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selenium deficiency and detoxication functions in the rat: effect of chronic dietary cadmium.male rats from moderately selenium-deficient dams were fed a torula yeast-based, selenium-deficient diet for 7 weeks, with or without added supplements of sodium selenite (0.2 ppm selenium) and cadmium chloride (50 ppm cadmium) in the drinking water. cadmium caused about 10% body-weight loss in selenium-deficient, as well as in supplemented rats. glutathione peroxidase activity in liver 105,000 g supernatant and in erythrocyte hemolysate from selenium-deficient rats was about 1% and 3%, respecti ...19854028975
effect of dietary selenium deficiency on incidence and size of 1,2-dimethylhydrazine-induced colon tumors in rats.torula yeast diets deficient (less than or equal to 0.02 ppm) in selenium (se) and a control diet supplemented with sodium selenite (0.1 ppm se), were fed to 52 male sprague-dawley rats per diet for 23 wk following weaning. 1,2-dimethylhydrazine (dmh) was administered (20 mg/kg body weight) in 20 weekly i.p. injections after 3 wk of feeding. after 23 wk, 67% of the rats fed the se-deficient diet had intestinal adenocarcinomas versus 63% on the 0.1 ppm se diets. diet also had no effect on the num ...19854032067
liver response to diet and estrogen in white leghorn and rhode island red chickens.experiments were conducted to compare the response of rhode island reds (rir) and white leghorns (wl) to estradiol (e2) administration and diets of different composition. in growing chickens injected with e2 for 7 days, rir accumulated significantly more hepatic lipid and had significantly less plasma lipid than wl. both rir and wl, injected for 4 days with e2, had lower hepatic lipids when fed a diet containing fish meal, alfalfa meal, and torula yeast (fay) than when fed a corn-soybean meal di ...19854039822
impaired ketone body metabolism in the selenium deficient rat. possible implications.male rats were fed a selenium-deficient torula yeast diet with or without 0.2 ppm selenium (as sodium selenite) in the drinking water. selenium deficiency caused a significant increase of urinary acetoacetate excretion in fed rats, and 24 or 48 hours of starvation enhanced this effect. two days of selenium supplementation decreased the amount of urinary acetoacetate and 3-hydroxybutyrate to 50% of the deficiency value, indicating an enzymatic impairment in the selenium-deficient rat. no selenium ...19854058313
differential effects of dietary selenium on hepatic and renal glutathione metabolism in the rat.studies were undertaken to determine whether or not dietary selenium (se), as sodium selenite, at suboptimal (unsupplemented torula yeast diets, 0.02 ppm se) or supplemented up to dietary excess, but nontoxic, levels (5.0 ppm se) could selectively modify hepatic and renal reduced glutathione (gsh) levels and the enzymes involved in gsh metabolism. male rats were provided torula yeast semipurified diets containing approximately 0.02 ppm se in the basal diet and supplements of 0.1, 2.0, or 5.0 ppm ...19854064933
effects of selenium supplementation on hepatocarcinogenesis in rats.four groups of weanling male wistar rats (groups a-d) received diethylnitrosamine (den, 40 ppm) in their drinking water for four weeks; after a recovery period of two weeks, they received (for the rest of the experiment) phenobarbital (pb, 500 ppm) added to a torula yeast-based diet containing 0.17 ppm of selenium. dietary selenium (2 ppm), as sodium selenite, was given to group b one week before and during den treatment, to group c one week before and during pb treatment, and to group d during ...19854070005
[initial research on the use of mixed corn grain and stalk silage in broiler fattening].the suitability of a corn and cob maize silage (ccm) as a component rich in energy in the fattening ration was investigated in a growth experiment with male broilers. the ccm silage with a dry matter content of 60.2% contained the following substances determining its value (per 1 kg dry matter of the feed): 102 g crude protein, 61 g crude fibre and 682 energetic feed units for chickens (efuc). ccm silage was either fed as a component of a feed mixture consisting of 70.5% ccm silage and 29.5% pro ...19854096637
[reductive catabolism of uracil in torulopsis utilis]. 19644157946
[catabolism of uracil in torulopsis utilis]. 19644157971
[stabilization of the properties of a test culture of torula utilis for determining the antifungal activity of antibiotics]. 20144175970
[staining of the nucleus of torulopsis utilis]. 20014179329
[on the effect of nicotinamide on the growth, the nitrogen metabolism and synthesis of lipids in endomycopsis vernalis and torulopsis utilis]. 19664230276
dietary induction of mulberry heart disease and hepatosis dietetica in pigs. i. nutritional aspects.two trials were conducted, involving 48 yorkshire specific pathogen-free pigs, three to four weeks old, to investigate diets which would result in a high incidence of deaths from mulberry heart disease and hepatosis dietetica in pigs. diets based on ground corn with torula yeast resulted in a high incidence of death. protein supplements of dried skim milk or soybean meal with corn did not induce a high incidence of death. diets supplemented with torula yeast had the lowest selenium concentration ...19724263917
effect of supplemental vitamin e and selenium in high moisture corn diets on the incidence of mulberry heart disease and hepatosis dietetica in pigs.forty-eight intact male pigs were used to investigate the influence of source of protein supplement, corn moisture content, and supplemental vitamin e-selenium survived the cidence of mulberry heart disease, hepatosis dietetica and associated lesions. pigs fed soybean meal-high moisture corn diets survived fewer days than pigs fed the other diets. none of the pigs fed torula yeast-dry corn or soybean meal-high moisture corn without supplemental vitamin e-selenium survived the 13 week trial. supp ...19724263921
vitamin e, selenium and methionine supplementation of dystrophogenic diets for pigs.forty-eight weanling s.p.f. yorshire pigs were used to study the influence of supplemental vitamin e (25 iu per kg of diet) selenium (0.5 ppm in diet) and methionine (0.1% in diet) on the incidence of hepatosis dietetica and mulberry heart disease when fed a torula yeast-corn diet. vitamin e and/or selenium increased pig survival. supplemental selenium resulted in increased liver selenium concentrations. no hepatosis dietetica was observed in any of the pigs. the addition of vitamin e and/or sel ...19724263922
the primary structure of valine-i transfer ribonucleic acid from torulopsis utilis. 19684299379
the primary structure of valine-i transfer ribonucleic acid from torulopsis utilis. i. complete digestion with pancreatic ribonuclease and ribonuclease t1. 19684305011
nucleotide sequence of tyrosine transfer rna from torulopsis utilis. 19694308683
a comparison of mitochondria from torulopsis utilis grown in continuous culture with glycerol, iron, ammonium, magnesium or phosphate as the growth-limiting nutrient.1. mitochondria prepared from torulopsis utilis grown in a chemostat with iron-limited growth were found to lack energy conservation but not electron flow in that segment of the respiratory chain leading from intramitochondrial nadh to the cytochromes [i.e. the site 1 segment (lehninger, 1964)]. 2. site 1 energy conservation was present in mitochondria prepared from cells grown under conditions of limitation by glycerol, ammonium and magnesium. phosphate-limited growth resulted in mitochondrial ...19714331254
non-haem iron and the dissociation of piericidin a sensitivity from site 1 energy conservation in mitochondria from torulopsis utilis.1. the aerobic incubation of iron-deficient torulopsis utilis cells for 12h under non-growing conditions results in the recovery by mitochondria of the previously absent site 1 energy conservation and sensitivity to piericidin a. 2. the recovery of piericidin a sensitivity but not site 1 is prevented by the presence of cycloheximide (100mug/ml) in the medium used for aerobic incubation of the cells. rotenone sensitivity behaved similarly. 3. chloramphenicol, erythromycin and tetracycline were wi ...19714331255
complete digestion of tyrosine transfer ribonucleic acid from torulopsis utilis with pancreatic and t 1 ribonucleases. 19724341741
[different properties of nicotinamide desamidases from torulopsis utilis and endomycopsis vernalis]. 19704394573
effect of sulphate-limited growth on mitochondrial electron transfer and energy conservation between reduced nicotinamide-adenine dinucleotide and the cytochromes in torulopsis utilis.1. conditions have been established for the sulphate-limited growth of torulopsis utilis in continuous culture. 2. mitochondria prepared from sulphate-limited cells lack both piericidin a sensitivity and the first energy-conservation site (site 1). sensitivity to antimycin a or cyanide and the second and third energy-conservation sites were apparently unaffected by sulphate-limited growth. 3. aerobic incubation for 8h of sulphate-limited cells with a low concentration of sulphate (50mum or less) ...19714399517
spectroscopic studies of flavoproteins and non-haem iron proteins of submitochondrial particles of torulopsis utilis modified by iron- and sulphate-limited growth in continuous culture.1. a spectroscopic resolution has been made of the components contributing to the ;iron-flavoprotein' trough extending from 450 to 520nm in the reduced-minus-oxidized difference spectrum of submitochondrial particles of torulopsis utilis. 2. seven components were identified other than cytochrome b, ubiquinone and succinate dehydrogenase. on the basis of the effects of iron- and sulphate-limited growth of cells on their subsequently derived electron-transport particles, and also by consideration ...19714399518
the purification and properties of the respiratory-chain reduced nicotinamide--adenine dinucleotide dehydrogenase of torulopsis utilis.1. an nadh-ferricyanide reductase activity has been isolated from the respiratory chain of torulopsis utilis by using detergents. the isolated enzyme contains non-haem iron, acid-labile sulphide and fmn in the molar proportions 27.5:28.4:1. the preparation is free of fad and largely free of cytochrome. 2. the enzyme catalyses ferricyanide reduction by nadph at about 1% of the rate with nadh, and reacts poorly with acceptors other than ferricyanide. the rates of reduction of some acceptors are, a ...19714399788
[stimulation of growth in torulopsis utilis (candida utilis) by various vitamin- and growth-factor sources in solutions containing sucrose]. 19744471907
structure and function of 5s ribosomal ribonucleic acid from torulopsis utilis. i. purification and complete digestion with pancreatic ribonuclease a and ribonuclease t. 19744476732
structure and function of 5s ribosomal ribonucleic acid from torulopsis utilis. ii. partial digestion with ribonucleases and derivation of the complete sequence. 19744476733
the detection of delta-aminolaevulinic acid synthetase in anaerobically grown torulopsis utilis. 19724542156
physico-chemical studies on conformation of a complex reconstituted from half molecules of torulopsis utilis tyrosine transfer ribonucleic acid. 19724567663
nucleotide sequence of 5 s rna from torulopsis utilis. 19744604831
partial digestion with ribonuclease t 1 and derivation of the complete sequence of tyrosine transfer ribonucleic acid from torulopsis utilis. 19724672191
atp: urea amidolyase (adp) in torulopsis utilis cultivated on ammonium sulphate as the sole nitrogen source. 19724672251
mechanism of urea decomposition by urease-less torulopsis utilis. 19724677126
selenium retention in tissues and sperm of rats fed a torula yeast diet. 19734682443
desaturation of oleyl phospholipid to linoleyl phospholipid in torulopsis utilis. 19734735345
the primary structure of alanine transfer ribonucleic acid 1 from torulopsis utilis. i. complete digestion with pancreatic ribonuclease and ribonuclease t1. 19734796875
the primary structure of alanine transfer ribonucleic acid 1 from torulopsis utilis. ii. partial digestion with ribonuclease t1 and derivation of the complete sequence. 19734796876
300 mhz pmr studies on the conformation of the hexanucleotide, 2'omegpapapupappsi, from the anticodon loop of torula yeast trnaphe. 19744831072
biochemical applications of continuous culture: energy-conservation mechanisms in torulopsis utilis. 19704920880
chemical modification of pseudouridylic acid in isoeleucine transfer ribonucleic acid from torulopsis utilis with a radioactive carbodiimide. 19725064241
chemical modification of pseudouridylic acid in valine transfer ribonucleic acid i from torulopsis utilis with a radioactive carbodiimide. 19725064995
utilisation of urea by urease-free organisms torulopsis utilis. 19725065942
acquisition and loss of rotenone sensitivity in torulopsis utilis. 19715106112
growth yields of torulopsis utilis grown in continuous culture with glycerol or iron as the growth-limiting nutrient. 19715166589
use of torulopsis utilis and invert sugar in streptomycin fermentation. 19705464095
fractionation of torulopsis utilis transfer ribonucleic acid. 3. different transfer rna's with an identical amino acid-accepting specificity. 19675586496
fractionation of transfer ribonucleic acids from torulopsis utilis. iv. purification of the alanine, valine and isoleucine transfer ribonucleic acids by column chromatography. 19675588798
tissue selenium levels during the development of dietary liver necrosis in rats fed torula yeast diets. 19685665646
the primary structure of valine-i transfer ribonucleic acid from torulopsis utilis. ii. partial digestion with ribonuclease t1 and derivation of the complete sequence. 19685718549
fractionation of transfer ribonucleic acids from torulopsis utilis. v. purification of the phenylalanine, lysine and histidine transfer ribonucleic acids. 19685749312
analyses of pancreatic ribonuclease digests of torulopsis utilis transfer ribonucleic acid's specific for alanine, valine and several other amino acids. 19695814320
nucleotide sequence of isoleucine transfer rna from torulopsis utilis. 19695815243
the primary structure of isoleucine transfer ribonucleic acid from torulopsis utilis. complete digestion with ribonucleases and construction of model of its secondary structure. 19695817084
[effect of torula utilis and candida arborea on the respiratory tract in workers employed in the production of yeast]. 19655853137
effect of dilution rate and growth-limiting substrate on the metabolic activity of torula utilis cultures. 19655883694
[study of the cytochromic system of torula utilis by the distribution of the reduction rates of the chain links during steady state]. 20145887015
continuous culture of torulopsis utilis: a kinetic study of oxygen limited growth. 19665969749
[studies on the metabolism of urea in microorganisms. v. the specific activity of the ornithine cycle enzymes in endomycopsis vernalis and torulopsis utilis in relation to the n source]. 19665990743
structure of the 5-s ribosomal rna gene and its adjacent regions in torulopsis utilis.a dna segment spanning one repeating unit of ribosomal rna(rrna) genes in a yeast strain, torulopsis utilis, has been cloned on a bacterial plasmid pbr322. the size of the cloned segment was about mr = 8.0 x 10(6). all of the genes for the four species of rrna were linked on it, and there was a long spacer between the 5-s and 18-s rrna coding regions. the nucleotide sequences of the regions flanking the 5-s rrna gene were determined and compared with those in the corresponding regions of sacharo ...19806254757
nucleotide sequences of the 5s ribosomal rna genes and their adjacent regions in schizosaccharomyces pombe.the organization of 5s ribosomal rna (rrna) genes in the genome of schizosaccharomyces pombe has been investigated by restriction and hybridization analyses. the 5s rrna genes were not linked to the other three species of rrna genes which formed a repeating unit of 6.9 megadaltons, but located in other regions surrounded by heterogeneous sequences. the 5s rrna gene organization in s. pombe is therefore different from those in other yeasts; saccharomyces cerevisiae and torulopsis utilis. four res ...19816275361
bioavailability to rats of selenium in various tuna and wheat products.bioavailability of selenium (se) in tuna and wheat at various stages of processing was studied in rats. the protein source of the rat diets was torula yeast with se supplied by either raw, precooked or canned tuna, or whole wheat flour, whole wheat bread or bran. sodium selenite was used as the standard. each se source was fed at three levels: 0.05, 0.10 and 0.15 ppm. by using increase in glutathione peroxidase (gsh-px) activity in liver, kidney and whole blood as an indicator of bioavailability ...19836296340
sequence of trna ile iau from brewer's yeast.the nucleotide sequence of trnaile from brewer's yeast saccharomyces cerevisiae was determined. its primary structure is pg-g-u-c-u-c-u-u-m1g-m2g- c-c-c-a-g-d-d-g-g-d-d-a-a-g-g-c-a-c-c-g-u-g-c-u-i-a-u-t6 a-a-c-g-c-g-g-g-ga-d-m5 c-a-g-c-g-g-t-psi-c-g-m1 a-u-c-c-c-g-c-u-a-g-a-g-a-c-c-a-c-c-a. its anticodon is i-a-u. it should therefore recognize the three isoleucine codons and is for this reason probably the only isoacceptor trna for isoleucine in brewer's yeast. it presents a large homology with ...19846370253
glucose-6-phosphate dehydrogenase. a transferred nuclear overhauser enhancement study of nadp+ conformations in enzyme-coenzyme binary complexes.the conformation of nadp+ in glucose-6-phosphate-dehydrogenase--nadp+ binary complexes has been investigated using proton-proton transferred nuclear overhauser enhancement measurements to determine interproton distance ratios between bound nadp+ protons. the enzymes from saccharomyces cerevisiae (brewer's yeast and baker's yeast) and hansenula jadinii (candida utilis, torula utilis) form binary complexes with nadp+ in which the glycosidic bond of the adenine moiety is in the anti conformation wh ...19846499847
effect of dietary composition and estradiol implants on hepatic microsomal mixed function oxidase and lipid deposition in growing chicks.three experiments were conducted to investigate the effect of diet and estradiol (e2) administration on hepatic microsomal mixed function oxidase (mfo) activity, e2 metabolism, and liver lipid content in male broiler chicks. broiler chicks (3 weeks of age) were fed either a corn-soybean (cs) diet or a diet containing fish meal, alfalfa meal, and torula yeast (fay) for 19 days in experiments 1 and 3 and for 14 days in experiment 2, respectively. half of the chicks were implanted with tubes contai ...19846514666
effect of supplemental selenium on pancreatic function and nutrient digestibility in the pig.the effect of se deficiency on pancreatic digestive enzyme activity and nutrient digestibility was studied by using pigs weaned at 3 wk of age from sows fed a diet deficient in se and low in vitamin e. pigs were fed a torula yeast diet supplemented with 100 iu dl-alpha-tocopheryl acetate/kg of diet. treatments were levels of supplemental se of 0, .025, .050, .075 or .100 ppm. apparent digestibility was determined at the end of the second and fourth week. digestive enzyme activity, glutathione pe ...19846608513
factors influencing the antitumorigenic properties of selenium in mice.sodium selenite (na2seo3) was administered at 2.0 micrograms selenium (se) to swiss icr mice six times over a 9-day period by intraperitoneal (i.p.) injection or by gastric gavage. survival time was significantly increased in ehrlich ascites tumor (eat)-bearing mice by 170 and 20%, respectively, compared to controls. in two separate studies, 5.0 micrograms se as na2seo3 or selenodiglutathione (gssesg) administered i.p. was more effective in inhibiting eat propagation in mice than either untreate ...19836631533
a modified nucleoside located in the anticodon of proline trna from torulopsis utilis is 5-carbamoylmethyluridine.a modified nucleoside has been isolated from the first position of the anticodon of torulopsis utilis trnapro. it was identified to be an uridine derivative, 5-carbamoylmethyluridine from analyses of its uv, 1h-nmr, and secondary ion mass spectra.19836686673
responses in calcium and phosphorus metabolism and hepatic lipid deposition among estrogenized chicks fed various dietary ingredients.the purpose of this study was to determine whether diet composition would influence calcium and phosphorus metabolism in chicks administered estrogen. at 1 day of age, broiler chicks were fed either a corn-soybean meal diet (cs), or an isoenergetic and isonitrogenous diet containing 5% fish meal, 5% alfalfa meal and 10% torula yeast (fay). at 21 days equivalent numbers were implanted with one of two lengths of silastic tubing containing estradiol dipropionate, while the remaining birds served as ...19846699740
effect of selenium on performance, serum selenium concentration and glutathione peroxidase activity in pigs.pigs from sows fed a diet deficient in se and low in vitamin e were fed a torula yeast diet supplemented with 100 iu dl-alpha-tocopheryl acetate/kg of diet. dietary treatments were levels of supplemental se of 0, .025, .050, .075 or .100 ppm. some death loss occurred in pigs receiving no supplemental se at approximately 5 wk of age. autopsy revealed liver and heart lesions typical of vitamin e-se deficiency. selenium supplement had no significant effect on average daily gain, feed intake or gain ...19846706871
diet composition, environmental temperature, and exogenous estradiol effects on hepatic lipid deposition in growing chicks.the interrelationships among dietary composition, environmental temperature, and level of estradiol (e2) administration on hepatic lipid deposition in chicks were studied. two levels of e2 were injected at three intervals over a 4-day period, in 3-week-old male leghorn chicks fed either a corn-soybean meal (cs) diet or a diet containing fish meal (fm) after 2 days fasting. the chicks were subjected to temperature ranges of 15 to 24 c (low) or 24 to 35 c (high) at 2 weeks of age. the e2 (in silas ...19846718303
effect of fractions of fish meal and hepatic lipid deposition in estrogenized chicks.three experiments were conducted for the purpose of determining the effect of feeding isoenergetic diets with and without fish meal or its fractions on hepatic lipid deposition in estrogenized chicks. the chicks were fasted for 48 hr before being fed the experimental diets and were injected with estradiol three times during the experiment. after 4 days, hepatic lipid content was determined. in the first experiment, the quantity of hepatic lipid deposited per unit of liver and per 100 g body weig ...19846728789
effects of dietary selenium and vitamin e on hepatic mixed-function oxidase activities and in vivo covalent binding of aflatoxin b1 in rats.male weanling fischer-344 rats were fed a selenium (se)-vitamin e (ve) deficient torula yeast basal diet or that diet supplemented with a graded levels of se (0.2-6.0 ppm as na2seo3) or ve (100 iu/kg as all-rac-2-tocopheryl acetate), or both, for 4 or 6 weeks. se deficiency and excess (6.0 ppm) markedly depressed in vivo covalent binding of aflatoxin (afb1) to macromolecules in livers of rats killed 2 hours after an i.p. dose of 1 mg/kg tritiated afb1. ve supplementation had no effect. prior phe ...19826799626
effects of chlorinated bisphenols on torula yeast glucose-6-phosphate dehydrogenase.chlorinated bisphenol antibacterial and antifungal agents are potent inhibitors of torula yeast glucose-6-phosphate dehydrogenase (g6pd). several compounds were tested, including hexachlorophene [hcp; 2,2'-methylenebis(3,4,6-trichlorophenol)]; 2,2'-oxybis(tetrachlorophenol); 2',4-dihydroxy-2,3,3',5,5',6-hexachlorodiphenylmethane; 2,2'-methylenebis(3,4-dichlorophenol) (3,4-tcp); bithionol [2,2'-thiobis(4,6-dichlorophenol)]; 2,2'-methylenebis(3,5-dichlorophenol); 2,2'-dihydroxy-3,3',5,6,6'-pentach ...19816802984
effect of short-term feeding of sodium selenite on 7,12-dimethylbenz(a)anthracene-induced mammary carcinogenesis in the rat.the inhibitory activity of short-term feeding of one of four concentrations of dietary selenium against the induction of mammary gland carcinomas by 7,12-dimethylbenz(a)anthracene (dmba) was studied in female sprague-dawley rats. when 28 days old, the animals were placed on a torula yeast diet formulation which contained, by analysis, either 0.05, 0.15, 1.05, or 2.06 microgram of selenium, as sodium selenite, per g of diet. mammary cancer was induced by a single p.o. administration of either 7.5 ...19826814747
laboratory model with chicks for assay of nutritional factors affecting hepatic lipid accumulation in laying hens.a laboratory model with chicks is described that is suggested as an assay for dietary factors affecting liver lipid accumulation in laying hens. the model utilizes growing white leghorn male chicks injected with estrogen. diets to be tested were fed ad libitum for 4 days following 2 days starvation. liver lipid deposition was increased with increasing level of estradiol injected. lipid deposition in livers of estrogenized chicks fed a diet containing fish meal, alfalfa meal, and torula yeast (fa ...19836828406
tolerance of diets deficient or excessive in selenium by syrian hamsters.syrian hamsters were fed torula yeast (ty) diets with 8 selenium (se) supplement levels (0.0-10.0 ppm se as sodium selenite) or casein (c) diets with 5 supplement levels (0.0-5.0 ppm se as sodium selenite) for 25 weeks. whole blood se, plasma glutathione peroxidase (gsh-px) activity and erythrocyte gsh-px activity were measured after 5, 10, 15 and 25 weeks. at 25 weeks hematology was examined and tissue samples analyzed for se and evaluated for histopathological lesions. while survival was not i ...19836847126
roles of selenium and sulfur-containing amino acids in protection against oxygen toxicity.tolerance and adaptation to hyperoxia have been correlated with increases in antioxidant enzymes. this study evaluated whether selenium deficiency would prevent an increase in glutathione peroxidase (gshpx), a selenium-containing enzyme, during oxygen exposure, and, thus, inhibit adaptation. because the torula yeast-based diet, which was used to produce selenium deficiency, was also deficient in cysteine and methionine, the effects of these deficiencies were also evaluated. when rats were expose ...19836876743
toxic effects of dietary selenium in the syrian hamster.selenium (se) toxicity and utilization was evaluated in hamsters fed casein- and torula yeast-based diets. 4-week-old hamsters received semipurified diets for 21 days. in experiment i diets were supplemented with either 0.25, 10, 20, 40 or 80 ppm se as sodium selenite (ss) and in experiment ii diets were supplemented with 0.1, 5.0 or 10.0 ppm as ss or selenomethionine (sm). blood and tissue se concentrations and glutathione peroxidase (gsh-px) activity were measured at the termination of the fee ...19836881915
the nucleotide sequence and codon recognition of proline trna from torulopsis utilis.proline trna from torulopsis (candida) utilis was purified by chromatography on columns of deae-sephadex a-50 at ph 7.6 and 4.0, deae-sephadex a-50 at ph 6.0, and rpc-5 at ph 4.0. this trna was sequenced by combining conventional chromatographic methods with rapid read-out chemical and enzymatic gel sequencing methods. the trna consists of 75 nucleotides and had the following sequence: pg-g-c-cm-g-c-g-u-m1g-g-u-c-psi-a-g-d-g-g-d-a-u-g-a-u-a-c-u-c-g-c-u-u-n-g-g-m1g -psi-g-psi-g-a-g-u-g-m7g-d-m5c- ...19826920386
beneficial effect of chromium-rich yeast on glucose tolerance and blood lipids in elderly subjects.twenty-four volunteers, mean age 78, including eight mildly non-insulin-dependent diabetics, were randomly allocated to one of two groups and were fed (daily for 8 wk) 9 g of either chromium-rich brewers' yeast (experimental) or chromium-poor torula yeast (control). before and after yeast supplementation, the serum glucose and insulin response to 100 g oral glucose was measured at 30 min intervals for 2 h. fasting serum cholesterol, total lipids, and triglycerides were also determined. in the to ...19807000589
interaction of initiator met-trnarmet (escherichia coli) and gly-trnaigly (staphylococcus epidermidis) with bacterial elongation factor tu:gtp complex.jekowsky et al. reported recently that elongation factor tu:gtp complex from escherichia coli protected aminoacyl-trna from digestion by pancreatic rnase (i). on the basis of their finding, we have developed the "rnase-resistance assay" for determination of the dissociation constant of aminoacyl-trna from aminoacyl-trna:ef-tu:gtp complex. by the use of this sensitive assay, the dissociation constants were estimated to be 3.6 x 10(-7) m for ala-trna1ala (torulopsis utilis), 7.9 x 10(-8) m for phe ...19817024261
effect of estrogen implants on hepatic lipid deposition in chicks fed different isonitrogenous and isocaloric diets.two experiments were conducted to determine if differences in response of livers to estradiol administration in male broiler chicks would be observed if fed isonitrogenous and isocaloric diets of different composition. a polymethylsiloxane tube filled with estradiol dipropionate was implanted under the neck skin to ensure a constant release of hormone. different tube sizes were implanted in two- to three-week-old chicks fed a basal corn-soybean meal (cs) diet or a diet containing fish meal, alfa ...19827054466
plasma estradiol, thyroid hormones, and liver lipid content in laying hens fed different isocaloric diets.two experiments were conducted with laying hens fed a corn-soy basal diet or diets containing fish meal, distillers dried grains with solubles (ddgs) or torula yeast formulated to be isocaloric and isonitrogenous with the basal diet. one half of the hens were kept at a temperature range of 13-24 degrees and the other half at 24-35 degrees for 49 days in experiment 2. liver lipid content was significantly lower in hens fed ddgs (experiment 1) and ddgs or fish meal (experiment 2) than in hens fed ...19827057268
some metabolic interrelationships between toxic levels of cadmium and nontoxic levels of selenium fed to rats.male rats were fed vitamin e-adequate, torula yeast-based diets for 30 days to assess the influence of dietary selenium (0, 0.1, or 1.0 ppm) on the toxicity of dietary cadmium (0, 30, or 60 ppm). at all selenium levels, increased cadmium intake depressed feed consumption, reduced feed efficiency and lowered body weight gain. in liver, concentrations of cadmium and zinc increased, and iron concentration decreased with increased intake of cadmium. dietary selenium did not affect concentrations of ...19827077426
comparative effects of sodium selenite and selenomethionine upon nutritional muscular dystrophy, selenium-dependent glutathione peroxidase, and tissue selenium concentrations of turkey poults.day-old poults from hens depleted of se were fed low-se basal diets (containing corn, soybean meal, and torula yeast but no added vitamin e) with graded levels of se supplied by na2seo3 or seleno-dl-methionine for 28 and 35 days in experiments 1 and 2, respectively. adding .04 ppm se to the basal diet significantly increased body weight and reduced both the incidence of gizzard myopathy and plasma glutamic-oxaloacetic transaminase (pgot) activity. further plasma se and se-dependent glutathione p ...19827088800
selenium in the testis of the rat: studies on its regulation and its importance for the organism.in male rats, fed for 10 weeks on a torula yeast-based, low selenium and low vitamin e diet, the selenium level and the glutathione peroxidase activity in the blood and in several tissues decreased by 50 to 98% compared with animals that received the same basal diet supplemented with 0.25 mg se/kg sodium selenite. in the testes, however, the selenium content did not differ from that of the control animals. despite the low selenium levels in the extragonadal tissues and their increased requiremen ...19827108639
nutritional availability to rats of selenium in tuna, beef kidney, and wheat.weanling male rats were fed a selenium (se)-deficient 40% torula yeast diet for 4 weeks and were then continued on depletion for another 4 weeks or were repleted with 0.1, 0.2, or 0.3 ppm se as sodium selenite or 0.2 ppm se as tuna, beef kidney, or high-se wheat. there were no significant differences in young red blood cell se, unfractionated red blood cell se, or liver se levels in groups fed diets containing 0.2 ppm se. glutathione peroxidase (gsh-px) activity in young red blood cells was lowe ...19817310542
selenium and reproductive function in boars fed a low selenium diet.a study was conducted with 24 crossbred boars (77.5 +/- 2.8 days of age) to determine the effects of low se status on various spermatozoal characteristics and on se concentration in semen, serum and primary and accessory reproductive tissues. all boars were fed a low se diet (cornstarch and torula yeast) ad libitum. twelve boars were injected every 14 +/- 1 days with sodium selenite (.33 mg se/kg body weight) and 12 served as saline-treated controls (low se status). at 210 +/- 5 days of age, six ...19817319974
effect of acriflavine on the hexokinase isozyme pattern of a yeast, hansenula jadinii.dried cells of a yeast, hansenula jadinii, that had been cultured aerobically with acriflavine, contained three hexokinase isozymes and metabolized glucose at 0.6 m to produce atp to phosphorylate nucleotides in the presence of a high concentration of phosphate. dried cells cultured aerobically without acriflavine contained two hexokinase isozymes and could not metabolize glucose under the same conditions. two of the isozymes of the yeast cultured with acriflavine were similar to isozymes of the ...19807388034
respiratory pentane: a measure of in vivo lipid peroxidation applied to rats fed diets varying in polyunsaturated fats, vitamin e, and selenium and exposed to nitrogen dioxide.pentane is one decomposition product of omega 6-unsaturated lipid hydroperoxides. the measurement of respiratory pentane is one of the most sensitive in vivo tests of lipid peroxidation. this measurement was applied to test the ability of a low level, short-term exposure of rats of nitrogen dioxide to induce lipid peroxidation. when exposed to 4.48 ppm nitrogen dioxide for 60 min, no increase in pentane production was detected. for 10 weeks prior to exposure, the rats were fed torula yeast-based ...19807423553
dietary selenium and age-related susceptibility of rat erythrocytes to oxidative damage.the effects of age and dietary selenium on cellular susceptibility to oxidative stress were studied in rat erythrocytes. young (26 or 40 days) and mature adult (11 or 15 months) male rats were fed a torula yeast-based low selenium and low vitamin e diet supplemented with either 0, 1.0 to 2.0 ppm selenium (as sodium selenite) for 5 weeks. the rates of spontaneous hemolysis amd methemoglobin formation and levels of glutathione (gsh), glucose-6-phosphate (g6p) dehydrogenase, but not of gsh peroxida ...19807441374
effect of dietary methionine on the biopotency of selenite and selenomethionine in the rat.the effect of dietary methionine (met) on the biopotency of selenium (se) from selenite and selenomethionine (se-met) was studied in rats fed a 30% torula yeast-based diet containing 0.24% met and less than 0.02 ppm se. biopotency was quantitated by assaying liver, plasma and heart for the glutathione peroxidase (gsh-px) increase elicited by a given level of se fed for 1 week to se-deficient rats. at dietary se levels up to 0.5 ppm se, the level of dietary met supplementation did not alter selen ...19817452376
effect of ingestion of 6-propyl-2-thiouracil on biochemical indices of selenium status in rats.biochemical indices of selenium (se) deficiency (liver se content and glutathione peroxidase (gshpx) activity, and urinary ketone bodies during fasting) were measured in se-deficient or se-sufficient control rats with or without ingestion of 6-propyl-2-thiouracil (ptu). male weanling wistar rats (50 to 60 g) were fed on a torula yeast-based se-deficient diet (se content, < 0.01 microgram/g), or on the same diet supplemented with sodium selenite (0.1 microgram se/g). the rats were given 0.05% ptu ...19957595971
mineral status in selenium-deficient rats compared to selenium-sufficient rats fed vitamin-free casein-based or torula yeast-based diet.to clarify the mineral status in selenium (se)-deficient rats fed a vitamin-free casein (vfc)-based or torula yeast (ty)-based diet, 24 weanling male wistar rats were divided into 4 groups fed diets using vfc or ty as the protein source and containing se at sufficient (0.5 microgram/g, +se) or deficient (0.019 microgram/g for vfc-based and < 0.005 microgram/g for ty-based diets, -se) level for 8 wk. ty supplied a larger amount of extra minerals (na, k, ca, mg, fe, mn, zn, and cu) except se than ...19937688535
selenium deficiency as a cause of overload of iron and unbalanced distribution of other minerals.previous studies have shown that there are hematological abnormalities in selenium (se)-deficient animals. this study examined the effects of se deficiency on various minerals in serum and other tissues of male wistar rats. the animals were given free access to either torula yeast-based se-deficient (sed) diet or se-adequate (sea) (containing 0.1 mg se/kg diet as sodium selenite) diet. blood was sampled after 12 and 24 weeks, and the rats were killed after 24 weeks, for the analysis of minerals ...19957766029
the phylogenetic relationships of pichia jadinii, formerly classified in the genus hansenula, and related species based on the partial sequences of 18s and 26s ribosomal rnas (saccharomycetaceae).we analyzed 18s and 26s rrna partial base sequences [positions 1451-1618 (168 bases) of 18s rrna and positions 1611-1835 (225 bases) and 493-622 (130 bases) of 26s rrna] of a total of three strains of pichia jadinii and candida utilis. the three strains had identical base sequences with the type strain of p. jadinii (ifo 0987) in the 18s rrna partial base sequencings. in the 26s rrna partial base sequencings, there were partial base sequences similar to each other (1-0 base difference and 87-95 ...19957766195
selenium from beef is highly bioavailable as assessed by liver glutathione peroxidase (ec 1.11.1.9) activity and tissue selenium.the bioavailability of se from ground beef has been previously found in this laboratory to be greater than that of selenite or selenate when fed to female fischer 344 rats. in the present study we examined the bioavailability of se from various commercial portions of beef, the liver, striploin, round, shoulder and brisket. all beef was cooked, freeze-dried, finely powdered and mixed with the other dietary ingredients. the experimental diets were fed to the weanling fischer 344 rats which had bee ...19947827008
selenium deficiency alters thyroid hormone metabolism in guinea pigs.in guinea pigs, activity of glutathione peroxidase in most organs is markedly lower than in organs of other rodents despite comparable dietary intakes and tissue levels of selenium. to determine if metabolism of selenium with respect to other selenoproteins also differs in guinea pigs, we measured the effects of selenium intake on thyroid hormone metabolism. weanling male hartley albino guinea pigs were fed a selenium-deficient torula yeast-based diet, or the same diet supplemented with 0.5 mg s ...19957861257
effects of brewer's yeast on glucose tolerance and serum lipids in chinese adults.this study was designed to investigate the supplemental effects of brewer's yeast on serum glucose and lipids in chinese adults. twenty-two participants (8 males and 14 females) were recruited from pingtung city. mean age of the group was 51 yr, and fasting values of total cholesterol and glucose were from 3.21 to 6.90 and 4.3 to 6.2 mmol/l, respectively. before supplementation, a 75 g oral glucose load was administered after an overnight (9 h) fast. blood was drawn before and at 30, 60, 90, and ...19947946924
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