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enzymic properties of ribulose-1,5-bisphosphate carboxylase/oxygenase purified from rice leaves.the enzymic properties of ribulose 1,5-bisphosphate (rubp) carboxylase/oxygenase purified from rice (oryza sativa l.) leaves were studied. rice rubpcarboxylase, activated by preincubation with co(2) and mg(2+) like other higher plant carboxylases, had an activation equilibrium constant (k(c)k(mg)) of 1.90 x 10(5) to 2.41 x 10(5) micromolar(2) (ph 8.2 and 25 degrees c). kinetic parameters of carboxylation and oxygenation catalyzed by the completely activated enzyme were examined at 25 degrees c a ...198516664401
genetic variations in the hybrids of rice (oryza sativa) and sorghum (sorghum vulgare).some of results from morphological and cytological observations and esterase-isozyme studies of a rice-sorghum hybrid are presented in this paper. there is a great diversity of morphological characters and some special characteristics in the progenies of the hybrids of rice with sorghum. the meiosis of pollen mother cells in the early generations of the hybrid was found to be abnormal. one main band coinciding with one found in sorghum but lacking in rice appeared in the majority of the hybrid l ...198524253065
diallel analysis of submergence tolerance in rice, oryza sativa l.the genetics of submergence tolerance in rice was studied in a 10 × 10 half-diallel cross set involving 10 lowland rice varieties, four of which were tolerant ('fr13a', 'fr43b', 'kurkaruppan', and 'goda heenati') and the remaining six were nontolerant ('rd19', 'ir42', 'ir17494-32-1', 'ir19672-24-3', 'jagannath', and 'cr1009'). estimates of genetic parameters following hayman's method showed significant additive and nonadditive gene action and the latter appeared to be solely due to dominance. na ...198524253055
interaction of rice (oryza sativa) lectin with n-acetylglucosaminides. fluorescence studies.the interaction of lectin isolated from rice (oryza sativa) embryos with n-acetylglucosaminides was studied by equilibrium dialysis and fluorescence. equilibrium dialysis with 4-methylumbelliferyl-(glcnac)2 showed that rice lectin (mr 38000) contains four equivalent saccharide-binding sites. addition of the n-acetylglucosaminides glcnac, (glcnac)2 and (glcnac)3 enhanced the intrinsic fluorescence of rice lectin and this was accompanied by a 10nm blue-shift of its maximum fluorescence with (glcna ...19854052018
photosynthetic development of anaerobically grown rice (oryza sativa) after exposure to air.during anaerobic germination, rice produces a coleoptile devoid of carotenoid and chlorophyll. further development and greening of the shoot occur upon exposure of the seedlings to air. in this study, a comparison was made between anaerobically (n(2)) germinated rice, greened upon exposure to air, and air/dark (a/d) germinated seedlings, greened upon exposure to light. after exposure to air, n(2)-grown seedlings had a 76-hour lag before net oxygen evolution occurred compared to a 6-hour lag for ...198516664275
interrelationship of cultivated rices oryza sativa and o. glaberrima with wild o. perennis complex : analysis of fraction 1 protein and some repeated dna sequences.phylogenetic relationship of the cultivated rices oryza sativa and o. glaberrima with the o. perennis complex, distributed on the three continents of asia, africa and america, and o. australiensis has been studied using fraction 1 protein and two repeated dna sequences as markers. fraction 1 protein isolated from the leaf tissue of accessions of different species was subjected to isoelectric focusing. all the species studied have similar nuclear-encoded small subunit polypeptides and chloroplast ...198524254178
inheritance of callus formation ability in anther cultures of rice, oryza sativa l.inheritance of ability to form callus in rice anther culture was studied using the diallel technique. anthers containing uninucleate microspores from two japonica cultivais ('minehikari' and 'taipei 309'), two indica cultivars ('mingolo' and 'suweon 290'), and 12 f1's of the diallel crosses involving these four parents were cultured on chaleffs r2 medium and evaluated for callus induction. the parents showed significant differences in anther callus formation, from 41.9% ('taipei 309') to 0% ('su ...198524254166
effect of zinc deficiency on kinetics of ribulose-1,5-biphosphate carboxylase of oryza sativa l. and pennisetum americanum (l.) leeke. 19854030003
effect of submergence on translocation, starch content and amylolytic activity in deep-water rice.submergence induces rapid internodal elongation in deep-water rice (oryza sativa l. cv. habiganj aman ii). we investigated the metabolic activities which help to support such fast growth. three days of submergence in water under continuous light led to the mobilization of 65% of the starch from those regions of rice internodes which had been formed prior to submergence. disappearance of starch was accompanied by a 70-fold enhancement of amylolytic activity. similar increases in amylolytic activi ...198424253273
role of gibberellin in the growth response of submerged deep water rice.we have shown previously that ethylene, which accumulates in the air spaces of submerged stem sections of rice (oryza sativa l. cv "habiganj aman ii"), is involved in regulating the growth response caused by submergence. the role of gibberellins in the submergence response was studied using tetcyclacis (tcy), a new plant growth retardant, which inhibits gibberellin biosynthesis. stem sections excised from plants that had been watered with a solution of 1 micromolar tcy for 7 to 10 days did not e ...198416663977
genetic control of meiosis in rice, oryza sativa l. iii. effect of ds genes on genetic recombination.the recombination frequency as influenced by five independent recessive ds genes was measured on three segments of different chromosomes of rice, oryza sativa l. each ds gene in the homozygous condition resulted in an almost equally reduced recombination frequency in the three segments. when the mean reduction in recombination frequency was related to the reduction of chiasma frequency, the five ds genes were divided into two types: in one type the reduction of chiasma frequency almost correspon ...198417246240
cytochrome oxidase subunit ii gene of rice has an insertion sequence within the intron.we have isolated and sequenced the cytochrome oxidase subunit ii gene from rice (oryza sativa l. var labelle). the overall structural organization of this gene is very similar to that of the maize gene. this gene contains an intron in a position identical to the intron in the maize gene. however, the intron in the rice gene is longer than that of the maize gene largely due to a 461 bp insertion sequence, which has inverted repeats at its termini and is flanked by direct repeats, characteristic o ...19846093039
hydrolysis of conjugated gibberellins by β-glucosidases from dwarf rice (oryza sativa l. cv. «tan-ginbozu»).dwarf rice (oryza sativa l. cv. «tan-ginbozu») is widely used for gibberellin conjugate bioassay. soluble and particulate fractions from seeds and seedlings of this variety showed hydrolytic activity toward [(3)h]ga-o-glc. the soluble fraction from mature seeds exhibited the highest [(3)h]ga-o-glc/pnp-β-glc hydrolysis ratio which decreased during the early germination phase. the soluble β-glucosidases from mature seeds were efficiently separated by cmsephadex c-50 column chromatography. it was f ...198423195047
effect of submergence on the cell wall composition of deep-water rice internodes.the cell wall composition of internodes of deep-water rice plants (oryza sativa l. cv habiganj aman ii) which were induced to grow rapidly by submergence in water was compared to that of nonsubmerged plants which grew slowly. no differences could be detected in cellulose, uronic acid, and lignin content expressed on a dry weight basis. cell wall preparations of rapidly growing, submerged internodes contained more hydroxyproline and had a higher hydration capacity than those of control internodes ...198416663779
subcellular site of lectin synthesis in developing rice embryos.embryos of developing rice (oryza sativa l. cv. koshihikari) caryopses which actively synthesize lectin were labelled with [s]cysteine for different times and newly synthesized rice lectin was isolated by affinity chromatography. gel filtration of embryo extracts on sepharose-4b indicated that a large portion of the labelled lectin was associated with the particulate fraction. experiments with detergent indicated that this lectin was sequestered within organelles. when extracts of pulse-labelled ...198416453545
cell-cycle dependency of radiosensitivity and mutagenesis in fertilized egg cells of rice, oryza sativa l. : 2. x-ray sensitivity and mutation rate during a cell cycle.in order to examine changes in survival and mutation rates during a cell cycle in higher plant, fertilized egg cells of rice were irradiated with x-rays at 2 h intervals for the first 36 h after pollination, i.e., at different phases of the first and second cell cycles. the most sensitive phase in lethality was late g1 to early s, followed by late g2 to m, which were more sensitive than the other phases. in both m1 and m2 generations, sterile plants appeared most frequently when fertilized egg c ...198424257637
influence of osmotic adjustment on leaf rolling and tissue death in rice (oryza sativa l.).osmotic adjustment, measured by the lowering of the osmotic potential at full turgor, and its influence on leaf rolling and leaf death was assessed in the lowland rice (oryza sativa l.) cultivar ir36 in both the greenhouse and field. the degree of osmotic adjustment varied with the degree and duration of stress, but was usually 0.5 to 0.6 megapascal (maximally 0.8 to 0.9 megapascal) under severe stress conditions. in leaves in which osmotic adjustment was 0.5 to 0.6 megapascal, leaf rolling and ...198416663622
nonosmotic effects of polyethylene glycols upon sodium transport and sodium-potassium selectivity by rice roots.addition of polyethylene glycol (peg) as an osmotic agent (at -230 kilopascals) dramatically lessened the toxicity of nacl (at 50 moles per cubic meter) to rice (oryza sativa l.) seedlings. this was explained by a reduction in the uptake of nacl. this reduction was much greater than could be accounted for by the lowered transpiration rate resulting from the solute potential changes due to the peg.low concentrations of peg (-33 kilopascals and less) had no effect upon transpiration rate but reduc ...198416663615
primary trisomics of rice: origin, morphology, cytology and use in linkage mapping.twelve primary trisomics of oryza sativa l. were isolated from the progenies of spontaneous triploids and were transferred by backcrossing to the genetic background of ir36, a widely grown high yielding rice variety. eleven trisomics can be identified morphologically from one another and from diploids. however, triplo 11 is difficult to distinguish from diploid sibs.-the extra chromosome of each trisomic was identified cytologically at pachytene stage of meiosis, and the chromosomes were numbere ...198417246212
possible roles of calcium and calmodulin in the biosynthesis and secretion of alpha-amylase in rice seed scutellar epithelium.the scutellar epithelial cells of rice (oryza sativa l. cv kimmazé) seeds actively secrete alpha-amylase in an early stage of germination. employing an in vivo system of freshly dissected scutellar tissues, effect of ca(2+) on the biosynthesis and the secretion of alpha-amylase have been studied. the maximum biosynthetic rate was saturated at about 0.5 mm external ca(2+) concentrations, whereas the secretion continued to increase to concentrations above 10 mm ca(2+). in the presence of 1 mm ca(2 ...198416663573
abscisic acid promotes lectin biosynthesis in developing and germinating rice embryos.immature rice (oryza sativa, l) embryos isolated about 12 days post anthesis are fully able to develop into young seedlings when cultured in vitro. concomitantly, they rapidly loose their lectin synthesis activity. abscisic acid added to the nutrient medium prevents precocious germination of the immature embryos and simultaneously strongly promotes lectin biosynthesis activity. similarly, abscisic acid keeps mature embryos grown in a nutrient medium in a dormant state and maintains their lectin ...198424253393
the cellular basis of the elongation response in submerged deep-water rice.the cellular basis of internode elongation was studied in intact deep-water rice plants (oryza sativa l. cv. "habiganj aman ii") and in isolated stem sections. in intact plants, growth was stimulated by submergence in water and by ethylene treatment. in isolated sections, growth was enhanced by submergence, by ethylene, and by exposure of the tissue to an atmosphere of 3% o2, 91% n2 and 6% co2 or 3% o2, 91% n2, 6% co2 and 1 μl l(-1) c2h4 (by vol.). under all these conditions, growth was localize ...198424258374
regulation of growth in stem sections of deep-water rice.submergence in water greatly stimulates internodal elongation in excised stem sections of deep-water rice (oryza sativa l. cv. "habiganj aman ii") and inhibits growth of leaf blades and leaf sheaths. the highest rates of internodal growth have been observed in continuous light. very little growth occurs in submerged sections kept in darkness or incubated under n2 in the light. the effect of submergence on the growth of deep-water rice is, at least in part, mediated by c2h4, which accumulates in ...198424258373
identification and localization of sugar components of rice (oryza sativa l.) root cap mucilage.this study is devoted to the neutral sugar analysis of rice root exudate and root cap mucilage.the results indicate that most of the carbohydrate in the exudate is released in soluble dialysable form, mainly as glucose. mucilage polymers represent only a minor fraction of the whole exudate containing glucose, galactose, xylose, arabinose, and minor amounts of fucose and mannose. in situ localization of some of these sugar residues is also reported. the strongest reaction detected is with fucose- ...198423195302
lectin accumulation and synthesis in developing rice embryos.rice (oryza sativa, l.) lectin is specifically synthesized during seed formation. it accumulates very rapidly between 8 and 16 days post anthesis and reaches its final content 20 days post anthesis. the rate of synthesis of this protein dramatically increases during the developmental phase coinciding with the rapid lectin accumulation. when the lectin accumulation stops, its rate of synthesis declines rapidly and eventually stops in fully mature embryos. it appears, therefore, that rice lectin i ...198324258184
photosynthesis and ribulose 1,5-bisphosphate carboxylase in rice leaves: changes in photosynthesis and enzymes involved in carbon assimilation from leaf development through senescence.changes in photosynthesis and the ribulose 1,5-bisphosphate (rubp) carboxylase level were examined in the 12th leaf blades of rice (oryza sativa l.) grown under different n levels. photosynthesis was determined using an open infrared gas analysis system. the level of rubp carboxylase was measured by rocket immunoelectrophoresis. these changes were followed with respect to changes in the activities of rubp carboxylase, ribulose 5-phosphate kinase, nadp-glyceraldehyde 3-phosphate dehydrogenase, an ...198316663318
somaclonal genetics of rice, oryza sativa l.the inheritance and variations of some traits of more than 2,000 somatic cell derived plants of rice (oryza sativa l.) were investigated in the second and third generations (t2 and t3) of regenerated plants (somaclones). the percentages of multiploids occurring in somaclones ranged from 0-13.3 in nine varieties (or hybrids) of 'hsien' (indica) group, but no multiploid was found in nine varieties (or hybrid) of 'keng' (japonica) group. a dwarf mutant with the height of only 20 cm was isolated in ...198324258482
seed dormancy in red rice : iii. response to nitrite, nitrate, and ammonium ions.sodium nitrite at 10 millimolar breaks dormancy of dehulled red rice (oryza sativa). while germination is light independent, low ph conditions (ph 3) are required for maximum response. water and buffer controls at ph 3 remain dormant. the response to nitrite occurs at 25 and 30 degrees c but is reduced at 20 degrees c, although nondormant seeds germinate readily at this temperature. the contact time for response to nitrite is less than 2 h at the start of imbibition. seeds imbibed first in water ...198316663224
tryptophan enhancement of somatic embryogenesis in rice.cereal embryos can produce two types of callus. one type, termed "embryogenic," consists of small meristematic-like cells and gives rise to many plants by somatic embryogenesis if placed on a suitable regeneration medium. the other is termed "nonembryogenic" and consists of long tubular cells which gives rise to few or no plants. high concentrations of tryptophan increased the formation of embryogenic callus in three rice cultivars (oryza sativa l. calrose 76, pokkali, and ir 36) but not in four ...198316663163
inactivation of alcohol dehydrogenase in rice seedlings is related to a microsomal fatty acid alpha-oxidation system.the selective inactivation of alcohol dehydrogenase by the inactivator found in the microsomal fraction of rice (oryza sativa) seedlings growing in air (shimomura, s. & beevers, h. (1983) plant physiol. 71, 736-741; 742-746) was further studied. this inactivation was found to be essentially dependent on the presence of free fatty acids. the specificity for fatty acids and the inhibitory effects of imidazole, 2-hydroxyfatty acids and dithiothreitol on the inactivation were all consistent with the ...19862939066
chilling sensitivity in oryza sativa: the role of protein phosphorylation in protection against photoinhibition.the effects of exposure to low temperature on photosynthesis and protein phosphorylation in chilling-sensitive and cold-tolerant plant species were compared. chilling temperatures resulted in light-dependent loss of photosynthetic electron transport in chilling-sensitive rice (oryza sativa l.) but not in cold-tolerant barley (hordeum vulgare l.). brief exposure to chilling temperatures (0-15 degrees c, 10 min) did not cause a significant difference in photosynthetic o(2) evolution capacity in vi ...198616664636
a comparison of the submergence response of deepwater and non-deepwater rice.twelve cultivars of rice (oryza sativa l.), representing deepwater, short-statured, and semidwarf types, were tested for their response to submergence. the magnitude of the response varied between cultivars; however, all cultivars responded to submergence by rapid growth once internodal elongation had started. three of these cultivars were tested for elongation capacity at four ages. the deepwater rice was capable of rapid internodal elongation in response to submergence at 4 weeks of age. growt ...198616664647
cell-cycle dependency of radiosensitivity and mutagenesis in fertilized egg cells of rice, oryza sativa l. : 1. autoradiographic determination of the first dna synthetic phase.to determine the time and duration of the first and second dna synthetic phases in fertilized egg cells and central cells of rice, a total of 753 ovules were sampled at 2 h intervals during the first 30 h after pollination and exposed to (3)h-thymidine for 2 h at 25 °c. autoradiographic observation of labeled nuclei was made for fertilized egg cells, as well as for central and antipodal cells. the first and second dna synthetic phases in fertilized egg cells were found 8-12 h and 21-25 h after p ...198324263540
the role of ethylene in the growth response of submerged deep water rice.we investigated the effect of partial submergence on internode elongation in a bangladesh variety of floating or deep water rice (oryza sativa l., cv. habiganj aman ii). in plants which were at least 21 days old, 7 days of submergence led to a 3- to 5-fold increase in internodal length. during submergence, the ethylene concentration in the internodes increased from about 0.02 to 1 microliters per liter. treatment of nonsubmerged plants with ethylene also stimulated internode elongation. when eth ...198316663022
subcellular localization of rice leaf aryl acylamidase activity.the intracellular localization of aryl acylamidase (aryl-acylamide amidohydrolase, ec 3.5.1.13) in rice (oryza sativa l. var starbonnet) leaves was investigated. the enzyme hydrolyzes and detoxifies the herbicide propanil (3,4-dichloropropionanilide) thereby accounting for immunity of the rice plant to herbicidal action. fractionation of mesophyll protoplasts by differential centrifugation yielded the highest specific activity of amidase in the crude mitochondrial fraction. further separation of ...198316662987
adaptation of ribonucleic acid metabolism to anoxia in rice embryos.rice (oryza sativa var. cigalon) is a plant which can adapt to very stringent anoxic conditions. it has previously been shown that during the adaptation period the energy charge can be used as a marker for metabolic activity. we have studied rna metabolism during this period and correlated it with changes in the energy charge. uptake of labeled precursor, utp-specific activity, and incorporation were measured. immediately after transfer to anaerobic conditions, the utp pool size is reduced and t ...198316662943
alcohol dehydrogenase and an inactivator from rice seedlings.alcohol dehydrogenase (adh) was measured in the various organs of rice seedlings (oryza sativa) growing in air. in extracts from ungerminated seeds, the adh is stable, but in extracts from seedlings more than 2 days old the enzyme initially present loses activity in a time- and temperature-dependent fashion, due to the presence of an inactivating component which increases with age in roots and shoots. the inactivation can be prevented completely by dithiothreitol, and when this is included in th ...198316662898
enzymic mechanism of starch breakdown in germinating rice seeds : 15. immunochemical study on multiple forms of amylase.the formation of multiple forms of amylases in germinating rice (oryza sativa l. cv kimmaze) grains was examined by means of isoelectric focusing, cross-immunoelectrophoresis, and rocket-line immunoelectrophoresis followed by a reaction of enzymic characterization by using beta-limit dextrin or starch as substrate. the constituents detected by isoelectric focusing were identified as three electrophoretically heterogeneous antigens. the major alpha-amylase bands a and b corresponded to a same ant ...198316662805
heterosis breeding in rice (oryza sativa l.).studies conducted at the international rice research institute (irri) during 1980 and 1981 have shown up to 73% heterosis, 59% heterobeltiosis and 34% standard heterosis for yield in rice. the latter was estimated in comparison to commercial varieties: ir36 and ir42 (yield 4-5 t/ha in wet season trials and 7-8 t/ha in dry season trials). generally speaking, absolute yield was lower and extent of standard heterosis was higher in wet season than in dry season with some exception. yields up to 5.9 ...198224270875
embryogenesis in callus derived from rice microspores.differentiating calli derived from rice (oryza sativa l.) microspores were examined histologically. shoot and root meristems were observed to be arising by both organogenesis as well as embryogenesis. embryoid attachment to callus (as well as other embryoids) was at the scutellum adjacent to the mesocotyl and radicle. these observations could be interpreted as an indication of the totipotent plasticity of that tissue.198224257769
occurrence of nitrate reductase inhibitor in rice plants.nitrate reductase inhibitor is usually found in the roots of rice plants (oryza sativa l. cv mr7), but it was also produced in the shoots of aging plants. the inhibitor was inducible in the shoot of rice seedlings by dark, minus-nitrate or plus-ammonium treatments. there appears to be a general involvement of the inhibitor in the control of nitrate assimilation in the plant.198216662758
biosynthesis of storage proteins in developing rice seeds.sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of the starchy endosperm protein of rice (oryza sativa l. japonica cv koshihikari) during seed development confirmed that storage protein begins to accumulate about 5 days after flowering. two polypeptide groups, 22 to 23 and 37 to 39 kilodaltons, the components of glutelin, the major storage protein in rice seed, appeared 5 days after flowering. a 26-kilodalton polypeptide, the globulin component, also appeared 5 days after flow ...198216662620
light inhibition of the conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene in leaves is mediated through carbon dioxide.the mechanism of light-inhibited ethylene production in excised rice (oryza sativa l.) and tobacco (nicotiana tabacum l.) leaves was examined. in segments of rice leaves light substantially inhibited the endogenous ethylene production, but when co2 was added into the incubation flask, the rate of endogenous ethylene production in the light increased markedly, to a level which was even higher than that produced in the dark. carbon dioxide, however, had no appreciable effect of leaf segments incub ...198224271776
mechanical properties of the rice panicle.curvature, bending moment, and second moment of stem cross-sectional area were evaluated from photographic data and used to compute flexural rigidity and young's modulus in the panicle rachis of rice, oryza sativa l. ;m-101.' flexural rigidity c, and its components e, young's modulus, and i, the moment of inertia of the area about the neutral axis, were evaluated 1.5 cm (tip), 9.5 cm (mid), and 16.5 cm (base) from the tip of the panicle rachis. in dynes per square centimeter, c increases from 1. ...198216662516
enzymic mechanism of starch breakdown in germinating rice seeds : 12. biosynthesis of alpha-amylase in relation to protein glycosylation.the biosynthetic mechanism of alpha-amylase synthesis in germinating rice (oryza sativa l. cv. kimmazé) seeds has been studied both in vitro and in vivo. special attention has been focused on the glycosylation of the enzyme molecule. tunicamycin was found to inhibit glycosylation of alpha-amylase by 98% without significant inhibition of enzyme secretion. the inhibitory effect exerted by the antibiotic on glycosylation did not significantly alter enzyme activity.in an in vitro system using poly-( ...198216662436
response of excised embryos of rice (oryza sativa l.) to x-rays.the response of rice (oryza sativa l.) embryos to x-rays (m1 to m3) was studied. by means of irradiating excised embryos, both chlorophyll and macromutation were successfully induced in three genotypes of rice. however, differential responses in terms of mutation frequency, mutation spectrum and optimal levels of x-rays required for induction of mutation (chlorophyll as well as morphological) were found to exist between cultivars. in 'satika' and 'ashkhata', ld50 values and maximum induced seed ...198224270335
senescence of rice leaves : vii. proline accumulation in senescing excised leaves.proline content increased greatly in detached rice (oryza sativa cv. taichung native 1) leaves during senescence. there was a slight but significant increase in proline level after one day of incubation, and, subsequently, proline accumulated relatively rapidly. by 4 days after excision, the level of proline had increased 30- to 50-fold, which is similar to the level seen in the water-stressed detached rice leaves. it is unlikely that the proline accumulation in detached leaves is to be derived ...198216662400
physiology of root-associated nitrogenase activity in oryza sativa.an intact method for measuring immediately linear rates of acetylene reduction was used to investigate the relationship between temperature, ph, o(2) concentration, and light intensity with the rate of root-associated nitrogenase activity in rice (oryza sativa l.). nitrogenase activity varied over a temperature range of 10 to 50 degrees c and optimal rates of acetylene reduction were recorded at 35 degrees c. nitrogenase activity was also influenced by the ph of the liquid surrounding the roots ...198216662363
glutamate synthase from rice leaves.ferredoxin-dependent glutamate synthase (ec 1.4.7.1) from rice leaves (oryza sativa l. cv delta) was purified 206-fold with a final specific activity of 35.9 mumoles glutamate formed per min per milligram protein by a procedure including ammonium sulfate fractionation, deae-cellulose chromatography, sephacryl s-300 gel filtration, and ferredoxin-sepharose affinity chromatography. the purified enzyme yielded a single protein band on polyacrylamide gel electrophoresis. molecular weight of the nati ...198216662307
rice protein-body formation: all types are initiated by dilation of the endoplasmic reticulum.the ultrastructure of protein deposition in the starchy endosperm of developing rice (oryza sativa l.) grains was examined in conventionally fixed (glutaraldehyde and osmium tetroxide) tissues and also in thick sections (0.3 μm) of zinc iodide-osmium tetroxide post-fixed tissue. three types of previously characterised protein body were observed and it was shown that each type was initiated by dilations of the endoplasmic reticulum. crystalline type protein bodies were initiated by a ribosome-fre ...198224275981
studies on protein characteristics in rice mutants.twenty mutants isolated from "latisail", "jhingasail" and "pankaj" varieties of rice (oryza sativa l.) were screened for two aspects of nutritive quality, namely crude protein content and distribution pattern of protein in the endosperm. observations revealed a wide variation for both characters, and while there was no consistent association between protein content and test grain weight, which varied between varieties, a positive correlation between protein content and grain sterility was noted. ...198224271369
genetic analysis of resistance to whitebacked planthopper in twenty-one varieties of rice, oryza sativa l.the inheritance of resistance to whitebacked planthopper sogatella furcifera (horvath) was studied in 21 rice varieties. reactions of f1; f2 and f3 progenies of the crosses of 21 resistant varieties with the susceptible variety 'tn 1' revealed that a single dominant gene governs resistance in 'mushkan 41', 'santhi', 'siahnakidar 195', 'sm2-34', 'tirisurkh 251', 'zirijowaian 245', '18', '24a', '39', '76 s', '78', '180', '213 b', '267', '293', 'ci 6037-4', 'np97', 's39 jkw' and 'bansphul'. in vari ...198224271368
growth and cell wall changes in rice coleoptiles : fractionation of cell wall and changes in noncellulosic polysaccharides during intact growth.a fractionation of non-cellulosic sugars of oryza sativa l. coleoptile cell walls was carried out and the composition of each fraction was studied during coleoptile growth.percentages of fractions extracted with boiling water and with oxalate (pectic substances) were almost constant throughout development. an increase in the k ii hemicellulosic fraction (extracted with 24% koh) content, and a decrease in the k i hemicellulosic fraction (extracted with 10% koh) were detected, when coleoptile grow ...198124258865
mobilization of metabolites from leaves to grains as the cause of monocarpic senescence in rice.the pattern of senescence was studied by following the changes in chlorophyll and protein in the leaves and by measuring (32)p retention and export from source to sink during development of the rice plant (oryza sativa l. cv. jaya) subjected to different manipulative treatments. with the advance of reproductive development, the chronological sequence of leaf senescence was changed, so that the flag and the third leaf senesced earlier than did the second leaf. in presence of the daughter shoot of ...198116662105
rapid growth and apparent total nitrogen increases in rice and corn plants following applications of triacontanol.triacontanol (tria) increased fresh and dry weight and total reducible nitrogen (total n) of rice (oryza sativa l.) seedlings within 40 minutes. increases in total n in the supernatants from homogenates of corn (zea mays l.) and rice leaves treated with tria for one minute before grinding occurred within 30 and 80 minutes, respectively. the source for the increase was investigated utilizing atmospheric substitution and enrichment and depletion studies with (15)n. the increase in total n in seedl ...198116662092
ontogenetic variation of nitrogenase, nitrate reductase, and glutamine synthetase activities in oryza sativa.the relationship between the rates of nitrogenase, nitrate reductase, and glutamine synthetase activities, and plant ontogeny in rice (oryza sativa l.), cultivar ;m9', grown in salt marsh sediment with and without nitrate treatment was studied. in both treatments, nitrogenase activity measured as the immediate linear rate of acetylene reduction by bacteria associated with the roots varied with plant age. in control plants, the nitrogenase activity developed during the vegetative stage, peaked du ...198116661987
transport of assimilates in the developing caryopsis of rice (oryza sativa l.) : the pathways of water and assimilated carbon.the movement of water in the dorsal region of the developing rice caryopsis was studied using solutions of the heavy metals lanthanum and uranium. in the electron microscope electron-opaque deposits were confined to the cell walls of the pigment strand indicating that this is the main route for the water which enters and leaves the caryopsis during grain filling. the pathway of assimilates into the developing caryopsis was examined using isolated caryopses which had taken up solutions of fluores ...198124301111
heterotrophic n(2) fixation and distribution of newly fixed nitrogen in a rice-flooded soil system.rice (oryza sativa l.) plants growing in pots of flooded soil were exposed to a (15)n(2)-enriched atmosphere for 3 to 13 days in a gas-tight chamber. the floodwater and soil surface were shaded with a black cloth to reduce the activity of phototrophic n(2)-fixing micro-organisms. the highest (15)n enrichments were consistently observed in the roots, although the total quantity of (15)n incorporated into the soil was much greater. the rate of (15)n incorporation into roots was much higher at the ...198116661887
transport of assimilates in the developing caryopsis of rice (oryza sativa l.) : ultrastructure of the pericarp vascular bundle and its connections with the aleurone layer.assimilates entering the developing rice caryopsis traverse a short-distance pathway between the terminal sieve elements of the pericarp vascular bundle and the aleurone layer. the ultrastructure of this pathway has been studied. sieve elements in the pericarp vascular bundle are smaller than their companion cells.the sieve elements show few connections with surrounding vascular parenchyma elements but are connected to companion cells by compound plasmodesmata. companion cells, in turn, are conn ...198124302210
intracellular distribution of enzymes associated with nitrogen assimilation in roots.the cellular distribution of enzymes involved in nitrogen assimilation: nitrate reductase (ec 1.6.6.2), nitrite reductase (ec 1.6.6.4), glutamine synthetase (ec 6.3.1.2), glutamate synthase (ec 2.6.1.53), and glutamate dehydrogenase (ec 1.4.1.3) has been studied in the roots of five plants: maize (zea mays l. hybrid w 64a x w 182e), rice (oryza sativa l. cv. delta), bean (phaseolus vulgaris l. cv. contender), pea (pisum sativum l. cv. demi-nain), and barley (hordeum vulgare l.). initially, cell ...198124302111
[microbiological determination of choline in rice (oryza sativa)]. 19816982631
purification and characterization of acid phosphatase in aleurone particles of rice grains.the major acid phosphatase (ec 3.1.3.2) associated with aleurone particles of rice grains (oryza sativa l. japonica cv. koshihikari) was purified to homogeneous state by polyacrylamide gel electrophoresis. its molecular weight was 72,000 when determined by gel filtration and 68,000 when found by polyacrylamide gradient gel electrophoresis in the presence of sodium dodecyl sulfate and β-mercaptoethanol. the purified enzyme had a violet color and an absorption peak at 530 nm. triton x-100 and lyso ...198025385961
polysomes and intracisternal accumulations in enucleate sieve elements of rice (oryza sativa l.).polysomes in sieve elements of rice (oryza sativa l.) were studied with the electron microscope. the polysomes were found on the rough endoplasmic reticulum (er) present in immature sieve elements and also on the cisternae of aggregated er in the parietal layer of mature, enucleate sieve elements. in the immature sieve elements the er cisternae existed as narrow profiles while in the mature sieve elements the er cisternae were considerably dilated and contained a fibrillar material and, occasion ...198024306690
potentiality of leaf sheath cells for regeneration of rice (oryza sativa l.) plants.regeneration of rice plantlets (oryza sativa l.) from calli originated from leaf sheath cells was made possible. this was possible in tissues initially grown in media containing 2.4-d (2.4-dichlorophenoxyacetic acid) at low temperature and illumination. the slow growing tissues were subsequently subjected to growth conditions at an elevated temperature and higher illumination with addition of kinetin and iaa and without 2.4-d. the suitability of leaf sheath cells for protoplast technology is ind ...198024301016
influence of streptomycin on alpha-amylase formation isolated endosperms & protein pattern in intact seedlings of rice oryza sativa l. 19806159319
effect of fluoride on in vivo nitrate reduction in the rive leaves (oryza sativa l.).fluoride had no effect on in vitro nitrate reductase activity in rice leaves, but in vivo activity was strongly inhibited. it is suggested that fluoride brings about this inhibition by adversely affecting the physiological nadh generating system required for in vivo nitrate reduction.19807191373
effects of toxic concentrations of nickel on growth & metabolism of rice (oryza sativa l.) seedlings. 19807399618
promotion of mesocotyl growth in etiolated rice seedlings by 4-ethoxy-1-(p-tolyl)-s-triazine-2,6(1h,3h)-dione.4-ethoxy-1-(p-tolyl)-s-triazine-2,6(1h,3h)-dione (ta) promoted mesocotyl growth in dark-grown rice (oryza sativa l.) seedlings. in cultivars of the japonica type ta alone showed a small promotive effect and ta+gibberellic acid(ga3) had a marked synergistic effect, while in other cultivars, mostly of the indica type, ta alone showed a great promotive effect and ta+ga3 had only an additive effect. in cv. nato, a typical representative of cultivars showing the second type of response, the concentra ...198024311174
effects of blue and red light on unrolling of rice leaves.unrolling of the second leaf of 8-day-old rice (oryza sativa l.) seedlings was promoted by weak blue light (b), but not by red light (r). the effect of b was counteracted by irradiation with r just before or after the b. the counteracting effect of r was reversed by subsequent irradiation with far-red light but not by b, even if b was applied for 10 h. the b was effective when the region 0.5-2 cm from the tip of the leaf was irradiated. these results indicate that in rice photoreceptors for blue ...198024311163
mechanism of monocarpic senescence in rice.during grain formation stage (90 to 110 days), the youngest flag leaf of rice (oryza sativa l. cv. jaya) remained metabolically most active (as indicated by cellular constituents and enzyme activities) and the third leaf the least active. at the grain development stage (110 to 120 days) the above pattern of age-related senescence of the flag leaf completely changed and it senesced at a faster rate than the second leaf which remained metabolically active even up to grain maturation time (120 to 1 ...198016661186
diallel analysis of heading date in rice (oryza sativa l.).the genetics of heading date was investigated in an 8×8 diallel set of crosses involving diverse rice cultivars. wr, vr graph analysis revealed the presence of a complementary type of non-allelic interaction which apparently affected the position and slope of the regression line such as if there were overdominance. omission of two interacting parents resulted in a 6×6 subset of diallel crosses from which, as observed in the wr, vr graph, the non-allelic interaction had disappeared and the regres ...198024302365
amino acid sequence of cytochrome c from rice.the amino acid sequence of cytochrome c purified from rice, oryza sativa l., was determined. the complete amino acid sequence of rice cytochrome c is as follows: ac-ala-8-ser-phe-ser-glu-ala-pro-pro-gly1-asn-pro-lys-ala-gly-glu-lys-ile-phe10-lys-thr-lys-cys-ala-glx-cys-his-thr-val20-asp-lys-gly-ala-gly-his-lys-glx-gly-pro30-asx-leu-asx-gly-leu-phe-gly-arg-glx-ser40-gly-thr-thr-pro-gly-tyr-ser-tyr-ser-thr50-ala-asp-lys-asn-met-ala-val-ile-trp-glx60-glx-asx-thr-leu-tyr-asp-tyr-leu-leu-asn70-pro-tm ...19806244261
penicillin-induced formation of ribonuclease in rice (oryza sativa l.) endosperm and its inhibition by abscisic acid.penicillin stimulates the formation of ribonuclease in embryoless rice (oryza sativa l.) endosperm and enhances gibberelin-induced response. penicillin-induced rnase production is completely inhibited by abscisic acid.1979227713
the major dna polymerase in cultured plant cells: partial purification and correlation with cell multiplication.a dna polymerase activity was isolated from cells of oryza sativa l. grown in suspension culture. molecular mass (∼ 180,000), optimal requirements for ph (neutral), mg(2+) (5-10 mm), mn(2+) (1 mm), template preference (activated dna), lack of activity with native or denatured dna, and sensitivity to n-ethylmaleimide and ionic strength are similar to those of the vertebrate α-polymerase. like dna polymerase α, the dna polymerase described in this work is the most abundant in proliferating cells o ...197924318322
mode of inheritance of dwarf stature and allelic relationships among various spontaneous and induced dwarfs of cultivated rice oryza sativa l.genetic study of spontaneous and induced dwarfs included the mode of inheritance of dwarf stature and the allelic relationships among various dwarfs. qualitative genetic analysis involving crosses of fourteen dwarfs with a common tall variety 'iari 11124' showed that the degree of dominance in the f1 hybrids varied with the cross. with the exception of the crosses of iari 6579 and iari 10560 with the tall variety, all crosses exhibited incomplete dominance. the segregation pattern in f2 populati ...197924306608
enzymic mechanisms of starch breakdown in germinating rice seeds: 7. amylase formation in the epithelium.the time sequence analysis of the starch digestion pattern of the thin sectioned germinating rice (oryza sativa l.) seed specimens using the starch film method showed that at the initial stage amylase activity was almost exclusively localized in the epithelium septum between the scutellum and endosperm. starch breakdown in the endosperm tissues began afterward; amylase activity in the aleurone layers was detectable only after 2 days. polyacrylamide gel electrofocusing (ph 4 to 6) revealed nearly ...197916660724
specificity of 1-triacontanol as a plant growth stimulator and inhibition of its effect by other long-chain compounds.the effect of several analogs of 1-triacontanol (tria), differing in c-chain length (16-32), the position of the hydroxyl group and the terminal functional group, were tested alone and in combination with tria on the growth of rice (oryza sativa l.), maize (zea mays l.) and tomato (lycopersicon esculentum mill.) seedlings. applied alone, none of the compounds caused an increase in growth; thus, chain length (30 c) and presence and position (terminal) of the hydroxyl group appear to be specific f ...197924407259
identification of gibberellins in the rice plant and quantitative changes of gibberellin a19 throughout its life cycle.the major endogenous gibberellin (ga) in shoots, roots and ears of the rice plant, oryza sativa l. japonica cv. nihonbare, was identified as ga19 by combined gas liquid chromatography-mass spectrometry (gc-ms) and gc-selected ion current monitoring (gc-sicm). another ga present in these tissues in small quantity was tentatively identified as ga1 by gc-sicm, and ga4 may be present in the seeds (kernels) of 3rd-leaf-stage seedlings. using gc-sicm, the ga19 content was quantified throughout the lif ...197924318057
endogenous plant growth substances in developing fruit of prunus cerasus l: vii. isolation of gibberellin a(32).a polar gibberellin active in the dwarf rice (oryza sativa l.) seedling and barley (hordeum vulgare l.) half-seed assays was extracted from immature sour cherry (prunus cerasus l.) seed and identified as gibberellin a(32) by gas-liquid chromatography and selected ion monitoring.197916660663
comparison of the uptake of nitrate and ammonium by rice seedlings: influences of light, temperature, oxygen concentration, exogenous sucrose, and metabolic inhibitors.the uptake of nitrate and ammonium by rice (oryza sativa) seedlings was compared under various conditions. nitrate uptake showed a 1-hour lag phase and then a rapid absorption phase, whereas ammonium uptake showed passive absorption during the first hour, then a shoulder of absorption, followed by a rapid metabolism-dependent absorption phase. light did not affect the uptake of nitrate or ammonium. the uptake of nitrate and ammonium was markedly suppressed by removal of the endosperm. after remo ...197816660579
studies on molybdenum absorption and transport in bean and rice.the patterns of molybdenum (moo(4) (2-)) absorption and transport were investigated in intact bean (phaseolus vulgaris l.) and rice (oryza sativa l. cv. i.r.8) plants. the mobility of moo(4) (2-) absorbed by roots and by leaves was compared with that of a freely mobile element, rb(+). although moo(4) (2-) absorption by bean roots was nearly as high as that of rb(+), its transport to the shoot was considerably less. when moo(4) (2-) was fed to one of the primary leaves, most of it was transported ...197816660481
environmental parameters affecting dark response of rice seedlings (oryza sativa l.) to triacontanol.triacontanol applied to ir-8 rice (oryza sativa l.) seedlings in nutrient solution caused an increase in dry weight during a 6-hour dark period. this increase was altered by atmospheric co(2) and o(2) concentrations. the largest growth response occurred from 200 to 350 muliters/liter co(2) with 5% o(2). the treated seedlings did not fix atmospheric co(2) in the dark, and the immediate products of photosynthesis were not involved in the dry weight increase. the growth response was characterized b ...197816660400
inheritance of semidwarfism in rice, oryza sativa l.the inheritance of plant height was investigated in a ten-parent diallel cross of diverse rice cultivars. parents included two tall japonica lines and eight semidwarf lines. data from parent, f(1), f(2 ), and f(3) generations indicated that the majority of height variation among the ten parents could be accounted for by three major genes with additive loci effects. d51, 72/2234-11, and g33 (derived from the known major-gene indica semidwarf dee-geo-woo-gen) all were found to possess an allelic, ...197817248812
biochemical basis of hybrid vigour. the genetics of grain weight of oryza sativa.four cathodal bands (c1, c2, c3 and c4) of esterase (e1, c1. 3.1) were correlated with the grain weight of rice (oryza sativa l.). zymogram patterns indicated intensity differences among these bands infinegrain and coarse-grain varieties. bands c1. and c2 were dark in fine grain varieties whereas c3 and c4 were dark in coarse grain varieties. these bands were specific to endosperm. observations on fine-grain (kalanamak), coarse grain (sr(26)b) varieties and their reciprocal hybrids indicated the ...197724407414
light-induced development of photosynthetic enzymes in etiolated detached leaves of oryza sativa and setaria italica during greening. 1977612539
in vitro development of plants from microspores of rice.rice (oryza sativa l., 2n=24) anthers containing microspores in the early-uninucleate to first-mitosis stages were induced successfully to develop into plants in vitro through an intermediary step of callus formation. callus initiation occurred with highest frequency in anthers containing mid-uninucleate imcrospores. the callus derived from different stages of microspore development differed in the potential to differentiate into plants. the plants regenerated from pollen callus were predominant ...1977903172
factors modifying radiation induced stimulation in plants: pre-irradiation seed moisture content.the effect of moisture content of seeds at the time of irradiation in relation to radiation-induced stimulation was investigated on rice (oryza sativa c.v. d-6-2-2). the optimum moisture content was 8% for stimulation measured as seedling height. it is concluded that seed moisture at the time of irradiation plays an important role in the expression of stimulation and its reproducibility.1977866584
free sugars in relation to starch accumulation in developing rice grain.the changes in sugars (water-soluble carbohydrates) were studied in the developing grain of rice (oryza sativa l., variety ir28 and ir29) in relation to the role of these sugars as precursors of adp glucose in starch accumulation. the levels of total sugars, total reducing sugars and free glucose, sucrose and other nonreducing sugars, maltooligosaccharides, and total and nonsucrosyl fructose followed closely the changes in the rate of starch accumulation, in both ir28 and 29; the peak value occu ...197716659864
some properties of 3-phosphoglycerate phosphatase from developing rice grain.some properties of 3-p-glycerate phosphatase from developing caryopsis of rice (oryza sativa l., variety ir26) were studied. the enzyme was found to be soluble and not bound to starch, and concentrated mainly in the pericarp-aleurone layer; its maximum activity was at 12 to 14 days after flowering. contents of 3-p-glycerate and chlorophyll were highest in the grain at 7 to 8 days after flowering when starch synthesis was at a maximum. the enzyme was purified about 100-fold by precipitation with ...197716659802
effect of fusicoccin on plant cell cultures and protoplasts.we have assayed the capacity of the fungal toxin fusicoccin to induce some of its characteristic effects (acidification of the medium, stimulation of k(+), and of 3-o-methyl-d-glucose uptake) in cell suspensions of parthenocissus tricuspidata (siebold et zucc.) planchon, acer pseudoplatanus l. and oryza sativa l., and in protoplast suspensions prepared from leaves of nicotiana tabacum l. and spinacia oleracea l. or from cultures of p. tricuspidata. evidence is presented showing that all tested b ...197724420024
growth responses of rice seedlings to triacontanol in light and dark.triacontanol, a 30-carbon primary alcohol, applied in nutrient culture solutions to rice (oryza sativa l.) seedlings at 2.3×10(-8) m (10 μg/l), caused an increase in dry weight and leaf area of the whole plants. the response could be observed as early as 3 h of treatment. it was observed at relatively high and low light intensities as well as in the dark where control plants lost but triacontanol-treated plants gained in dry weight. the dry weight gain in the dark was, however, eliminated by rem ...197724419896
nadh- and nad(p)h-nitrate reductases in rice seedlings.by use of affinity chromatography on blue dextran-sepharose, two nitrate reductases from rice (oryza sativa l.) seedlings, specifically, nadh:nitrate oxidoreductase (ec 1.6.6.1) and nad(p)-h:nitrate oxidoreductase (ec 1.6.6.2), have been partially separated. nitrate-induced seedlings contained more nadh-nitrate reductase than nad(p)h-nitrate reductase, whereas chloramphenicol-induced seedlings contained primarily nad(p)h-nitrate reductase. nad(p)h-nitrate reductase was shown to utilize nadph dir ...197616659665
aspects of nitrogen metabolism in the rice seedling.the effects of nitrogen source no(3) (-) or nh(4) (+) on nitrogen metabolism during the first 2 weeks of germination of the rice seedling (oryza sativa l., var. ir22) grown in nutrient solution containing 40 mug/ml n were studied. total, soluble protein, and free amino n levels were higher in the nh(4) (+)-grown seedling, particularly during the 1st week of germination. asparagine accounted for most of the difference in free amino acid level, in both the root and the shoot. nitrate and nitrite r ...197616659600
effects of ancymidol (a growth retardant) and triarimol (a fungicide) on the growth, sterols, and gibberellins of phaseolus vulgaris (l.).the effect of the two substituted pyrimidines, ancymidol (a growth retardant) and triarimol (a fungicide) on phaseolus vulgaris was studied. both compounds retarded shoot and root elongation as well as increases in fresh weight. both compounds caused production of ethylene-like responses when given in high dosages or when applied shortly after germination. as growth retardation was shown to occur in the absence of net increase in sterol levels, neither ancymidol nor triarimol apparently retards ...197616659542
quantitative genetically nonequivalent reciprocal crosses in cultivated plants.quantitative expressions of character difference between reciprocal crosses have been studied by different researchers in a number of plant species, such as epilobium, zea mays, oryza sativa, hordeum sativum, triticum aestivum, trifolium hybridum, linum usitatissimum, nicotiana rustica, and others. in all cases it was found that the nonequivalence of reciprocal crosses manifested itself beginning with the f1 generation, with the exception of some flax crosses in which reciprocals differed beginn ...20151032105
catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence.the activities of catalase, peroxidase, and polyphenoloxidase were studied in attached and detached rice (oryza sativa l. cv. ratna) leaves. catalase activity decreased while peroxidase and polyphenoloxidase activities increased during senescence of both attached and detached rice leaves. kinetic (5 mum) and benzimidazole (1 mm), which are known to delay the senescence of detached rice leaves, retarded the decrease of catalase activity during detached leaf senescence. on the other hand, these ch ...197616659474
responses of the rice shoot apex to irradiation with red and far-red light.determinations of cell-doubling times using the technique of colchicine-induced metaphase accumulation showed that after 40 h exposure to red or far-red light the rates of cell division in young rice (oryza sativa l. cv. ballila) shoot apices were faster than in dark controls. in red light, the increase was already taking place after 16 h of irradiation but in far-red the rates at this time were slower than in the dark controls. seedlings became more responsive to far-red light as they continued ...197624425084
enzymes of carbohydrate metabolism in the developing rice grain.the levels of reducing and nonreducing sugars, starch, soluble protein, and selected enzymes involved in the metabolism of sucrose, glucose-1-p, and glucose nucleotides were assayed in dehulled developing rice grains (oryza sativa l. line ir1541-76-3) during the first 3 weeks after flowering. the level of reducing sugars in the grain was highest 5 to 6 days after flowering. the level of nonreducing sugars and the rate of starch accumulation were maximum 11 to 12 days after flowering, when the le ...197516659348
staining of sieve tube mitochondria in coleoptiles of rice (oryza sativa l.) with diaminobenzidine.diaminobenzidine has been used to locate sites of oxidizing cytochromes in rice coleoptiles. the mitochondria of mature sieve tubes show a positive reaction which to visual inspection appears as strong as the reaction of companion cell mitochondria.197524435994
correlation between the age related metabolites and photoinduction in oryza sativa. 19744442472
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