Publications

regioselective hydrolysis of acetates in the presence of different yeast strains.	the model compound, hexane-1,2-diol diacetate, was hydrolyzed in the presence of supernatant obtained after cultivation of 4 yeast strains: pichia jadinii, rhodotorula glutinis and yarrowia lipolytica kkp 379 and saccharomyces cerevisiae 102 to evaluate the type of catalysis. the regioselectivity of extracellular enzymes as a function of hydrolysis towards primary and secondary acetic acid ester groups was monitored. the enzymes secreted by p. jadinii, r. glutinis and y. lipolytica kkp 379 exhib ...	2014	25272729
asymmetric reduction of 4-hydroxy-2-butanone to (r)-1,3-butanediol with absolute stereochemical selectivity by a newly isolated strain of pichia jadinii.	in this study, a novel strain of pichia jadinii, hby61, capable of the biocatalysis of 4-hydroxy-2-butanone (4h2b) to (r)-1,3-bd was isolated. hby61 produced (r)-1,3-bd with high activity and absolute stereochemical selectivity (100 % e.e). glucose and beef extract were found to be the key factors governing the fermentation, and their optimal concentrations were determined to be 84.2 and 43.7 g/l, respectively. the optimal bioconversion conditions of 4h2b catalyzed by hby61 were ph 7.4, 30 °c, a ...	2014	25306883
rna-mediated cds-based nanostructures.	the colloidal method provides an important tool to synthesize different nanostructures and their assemblies. the coating of colloidal inorganic nanostructures by the biotemplate of similar dimension not only provides them the thermodynamic stability but also imparts the chemical features to grow in different dimensionalities and capabilities for molecular recognition. in this chapter we describe detailed methodology for the synthesis and characterization of cds nanoparticles templated by ribonuc ...	2015	25967063
purification and characterization of an extracellular uricase from a new isolate of sphingobacterium thalpophilum (vitpcb5).	an extracellular uricase producing bacterium (vitpcb5) was isolated from soil of the duck farm near chidambaram, tamilnadu, india and it was identified based on its 16s rrna as sphingobacterium thalpophilum. uric acid was used as an effective inducer. the enzyme kinetics was studied using uric acid as a substrate. the km and vmax for the enzyme was found to be 0.28mm and 0.92μm/minml, respectively. maximum uricase production was observed when lactose was used as a carbon source. among the nitrog ...	2015	26145833
the structure of the cyberlindnera jadinii genome and its relation to candida utilis analyzed by the occurrence of single nucleotide polymorphisms.	the yeast cyberlindnera jadinii is a close relative of candida utilis that is being used in the food and feed industries. here, we present the 12.7mb genome sequence of c. jadinii strain cbs 1600 generated by next generation sequencing. the deduced draft genome sequence consists of seven large scaffolds analogous to the seven largest chromosomes of c. utilis. an automated annotation of the c. jadinii genome identified 6147 protein-coding sequences. the level of ploidy for both genomes was analyz ...	2015	26150016
dietary selenium regulation of glutathione peroxidase mrna and other selenium-dependent parameters in male rats.	weanling male rats were fed a basal torula yeast diet (0.007 μg se/g diet) supplemented with graded levels of se (0 to 0.2 μg se/g diet as na2seo3) (three rats/group) to evaluate classical glutathione peroxidase (gpx1, gsh:h2o2, oxidoreductase, ec 1.11.1.9) mrna level as an indicator of intracellular se status. growth was followed throughout the dietary treatment and a number of se-dependent parameters including liver gpx1 mrna levels were determined after 33 days. growth was not impaired at any ...	1997	26273132
grape juice as a bait for anastrepha suspensa (diptera: tephritidae) and zaprionus indianus (diptera: drosophilidae).	in field tests conducted in south florida to test grape juice as a bait for the caribbean fruit fly, anastrepha suspensa loew, high numbers of zaprionus indianus gupta were captured in traps with aqueous grape juice. these experiments included comparisons of grape juice bait with established a. suspensa protein-based baits (ammonium acetate + putrescine lures, or torula yeast) or wine, a bait found previously to be attractive to z. indianus. effects of different preservatives (polypropylene glyc ...	2015	26470354
comprehensive analysis of the skin fungal microbiota of astronauts during a half-year stay at the international space station.	the international space station (iss) is a huge manned construct located approximately 400 km above the earth and is inhabited by astronauts performing space experiments. because the station is within a closed microgravity environment, the astronauts are subject to consistent stress. this study analyzed the temporal changes in the skin fungal microbiota of 10 astronauts using pyrosequencing and quantitative pcr assay before, during, and after their stay in the iss. lipophilic skin fungi, malasse ...	2016	26773135
enhanced treatment of tannery wastewater in an integrated multistage bioreactor (imbr) by the predominant bacterial strains enriched from marine sediments.	an innovative integrated multistage bioreactor (imbr) system, which was augmented with three predominant bacterial strains (lactobacillus paracasei cl1107, pichia jadinii cl1705, and serratia marcescens cl1502) isolated from marine sediments, was developed to treat real tannery wastewater without performing physicochemical pretreatment, with the potential to reduce the generation of waste sludge and odors. the performance of the imbr treatment system, with and without the inclusion of the predom ...	2016	26901723
genetic determinants for enhanced glycerol growth of saccharomyces cerevisiae.	the yeast saccharomyces cerevisiae generally shows a low natural capability to utilize glycerol as the sole source of carbon, particularly when synthetic medium is used and complex supplements are omitted. nevertheless, wild type isolates have been identified that show a moderate growth under these conditions. in the current study we made use of intraspecies diversity to identify targets suitable for reverse metabolic engineering of the non-growing laboratory strain cen.pk113-1a. a genome-wide g ...	2016	26971668
candida utilis and cyberlindnera (pichia) jadinii: yeast relatives with expanding applications.	the yeast candida utilis is used as a food additive and as a host for heterologous gene expression to produce various metabolites and proteins. reliable protocols for intracellular production of recombinant proteins are available for c. utilis and have now been expanded to secrete proteins into the growth medium or to achieve surface display by linkage to a cell wall protein. a recombinant c. utilis strain was recently shown to induce oral tolerance in a mouse model of multiple sclerosis suggest ...	2016	27357226
the comparative analysis of antioxidant and biological activity for the dendropanax morbifera lev. leaves extracted by different ethanol concentrations.	the different concentrations of ethanol (20-100%) and distilled water extract for dendropanax morbifera lev. leaves were evaluated to induce antioxidant and biological activity employed by variety of assays. the 20%, 80%, and 100% ethanol extract demonstrated the relatively higher activity, whereas distilled water, 40%, and 60% ethanol extracts exhibited the lower antioxidant and biological activity. especially, 80% ethanol extract showed the remarkably higher radical scavenging activity, reduci ...	2016	27592831
two-dimensional fluorescence difference spectroscopy to characterize nanoparticles and their interactions.	two dimensional fluorescence difference spectroscopy (2d fds) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths. comparing metal oxide nanoparticles revealed a unique spectral signature per material composition. 2d fds showed to be sensitive to changes in surface properties between zno nps synthesized by different methods. zno np loaded with glycol c ...	2016	27624316
evaluation of food lures for capture and monitoring of anastrepha fraterculus (diptera: tephritidae) on temperate fruit trees.	the anastrepha fraterculus (wiedemann) (diptera: tephritidae) is the main pest of fruit trees grown in temperate climates in the southern region of brazil. the objective of this work was to evaluate the efficiency of the major commercial food lures used in brazil for trapping and monitoring of a. fraterculus in plum, pear, and feijoa orchards. the assessed lures were hydrolyzed proteins of animal origin (ceratrap) and plant origin (bioanastrepha), torula yeast + borax (torula), and grape juice. ...	2017	28334322