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[anti-erosion effect of hedgerows in hillside croplands of danjiangkou based on the evaluation with water erosion prediction project (wepp) model].based on the data of field experiments on the hillside croplands of danjiangkou, hubei province of china, the input files of crop characters, management measures, slope gradient and length, and soil properties for running wepp model (hillslope version) were established. combining with the local weather data, a simulation study with the model was made on the runoff and soil loss of the croplands protected by four kinds of hedgerows (amorpha fruticosa, lonicera japonica, hemerocallis fulva, and po ...201021265164
androgens alter brain catecholamine content and blood pressure in the testicular feminized male rat.androgens interact with catecholamines in the central nervous system (cns) to regulate many physiological processes including blood pressure (bp). to test the hypothesis that testosterone (t) and 5a-dihydrotestosterone (dht) modulate cns catecholamines and bp through androgen receptor (ar)-dependent and independent mechanisms, we used the testicular feminized male (tfm) rat. females that carry the ar mutation (tfm mutation) on the x chromosome were bred with spontaneously hypertensive rat (shr) ...201121271819
hypoxia-induced hypothermia mediated by noradrenaline and nitric oxide in the rostromedial preoptic area.central nitric oxide (no) has an important role in hypothermia induced by hypoxia as well as in that elicited by noradrenaline (na) microinjected into the rostromedial preoptic area (poa) of the hypothalamus. here, i tested the hypothesis that activation of adrenoceptors and no in the rostromedial poa is involved in hypoxia-induced hypothermia in urethane-chloralose-anesthetized, neuromuscularly blocked, artificially ventilated rats. hypoxic ventilation (10% o2-90% n2, 5 min) evoked an increase ...201121277355
prenatal pcbs disrupt early neuroendocrine development of the rat hypothalamus.neonatal exposure to endocrine disrupting chemicals (edcs) such as polychlorinated biphenyls (pcbs) can interfere with hormone-sensitive developmental processes, including brain sexual differentiation. we hypothesized that disruption of these processes by gestational pcb exposure would be detectable as early as the day after birth (postnatal day (p) 1) through alterations in hypothalamic gene and protein expression. pregnant sprague-dawley rats were injected twice, once each on gestational days ...201121277884
investigation on injectable, thermally and physically gelable poly(ethylene glycol)/poly(octadecanedioic anhydride) amphiphilic triblock co-polymer nanoparticles.a family of injectable, biodegradable and thermosensitive co-polymer nanoparticle (np) hydrogels based on mpeg-b-poa-b-mpeg, which was synthesized from mono-methoxy poly(ethylene glycol) (mpeg) and poly(octadecanedioic anhydride) (poa), was investigated in this paper. it was found that the aqueous dispersions of these nps underwent a reversible gel-sol transition upon temperature change. by using paclitaxel and bovine serum albumin (bsa) as model drugs, we noticed that the in vitro releases of b ...201121294968
seasonal changes in courtship behavior, plasma androgen levels and in hypothalamic aromatase immunoreactivity in male free-living european starlings (sturnus vulgaris).in songbirds from temperate latitudes, singing during spring has an essential role in mate attraction, while during the non-breeding season it is connected to territorial aggression and/or maintaining dominance hierarchies or flock cohesion. courtship behavior is regulated by plasma testosterone (t) levels. other androgens, like dehydroepiandrosterone (dhea) could be responsible for aggression. the aromatization of androgens in the brain is an essential step in mediating their effects on behavio ...201121310152
forage as a primary source of mycotoxins in animal diets.the issue of moulds and, thus, contamination with mycotoxins is very topical, particularly in connexion with forages from grass stands used at the end of the growing season. deoxynivalenol (don), zearalenone (zea), fumonisins (fum) and aflatoxins (afl) are among the most common mycotoxins. the aim of the paper was to determine concentrations of mycotoxins in selected grasses (lolium perenne, festulolium pabulare, festulolium braunii) and their mixtures with festuca rubra an/or poa pratensis duri ...201021318013
foreign born status and hiv/aids: a comparative analysis of hiv/aids characteristics among foreign and u.s. born individuals.the purpose of this study was to observe differences in hiv characteristics between the foreign and u.s. born hiv population. the study sample consisted of individuals, =13 years of age, who have been diagnosed with hiv and are patients of the lexington bluegrass care clinic. for the comparison analysis, the sample was divided into two groups (n = 1070), foreign born and u.s. born. compared to u.s. born patients, foreign born patients were younger at the time of their hiv diagnosis (31 years vs. ...201121327966
insulin-like growth factor 1-mediated hyperthermia involves anterior hypothalamic insulin receptors.the objective is to investigate the role of insulin-like growth factor 1 (igf-1) in the regulation of core body temperature. sequencing cdna libraries from individual warm-sensitive neurons from the preoptic area (poa) of the hypothalamus, a region involved in the central control of thermoregulation, identified neurons that express both igf-1 receptor (igf-1r) and insulin receptor transcripts. the effects of administration of igf-1 into the poa was measured by radiotelemetry monitoring of core t ...201121330367
determination of oxidant stress in plasma of rheumatoid arthritis and primary osteoarthritis patients.determination of oxidant stress in plasma of rheumatoid arthritis (ra) and primary osteoarthritis (poa) patients is important in understanding the pathogenesis of these diseases. in this study, we examined the relationship between oxidant stress and inflammation by measuring protein carbonyl content, thiol levels and plasma protein fractions as the oxidation markers and erythrocyte sedimentation rate (esr), c-reactive protein (crp) tests as inflammation markers. protein carbonyls content was hig ...201021355418
volatile constituents of festuca nigrescens, phleum alpinum and poa alpina from n. w. italian alpine pastures.the composition of the volatile fractions of three important grasses from sub-alpine n.w. italian pastures, namely festuca nigrescens lam. non gaudin (chewing fescue), phleum alpinum l. (alpine timothy) and poa alpina l. (alpine bluegrass) was investigated. the fresh aerial parts were collected at the flowering stage during the summer season. the volatile oils obtained from green tissues by steam distillation in a clevenger-type apparatus, were analyzed by gc/fid and gc/ms. the oil yield was 0.0 ...201121366056
sex differences in the rapid control of aromatase activity in the quail preoptic area.adult male quail show high levels of aromatase activity in the preoptic area-hypothalamus (poa-hyp), which parallels the high number of aromatase-immunoreactive cells and elevated mrna concentrations detected in this brain region by in situ hybridisation. interestingly, females display considerably lower aromatase activity than males but have almost equal numbers of aromatase-immunoreactive cells and express similar levels of aromatase mrna. aromatase activity in the male poa-hyp can be rapidly ...201121366731
intracranial study of speech-elicited activity on the human posterolateral superior temporal gyrus.to clarify speech-elicited response patterns within auditory-responsive cortex of the posterolateral superior temporal (plst) gyrus, time-frequency analyses of event-related band power in the high gamma frequency range (75-175 hz) were performed on the electrocorticograms recorded from high-density subdural grid electrodes in 8 patients undergoing evaluation for medically intractable epilepsy. stimuli were 6 stop consonant-vowel (cv) syllables that varied in their consonant place of articulation ...201121368087
elevated estrogen receptor expression in hypothalamic preoptic area decreased by electroacupuncture in ovariectomized rats.in the present study, effects of electroacupuncture (ea) on estrogen receptor alpha (era) and beta (erß) mrna and protein expression in the hypothalamus of ovariectomized (ovx) rats were detected by quantitative real-time reverse transcription pcr (qrt-pcr) and western blot analysis. gonadotropin-releasing hormone (gnrh) release and gnrh mrna level in hypothalamic preoptic area (poa) were evaluated by push-pull perfusion and qrt-pcr. our results showed that elevated mrna and protein expression o ...201121376784
an exploration of the utility of mathematical modeling predicting fatigue from sleep/wake history and circadian phase applied in accident analysis and prevention: the crash of comair flight 5191.on 27 august 2006 at 0606 eastern daylight time (edt) at bluegrass airport in lexington, ky (lex), the flight crew of comair flight 5191 inadvertently attempted to take off from a general aviation runway too short for their aircraft. the aircraft crashed killing 49 of the 50 people on board. to better understand this accident and to aid in preventing similar accidents, we applied mathematical modeling predicting fatigue-related degradation in performance for the air traffic controller on-duty at ...201121376901
sleep-waking discharge profiles of median preoptic and surrounding neurons in mice.the median preoptic nucleus (mnpo), part of the anteroventral third ventricular region, plays a key role in body fluid homeostasis and cardiovascular regulation. recently, a cluster of neurons showing sleep-related c-fos immunoreactivity was found in the rat mnpo, and a subsequent electrophysiological study found that nearly 76% of rat mnpo neurons exhibit increased discharge during sleep. in a recent single unit recording study in mice, we found that sleep-active neurons are not localized in an ...201121396987
manganese induces igf-1 and cyclooxygenase-2 gene expressions in the basal hypothalamus during prepubertal female development.precocious puberty is a significant child health problem, especially in girls, because 95% of cases are idiopathic. our earlier studies demonstrated that low-dose levels of manganese (mn) caused precocious puberty via stimulating the secretion of luteinizing hormone-releasing hormone (lhrh). because glial-neuronal communications are important for the activation of lhrh secretion at puberty, we investigated the effects of prepubertal mn exposure on specific glial-derived puberty-related genes kno ...201121402727
cold-induced responses in annual bluegrass genotypes with differential resistance to pink snow mold (microdochium nivale).greens-type annual bluegrass (poa annua l.) is susceptible to winter stresses including subfreezing temperatures and pink snow mold (sm). to better understand the mechanisms of sm resistance in annual bluegrass, four sm-resistant and four sm-sensitive genotypes were incubated at low temperature with microdochium nivale (fries) samuels & hallett, the causal agent of pink snow mold. we assessed the impact of a 6-week incubation period with sm at 2 °c under high humidity (≥ 98%) on the accumulation ...201121421353
a next-generation sequencing method for overcoming the multiple gene copy problem in polyploid phylogenetics, applied to poa grasses.polyploidy is important from a phylogenetic perspective because of its immense past impact on evolution and its potential future impact on diversification, survival and adaptation, especially in plants. molecular population genetics studies of polyploid organisms have been difficult because of problems in sequencing multiple-copy nuclear genes using sanger sequencing. this paper describes a method for sequencing a barcoded mixture of targeted gene regions using next-generation sequencing methods ...201121429199
[the origin of polyploid genomes of bluegrasses poa l. and gene flow between northern pacific and sub-antarctic islands].the involvement of present-day diploid bluegrass species in the formation of polyploid genomes was investigated using comparison of sequences of internal transcribed spacers its1 and its2, and the 5.8s rdna sequence. it was demonstrated that highly polyploid new zealand bluegrasses, p. cita (2n = 84; ca. 96 to 100), p. chathamica (2n = 112), and p. litorosa (2n = 263 to 266) formed separate highly supported clade together with tetraploids (2n = 28) p. intrusa, p. anceps, and p. trioides (austrof ...201021434413
release kinetics of ß-cyfluthrin from its encapsulated formulations in water.controlled release formulations of ß-cyfluthrin, a non-systemic, broad spectrum contact insecticide, have been prepared using laboratory synthesized poly(ethylene glycol) (peg) based amphiphilic copolymers. copolymers of polyethylene glycols of different molecular weights and various dimethyl esters, viz. dimethyl isophthalate, which self assemble into nano micellar aggregates in aqueous media, have been synthesized. the kinetics of ß-cyfluthrin from developed controlled release (cr) formulation ...201121442535
native perennial grasses show evolutionary response to bromus tectorum (cheatgrass) invasion.invasive species can change selective pressures on native plants by altering biotic and abiotic conditions in invaded habitats. although invasions can lead to native species extirpation, they may also induce rapid evolutionary changes in remnant native plants. we investigated whether adult plants of five native perennial grasses exhibited trait shifts consistent with evolution in response to invasion by the introduced annual grass bromus tectorum l. (cheatgrass), and asked how much variation the ...201121479185
validity of the ahrq patient safety indicator "central venous catheter-related bloodstream infections"."central venous catheter-related bloodstream infections" (cr-bsis) is one of the patient safety indicators (psi 7) developed by the agency for healthcare research and quality (ahrq) to screen for potential safety events. we sought to investigate the validity of this psi using the medical record as the gold standard.201121489833
prostaglandin e₂ regulates ampa receptor phosphorylation and promotes membrane insertion in preoptic area neurons and glia during sexual differentiation.sexual differentiation of the rodent brain is dependent upon the organizing actions of the steroid hormone, estradiol. in the preoptic area, a brain region critical for the expression of adult reproductive behavior, there are twice as many dendritic spine synapses per unit length on newborn male neurons compared to female neurons and this sex difference correlates with the expression of adult male copulatory behavior. the sex difference in the poa is achieved via estradiol's upregulation of the ...201121490976
nuclear magnetic resonance-based metabonomic study of early time point laminitis in an oligofructose-overload model.reasons for performing study: nmr-metabonomics is an unbiased evaluation method, which allows to comprehensively study changes of the equine metabolic profile in early time point laminitis. this might give insight into the early stages of disease development. objectives: to detect hitherto unknown changes in blood metabolites during the development of oligofructose-induced laminitis by comparing pre- and post induction blood samples. methods: prior to laminitis induction blood was sampled to est ...201121496096
venous thromboembolism prophylaxis in surgical patients: identifying a patient group to maximize performance improvement.venous thromboembolism (vte) in surgical patients is a cause of increased morbidity, mortality, and cost of care. deep vein thrombosis (dvt) prophylaxis reduces the risk of dvt or pulmonary embolism (pe), but not to zero, which is concerning because dvt/pe is being considered a serious reportable event. a study was conducted in january-june 2009 to test the hypothesis that most surgical vtes occur despite the patient's receiving appropriate prophylaxis.201121500718
establishing a comprehensive networkwide pressure ulcer identification process.pressure ulcers (pus) are a critical concern, endangering patients and requiring significant resources for treatment in stage ii/iv. the centers for medicare & medicaid services (cms) denies reimbursement in cases where a more complex diagnosis-related group (drg) is assigned as a result of hospital-acquired conditions such as a pu that could have been reasonably prevented.201121500756
pharmaceutical opioid analgesic and heroin dependence: how do treatment-seeking clients differ in australia?introduction and aims. non-prescribed use of pharmaceutical opioid analgesics (poa) has been escalating internationally. in australia, few studies have examined if poa users have similar characteristics and treatment needs to heroin users. the aim of this study was to compare those presenting for treatment where heroin versus poa were the primary drugs of concern. design and methods. a convenience sample of 192 treatment entrants were recruited from alcohol and drug treatment services in four au ...201121545560
immunocytochemical localization of 11 beta-hydroxysteroid dehydrogenase in hippocampus and other brain regions of the rat.the dehydrogenase form of 11 β-hydroxysteroid dehydrogenase (11-dh) which catalyzes the oxidation of the biologically active steroid, corticosterone, to its inactive metabolite, 11-dehydrocorticosterone, is found in rat brain. the distribution and localization of 11-dh-like labeling in the rat brain was examined by immunocytochemistry. 11-dh-like immunostaining was found in all subfields of the hippocampus and in many other parts of the brain, including the preoptic area (poa), central nucleus o ...199221554583
crf neurogenesis during midlife development in salmon: genetic, environmental and thyroid hormone regulation.salmon parr-smolt transformation (smoltification) is a mid-life transitional stage between life in freshwater and seawater that entails a wide range of neural, endocrine and physiological modifications. in salmon, the neuroendocrine corticotropin releasing factor (crf) system regulates pituitary acth and tsh release. four experimental groups of atlantic salmon, salmo salar, were used to investigated crf neurogenesis and its regulation during smoltification. we compared 1) developmental stages (p ...201121592238
central injection of exogenous il-1β in the control activities of hypothalamic-pituitary-gonadal axis in anestrous ewes.this study was performed to determine the effect of intracerebroventricular (icv) injection of interleukin (il)-1β on the gene expression, translation and release of gonadotropin-releasing hormone (gnrh) and the gnrh receptor (gnrhr) gene expression in the hypothalamus of anestrous ewes. in the anterior pituitary gland (ap), the expression of genes encoding: gnrhr, β subunits of luteinizing hormone (lh) and folliculotropic hormone (fsh) was determined as well as the effect of il-1β on pituitary ...201121595758
galanin neurons in the intermediate nucleus (inm) of the human hypothalamus in relation to sex, age and gender identity.the intermediate nucleus (inm) in the preoptic area of the human brain, also known as the sexually dimorphic nucleus of the preoptic area (sdn-poa) and the interstitial nucleus of the anterior hypothalamus-1 (inah-1) has been studied. we investigated its population of galanin-immunoreactive (gal-ir) neurons in relation to sex, age and gender identity in the postmortem brain of 77 subjects. first we compared the inm volume and number of gal-ir neurons of 22 males and 22 females in the course of a ...201121618223
protein supplementation of ruminants consuming low-quality cool- or warm-season forage: differences in intake and digestibility.an in situ study (exp. 1) using 4 ruminally cannulated steers (343 ± 11 kg bw) in a completely randomized design was used to compare ruminal degradation characteristics of low-quality cool-season (c3; kentucky bluegrass straw; poa pratensis; 6.3% cp; dm basis) and warm-season (c4; tallgrass prairie; 5.7% cp; dm basis) forage. four ruminally cannulated steers (252 ± 8 kg bw; exp. 2) and 4 wethers (38 ± 1 kg bw; exp. 3) were used in two, 2 × 2 factorial design experiments to determine the influenc ...201121622884
regulation of the female rat estrous cycle by a neural cell-specific epidermal growth factor-like repeat domain containing protein, nell2.nell2, a protein containing epidermal growth factor-like repeat domains, is predominantly expressed in the nervous system. in the mammalian brain, nell2 expression is mostly neuronal. previously we found that nell2 is involved in the onset of female puberty by regulating the release of gonadotropin-releasing hormone (gnrh), and in normal male sexual behavior by controlling the development of the sexually dimorphic nucleus of the preoptic area (poa). in this study we investigated the effect of ne ...201121643849
a new epichloe species with interspecific hybrid origins from poa pratensis ssp. pratensis in liyang, china.we describe a new epichloë species found in symbiosis with poa pratensis ssp. pratensis in liyang, china. stromata characteristic of epichloë spp. were present on some of the reproductive tillers of individual host grasses. only 3 of the 98 stromata observed on field plants became orange and produced perithecia. phylogenetic analyses based on sequences of tubb and tefa indicated that this epichloë sp. was an interspecific hybrid related to both e. yangzii and members in the e. typhina complex ...201121659456
histological characterization of gonadotropin-releasing hormone (gnrh) in the hypothalamus of the south american plains vizcacha (lagostomus maximus).in contrast to most mammalian species, females of the south american plains vizcacha, lagostomus maximus, show an extensive suppression of apoptosis-dependent follicular atresia, continuous folliculogenesis, and massive polyovulation. these unusual reproductive features pinpoint to an eventual peculiar modulation of the hypothalamo-hypophyseal-gonadal axis through its main regulator, the gonadotropin-releasing hormone (gnrh). we explored the hypothalamic histological landscape and cellular and s ...201121660456
persistence of seed bank under thick volcanic deposits twenty years after eruptions of mount usu, hokkaido island, japan.the topsoil that contained the seed bank became buried under thick tephra after the eruptions of mount usu during 1977 and 1978. to determine the seed bank potential of the topsoil 20 yr after the eruptions, i.e., in 1998, 408 100-cm(3) samples were excavated under 115-185 cm of volcanic deposits. the topsoil was collected at 10-cm intervals along the horizontal scale and was divided into a 0-5 cm deep upper layer and a 5-10 cm deep lower layer. the seed bank was estimated by both the germinatio ...200121669614
leaf shape and anatomy as indicators of phase change in the grasses: comparison of maize, rice, and bluegrass.leaf morphology and anatomy during vegetative phase change was compared in bluegrass, rice, and maize. maize juvenile leaves are coated with epicuticular wax, lack specialized cells, such as trichomes and bulliform cells, and epidermal cell walls stain a uniform purple color. adult maize leaves are pubescent, lack epicuticular waxes, and have crenulated epidermal cell walls that stain purple and blue. all bluegrass and rice blades are pubescent, coated with epicuticular waxes, and show purple an ...200121669648
[effect of femoral offset reconstruction on pelvic stability during gait after total hip arthroplasty].to investigate the effect of femoral offset reconstruction on pelvic stability during gait after total hip arthroplasty.201121675102
developmental changes in hypothalamic kiss1 expression during activation of the pulsatile release of luteinising hormone in maturing ewe lambs.onset of puberty is characterised by a marked increase in the frequency of release of gonadotrophin-releasing hormone (gnrh) and luteinising hormone (lh). the kiss1 gene plays a critical role in pubertal development, and its product, kisspeptin, stimulates gnrh and lh release. in the present study, we tested the hypothesis that kiss1 gene expression in the preoptic area (poa) and hypothalamus increases during maturation of the reproductive neuroendocrine axis in association with increased lh pul ...201121679258
within-population genetic diversity of taraxacum officinale (asteraceae): differential genotype response and effect on interspecific competition.clonal offspring of five morphologically distinct individuals of taraxacum officinale were planted in a greenhouse experiment with each of three competitors, plantago major, poa pratensis and trifolium pratense. the competitors were chosen to represent a series of competitive environments experienced by a natural population of t. officinale through the year. differences in size, morphology, and response to the competitive environments were found among clones and support classification of the fiv ...199821684978
evidence that the arcuate nucleus is an important site of progesterone negative feedback in the ewe.there is now considerable evidence that dynorphin neurons mediate the negative feedback actions of progesterone to inhibit gnrh and lh pulse frequency, but the specific neurons have yet to be identified. in ewes, dynorphin neurons in the arcuate nucleus (arc) and preoptic area (poa) are likely candidates based on colocalization with progesterone receptors. these studies tested the hypothesis that progesterone negative feedback occurs in either the arc or poa by determining whether microimplants ...201121693677
effects of mycorrhizae on plant growth and dynamics in experimental tall grass prairie microcosms.experimental microcosms (40 x 52 x 32 cm) containing an assemblage of eight tallgrass prairie grass and forb species in native prairie soil were maintained under mycorrhizal (untreated control) or mycorrhizal-suppressed (fungicide-treated) conditions to examine plant growth, demographic, and community responses to mycorrhizal symbiosis. the fungicide benomyl successfully reduced mycorrhizal root colonization in the fungicide-treated microcosms to only 6.4% (an 83% reduction relative to mycorrhiz ...199721708601
evaluation of hospitalization for infections that are present on admission.hospitals have experienced increasing requirements for public reporting of various infection rates using clinical and administrative data. until recently, such reports have not included analysis of "present on admission" (poa), an indicator designed to assess whether such infections are hospital acquired. the authors evaluated the frequency of the poa coding designation for 167 university healthsystem consortium hospitals for sepsis/septicemia (s-s), methicillin-resistant staphylococcus aureus ( ...201121724961
metal contamination of soils and plants associated with the glass industry in north central india: prospects of phytoremediation.introduction: the effect of the glass industry on urban soil metal characterization was assessed in the area of firozabad, india. a comprehensive profile of metal contamination was obtained in five zones each containing five specific sites. findings: zn, cd, and as showed a greater accumulation, whereas accumulation of ni and cu was high in limited samples. positive correlation was found for the metal pairs cu-zn, cu-co, and cu-cr at pôçë<ôçë0.01. moderate positive correlation was also observed ...201121735162
pyrazinamide, but not pyrazinoic acid, is a competitive inhibitor of nadph binding to mycobacterium tuberculosis fatty acid synthase i.pyrazinamide (pza), an essential component of short-course anti-tuberculosis chemotherapy, was shown by saturation transfer difference (std) nmr methods to act as a competitive inhibitor of nadph binding to purified mycobacterium tuberculosis fatty acid synthase i (fas i). both pza and pyrazinoic acid (poa) reversibly bind to fas i but at different binding sites. the competitive binding of pza and nadph suggests potential fas i binding sites. poa was not previously known to have any specific bin ...201121775138
effect of hydrophilic organic seed aerosols on secondary organic aerosol formation from ozonolysis of a-pinene.gas-particle partitioning theory is widely used in atmospheric models to predict organic aerosol loadings. this theory predicts that secondary organic aerosol (soa) yield of an oxidized volatile organic compound (voc) product will increase as the mass loading of preexisting organic aerosol increases. in a previous study, we showed that the presence of model hydrophobic primary organic aerosol (poa) had no detectable effect on the soa yields from ozonolysis of a-pinene, suggesting that the conden ...201121790137
neurons of the lateral preoptic area/rostral anterior hypothalamic area are required for photoperiodic inhibition of estrous cyclicity in female sheep.photoperiod determines the timing of reproductive activity in many species, yet the neural pathways whereby daylength is transduced to a signal influencing gnrh release are not fully understood. physical lesions of the lateral preoptic area (lpoa)/rostral anterior hypothalamic area (raha) in female sheep extend the period of estrous cyclicity during inhibitory photoperiods. herein we sought to determine if destroying only neurons and not fibers of passage in this area would lead to similar resis ...201121816852
pyrazinamide inhibits trans-translation in mycobacterium tuberculosis.pyrazinamide (pza) is a first-line tuberculosis drug that plays a unique role in shortening the duration of tuberculosis chemotherapy. pza is hydrolyzed intracellularly to pyrazinoic acid (poa) by pyrazinamidase (pzase, encoded by pnca), an enzyme frequently lost in pza-resistant strains, but the target of poa in mycobacterium tuberculosis has remained elusive. here, we identify a new target of poa as the ribosomal protein s1 (rpsa), a vital protein involved in protein translation and the riboso ...201121835980
Interactive effects of plant-available soil silicon and herbivory on competition between two grass species.The herbivore defence system of true grasses (Poaceae) is predominantly based on silicon that is taken up from the soil and deposited in the leaves in the form of abrasive phytoliths. Silicon uptake mechanisms can be both passive and active, with the latter suggesting that there is an energetic cost to silicon uptake. This study assessed the effects of plant-available soil silicon and herbivory on the competitive interactions between the grasses Poa annua, a species that has previously been repo ...201121868406
[Sustained release of the antitumor drug paclitaxel from poly(3-hydroxybutyrate)-based microspheres].Development of systems of medicines with sustained action on the basis of biodegradable polymers is a promising trend in modem pharmacology. Polyhydroxyalkanoates (POA) attract increasing attention due to their biodegradability and high biocompatibility, which make them suitable for development of novel drug dosage forms. We obtained microspheres on the basis of poly(3-hydroxybutyrate) (PHB) loaded with the antitumor drug paclitaxel. Morphology, drug release kinetics and effect on tumor cells in ...201121870610
socioeconomic distinction, cultural tastes, and cigarette smoking.objectives: the inverse relationship between socioeconomic status (ses) and smoking is typically seen in terms of the greater economic and social resources of advantaged groups, but it may also relate to cultural resources. this study aims to test theories of symbolic distinction by examining relationships between smoking and ostensibly unrelated cultural preferences. methods: using the 1993 general social survey, ordinal logistic regression models, and a three-category dependent variable (never ...200621874073
Testing the priority-of-access model in a seasonally breeding primate species.In mammals, when females are clumped in space, male access to receptive females is usually determined by a dominance hierarchy based on fighting ability. In polygynandrous primates, as opposed to most mammalian species, the strength of the relationship between male social status and reproductive success varies greatly. It has been proposed that the degree to which paternity is determined by male rank decreases with increasing female reproductive synchrony. The priority-of-access model (PoA) pred ...201121874084
Central circuitries for body temperature regulation and fever.Body temperature regulation is a fundamental homeostatic function that is governed by the central nervous system in homeothermic animals, including humans. The central thermoregulatory system also functions for host defense from invading pathogens by elevating body core temperature, a response known as fever. Thermoregulation and fever involve a variety of involuntary effector responses, and this review summarizes the current understandings of the central circuitry mechanisms that underlie nonsh ...201121900642
Altered position of cell bodies and fibers in the ventromedial region in SF-1 knockout mice.The ventromedial nucleus of the hypothalamus (VMH) is a key cell group in the medial-basal hypothalamus that participates in the regulation of energy balance. Previous studies have shown that the cellular organization of the VMH is altered in mice with a disruption of the steroidogenic factor-1 (NR5a1) gene (SF-1 KO mice). The present study examined orexigenic/anorexigenic peptides (neuropeptide Y (NPY), agouti-related peptide (AgRP) and cocaine- and amphetamine-regulated transcript (CART)) and ...201121906594
Glycoproteins are species-specific markers and major IgE reactants in grass pollens.Grass pollen allergic patients are concomitantly exposed and sensitized to pollens from multiple Pooideae (i.e. common grass) species. As such, they are currently desensitized by allergen-specific immunotherapy using extracts made from mixes of pollens from Anthoxanthum odoratum, Dactylis glomerata, Lolium perenne, Phleum pratense and Poa pratensis. Herein, we demonstrate that species-specific glycoprotein patterns are documented by 1D and 2D electrophoresis and Western blotting analysis, which ...201121951299
Genome-wide examination of the transcriptional response to ecdysteroids 20-hydroxyecdysone and ponasterone A in Drosophila melanogaster.The 20-hydroxyecdysone (20E) hierarchy of gene activation serves as an attractive model system for studying the mode of steroid hormone regulated gene expression and development. Many structural analogs of 20E exist in nature and among them the plant-derived ponasterone A (PoA) is the most potent. PoA has a higher affinity for the 20E nuclear receptor, composed of the ecysone receptor (EcR) and Ultraspiracle proteins, than 20E and a comparison of the genes regulated by these hormones has not bee ...201121958154
Constitutive heat shock protein 70 interacts with a-enolase and protects cardiomyocytes against oxidative stress.Constitutive heat shock protein 70 (Hsc70) is a molecular chaperone that has been shown to protect cardiomyocytes against oxidative stress. However, the molecular mechanism responsible for this protection remains uncertain. To understand the mechanism associated with the myocardial protective role of Hsc70, we have embarked upon a systematic search for Hsc70-interacting proteins. Using adenosine diphosphate (ADP) affinity chromatography and mass spectrometry, we have identified a-enolase, a rate ...201121958194
lc/ms/ms identification of 20-hydroxyecdysone in a scorpion (liocheles australasiae) and its binding affinity to in vitro-translated molting hormone receptors.recent advances in mass spectrometry (ms) technology have facilitated the detection and quantification of minor components in organisms and the environment. in this study, we successfully identified 20-hydroxyecdysone (20e) in first instar nymphs (7 days after hatching) of the scorpion liocheles australasiae, using tandem mass spectrometry combined with high-performance liquid chromatography (lc/ms/ms). this substance was not found in adults after the fifth stage. other possible molting hormone ...201121958716
uptake of heavy metals by native species growing in a mining area in sardinia, italy: discovering native flora for phytoremediation.this study assessed the distribution and availability of plant uptake of zn, pb, and cd present in an abandoned mine at ingurtosu, sardinia (italy). geological matrix samples (sediments, tailings, and soil from a nearby pasture site) and samples of the predominant plant species growing on sediments and tailings were collected. mean values of total zn, pb and cd were respectively (mg kg(-1)) 7400, 1800, and 56 in tailings, 31000, 2900, and 100 in sediments, and 400, 200, and 8 in the pasture soil ...201121972566
vegetative reproduction capacities of floodplain willows - cutting response to competition and biomass loss.while several studies on regeneration in salicaceae have focused on seedling recruitment, little is known about factors controlling their vegetative reproduction. in two greenhouse experiments, we studied the response of floodplain willows (salix fragilis, s. viminalis, s. triandra) to competition with poa trivialis, and to shoot and root removal when planted as vegetative cuttings. in the first experiment, growth performance variables were analysed in relation to full competition, shoot competi ...201121972956
both olfactory epithelial and vomeronasal inputs are essential for activation of the medial amygdala and preoptic neurons of male rats.chemosensory inputs signaling volatile and nonvolatile molecules play a pivotal role in sexual and social behavior in rodents. we have demonstrated that olfactory preference in male rats, that is, attraction to receptive female odors, is regulated by the medial amygdala (mea), the cortical amygdala (coa), and the preoptic area (poa). in this paper, we investigated the involvement of two chemosensory organs, the olfactory epithelium (oe) and the vomeronasal organ (vno), in olfactory preference an ...201121983295
Kisspeptin neurons mediate reflex ovulation in the musk shrew (Suncus murinus).The present study investigated whether kisspeptin-G protein-coupled receptor 54 (GPR54) signaling plays a role in mediating mating-induced ovulation in the musk shrew (Suncus murinus), a reflex ovulator. For this purpose, we cloned suncus Kiss1 and Gpr54 cDNA from the hypothalamus and found that suncus kisspeptin (sKp) consists of 29 amino acid residues (sKp-29). Injection of exogenous sKp-29 mimicked the mating stimulus to induce follicular maturation and ovulation. Administration of several ki ...201121987818
validating the patient safety indicators in the veterans health administration: do they accurately identify true safety events?the agency for healthcare research and quality (ahrq) patient safety indicators (psis) use administrative data to detect potentially preventable in-hospital adverse events. however, few studies have determined how accurately the psis identify true safety events.201221993057
AdipoR1 and 2 are expressed on warm sensitive neurons of the hypothalamic preoptic area and contribute to central hyperthermic effects of adiponectin.Adiponectin can act in the brain to increase energy expenditure and reduce body weight by mechanisms not entirely understood. We found that adiponectin type 1 and type 2 receptors (AdipoR1 and AdipoR2) are expressed in warm sensitive neurons of the hypothalamic preoptic area (POA) which play a critical role in the regulation of core body temperature (CBT) and energy balance. Thus, we tested the ability of adiponectin to influence CBT in wild-type mice and in mice deficient for AdipoR1 or AdipoR2 ...201122000082
early life exposure to endocrine-disrupting chemicals causes lifelong molecular reprogramming of the hypothalamus and premature reproductive aging.gestational exposure to the estrogenic endocrine disruptor methoxychlor (mxc) disrupts the female reproductive system at the molecular, physiological, and behavioral levels in adulthood. the current study addressed whether perinatal exposure to endocrine disruptors reprograms expression of a suite of genes expressed in the hypothalamus that control reproductive function and related these molecular changes to premature reproductive aging. fischer rats were exposed daily for 12 consecutive days to ...201122016562
Variation in Gonadotropin Releasing-Hormone-1 Gene Expression in the Preoptic Area Predicts Transitions in Seasonal Reproductive State.In many seasonally reproducing animals, the experience of prolonged exposure to constant photoperiods results in the induction of a state of photorefractoriness, defined as a lack of responsiveness to a previously stimulatory photoperiod. The physiological and genetic processes that control photorefractoriness are not well understood, however, the hallmark of photorefractoriness is an endogenous change in the physiological response to a constant photoperiod. It is already known that preoptic are ...201122023598
prairie grass establishment on calcareous reclaimed mine soil.reclaimed appalachian surface mined lands have difficulty in sustaining native deciduous forest communities. establishing prairie communities could increase ecosystem function; however, a native model system does not exist. we evaluated establishment of 15 north american prairie grasses as monocultures on reclaimed mine soil in southeast ohio in four randomized complete blocks planted may 2005 and 2006. population density was assessed 30 d after planting (30 dap) and in october of the planting y ...201122031565
tetra-aqua-bis-(2-{[5-(pyridin-4-yl)-1,3,4-oxadiazol-2-yl]sulfan-yl}acetato)-iron(ii).in the title compound, [fe(c(9)h(6)n(3)o(3)s)(2)(h(2)o)(4)] or [fe(poa)(2)(h(2)o)(4)], the fe(ii) atom is located on an inversion center and is ligated by four o atoms of coordinated water mol-ecules in the equatorial plane while two poa ligands acting as monodentate ligands occupy the axial positions through their pyridyl n atoms, completing a slightly distorted octa-hedral coordination geometry. a three-dimensional supra-molecular network is formed by multiple o-h⋯o hydrogen-bonding inter-acti ...201122058719
sire variation in fatty acid composition of crossbred wagyu steers and heifers.effects of sires on lipid composition of subcutaneous adipose tissue and longissimus dorsi muscle were studied using 113 f(1) heifer and steer calves sired by eight wagyu bulls out of three different cow herds. wagyu sires were used and grouped as old (n=6) and new (n =2) sires, respectively, based on the chronological order in which they were imported into the us. animals were fed a backgrounding diet for 112 days consisting of an 80:20 ratio of roughage:concentrate, then grazed on orchard gras ...200022061767
Composition and hydrothermal pretreatment and enzymatic saccharification performance of grasses and legumes from a mixed-species prairie.ABSTRACT:201122085451
a wide diversity of cortical gabaergic interneurons derives from the embryonic preoptic area.gaba-containing (gabaergic) interneurons comprise a very heterogeneous group of cells that are crucial for cortical function. different classes of interneurons specialize in targeting specific subcellular domains of excitatory pyramidal cells or other interneurons, which provides cortical circuits with an enormous capability for information processing. as in other regions of the cns, cortical interneuron diversity is thought to emerge from the genetic specification of different groups of progeni ...201122090484
The accuracy of present-on-admission reporting in administrative data.To test the accuracy of reporting present-on-admission (POA) and to assess whether POA reporting accuracy differs by hospital characteristics.201122092023
Simulating the Degree of Oxidation in Atmospheric Organic Particles.Modeled ratios of organic mass to organic carbon (OM/OC) and oxygen to carbon (n(O)/n(C)) in organic particulate matter are presented across the US for the first time and evaluated extensively against ambient measurements. The base model configuration systematically underestimates OM/OC ratios during winter and summer months. Model performance is greatly improved by applying source-specific OM/OC ratios to the primary organic aerosol (POA) emissions and incorporating a new parametrization to sim ...201122107341
bilateral persistent primitive trigeminal arteries associated with trigeminal neuralgia.persistent carotid-vertebrobasilar anastomoses (pcvba) include the primitive trigeminal artery (pta), the primitive otic artery (poa), the primitive hypoglossal artery and proatlantal arteries (proas). the pta is the most commonly seen of these accounting for approximately 80-85% of pcvbas. the pta which connects the internal carotid artery (ica) to the basilar artery (ba) may occasionally connect to the superior or posterior inferior cerebellar arteries without interposition to the ba. it is th ...201122113402
Adverse Outcomes in Patients with Postoperative Ascites after Liver Resection for Hepatocellular Carcinoma.BACKGROUND: Postoperative ascites (POA) is a common complication after liver resection (LR). The aim of the present study was to identify the risk factors for developing POA and to evaluate its clinical significance in the prognosis of patients with hepatocellular carcinoma (HCC). METHODS: We performed a retrospective analysis of data obtained from 651 patients who underwent LR for the treatment of HCC between Ja ...201122131090
cyanide poisoning of cattle grazing "reed sweet-grass"abstract extract reed sweet-grass, poa aquatica (glyceria maxima, glyceria acquatica), a member of the gramineae family, is an introduced plant found in wet places in various localities in both the north and south islands, but mainly in otago and southland (allan, 1940 ). garner ( 1961 , p. 318) quotes quisumbine (1947) as listing 25 species belonging to the family gramineae, including poa aquatica, which contain hydrocyanic acid. connor (1951) , however, does not list poa aquatica as on ...197622133056
Sacropelvic Fixation With Iliosacral Screws: Applications and Results in Adult Spinal Deformities.STUDY DESIGN:: Retrospective radiographic and clinical review. OBJECTIVE:: To determine the feasibility of iliosacral screw fixation in adult spinal deformations. SUMMARY OF BACKGROUND DATA:: Pelvic fixation is one of the most challenging instrumentation problems. The poor bone quality frequently found within the sacrum and the large lumbosacral loads with cantilever pullout forces across this region explain its frequent failure. METHODS:: Fourteen adult patients undergoing pelvic fixation using ...201122134734
Hydrated phosphorus oxyacids alone and adsorbed on nanosilica.Low-temperature (1)? NMR spectroscopy was used to study states of water bound to phosphoric and phosphonic acids (phosporus oxyacids, POA) alone or adsorbed onto nanosilica OX-50 (specific surface area S(BET)=52m(2)/g) or A-300 (S(BET)=297m(2)/g). Concentrated solutions or weakly hydrated solid POA or dried silica/POA powders placed in CCl(4) medium are characterized by different temperature dependences of the chemical shift of the proton resonance (d(H)) because of partial dissociation of PO-H ...201122138264
comparison of different gas-phase mechanisms and aerosol modules for simulating particulate matter formation.the effects of two gas-phase chemical kinetic mechanisms, regional atmospheric chemistry mechanism version 2 (racm2) and carbon-bond 05 (cb05), and two secondary organic aerosol (soa) modules, the secondary organic aerosoi model (sorgam) and aer/epri/caltech model (aec), on fine (aerodynamic diameter < or =2.5 microm) particulate matter (pm2.5) formation is studied. the major sources of uncertainty in the chemistry of soa formation are investigated. the use of all major soa precursors and the tr ...201122168105
Bidirectional EphrinB3/EphA4 Signaling Mediates the Segregation of Medial Ganglionic Eminence- and Preoptic Area-Derived Interneurons in the Deep and Superficial Migratory Stream.The integration of interneuron subtypes into specific microcircuits is essential for proper cortical function. Understanding to what extent interneuron diversity is regulated and maintained during development might help to reveal the principles that govern their role as synchronizing elements as well as causes for dysfunction. Particular interneuron subtypes are generated in a temporally regulated manner in the medial ganglionic eminence (MGE), the caudal ganglionic eminence, and the preoptic ar ...201122171039
variation in enkephalin immunoreactivity in the social behavior network and song control system of male european starlings (sturnus vulgaris) is dependent on breeding state and gonadal condition.many temperate zone songbird species exhibit marked seasonal variation in song quality as well as in the motivation to sing. two brain systems are known to mediate such annual variation in song quality and motivation: (1) the song control system (scs), and (2) the social behavior network (sbn), respectively. how these two circuits interact to produce changes in singing behavior is not well understood. the opioid enkephalin is expressed in both the scs and sbn and may function to modulate song qu ...201122198223
tetra-aqua-bis-(2-{[5-(pyridin-4-yl)-1,3,4-oxadiazol-2-yl]sulfan-yl}acetato)-cobalt(ii) monohydrate.in the title compound, [co(c(9)h(6)n(3)o(3)s)(2)(h(2)o)(4)]·h(2)o, the two 2-{[5-(pyridin-4-yl)-1,3,4-oxadiazol-2-yl]sulfan-yl}acetate ligands are monodentate. one coordinates the metal atom via the pyridyl n atom whereas the other coordinates via the carboxyl-ate o atom. the co(ii) atom adopts a slightly distorted octa-hedral coordination geometry with four o atoms of the coordinated water mol-ecules located in the equatorial plane and the n and o atoms of the two poa ligands in axial positions ...201122199545
preventive measures against sexually transmitted infections among female sex workers in lanzhou, china.background: we assessed the effects of preventive practices on sexually transmitted infections (stis) and reproductive tract infections (rtis) among female sex workers (fsws) in lanzhou, china. methods: a cross-sectional survey was conducted among 350 fsws from november 2008 to january 2009. demographic information and behavioural factors associated with stis were collected from all the participants. blood samples and cervical swabs were obtained, and genital and cervical examinations were done ...201122200110
inclusion of 30-day postdischarge detection triples the incidence of hospital-onset methicillin-resistant staphylococcus aureus.background. hospitalized patients are at increased risk for acquisition of methicillin-resistant staphylococcus aureus (mrsa). as hospital length of stay shortens, hospital-acquired mrsa events may be more likely to be detected after discharge. objective. we assessed the impact of attributing mrsa cases discovered within 30 days after discharge to the most recent hospitalization and identified patient characteristics associated with mrsa detection after discharge. design. retrospective cohort ...201222227979
immunocytochemical localization of kisspeptin neurons in the rat forebrain with special reference to sexual dimorphism and interaction with gnrh neurons.kisspeptin/metastin has been implicated as a critical regulator in luteinizing hormone (lh) secretion and the reproductive system mediating the effect of estrogen on gnrh neurons. in the present study we examined the sex differences in the effects of estrogen on kiss1/kisspeptin expression in the forebrain by using gonadectomized rats to assess the interaction of kisspeptin and gnrh neurons. kiss1/kisspeptin cell bodies were abundant in the rostral periventricular area of the third ventricle ( ...201222240892
distribution and chemical coding pattern of the cocaine- and amphetamine-regulated transcript (cart) immunoreactivity in the preoptic area of the pig.this study provides a detailed description of cocaine-and amphetamine-regulated transcript (cart) distribution and the co-localization pattern of cart and gonadotropin releasing hormone (gnrh), somatostatin (som), neuropeptide y (npy), cholecystokinin (cck), and substance p (sp) in the preoptic area (poa) of the domestic pig. the poa displays a low density of immunoreactive cells and rich immunoreactivity for cart in fibers. cart-immunoreactive (cart-ir) cell bodies were single and faintly s ...201122252754
socially modulated cell proliferation is independent of gonadal steroid hormones in the brain of the adult green treefrog (hyla cinerea).gonadal steroid hormones have been shown to influence adult neurogenesis in addition to their well-defined role in regulating social behavior. adult neurogenesis consists of several processes including cell proliferation, which can be studied via 5-bromo-2'-deoxyuridine (brdu) labeling. in a previous study we found that social stimulation altered both cell proliferation and levels of circulating gonadal steroids, leaving the issue of cause/effect unclear. in this study, we sought to determine wh ...201222269468
microscopic analysis of severe structural rearrangements of the plant endoplasmic reticulum and golgi caused by overexpression of poa semilatent virus movement protein.cell-to-cell transport of plant viruses is mediated by virus-encoded movement proteins and occurs through plasmodesmata interconnecting neighboring cells in plant tissues. three movement proteins coded by the "triple gene block" (tgb) and named tgbp1, tgbp2 and tgbp3 have distinct functions in viral transport. tgbp1 binds viral genomic rnas to form ribonucleoprotein complexes representing the transport form of viral genome, while tgbp2 and tgbp3 are necessary for intracellular delivery of such c ...201222272174
premature ovarian aging in primary infertility: triple x syndrome.genetic aberrations comprise one-third of women with premature ovarian aging (poa). x chromosome abnormalities are seen in these women. we report a case of a 29-year-old lady with primary infertility and poa. she was phenotypically normal and her basal follicle stimulating hormone level was above the age-specific cut-off. karyotype was triple x syndrome.201122346085
mating skew in barbary macaque males: the role of female mating synchrony, female behavior, and male-male coalitions.a fundamental question of sexual selection theory concerns the causes and consequences of reproductive skew among males. the priority of access (poa) model (altmann, ann ny acad sci 102:338-435, 1962) has been the most influential framework in primates living in permanent, mixed-sex groups, but to date it has only been tested with the appropriate data on female synchrony in a handful of species. in this paper, we used mating data from one large semi-free ranging group of barbary macaques: (1) to ...201122448085
warm sensitive neurons of the preoptic area regulate ambient temperature related changes in sleep in the rat.warm sensitive neurons (wsn) play a major role not only in body temperature regulation, but also in sleep regulation. the present study was undertaken to investigate the role of wsn of the preoptic area (poa) in mediating the ambient temperature (t(amb)) related changes in sleep. the effect of t(amb) changes on sleep and body temperature was studied in rats before and after destruction of wsn of the poa by local intracerebral injection of capsaicin. though the rats preferred 27 degrees c t(amb), ...201122471234
inhibition of acetylcholinesterase activity by rivastigmine decreases lipopolysaccharide-induced il-1β expression in the hypothalamus of ewes.the present study was designed to determine the effect of subcutaneous rivastigmine treatment on il-1β expression and il-1 type i receptor (il-1r1) gene expression in the hypothalamic structures (preoptic area [poa], anterior hypothalamus [aha], and medial basal hypothalamus [mbh]) of ewes after lipopolysaccharide (lps) treatment. endotoxin treatment increased (p ≤ 0.01) both il-1β and il-1r1 gene expression in the poa, aha, and mbh compared with the control group, whereas concomitant rivastigmi ...201223291013
surgical treatment versus expectant care in the management of incomplete miscarriage: a randomised controlled trial.to determine whether expectant care of incomplete miscarriages can significantly reduce the need for surgical evacuation of retained products of conception (erpc) without increasing complications.201223292054
hepatocellular carcinoma with blood supply from parasitized omental artery: angiographic appearance and chemoembolization.to analyze angiographic appearance of hepatocellular carcinoma (hcc) with blood supply from parasitized omental artery (poa), and evaluate the technical feasibility, safety and therapeutic efficacy of chemo-embolization via the poas.201223359452
fungal planet description sheets: 128-153.novel species of microfungi described in the present study include the following from australia: catenulostroma corymbiae from corymbia, devriesia stirlingiae from stirlingia, penidiella carpentariae from carpentaria, phaeococcomyces eucalypti from eucalyptus, phialophora livistonae from livistona, phyllosticta aristolochiicola from aristolochia, clitopilus austroprunulus on sclerophyll forest litter of eucalyptus regnans and toxicocladosporium posoqueriae from posoqueria. several species are al ...201223606771
the pseudomonas aeruginosa antimetabolite l-2-amino-4-methoxy-trans-3-butenoic acid inhibits growth of erwinia amylovora and acts as a seed germination-arrest factor.the pseudomonas aeruginosa antimetabolite l-2-amino-4-methoxy-trans-3-butenoic acid (amb) shares biological activities with 4-formylaminooxyvinylglycine, a related molecule produced by pseudomonas fluorescens wh6. we found that culture filtrates of a p. aeruginosa strain overproducing amb weakly interfered with seed germination of the grassy weed poa annua and strongly inhibited growth of erwinia amylovora, the causal agent of the devastating orchard crop disease known as fire blight. amb was ac ...201223757135
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