Publications

[product development on the basis of cereal and leguminous flours to coeliac disease in children aged 6-24 months; ii: properties of the mixtures].	the nutritional formulations of high protein content, provided by a flour mixture from two andean cultures, quinua (chenopodium quinua willd) and lupino (lupinus albus l), with two traditional cereals, maize (zea mays l.) and rice (oryza sativa l.), entailed to the preparation of a "sweet mixture" for the elaboration of "queques" and another "dessert mixture" flavoured with banana, that can be prepared with water or milk, constituted a good alternative as food supplement for the nutrition of chi ...	21519743
[product development on the basis of cereal and leguminous flours to coeliac disease in children between 6-24 months; i: formulation and acceptability].	the revaluation of the andean cultivations, quinua (chenopodium quinua willd) and lupin (lupinus albus l.), to be used in nutritional mixtures, with traditional cereals like corn (zea mays l.) and rice (oryza sativa l.), originate mixtures without gluten which constitute a good alternative for the nutrition of children under 24 months that suffer from celiac disease, since they improve the quality of the protein, by essential amino acids compensation, and also impacts in the product's diversific ...	21519742
control of abscisic acid catabolism and abscisic acid homeostasis is important for reproductive stage stress tolerance in cereals.	drought stress at the reproductive stage causes pollen sterility and grain loss in wheat (triticum aestivum). drought stress induces abscisic acid (aba) biosynthesis genes in anthers and aba accumulation in spikes of drought-sensitive wheat varieties. in contrast, drought-tolerant wheat accumulates lower aba levels, which correlates with lower aba biosynthesis and higher aba catabolic gene expression (aba 8'-hydroxylase). wheat taaba8'oh1 deletion lines accumulate higher spike aba levels and are ...	21502188
evident and latent plasticity across the rice diterpene synthase family with potential implications for the evolution of diterpenoid metabolism in the cereals.	the evolution of natural product biosynthetic pathways can be envisioned to occur via a number of mechanisms. in the present study we provide evidence that latent plasticity plays a role in such metabolic evolution. in particular, rice (oryza sativa) produces both ent- and syn-cpp (copalyl diphosphate), which are substrates for downstream diterpene synthases. in the present paper we report that several members of this enzymatic family exhibit dual reactivity with some pairing of ent-, syn- or no ...	21323642
septum formation in amyloplasts produces compound granules in the rice endosperm and is regulated by plastid division proteins.	storage tissues such as seed endosperm and tubers store starch in the form of granules in the amyloplast. in the rice (oryza sativa) endosperm, each amyloplast produces compound granules consisting of several dozen polyhedral, sharp-edged and easily separable granules; whereas in other cereals, including wheat (triticum aestivum), barley (hordeum vulgare) and maize (zea mays), each amyloplast synthesizes one granule. despite extensive studies on mutants of starch synthesis in cereals, the molecu ...	20685968
megabase level sequencing reveals contrasted organization and evolution patterns of the wheat gene and transposable element spaces.	to improve our understanding of the organization and evolution of the wheat (triticum aestivum) genome, we sequenced and annotated 13-mb contigs (18.2 mb) originating from different regions of its largest chromosome, 3b (1 gb), and produced a 2x chromosome survey by shotgun illumina/solexa sequencing. all regions carried genes irrespective of their chromosomal location. however, gene distribution was not random, with 75% of them clustered into small islands containing three genes on average. a t ...	20581307
an in planta, agrobacterium-mediated transient gene expression method for inducing gene silencing in rice (oryza sativa l.) leaves.	localized introduction and transient expression of t-dna constructs mediated by agro-infiltration of leaf tissues has been largely used in dicot plants for analyzing the transitivity and the cell-to cell movement of the rnai signal. in cereals, however, the morphology of the leaf and particularly the structure of the leaf epidermis, prevent infiltration of a bacterial suspension in cells by simple pressure, a method otherwise successful in dicots leaves. this study aimed at establishing a rapid ...	24279881
rice-induced anaphylaxis: ige-mediated allergy against a 56-kda glycoprotein.	although rice (oryza sativa) is one of the most common cereals produced and consumed around the world, there have been only a few reports on immediate hypersensitivity reactions after ingestion of rice. few clinical studies on rice allergy in asia have been reported concerning rhinitis, asthma and atopic dermatitis. in this case study, we identify allergens presumably responsible for anaphylaxis after ingestion of rice in a german patient.	22205234
development of a novel aluminum tolerance phenotyping platform used for comparisons of cereal aluminum tolerance and investigations into rice aluminum tolerance mechanisms.	the genetic and physiological mechanisms of aluminum (al) tolerance have been well studied in certain cereal crops, and al tolerance genes have been identified in sorghum (sorghum bicolor) and wheat (triticum aestivum). rice (oryza sativa) has been reported to be highly al tolerant; however, a direct comparison of rice and other cereals has not been reported, and the mechanisms of rice al tolerance are poorly understood. to facilitate al tolerance phenotyping in rice, a high-throughput imaging s ...	20538888
a highly conserved gene island of three genes on chromosome 3b of hexaploid wheat: diverse gene function and genomic structure maintained in a tightly linked block.	the complexity of the wheat genome has resulted from waves of retrotransposable element insertions. gene deletions and disruptions generated by the fast replacement of repetitive elements in wheat have resulted in disruption of colinearity at a micro (sub-megabase) level among the cereals. in view of genomic changes that are possible within a given time span, conservation of genes between species tends to imply an important functional or regional constraint that does not permit a change in genom ...	20507561
crop management techniques to enhance harvest index in rice.	a major challenge in rice (oryza sativa l.) production is to enhance water use efficiency (wue) and maintain or even increase grain yield. wue, if defined as the biomass accumulation over water consumed, may be fairly constant for a given species in given climate. wue can be enhanced by less irrigation. however, such enhancement is largely a trade-off against lower biomass production. if wue is defined as the grain production per unit amount of water irrigated, it would be possible to increase w ...	20421195
maize (zea mays): a model organism for basic and applied research in plant biology.	zea mays ssp. mays is one of the world's most important crop plants, boasting a multibillion dollar annual revenue. in addition to its agronomic importance, maize has been a keystone model organism for basic research for nearly a century. within the cereals, which include other plant model species such as rice (oryza sativa), sorghum (sorghum bicolor), wheat (triticum spp.), and barley (hordeum vulgare), maize is the most thoroughly researched genetic system. several attributes of the maize plan ...	20147033
nanosims analysis of arsenic and selenium in cereal grain.	cereals are an important source of selenium (se) to humans and many people have inadequate intakes of this essential trace element. conversely, arsenic (as) is toxic and may accumulate in rice grain at levels that pose a health risk. knowledge of the localization of selenium and arsenic within the cereal grain will aid understanding of their deposition patterns and the impact of processes such as milling. high-resolution secondary ion mass spectrometry (nanosims) was used to determine the loca ...	19895416
a transporter at the node responsible for intervascular transfer of silicon in rice.	the concentration of essential mineral nutrients in the edible portion of plants such as grains may affect the nutritional value of these foods, while concentrations of toxic minerals in the plant are matter of food safety. minerals taken up by the roots from soils are normally redirected at plant nodes before they are finally transported into developing seeds. however, the molecular mechanisms involved in this process have not been identified so far. herein, we report on a transporter (lsi6) re ...	19734433
targeted mapping of cdu1, a major locus regulating grain cadmium concentration in durum wheat (triticum turgidum l. var durum).	some durum wheat (triticum turgidum l. var durum) cultivars have the genetic propensity to accumulate cadmium (cd) in the grain. a major gene controlling grain cd concentration designated as cdu1 has been reported on 5b, but the genetic factor(s) conferring the low cd phenotype are currently unknown. the objectives of this study were to saturate the chromosomal region harboring cdu1 with newly developed pcr-based markers and to investigate the colinearity of this wheat chromosomal region with ri ...	20559817
fine mapping of a grain-weight quantitative trait locus in the pericentromeric region of rice chromosome 3.	as the basis for fine mapping of a grain-weight qtl, gw3.1, a set of near isogenic lines (nils), was developed from an oryza sativa, cv. jefferson x o. rufipogon (irgc105491) population based on five generations of backcrossing and seven generations of selfing. despite the use of an interspecific cross for mapping and the pericentromeric location of the qtl, we observed no suppression of recombination and have been able to narrow down the location of the gene underlying this qtl to a 93.8-kb reg ...	15611185
induction of cytoplasmic mannose-binding jacalin-related lectins is a common phenomenon in cereals treated with jasmonate methyl ester.	treatment of whole plants with jasmonic acid methyl ester induces lectin activity in leaves of oryza sativa, hordeum vulgare, triticum vulgare, secale cereale and zea mays. purification and characterization of the lectins revealed that they all have a very similar molecular structure and carbohydrate-binding properties. further analysis of the cdna clones encoding the lectins revealed that they all belong to the family of cytoplasmic mannose-specific jacalin-related lectins.	15560260
comparative sequence analysis of the region harboring the hardness locus in barley and its colinear region in rice.	the ancestral shared synteny concept has been advocated as an approach to positionally clone genes from complex genomes. however, the unified grass genome model and the study of grasses as a single syntenic genome is a topic of considerable controversy. hence, more quantitative studies of cereal colinearity at the sequence level are required. this study compared a contiguous 300-kb sequence of the barley (hordeum vulgare) genome with the colinear region in rice (oryza sativa). the barley sequenc ...	15466237
allele mining for stress tolerance genes in oryza species and related germplasm.	allele mining exploits the deoxyribonucleic acid (dna) sequence of one genotype to isolate useful alleles from related genotypes. the international project to sequence the genome of oryza sativa l cv. nipponbare will make allele mining possible for all genes of rice and possibly related cereals. we used a rice calmodulin gene, a rice gene encoding a late embryogenesis-associated protein, and salt-inducible rice gene to optimize the polymerase chain reaction (pcr) for allele mining of stress tole ...	15208452
heterogeneous expression patterns and separate roles of the sepallata gene leafy hull sterile1 in grasses.	sepallata (sep) genes exhibit distinct patterns of expression and function in the grass species rice (oryza sativa) and maize (zea mays), suggesting that the role of the genes has changed during the evolution of the family. here, we examine expression of the sep-like gene leafy hull sterile1 (lhs1) in phylogenetically disparate grasses, reconstruct the pattern of gene expression evolution within the family, and then use the expression patterns to test hypotheses of gene function. our data suppor ...	15208396
from weeds to crops: genetic analysis of root development in cereals.	root development of arabidopsis, zea mays (maize) and oryza sativa (rice) differs in both overall architecture and the anatomy of individual roots. in maize and rice, the post-embryonic shoot-borne root system becomes the major backbone of the root stock; in arabidopsis, the embryonic root system formed by a simple primary root and its lateral roots remains dominant. recently, several specific root mutants and root-specific genes have been identified and characterized in maize and rice. interest ...	14729218
adventitious root formation in rice requires osgnom1 and is mediated by the ospins family.	the fibrous root system in cereals comprises primarily adventitious roots (ars), which play important roles in nutrient and water uptake. current knowledge regarding the molecular mechanism underlying ar development is still limited. we report here the isolation of four rice (oryza sativa l.) mutants, from different genetic backgrounds, all of which were defective in ar formation. these mutants exhibited reduced numbers of lateral roots (lrs) and partial loss of gravitropism. the mutants also di ...	19546891
the c-glycosylation of flavonoids in cereals.	flavonoids normally accumulate in plants as o-glycosylated derivatives, but several species, including major cereal crops, predominantly synthesize flavone-c-glycosides, which are stable to hydrolysis and are biologically active both in planta and as dietary components. an enzyme (oscgt) catalyzing the udp-glucose-dependent c-glucosylation of 2-hydroxyflavanone precursors of flavonoids has been identified and cloned from rice (oryza sativa ssp. indica), with a similar protein characterized in wh ...	19411659
evolutionary and expression study of the aldehyde dehydrogenase (aldh) gene superfamily in rice (oryza sativa).	aldehyde dehydrogenase (aldh) superfamily represents a group of nad(p)(+)-dependent enzymes that catalyze the oxidation of endogenous and exogenous aldehydes to the corresponding carboxylic acids. a total of twenty aldh genes were identified in the rice genome. they were grouped into 10 distinct families based on protein sequence identity. the whole genome duplication (wgd) predating the divergence of cereals and tandem duplications represent the major mechanism for this superfamily expansion. i ...	19071198
quantitative and qualitative studies of silica in different rice samples grown in north of iran using uv-vis, xrd and ir spectroscopy techniques.	silicon is an essential trace element and is found in vegetables, fruits, cereals, water, pasta and rice (oryza sativa). in this work, the silica content of different types of rice grains were measured. here, we used the heteropoly blue photometric method with a double beam uv-vis spectrophotometer to determine the amount of silicon in rice samples (n=7) that were collected in the north of iran. the samples were digested with wet-ashing method by microwave-assisted heating and then treated with ...	18970836
mutations of genes in synthesis of the carotenoid precursors of aba lead to pre-harvest sprouting and photo-oxidation in rice.	pre-harvest sprouting (phs) or vivipary in cereals is an important agronomic trait that results in significant economic loss. a considerable number of mutations that cause phs have been identified in several species. however, relatively few viviparous mutants in rice (oryza sativa l.) have been reported. to explore the mechanism of phs in rice, we carried out an extensive genetic screening and identified 12 phs mutants (phs). based on their phenotypes, these phs mutants were classified into thre ...	18208525
alpha-amylase production is induced by sulfuric acid in rice aleurone cells.	the hydrolytic enzyme alpha-amylase (ec 3.2.1.1) is produced mainly in aleurone cells of germinating cereals, and the phytohormone gibberellin (ga) is essential for its induction. however, in rice (oryza sativa l.), sulfuric acid (h(2)so(4)) induces alpha-amylase production in aleurone tissue even in the absence of ga. here, the pre-treatment of rice aleurone cells with h(2)so(4) and incubation in water induced alpha-amylase activity, as if the cells had been incubated in ga solution.	17988885
conservation and divergence of apetala1/fruitfull-like gene function in grasses: evidence from gene expression analyses.	duplicated apetala1/fruitfull (ap1/ful) genes show distinct but overlapping patterns of expression within rice (oryza sativa) and within ryegrass (lolium temulentum), suggesting discrete functional roles in the transition to flowering, specification of spikelet meristem identity, and specification of floral organ identity. in this study, we analyzed the expression of the ap1/ful paralogues ful1 and ful2 across phylogenetically disparate grasses to test hypotheses of gene function. in combination ...	17666026
gene targeting by homologous recombination as a biotechnological tool for rice functional genomics.	the modification of an endogenous gene into a designed sequence by homologous recombination, termed gene targeting (gt), has broad implications for basic and applied research. rice (oryza sativa), with a sequenced genome of 389 mb, is one of the most important crops and a model plant for cereals, and the single-copy gene waxy on chromosome 6 has been modified with a frequency of 1% per surviving callus by gt using a strong positive-negative selection. because the strategy is independent of gene- ...	17449652
putative fasciclin-like arabinogalactan-proteins (fla) in wheat (triticum aestivum) and rice (oryza sativa): identification and bioinformatic analyses.	putative plant adhesion molecules include arabinogalactan-proteins having fasciclin-like domains. in animal, fasciclin proteins participate in cell adhesion and communication. however, the molecular basis of interactions in plants is still unknown and none of these domains have been characterized in cereals. this work reports the characterization of 34 wheat (triticum aestivum) and 24 rice (oryza sativa) fasciclin-like arabinogalactan-proteins (flas). bioinformatics analyses show that cereal fla ...	16944204
computational prediction and experimental verification of hva1-like abscisic acid responsive promoters in rice (oryza sativa).	abscisic acid (aba) is one of the central plant hormones, responsible for controlling both maturation and germination in seeds, as well as mediating adaptive responses to desiccation, injury, and pathogen infection in vegetative tissues. thorough analyses of two barley genes, hva1 and hva22, indicate that their response to aba relies on the interaction of two cis-acting elements in their promoters, an aba response element (abre) and a coupling element (ce). together, they form an aba response pr ...	16845480
rapid evolution in sequence and length of the nuclear-located gene for mitochondrial l2 ribosomal protein in cereals.	the l2 ribosomal protein is typically one of the most conserved proteins in the ribosome and is universally present in bacterial, archaeal, and eukaryotic cytosolic and organellar ribosomes. it is usually 260-270 amino acids long and its binding to the large-subunit ribosomal rna near the peptidyl transferase center is mediated by a beta-barrel rna-binding domain with 10 beta strands. in the diverse land plants marchantia polymorpha (liverwort) and oryza sativa (rice), the mitochondrial-encoded ...	16604111
posttranslational regulation of pyruvate, orthophosphate dikinase in developing rice (oryza sativa) seeds.	pyruvate, orthophosphate dikinase (ppdk; e.c.2.7.9.1) is most well known as a photosynthetic enzyme in c4 plants. the enzyme is also ubiquitous in c3 plant tissues, although a precise non-photosynthetic c3 function(s) is yet to be validated, owing largely to its low abundance in most c3 organs. the single c3 organ type where ppdk is in high abundance, and, therefore, where its function is most amenable to elucidation, are the developing seeds of graminaceous cereals. in this report, we suggest a ...	16596412
grain filling of cereals under soil drying.	monocarpic plants require the initiation of whole-plant senescence to remobilize and transfer assimilates pre-stored in vegetative tissues to grains. delayed whole-plant senescence caused by either heavy use of nitrogen fertilizer or adoption of lodging-resistant cultivars/hybrids that remain green when the grains are due to ripen results in a low harvest index with much nonstructural carbohydrate (nsc) left in the straw. usually, water stress during the grain-filling period induces early senesc ...	16411926
high transferability of bread wheat est-derived ssrs to other cereals.	the increasing availability of expressed sequence tags (ests) in wheat (triticum aestivum) and related cereals provides a valuable resource of non-anonymous dna molecular markers. in this study, 300 primer pairs were designed from 265 wheat ests that contain microsatellites in order to develop new markers for wheat. their level of transferability in eight related species [triticum durum, t. monococcum, aegilops speltoides, ae. tauschii, rye (secale cereale), barley (hordeum vulgare), agropyron e ...	16034582
arl1, a lob-domain protein required for adventitious root formation in rice.	adventitious roots constitute the bulk of the fibrous root system in cereals. compared with the current understanding of shoot development, knowledge of the molecular mechanisms of development of the adventitious roots of cereals is limited. we have isolated and characterized a novel gene controlling the initiation of adventitious root primordia in rice (oryza sativa l.). the gene, designated adventitious rootless1 (arl1), encodes a protein with a lateral organ boundaries (lob) domain. it is exp ...	15960615
nonrandom distribution and frequencies of genomic and est-derived microsatellite markers in rice, wheat, and barley.	earlier comparative maps between the genomes of rice (oryza sativa l.), barley (hordeum vulgare l.) and wheat (triticum aestivum l.) were linkage maps based on cdna-rflp markers. the low number of polymorphic rflp markers has limited the development of dense genetic maps in wheat and the number of available anchor points in comparative maps. higher density comparative maps using pcr-based anchor markers are necessary to better estimate the conservation of colinearity among cereal genomes. the pu ...	15720707
structural and histochemical studies on grain-filling in the caryopsis of rice (oryza sativa l.).	the endosperm and embryo that constitute the filial tissues of rice caryopsis are isolated from the maternal tissues by the absence of any symplastic continuity. nutrients are transported to the endosperm through a single ovular vascular trace present on the ventral side of the ovary. initially solute enters through the chalaza into the nucellar projection and then into the endosperm. at later stages transport occurs through the nucellar epidermis, centripetally towards the endosperm. the cell w ...	12799492
targeting the aluminum tolerance gene alt3 region in rye, using rice/rye micro-colinearity.	characterization and manipulation of aluminum (al) tolerance genes offers a solution to al toxicity problems in crop cultivation on acid soil, which composes approximately 40% of all arable land. by exploiting the rice (oryza sativa l.)/rye (secale cereale l.) syntenic relationship, the potential for map-based cloning of genes controlling al tolerance in rye (the most al-tolerant cereal) was explored. an attempt to clone an al tolerance gene (alt3) from rye was initiated by using dna markers fla ...	15688201
two ancient rounds of polyploidy in rice genome.	an ancient genome duplication (ppp1) that predates divergence of the cereals has recently been recognized. we report here another potentially older large-scale duplication (ppp2) event that predates monocot-dicot divergence in the genome of rice (oryza sativa l.), as inferred from the age distribution of pairs of duplicate genes based on recent genome data for rice. our results suggest that paleopolyploidy was widespread and played an important role in the evolution of rice.	15633242
the evolution of constans-like gene families in barley, rice, and arabidopsis.	the co (constans) gene of arabidopsis has an important role in the regulation of flowering by photoperiod. co is part of a gene family with 17 members that are subdivided into three classes, termed group i to iii here. all members of the family have a cct (co, co-like, toc1) domain near the carboxy terminus. group i genes, which include co, have two zinc finger b-boxes near the amino terminus. group ii genes have one b-box, and group iii genes have one b-box and a second diverged zinc finger. an ...	12692345
convergent evolution of perenniality in rice and sorghum.	annual and perennial habit are two major strategies by which grasses adapt to seasonal environmental change, and these distinguish cultivated cereals from their wild relatives. rhizomatousness, a key trait contributing to perenniality, was investigated by using an f(2) population from a cross between cultivated rice (oryza sativa) and its wild relative, oryza longistaminata. molecular mapping based on a complete simple sequence-repeat map revealed two dominant-complementary genes controlling rhi ...	12642667
sugar modulation of alpha-amylase genes under anoxia.	tolerance to low oxygen availability is likely to be due to the interaction of several factors. sugar availability is one of the elements required to support anaerobic metabolism. in cereal grains the availability of soluble sugars is limited, while starch is stored in large amounts. degradation of starch under anoxia is therefore needed to avoid sugar starvation leading to rapid cell death. the striking difference in the ability to produce alpha-amylase when comparing the anoxia-tolerant rice ( ...	12509335
mapping of genes controlling aluminum tolerance in rice: comparison of different genetic backgrounds.	aluminum toxicity is the main factor limiting the productivity of crop plants in acid soils, particularly in the tropics and subtropics. in this study, a doubled-haploid population derived from the rice ( oryza sativa l.) breeding lines ct9993 and ir62266 was used to map genes controlling al tolerance. a genetic linkage map consisting of 280 dna markers (rflp, aflp and ssr) was constructed to determine the position and nature of quantitative trait loci (qtls) affecting al tolerance. three charac ...	12207224
barley cbf3 gene identification, expression pattern, and map location.	although cold and drought adaptation in cereals and other plants involve the induction of a large number of genes, inheritance studies in triticeae (wheat [triticum aestivum], barley [hordeum vulgare], and rye [secale cereale]) have revealed only a few major loci for frost or drought tolerance that are consistent across multiple genetic backgrounds and environments. one might imagine that these loci could encode highly conserved regulatory factors that have global effects on gene expression; the ...	12177491