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neuronal nitric oxide synthase and calbindin delineate sex differences in the developing hypothalamus and preoptic area.throughout the hypothalamus there are several regions known to contain sex differences in specific cellular, neurochemical, or cell grouping characteristics. the current study examined the potential origin of sex differences in calbindin expression in the preoptic area and hypothalamus as related to sources of nitric oxide. specific cell populations were defined by immunoreactive (ir) calbindin and neuronal nitric oxide synthase (nnos) in the preoptic area/anterior hypothalamus (poa/ah), anterov ...200717638388
prevalence of symptoms of asthma, rhinitis, and atopic eczema in brazilian adolescents related to exposure to gaseous air pollutants and socioeconomic status.to evaluate the relationship between exposure to gaseous air pollutants (ozone [o3], carbon monoxide [co], nitrogen dioxide [no2], and sulfur dioxide [so2]) socioeconomic status and the prevalence of symptoms of asthma, rhinitis and atopic eczema in adolescents.200717323857
multilocus phylogenetic analyses within blumeria graminis, a powdery mildew fungus of cereals.blumeria graminis, a powdery mildew fungus, is an important plant pathogen that causes serious damage to a variety of cereal crops. in spite of the importance of the pathogen, information on phylogenetic structure within b. graminis is scarce. in this study we conducted phylogenetic analyses of b. graminis based on the dna sequences of four different dna regions (its, 28s rdna, chitin synthase 1, and beta-tubulin). the analyses revealed that the protein-coding regions have higher amounts of phyl ...200717346991
[specific features of early embryogenesis in apomictic poa pratensis l].we studied the early stages of embryo formation in apomictic poa partensis l. it was shown that during transition to parthenogenesis, at least at the initial stages of embryogenesis, the algorithm of development of the sexual embryo is preserved. this could be due to the system of genetic control of embryogenesis, common for amphimixis and apomixis. we described asynchrony of developmental processes both within the efflorescence (asynchronous maturation of ovules) and within the ovule and even g ...200717352289
role of hypothalamus nociceptin/orphanin fq in pre-ovulatory luteinizing hormone surge of estrogen and progesterone-primed, ovariectomized rats.to investigate the role of hypothalamus nociceptin/orphanin fq (ofq) and its endogenous receptor, the opioid receptor-like1 receptor (orl1 receptor) in the estrus cycle of female rats.200717640482
the effect of stress on the expression of gnrh and gnrh receptor genes in the discrete regions of the hypothalamus and pituitary of anestrous ewes.using the reverse transcription-polymerase chain reaction (rt-pcr) technique, the gonadotropin releasing-hormone (gnrh) mrna and gnrh receptor (gnrh-r) mrna were analyzed in the preoptic area (poa), anterior (ah) and ventromedial (vm) hypothalamus, stalk/median eminence (sme) and anterior pituitary gland (ap) of anestrous ewes subjected to short or prolonged footshock stimulation. no gnrh gene expression was detected in the sme and ap. the comparable levels of gnrh mrna were found in the poa, ah ...200717435833
characteristic of hypothalamic kisspeptin expression in the pubertal development of precocious female rats.to investigate the potential role of kisspeptin in the advance onset of puberty in precocious puberty, model rats induced by danazol were used to study the developmental expression of hypothalamic kisspeptin. kisspeptin immunoreactive cells were observed in the arcuate nucleus (arc), periventricular nucleus (pen) and preoptic area (poa) in model rats on the day of onset-puberty. on the day of post-puberty, however, the number of kisspeptin immunoreactive cells in arc and pen decreased while the ...200717442487
reduced central c-fos expression and febrile response to repeated lps injection into periodontal tissue of rats.systemic injection of repeated doses of escherichia coli lipopolysaccharide (lps) results in attenuation of the febrile response, i.e. endotoxin tolerance, which has been fairly well characterized in rats. in the present study, we tested the hypothesis that endotoxin tolerance also occurs after repeated local injection of lps into periodontal protection tissue. male wistar rats were given a gingival intra-pouch injection of sterile saline or lps at dose of 100 microg/kg on three consecutive days ...200717445782
residual effects of imidacloprid on japanese beetle (coleoptera: scarabaeidae) oviposition, egg hatch, and larval viability in turfgrass.preventive control of turf-infesting scarabaeid grubs by neonicotinoid insecticides is presumed to mainly result from residues killing first instars in the soil. the extent to which sublethal behavioral effects or intoxication of other life stages contribute to such control is poorly known. we tested whether japanese beetle, popillia japonica newman, females lay fewer eggs in turf treated with imidacloprid (merit 75 wp) or an imidacloprid-bifenthrin combination (allectus gc sc), and whether expo ...200717461068
improvement in reproductive parameters in hypogonadal female mice by regulated gene replacement therapy in the central nervous system.one of the challenges of gene targeting is to achieve regulated transgene expression in specific target cells. the hypogonadal (hpg) mice are genetically deficient in hypothalamic gonadotropin-releasing hormone (gnrh) production due to a deletion in the gnrh gene, resulting in hypogonadotropic hypogonadism. here we show an improvement in reproductive parameters of adult female homozygous hpg mice by direct infusion into the hypothalamic preoptic area (poa) of a herpes simplex virus (hsv)-based a ...200717476303
bloodroot: life stories of nurse practitioners in rural appalachia.this nursing research study was designed to reveal the life stories of nurse practitioners in rural appalachia using storytelling and ballad elements of the culture. the researcher was born in a rural appalachian community in virginia and was summoned to return to the place of her birth, feeling an invisible kinship to land, blood ties to family, and a longing to honor rural nursing. seven nurse practitioners from the same community shared their life stories. using the heuristic phenomenological ...200717515560
use of wastewater and compost extracts as nutrient sources for growing nursery and turfgrass species.nutrient salts present in liquid by-products following waste treatment are lost resources if not effectively recycled, and can cause environmental problems if improperly disposed. this research compared the growth response and mineral nutrient status of two nursery and two turfgrass species, hydroponically supplied with nutritive by-product extracts derived from anaerobically digested municipal solid waste (msw) and aerobically composted organic wastes from the mushroom and msw industries. forsy ...200717526882
effects of season, testosterone and female exposure on c-fos expression in the preoptic area and amygdala of male green anoles.expression of the immediate early gene, c-fos, was used to investigate changes in neuronal activity in forebrain regions involved in male sexual behavior following social, hormonal and/or seasonal manipulations in the male green anole. these factors all influence behavior, yet it is unclear how they interact to modify neuronal activity in forebrain regions, including the preoptic area (poa) and ventromedial nucleus of the amygdala (amy). these regions are involved in the display of sexual behavi ...200717673187
preparing for poa reporting. how one facility educated its staff on the importance of the new requirement. 200717679605
layered confinement of protein in synthetic fluorinated mica via stepwise polyamine exchange.we have developed a process to incorporate the model protein, bovine serum albumin (bsa), into the layered spacing of swelled mica. by a stepwise intercalation, the sodium form of synthetic fluorinated mica (mica) was first exchanged with the poly(oxyalkylene)-diamine salts (poa-amine) through an ionic exchange reaction and then the bsa embedment. the first step of the mica space expansion from the pristine 12 a to 18-93 a was affected by hydrophobic pop-amines (pop2000 of 2000 g/mol and pop4000 ...200717685643
paradoxical ozone associations could be due to methyl nitrite from combustion of methyl ethers or esters in engine fuels.we review studies of the effects of low ambient ozone concentrations on morbidity that found a negative coefficient for ozone concentration. we call this a paradoxical ozone association (poa). all studies were in regions with methyl ether in gasoline. all but one study carefully controlled for the effects of other criterion pollutants, so the phenomenon cannot be attributed to them. one was in southern california in mid-summer when ozone levels are highest. because ozone is created by sunlight, ...200717716731
folding and misfolding of the collagen triple helix: markov analysis of molecular dynamics simulations.folding and misfolding of the collagen triple helix are studied through molecular dynamics simulations of two collagenlike peptides, [(pog)(10)](3) and [(pog)(4)poa(pog)(5)](3), which are models for wild-type and mutant collagen, respectively. to extract long time dynamics from short trajectories, we employ markov state models. by analyzing thermodynamic and kinetic quantities calculated from the markov state models, we examine folding mechanisms of the collagen triple helix and consequences of ...200717766343
estrogen modulates neuronal movements within the developing preoptic area-anterior hypothalamus.the preoptic area-anterior hypothalamus (poa-ah) is characterized by sexually dimorphic features in a number of vertebrates and is a key region of the forebrain for regulating physiological responses and sexual behaviours. using live-cell fluorescence video microscopy with organotypic brain slices, the current study examined sex differences in the movement characteristics of neurons expressing yellow fluorescent protein (yfp) driven by the thy-1 promoter. cells in slices from embryonic day 14 (e ...200717767488
distribution, abundance, and seasonal ecology of listronotus maculicollis (coleoptera: curculionidae) on golf courses in québec, canada.the weevil listronotus maculicollis dietz (coleoptera: curculionidae) is a major pest of annual bluegrass, poa annua l., on golf courses in northeastern north america. to determine the distribution, abundance, and seasonal ecology of l. maculicollis on golf courses in québec, canada, we sampled 19 golf courses (gc1-gc19) from different geographic and climatic conditions during 2001, 2002, and 2003. l. maculicollis was found on all golf courses except gc19, which was located in northeastern québe ...200717849888
effects of organisational oestradiol on adult immunoreactive oestrogen receptors (alpha and beta) in the male mouse brain.steroid hormones act on developing neural circuits that regulate the hypothalamic-pituitary-gonadal axis and are involved in hormone-sensitive behaviours. to test the hypothesis that developmental exposure to oestradiol (e(2)) organises the quantity of adult oestrogen receptors (eralpha and erbeta), we used male mice with a targeted mutation of the aromatase enzyme gene (arko) and their wild-type (wt) littermates. these mice are unable to aromatise testosterone to e(2), but still express both er ...200717850458
differences in ovarian function parameters between chinese and caucasian oocyte donors: do they offer an explanation for lower ivf pregnancy rates in chinese women?ivf outcomes in chinese women are inferior to those of caucasian patients. reflecting prematurely diminished ovarian function, women with elevated age-specific baseline (b-) fsh levels are designated to suffer from premature ovarian aging (poa). we investigated if the prevalence of poa differs between these two ethnic populations.200717855412
mango: a new approach to multiple sequence alignment.multiple sequence alignment is a classical and challenging task for biological sequence analysis. the problem is np-hard. the full dynamic programming takes too much time. the progressive alignment heuristics adopted by most state of the art multiple sequence alignment programs suffer from the 'once a gap, always a gap' phenomenon. is there a radically new way to do multiple sequence alignment? this paper introduces a novel and orthogonal multiple sequence alignment method, using multiple optimi ...200717951828
corticotropin releasing factor induces anxiogenic locomotion in trout and alters serotonergic and dopaminergic activity.corticotropin releasing factor (crf) and serotonin (5-ht) are strongly linked to stress and anxiety in vertebrates. as a neuromodulator in the brain, crf has anxiogenic properties often characterized by increased locomotion and stereotyped behavior in familiar environments. we hypothesized that expression of anxiogenic behavior in response to crf will also be exhibited in a teleost fish. rainbow trout were treated with intracerebroventricular (icv) injections of artificial cerebrospinal fluid (a ...200717826776
effects of temperature and light on the formation of chloroplast protrusions in leaf mesophyll cells of high alpine plants.chloroplasts of many alpine plants have the ability to form marked, stroma-filled protrusions that do not contain thylakoids. effects of temperature and light intensity on the frequency of chloroplasts with such protrusions in leaf mesophyll cells of nine different alpine plant species (carex curvula all., leontodon helveticus merat., oxyria digyna (l.) hill., poa alpina l. ssp. vivipara, polygonum viviparum l., ranunculus glacialis l., ranunculus alpestris l., silene acaulis l. and soldanella p ...200717897406
methoxyphenols and levoglucosan ratios in pm2.5 from wheat and kentucky bluegrass stubble burning in eastern washington and northern idaho.eastern washington is compromised by various pollution sources, of which agricultural burning is a particular burden. smoke from field burning is a nuisance to nearby communities and is a concern for health. this study evaluates levoglucosan (lg) and methoxyphenols (mps) as potential tracers for apportioning field burning smoke. pm2.5 (particulate matter < 2.5 microm m in aerodynamic diameter) samples from wheat and kentucky bluegrass (kbg) stubble smoke were collected from chamber and field bur ...200718075094
the final rule for fy 2008 ipps. ms-drgs and poa top list in a year of major change. 200718062443
central efferent pathways mediating skin cooling-evoked sympathetic thermogenesis in brown adipose tissue.control of thermoregulatory effectors by the autonomic nervous system is a critical component of rapid cold-defense responses, which are triggered by thermal information from the skin. however, the central autonomic mechanism driving thermoregulatory effector responses to skin thermal signals remains to be determined. here, we examined the involvement of several autonomic brain regions in sympathetic thermogenic responses in brown adipose tissue (bat) to skin cooling in urethane-chloralose-anest ...200716931649
assessing exclusionary power of a paternity test involving a pair of alleged grandparents.the power of a genetic test battery to exclude a pair of individuals as grandparents is an important consideration for parentage testing laboratories. however, a reliable method to calculate such a statistic with short-tandem repeat (str) genetic markers has not been presented.200717302781
exploration of prostanoid receptor subtype regulating estradiol and prostaglandin e2 induction of spinophilin in developing preoptic area neurons.the prostaglandin e2 (pge2) mediates estradiol-induced masculinization of sexual behavior in the rat during a perinatal sensitive period. pge2 induces formation of dendritic spines on preoptic area (poa) neurons and this synaptic pattern change is associated with the ability to express male sexual behavior as an adult. whether pge2 is released from astrocytes or neurons in the developing poa is unknown. to further understanding of how pge2 induces dendritic spine formation at the cellular level, ...200717408863
control of gastrointestinal nematodes with copper oxide wire particles in a flock of lactating polypay ewes and offspring in iowa, usa.copper oxide wire particles (cowp) have been used to reduce infection of haemonchus contortus in hair breed lambs in southeastern usa without signs of copper toxicity. however, copper sensitivity among breeds and regions varies. the objective was to determine the effectiveness and safety of cowp in lactating polypay ewes and their offspring grazing alfalfa/bluegrass pasture in a rotational grazing system. mature polypay ewes were administered 0, 0.5, 1, or 2 g (n=8 or 9/dose) cowp approximately ...200717433548
ccr1 and ccr5 chemokine receptors are involved in fever induced by lps (e. coli) and rantes in rats.this study, besides examining the involvement of ccr1 and ccr5 receptors in the lps-induced fever (lipopolysaccharide, escherichia coli) in male wistar rats, evaluated if rantes (regulated on activation, normal t cells expressed and secreted) injected into the preoptic area of the anterior hypothalamus (ah/poa) would promote an integrated febrile response via these receptors. moreover, the effects of selective and non-selective cyclooxygenase blockers on both fever and the level of prostaglandin ...200717604006
an exploration of the utility of hypnosis in pain management among rural pain patients.hypnosis is an adjunctive, noninvasive treatment with few side effects that can be useful in the management of chronic pain. however, it has fallen into disfavor in recent years and is often perceived by physicians as simple charlatanism. we evaluated the efficacy of this treatment as used clinically in a large, mostly rural, pain management center.200717578065
preliminary investigation of changes in the sexually dimorphic nucleus of the rat medial preoptic area following prenatal exposure to fenitrothion.in vitro, the organophosphate insecticide fenitrothion is a potent competitive androgen receptor antagonist, whereas in vivo it affects the development of the male rat reproductive system. the purpose of this pilot study was to determine whether prenatal exposure to fenitrothion affects development of the rat sexually dimorphic nucleus of the medial preoptic area (sdn-poa). pregnant rats (n = 5-6 litters/group) were orally dosed with corn oil (vehicle) or fenitrothion (20 or 25 mg kg(-1) day(-1) ...200717582585
androgen receptors in a cichlid fish, astatotilapia burtoni: structure, localization, and expression levels.androgens are an important output of the hypothalamic-pituitary-gonadal (hpg) axis that controls reproduction in all vertebrates. in male teleosts two androgens, testosterone and 11-ketotestosterone, control sexual differentiation and development in juveniles and reproductive behavior in adults. androgenic signals provide feedback at many levels of the hpg axis, including the hypothalamic neurons that synthesize and release gonadotropin-releasing hormone 1 (gnrh1), but the precise cellular site ...200717614300
prairie and turf buffer strips for controlling runoff from paved surfaces.eutrophication of surface waters due to nonpoint source pollution from urban environments has raised awareness of the need to decrease runoff from roads and other impervious surfaces. these concerns have led to precautionary p application restrictions on turf and requirements for vegetative buffer strips. the impacts of two plant communities and three impervious/pervious surface ratios were assessed on runoff water quality and quantity. a mixed forb/grass prairie and a kentucky bluegrass (poa pr ...200717255630
tryptophan-induced central fatigue in exercising rats is related to serotonin content in preoptic area.to assess the effects of increased hypothalamic tryptophan (trp) availability on 5-ht content in preoptic area on thermoregulation and work production during exercise on treadmill, 20.3 microm of l-trp (n=7) or 0.15m nacl (n=6) was injected into the lateral cerebral ventricle of male wistar rats immediately before the animals started running (18 m min(-1) 5% inclination). exercise time to fatigue (min), and workload (kgm) were analysed. core temperature was measured by telemetry. at fatigue, bra ...200717280786
the effects of gaba on embryonic gonadotropin-releasing hormone neurons in rat hypothalamic primary culture.gonadotropin-releasing hormone (gnrh) neurons arise in the olfactory placode, migrate into the preoptic area (poa), and then extend axons to the median eminence during embryogenesis. little information is available concerning the properties of gnrh neurons during the late gestational period when gnrh neurons reach the poa and form neuronal networks, although many studies have examined such properties during earlier developmental stages or the postnatal period. the present study was performed to ...200717179652
regulation of summer dormancy by water deficit and aba in poa bulbosa ecotypes.survival of many herbaceous species in mediterranean habitats during the dry, hot summer depends on the induction of summer dormancy by changes in environmental conditions during the transition between the winter (growth) season to the summer (resting) season, i.e. longer days, increasing temperature and drought. in poa bulbosa, a perennial geophytic grass, summer dormancy is induced by long days, and the induction is enhanced by high temperature. here the induction of summer dormancy in a medit ...200717202183
are you ready for poa reporting? 200717285905
effects of castration and hormone replacement on male sexual behavior and pattern of expression in the brain of sex-steroid receptors in balb/c ann mice.little is known about the hormonal regulation of sexual behavior and about the pattern of expression in the brain of sex-steroid receptors in the balb/c ann strain of mice (mus musculus). in this study, 8-week old male balb/c ann mice were castrated and the temporal course of decline of sexual behavior was studied, as well as the effects of daily treatment with either testosterone propionate (tp), estradiol benzoate (eb), or dihydrotestosterone propionate (pdht). castration resulted in rapid dec ...200717292648
identification of two novel phytosiderophores secreted by perennial grasses.it has been suggested that some perennial grasses secrete phytosiderophores in response to iron (fe) deficiency, but the compounds have not been identified. here, we identified and characterized the phytosiderophores secreted by two perennial grasses, lolium perenne cv. tove and poa pratensis cv. baron. root exudates were collected from the roots of fe-deficient grasses and then purified with various chromatographies. the structure of the purified compounds was determined using both nuclear magn ...200717388893
changing leaf litter feedbacks on plant production across contrasting sub-arctic peatland species and growth forms.plant species and growth forms differ widely in litter chemistry, which affects decay and may have important consequences for plant growth via e.g. the release of nutrients and growth-inhibitory compounds. we investigated the overall short-term (9.5 months) and medium-term (21.5 months) feedback effects of leaf litter quality and quantity on plant production, and tested whether growth forms can be used to generalise differences among litter species. leaf litter effects of 21 sub-arctic vascular ...200717089140
bilateral damage to the sexually dimorphic medial preoptic area/anterior hypothalamus of male ferrets causes a female-typical preference for and a hypothalamic fos response to male body odors.previous studies showed that bilateral lesions of the male ferret's preoptic area/anterior hypothalamus (poa/ah), centered in the sexually dimorphic nuclei present in this region, caused subjects to seek out a same-sex male, as opposed to a female conspecific. male subjects with poa/ah lesions (which were also castrated and given estradiol) displayed female-typical receptive behavior in response to neck gripping by a stimulus male, implying that subjects' approaches to a same-sex conspecific wer ...200717118411
characterization of rainbow trout egg quality: a case study using four different breeding protocols, with emphasis on the incidence of embryonic malformations.the aim of this study was to set up a methodology to accurately evaluate the effects of various husbandry practices on a fish broodstock based on the developmental potential of the egg. for that purpose, long-short photoperiod manipulations (tested twice, pm1 and pm2 groups), spawning induction by injection of a gnrh analog (si group), and a 16-day post-ovulatory ageing of eggs (poa group) were used in rainbow trout (oncorhynchus mykiss). females without any treatment were used as a control grou ...200717118435
environmental effects on grass-endophyte associations in the harsh conditions of south patagonia.cool-season grasses are frequently infected by neotyphodium endophytes and this association is often considered as a mutualistic symbiosis. we examined the incidence of neotyphodium in populations of bromus setifolius, phleum alpinum and poa spiciformis, native and wide-spread grasses from south patagonia, argentina. the incidence of 36 populations of bromus setifolius was studied in association with climatic and soil variables. 31 populations of ph. alpinum were sampled in five different plant ...200717466027
transient transcription of the somatostatin gene at the time of estrogen-dependent organization of the sexually dimorphic nucleus of the rat preoptic area.in situ hybridization detected a transient, sex-specific transcription of somatostatin gene in the central part of the rat medial preoptic nucleus, which coincides with the sexually dimorphic nucleus of the preoptic area (sdn-poa), during, but not after, the establishment of sex difference. on postnatal d 1 (day of birth), somatostatin mrna was detected in the sdn-poa of both sexes. on d 8 through 35, the area of somatostatin mrna-positive cells was significantly larger in males than in females. ...200717138650
shifts in positive and negative plant interactions along a grazing intensity gradient.isolating the single effects and net balance of negative and positive species effects in complex interaction networks is a necessary step for understanding community dynamics. facilitation and competition have both been found to operate in harsh environments, but their relative strength may be predicted to change along gradients of herbivory. moreover, facilitation effects through habitat amelioration and protection from herbivory may act together determining the outcome of neighborhood plant-pl ...200717489467
modifying drg-pps to include only diagnoses present on admission: financial implications and challenges.the inability to distinguish complications acquired in hospital from comorbid conditions that are present on admission (poa) has long hampered the use of claims data in quality and safety research. now pay-for-performance initiatives and legislation requiring medicare to reduce payment for acquired infections add imperative for poa coding. this study used data from 2 states currently coding poa to assess the financial impact if medicare pays based on poa conditions only and to examine the challe ...200717496711
evaluation of the potential role of water in spread of conidia of the neotyphodium endophyte of poa ampla.neotyphodium endophytes are asexual, filamentous fungi, mutualistically associated with diverse cool season grasses. infected seeds and vegetative organs of infected host plants are the only known modes of propagation of the asexual endophytes. in the last decade certain epichloë and neotyphodium-infected grass species have been shown to have epiphyllous structures of the endophytes, hyphae, conidiophores, and conidia, growing on leaf blades. the production of epiphyllous conidia suggests the po ...200717512714
[hip paraosteoarthropathy after sciatica nerve injury by quinine intramuscular injection].often occurring in central nerve injuries, paraosteoarthropathic conditions are revealed by a stiffness or ankylosis of the joint. their occurring during peripheral nerve injuries is rare.200717141911
courtship and copulation in the adult male green anole: effects of season, hormone and female contact on reproductive behavior and morphology.interactions among reproductive season, testosterone (t) and female presence were investigated on the structure and function of forebrain and neuromuscular systems controlling courtship and copulation in the green anole lizard. under breeding (bs) or non-breeding (nbs) environmental conditions, male green anoles were implanted with either t or blank capsules and exposed to one of three female stimulus conditions: physical, visual or no female contact. t and at least visual exposure to females in ...200717174414
the effects of long-term grazing exclosures on range plants in the central anatolian region of turkey.over the last fifty years, almost half of the steppe rangeland in the central anatolian region of turkey (car) has been converted to cropland without an equivalent reduction in grazing animals. this shift has led to heavy grazing pressure on rangeland vegetation. a study was initiated in june 2003 using 6 multiscale modified-whittaker plots to determine differences in plant composition between areas that have not been grazed in 27 years with neighboring grazed plant communities. a total of 113 p ...200717203339
involvement of anteroventral periventricular metastin/kisspeptin neurons in estrogen positive feedback action on luteinizing hormone release in female rats.metastin/kisspeptin, the kiss-1 gene product, has been identified as an endogenous ligand of gpr54 that reportedly regulates gnrh/lh surges and estrous cyclicity in female rats. the aim of the present study was to determine if metastin/kisspeptin neurons are a target of estrogen positive feedback to induce gnrh/lh surges. we demonstrated that preoptic area (poa) infusion of the anti-rat metastin/kisspeptin monoclonal antibody blocked the estrogen-induced lh surge, indicating that endogenous meta ...200717213691
ventromedial hypothalamic nucleus lesions disrupt olfactory mate recognition and receptivity in female ferrets.previous research showed that ferrets of both sexes rely on the perception of conspecifics' body odors to identify and motivate approach towards opposite-sex mating partners, and exposure to male body odors stimulated fos expression in an olfactory projection circuit of female, but not male, ferrets that terminates in the ventromedial hypothalamic nucleus (vmh). we asked whether the female-typical preference of ferrets to approach male as opposed to female body odors in y-maze tests would be dis ...200717011561
characterization and in vitro degradation of poly(octadecanoic anhydride).poly(octadecanoic anhydride) (poa) has been prepared by melt polycondensation of octadecanoic diacid. poa was characterized by fourier-transform infrared spectroscopy (ftir), differential scanning calorimetry (dsc) and wide angle x-ray diffraction (waxd). the results of in vitro degradation and sem micrographs show that the erosion process of poa is neither bulk nor perfect surface erosion but rather has elements of both in phosphate buffer at 37 degrees c. the moving erosion front is characteri ...200817577635
ovarian steroids stimulate adenosine triphosphate-sensitive potassium (katp) channel subunit gene expression and confer responsiveness of the gonadotropin-releasing hormone pulse generator to katp channel modulation.the atp-sensitive potassium (k(atp)) channels couple intracellular metabolism to membrane potential. they are composed of kir6.x and sulfonylurea receptor (sur) subunits and are expressed in hypothalamic neurons that project to gnrh neurons. however, their roles in regulating gnrh secretion have not been determined. the present study first tested whether k(atp) channels regulate pulsatile gnrh secretion, as indirectly reflected by pulsatile lh secretion. ovariectomized rats received sc capsules ...200818258681
seabream gnrh immunoreactivity in brain and pituitary of xx and xy nile tilapia, oreochromis niloticus during early development.seabream gonadotropin-releasing hormone (sbgnrh)-the chief preoptic area-hypothalamus (poa-h) form of gnrh in tilapia is involved in sexual maturation. in this study, we investigated the qualitative changes in ontogeny of sbgnrh immunoreactivity (ir-), between sexes to understand its impending role during sex differentiation. for this, the differences in immunocytochemical localization of sbgnrh in genetically male (xy) and female (xx) fish were studied from 1 day after hatching (dah), through t ...200818521911
does autoimmunity play a role in the pathophysiology of premature ovarian ageing?to determine a possible causative association between autoimmunity and premature ovarian ageing (poa), 394 women undergoing ivf at an academically affiliated private ivf centre were retrospectively evaluated for the presence of autoimmune-related diseases. out of 162 poa women, 13 (8.0%) demonstrated a history of autoimmune disease(s), while autoimmunity was present in 28 (12.1%) of 232 controls. poa women with poor response (four oocytes or fewer) despite high-dose treatment presented autoimmun ...200818549693
coraco- or costoclavicular paraosteoarthropathies in patients with severe central neurological disorders.paraosteoarthropathy (poa) is a frequent disabling orthopedic complication after severe central neurological impairment. the hip is the most frequently affected joint (32.1%) followed by the elbow and the shoulder (25%).200818300141
heads or tails? stressor-specific expression of corticotropin-releasing factor and urotensin i in the preoptic area and caudal neurosecretory system of rainbow trout.corticotropin-releasing factor (crf)- and urotensin i (ui)-expressing cells of the preoptic area (poa) and caudal neurosecretory system (cnss) are considered key contributors to the regulation of the stress response in fish; however, the expression pattern of these neurons to environmental and social challenges have not been compared in a single study. therefore, we characterized in rainbow trout (oncorhynchus mykiss) the central distribution of crf and ui expression and quantified the poa and c ...200818310459
winter photosynthetic activity of twenty temperate semi-desert sand grassland species.the winter photosynthetic activity (quantified by net co(2) assimilation rates and chlorophyll (chl) a fluorescence parameters) of 20 plant species (including two lichens and two mosses) of a hungarian temperate semi-desert sand grassland was determined on one occasion per year in 1984, 1989 and 1994. throughout winter, the overwintering green shoots, leaves or thalli were regularly exposed to below zero temperatures at night and daytime temperatures of 0-5 degrees c. in situ tissue temperature ...200818346813
medicare's decision to withhold payment for hospital errors: the devil is in the det.background: medicare recently announced its intention to withhold additional payments for "serious preventable events." the intervention: beginning in 2009, medicare will withhold its usual additional payments associated with hospitalizations that included one of several potentially preventable adverse events, such as certain hospital-acquired infections, pressure ulcers, and retained surgical objects. several more events are being considered for the future. a new coding category, "present on ad ...200818351196
[seed dispersal efficiency of forest herbaceous plant species by the ant myrmica ruginodis].we compared the ability of 33 plant species collected in forest to have their seeds dispersed by the wood red ant myrmica ruginodis. the elaiosome-bearing seeds (species chelidonium majus, colchicum autumnale, luzula forsteri, viola reichenbachiana, luzula multiflora, melica uniflora) and the small and light caryopses of the grasses (holcus mollis, agrostis capillaris, deschampsia flexuosa, poa trivialis, holcus lanatus) are the most efficiently collected by ants. the seed quantity removed by an ...200818355754
site-specific regulation of gene expression by estrogen in the hypothalamus of adult female rats.estrogen plays critical roles in the neuroendocrine system of adult female rats through separate actions, respectively, in the preoptic area (poa) and the ventromedial nucleus of the hypothalamus (vmh). seven-week-old rats were treated with/without estrogen after they were ovariectomized, and four estrogen-responsive, neuronal system-related genes, encoding alpha4 neuronal nicotinic acetylcholine receptor (chrna4), gaba(a) receptor delta (gabrd), serotonin receptor 6 (htr6), and gaba transporter ...200818358606
specific immunotherapy for common grass pollen allergies: pertinence of a five grass pollen vaccine.patients throughout europe are concomitantly exposed to multiple pollens from distinct pooideae species. given the overlap in pollination calendars and similar grain morphology, it is not possible to identify which grass species are present in the environment from pollen counts. furthermore, neither serum ige reactivity nor skin prick testing allow the identification of which grass species are involved in patient sensitisation. due to their high level of amino acid sequence homology (e.g., >90% ...200818362477
analysis of poa and hospital-acquired conditions data. part 1. 200818604981
o/c and om/oc ratios of primary, secondary, and ambient organic aerosols with high-resolution time-of-flight aerosol mass spectrometry.a recently developed method to rapidly quantify the elemental composition of bulk organic aerosols (oa) using a high-resolution time-of-flight aerosol mass spectrometer (hr-tof-ams) is improved and applied to ambient measurements. atomic oxygen-to-carbon (o/c) ratios characterize the oxidation state of oa, and o/c from ambient urban oa ranges from 0.2 to 0.8 with a diurnal cycle that decreases with primary emissions and increases because of photochemical processing and secondary oa (soa) product ...200818605574
the preoptic anterior hypothalamic area mediates initiation of the hypotensive response induced by lps in male rats.the mechanism responsible for the initiation of endotoxic hypotension is not fully understood, although it is often attributed to a direct effect of lps and other vasoactive mediators on the vasculature. alternatively, recent evidence raises the possibility that endotoxic hypotension may be initiated through a central mechanism. previous studies have shown that lps initiates fever, sickness behavior, and other aspects of the inflammatory response through a neural pathway that sends peripheral in ...200818386391
preoptic mechanism for cold-defensive responses to skin cooling.we recently identified a somatosensory pathway that transmits temperature information from the skin to a median subregion of the preoptic area (poa), a thermoregulatory centre. here, we investigated in vivo the local neuronal circuit in the rat poa that processes the thermosensory information and outputs thermoregulatory effector signals. skin cooling-evoked increases in sympathetic thermogenesis in brown adipose tissue, in metabolism and in heart rate were reversed by inhibition of neurons in t ...200818388139
enzyme kinetics and binding studies on inhibitors of mek protein kinase.inhibition of the protein kinase, mek1, is a potential approach for the treatment of cancer. inhibitors may act by prevention of activation (poa), which involves interfering with phosphorylation of nonactivated mek1 by the upstream kinase, b-raf. modulation also may occur by inhibition of catalysis (ioc) during phosphorylation of the downstream substrate, erk2, by activated mek1. here, five mek inhibitors are characterized in terms of binding affinity, poa, and ioc. the compounds are a butadiene ...200818393446
mango: multiple alignment with n gapped oligos.multiple sequence alignment is a classical and challenging task. the problem is np-hard. the full dynamic programming takes too much time. the progressive alignment heuristics adopted by most state-of-the-art works suffer from the "once a gap, always a gap" phenomenon. is there a radically new way to do multiple sequence alignment? in this paper, we introduce a novel and orthogonal multiple sequence alignment method, using both multiple optimized spaced seeds and new algorithms to handle these s ...200818574861
courtship interactions stimulate rapid changes in gnrh synthesis in male ring doves.many birds and mammals show changes in the hypothalamo-pituitary-gonadal (hpg) axis in response to social or sexual interactions between breeding partners. while alterations in gnrh neuronal activity play an important role in stimulating these changes, it remains unclear if acute behaviorally-induced alterations in gnrh release are accompanied by parallel changes in gnrh synthesis. to investigate this relationship, we examined changes in the activity of gnrh neurons in the brains of male ring do ...200818706906
poa coding challenges. concern grows over quality of documentation. 200818711904
sex and seasonal differences in morphology of limbic forebrain nuclei in the green anole lizard.sex and seasonal differences in the brain occur in many species and are often related to behavioral expression. for example, morphology of limbic regions involved in male sex behavior are larger in males than in females, and sometimes are larger in the breeding than non-breeding season. morphology can often be altered in adulthood by manipulating levels of steroid hormones. in untreated green anole lizards, previous work indicated that neuron soma size and density did not differ between the sexe ...200818598684
nonclassical mechanisms of progesterone action in the brain: ii. role of calmodulin-dependent protein kinase ii in progesterone-mediated signaling in the hypothalamus of female rats.in addition to the activation of classical progestin receptor-dependent genomic pathway, progesterone (p) can activate nonclassical, membrane-initiated signaling pathways in the brain. we recently demonstrated rapid p activation of second-messenger kinases, protein kinase a, and protein kinase c in the ventromedial nucleus (vmn) and preoptic area (poa) of rat brain. to determine whether p can activate yet another ca+2 dependent kinase, we examined the rapid p modulation of calcium and calmodulin ...200818617607
nonclassical mechanisms of progesterone action in the brain: i. protein kinase c activation in the hypothalamus of female rats.the modulation of gene regulation by progesterone (p) and its classical intracellular regulation by progestin receptors in the brain, resulting in alterations in physiology and behavior has been well studied. the mechanisms mediating the short latency effects of p are less well understood. recent studies have revealed rapid nonclassical signaling action of p involving the activation of intracellular signaling pathways. we explored the involvement of protein kinase c (pkc) in p-induced rapid sign ...200818617608
transcatheter occlusion of antegrade pulmonary flow in children after cavopulmonary anastomosis.to report our experience with transcatheter occlusion of antegrade pulmonary blood flow (apf) for postoperative complications of cavopulmonary anastomosis (bcpa).200819021287
ecdysteroid ligand-receptor selectivity--exploring trends to design orthogonal gene switches.a set of thirty-two natural and ten semisynthetic ecdysteroids was assayed in murine 3t3 cells across ten different ecdysteroid receptor (ecr) ligand-binding domains derived from nine arthropod species in an engineered gene switch format. among the ecdysteroids tested, the most biologically widespread ecdysteroid, 20-hydroxyecdysone (20e), was moderately and consistently potent across the tested ecrs. the most potent ligand-receptor combination (ec(50) = 0.3 nm) was ponasterone a (poa) actuating ...200819021756
lipopolysaccharide-induced hypotension is mediated by a neural pathway involving the vagus nerve, the nucleus tractus solitarius and alpha-adrenergic receptors in the preoptic anterior hypothalamic area.we recently reported that the preoptic anterior hypothalamic area (poa) mediates the hypotensive response evoked by lipopolysaccharide (lps). in this study, we investigated how the inflammatory signal induced by lps reaches the poa. subdiaphragmatic vagotomy and abdominal perivagal lidocaine administration, or lidocaine injection into the nucleus tractus solitarius (nts) prevented lps hypotension. microinjection of the alpha-adrenergic receptor antagonist phentolamine into the poa, blocked initi ...200818653249
blockade of prostaglandin e2-induced thermogenesis by unilateral microinjection of gabaa receptor antagonist into the preoptic area.previous studies have demonstrated that pretreatment of rats with a gaba(a) receptor antagonist microinjected bilaterally into the preoptic area (poa) blocked cold- or lipopolysaccharide-induced thermogenesis. here, the involvement of gaba(a) receptors in prostaglandin (pg)e2-induced fever was examined. thermogenic, tachycardic, vasoconstrictive, and hyperthermic responses were elicited by the unilateral microinjection of 0.57-1.1 pmol pge2 into the region adjacent to the organum vasculosum of t ...200818662676
analysis of poa and hospital-acquired conditions data. part 2. 200818672732
association of cocaine- and amphetamine-regulated transcript and neuropeptide y in the forebrain and pituitary of the catfish, clarias batrachus: a double immunofluorescent labeling study.cocaine- and amphetamine-regulated transcript (cart) and neuropeptide y (npy) are involved in the regulation of food intake, body weight, pituitary hormones, and reproduction. while cart and npy occupy overlapping fields in the brain of mammals, little is known about the interaction between these peptide-containing systems in other vertebrates. we explored neuroanatomical associations between cart and npy in the olfactory system, forebrain and pituitary of the catfish, clarias batrachus, using d ...200818675898
cytokine-induced neutrophil chemoattractant (cinc)-1 induces fever by a prostaglandin-dependent mechanism in rats.cytokine-induced neutrophil chemoattractant-1 (cinc-1), a member of the elr+cxc subfamily [elr motif (glutamic acid-leucine-arginine) adjacent to the cysteine-x-cysteine (cxc) motif located at the n-terminus of the protein], is an acute-phase protein and its synthesis is induced by endogenous and exogenous pyrogens. however, there are no studies on the pyrogenic property of cinc-1. therefore, the present study evaluates whether centrally administered cinc-1 promotes an integrated febrile respons ...200818694739
impact of date stamping on patient safety measurement in patients undergoing cabg: experience with the ahrq patient safety indicators.the agency for healthcare research and quality (ahrq) patient safety indicators (psis) provide information on hospital risk-adjusted rates for potentially preventable adverse events. although designed to work with routine administrative data, it is unknown whether the psis can accurately distinguish between complications and pre-existing conditions. the objective of this study is to examine whether the ahrq psis accurately measure hospital complication rates, using the data with present-on-admis ...200818700979
combining winter hardiness and forage yield in white clover (trifolium repens) cultivated in northern environments.white clover (trifolium repens) is an important component of sustainable livestock systems around the world. its exploitation for agriculture in the northern, marginal areas is, however, currently limited by the lack of cultivars that combine persistence and high production potential. the aims are to investigate whether it is feasible to create breeding material of white clover for these areas by combining winter hardiness of northerly populations with good yielding ability of more southerly cul ...200818772149
breaking the code. poa documentation policy confusing for some. 200818780428
identification of prostaglandin e2 receptors mediating perinatal masculinization of adult sex behavior and neuroanatomical correlates.prostaglandin e2 (pge2) mediates the organization of male rat sexual behavior and medial preoptic area (mpoa) neuroanatomy during a sensitive perinatal window. pge2 is up-regulated in response to estradiol, and initiates a two-fold increase in dendritic spines densities on neurons. all the four receptors for pge2 and ep1-4 are present in developing poa, a critical region controlling male sexual behavior. previous studies explored that ep receptors are involved in pge2-induction of neonatal level ...200818726914
mesophyll freezing and effects of freeze dehydration visualized by simultaneous measurement of idta and differential imaging chlorophyll fluorescence.infrared differential thermal analysis (idta) and differential imaging chlorophyll fluorescence (dif) were employed simultaneously to study the two-dimensional pattern of ice propagation in leaves and mesophyll freeze dehydration as detected by a significant increase of basic chlorophyll fluorescence (f(0)). idta and dif technique gave different insights into the freezing process of leaves that was highly species-specific. idta clearly visualized the freezing process consisting of an initial fas ...200818761699
automated respiratory inductive plethysmography to evaluate breathing in infants at risk for postoperative apnea.although respiratory inductive plethysmography (rip) is the method of choice for the assessment of sleep disordered breathing, it has not been applied to the study of infants at risk for postoperative apnea (poa). the purpose of this study was to apply rip to evaluate breathing in these infants. an additional purpose was to implement, simultaneously, three novel algorithms to detect movement artifact, respiratory pauses, and thoracoabdominal asynchrony, since their combined output both detects r ...200819138913
patterns of variation in the seasonal dynamics of listronotus maculicollis (coleoptera: curculionidae) populations on golf course turf.listronotus maculicollis (kirby) is a burgeoning pest of poa annua l. in the northeast united states. to develop an understanding of its spatial and temporal association with golf course landscapes, we described the patterns of variation in certain population parameters across site (fayetteville and ithaca, ny), year (2004-2006), and management habitat (fairway, intermediaterough, rough). in weekly surveys along fairway transects, we sampled larvae (identified to instar) by soil core extraction ...200819161687
[effect of exogenous androgen on structures of sexually dimorphism nucleus in preoptic area and anteroventral periventricular nucleus before sexual differentiation in female rats].to investigate the effects of androgen on sexually dimorphism nucleus in preoptic area (sdn-poa) and anteroventral periventricular nucleus (avpv) before sexual differentiation of the brain in female rats.200818925716
transgenerational effects of global environmental change: long-term co(2) and nitrogen treatments influence offspring growth response to elevated co(2).global environmental changes can have immediate impacts on plant growth, physiology, and phenology. long-term effects that are only observable after one or more generations are also likely to occur. these transgenerational effects can result either from maternal environmental effects or from evolutionary responses to novel selection pressures and are important because they may alter the ultimate ecological impact of the environmental change. here, we show that transgenerational effects of atmosp ...200818716799
neurosecretory neurons of the nucleus preopticus (npo) express salmon gnrh mrna and show reproduction phase-related variation in the female indian major carp, cirrhinus cirrhosus.we studied the expression of sgnrh mrna in the neurons of the nucleus preopticus (npo) of the indian major carp, cirrhinus cirrhosus, and their correlation with the reproductive status of the fish. non-radioisotopic in situ hybridization histochemistry protocol employing biotinylated-oligonucleotide probes complementary to salmon gnrh, cichlid gnrh i, catfish gnrh, chicken gnrh ii (from cichlid and catfish), and mammalian gnrh, were applied to the sections through the poa of the female indian ma ...200818664387
effects of surgical induction of endometriosis on response properties of preoptic area neurons in rats.subfertility and severe pelvic pains are symptoms associated with endometriosis (endo), a common condition among women that is characterized by the growth of the uterine endometrium on the surface of organs within the pelvic region and abdominal cavity. the contribution of the cns to symptoms associated with endo is not known. in the present study, the preoptic area (poa) of the hypothalamus was investigated, as this region of the forebrain is known to play an important role in the neuroendocrin ...200818955036
investigation of inflammatory and hemostatic parameters in female patients undergoing total knee arthroplasty surgery.tendency to hypercoagulation is a common phenomenon in primary osteoarthritis patients (poa) undergoing total knee arthroplasty (tka) surgery, but the clinical implications of this condition are not clear. therefore we aimed to evaluate the inflammatory and coagulation parameters in the patient group and find a possible explanation for the tendency to hypercoagulation occurring in plasma and synovia of inflamed joints. of the evaluated factors involved in inflammation and coagulation, galectin-3 ...200819009338
spatial and temporal patterns of carabid activity-density in cereals do not explain levels of predation on weed seeds.seed predation is an important component of seed mortality of weeds in agro-ecosystems, but the agronomic use and management of this natural weed suppression is hampered by a lack of insight in the underlying ecological processes. in this paper, we investigate whether and how spatial and temporal variation in activity-density of granivorous ground beetles (coleoptera: carabidae) results in a corresponding pattern of seed predation. activity-density of carabids was measured by using pitfall traps ...200818076785
alpha-oestrogen and progestin receptor expression in the hypothalamus and preoptic area dopaminergic neurones during oestrous in cycling rats.a secretory surge of prolactin occurs on the afternoon of oestrous in cycling rats. although prolactin is regulated by ovarian steroids, plasma oestradiol and progesterone levels do not vary during oestrous. because prolactin release is tonically inhibited by hypothalamic dopamine and modulated by dopamine transmission in the preoptic area (poa), the present study aimed to evaluate whether oestrogen receptor (er)-alpha and progestin receptor (pr) expression in the dopaminergic neurones of arcuat ...200818081559
convergence of nociceptive information in the forebrain of female rats: reproductive organ response variations with stage of estrus.neurons in the preoptic area (poa) of the hypothalamus and the bed nucleus of stria terminalis (bst) play an important role in the neuroendocrine control of the reproductive cycle, mating behaviors and nociception. single unit extracellular recordings were performed in the poa and bst region of 20 urethane anesthetized female rats during either the proestrus (elevated levels of estrogen/progesterone) or metestrus (low circulating hormones) stage of the estrous cycle. a total of 118 neurons in th ...200818096159
racs, poa, and the unstoppable evolution of healthcare. 200818162963
estrogen modulates bcl-2 family protein expression in the sexually dimorphic nucleus of the preoptic area of postnatal rats.in the sexually dimorphic nucleus of the preoptic area (sdn-poa) of postnatal rats, apoptotic cells are detected more frequently in females than males. this sex difference is under the influence of aromatized androgen. we have reported that there are sex differences in the levels of bcl-2 (female<male) and bax (female>male) in the central division of the medial preoptic nucleus (mpnc), a significant component of the sdn-poa, followed by a sex difference in induction of apoptosis via caspase-3 ac ...200818164816
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