Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
evaluation of food lures for capture and monitoring of anastrepha fraterculus (diptera: tephritidae) on temperate fruit trees.the anastrepha fraterculus (wiedemann) (diptera: tephritidae) is the main pest of fruit trees grown in temperate climates in the southern region of brazil. the objective of this work was to evaluate the efficiency of the major commercial food lures used in brazil for trapping and monitoring of a. fraterculus in plum, pear, and feijoa orchards. the assessed lures were hydrolyzed proteins of animal origin (ceratrap) and plant origin (bioanastrepha), torula yeast + borax (torula), and grape juice. ...201728334322
two-dimensional fluorescence difference spectroscopy to characterize nanoparticles and their interactions.two dimensional fluorescence difference spectroscopy (2d fds) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths. comparing metal oxide nanoparticles revealed a unique spectral signature per material composition. 2d fds showed to be sensitive to changes in surface properties between zno nps synthesized by different methods. zno np loaded with glycol c ...201627624316
genetic determinants for enhanced glycerol growth of saccharomyces cerevisiae.the yeast saccharomyces cerevisiae generally shows a low natural capability to utilize glycerol as the sole source of carbon, particularly when synthetic medium is used and complex supplements are omitted. nevertheless, wild type isolates have been identified that show a moderate growth under these conditions. in the current study we made use of intraspecies diversity to identify targets suitable for reverse metabolic engineering of the non-growing laboratory strain cen.pk113-1a. a genome-wide g ...201626971668
enhanced treatment of tannery wastewater in an integrated multistage bioreactor (imbr) by the predominant bacterial strains enriched from marine sediments.an innovative integrated multistage bioreactor (imbr) system, which was augmented with three predominant bacterial strains (lactobacillus paracasei cl1107, pichia jadinii cl1705, and serratia marcescens cl1502) isolated from marine sediments, was developed to treat real tannery wastewater without performing physicochemical pretreatment, with the potential to reduce the generation of waste sludge and odors. the performance of the imbr treatment system, with and without the inclusion of the predom ...201626901723
comprehensive analysis of the skin fungal microbiota of astronauts during a half-year stay at the international space station.the international space station (iss) is a huge manned construct located approximately 400 km above the earth and is inhabited by astronauts performing space experiments. because the station is within a closed microgravity environment, the astronauts are subject to consistent stress. this study analyzed the temporal changes in the skin fungal microbiota of 10 astronauts using pyrosequencing and quantitative pcr assay before, during, and after their stay in the iss. lipophilic skin fungi, malasse ...201626773135
[trioses and pyruvic acid, intermediate products in the aerobic breakdown of pentoses with torula utilis]. 201615435539
the comparative analysis of antioxidant and biological activity for the dendropanax morbifera lev. leaves extracted by different ethanol concentrations.the different concentrations of ethanol (20-100%) and distilled water extract for dendropanax morbifera lev. leaves were evaluated to induce antioxidant and biological activity employed by variety of assays. the 20%, 80%, and 100% ethanol extract demonstrated the relatively higher activity, whereas distilled water, 40%, and 60% ethanol extracts exhibited the lower antioxidant and biological activity. especially, 80% ethanol extract showed the remarkably higher radical scavenging activity, reduci ...201627592831
candida utilis and cyberlindnera (pichia) jadinii: yeast relatives with expanding applications.the yeast candida utilis is used as a food additive and as a host for heterologous gene expression to produce various metabolites and proteins. reliable protocols for intracellular production of recombinant proteins are available for c. utilis and have now been expanded to secrete proteins into the growth medium or to achieve surface display by linkage to a cell wall protein. a recombinant c. utilis strain was recently shown to induce oral tolerance in a mouse model of multiple sclerosis suggest ...201627357226
grape juice as a bait for anastrepha suspensa (diptera: tephritidae) and zaprionus indianus (diptera: drosophilidae).in field tests conducted in south florida to test grape juice as a bait for the caribbean fruit fly, anastrepha suspensa loew, high numbers of zaprionus indianus gupta were captured in traps with aqueous grape juice. these experiments included comparisons of grape juice bait with established a. suspensa protein-based baits (ammonium acetate + putrescine lures, or torula yeast) or wine, a bait found previously to be attractive to z. indianus. effects of different preservatives (polypropylene glyc ...201526470354
the structure of the cyberlindnera jadinii genome and its relation to candida utilis analyzed by the occurrence of single nucleotide polymorphisms.the yeast cyberlindnera jadinii is a close relative of candida utilis that is being used in the food and feed industries. here, we present the 12.7mb genome sequence of c. jadinii strain cbs 1600 generated by next generation sequencing. the deduced draft genome sequence consists of seven large scaffolds analogous to the seven largest chromosomes of c. utilis. an automated annotation of the c. jadinii genome identified 6147 protein-coding sequences. the level of ploidy for both genomes was analyz ...201526150016
purification and characterization of an extracellular uricase from a new isolate of sphingobacterium thalpophilum (vitpcb5).an extracellular uricase producing bacterium (vitpcb5) was isolated from soil of the duck farm near chidambaram, tamilnadu, india and it was identified based on its 16s rrna as sphingobacterium thalpophilum. uric acid was used as an effective inducer. the enzyme kinetics was studied using uric acid as a substrate. the km and vmax for the enzyme was found to be 0.28mm and 0.92μm/minml, respectively. maximum uricase production was observed when lactose was used as a carbon source. among the nitrog ...201526145833
rna-mediated cds-based nanostructures.the colloidal method provides an important tool to synthesize different nanostructures and their assemblies. the coating of colloidal inorganic nanostructures by the biotemplate of similar dimension not only provides them the thermodynamic stability but also imparts the chemical features to grow in different dimensionalities and capabilities for molecular recognition. in this chapter we describe detailed methodology for the synthesis and characterization of cds nanoparticles templated by ribonuc ...201525967063
asymmetric reduction of 4-hydroxy-2-butanone to (r)-1,3-butanediol with absolute stereochemical selectivity by a newly isolated strain of pichia jadinii.in this study, a novel strain of pichia jadinii, hby61, capable of the biocatalysis of 4-hydroxy-2-butanone (4h2b) to (r)-1,3-bd was isolated. hby61 produced (r)-1,3-bd with high activity and absolute stereochemical selectivity (100 % e.e). glucose and beef extract were found to be the key factors governing the fermentation, and their optimal concentrations were determined to be 84.2 and 43.7 g/l, respectively. the optimal bioconversion conditions of 4h2b catalyzed by hby61 were ph 7.4, 30 °c, a ...201425306883
regioselective hydrolysis of acetates in the presence of different yeast strains.the model compound, hexane-1,2-diol diacetate, was hydrolyzed in the presence of supernatant obtained after cultivation of 4 yeast strains: pichia jadinii, rhodotorula glutinis and yarrowia lipolytica kkp 379 and saccharomyces cerevisiae 102 to evaluate the type of catalysis. the regioselectivity of extracellular enzymes as a function of hydrolysis towards primary and secondary acetic acid ester groups was monitored. the enzymes secreted by p. jadinii, r. glutinis and y. lipolytica kkp 379 exhib ...201425272729
comparison of food-based attractants for bactrocera invadens (diptera: tephritidae) and evaluation of mazoferm-spinosad bait spray for field suppression in mango.catches of bactrocera invadens drew, tsuruta, & white (diptera: tephritidae) in multi-lure traps baited with six commercial food-based attractants, mazoferm e802, torula yeast, gf-120, hymlure, biolure, and nulure were compared in mango orchards in kenya. in 2007, mazoferm e802 and torula yeast were the most effective attractants and captured 2.4-2.6 times more females and 3.4-4.0 times more males than the standard nulure. all attractants captured more females than males (ranging from 63 to 74%) ...201424665714
carbon source utilization and inhibitor tolerance of 45 oleaginous yeast species.conversion of lignocellulosic hydrolysates to lipids using oleaginous (high lipid) yeasts requires alignment of the hydrolysate composition with the characteristics of the yeast strain, including ability to utilize certain nutrients, ability to grow independently of costly nutrients such as vitamins, and ability to tolerate inhibitors. some combination of these characteristics may be present in wild strains. in this study, 48 oleaginous yeast strains belonging to 45 species were tested for abili ...201424818698
comparison of torula yeast and various grape juice products as attractants for mexican fruit fly (diptera: tephritidae).early research investigating attractants for the mexican fruit fly, anastrepha ludens loew, during the 1930s indicated that fermentation products were effective attractants for mexican fruit flies and other tropical tephritidae, but that attraction to fruit components was only of academic interest. tests reported here were carried out on populations of mexican fruit flies from 2004 to 2011. trapping experiments carried out at sites in the states nuevo leon and san luis potosi compared grape juic ...201424772538
[study of the cytochromic system of torula utilis by the distribution of the reduction rates of the chain links during steady state]. 20145887015
[massive pathogenicity produced by a non-virulent mycete (torulopsis utilis)]. 201413315402
[stabilization of the properties of a test culture of torula utilis for determining the antifungal activity of antibiotics]. 20144175970
biocontrol activity of four non- and low-fermenting yeast strains against aspergillus carbonarius and their ability to remove ochratoxin a from grape juice.aspergillus spp. infection of grape may lead to ochratoxin a (ota) contamination in processed beverages such as wine and grape juice. the aim of the current study was to evaluate the biocontrol potential of two non-fermenting (cyberlindnera jadinii 273 and candida friedrichii 778) and two low-fermenting (candida intermedia 235 and lachancea thermotolerans 751) yeast strains against the pathogenic fungus and ota-producer aspergillus carbonarius, and their ability to remove ota from grape juice. t ...201425113045
analysis of microflora profile in korean traditional nuruk.a variety of nuruk were collected from various provinces in korea, and their microflora profiles were analyzed at the species level. a total of 42 nuruk samples were collected and when the viable cell numbers in these nuruk were enumerated, the average cell numbers of bacteria, fungi, yeast, and lactic acid bacteria from all nuruk were 7.21, 7.91, 3.49, and 4.88 log cfu/10 g, respectively. there were no significant differences in viable cell numbers of bacteria or fungi according to regions coll ...201323314366
heterologous protein secretion by candida utilis.the yeast candida utilis (also referred to as torula) is used as a whole-cell food additive and as a recombinant host for production of intracellular molecules. here, we report recombinant c. utilis strains secreting significant amounts of candida antarctica lipase b (calb). native and heterologous secretion signals led to secretion of calb into the growth medium; calb was enzymatically active and it carried a short n-glycosyl chain lacking extensive mannosylation. furthermore, calb fusions to t ...201323613034
selenium status alters the immune response and expulsion of adult heligmosomoides bakeri worms in mice.heligmosomoides bakeri is a nematode with parasitic development exclusively in the small intestine of infected mice that induces a potent stat6-dependent th2 immune response. we previously demonstrated that host protective expulsion of adult h. bakeri worms from a challenge infection was delayed in selenium (se)-deficient mice. in order to explore mechanisms associated with the delayed expulsion, 3-week-old female balb/c mice were placed on a torula yeast-based diet with or without 0.2 ppm se, a ...201323649095
modelling of biological cr(vi) removal in draw-fill reactors using microorganisms in suspended and attached growth systems.the kinetics of hexavalent chromium bio-reduction in draw-fill suspended and attached growth reactors was examined using sugar as substrate and indigenous microorganisms from the industrial sludge of the hellenic aerospace industry. initially, experiments in suspended growth batch reactors for cr (vi) concentrations of 1.4-110 mg/l were carried out, to extensively study the behaviour of a mixed culture. the maximum cr(vi) reduction rate of 2 mg/l h was achieved for initial concentration 12.85 mg ...201323182668
performance of the wheat armyworm, pseudaletia sequax franclemont, on natural and artificial diets.armyworms in the genus pseudaletia franclemont are important pests of cereal crops worldwide. the development of pest management strategies is largely dependent on studies involving the relationship among the pest, its host plants and natural enemies, as well as its susceptibility to chemical and biological control strategies. part of these studies requires rearing the species of interest under laboratory conditions, which can be greatly facilitated by the use of artificial diets. in the present ...201223950064
knockout of sod1 alters murine hepatic glycolysis, gluconeogenesis, and lipogenesis.we previously observed a stronger effect of knockout of cu,zn-superoxide dismutase (sod1) than that of se-dependent glutathione peroxidase 1 (gpx1) on murine body weight and glucose homeostasis. two experiments were conducted to determine how hepatic lipid profiles and key metabolic regulators were correlated with this difference. sod1(-/-) and gpx1(-/-) mice and their respective wild-type (wt) littermates (n=6 or 7/group, male) were fed a se-adequate torula yeast-sucrose diet and killed at 6 mo ...201222974764
field evaluation of attractive lures for the fruit fly bactrocera minax (diptera: tephritidae) and their potential use in spot sprays in hubei province (china).the chinese citrus fruit fly, bactrocera minax (enderlein) is a univoltine tephritidae pest that infests citrus species. field trials were conducted in 2010 to determine the potential use of a lure based on enzymatical-hydrolyzed beer yeast as liquid bait (hereafter named h-protein bait) for b. minax in the hubei province, china. in a citrus orchard, we compared the attractiveness among aqueous solutions of h-protein bait, gf-120 fruit fly bait, sugar-vinegar-wine mixture, torula yeast, and jufe ...201222928307
effect of dietary nucleotide supplementation on performance and development of the gastrointestinal tract of broilers.1. two experiments were conducted to determine the effects of dietary nucleotide supplementation on broiler performance, and physical and morphological development of the gastrointestinal tract. 2. experiment 1: a total of 180 one-d-old male chicks were placed in battery brooders in 3 × 6 replicate pens containing 10 chicks each. chicks were randomly assigned to one of the three dietary treatments; a maize-soyabean meal based diet supplemented with 0, 0·25, and 0·50% torula yeast rna (as a sourc ...201222404810
selenium bioavailability from naturally produced high-selenium peas and oats in selenium-deficient rats.this study determined the bioavailability of selenium (se) from yellow peas and oats harvested from the high-se soil of south dakota, united states. the se concentrations were 13.5 ± 0.2 and 2.5 ± 0.1 mg/kg (dry weight) for peas and oats, respectively. male weanling sprague-dawley rats were depleted of se by feeding them a 30% torula yeast-based diet (4.1 μg se/kg) for 56 days, and then they were replenished with se for an additional 50 days by feeding them the same diet supplemented with 20, 30 ...201121553810
both maximal expression of selenoproteins and selenoprotein deficiency can promote development of type 2 diabetes-like phenotype in mice.selenium (se) is an essential trace element in mammals that has been shown to exert its function through selenoproteins. whereas optimal levels of se in the diet have important health benefits, a recent clinical trial has suggested that supplemental intake of se above the adequate level potentially may raise the risk of type 2 diabetes mellitus. however, the molecular mechanisms for the effect of dietary se on the development of this disease are not understood. in the present study, we examined ...201121194350
nutritional and supranutritional levels of selenate differentially suppress prostate tumor growth in adult but not young nude mice.the inhibitory effect of oral methylseleninic acid or methylselenocysteine administration on cancer cell xenograft development in nude mice is well characterized; however, less is known about the efficacy of selenate and age on selenium chemoprevention. in this study, we tested whether selenate and duration on diets would regulate prostate cancer xenograft in nude mice. thirty-nine homozygous nu/j nude mice were fed a selenium-deficient, torula yeast basal diet alone (se-) or supplemented with 0 ...201122137259
Effect of Dietary Selenium and Cancer Cell Xenograft on Peripheral T and B Lymphocytes in Adult Nude Mice.Selenium (Se) is known to regulate tumorigenesis and immunity at the nutritional and supranutritional levels. Because the immune system provides critical defenses against cancer and the athymic, immune-deficient NU/J nude mice are known to gradually develop CD8(+) and CD4(+) T cells, we investigated whether B and T cell maturation could be modulated by dietary Se and by tumorigenesis in nude mice. Fifteen homozygous nude mice were fed a Se-deficient, Torula yeast basal diet alone (Se-) or supple ...201122020772
captures of pest fruit flies (diptera: tephritidae) and nontarget insects in biolure and torula yeast traps in hawaii.multilure traps were deployed in a hawaiian orchard to compare the attraction of economically important fruit flies and nontarget insects to the three-component biolure and torula yeast food lures. either water or a 20% propylene glycol solution was used to dissolve the torula yeast or as capture fluid in biolure traps. torula yeast in water was more attractive than biolure for male and female bactrocera cucurbitae (coquillett) and bactrocera dorsalis (hendel) and as attractive for ceratitis cap ...201022546461
effective sampling range of food-based attractants for female anastrepha suspensa (diptera: tephritidae).release-recapture studies were conducted with both feral and sterile females of the caribbean fruit fly, anastrepha suspensa (loew) (diptera: tephritidae), to determine sampling range for a liquid protein bait (torula yeast/borax) and for a two-component synthetic lure (ammonium acetate and putrescine). tests were done in a guava, psidium guajava l., grove and involved releasing flies at a central point and recording the numbers captured after 7 h and 1, 2, 3, and 6 d in an array of 25 multilure ...201020429471
the effect of carbon source on microbial community structure and cr(vi) reduction rate.in the present work, the effect of the carbon source on microbial community structure in batch cultures derived from industrial sludge and hexavalent chromium reduction was studied. experiments in aerobic batch reactors were carried out by amending industrial sludge with two different carbon sources: sodium acetate and sucrose. in each of the experiments performed, four different initial cr(vi) concentrations of: 6, 13, 30 and 115 mg/l were tested. the change of carbon source in the batch reacto ...201020552669
endogenous fraction and urinary recovery of purine derivatives obtained by different methods in nellore cattle.two experiments were conducted to assess the endogenous fraction of purine derivative (pd) excretion, urinary recovery, and intestinal digestibility of purines in nellore heifers. for both experiments, 8 nellore heifers fitted with ruminal and abomasal cannulas were allocated to two 4 × 4 latin squares. the diets were based on corn silage and concentrate (60 and 40% dm basis, respectively); feces and urine samples were obtained by total collection, and abomasal dm flow was estimated using indige ...201020889688
assessment of selenium bioavailability from naturally produced high-selenium soy foods in selenium-deficient rats.we assessed the bioavailability of selenium (se) from a protein isolate and tofu (bean curd) prepared from naturally produced high-se soybeans. the se concentrations of the soybeans, the protein isolate and tofu were 5.2±0.2, 11.4±0.1 and 7.4±0.1mg/kg, respectively. male weanling sprague-dawley rats were depleted of se by feeding them a 30% torula yeast-based diet (4.1μg se/kg) for 56 days, and then they were replenished with se for an additional 50 days by feeding them the same diet containing ...201021093782
diversity and seasonality of fruit flies (diptera: tephritidae and lonchaeidae) and their parasitoids (hymenoptera: braconidae and figitidae) in orchards of guava, loquat and peach.this work was carried out in orchards of guava progenies, and loquat and peach cultivars, in monte alegre do sul, sp, brazil, in 2002 and 2003. guavas and loquats were bagged and unbagged bi-weekly and weekly, respectively, for assessment of the infestation period. peach was only bagged weekly. the assays started when the fruits were at the beginning of development, but still green. ripe fruits were taken to the laboratory and placed individually into plastic cups. mcphail plastic traps containi ...200919347143
self assembly of poly(o-methoxy aniline) with rna and rna/dna hybrids: physical properties and conformational change of poly(o-methoxy aniline).biomolecular hybrids of a conducting polymer [poly(o-methoxy aniline) (poma)] and rna are prepared at the three different compositions by mixing aqueous solutions of diethyl, 2-hydroxy ethyl, ammonium salt of rna (type ix from torula yeast) and poma (es, emeraldine salt; doping level [cl]/[n]=0.52). a slow increase of ph up to 30 h of aging occurs in the mixture till it levels up. the tem micrographs indicate a fibrillar network structure in all the hybrid compositions (poma: rna=1:3, 1:1, 3:1, ...200919482408
evaluation of yeasts and yeast products in larval and adult diets for the oriental fruit fly, bactrocera dorsalis, and adult diets for the medfly, ceratitis capitata, and the melon fly, bactrocera curcurbitae.several yeasts and yeast products were tested in adult diets for the medfly ceratitis capitata (wiedemann), oriental fruit fly bactrocera dorsalis (hendel), and melon fly, bactrocera curcurbitae (coquillett) (diptera: tephritidae) and in larval liquid diet for mass-rearing b. dorsalis. three hydrolyzed brewer's yeasts (fnils65, fni200 and fni210), one glutamine enriched yeast (gsh), one vitamin-enriched yeast (rda500), korea yeast, whole cell yeasts, and combinations of them were evaluated. adul ...200919613830
irreversible thermoinactivation of ribonuclease-a by soft-hydrothermal processing.ribonuclease (rnase), which often represents molecular biological contamination, is a thermostable enzyme. when rnase is heated at 121 degrees c by autoclave sterilization for 20 min, it does not lose its activity. however, the nature of the molecular events by which the irreversible denaturation occurs remains unknown. the purpose of this study was to elucidate the molecular mechanisms of irreversible thermal denaturation of rnase a and to develop an advanced sterilization method using soft-hyd ...200919725109
selenium bioavailability from soy protein isolate and tofu in rats fed a torula yeast-based diet.selenium (se) is an essential nutrient, and soy is a major plant source of dietary protein to humans. the united states produces one-third of the world's soybeans, and the se-rich northern plains produce a large share of the nation's soybeans. the present study used a rat model to determine the bioavailability of se from a protein isolate and tofu (bean curd) prepared from a soybean cultivar we recently developed specifically for food grade markets. the soybean seeds contained 2.91 mg se/kg. mal ...200919919094
effect of dietary selenium on the promotion of hepatocarcinogenesis by 3,3', 4,4'-tetrachlorobiphenyl and 2,2', 4,4', 5,5'-hexachlorobiphenyl.polychlorinated biphenyls (pcbs) are persistent organic pollutants that have promoting activity in the liver. pcbs induce oxidative stress, which may influence carcinogenesis. epidemiological studies strongly suggest an inverse relationship between dietary selenium (se) and cancer. despite evidence linking se deficiency to hepatocellular carcinoma and liver necrosis, the underlying mechanisms for se cancer protection in the liver remain to be determined. we examined the effect of dietary se on t ...200818296742
ribonuclease, deoxyribonuclease, and antiviral activity of escherichia coli-expressed bougainvillea xbuttiana antiviral protein 1.a full-length cdna encoding ribosome-inactivating/antiviral protein from the leaves of bougainvillea xbuttiana was recently isolated. the coding region of cdna was cloned and expressed in escherichia coli, and the protein product was designated as bbap1 (bougainvillea xbuttiana antiviral protein 1). bbap1 showed ribonuclease activity against torula yeast rna. it also exhibited depurination activity against supercoiled pbluescript sk+ plasmid dna in a concentration dependent manner, and was found ...200818393761
estimation of the selenium requirement of growing guinea pigs (cavia porcellus).the aim of the study was to determine the selenium (se) requirement of guinea pigs as a species unable to synthesize ascorbic acid. forty-nine male guinea pigs (average weight 208 +/- 3.5 g) were divided into an initial status group and six experimental groups. the animals received a se deficient torula yeast based basal diet (<0.02 mg se and 26 mg alpha-tocopherol/kg) or a se addition of 0.05, 0.10, 0.15, 0.20 and 0.25 mg/kg diet as sodium selenate for 10 weeks. there was no significant differe ...200818662358
effects of selenium and low levels of lead on mammary tumor development and growth in mmtv-infected female mice.selenium (se) has been demonstrated in previous studies to inhibit mammary tumorigenesis in c3h mice infected with the murine mammary tumorvirus, mmtv. the antitumorigenic effects of se in this animal model of breast cancer were subsequently shown to be counteracted by se-antagonistic elements. lead (pb), for example, was found to abolish the anticarcinogenic effects of se at 5 ppm in the drinking water. the present study was undertaken to explore the effects of pb at just 0.5 ppm in the water, ...200818726561
knockouts of se-glutathione peroxidase-1 and cu,zn superoxide dismutase exert different impacts on femoral mechanical performance of growing mice.the objective of this study was to determine the impact of knockout of cu,zn-superoxide dismutase (sod1) and se-glutathione peroxidase-1 (gpx1) on murine bone biomechanical properties. femora samples were collected from wild-type (wt), sod1-knockout [sod1(-/-)] and gpx1-knockout [gpx1(-/-)] female mice (9-wk old, n = 7-8 per genotype) to assay for bone enzyme activities and mechanical properties in three point bending. prior to testing, all mice were fed a torula yeast diet supplemented with 0.4 ...200818727007
dietary and tissue selenium in relation to methylmercury toxicity.selenium (se) supplementation in the nutritionally relevant range counteracts methylmercury (mehg) toxicity. since se tends to be abundant in fish, mehg exposures alone may not provide an accurate index of risk from fish consumption. molar ratios of mehg:se in the diets and hg:se in tissues of exposed individuals may provide a more accurate index. this experiment compared mehg toxicity in relation to mehg exposure vs. hg:se molar ratios in diets and tissues. diets were prepared using low-se toru ...200818761370
determination of selenium bioavailability from wheat mill fractions in rats by using the slope-ratio assay and a modified torula yeast-based diet.selenium is an essential mineral micronutrient for animals, and significant evidence supports an association between supranutritional se intake and a reduction in the incidence of some forms of cancer. thus, supplemental se intake may provide an avenue for reducing cancer incidence. however, an important issue to consider is the form of se that should be provided in such a supplement, because the bioavailability and bioactivity of se can vary dramatically depending on the chemical form in which ...200717227087
purification of an abf2p-like protein from mitochondrial nucleoids of yeast pichia jadinii and its role in the packaging of mitochondrial dna.a 26-kda protein with highly basic pi was purified from the mitochondrial (mt-) nucleoids of the yeast pichia jadinii by a combination of acid extraction, hydroxyapatite chromatography and dna-cellulose chromatography. the 26-kda protein has the ability to introduce a supercoil into circular plasmid dna in the presence of topoisomerase i and to package mtdna into nucleoid-like aggregates. the mt-nucleoids isolated from p. jadinii cells were disassembled in the presence of 2 m nacl and reassemble ...200717295092
importance of molar ratios in selenium-dependent protection against methylmercury toxicity.the influence of dietary selenium (se) on mercury (hg) toxicity was studied in weanling male long evans rats. rats were fed ain-93g-based low-se torula yeast diets or diets augmented with sodium selenite to attain adequate- or rich-se levels (0.1, 1.0 or 15 micromol/kg, respectively) these diets were prepared with no added methylmercury (mehg) or with moderate- or high-mehg (0.2, 10 or 60 micromol/kg, respectively). health and weights were monitored weekly. by the end of the 9-week study, mehg t ...200717916948
effect of physical restraint on oxidative stress in mice fed a selenium and vitamin e deficient diet.physical restraint has been associated with increased oxidative damage to lipid, protein, and dna. the purpose of this experiment was to determine whether physical restraint would further exacerbate oxidative stress in mice fed a selenium (se) and vitamin e (ve) deficient diet. three-week- old mice were fed a torula yeast diet containing adequate or deficient se and ve. menhaden oil was added to the deficient diet to impose an additional oxidative stress. after 4 wk feeding, half the mice in eac ...200616632897
[moulds and yeasts in bottled water and soft drinks].some damaged cartons of soft drinks and carbonated water were analyzed to detect the microorganisms that caused the damage. the contaminants of sugar used in the production of one of the drinks were also studied. the methods of déak & beuchat and pitt & hocking were used for the identification of yeasts and moulds, respectively. the agents of the spoilage of soft drinks were debaryomyces hansenii, debaryomyces polymorphus, galactomyces geotrichum, metschnikowia pulcherrima, mucor circinelloides, ...200617037258
female-biased attraction of oriental fruit fly, bactrocera dorsalis (hendel), to a blend of host fruit volatiles from terminalia catappa l.coupled gas chromatography-electroantennogram detection (gc-ead) analysis of volatiles from tropical almond fruit, terminalia catappa l., revealed 22 compounds that were detected by antennae of oriental fruit fly females, bactrocera dorsalis (hendel). both solid-phase microextraction (spme) and porapak q were used for sampling odors in fruit headspace, with spme collections producing larger ead responses from a greater number of compounds. geranyl acetate and methyl eugenol elicited the largest ...200617082987
identification of the genes gpd1 and gpd2 of pichia jadinii.two genes coding for proteins with a high degree of sequence similarity to glycerol-3-phosphate dehydrogenases have been isolated from the yeast pichiajadinii. fragments of the genes were pcr-amplified with degenerated primers from genomic dna of p. jadinii. clones containing the full-length genes pjgpdi and pjgpd2 were isolated by screening genomic libraries. dna sequencing revealed open reading frames (orfs) of 1182 bp and 1185 bp for pjgpdlp and pjgpd2p, respectively. in a complementation stu ...200617381046
effect of osmotic stress on the derepression of invertase synthesis in nonconventional yeasts.the aim of this study was to analyse the effect of osmotic stress on the biosynthesis of invertase enzyme in nonconventional yeasts.200616411924
an antiviral protein having deoxyribonuclease and ribonuclease activity from leaves of the post-flowering stage of celosia cristata.an antiviral protein named ccp-27 was purified from the leaves of celosia cristata at the post-flowering stage by anion-exchange, cation-exchange, and gel-filtration chromatography. it exhibited resistance against sunnhemp rosette virus in its test host cyamopsis tetragonoloba. it also exhibited deoxyribonuclease activity against supercoiled pbluescript sk+ plasmid dna. it was found to nick supercoiled dna into nicked circular form at lower protein concentration followed by nicked to linear form ...200616487067
primary hair growth in dogs depends on dietary selenium concentrations.selenium (se) plays an important role in hair growth. the objective of this study was to investigate the effect of dietary selenium concentration on hair growth in dogs. thirty-six beagles were stratified into six groups based on age, gender and body condition score. the dogs were fed a torula yeast-based canned food for 3 weeks. then the dogs were fed varying amounts of selenium supplied as selenomethionine for an additional 24 weeks. analysed selenium concentrations in the experimental foods f ...200616519759
[adaptation of yeasts to pentoses. iv. adaptation to arabinose of torulopsis utilis]. 200613674053
[effect of dicarbonic amino acids on proteins synthesis in torulopsis utilis]. 200513493076
[macrophagocytosis and cortisone, determined by a new quantitative method (physiurgic inoculation with torulopsis utilis)]. 200513315390
effect of intermittent supplementation with selenate on selenium status of rats fed selenium-deficient diet.to examine the selenium (se) status of rats intermittently supplemented with se, we measured tissue se contents and glutathione peroxidase (gpx) activities in rats fed a se-deficient diet intermittently supplemented with selenate. in experiment 1, four groups of male 4-wk-old wistar rats were fed a torula yeast-based se-deficient diet (se content, < 0.01 microg/g) for 28 d. during the experimental period, the diet of each group was supplemented with sodium selenate (0.17 microg se/g) for 0, 1, 2 ...200516521711
changes in glutathione peroxidase and tissue selenium concentrations of broilers after consuming a diet adequate in selenium.three experiments (exp) were conducted with commercial broilers to develop a low-se diet for comparing plasma glutathione peroxidase (pgpx3) concentrations and then to compare pgpx3 and plasma and tissue se concentrations in broilers fed this low-se diet after being supplemented with sodium selenite (ss) or se-enriched yeast (sy). with the exception of se, all diets were nutritionally adequate. the exp lasted from 0 to 20 or 22 d posthatching, and treatments were replicated with 6 to 8 pens of 6 ...200516156211
selenium bioavailability from buckwheat bran in rats fed a modified ain-93g torula yeast-based diet.selenium (se) is an essential nutrient for humans and animals. the se rda for adult humans is 55 mug/d; however, dietary amounts as high as 200 mug/d in the highly available form of selenomethionine in yeast were shown to reduce the incidence of certain cancers. a number of natural foods contain relatively high amounts of se; for the most part, however, the availability of food se for absorption and utilization is unknown. this experiment was conducted to determine the bioavailability of se from ...200516251622
field comparison of chemical attractants and traps for caribbean fruit fly (diptera: tephritidae) in florida citrus.field studies in citrus were conducted to compare the following as attractants for the caribbean fruit fly, anastrepha suspensa (loew): torula yeast-borax; propylene glycol (10%); a two-component lure consisting of ammonium acetate and putrescine; a two-component lure consisting of ammonium bicarbonate and putrescine; and a three-component lure consisting of ammonium bicarbonate, methylamine hydrochloride, and putrescine. various combinations of these attractants in glass mcphail, plastic mcphai ...200516334334
effect of the chemical form of supranutritional selenium on selenium load and selenoprotein activities in virgin, pregnant, and lactating rats.virgin, pregnant, and lactating rats were used to assess the influence of selenomethionine and selenocystine, fed at four to seven times the daily se requirement (supranutritional), on se load and selenoprotein activities. female sprague dawley rats (n = 48; age = 13 wk), reared on a low-se torula yeast diet, were assigned to one of three reproductive states (n = 16 per reproductive state) to occur simultaneously: virgin, pregnant, and lactating. once reproductive state was achieved, rats were f ...200515644515
effects of a supranutritional dose of selenate compared with selenite on insulin sensitivity in type ii diabetic dbdb mice.the present study was performed to examine the mechanism by which selenate ameliorates the insulin sensitivity in type ii diabetic dbdb mice. therefore, 21-adult female dbdb mice were randomly assigned to three experimental groups (0se, seiv and sevi) with seven animals per group. mice of group 0se were fed with a selenium-deficient diet (<0.02 mg se/kg) based on wheat and torula yeast for 8 weeks whereas the mice of groups seiv (selenite) and sevi (selenate) were fed with sodium selenite and so ...200515787978
high dietary intake of sodium selenite induces oxidative dna damage in rat liver.one mechanism for the cancer-chemopreventive effects of high selenium (se) intake is hypothesized to be antioxidant protection of dna. in this work we examine dna oxidation in whole animals as a function of dietary se intake and carcinogen administration. weanling male sprague-dawley rats were fed a basal, torula yeast-based, se-deficient diet supplemented with 0, 0.15, or 2.0 ppm se as sodium selenite for 10 wk. they were then injected with 0, 0.1, or 10 mg /kg body weight of the pro-oxidant ca ...200415203381
influence of adding borax and modifying ph on effectiveness of food attractants for melon fly (diptera: tephritidae).the melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae), is the most damaging pest of cucurbits in reunion island. the influence of adding borax and modifying ph on the effectiveness of different food attractants for both sexes of the melon fly is analyzed by a release-recapture method in field cages. adding borax to protein hydrolysates nulure and buminal strongly reduced their attractiveness for b. cucurbitae. acidification of 5% buminal solution (from ph 6 to ph 3) doubled it ...200415279302
the selenium requirement of the puppy.current selenium (se) recommendations for the puppy are based on extrapolation from other species (0.11 mg se/kg diet). the purpose of this study was to experimentally determine the se requirement in puppies. thirty beagle puppies (average = 8.8 weeks old) were utilized in a randomized complete block design with age, litter and gender used as blocking criteria. puppies were fed a low se (0.04 mg se/kg diet) torula yeast-based diet for 14 days (pre-test period) after which this same diet was supp ...200415387850
determination of the selenium requirement in kittens.the purpose of this study was to determine the selenium (se) requirement in kittens. thirty-six specific-pathogen-free kittens (9.8 weeks old) were utilized in a randomized complete block design to determine the se requirement in cats with gender and weight used as blocking criteria. kittens were fed a low se (0.02 mg/kg se) torula yeast-based diet for 5 weeks (pre-test) after which an amino acid-based diet (0.027 mg se/kg diet) was fed for 8 weeks (experimental period). six levels of se (0, 0.0 ...200314507414
the chemical form of selenium affects insulinomimetic properties of the trace element: investigations in type ii diabetic dbdb mice.the objective of the present study was to investigate the effects of oral selenate application in comparison to selenium deficiency and selenite treatment on the development of the diabetic status (glucose tolerance, insulin resistance and activities of glycolytic and gluconeogenic marker enzymes) in dbdb mice, representing a type ii diabetic animal model. therefore 21 adult male dbdb mice were assigned to 3 experimental groups of 7 animals each and put on a selenium deficient diet (< 0.03 mg/kg ...200314629895
nontarget insects captured in fruit fly (diptera: tephritidae) surveillance traps.traps baited with synthetic lures (ammonium acetate and putrescine) captured as many mexican fruit flies as the traditional torula yeast/borax slurry, but with far fewer (ratio 5:1) nontarget insects. ninety percent of the nontarget insects were dipterans. consequently, neither trap is efficacious against other citrus pests, which are mainly hemiptera or lepidoptera. although the nontarget catch is sometimes referred to as "trash," many nontarget insects are beneficials, including predators and ...200314977110
human urine and chicken feces as fruit fly (diptera: tephritidae) attractants for resource-poor fruit growers.we evaluated human urine and chicken feces, two naturally occurring, inexpensive, and readily available substances, as baits for the capture of anostrepha spp. (diptera: tephritidae) by using glass mcphail traps. two studies were performed simultaneously in a commercial mango orchard in veracruz, méxico. in the first study, we compared a 50% water dilution of human urine against hydrolyzed protein, both compounds at the fresh and 5-d-old stages, and water alone (control treatment). in the second ...200314994798
purine derivative excretion in dairy cows: endogenous excretion and the effect of exogenous nucleic acid supply.an experiment was conducted with dairy cows to study the partitioning of excreted purine derivatives between urine and milk and to quantify the endogenous contribution following the isotopic labeling of microbial purine bases. three lactating cows in their second lactation that had been cannulated in the rumen and the duodenum were fed a mixed diet (48:52, roughage/concentrate ratio) distributed in equal fractions every 2 h, and duodenal flow of purine bases was determined by the dual-phase mark ...200312741553
bioavailability of selenium in the defatted dark muscle of tuna.the content and bioavailability of selenium (se) in the dark muscle of tuna (dmt) were studied. fluorometric analysis showed that dmt contained 2.0-4.7 microg x g(-1) se. a large part of se of the dmt was recovered in the dry powder of the defatted fraction prepared by successive treatment with acetone and n-hexane/ethanol. on trypsin digestion of the defatted dmt, release of se paralleled that of nitrogen and about 70% of se was released within 4 h. male weanling ddy mice were fed a torula yeas ...200212443562
host selenium deficiency increases the severity of chronic inflammatory myopathy in trypanosoma cruzi-inoculated mice.weanling c3h/hen mice were fed either a torula yeast-based diet deficient in selenium (se) or the same diet supplemented with 0.2 ppm se as sodium selenite. after 4 wk of feeding, the mice were inoculated intraperitoneally with the ca-i strain (clone k98) of trypanosoma cruzi (tc). before inoculation, mean serum se levels were 430 versus 61 ng/ml in adequate and deficient mice, respectively. during the ascending phase of parasitemia, the se-deficient mice exhibited significantly higher levels of ...200212099423
structure of wyosine, the condensed tricyclic nucleoside of torula yeast phenylalanine transfer ribonucleic acid.large-scale isolation of the minor nucleoside wyosine of torula yeast trna(phe) was accomplished by a combination of enzymatic digestion and reversed-phase chromatography: the wyosine-containing nucleotide fraction, which was obtained by partial digestion of unfractionated trna (1 g) with nuclease p1, was concentrated by reversed-phase column chromatography followed by complete digestion with nuclease p1/alkaline phosphatase. the nucleoside mixture thus obtained was purified by reversed-phase hp ...200211964008
parameters of dietary selenium and vitamin e deficiency in growing rabbits.4 x 5 growing female rabbits (new zealand white) with an initial live weight of 610 +/- 62 g were fed a torula yeast based semisynthetic diet low in selenium (<0.03 mg/kg diet) and containing <2 mg alpha-tocopherol per kg (group i). group ii received a vitamin e supplementation of 150 mg alpha-tocopherylacetate per kg diet, whereas for group iii 0.40 mg se as na-selenite and for group iv both supplements were added. selenium status and parameters of tissue damage were analyzed after 10 weeks on ...200211878752
dietary selenite and azadeoxycytidine treatments affect dimethylhydrazine-induced aberrant crypt formation in rat colon and dna methylation in ht-29 cells.several observations implicate a role for altered dna methylation in cancer pathogenesis. the global level of dna methylation is generally lower; however, dna methyltransferase (dnmt1) activity is usually higher in tumor cells than in normal cells. the purpose of this study was to investigate whether the dnmt1 inhibitor, 5-aza-2'-deoxycytidine (aza-dc) would alter the effect of dietary selenium on the formation of aberrant crypts. weanling rats (n = 60) were fed three concentrations of selenium ...200211823593
bioavailability of selenium from raw or cured selenomethionine-enriched fillets of atlantic salmon (salmo salar) assessed in selenium-deficient rats.the bioavailability of se from raw and cured selenomethionine-enriched (se-enriched) salmon fillets was assessed in se-deficient male albino rats (mol: wist). a low-se torula yeast feed was supplemented with 0, 50, 100, 150 or 200 microg se/kg as sodium selenite or as se from raw or cured se-enriched salmon. the diets were fed to weanling rats for 10 and 30 d. bioavailability of se was assessed by metabolic balance, se accumulation in femur, muscle, liver and plasma, and induction of se-dependen ...200211895311
[on the identity of candida utilis and pichia jadinii yeast species].life cycles of candida utilis (henneberg) lodder et kreger-van rij imperfect yeast (13 strains) used in industry were the study subject. when the strains were mated, we detected one of the stages of sexual process--conjugation of cells of the opposite mating type. most of the studied cultures conjugated on the 2d-3d day. no ascospores were formed. haploidy and heterothallism of the studied c. utilis strains were confirmed by hybridization of auxotrophic mutants. based on pcr assay results, the y ...200211944341
selenium deficiency in fisher-344 rats decreases plasma and tissue homocysteine concentrations and alters plasma homocysteine and cysteine redox status.the purpose of the present study was to determine the effect of graded amounts of dietary selenium on plasma and tissue parameters of methionine metabolism including homocysteine. male weanling fisher-344 rats (n = 7-8/group) were fed a selenium-deficient, torula yeast-based diet, supplemented with 0 (selenium deficient), 0.02, 0.05 or 0.1 microg (adequate) selenium (as selenite)/g diet. after 61 d, plasma total homocysteine and cysteine were decreased (p < 0.0001) and glutathione increased (p < ...200212042420
assessment of selenium bioavailability from high-selenium spirulina subfractions in selenium-deficient rats.it was previously found that the bioavailability of se from se-rich spirulina (sesp) was lower than that from selenite or selenomethionine when fed to se-deficient rats. the present study examined the bioavailability of se from sesp subfractions: a pellet (p) issuing from the centrifugation of a suspension of broken sesp and a retentate (r) resulting from ultrafiltration of the supernatant through a 30 kda exclusion membrane. animals were fed a torula yeast based diet with no se (deficients) or ...200212059173
fermentability of grape must after inhibition with dimethyl dicarbonate (dmdc).dimethyl dicarbonate (dmdc) was added to grape must and to synthetic media and results showed that, at 20 degrees c, 150 mg.l(-)(1) dmdc completely inhibited the fermentation of a grape must that was previously inoculated with 10(6) cells.ml(-)(1) saccharomyces bayanus and saccharomyces uvarum. brettanomyces intermedius, candida guilliermondii, hansenula jadinii, hansenula petersonii, kloeckera apiculata, pichia membranaefaciens, and saccharomyces cerevisiae were inhibited by 250 mg.l(-)(1). can ...200212236685
selenium from selenium-rich spirulina is less bioavailable than selenium from sodium selenite and selenomethionine in selenium-deficient rats.the bioavailabilty of selenium (se) from selenium-rich spirulina (sesp) was assessed in se-deficient rats by measuring tissue se accumulation and glutathione peroxidase (gsh-px) activity. for 42 d, rats were subjected to dietary se depletion by consumption of a torula yeast (ty)-based diet with no se; controls were fed the same diet supplemented with 75 microg se/kg diet as sodium selenite. se-deficient rats were then repleted with se (75 microg/kg) by the addition of sodium selenite, selenometh ...200111533277
promotion of lipid oxidation by selenate and selenite and indicators of lipid peroxidation in the rat.the ain-93 reformulation of the ain-76a rodent diet includes a change in selenium supplement from sodium selenite to sodium selenate to reduce dietary lipid peroxidation. a change to selenate as the standard form of se in rat diets would render results from previous work using selenite less relevant for comparison with studies using the ain-93 formulation. to critically examine the rationale for the ain-93 recommendation, we prepared torula yeast basal diets patterned as closely as possible afte ...200111354350
in vitro function of s rnases in lycopersicon peruvianum.s rnases are products of the s locus that are expressed in the stylar tissue of lycopersicon peruvianum with the gametophytic self-incompatibility (si) system. two s rnases (s12 and sa) with rnase activity from the s12sa genotype of l. peruvianum were purified using gel filtration and cation-exchange chromatography. the molecular masses of the two rnases, s12 and sa, were 21 and 23.1 kda, respectively. the specific activities of s12 and sa for torula yeast rrna as a substrate were 8,500 and 6,00 ...200111804331
gene expression profiling of low selenium status in the mouse intestine: transcriptional activation of genes linked to dna damage, cell cycle control and oxidative stress.the essential trace mineral selenium (se) has been shown previously to inhibit intestinal, prostate, lung and liver tumor development and associated mortality in both experimental animals and humans. although se is likely to be one of the most powerful cancer chemopreventive agents in the human diet, its mechanism of action is unknown. to better understand the biological consequences of alterations in se status, the gene expression profile associated with low se status in the intestine of c57bl/ ...200111739862
[staining of the nucleus of torulopsis utilis]. 20014179329
dietary selenium and arsenic affect dna methylation in vitro in caco-2 cells and in vivo in rat liver and colon.selenium is an essential trace element for human health, and it has received considerable attention for its possible role as an anticarcinogenic agent. the purpose of the present study was to determine whether changes in the amount and the chemical form of selenium would affect dna methylation and whether this effect would be modified by arsenic. caco-2 cells, a human colon cancer cell line, were exposed to 0, 1 or 2 micromol supplemental selenite/l and 0, 1 or 2 micromol supplemental arsenite/l ...200011110844
selenium regulates expression in rat liver of genes for proteins involved in iron metabolism.suppression subtractive hybridization analysis in our laboratory recently revealed that transferrin mrna may be elevated in sedeficient rat liver. in this work, we compared expression in rat liver of genes for transferrin, transferrin receptor, ferritin light and heavy chains, and iron-regulatory proteins 1 and 2 in se adequacy and deficiency. weanling male sprague-dawley rats were fed torula yeast diets supplemented with 0 or 0.15 microg se/kg diet as sodium selenite for 15 wk. activity of cell ...200011049200
selenium from high selenium broccoli protects rats from colon cancer.colon cancer is the third most common newly diagnosed cancer in the united states and the third most common cause of cancer-related deaths. previous supplementation studies have demonstrated the efficacy of selenium (se) for prevention of colon cancer in humans. the metabolism of se depends on its chemical form, and studies have shown that the chemical form of se in broccoli does not accumulate in the body as fast as other forms of se and may be especially beneficial for prevention of cancer. in ...200010958840
determination of conspecificity of candida utilis and hansenula jadinii through dna reassociation. 2000575194
high levels of dietary vitamin e do not replace cellular glutathione peroxidase in protecting mice from acute oxidative stress.our objective was to determine whether high levels of dietary vitamin e replaced the protection of the se-dependent cellular glutathione peroxidase (gpx1) against paraquat- or diquat-induced acute oxidative stress in mice. two experiments were conducted using gpx1 knockout [gpx1(-/-)] mice and wild-type (wt) mice (n = 78/group). in experiment 1, mice were fed torula yeast-based, se-adequate (0.4 mg/kg as sodium selenite) diets + 0, 75, 750 or 7,500 mg all-rac-alpha-tocopheryl acetate for 5 wk be ...199910539768
an evaluation of the bioavailability of selenium in high-selenium yeast.the bioavailability of selenium (se) in high-se yeast (sey) was evaluated by measuring tissue se accumulation and glutathione peroxidase (gshpx) activity. for 4 weeks, 4-week-old male wistar rats were fed a torula yeast-based se-deficient diet (basal diet) or a diet supplemented with a graded level (0.04, 0.08, 0.16, and 0.32 microgram/g) of se as either sodium selenite or sey, which was obtained from two different sources. se supplementation did not influence growth, hematological values, or se ...199910360246
dietary selenium reduces the formation of aberrant crypts in rats administered 3,2'-dimethyl-4-aminobiphenyl.human epidemiologic studies suggest that low selenium status is associated with increased cancer risk and that selenium supplementation is associated with reduction in the incidence of several cancers, including colorectal cancer. aromatic and heterocyclic amine carcinogens are thought to be important in the etiology of human colorectal cancer, but no information is available on the effects of selenium on aromatic amine-induced colon cancer. in order to investigate this effect, aberrant crypt fo ...199910329505
chronic urinary tract infection due to candida utilis.an elderly male was seen at an outpatient urology clinic over a period of 3 years with repeat urine specimens containing 10(4) to 10(5) cfu of a "candida species, not c. albicans." the urine specimens were described as infected due to the presence of pyuria, but no antifungal therapy was administered. on two occasions, the patient presented to the emergency room and urine specimens were sent to the clinical microbiology laboratory. on both occasions, a yeast was isolated at concentrations of >10 ...19999986866
[priority of selenium incorporation into selenoproteins during selenium depletion in rats].male weanling wistar rats were fed with either a basal selenium deficient diet (a torula yeast based semisynthetic diet, containing se 0.01 mg/kg) or a selenium sufficient diet supplemented with se as na2seo3 (containing se 0.5 mg/kg). rats were killed after different weeks(0,1,2,4,8,12,15,17,19,20 and 24 respectively). their organs were taken to observe the kinetic change of selenium concentration, the activities of intracellular glutathione peroxidase (cgpx), extracellular glutathione peroxida ...199912712721
Displaying items 1 - 100 of 414