epidermidis) with bacterial elongation factor tu:gtp complex.jekowsky et al. reported recently that elongation factor tu:gtp complex from escherichia coli protected aminoacyl-trna from digestion by pancreatic rnase (i). on the basis of their finding, we have developed the "rnase-resistance assay" for determination of the dissociation constant of aminoacyl-trna from aminoacyl-trna:ef-tu:gtp complex. by the use of this sensitive assay, the dissociation constants were estimated to be 3.6 x 10(-7) m for ala-trna1ala (torulopsis utilis), 7.9 x 10(-8) m for phe ...19817024261
effect of estrogen implants on hepatic lipid deposition in chicks fed different isonitrogenous and isocaloric diets.two experiments were conducted to determine if differences in response of livers to estradiol administration in male broiler chicks would be observed if fed isonitrogenous and isocaloric diets of different composition. a polymethylsiloxane tube filled with estradiol dipropionate was implanted under the neck skin to ensure a constant release of hormone. different tube sizes were implanted in two- to three-week-old chicks fed a basal corn-soybean meal (cs) diet or a diet containing fish meal, alfa ...19827054466
plasma estradiol, thyroid hormones, and liver lipid content in laying hens fed different isocaloric diets.two experiments were conducted with laying hens fed a corn-soy basal diet or diets containing fish meal, distillers dried grains with solubles (ddgs) or torula yeast formulated to be isocaloric and isonitrogenous with the basal diet. one half of the hens were kept at a temperature range of 13-24 degrees and the other half at 24-35 degrees for 49 days in experiment 2. liver lipid content was significantly lower in hens fed ddgs (experiment 1) and ddgs or fish meal (experiment 2) than in hens fed ...19827057268
some metabolic interrelationships between toxic levels of cadmium and nontoxic levels of selenium fed to rats.male rats were fed vitamin e-adequate, torula yeast-based diets for 30 days to assess the influence of dietary selenium (0, 0.1, or 1.0 ppm) on the toxicity of dietary cadmium (0, 30, or 60 ppm). at all selenium levels, increased cadmium intake depressed feed consumption, reduced feed efficiency and lowered body weight gain. in liver, concentrations of cadmium and zinc increased, and iron concentration decreased with increased intake of cadmium. dietary selenium did not affect concentrations of ...19827077426
comparative effects of sodium selenite and selenomethionine upon nutritional muscular dystrophy, selenium-dependent glutathione peroxidase, and tissue selenium concentrations of turkey poults.day-old poults from hens depleted of se were fed low-se basal diets (containing corn, soybean meal, and torula yeast but no added vitamin e) with graded levels of se supplied by na2seo3 or seleno-dl-methionine for 28 and 35 days in experiments 1 and 2, respectively. adding .04 ppm se to the basal diet significantly increased body weight and reduced both the incidence of gizzard myopathy and plasma glutamic-oxaloacetic transaminase (pgot) activity. further plasma se and se-dependent glutathione p ...19827088800
selenium in the testis of the rat: studies on its regulation and its importance for the organism.in male rats, fed for 10 weeks on a torula yeast-based, low selenium and low vitamin e diet, the selenium level and the glutathione peroxidase activity in the blood and in several tissues decreased by 50 to 98% compared with animals that received the same basal diet supplemented with 0.25 mg se/kg sodium selenite. in the testes, however, the selenium content did not differ from that of the control animals. despite the low selenium levels in the extragonadal tissues and their increased requiremen ...19827108639
nutritional availability to rats of selenium in tuna, beef kidney, and wheat.weanling male rats were fed a selenium (se)-deficient 40% torula yeast diet for 4 weeks and were then continued on depletion for another 4 weeks or were repleted with 0.1, 0.2, or 0.3 ppm se as sodium selenite or 0.2 ppm se as tuna, beef kidney, or high-se wheat. there were no significant differences in young red blood cell se, unfractionated red blood cell se, or liver se levels in groups fed diets containing 0.2 ppm se. glutathione peroxidase (gsh-px) activity in young red blood cells was lowe ...19817310542
selenium and reproductive function in boars fed a low selenium diet.a study was conducted with 24 crossbred boars (77.5 +/- 2.8 days of age) to determine the effects of low se status on various spermatozoal characteristics and on se concentration in semen, serum and primary and accessory reproductive tissues. all boars were fed a low se diet (cornstarch and torula yeast) ad libitum. twelve boars were injected every 14 +/- 1 days with sodium selenite (.33 mg se/kg body weight) and 12 served as saline-treated controls (low se status). at 210 +/- 5 days of age, six ...19817319974
effect of acriflavine on the hexokinase isozyme pattern of a yeast, hansenula jadinii.dried cells of a yeast, hansenula jadinii, that had been cultured aerobically with acriflavine, contained three hexokinase isozymes and metabolized glucose at 0.6 m to produce atp to phosphorylate nucleotides in the presence of a high concentration of phosphate. dried cells cultured aerobically without acriflavine contained two hexokinase isozymes and could not metabolize glucose under the same conditions. two of the isozymes of the yeast cultured with acriflavine were similar to isozymes of the ...19807388034
respiratory pentane: a measure of in vivo lipid peroxidation applied to rats fed diets varying in polyunsaturated fats, vitamin e, and selenium and exposed to nitrogen dioxide.pentane is one decomposition product of omega 6-unsaturated lipid hydroperoxides. the measurement of respiratory pentane is one of the most sensitive in vivo tests of lipid peroxidation. this measurement was applied to test the ability of a low level, short-term exposure of rats of nitrogen dioxide to induce lipid peroxidation. when exposed to 4.48 ppm nitrogen dioxide for 60 min, no increase in pentane production was detected. for 10 weeks prior to exposure, the rats were fed torula yeast-based ...19807423553
dietary selenium and age-related susceptibility of rat erythrocytes to oxidative damage.the effects of age and dietary selenium on cellular susceptibility to oxidative stress were studied in rat erythrocytes. young (26 or 40 days) and mature adult (11 or 15 months) male rats were fed a torula yeast-based low selenium and low vitamin e diet supplemented with either 0, 1.0 to 2.0 ppm selenium (as sodium selenite) for 5 weeks. the rates of spontaneous hemolysis amd methemoglobin formation and levels of glutathione (gsh), glucose-6-phosphate (g6p) dehydrogenase, but not of gsh peroxida ...19807441374
effect of dietary methionine on the biopotency of selenite and selenomethionine in the rat.the effect of dietary methionine (met) on the biopotency of selenium (se) from selenite and selenomethionine (se-met) was studied in rats fed a 30% torula yeast-based diet containing 0.24% met and less than 0.02 ppm se. biopotency was quantitated by assaying liver, plasma and heart for the glutathione peroxidase (gsh-px) increase elicited by a given level of se fed for 1 week to se-deficient rats. at dietary se levels up to 0.5 ppm se, the level of dietary met supplementation did not alter selen ...19817452376
effect of ingestion of 6-propyl-2-thiouracil on biochemical indices of selenium status in rats.biochemical indices of selenium (se) deficiency (liver se content and glutathione peroxidase (gshpx) activity, and urinary ketone bodies during fasting) were measured in se-deficient or se-sufficient control rats with or without ingestion of 6-propyl-2-thiouracil (ptu). male weanling wistar rats (50 to 60 g) were fed on a torula yeast-based se-deficient diet (se content, < 0.01 microgram/g), or on the same diet supplemented with sodium selenite (0.1 microgram se/g). the rats were given 0.05% ptu ...19957595971
mineral status in selenium-deficient rats compared to selenium-sufficient rats fed vitamin-free casein-based or torula yeast-based diet.to clarify the mineral status in selenium (se)-deficient rats fed a vitamin-free casein (vfc)-based or torula yeast (ty)-based diet, 24 weanling male wistar rats were divided into 4 groups fed diets using vfc or ty as the protein source and containing se at sufficient (0.5 microgram/g, +se) or deficient (0.019 microgram/g for vfc-based and < 0.005 microgram/g for ty-based diets, -se) level for 8 wk. ty supplied a larger amount of extra minerals (na, k, ca, mg, fe, mn, zn, and cu) except se than ...19937688535
selenium deficiency as a cause of overload of iron and unbalanced distribution of other minerals.previous studies have shown that there are hematological abnormalities in selenium (se)-deficient animals. this study examined the effects of se deficiency on various minerals in serum and other tissues of male wistar rats. the animals were given free access to either torula yeast-based se-deficient (sed) diet or se-adequate (sea) (containing 0.1 mg se/kg diet as sodium selenite) diet. blood was sampled after 12 and 24 weeks, and the rats were killed after 24 weeks, for the analysis of minerals ...19957766029
selenium from beef is highly bioavailable as assessed by liver glutathione peroxidase (ec 1.11.1.9) activity and tissue selenium.the bioavailability of se from ground beef has been previously found in this laboratory to be greater than that of selenite or selenate when fed to female fischer 344 rats. in the present study we examined the bioavailability of se from various commercial portions of beef, the liver, striploin, round, shoulder and brisket. all beef was cooked, freeze-dried, finely powdered and mixed with the other dietary ingredients. the experimental diets were fed to the weanling fischer 344 rats which had bee ...19947827008
selenium deficiency alters thyroid hormone metabolism in guinea pigs.in guinea pigs, activity of glutathione peroxidase in most organs is markedly lower than in organs of other rodents despite comparable dietary intakes and tissue levels of selenium. to determine if metabolism of selenium with respect to other selenoproteins also differs in guinea pigs, we measured the effects of selenium intake on thyroid hormone metabolism. weanling male hartley albino guinea pigs were fed a selenium-deficient torula yeast-based diet, or the same diet supplemented with 0.5 mg s ...19957861257
liver response to diet and estrogen in white leghorn and rhode island red chickens.experiments were conducted to compare the response of rhode island reds (rir) and white leghorns (wl) to estradiol (e2) administration and diets of different composition. in growing chickens injected with e2 for 7 days, rir accumulated significantly more hepatic lipid and had significantly less plasma lipid than wl. both rir and wl, injected for 4 days with e2, had lower hepatic lipids when fed a diet containing fish meal, alfalfa meal, and torula yeast (fay) than when fed a corn-soybean meal di ...19854039822
effect of fractions of fish meal and hepatic lipid deposition in estrogenized chicks.three experiments were conducted for the purpose of determining the effect of feeding isoenergetic diets with and without fish meal or its fractions on hepatic lipid deposition in estrogenized chicks. the chicks were fasted for 48 hr before being fed the experimental diets and were injected with estradiol three times during the experiment. after 4 days, hepatic lipid content was determined. in the first experiment, the quantity of hepatic lipid deposited per unit of liver and per 100 g body weig ...19846728789
tolerance of diets deficient or excessive in selenium by syrian hamsters.syrian hamsters were fed torula yeast (ty) diets with 8 selenium (se) supplement levels (0.0-10.0 ppm se as sodium selenite) or casein (c) diets with 5 supplement levels (0.0-5.0 ppm se as sodium selenite) for 25 weeks. whole blood se, plasma glutathione peroxidase (gsh-px) activity and erythrocyte gsh-px activity were measured after 5, 10, 15 and 25 weeks. at 25 weeks hematology was examined and tissue samples analyzed for se and evaluated for histopathological lesions. while survival was not i ...19836847126
effects of dietary selenium on uvb-induced skin carcinogenesis and epidermal antioxidant status.low plasma selenium levels have been linked to increased risk of non-melanoma skin cancer in humans. the present study examined the relationship between selenium level in the diet and development of skin tumors induced by ultraviolet radiation in female skh:hr-1 hairless mice. animals were maintained on a torula yeast-based diet containing either 0, 0.1, or 0.5 mg/kg selenium as na2seo3. ultraviolet light at a dose of 90 mj/cm2, three times weekly for 20 weeks, resulted in skin tumors in all gro ...19948176260
resolutive candida utilis fungemia in a nonneutropenic patient.we report here the second case of candida utilis infection in humans. the patient was apparently immunocompetent, had no central catheter, and survived an 8-day fungemia. genomic analysis confirmed the conspecificity of medical and industrial strains of c. utilis and that of the anamorphic yeast c. utilis with the teleomorphic yeast pichia jadinii.19938315009
effects of selenium deficiency on xenobiotic-metabolizing and other enzymes in rat liver.the present study was undertaken to characterize effects of selenium (se) deficiency on 16 enzymes recovered in either one or more of the subcellular fractions of rat liver (as a basis for future studies on the mechanisms underlying the observed changes). male rats were fed a torula-yeast based diet with 0.23 mg se/kg or the same diet with 0.009 mg se/kg, from weaning and for 10 weeks. statistically significant effects of se deficiency were the following: se-dependent glutathione peroxidase decr ...19938320056
glucose-6-phosphate dehydrogenase. structure-function relationships and the pichia jadinii enzyme structure.the primary structure of glucose-6-phosphate dehydrogenase from the yeast pichia jadinii (formerly candida utilis) has been determined. it consists of a 495-residue, n-terminally acetylated protein chain. the structure shows extensive differences from those of the corresponding mammalian, fruit fly, and bacterial enzymes (52-68% residue non-identities), but also from that of another yeast, saccharomyces cerevisiae (38%). a eubacterial type and a yeast type of glucose-6-phosphate dehydrogenase ar ...19938444164
selenium requirements of rats for normal hepatic and thyroidal 5'-deiodinase (type i) activities.the nutritional requirement of selenium for type i 5'-deiodinase activity in thyroid compared with liver was assessed in rats. male weanling sprague-dawley rats were fed a torula yeast-based diet for 20 wk. one group of rats was fed the se-deficient basal diet (0.01 mg se/kg). the other three groups were fed the basal diet plus sodium selenite at 0.05, 0.1 and 0.5 mg se/kg diet. liver 5'-deiodinase and glutathione peroxidase (gsh-px) activities were depressed in the group fed the se-deficient (b ...19938505673
[effect of ethoxyquin on the utilization of selenium in growing pigs].in a 2 x 3 factorial arrangement with 30 male castrates (german landrace; 13.7 +/- 1.7 kg b.w.), after a 2 week feeding period with a maize/torula yeast basal diet poor in selenium and without vitamin e supplementation, the effect of two ethoxiquin concentrations (0 and 150 mg/kg) und 3 selenium concentrations (0.075, 0.10 and 0.125 mg/kg) on growth, the activity of the se dependent gsh-px in the erythrocytes and the se concentrations in liver, kidney, heart and diaphragm was measured after 4 we ...19958526734
histological study of iron deposits in selenium-deficient rats.previous studies have shown that selenium (se) deficiency is associated with hematological abnormalities, which may result in an increased distribution of iron in various tissues. this report describes histological studies of the location of excess iron deposits in tissues. male wistar rats were fed a torula yeast-based se-deficient [se(-)] or se-adequate [se(+); containing 0.1 mg se/kg as sodium selenite] diet for 8 or 82 weeks. excised tissues were embedded in either paraffin or epoxy resin. a ...19958534984
the acidic ribosomal proteins of different yeast species. phosphorylation by ribosome-associated protein kinases.two major ribosomal proteins of mr 13 kda and 38 kda were identified as phosphorylation substrates for ribosome-bound protein kinases from different yeast species. the phosphorylation level was much higher in ribosomes from the cells collected at the exponential growth phase, than in those from the stationary phase. isoelectrofocusing of the protein band of 13 kda and subsequent silver staining allowed to identify from four in the case of pichia stipitis up to ten in saccharomyces cerevisiae. sa ...19958537876
isolation and characterization of mitochondrial nucleoids from the yeast pichia jadinii.mitochondrial (mt) nucleoids were isolated with a high degree of purity from the yeast pichia jadinii, in which the mitochondrial dna (mtdna) is linear. field-inversion gel electrophoresis (fige) revealed that significant amounts of mtdna could be isolated intact, as linear molecules of 41 kbp, from the isolated mt-nucleoids. fifteen different proteins were detected in the mt-nucleoid fraction and, eight of these proteins bound to dna. the patterns of mt-nucleoid proteins and of the dna-binding ...19968888620
mechanical responses of chromium-deficient developing rat heart.mechanical responses were compared between controls, developing sprague-dawley rat papillary muscle and age-matched weanlings fed with torula yeast, a food source deficient in chromium. at 8 weeks postnatal, deficient rats differed in significant ways from their normal counterparts. deficient rats in contrast to controls weighed less, their interval-force (i-f) relationship was more negative and their inotropic response to high calcium concentrations was greater. at this time, however, deficient ...19968925433
antimicrobial action of essential oils: the effect of dimethylsulphoxide on the activity of cinnamon oil.fifty-one essential oils extracted from plants of known origin were tested for their antimicrobial activity against three bacteria, pseudomonas aeruginosa, staphylococcus aureus, escherichia coli and four yeasts, torulopsis utilis, schizosaccharomyces pombe, candida albicans and saccharomyces cerevisiae using the drop diffusion method. all showed antimicrobial activity against at least one of the micro-organisms. following this preliminary screening, 13 essential oils showing antimicrobial activ ...19979134774
structure and function of 5s ribosomal ribonucleic acid from torulopsis utilis. i. purification and complete digestion with pancreatic ribonuclease a and ribonuclease t. 19744476732
influence of dietary selenium on the disposition of arsenate in the female b6c3f1 mouse.interactions between arsenic (as) and selenium (se) at the metabolic level are multifaceted and complex. these interactions are of practical significance because populations in various parts of the world are simultaneously exposed to inorganic as in drinking water and se mainly in the diet at varying levels. the primary goal of this study was to investigate whether differing dietary se status would alter the profile of urinary metabolites or their time course for elimination after exposure to ar ...19979183383
cellular glutathione peroxidase knockout mice express normal levels of selenium-dependent plasma and phospholipid hydroperoxide glutathione peroxidases in various tissues.selenium-dependent cellular glutathione peroxidase (gpx1) knockout [gpx1(-)] mice were derived from 129/svj x c57bl/6 hybrid mice by microinjecting c57bl/6 blastocysts with recombinant embryonic stem cells carrying a target mutation in the gpx1 gene. experiment 1 was conducted to determine the effects of the gpx1 knockout on the susceptibility of mice to dietary vitamin e and se deficiency and on the expression of the se-dependent plasma glutathione peroxidase (gpx3) and phospholipid hydroperoxi ...19979237936
rapid physiological characterization of microorganisms by biosensor technique.eleven microorganisms, arxula adeninivorans ls3, candida boidinii dsm 70034, candida lactis-condensi dsm 70635, pichia jadinii dsm 2361, pichia minuta dsm 7018, kluyveromyces lactis dsm 4394, pseudomonas putida dsm 50026, alcaligenes sp. dsm 30002, arthrobacter nicotianae dsm 20123 as well as issatchenkia orientalis dsm 70077 and rhodococcus erythropolis dsm 311 were characterized by the sensor technique by injection of 30 different substrates and substrate mixtures. the obtained data which are ...19979352658
bioavailability of selenium from veal, chicken, beef, pork, lamb, flounder, tuna, selenomethionine, and sodium selenite assessed in selenium-deficient rats.the bioavailability of selenium (se) from veal, chicken, beef, pork, lamb, flounder, tuna, selenomethionine (semet), and sodium selenite was assessed in se-deficient fischer-344 rats. se as veal, chicken, beef, pork, lamb, flounder, tuna, semet, and sodium selenite was added to torula yeast (ty) basal diets to comprise se-inadequate (0.05 mg se/kg) diets. se as sodium selenite was added to a ty basal diet to comprise a se-adequate (0.10 mg se/kg), se-control diet. the experimental diets were fed ...19979363319
effects of dietary selenium and vitamin e on boar performance and tissue responses, semen quality, and subsequent fertilization rates in mature gilts.three experiments involving 192 crossbred boars evaluated the effects of dietary se (0 or .5 ppm) and vitamin e (0 or 220 iu/kg) on growth, tissue se, and alpha-tocopherol concentrations, and on semen quality and its subsequent effect on fertilization rate in mature gilts. diets formulated used torula yeast and dextrose or cornstarch as the basal feedstuffs and were provided from weaning through sexual maturity. the basal diets averaged .063 ppm se and 3.46 mg alpha-tocopherol/kg diet. experimen ...19979374315
effects of dietary selenium and vitamin e concentrations on phospholipid hydroperoxide glutathione peroxidase expression in reproductive tissues of pubertal maturing male rats.phospholipid hydroperoxide glutathione peroxidase (phgpx) is the second intracellular selenium (se)-dependent glutathione peroxidase (gsh-px) identified in mammals. our objectives were to determine the effect of dietary vitamin e and se levels on phgpx activity expression in testis, epididymis, and seminal vesicles of pubertal maturing rats, and the relationship of phgpx expression with testicular development and sperm quality. forty sprague-dawley male weanling rats (21-d old), were initially f ...19979522062
selenium regulates gene expression for estrogen sulfotransferase and alpha 2u-globulin in rat liver.dietary intake of the essential trace element selenium (se) regulates expression of genes for selenoproteins and certain non-se-containing proteins. however, these proteins do not account for all of se's biological effects. the objective of this work was to identify additional genes whose expression is regulated by se. identification of these genes may reveal new functions for se or define mechanisms for its biological effects. weanling male sprague-dawley rats were fed a torula yeast-based se-d ...19989618024
knockout of cellular glutathione peroxidase affects selenium-dependent parameters similarly in mice fed adequate and excessive dietary selenium.this study was to determine whether or not effects of the cellular glutathione peroxidase (gpx1) knockout on several se-dependent parameters in mice were tissue, dietary se concentration, and selenoprotein specific. a 2 x 3 factorial experiment was conducted with 18 gpx1 knockout mice [gpx1(-)] and 18 controls (3 weeks old, half males and females). these mice were fed a torula yeast diet supplemented with all-rac-alpha-tocopheryl acetate (50 mg/kg of feed) and se (sodium selenite) at 0, 0.5, or ...19989666319
pteroyl- and tetrahydropteroylpolyglutamate effects on the catalytic activity of thymidylate synthase from lactobacillus leichmannii: a novel method for determining gamma-glutamyl chain lengths of the folylpolyglutamates.tetrahydropteroylpolyglutamates with longer gamma-glutamyl chain lengths have been found to act as better cofactors than the corresponding monoglutamates for the activity of thymidylate synthase, (5, 10-ch2h4pteglu: dump c-methyltransferase, ec 2.1.1.45) purified from lactobacillus leichmannii. contrarily, the pteroylpolyglutamates (unreduced forms) with longer gamma-glutamyl chain lengths act as powerful inhibitors of the same enzyme, the i50 being 2 microm for the tetraglutamate, and inhibitio ...19947959844
bioavailability of selenium from raw and cooked ground beef assessed in selenium-deficient fischer rats.the literature on the bioavailability of selenium (se) from meats, especially beef, is meager, and that which existed when this research began suggested that se was not highly bioavailable. in addition, much of the analytical values for se in beef predated the food and drug administration's 1973 approval of se as an additive to feeds and mineral premixes of livestock.19948157862
pichia lachancei sp. nov., associated with several hawaiian plant species.a description is given of pichia lachancei sp. nov., a new species of yeast that occurs in association with several hawaiian plant species of the genera tetraplasandra, cheirodendron and clermontia. the new species is heterothallic and occurs in nature in the haploid as well as the diploid state. upon conjugation of complementary mating types, zygotes are formed that reproduce by budding as diploid cells. when placed on sporulation medium, four hat-shaped spores are produced which are rapidly re ...199910425793
high levels of dietary vitamin e do not replace cellular glutathione peroxidase in protecting mice from acute oxidative stress.our objective was to determine whether high levels of dietary vitamin e replaced the protection of the se-dependent cellular glutathione peroxidase (gpx1) against paraquat- or diquat-induced acute oxidative stress in mice. two experiments were conducted using gpx1 knockout [gpx1(-/-)] mice and wild-type (wt) mice (n = 78/group). in experiment 1, mice were fed torula yeast-based, se-adequate (0.4 mg/kg as sodium selenite) diets + 0, 75, 750 or 7,500 mg all-rac-alpha-tocopheryl acetate for 5 wk be ...199910539768
[urinary excretion of s 35 after application of torula utilis marked with s 35; investigation of the time element of the intestinal protein metabolism in man by use of radioactive sulfur]. 195212977753
the synthesis of glutathione in torula utilis studied with 14c-carboxyl labeled glycine. 195212977787
selenium from high selenium broccoli protects rats from colon cancer.colon cancer is the third most common newly diagnosed cancer in the united states and the third most common cause of cancer-related deaths. previous supplementation studies have demonstrated the efficacy of selenium (se) for prevention of colon cancer in humans. the metabolism of se depends on its chemical form, and studies have shown that the chemical form of se in broccoli does not accumulate in the body as fast as other forms of se and may be especially beneficial for prevention of cancer. in ...200010958840
selenium regulates expression in rat liver of genes for proteins involved in iron metabolism.suppression subtractive hybridization analysis in our laboratory recently revealed that transferrin mrna may be elevated in sedeficient rat liver. in this work, we compared expression in rat liver of genes for transferrin, transferrin receptor, ferritin light and heavy chains, and iron-regulatory proteins 1 and 2 in se adequacy and deficiency. weanling male sprague-dawley rats were fed torula yeast diets supplemented with 0 or 0.15 microg se/kg diet as sodium selenite for 15 wk. activity of cell ...200011049200
dietary selenium and arsenic affect dna methylation in vitro in caco-2 cells and in vivo in rat liver and colon.selenium is an essential trace element for human health, and it has received considerable attention for its possible role as an anticarcinogenic agent. the purpose of the present study was to determine whether changes in the amount and the chemical form of selenium would affect dna methylation and whether this effect would be modified by arsenic. caco-2 cells, a human colon cancer cell line, were exposed to 0, 1 or 2 micromol supplemental selenite/l and 0, 1 or 2 micromol supplemental arsenite/l ...200011110844
promotion of lipid oxidation by selenate and selenite and indicators of lipid peroxidation in the rat.the ain-93 reformulation of the ain-76a rodent diet includes a change in selenium supplement from sodium selenite to sodium selenate to reduce dietary lipid peroxidation. a change to selenate as the standard form of se in rat diets would render results from previous work using selenite less relevant for comparison with studies using the ain-93 formulation. to critically examine the rationale for the ain-93 recommendation, we prepared torula yeast basal diets patterned as closely as possible afte ...200111354350
selenium from selenium-rich spirulina is less bioavailable than selenium from sodium selenite and selenomethionine in selenium-deficient rats.the bioavailabilty of selenium (se) from selenium-rich spirulina (sesp) was assessed in se-deficient rats by measuring tissue se accumulation and glutathione peroxidase (gsh-px) activity. for 42 d, rats were subjected to dietary se depletion by consumption of a torula yeast (ty)-based diet with no se; controls were fed the same diet supplemented with 75 microg se/kg diet as sodium selenite. se-deficient rats were then repleted with se (75 microg/kg) by the addition of sodium selenite, selenometh ...200111533277
experimental schistosomiasis mansoni in mice maintained on nutritionally deficient diets. i. effects of a torula yeast ration deficient in factor 3, vitamin e, and cystine. 195713429435
experimental schistosomiasis mansoni in mice maintained on nutritionally deficient diets. ii. survival and development of schistosoma mansoni in mice maintained on a torula yeast diet deficient in factor 3, vitamin e, and cystine. 195713429436
gene expression profiling of low selenium status in the mouse intestine: transcriptional activation of genes linked to dna damage, cell cycle control and oxidative stress.the essential trace mineral selenium (se) has been shown previously to inhibit intestinal, prostate, lung and liver tumor development and associated mortality in both experimental animals and humans. although se is likely to be one of the most powerful cancer chemopreventive agents in the human diet, its mechanism of action is unknown. to better understand the biological consequences of alterations in se status, the gene expression profile associated with low se status in the intestine of c57bl/ ...200111739862
the exudative diathesis produced by torula yeast. 195713512289
the acceleration of vitamin e deficiency in the chick by torula yeast. 195813514533
in vitro function of s rnases in lycopersicon peruvianum.s rnases are products of the s locus that are expressed in the stylar tissue of lycopersicon peruvianum with the gametophytic self-incompatibility (si) system. two s rnases (s12 and sa) with rnase activity from the s12sa genotype of l. peruvianum were purified using gel filtration and cation-exchange chromatography. the molecular masses of the two rnases, s12 and sa, were 21 and 23.1 kda, respectively. the specific activities of s12 and sa for torula yeast rrna as a substrate were 8,500 and 6,00 ...200111804331
dietary selenite and azadeoxycytidine treatments affect dimethylhydrazine-induced aberrant crypt formation in rat colon and dna methylation in ht-29 cells.several observations implicate a role for altered dna methylation in cancer pathogenesis. the global level of dna methylation is generally lower; however, dna methyltransferase (dnmt1) activity is usually higher in tumor cells than in normal cells. the purpose of this study was to investigate whether the dnmt1 inhibitor, 5-aza-2'-deoxycytidine (aza-dc) would alter the effect of dietary selenium on the formation of aberrant crypts. weanling rats (n = 60) were fed three concentrations of selenium ...200211823593
antinecrogenic property of torula yeast treated in various ways. 195913642134
[study of the lag phase of microorganisms. i. influence of external conditions on the length of the lag phase in torulopsis utilis and pseudomonas fluorescens]. 195913674051
selenium and torula yeast in production of exudative diathesis in chicks. 196013739146
parameters of dietary selenium and vitamin e deficiency in growing rabbits.4 x 5 growing female rabbits (new zealand white) with an initial live weight of 610 +/- 62 g were fed a torula yeast based semisynthetic diet low in selenium (<0.03 mg/kg diet) and containing <2 mg alpha-tocopherol per kg (group i). group ii received a vitamin e supplementation of 150 mg alpha-tocopherylacetate per kg diet, whereas for group iii 0.40 mg se as na-selenite and for group iv both supplements were added. selenium status and parameters of tissue damage were analyzed after 10 weeks on ...200211878752
nitrogen balance of young adults consuming a deficient diet supplemented with torula yeast and other nitrogenous products. 195913828648
the isolation and kinetics of two forms of fumarase from torula yeast. 196113905668
overexpression of cellular glutathione peroxidase does not affect expression of plasma glutathione peroxidase or phospholipid hydroperoxide glutathione peroxidase in mice offered diets adequate or deficient in selenium.selenium-dependent cellular glutathione peroxidase (gpx1) overexpressing [gpx1(+)] mice were derived by microinjecting a 5.3-kb cloned entire mouse gpx1 genomic dna into fertilized eggs. the objective of this study was to determine the effect of gpx1 overexpression and dietary selenium on the expression of selenoperoxidases and the status of lipid peroxidation of these transgenic animals. an experiment with a 2 x 2 factorial arrangement of treatments with 15 gpx1(+) and 15 control mice (2 mo old ...19979164985
effects of brewer's yeast on glucose tolerance and serum lipids in chinese adults.this study was designed to investigate the supplemental effects of brewer's yeast on serum glucose and lipids in chinese adults. twenty-two participants (8 males and 14 females) were recruited from pingtung city. mean age of the group was 51 yr, and fasting values of total cholesterol and glucose were from 3.21 to 6.90 and 4.3 to 6.2 mmol/l, respectively. before supplementation, a 75 g oral glucose load was administered after an overnight (9 h) fast. blood was drawn before and at 30, 60, 90, and ...19947946924
laboratory model with chicks for assay of nutritional factors affecting hepatic lipid accumulation in laying hens.a laboratory model with chicks is described that is suggested as an assay for dietary factors affecting liver lipid accumulation in laying hens. the model utilizes growing white leghorn male chicks injected with estrogen. diets to be tested were fed ad libitum for 4 days following 2 days starvation. liver lipid deposition was increased with increasing level of estradiol injected. lipid deposition in livers of estrogenized chicks fed a diet containing fish meal, alfalfa meal, and torula yeast (fa ...19836828406
antimicrobial substances from aspen tissue grown in vitro.isolated aspen tissue, when grown in vitro for 3 weeks on agar medium, yielded antimicrobial substances which produced inhibitory zones when the culture plates were inoculated with fusarium roseum, saccharomyces cervisiae, bacillus subtilis, bacillus cereus, penicillium, roqueforti, torula utilis, sarcina lutea, flavobacterium aquatile, pullularia pullulans, and staphylococcus aureus.196313933593
bioavailability of selenium from raw or cured selenomethionine-enriched fillets of atlantic salmon (salmo salar) assessed in selenium-deficient rats.the bioavailability of se from raw and cured selenomethionine-enriched (se-enriched) salmon fillets was assessed in se-deficient male albino rats (mol: wist). a low-se torula yeast feed was supplemented with 0, 50, 100, 150 or 200 microg se/kg as sodium selenite or as se from raw or cured se-enriched salmon. the diets were fed to weanling rats for 10 and 30 d. bioavailability of se was assessed by metabolic balance, se accumulation in femur, muscle, liver and plasma, and induction of se-dependen ...200211895311
[on the identity of candida utilis and pichia jadinii yeast species].life cycles of candida utilis (henneberg) lodder et kreger-van rij imperfect yeast (13 strains) used in industry were the study subject. when the strains were mated, we detected one of the stages of sexual process--conjugation of cells of the opposite mating type. most of the studied cultures conjugated on the 2d-3d day. no ascospores were formed. haploidy and heterothallism of the studied c. utilis strains were confirmed by hybridization of auxotrophic mutants. based on pcr assay results, the y ...200211944341
structure of wyosine, the condensed tricyclic nucleoside of torula yeast phenylalanine transfer ribonucleic acid.large-scale isolation of the minor nucleoside wyosine of torula yeast trna(phe) was accomplished by a combination of enzymatic digestion and reversed-phase chromatography: the wyosine-containing nucleotide fraction, which was obtained by partial digestion of unfractionated trna (1 g) with nuclease p1, was concentrated by reversed-phase column chromatography followed by complete digestion with nuclease p1/alkaline phosphatase. the nucleoside mixture thus obtained was purified by reversed-phase hp ...200211964008
all-vegetable protein mixtures for human feeding. x. effect of torula yeast on the protein quality of incap vegetable mixture 9. 196213992597
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