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tracking the genome-wide outcomes of a transposable element burst over decades of amplification.to understand the success strategies of transposable elements (tes) that attain high copy numbers, we analyzed two pairs of rice (oryza sativa) strains, eg4/heg4 and a119/a123, undergoing decades of rapid amplification (bursts) of the class 2 autonomous ping element and the nonautonomous miniature inverted repeat transposable element (mite) mping comparative analyses of whole-genome sequences of the two strain pairs validated that each pair has been maintained for decades as inbreds since diverg ...201729158416
mapping quantitative trait loci for heat tolerance at the booting stage using chromosomal segment substitution lines in rice.high temperature stress is a major obstacle in rice productivity. considerable progress has been made on studying heat tolerance (ht) at different stages. however, the genetic basis of ht at the booting stage is poorly understood. in this study, we analyzed the morphological features of a heat-sensitive japonica cultivar sasanishiki under natural high temperature stress at the booting stage. the anthers became smaller and the number, and fertility, of pollen grains were decreased significantly. ...201729158631
gene expression and activity of antioxidant enzymes in rice plants, cv. brs ag, under saline stress.the rice cultivar (oryza sativa l.) brs ag, developed by embrapa clima temperado, is the first cultivar designed for purposes other than human consumption. it may be used in ethanol production and animal feed. different abiotic stresses negatively affect plant growth. soil salinity is responsible for a serious reduction in productivity. therefore, the objective of this study was to evaluate the gene expression and the activity of antioxidant enzymes (sod, cat, apx and gr) and identify their func ...201729158635
genome-wide in silico identification of membrane-bound transcription factors in plant species.membrane-bound transcription factors (mtfs) are located in cellular membranes due to their transmembrane domains. in plants, proteolytic processing is considered to be the main mechanism for mtf activation, which ensures the liberation of mtfs from membranes and further their translocation into the nucleus to regulate gene expression; this process skips both the transcriptional and translational stages, and thus it guarantees the prompt responses of plants to various stimuli. currently, informat ...201729158982
sugar flux and signaling in plant-microbe interactions.plant breeders have developed crop plants that are resistant to pests, but the continual evolution of pathogens creates the need to iteratively develop new control strategies. molecular tools have allowed us to gain deep insights into disease responses, allowing for more efficient, rational engineering of crops that are more robust or resistant to a greater number of pathogen variants. here we describe the roles of sweet and stp transporters, membrane proteins that mediate transport of sugars ac ...201729160592
a hypersensitivity-like response to meloidogyne graminicola in rice (oryza sativa l.).meloidogyne graminicola (m. graminicola) is a major plant-parasitic nematode affecting rice cultivation in asia. resistance to this nematode was found in the african rice genotypes oryza glaberrima and o. longistaminata, however, due to interspecific hybrid sterility, the introgression of resistance genes in the widely consumed o. sativa varieties remains challenging. recently, resistance was found in o. sativa and here we report for the first time the histological and genetic characterization o ...201729161206
suppression of microrna159 impacts multiple agronomic traits in rice (oryza sativa l.).micrornas (mirnas) are important regulators in plant growth and development. mir159 is a conserved mirna among different plant species and has various functions in plants. studies on mir159 are mostly done on model plant, arabidopsis thaliana. in rice, studies on mir159 were either based upon genome-wide expression analyses focused upon responses to different nitrogen forms and abiotic stress or upon phenotypic studies of transgenic plants overexpressing its precursor. sttm (short tandem target ...201729162059
rice tsv3 encoding obg-like gtpase protein is essential for chloroplast development during the early leaf stage under cold stress.the spo0b-associated gtp-binding (obg) proteins are essential for the viability of nearly all bacteria. however, the detailed roles of obg proteins in higher plants have not yet been elucidated. in this study, we identified a novel rice (oryza sativa l.) thermo-sensitive virescent mutant (tsv3) that displayed an albino phenotype at 20° before the three-leaf stage while being a normal green at 32° or even at 20° after the four-leaf stage. the mutant phenotype was consistent with altered chlorophy ...201829162684
silencing of osgrxs17 in rice improves drought stress tolerance by modulating ros accumulation and stomatal closure.glutaredoxins (grxs) modulate redox-dependent signaling pathways and have emerged as key mediators in plant responses to environmental stimuli. here we report that rnai-mediated suppression of oryza sativa grxs17 (osgrxs17) improved drought tolerance in rice. gene expression studies showed that osgrxs17 was present throughout the plant and that transcript abundance increased in response to drought stress and abscisic acid (aba) treatment. localization studies, utilizing gfp-osgrxs17 fusion prote ...201729162892
partial loss-of-function of nal1 alters canopy photosynthesis by changing the contribution of upper and lower canopy leaves in rice.little is known about the genetic basis of leaf and canopy photosynthesis. here we aimed to detect novel quantitative trait loci (qtl) controlling photosynthesis by increasing leaf nitrogen content (lnc) per leaf area and analysed its effect on leaf and canopy photosynthesis. to identify qtl that increase photosynthetic rate in leaves, we screened chromosome segment substitution lines (cssls) of oryza sativa ssp. japonica cultivar koshihikari and o. sativa ssp. indica cultivar nona bokra using l ...201729162918
genetic dissection of the major quantitative trait locus (qse11), and its validation as the major influence on the rate of stigma exsertion in rice (oryza sativa l.).the rate of stigma exsertion (se) is an important trait in rice breeding because the efficiency of hybrid rice seed production can be improved by increasing the percentage of stigmas that exsert. in this study, we developed a near isogenic line (nil) from two parents, xieqingzaob (xqzb) and zhonghoi9308 (zh9308), which have high and low se rates in that order. in our previous study, we employed 75 chromosome segment substitution lines (cssls) and analyzed quantitative trait loci (qtls) for their ...201729163563
canopy chlorophyll density based index for estimating nitrogen status and predicting grain yield in rice.canopy chlorophyll density (chl) has a pivotal role in diagnosing crop growth and nutrition status. the purpose of this study was to develop chl based models for estimating n status and predicting grain yield of rice (oryza sativa l.) with leaf area index (lai) and chlorophyll concentration of the upper leaves. six field experiments were conducted in jiangsu province of east china during 2007, 2008, 2009, 2013, and 2014. different n rates were applied to generate contrasting conditions of n avai ...201729163568
osarm1, an r2r3 myb transcription factor, is involved in regulation of the response to arsenic stress in rice.bioaccumulation of arsenic (as) in rice (oryza sativa) increases human exposure to this toxic, carcinogenic element. recent studies identified several as transporters, but the regulation of these transporters remains unclear. here, we show that the rice r2r3 myb transcription factor osarm1 (arsenite-responsive myb1) regulates as-associated transporters genes. treatment with as(iii) induced osarm1 transcript accumulation and an osarm1-gfp fusion localized to the nucleus. histochemical analysis of ...201729163593
increasing leaf vein density via mutagenesis in rice results in an enhanced rate of photosynthesis, smaller cell sizes and can reduce interveinal mesophyll cell number.improvements to leaf photosynthetic rates of crops can be achieved by targeted manipulation of individual component processes, such as the activity and properties of rubisco or photoprotection. this study shows that simple forward genetic screens of mutant populations can also be used to rapidly generate photosynthesis variants that are useful for breeding. increasing leaf vein density (concentration of vascular tissue per unit leaf area) has important implications for plant hydraulic properties ...201729163607
oshak1, a high-affinity potassium transporter, positively regulates responses to drought stress in rice.drought is one of the environmental factors that severely restrict plant distribution and crop production. recently, we reported that the high-affinity potassium transporter oshak1 plays important roles in k acquisition and translocation in rice over low and high k concentration ranges, however, knowledge on the regulatory roles of oshak1 in osmotic/drought stress is limited. here, transcript levels of oshak1 were found transiently elevated by water deficit in roots and shoots, consistent with t ...201729163608
analysis of bronopol (2-bromo-2-nitropropan-1, 3-diol) residues in rice (oryza sativa l.) by spe using bond elut plexa and liquid chromatography-tandem mass spectrometry.a novel method has been developed for the direct, sensitive, and rapid detection of bronopol in rice using a simple solid-phase extraction (spe) procedure followed by liquid chromatography-tandem mass spectrometry (lc-ms/ms), with electrospray ionization (esi). bronopol was stable under acidic conditions, and an acidic environment was thus needed before sample loading to ensure the stability of bronopol. rice extracts containing bronopol were pretreated using a hydrophilic-lipophilic balanced (b ...201829164282
drying-submergence alternation enhanced crystalline ratio and varied surface properties of iron plaque on rice (oryza sativa) roots.iron plaque (ip) is valuable in nutrient management and contaminant tolerance for rice (oryza sativa) because it can adsorb various nutrients and toxic ions. crystalline ratio (cr) can be defined as the proportion of crystalline iron (ci) to total ip to describe ip crystallinity. although the knowledge on ip has abounded, the information on the relationship among its formation condition, surface properties, and cr remains insufficient. in this study, quartz sand-soil cultivation with rice was co ...201729164457
funcoup 4: new species, data, and visualization.this release of the funcoup database (http://funcoup.sbc.su.se) is the fourth generation of one of the most comprehensive databases for genome-wide functional association networks. these functional associations are inferred via integrating various data types using a naive bayesian algorithm and orthology based information transfer across different species. this approach provides high coverage of the included genomes as well as high quality of inferred interactions. in this update of funcoup we i ...201829165593
effect of copper on the translocation and transformation of polychlorinated biphenyls in rice.contamination of organic pollutants in the environment is usually accompanied by heavy metals. however, a little information on the influences of heavy metals on the uptake, translocation and transformation of organic pollutants in plants is available. in this study, ten-day hydroponic exposure was conducted to explore the influence of copper (cu) on the bioaccumulation and biotransformation of polychlorinated biphenyls (pcbs) in intact young rice (oryza sativa l.). low dose of cu (≤100 μmol/l) ...201829169126
can liming reduce cadmium (cd) accumulation in rice (oryza sativa) in slightly acidic soils? a contradictory dynamic equilibrium between cd uptake capacity of roots and cd immobilisation in soils.cadmium (cd) accumulation in rice is strongly controlled by liming, but information on the use of liming to control cd accumulation in rice grown in slightly acidic soils is inconsistent. here, pot experiments were carried out to investigate the mechanisms of liming on cd accumulation in two rice varieties focusing on two aspects: available/exchangeable cd content in soils that were highly responsive to liming, and cd uptake and transport capacity in the roots of rice in terms of cd accumulation ...201829169130
dwarf14, a receptor covalently linked with the active form of strigolactones, undergoes strigolactone-dependent degradation in rice.strigolactones (sls) are the latest confirmed phytohormones that regulate shoot branching by inhibiting bud outgrowth in higher plants. perception of sls depends on a novel mechanism employing an enzyme-receptor dwarf14 (d14) that hydrolyzes sls and becomes covalently modified. this stimulates the interaction between d14 and d3, leading to the ubiquitination and degradation of the transcriptional repressor protein d53. however, the regulation of sl perception in rice remains elusive. in this stu ...201729170677
characterization of an acetohydroxy acid synthase mutant conferring tolerance to imidazolinone herbicides in rice (oryza sativa).the acetohydroxy acid synthase s627n mutation confers herbicide tolerance in rice, and the rice variety containing this mutation produces good yields. this variety is commercially viable at shanghai and jiangsu regions in china. weedy rice is a type of rice that produces lower yields and poorer quality grains than cultivated rice. it plagues commercial rice fields in many countries. one strategy to control its proliferation is to develop rice varieties that are tolerant to specific herbicides. a ...201729170911
plant-type phytoene desaturase: functional evaluation of structural implications.phytoene desaturase (pds) is an essential plant carotenoid biosynthetic enzyme and a prominent target of certain inhibitors, such as norflurazon, acting as bleaching herbicides. pds catalyzes the introduction of two double bonds into 15-cis-phytoene, yielding 9,15,9'-tri-cis-ζ-carotene via the intermediate 9,15-di-cis-phytofluene. we present the necessary data to scrutinize functional implications inferred from the recently resolved crystal structure of oryza sativa pds in a complex with norflur ...201729176862
open glume1: a key enzyme reducing the precursor of ja, participates in carbohydrate transport of lodicules during anthesis in rice.og1 is involved in ja-regulated anthesis by modulating carbohydrate transport of lodicules in rice. flowering plants have evolved a sophisticated regulatory network to coordinate anthesis and maximize reproductive success. in addition to various environmental conditions, the plant hormone jasmonic acid and its derivatives (jas) are involved in anthesis. however, the underlying mechanism remains largely unexplored. here, we report a ja-defective mutant in rice (oryza sativa), namely open glume 1, ...201829177846
a modified gc-specific maker gene annotation method reveals improved and novel gene predictions of high and low gc content in oryza sativa.accurate structural annotation depends on well-trained gene prediction programs. training data for gene prediction programs are often chosen randomly from a subset of high-quality genes that ideally represent the variation found within a genome. one aspect of gene variation is gc content, which differs across species and is bimodal in grass genomes. when gene prediction programs are trained on a subset of grass genes with random gc content, they are effectively being trained on two classes of ge ...201729178822
osmir396d affects gibberellin and brassinosteroid signaling to regulate plant architecture in rice.genetic improvement of plant architecture is one of the strategies for increasing the yield potential of rice (oryza sativa). although great progress has been made in the understanding of plant architecture regulation, the precise mechanism is still an urgent need to be revealed. here, we report that over-expression of osmir396d in rice results in semidwarf and increased leaf angle, a typical phenotype of brassinosteroid (br) enhanced mutant. osmir396d is involved in the interaction network of b ...201829180380
identification and characterization of the diverse stress-responsive r2r3-rmyb transcription factor from hibiscus sabdariffa l.various regulatory proteins play a fundamental role to manage the healthy plant growth under stress conditions. differential display reverse transcriptase pcr and random amplification of cdna ends (race) was used to explore the osmotic stress-responsive transcripts. we identified and characterized the salt stress-responsive r2r3 type rmyb transcription factor from hibiscus sabdariffa which has an open reading frame of 690 bp, encoding 229 long chain amino acids. in silico analysis confirmed the ...201729181384
resistance in 27 rice cultivars to sugarcane borer (lepidoptera: crambidae).rice (oryza sativa l.) is considered one of the most important crops in the world, and the sugarcane borer, diatraea saccharalis fabricius (lepidoptera: crambidae), is among the key pests damaging the crop in the americas. the objective of this work was to identify rice genotypes as a source of resistance to d. saccharalis. rice plants were infested in the greenhouse and subsequently evaluated for damage, larval weight and survival, and stem size. the cultivars 'bonança', 'caripuna', 'ir 42', 'c ...201729182781
alr encoding dcmp deaminase is critical for dna damage repair, cell cycle progression and plant development in rice.deoxycytidine monophosphate deaminase (dcmp deaminase, dcd) is crucial to the production of dttp needed for dna replication and damage repair. however, the effect of dcd deficiency and its molecular mechanism are poorly understood in plants. here, we isolated and characterized a rice albinic leaf and growth retardation (alr) mutant that is manifested by albinic leaves, dwarf stature and necrotic lesions. map-based cloning and complementation revealed that alr encodes a dcd protein. osdcd was exp ...201729186482
differential transcriptome profiling of chilling stress response between shoots and rhizomes of oryza longistaminata using rna sequencing.rice (oryza sativa) is very sensitive to chilling stress at seedling and reproductive stages, whereas wild rice, o. longistaminata, tolerates non-freezing cold temperatures and has overwintering ability. elucidating the molecular mechanisms of chilling tolerance (ct) in o. longistaminata should thus provide a basis for rice ct improvement through molecular breeding. in this study, high-throughput rna sequencing was performed to profile global transcriptome alterations and crucial genes involved ...201729190752
indel marker based genetic differentiation and genetic diversity in traditional rice (oryza sativa l.) landraces of chhattisgarh, india.rice has been cultivating and utilizing by humans for thousands of years under diverse environmental conditions. therefore, tremendous genetic differentiation and diversity has occurred at various agro-ecosystems. the significant indica-japonica differentiation in rice provides great opportunities for its genetic improvement. in the present investigation, a total of 42 polymorphic indel markers were used for differentiating 188 rice landraces and two local varieties of chhattisgarh, india into i ...201729190790
whole genome sequencing of oryza sativa l. cv. seeragasamba identifies a new fragrance allele in rice.fragrance of rice is an important trait that confers a large economic benefit to the farmers who cultivate aromatic rice varieties. several aromatic rice varieties have limited geographic distribution, and are endowed with variety-specific unique fragrances. badh2 was identified as a fragrance gene in 2005, and it is essential to identify the fragrance alleles from diverse geographical locations and genetic backgrounds. seeragasamba is a short-grain aromatic rice variety of the indica type, whic ...201729190814
genome-wide association study of rice grain width variation.seed size is variable within many plant species, and understanding the underlying genetic factors can provide insights into mechanisms of local environmental adaptation. here we make use of the abundant genomic and germplasm resources available for rice (oryza sativa) to perform a large-scale genome-wide association study (gwas) of grain width. grain width varies widely within the crop and is also known to show climate-associated variation across populations of its wild progenitor. using a filte ...201729193996
formation of protein disulfide bonds catalyzed by ospdil1;1 is mediated by microrna5144-3p in rice.correct folding of proteins in the endoplasmic reticulum is important for their stability and function under stress. protein disulfide isomerase (pdis) ospdil1;1 is a key protein-folding catalyst in rice (oryza sativa l.). here, microrna5144 (osa-mir5144-3p) is reported to mediate the formation of protein disulfide bonds via targeting ospdil1;1 mrna in rice seeds and seedlings during development and under conditions of abiotic stress, respectively. expression analysis of transgenic rice and iden ...201729194535
oscerk1 plays a crucial role in the lipopolysaccharide-induced immune response of rice.plant cell surface receptor-like kinases (rlks) mediate the signals from microbe-associated molecular patterns (mamps) that induce immune responses. lipopolysaccharide (lps), the major constituent of the outer membrane of gram-negative bacteria, is a common mamp perceived by animals and plants; however, the plant receptors/co-receptors are unknown except for lore, a bulb-type lectin s-domain rlk (b-lectin sd1-rlk) in arabidopsis. oscerk1 is a multifunctional rlk in rice that contains lysin motif ...201829194635
independent signalling cues underpin arbuscular mycorrhizal symbiosis and large lateral root induction in rice.perception of arbuscular mycorrhizal fungi (amf) triggers distinct plant signalling responses for parallel establishment of symbiosis and induction of lateral root formation. rice receptor kinase chitin elicitor receptor kinase 1 (cerk1) and α/β-fold hydrolase dwarf14-like (d14l) are involved in pre-symbiotic fungal perception. after 6 wk post-inoculation with rhizophagus irregularis, root developmental responses, fungal colonization and transcriptional responses were monitored in two independen ...201829194644
can rice (oryza sativa) mitigate pesticides and nutrients in agricultural runoff?phytoremediation of nutrients and pesticides in runoff is a growing conservation effort, particularly in agriculturally intensive areas such as the lower mississippi river valley. in the current study, rice (oryza sativa) was examined for its mitigation capacity of nitrogen, phosphorus, diazinon, and permethrin. twenty-two high density polyethylene circular containers (56 cm x 45 cm) were used as mesocosms, with 12 mesocosms planted with rice and 10 mesocosms remaining unvegetated. mesocosms wer ...201829196795
diversion of the long-chain acyl-acp pool in synechocystis to fatty alcohols through crispri repression of the essential phosphate acyltransferase plsx.fatty alcohol production in synechocystis sp. pcc 6803 was achieved through heterologous expression of the fatty acyl-coa/acp reductase maqu2220 from the bacteria marinobacter aquaeolei vt8 and the fatty acyl-acp reductase dpw from the rice oryza sativa. these platform strains became models for testing multiplex crispr-interference (crispri) metabolic engineering strategies to both improve fatty alcohol production and to study membrane homeostasis. crispri allowed partial repression of up to six ...201729199103
relationship of ros accumulation and superoxide dismutase isozymes in developing anther with floret fertility of rice under heat stress.high temperature (ht) at meiosis stage is one of most important environment constraint affecting spikelet fertility and rice yield. in this paper, the effects of ht exposure at meiosis stage on the ros (reactive oxygen species) accumulation, various superoxide dismutase (sod, ec1.15.1.11) isozymes in developing anther, and its relationship with ht-induced decline in pollen viability and floret fertility were investigated by using four rice cultivars differing in heat tolerance under well-control ...201829202329
identification of early ammonium nitrate-responsive genes in rice roots.ammonium has long been used as the predominant form of nitrogen source for paddy rice (oryza sativa). recently, increasing evidence suggests that nitrate also plays an important role for nitrogen acquisition in the rhizosphere of waterlogged paddy rice. ammonium and nitrate have a synergistic effect on promoting rice growth. however, the molecular responses induced by simultaneous treatment with ammonium and nitrate have been less studied in rice. here, we performed transcriptome analysis to ide ...201729203827
osofp19 modulates plant architecture by integrating the cell division pattern and brassinosteroid signaling.characterization of ovate family proteins (ofps) has revealed that they exert functions by interacting with different types of transcription factor. however, the molecular bases of these processes are poorly understood. here, we report that osofp19 negatively modulates brassinosteroid (br) response and integrates it with the cell division pattern to affect plant architecture, including grain shape, through interaction with both dwarf and low-tillering (dlt) and oryza sativa homeobox1 (osh1). ove ...201829205590
contrasting patterns of variation in weedy traits and unique crop features in divergent populations of us weedy rice (oryza sativa sp.) in arkansas and california.weed evolution from crops involves changes in key traits, but it is unclear how genetic and phenotypic variation contribute to weed diversification and productivity. weedy rice is a conspecific weed of rice (oryza sativa) worldwide. we used principal component analysis and hierarchical clustering to understand how morphologically and evolutionarily distinct us weedy rice populations persist in rice fields in different locations under contrasting management regimes. further, we used a representat ...201729205860
reproducible genomic dna preparation from diverse crop species for molecular genetic applications.several high-throughput molecular genetic analyses rely on high-quality genomic dna. copurification of other molecules can negatively impact the functionality of plant dna preparations employed in these procedures. isolating dna from agronomically important crops, such as sugarcane, rice, citrus, potato and tomato is a challenge due to the presence of high fiber, polysaccharides, or secondary metabolites. we present a simplified, rapid and reproducible sds-based method that provides high-quality ...201729213298
evolution of microrna827 targeting in the plant kingdom.unlike most ancient micrornas, which conservatively target homologous genes across species, microrna827 (mir827) targets two different types of spx (syg1/pho81/xpr1)-domain-containing genes, nitrogen limitation adaptation (nla) and phosphate transporter 5 (pht5), in arabidopsis thaliana and oryza sativa to regulate phosphate (pi) transport and storage, respectively. however, how mir827 shifted its target preference and its evolutionary history are unknown. based on target prediction analysis, we ...201729214636
identification of virus-derived sirnas and their targets in rbsdv-infected rice by deep sequencing.rna interference (rnai) is a conserved mechanism against viruses in plants and animals. it is thought to inactivate the viral genome by producing virus-derived small interfering rnas (vsirnas). rice black-streaked dwarf virus (rbsdv) is transmitted to plants by the small brown planthopper (laodelphax striatellus), and seriously threatens production of rice in east asia, particularly oryza sativa japonica subspecies. through deep sequencing, genome-wide comparisons of rbsdv-derived vsirnas were m ...201729215744
illuminating the role of the gα heterotrimeric g protein subunit, rga1, in regulating photoprotection and photoavoidance in rice.we studied physiological mechanisms of photoavoidance and photoprotection of a dwarf rice mutant with erect leaves, d1, in which the rga1 gene, which encodes the gα subunit of the heterotrimeric g protein, is non-functional. leaves of d1 exhibit lower leaf temperature and higher photochemical reflectance index relative to wild type (wt), indicative of increased photoavoidance and more efficient light harvesting. rna sequencing analysis of flag leaves revealed that messenger rna levels of genes e ...201829216416
loss of lofsep transcription factor function converts spikelet to leaf-like structures in rice.sepallata (sep)-like genes, which encode a subfamily of mads-box transcription factors, are essential for specifying floral organ and meristem identity in angiosperms. rice (oryza sativa l.) has five sep-like genes with partial redundancy and overlapping expression domains, yet their functions and evolutionary conservation are only partially known. here, we describe the biological role of one of the sep genes of rice, osmads5, in redundantly controlling spikelet morphogenesis. osmads5 belongs to ...201729217592
genetic dissection and validation of candidate genes for flag leaf size in rice (oryza sativa l.).two major loci with functional candidate genes were identified and validated affecting flag leaf size, which offer desirable genes to improve leaf architecture and photosynthetic capacity in rice. leaf size is a major determinant of plant architecture and yield potential in crops. however, the genetic and molecular mechanisms regulating leaf size remain largely elusive. in this study, quantitative trait loci (qtls) for flag leaf length and flag leaf width in rice were detected with high-density ...201729218376
funricegenes dataset for comprehensive understanding and application of rice functional genes.as a main staple food, rice is also a model plant for functional genomic studies of monocots. decoding of every dna element of the rice genome is essential for genetic improvement to address increasing food demands. the past 15 years have witnessed extraordinary advances in rice functional genomics. systematic characterization and proper deposition of every rice gene are vital for both functional studies and crop genetic improvement.201829220485
comprehensive analysis and transcript profiling of arabidopsis thaliana and oryza sativa catalase gene family suggests their specific roles in development and stress responses.stress induces the generation of reactive oxygen species (ros) that ultimately hampers the growth, development, and productivity of the plant. as an antioxidant enzyme, catalase converts hydrogen peroxide to water and keeps ros level down to protect cells from stress-induced apoptosis. here, a genome-wide analysis of catalase gene family has been performed in two model plants- arabidopsis thaliana and oryza sativa. both arabidopsis and rice has a small family of three and four genes, respectivel ...201729223068
enhanced resistance to rice blast and sheath blight in rice (oryza sativa l.) by expressing the oxalate decarboxylase protein bacisubin from bacillus subtilis.oxalate decarboxylase (oxdc), catalyzing the degradation of oxalic acid, is widely distributed in varieties of organisms. in this study, an oxalate decarboxylase gene from bacillus subtilis strain bs-916, bacisubin, was transformed into rice variety nipponbare to generate transgenic rice with increased oxdc activity. pathogenicity test revealed that the transgenic rice showed enhanced resistance to rice blast and sheath blight. further rna-seq analysis between nipponbare wt (wild type) and trans ...201729223342
phylogenomic detection and functional prediction of genes potentially important for plant meiosis.meiosis is a specialized type of cell division necessary for sexual reproduction in eukaryotes. a better understanding of the cytological procedures of meiosis has been achieved by comprehensive cytogenetic studies in plants, while the genetic mechanisms regulating meiotic progression remain incompletely understood. the increasing accumulation of complete genome sequences and large-scale gene expression datasets has provided a powerful resource for phylogenomic inference and unsupervised identif ...201829223357
overexpression of oscipk30 enhances plant tolerance to rice stripe virus.rice stripe virus (rsv) causes a severe disease in oryza sativa (rice) in many eastern asian countries. the ns3 protein of rsv is a viral suppressor of rna silencing, but plant host factors interacting with ns3 have not been reported yet. here, we present evidence that expression of rsv ns3 in arabidopsis thaliana causes developmental abnormalities. through yeast two-hybrid screening and a luciferase complementation imaging assay, we demonstrate that rsv ns3 interacted with oscipk30, a cbl (calc ...201729225594
activity of icacinol from icacina trichantha on seedling growth of oryza sativa and arabidopsis thaliana.broadleaf weeds are very costly for crop growers. additional herbicidal compounds need to be obtained, especially from natural sources. extracts of icacina trichantha were evaluated for responses in germinating seeds and seedlings of rice (oryza sativa) and arabidopsis (arabidopsis thaliana). an ethyl acetate fraction of i. trichantha tuber and a diterpenoid constituent, icacinol (1), were found to have impacts on germination and growth of seedlings. the seed germination inhibitory activity on r ...201729227099
profiling of accessible chromatin regions across multiple plant species and cell types reveals common gene regulatory principles and new control modules.the transcriptional regulatory structure of plant genomes remains poorly defined relative to animals. it is unclear how many cis-regulatory elements exist, where these elements lie relative to promoters, and how these features are conserved across plant species. we employed the assay for transposase-accessible chromatin (atac-seq) in four plant species (arabidopsis thaliana, medicago truncatula, solanum lycopersicum, and oryza sativa) to delineate open chromatin regions and transcription factor ...201729229750
evaluation of mercury uptake and distribution in rice (oryza sativa l.).mercury (hg) contamination in soil-rice systems from industry, mining and agriculture has received increasing attention recently in china. pot experiments were conducted to research the hg accumulation capacity of rice under exogenous hg in the soil and study the major soil factors affecting translocation of hg from soil to plant. soil treated with 2 mg kg-1 hg decreased rice grain yield and inhibited the growth of rice plants. with increased hg contamination of the rice, the enrichment rate of ...201729230483
genetic dissection of leaf senescence in rice.leaf senescence, the final stage of leaf development, is a complex and highly regulated process that involves a series of coordinated actions at the cellular, tissue, organ, and organism levels under the control of a highly regulated genetic program. in the last decade, the use of mutants with different levels of leaf senescence phenotypes has led to the cloning and functional characterizations of a few genes, which has greatly improved the understanding of genetic mechanisms underlying leaf sen ...201729232920
proteome-wide analysis of lysine 2-hydroxyisobutyrylation in developing rice (oryza sativa) seeds.lysine 2-hydroxyisobutyrylation is a recently identified protein post-translational modification that is known to affect the association between histone and dna. however, non-histone protein lysine 2-hydroxyisobutyrylation remains largely unexplored. utilizing antibody-based affinity enrichment and nano-hplc/ms/ms analyses of 2-hydroxyisobutyrylation peptides, we efficaciously identified 9,916 2-hydroxyisobutyryl lysine sites on 2,512 proteins in developing rice seeds, representing the first lys ...201729235492
the e3 ligase drought hypersensitive negatively regulates cuticular wax biosynthesis by promoting the degradation of transcription factor roc4 in rice.cuticular wax plays crucial roles in protecting plants from environmental stresses, particularly drought stress. many enzyme-encoding genes and transcription factors involved in wax biosynthesis have been identified, but the underlying posttranslational regulatory mechanisms are poorly understood. here, we demonstrate that drought hypersensitive (dhs), encoding a really interesting new gene (ring)-type protein, is a critical regulator of wax biosynthesis in rice (oryza sativa). the cuticular wax ...201729237723
cry1 bt susceptibilities of fall armyworm (lepidoptera: noctuidae) host strains.the fall armyworm, spodoptera frugiperda (smith; lepidoptera: noctuidae), is a highly polyphagous, multivoltine pest of commercial crops including corn (zea mays l.), cotton (gossypium spp. l.), rice (oryza sativa l.), and pasture grasses. fall armyworm has become a growing concern in agricultural communities across the americas as field populations in many locales have evolved resistance to several cry1 toxins derived from the bacterium bacillus thuringiensis berliner (bt). an often overlooked ...201729240921
gene mapping and transcriptome profiling of a practical photo-thermo-sensitive rice male sterile line with seedling-specific green-revertible albino leaf.abnormal environment weather can cause rice photoperiod-thermo-sensitive genic male sterile (ptgms) lines fertile or partially fertile and thus cause the mixture of true hybrids with selfing seeds. seedling-specific green-revertible albino leaf color mutant can be used to distinguish the real hybrids. besides, it can also be used as an ideal material to research the development of chloroplast and biosynthesis of chlorophyll. the phenotype of leaf color mutants includes light green, yellowing, al ...201829241565
genome-wide assessment of polygalacturonases-like (pgl) genes of medicago truncatula, sorghum bicolor, vitis vinifera and oryza sativa using comparative genomics approach.the polygalacturonases (pg) is one of the important members of pectin-degrading glycoside hydrolases of the family gh28. in plants, pg represents multigene families associated with diverse processes. in the present study, an attempt has been made to investigate the diversity of pg genes among monocots and dicots with respect to phylogeny, gene duplication and subcellular localization to get an insight into the evolutionary and functional attributes. the genome-wide assessment of medicago truncat ...201729243204
proteomic analysis reveals coordinated regulation of anthocyanin biosynthesis through signal transduction and sugar metabolism in black rice leaf.black rice (oryza sativa l.) is considered to be a healthy food due to its high content of anthocyanins in the pericarp. the synthetic pathway of anthocyanins in black rice grains has been identified, however, the proteomic profile of leaves during grain development is still unclear. here, isobaric tags relative and absolute quantification (itraq) ms/ms was carried out to identify statistically significant changes of leaf proteome in the black rice during grain development. throughout three sequ ...201729244752
identification and expression profiling of the auxin response factors in capsicum annuum l. under abiotic stress and hormone treatments.auxin response factors (arfs) play important roles in regulating plant growth and development and response to environmental stress. an exhaustive analysis of the caarf family was performed using the latest publicly available genome for pepper (capsicum annuum l.). in total, 22 non-redundant caarf gene family members in six classes were analyzed, including chromosome locations, gene structures, conserved motifs of proteins, phylogenetic relationships and subcellular localization. phylogenetic ana ...201729244768
analysis of chloroplast differences in leaves of rice isonuclear alloplasmic lines.the chloroplast being an important organelle of plant cells could possibly be associated with plant cytoplasmic male sterility (cms). to better understand the correlation between (cms) and chloroplast, we presented a comprehensive analysis based on the changes of photosynthetic parameters, chloroplasts ultrastructure, soluble sugar and starch content, the relative expression of sugar and starch metabolism genes, and chloroplast genome in rice isonuclear alloplasmic cms lines at the flowering sta ...201729247278
root cone angle is enlarged in docs1 lrr-rlk mutants in rice.the defective in outer cell layer specification 1 (docs1) gene belongs to the leucine-rich repeat receptor-like kinase (lrr-rlk) subfamily. it has been discovered few years ago in oryza sativa (rice) in a screen to isolate mutants with defects in sensitivity to aluminum. the c68 (docs1-1) mutant possessed a nonsense mutation in the c-terminal part of the docs1 kinase domain.201729247303
reconstruction of oryza sativa indica genome scale metabolic model and its responses to varying rubisco activity, light intensity, and enzymatic cost conditions.to combat decrease in rice productivity under different stresses, an understanding of rice metabolism is needed. though there are different genome scale metabolic models (gsms) of oryza sativa japonica, no gsm with gene-protein-reaction association exist for oryza sativa indica. here, we report a gsm, osi1136 of o.s. indica, which includes 3602 genes and 1136 metabolic reactions and transporters distributed across the cytosol, mitochondrion, peroxisome, and chloroplast compartments. flux balance ...201729250098
effects of heavy metal exposure on the morphological and microscopical characteristics of the paddy plant.the objective of the study was to determine the effects of heavy metal exposure on the morphology and anatomy of paddy plant (oryza sativa l.). paddy plants were treated with single and combined close of cd and pb (5, 10, 15, 20 and 25 ppm) at 35 days after germination. the results showed leaf chlorosis, decrease in number of roots and shoot height under both single and combined treatment. heavy metal content in paddy was in the order of root>stem>grain>leaf and was highest at reproductive stage ...201629251878
the imprints of the high light and uv-b stresses in oryza sativa l. 'kanchana' seedlings are differentially modulated.high light and ultraviolet-b radiation (uv-b) are generally considered to have negative impact on photosynthesis and plant growth. the present study evaluates the tolerance potential of three cultivars of oryza sativa l. (kanchana, mattatriveni and harsha) seedlings towards high light and uv-b stress on the basis of photosynthetic pigment degradation, chlorophyll a fluorescence parameters and rate of lipid peroxidation, expressed by malondialdehyde content. surprisingly, it was revealed that kan ...201829253814
characterization of a major qtl for manganese accumulation in rice grain.some diets lack sufficient manganese (mn), an essential mineral. increasing mn in grain by biofortification could prevent mn deficiency, but may increase levels of the toxic element cadmium (cd). here, we investigated mn in rice (oryza sativa) grains in recombinant inbred lines (rils) from the cross of 93-11 (low grain mn) with pa64s (high grain mn). quantitative trait locus (qtl) analysis to identify loci controlling grain mn identified a major qtl, qgmn7.1, on the short arm of chromosome 7; qg ...201729255144
corrigendum: involvement of polyamine oxidase-produced hydrogen peroxide during coleorhiza-limited germination of rice seeds.[this corrects the article on p. 1219 in vol. 7, pmid: 27570530.].201729255474
domesticated, genetically engineered, and wild plant relatives exhibit unintended phenotypic differences: a comparative meta-analysis profiling rice, canola, maize, sunflower, and pumpkin.agronomic management of plants is a powerful evolutionary force acting on their populations. the management of cultivated plants is carried out by the traditional process of human selection or plant breeding and, more recently, by the technologies used in genetic engineering (ge). even though crop modification through ge is aimed at specific traits, it is possible that other non-target traits can be affected by genetic modification due to the complex regulatory processes of plant metabolism and ...201729259610
the microtubule-associated ring finger protein 1 (osmar1) acts as a negative regulator for salt-stress response through the regulation of ocpi2 (o. sativa chymotrypsin protease inhibitor 2).our results suggest that a rice e3 ligase, osmar1, physically interacts with a cytosolic protein ocpi2 and may play an important role under salinity stress. salt is an important abiotic stressor that negatively affects plant growth phases and alters development. herein, we found that a rice gene, osmar1 (oryza sativa microtubule-associated ring finger protein 1), encoding the ring e3 ligase was highly expressed in response to high salinity, water deficit, and aba treatment. fluorescence signals ...201729260397
stress-responsive regulation of long non-coding rna polyadenylation in oryza sativa.recently, long non-coding rnas (lncrnas) have been demonstrated to be involved in many biological processes of plants; however, a systematic study on transcriptional and, in particular, post-transcriptional regulation of stress-responsive lncrnas in oryza sativa (rice) is lacking. we sequenced three types of rna libraries (poly(a)+, poly(a)- and nuclear rnas) under four abiotic stresses (cold, heat, drought and salt). based on an integrative bioinformatics approach and ~200 high-throughput data ...201729265542
change in lignin structure, but not in lignin content, in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis.we previously succeeded to enhance formation of wood in transgenic poplar plants by overexpressing secondary wall nac domain protein 1 from oryza sativa (osswn1), a transcription factor master regulator of secondary cell wall formation in rice, under control of the fiber preferential nst3/snd1 promoter from arabidopsis. transgenic plants had an increased cell wall thickness and cell wall density of individual cells in the secondary xylem of stems as well as an increased wood density. osswn1 trig ...201729266248
receptor-like kinase lyk9 in pisum sativum l. is the cerk1-like receptor that controls both plant immunity and am symbiosis development.plants are able to discriminate and respond to structurally related chitooligosaccharide (co) signals from pathogenic and symbiotic fungi. in model plants arabidopsis thaliana and oryza sativa lysm-receptor like kinases (lysm-rlk) atcerk1 and oscerk1 (chitin elicitor receptor kinase 1) were shown to be involved in response to co signals. based on phylogenetic analysis, the pea pisum sativum l. lysm-rlk pslyk9 was chosen as a possible candidate given its role on the cerk1-like receptor. the knock ...201729267197
genome-wide searches and molecular analyses highlight the unique evolutionary path of flavone synthase i (fnsi) in apiaceae.flavone synthase is a key enzyme for flavone biosynthesis and is encoded by two gene families, flavone synthase i (fnsi) and flavone synthase ii (fnsii). fnsii is widely distributed in plants, while fnsi has been reported in rice (oryza sativa) and seven species of apiaceae. fnsi has likely evolved from the duplication of flavanone 3β-hydroxylase (f3h). in this study, we used multiple bioinformatics tools to identify putative fnsi and f3h genes from 42 publicly available genome and transcriptome ...201729268034
identification of trna nucleoside modification genes critical for stress response and development in rice and arabidopsis.modification of nucleosides on transfer rna (trna) is important either for correct mrna decoding process or for trna structural stabilization. nucleoside methylations catalyzed by mtase (methyltransferase) are the most common type among all trna nucleoside modifications. although trna modified nucleosides and modification enzymes have been extensively studied in prokaryotic systems, similar research remains preliminary in higher plants, especially in crop species, such as rice (oryza sativa). ri ...201729268705
high temperature-induced expression of rice α-amylases in developing endosperm produces chalky grains.global warming impairs grain filling in rice and reduces starch accumulation in the endosperm, leading to chalky-appearing grains, which damages their market value. we found previously that high temperature-induced expression of starch-lytic α-amylases during ripening is crucial for grain chalkiness. because the rice genome carries at least eight functional α-amylase genes, identification of the α-amylase(s) that contribute most strongly to the production of chalky grains could accelerate effici ...201729270189
overcoming the species hybridization barrier by ploidy manipulation in the genus oryza.in most eudicot and monocot species, interspecific and interploidy crosses generally display abnormalities in the endosperm that are the major cause of a post-zygotic hybridization barrier. in some eudicot species, however, this type of hybridization barrier can be overcome by the manipulation of ploidy levels of one parental species, suggesting that the molecular mechanisms underlying the species hybridization barrier can be circumvented by genome dosage. we previously demonstrated that endospe ...201829271099
mutation of the ossac1 gene, which encodes an endoplasmic reticulum protein with an unknown function, causes sugar accumulation in rice leaves.sugars are the most abundant organic compounds produced by plants, and can be used to build carbon skeleton and generate energy. the sugar accumulation 1 (ossac1) gene encodes a protein with an unknown function that exhibits four n-terminal transmembrane regions and two conserved domains duf4220 and duf594 with unknown functions. ossac1 was found poorly and specifically expressed at the bottoms of young leaves and in the developing leaf sheathes. subcellular location results showed that ossac1 w ...201729272542
mercury in rice (oryza sativa l.) and rice-paddy soils under long-term fertilizer and organic amendment.high levels of mercury (hg), especially methylmercury (mehg), in rice is of concern due to its potential of entering food chain and the high toxicity to human. the level and form of hg in rice could be influenced by fertilizers and other soil amendments. studies were conducted to evaluate the effect of 24 years application of chemical fertilizers and organic amendments on total hg (thg) and mehg and their translocation in soil, plants, and rice grain. all treatments led to significantly higher c ...201829272715
influence of cadmium stress on root exudates of high cadmium accumulating rice line (oryza sativa l.).a hydroponic experiment with two different cadmium (cd) accumulating rice lines of lu527-8 (the high cd accumulating rice line) and lu527-4 (the normal rice line) was carried out to explore the links among cd stress, root exudates and cd accumulation. the results showed that (1) cd stress increased quantities of organic acids, but had no effect on composition in root exudates of the two rice lines. in cd treatments, the contents of every detected organic acid in root exudates of lu527-8 were 1.7 ...201829276952
fine mapping of quick rooting1 and 2, quantitative trait loci increasing root length in rice.the volume that the root system can occupy is associated with the efficiency of water and nutrient uptake from soil. genetic improvement of root length, which is a limiting factor for root distribution, is necessary for increasing crop production. in this report, we describe identification of two qtls for maximal root length, quick rooting 1 (qro1) on chromosome 2 and qro2 on chromosome 6 in cultivated rice (oryza sativa l.). we measured the maximal root length in 26 lines carrying chromosome se ...201729279303
nanoparticle size and production efficiency are affected by the presence of fatty acids during albumin nanoparticle fabrication.we have previously identified extensive glycation, bound fatty acids and increased quantities of protein aggregates in commercially available recombinant hsa (rhsa) expressed in oryza sativa (asian rice) (osrhsa) when compared to rhsa from other expression systems. we propose these differences may alter some attributes of nanoparticles fabricated with osrhsa, as studies have associated greater quantities of aggregates with increased nanoparticle diameters. to determine if this is the case, nanop ...201729281685
how could agronomic biofortification of rice be an alternative strategy with higher cost-effectiveness for human iron and zinc deficiency in china?iron and zinc deficiencies affect human health globally, especially in developing countries. agronomic biofortification, as a strategy for alleviating these issues, has been focused on small-scale field studies, and not widely applied while lacking of cost-effectiveness analysis (cea).201729281918
small rna profiling for identification of mirnas involved in regulation of saponins biosynthesis in chlorophytum borivilianum.micrornas act as molecular regulator of cell signaling, plant growth and development, and regulate various primary and secondary plant metabolic processes. in the present study, deep sequencing of small rnas was carried out to identify known and novel mirnas from pharmaceutically important plant, chlorophytum borivilianum.201729281971
characterization of the rice nla family reveals a key role for osnla1 in phosphate homeostasis.phosphate (pi), an essential mineral nutrient for plant development and reproduction, is one of the main components of fertilizers in modern agriculture. previous research demonstrated that atnla1 mediates ubiquitination of pi transporters in the plasma membrane and triggers their endocytosis and degradation in arabidopsis. in this study, we researched the function of nla homologous proteins in pi homeostasis in rice.201729282559
oryza sativa salt-induced ring e3 ligase 2 (ossirp2) acts as a positive regulator of transketolase in plant response to salinity and osmotic stress.a rice gene (ossirp2) encoding the ring ub e3 ligase was highly induced under salinity stress and physically interacted with a transketolase (ostkl1). overexpression of ossirp2 conferred salinity and osmotic stress tolerance in plants. the ring e3 ligases play a vital role in post transitional modification through ubiquitination-mediated protein degradation that mediate plants responses during abiotic stresses and signal transduction. in this study, we report an oryza sativa salt induced really ...201729285618
rice reduces mn uptake in response to mn stress.rice (oryza sativa l) is one of the most mn-tolerant crops that can grow in submerged paddy fields, where the mn concentration in soil solution is very high due to reduction. although a large part of mn is transferred from the roots to the shoot in rice, the roots are constantly exposed to high mn concentrations in submerged paddies. thus, mechanisms for preventing mn overaccumulation in the cytoplasm of root cells are necessary. recently, we showed that two cation diffusion facilitators, mtp8.1 ...201829286886
dark conditions enhance aluminum tolerance in several rice cultivars via multiple modulations of membrane sterols.aluminum-sensitive rice (oryza sativa l.) cultivars showed increased al tolerance under dark conditions, because less al accumulated in the root tips (1 cm) under dark than under light conditions. under dark conditions, the root tip concentration of total sterols, which generally reduce plasma membrane permeabilization, was higher in the most al-sensitive japonica cultivar, koshihikari (ko), than in the most al-tolerant cultivar, rikuu-132 (r132), but the phospholipid content did not differ betw ...201829294038
large root angle1, encoding ospin2, is involved in root system architecture in rice.root system architecture is very important for plant growth and crop yield. it is essential for nutrient and water uptake, anchoring, and mechanical support. root growth angle (rga) is a vital constituent of root system architecture and is used as a parameter for variety evaluation in plant breeding. however, little is known about the underlying molecular mechanisms that determine root growth angle in rice (oryza sativa). in this study, a rice mutant large root angle1 (lra1) was isolated and sho ...201829294052
acyl-coa-binding protein family members in laticifers are possibly involved in lipid and latex metabolism of hevea brasiliensis (the para rubber tree).acyl-coa-binding proteins (acbps) are mainly involved in acyl-coa ester binding and trafficking in eukaryotic cells, and their various functions have been characterized in model plants, such as arabidopsis thaliana (a. thaliana), oryza sativa (rice), and other plant species. in the present study, genome-wide mining and expression analysis of acbp genes was performed on hevea brasiliensis (the para rubber tree), the most important latex-producing crop in the world.201829295704
genetic dissection of qtls and differentiation analysis of alleles for heading date genes in rice.heading date is an important agronomic trait in rice (oryza sativa l.); it determines the geographical and seasonal adaptability of the crop. single segment substitution lines (sssls) have become the preferred experimental materials in mapping functional genetic variations as the particular chromosome segments from donor genotypes can be evaluated for their impact on the phenotype in a recurrent recipient background. the phenotypic differences can be attributed to the control of quantitative tra ...201829298333
evolution of the spx gene family in plants and its role in the response mechanism to phosphorus stress.molecular and genomic studies have shown the presence of a large number of spx gene family members in plants, some of which have been proved to act in p signalling and homeostasis. in this study, the molecular and evolutionary characteristics of the spx gene family in plants were comprehensively analysed, and the mechanisms underlying the function of spx genes in p signalling and homeostasis in the model plant species arabidopsis (arabidopsis thaliana) and rice (oryza sativa), and in important c ...201829298909
characterisation of a novel quantitative trait locus, gn4-1, for grain number and yield in rice (oryza sativa l.).a novel qtl for grain number, gn4-1, was identified and fine-mapped to an ~ 190-kb region on the long arm of rice chromosome 4. rice grain yield is primarily determined by three components: number of panicles per plant, grain number per panicle and grain weight. among these traits, grain number per panicle is the major contributor to grain yield formation and is a crucial trait for yield improvement. in this study, we identified a major quantitative trait locus (qtl) responsible for rice grain n ...201829299612
disruption of ossec3a increases the content of salicylic acid and induces plant defense responses in rice.the exocyst, an evolutionarily conserved octameric protein complex involved in exocytosis, has been reported to be involved in diverse aspects of morphogenesis in arabidopsis. however, the molecular functions of such exocytotic molecules in rice are poorly understood. here, we examined the molecular function of ossec3a, an important subunit of the exocyst complex in rice. the ossec3a gene is expressed in various organs, and ossec3a has the potential ability to participate in the exocyst complex ...201729300985
identification and comparative analysis of premature senescence leaf mutants in rice (oryza sativa l.).premature leaf senescence negatively impacts the grain yield in the important monocot rice (oryza sativa l.); to understand the molecular mechanism we carried out a screen for mutants with premature senescence leaves in a mutant bank generated by ethyl methane sulfonate (ems) mutagenesis of elite indica rice zhongjian100. five premature senescence leaf (psl15, psl50, psl89, psl117 and psl270) mutants were identified with distinct yellowish phenotypes on leaves starting from the tillering stage t ...201829301377
exogenous short-term silicon application regulates macro-nutrients, endogenous phytohormones, and protein expression in oryza sativa l.silicon (si) has been known to regulate plant growth; however, the underlying mechanisms of short-term exogenous si application on the regulation of calcium (ca) and nitrogen (n), endogenous phytohormones, and expression of essential proteins have been little understood.201829301510
multiple origin but single domestication led to oryza sativa.the domestication scenario that led to asian rice (oryza sativa) is a contentious topic. here, we have reanalyzed a previously published large-scale wild and domesticated rice dataset, which were also analyzed by two studies but resulted in two contrasting domestication models. we suggest the analysis of false positive selective sweep regions and phylogenetic analysis of concatenated genomic regions may have been the sources that contributed to the different results. in the end, our result indic ...201829301862
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