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a protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (oryza sativa l.).nitric oxide (no) and salicylic acid (sa) are important signaling molecules in plant system. in the present study both no and sa showed a protective role against arsenite (asiii) stress in rice plants when supplied exogenously. the application of no and sa alleviated the negative impact of asiii on plant growth. nitric oxide supplementation to asiii treated plants greatly decreased arsenic (as) accumulation in the roots as well as shoots/roots translocation factor. arsenite exposure in plants de ...201728371690
genetic enhancer analysis reveals that floral organ number2 and osmads3 co-operatively regulate maintenance and determinacy of the flower meristem in rice.meristems such as the shoot apical meristem and flower meristem (fm) act as a reservoir of stem cells, which reproduce themselves and supply daughter cells for the differentiation of lateral organs. in oryza sativa (rice), the floral organ number2 (fon2) gene, which is similar to arabidopsis clavata3, is involved in meristem maintenance. in fon2 mutants, the numbers of floral organs are increased due to an enlargement of the fm. to identify new factors regulating meristem maintenance in rice, we ...201728371923
development and genetic characterization of a novel herbicide (imazethapyr) tolerant mutant in rice (oryza sativa l.).increased water and labour scarcity in major rice growing areas warrants a shift towards direct seeded rice cultivation under which management of weeds is a major issue. use of broad spectrum non-selective herbicides is an efficient means to manage weeds. availability of rice genotypes with complete tolerance against broad-spectrum non-selective herbicides is a pre-requisite for advocating use of such herbicides. in the present study, we developed an ems induced rice mutant, 'htm-n22', exhibitin ...201728378144
rice no pollen 1 (np1) is required for anther cuticle formation and pollen exine patterning.angiosperm male reproductive organs (anthers and pollen grains) have complex and interesting morphological features, but mechanisms that underlie their patterning are poorly understood. here we report the isolation and characterization of a male sterile mutant of no pollen 1 (np1) in rice (oryza sativa). the np1-4 mutant exhibited smaller anthers with a smooth cuticle surface, abnormal ubisch bodies, and aborted pollen grains covered with irregular exine. wild-type exine has two continuous layer ...201728378445
overexpression of an arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field.drought stress has often caused significant decreases in crop production which could be associated with global warming. enhancing drought tolerance without a grain yield penalty has been a great challenge in crop improvement. here, we report the arabidopsis thaliana galactinol synthase 2 gene (atgols2) was able to confer drought tolerance and increase grain yield in two different rice (oryza sativa) genotypes under dry field conditions. the developed ubi:atgols2 transgenic lines also had higher ...201728378532
rice fatty acyl-coa synthetase osacos12 is required for tapetum programmed cell death and male fertility.loss of function mutation of rice osacos12 impairs lipid metabolism-mediated anther cuticle and pollen wall formation, and interferes with tapetum programmed cell death, leading to male sterility. acyl-coa synthetase (acos) is one of the enzymes activating fatty acids for various metabolic functions in plants. here, we show that osacos12, an orthologue of arabidopsis acos5 in rice, is crucial for rice fertility. similar to acos5, osaocs12 mutant had no mature pollen. but unlike acos5, osaocs12 p ...201728382520
a phosphoproteomic landscape of rice (oryza sativa) tissues.protein phosphorylation is an important post-translational modification that regulates various plant developmental processes. here, we report a comprehensive, quantitative phosphoproteomic profile of six rice tissues, including callus, leaf, root, shoot meristem, young panicle and mature panicle from nipponbare by employing a ms-based, label-free approach. a total of 7171 unique phosphorylation sites in 4792 phosphopeptides from 2657 phosphoproteins were identified, including 4613 peptides were ...201728382632
identified of a novel cis-element regulating the alternative splicing of lcdreb2.alternative splicing (as) is an important gene regulation mechanism in plants. despite the widespread use of as in plant gene expression regulation, the identification of the cis-elements involved in the as mechanism is rarely reported in plants. to explore the regulation mechanism of the as of lcdreb2, a dreb2 ortholog from sheepgrass (leymus chinensis), the genomic sequences of lcdreb2 and its homologs in poaceae were aligned, and six mutations were introduced in the conserved sequence of lcdr ...201728383047
oslg3 contributing to rice grain length and yield was mined by ho-lamap.most agronomic traits in rice are complex and polygenic. the identification of quantitative trait loci (qtl) for grain length is an important objective of rice genetic research and breeding programs.201728385155
disrupting flavone synthase ii alters lignin and improves biomass digestibility.lignin, a ubiquitous phenylpropanoid polymer in vascular plant cell walls, is derived primarily from oxidative couplings of monolignols (p-hydroxycinnamyl alcohols). it was discovered recently that a wide range of grasses, including cereals, utilize a member of the flavonoids, tricin (3',5'-dimethoxyflavone), as a natural comonomer with monolignols for cell wall lignification. previously, we established that cytochrome p450 93g1 is a flavone synthase ii (osfnsii) indispensable for the biosynthes ...028385728
molecular, structural, and phylogenetic analyses of taxus chinensis jazs.taxus spp. are ancient gymnosperms that produce a unique secondary metabolite, namely, taxol, an anticancer drug. jaz proteins are key regulators of the ja signaling pathway, which control taxol biosynthesis. however, the jaz proteins of taxus spp. are poorly studied. in this work, nine jaz genes from taxus chinensis were identified using our previous transcriptome data and named as tcjaz1-tcjaz9. of these nine tcjaz proteins, eight contain jas and tify domains, and the jas domain of tcjaz6 is i ...201728390989
gw5 acts in the brassinosteroid signalling pathway to regulate grain width and weight in rice.grain size is a major determinant of grain yield in cereal crops. qsw5/gw5, which exerts the greatest effect on rice grain width and weight, was fine-mapped to a 2,263-bp/21-kb genomic region containing a 1,212-bp deletion, respectively. here, we show that a gene encoding a calmodulin binding protein, located ∼5 kb downstream of the 1,212-bp deletion, corresponds to qsw5/gw5. gw5 is expressed in various rice organs, with highest expression level detected in young panicles. we provide evidence th ...201728394310
effects of microwaves on the reduction of aspergillus flavus and aspergillus parasiticus on brown rice (oryza sativa l.) and barley (hordeum vulgare l.).aspergillus flavus and aspergillus parasiticus are primary pathogen moulds on brown rice and barley. this study investigated the effects of microwave irradiation (mwi) (2450 mhz, 700 w, 10-50 s) on inactivation of a. flavus and a. parasiticus on brown rice and barley and the quality of these samples. the counts of both strains were significantly (p < 0.05) reduced by the stepwise increase in mwi treatment time. the log reductions of a. flavus on brown rice and barley were 0.05 and 0.04 after 10 ...201728399711
a virus-induced assay for functional dissection and analysis of monocot and dicot flowering time genes.virus-induced flowering (vif) uses virus vectors to express flowering locus t (ft) to induce flowering in plants. this approach has recently attracted wide interest for its practical applications in accelerating breeding in crops and woody fruit-trees. however, the insight into vif and its potential as a powerful tool for dissecting florigenic proteins remained to be elucidated. here, we describe the mechanism and further applications of potato virus x (pvx)-based vif in the short-day nicotinana ...201728400493
genome-wide identification and characterization of nb-arc resistant genes in wheat (triticum aestivum l.) and their expression during leaf rust infection.nb-arc domain-containing resistance genes from the wheat genome were identified, characterized and localized on chromosome arms that displayed differential yet positive response during incompatible and compatible leaf rust interactions. wheat (triticum aestivum l.) is an important cereal crop; however, its production is affected severely by numerous diseases including rusts. an efficient, cost-effective and ecologically viable approach to control pathogens is through host resistance. in wheat, h ...201728401336
a secreted chitinase-like protein (osclp) supports root growth through calcium signaling in oryza sativa.chitinases belong to a conserved protein family and play multiple roles in defense, development and growth regulation in plants. here, we identified a secreted chitinase-like protein, osclp, which functions in rice growth. a t-dna insertion mutant of osclp (osclp) showed significant retardation of root and shoot growth. a comparative proteomic analysis was carried out using root tissue of wild-type and the osclp mutant to understand the osclp-mediated rice growth retardation. results obtained re ...201728401568
regulation of strigolactone biosynthesis by gibberellin signaling.strigolactones (sls) are a class of plant hormones that regulate diverse physiological processes, including shoot branching and root development. they also act as rhizosphere signaling molecules to stimulate the germination of root parasitic weeds and the branching of arbuscular mycorrhizal fungi. although various types of cross talk between sls and other hormones have been reported in physiological analyses, the cross talk between gibberellin (ga) and sls is poorly understood. we screened for c ...201728404726
comparative analysis of expression profiles of panicle development among tolerant and sensitive rice in response to drought stress.water deficit caused a serious threat to crops, especially panicle development at reproductive growth phase. we investigated grain yield components and gene expression profiles of panicle among tolerant and sensitive rice in response to drought stress. panicle morphologies exhibited that secondary branches per panicle were more severely affected as compared to primary branches per panicle. moreover, grain weight per panicle showed significant decrease for both tolerant and sensitive varieties ex ...201728405199
lethal albinic seedling, encoding a threonyl-trna synthetase, is involved in development of plastid protein synthesis system in rice.an albinic rice is caused by mutation of threonyl-trna synthetase, which is essential for plant development by stabilizing of nep and pep gene expressions and chloroplast protein synthesis. chloroplast biogenesis and development depend on complex genetic mechanisms. apart from their function in translation, aminoacyl-trna synthetases (aarss) play additional role in gene expression regulation, rna splicing, and cytokine activity. however, their detailed functions in plant development are still po ...201728405745
genome-wide changes in microrna expression during short and prolonged heat stress and recovery in contrasting rice cultivars.micrornas (mirnas) are known to regulate expression of genes under stress. we report here the deep sequencing of small rnas expressed during control, short and prolonged heat stress and recovery. genome-wide identification of mirnas in tolerant (nagina 22) and susceptible (vandana) rice cultivars was performed in 16 samples representing root and shoot of 13-day-old seedlings. the expression profile of mirnas was analysed in 36 pairwise combinations to identify the genotype-, treatment- and tissu ...201728407080
oshac4 is critical for arsenate tolerance and regulates arsenic accumulation in rice.soil contamination with arsenic (as) can cause phytotoxicity and elevated as accumulation in rice grain. here, we used a forward genetics approach to investigate the mechanism of arsenate (as(v)) tolerance and accumulation in rice. a rice mutant hypersensitive to as(v), but not to as(iii), was isolated. genomic resequencing and complementation tests were used to identify the causal gene. the function of the gene, its expression pattern and subcellular localization were characterized. oshac4 is t ...201728407265
brachytic 1 of barley (hordeum vulgare l.) encodes the α subunit of heterotrimeric g protein.physiological and molecular biological analysis of the dwarf barley (hordeum vulgare l.) mutant brachytic 1 (brh1) was conducted. the root responses of brh1 to brassinolide were weaker than those of wild type, but the responses of leaf segments of dark-grown plants were not. responses of brh1 to gibberellin a3 were similar to or slightly stronger than those of wild type. endogenous levels of these hormones in young seedlings were not clearly different between brh1 and wild type. skotomorphogenes ...201728412605
disulfide proteomics of rice cultured cells in response to osracl and probenazole-related immune signaling pathway in rice.reactive oxygen species (ros) production is an early event in the immune response of plants. ros production affects the redox-based modification of cysteine residues in redox proteins, which contribute to protein functions such as enzymatic activity, protein-protein interactions, oligomerization, and intracellular localization. thus, the sensitivity of cysteine residues to changes in the cellular redox status is critical to the immune response of plants.201628413359
genome-wide analysis of vq motif-containing proteins in moso bamboo (phyllostachys edulis).29 moso bamboo vq proteins were genome-wide identified for the first time, and bioinformatics analysis was performed to investigate phylogenetic relationships and evolutionary divergence. the qrt-pcr data show that pevq genes response to different stress treatments. accumulating evidence suggests that vq motif-containing proteins in rice (oryza sativa), arabidopsis (arabidopsis thaliana), and maize (zea mays) play fundamental roles in response to various biotic and abiotic stresses. however, lit ...201728417193
selective sweep with significant positive selection serves as the driving force for the differentiation of japonica and indica rice cultivars.asian cultivated rice (oryza sativa l.), including japonica and indica, is unarguable the most important crop in asia as well as worldwide. however, a decisive conclusion of its origination and domestication processes are still lacking. nowadays, the ever-increasing high-throughput sequencing data of numerous rice samples have provided us new opportunities to get close to the answer of these questions.201728420345
regulation of coniferaldehyde 5-hydroxylase expression to modulate cell wall lignin structure in rice.regulation of a gene encoding coniferaldehyde 5-hydroxylase leads to substantial alterations in lignin structure in rice cell walls, identifying a promising genetic engineering target for improving grass biomass utilization. the aromatic composition of lignin greatly affects utilization characteristics of lignocellulosic biomass and, therefore, has been one of the primary targets of cell wall engineering studies. limited information is, however, available regarding lignin modifications in monoco ...201728421330
distribution, genetic diversity and potential spatiotemporal scale of alien gene flow in crop wild relatives of rice (oryza spp.) in colombia.crop wild relatives (cwrs) of rice hold important traits that can contribute to enhancing the ability of cultivated rice (oryza sativa and o. glaberrima) to produce higher yields, cope with the effects of climate change, and resist attacks of pests and diseases, among others. however, the genetic resources of these species remain dramatically understudied, putting at risk their future availability from in situ and ex situ sources. here we assess the distribution of genetic diversity of the four ...201728421550
qtl analysis and dissection of panicle components in rice using advanced backcross populations derived from oryza sativa cultivars hr1128 and 'nipponbare'.panicle traits are among the most important agronomic characters which directly relate to yield in rice. grain number (gn), panicle length (pl), primary branch number (pbn), and secondary branch number (sbn) are the major components of rice panicle structure, and are all controlled by quantitative trait loci (qtls). in our research, four advanced backcross overlapping populations (bil152, bil196a, bil196b, and bil196b-156) carrying introgressed segments from chromosome 6 were derived from an ind ...201728422981
evolutionary analysis of snf1-related protein kinase2 (snrk2) and calcium sensor (scs) gene lineages, and dimerization of rice homologs, suggest deep biochemical conservation across angiosperms.members of the sucrose non-fermenting related kinase group2 (snrk2) subclasses are implicated in both direct and indirect abscisic acid (aba) response pathways. we have used phylogenetic, biochemical, and transient in vivo approaches to examine interactions between triticum tauschii protein kinase 1 (ttpk1) and an interacting protein, oryza sativa snrk2-calcium sensor (osscs1). given that ttpk1 has 100% identity with its rice ortholog, osmotic stress/aba-activated protein kinase (ossapk2), we hy ...201728424709
non-essential element concentrations in brown grain rice: assessment by advanced data mining techniques.the concentrations of 17 non-essential elements (al, as, ba, be, cd, ce, cr, hg, la, li, pb, sb, sn, sr, th, ti, and tl) were determined in brown grain rice samples of two varieties: fortuna and largo fino. the samples were collected from the four main producing regions of corrientes province (argentina). quantitative determinations were performed by inductively coupled plasma mass spectrometry (icp-ms), using a validated method. the contents of as, be, cd, ce, cr, hg, pb, sb, sn, th, and tl wer ...201728424959
manipulation of glyoxalase pathway confers tolerance to multiple stresses in rice.crop plants face a multitude of diverse abiotic and biotic stresses in the farmers' fields. although there now exists a considerable knowledge of the underlying mechanisms of response to individual stresses, the crosstalk between response pathways to various abiotic and biotic stresses remains enigmatic. here, we investigated if the cytotoxic metabolite methylglyoxal (mg), excess of which is generated as a common consequence of many abiotic and biotic stresses, may serve as a key molecule linkin ...201728425127
fine mapping of a novel defective glume 1 (dg1) mutant, which affects vegetative and spikelet development in rice.in cereal crops, vegetative and spikelet development play important roles in grain yield and quality, but the genetic mechanisms that control vegetative and spikelet development remain poorly understood in rice. here, we identified a new rice mutant, defective glume 1 (dg1) mutant from cultivar zhonghua11 after ethyl methanesulfonate treatment. the dg1 mutant displayed the dwarfism with small, rolled leaves, which resulted from smaller cells and more bulliform cells. the dg1 mutant also had an e ...201728428794
diurnal changes in transcript and metabolite levels during the iron deficiency response of rice.rice (oryza sativa l.) is highly susceptible to iron (fe) deficiency due to low secretion levels of the mugineic acid (ma) family phytosiderophore (ps) 2'-deoxymugineic acid (dma) into the rhizosphere. the low levels of dma secreted by rice have proved challenging to measure and, therefore, the pattern of dma secretion under fe deficiency has been less extensively studied relative to other graminaceous monocot species that secrete high levels of ps, such as barley (hordeum vulgare l.).201728429296
identification of qtls for yield and agronomic traits in rice under stagnant flooding conditions.stagnant flooding, where water of 25-50 cm remains until harvest time, is a major problem in rainfed lowland areas. most of the sub1 varieties, which can withstand around 2 weeks of complete submergence, perform poorly in these conditions. hence, varieties tolerant of stagnant flooding are essential.201728429297
digestion property and synergistic effect on biological activity of purple rice (oryza sativa l.) anthocyanins subjected to a simulated gastrointestinal digestion in vitro.anthocyanins, a group of polyphenolic pigments, have been proved to possess various bioactivities. however, they are unstable in the small intestine and absorbed with low bioavailability. the discrepancy between the low bioavailability of anthocyanins and their good bioactivities has not been illuminated yet. moreover, information about the digested property and stability of purple rice anthocyanins in the alimentary tract is still limited. thus, the present work was designed to study the digest ...201528433272
genome-wide association studies to identify rice salt-tolerance markers.salinity is an ever increasing menace that affects agriculture worldwide. crops such as rice are salt sensitive, but its degree of susceptibility varies widely between cultivars pointing to extensive genetic diversity that can be exploited to identify genes and proteins that are relevant in the response of rice to salt stress. we used a diversity panel of 306 rice accessions and collected phenotypic data after short (6 h), medium (7 d) and long (30 d) salinity treatment (50 mm nacl). a genome-wi ...201728436093
a quantitative proteomic analysis of brassinosteroid-induced protein phosphorylation in rice (oryza sativa l.).the group of polyhydroxysteroid phytohormones referred to as the brassinosteroids (brs) is known to act on plant development and the stress response. br signal transduction relies largely on protein phosphorylation. by employing a label-free, ms (mass spectrometry)-based phosphoproteomic approach, we report here the largest profiling of 4,034 phosphosites on 1,900 phosphoproteins from rice young seedlings and their dynamic response to br. 1,821 proteins, including kinases, transcription factors ...201728439285
side-effects of pesticides used in irrigated rice areas on telenomus podisi ashmead (hymenoptera: platygastridae).telenomus podisi ashmead (hymenoptera: platygastridae) is an important agent for the biological control of stink bug eggs in irrigated rice areas and the best strategy for its preservation is the use of selective pesticides. the aim of this study was to know the side-effects of pesticides used in brazilian irrigated rice areas on egg parasitoid t. podisi. we evaluated, under laboratory conditions, 13 insecticides, 11 fungicides, 11 herbicides, and a control (distilled water) in choice and no-cho ...201728439681
qtls and analysis of the candidate gene for amylose, protein, and moisture content in rice (oryza sativa l.).in this study, we determined using nirs the heritability percentage of amylose, protein, and moisture content in polished and unpolished rice in a cndh population derived from a cross between cheongcheong and nagdong rice varieties. the results revealed a higher heritability percentage for the amylose content and compromised heritability for protein and moisture contents. we also conducted qtl analysis of rice for these major components and identified their chromosomal locations on a physical ma ...201728439810
becoming weeds.a new resequencing analysis of weedy rice (oryza sativa l.) biotypes illuminates distinct evolutionary paths and outcomes of de-domestication and ferality. this largest effort to date in weedy plant genomics gives a better understanding of weediness while also providing a promising source of alleles for rice breeding.201728442795
novel alleles of phosphorus-starvation tolerance 1 gene (pstol1) from oryza rufipogon confers high phosphorus uptake efficiency.limited phosphorus availability in the soil is one of the major constraints to the growth and productivity of rice across asian, african and south american countries, where 50% of the rice is grown under rain-fed systems on poor and problematic soils. with an aim to determine novel alleles for enhanced phosphorus uptake efficiency in wild species germplasm of rice oryza rufipogon, we investigated phosphorus uptake1 (pup1) locus with 11 previously reported ssr markers and sequence characterized t ...201728443109
three-dimensional intracellular structure of a whole rice mesophyll cell observed with fib-sem.ultrathin sections of rice leaf blades observed two-dimensionally using a transmission electron microscope (tem) show that the chlorenchyma is composed of lobed mesophyll cells, with intricate cell boundaries, and lined with chloroplasts. the lobed cell shape and chloroplast positioning are believed to enhance the area available for the gas exchange surface for photosynthesis in rice leaves. however, a cell image revealing the three-dimensional (3-d) ultrastructure of rice mesophyll cells has no ...201728444369
cloning and characterization of auxin efflux carrier genes ecpin1a and ecpin1b from finger millet eleusine coracana l.auxin signaling events in plants play important role in developmental regulation as well as gravitropic responses and plays crucial role in the development of root, lateral root and root hairs. the gene that is known to be most important in the development of root, lateral root and root hairs is commonly known as auxin efflux carrier (pin). being commonly known as orphan plant, the genome sequence of eleusine coracana is not known yet, and hence it was very difficult to conduct advanced research ...201728444595
difference in oxidative stress tolerance between rice cultivars estimated with chlorophyll fluorescence analysis.oxidative stress is considered to be involved in growth retardation of plants when they are exposed to a variety of biotic and abiotic stresses. despite its potential importance in improving crop production, comparative studies on oxidative stress tolerance between rice (oryza sativa l.) cultivars are limited. this work describes the difference in term of oxidative stress tolerance between 72 rice cultivars.201728446247
overexpression of mir529a confers enhanced resistance to oxidative stress in rice (oryza sativa l.).overexpressing mir529a can enhance oxidative stress resistance by targeting osspl2 and osspl14 genes that can regulate the expression of their downstream sod and pod related genes. micrornas are involved in the regulation of plant developmental and physiological processes, and their expression can be altered when plants suffered environment stresses, including salt, oxidative, drought and cadmium. the expression of microrna529 (mir529) can be induced under oxidative stress. however, its biologic ...201728451819
nitrogen fertilizer application affects lodging resistance by altering secondary cell wall synthesis in japonica rice (oryza sativa).stem mechanical strength is an important agricultural quantitative trait that is closely related to lodging resistance in rice, which is known to be reduced by fertilizer with higher levels of nitrogen. to understand the mechanism that regulates stem mechanical strength in response to nitrogen, we analysed stem morphology, anatomy, mechanical properties, cell wall components, and expression of cell wall-related genes, in two varieties of japonica rice, namely, wuyunjing23 (lodging-resistant vari ...201728451936
uptake and translocation of imidacloprid, thiamethoxam and difenoconazole in rice plants.uptake and translocation of imidacloprid (imi), thiamethoxam (thx) and difenoconazole (dfz) in rice plants (oryza sativa l.) were investigated with a soil-treated experiment at two application rates: field rate (fr) and 10*fr under laboratory conditions. the dissipation of the three compounds in soil followed the first-order kinetics and dfz showed greater half-lives than imi and thx. detection of the three compounds in rice tissues indicated that rice plants could take up and accumulate these p ...201728454637
effects of fertilization on crop production and nutrient-supplying capacity under rice-oilseed rape rotation system.incredible accomplishments have been achieved in agricultural production in china, but many demanding challenges for ensuring food security and environmental sustainability remain. field experiments were conducted from 2011-2013 at three different sites, including honghu, shayang, and jingzhou in china, to determine the effects of fertilization on enhancing crop productivity and indigenous nutrient-supplying capacity (inus) in a rice (oryza sativa l.)-rapeseed (brassica napus l.) rotation. four ...201728455510
genome-wide analysis of rice dehydrin gene family: its evolutionary conservedness and expression pattern in response to peg induced dehydration stress.abiotic stresses adversely affect cellular homeostasis, impairing overall growth and development of plants. these initial stress signals activate downstream signalling processes, which, subsequently, activate stress-responsive mechanisms to re-establish homeostasis. dehydrins (dhns) play an important role in combating dehydration stress. rice (oryza sativa l.), which is a paddy crop, is susceptible to drought stress. as drought survival in rice might be viewed as a trait with strong evolutionary ...201728459834
mechanism study of sulfur fertilization mediating copper translocation and biotransformation in rice (oryza sativa l.) plants.metabolism of sulfur (s) is suggested to be an important factor for the homeostasis and detoxification of cu in plants. we investigated the effects of s fertilizers (s0, na2so4) on cu translocation and biotransformation in rice plants by using multiple synchrotron-based techniques. fertilization of s increased the biomass and yield of rice plants, as well as the translocation factor of cu from root to shoot and shoot to grain, resulting in enhanced cu in grain. sulfur k-edge x-ray near edge stru ...201728461082
exogenous application of rutin and gallic acid regulate antioxidants and alleviate reactive oxygen generation in oryza sativa l.the effect of rutin and gallic acid on growth, phytochemical and defense gene activation of rice (oryza sativa l.) was investigated. the seeds of rice were primed with different concentrations of rutin and gallic acid (10-60 µg ml-1) to explicate the effect on germination on water agar plates. further, to study the effect of most effective concentrations of gallic acid (60 µg ml-1) and rutin (50 µg ml-1), greenhouse pot experiment was set up to determine the changes in growth, antioxidant and de ...201728461719
ethylene-inhibited jasmonic acid biosynthesis promotes mesocotyl/coleoptile elongation of etiolated rice seedlings.elongation of the mesocotyl and coleoptile facilitates the emergence of rice (oryza sativa) seedlings from soil and is affected by various genetic and environment factors. the regulatory mechanism underlying this process remains largely unclear. here, we examined the regulation of mesocotyl and coleoptile growth by characterizing a gaoyao1 (gy1) mutant that exhibits a longer mesocotyl and longer coleoptile than its original variety of rice. gy1 was identified through map-based cloning and encode ...201728465411
an osmotin from the resurrection plant tripogon loliiformis (tlosm) confers tolerance to multiple abiotic stresses in transgenic rice.osmotin is a key protein associated with abiotic and biotic stress response in plants. in this study, an osmotin from the resurrection plant tripogon loliiformis (tlosm) was characterized and functionally analyzed under abiotic stress conditions in t. loliiformis as well as in transgenic nicotiana tabacum (tobacco) and oryza sativa (rice) plants. real-time pcr analysis on mixed elicitor cdna libraries from t. loliiformis showed that tlosm was upregulated a 1000-fold during the early stages of os ...201828466470
seqping: gene prediction pipeline for plant genomes using self-training gene models and transcriptomic data.gene prediction is one of the most important steps in the genome annotation process. a large number of software tools and pipelines developed by various computing techniques are available for gene prediction. however, these systems have yet to accurately predict all or even most of the protein-coding regions. furthermore, none of the currently available gene-finders has a universal hidden markov model (hmm) that can perform gene prediction for all organisms equally well in an automatic fashion.201728466793
suppressive effect of microrna319 expression on rice plant height.key message: mir319 was identified as a dwarf-inducing gene from shiokari and its dwarf near isogenic line, and its transgenic rice showed a reduced plant height. this finding reveals the potential application of mir319 in future molecular breeding. it is well known that micrornas (mirnas) play important roles in plant physiology, especially in development and stress responses. however, little is known about the role of mirnas in plant height. in this study, the rice cultivar shiokari and its dw ...201728470512
the first plant acyl-coa-binding protein structures: the close homologues osacbp1 and osacbp2 from rice.acyl-coa-binding proteins (acbps) are a family of proteins that facilitate the binding of long-chain acyl-coa esters at a conserved acyl-coa-binding domain. acbps act to form intracellular acyl-coa pools, transport acyl-coa esters and regulate lipid metabolism. in the model plant arabidopsis thaliana, a family of six acbps has been demonstrated to function in stress and development. six acbps (osacbps) have also been identified in oryza sativa (rice), but they are not as well characterized as th ...201728471368
evaporation kinetics of surfactant solution droplets on rice (oryza sativa) leaves.the dynamics of evaporating sessile droplets on hydrophilic or hydrophobic surfaces is widely studied, and many models for these processes have been developed based on experimental evidence. however, few research has been explored on the evaporation of sessile droplets of surfactant or pesticide solutions on target crop leaves. thus, in this paper the impact of surfactant concentrations on contact angle, contact diameter, droplet height, and evolution of the droplets' evaporative volume on rice ...201728472108
l-cysteine desulfhydrase-related h2 s production is involved in osse5-promoted ammonium tolerance in roots of oryza sativa.previous studies revealed that rice heme oxygenase photoperiod sensitivity 5 (osse5) is involved in the regulation of tolerance to excess ammonium by enhancing antioxidant defence. in this study, the relationship between osse5 and hydrogen sulfide (h2 s), a well-known signalling molecule, was investigated. results showed that nh4 cl triggered the induction of l-cysteine desulfhydrase (l-des)-related h2 s production in rice seedling roots. a h2 s donor not only alleviated the excess ammonium-trig ...201728474399
salinity affects metabolomic profiles of different trophic levels in a food chain.salinization is one of the most important abiotic stressors in an ecosystem. to examine how exposing a host plant to excess salt affects the consequent performance and metabolism of insects in a food chain, we determined the life history traits and the metabolite profiles in rice (oryza sativa), the herbivore sitobion avenae, and its predator harmonia axyridis. when compared with performance under normal (non-stressed) conditions, exposing plants to 50mm nacl significantly delayed the timing of ...028475913
inhibitory effect of tio2 nps on symbiotic arbuscular mycorrhizal fungi in plant roots.while nanoparticles (nps) are known to exhibit antimicrobial properties, their effects on symbiotic arbuscular mycorrhizal fungi (amf) in plant roots has to be carefully examined as nps particularly of titanium dioxide (tio2) reach plant roots through varied sources such as fertilisers, plant protection products and other nanoproducts. the objective of the present study is to assess the effect of tio2 nps on the symbiotic behaviour of amf colonising rice (oryza sativa l.) plants. using sol-gel m ...201728476964
size dependent effects of antifungal phytogenic silver nanoparticles on germination, growth and biochemical parameters of rice (oryza sativa l), maize (zea mays l) and peanut (arachis hypogaea l).advancement in materials synthesis largely depends up on their diverse applications and commercialisation. antifungal effects of phytogenic silver nanoparticles (agnps) were evident, but the reports on the effects of the same on agricultural crops are scant. herein, we report for the first time, size dependent effects of phytogenic agnps (synthesised using stevia rebaudiana leaf extract) on the germination, growth and biochemical parameters of three important agricultural crops viz., rice (oryza ...201728476985
effect of microorganisms on reducing cadmium uptake and toxicity in rice (oryza sativa l.).this study analyzed the application of three microorganism inoculums, including bacillus subtilis, bacillus cereus, and commercial effective microorganism (em) solution in order to determine cadmium (cd) reduction in rice (oryza sativa l.) and rice growth promotion. rice was grown in cd-contaminated soil (120 mg/kg) and selected microorganisms were inoculated. cd concentration and rice weight were measured at 45 and 120 days of the experiment. the result showed that b. subtilis inoculation into ...201728480489
metabolomic analysis of the effects of cadmium and copper treatment in oryza sativa l. using untargeted liquid chromatography coupled to high resolution mass spectrometry and all-ion fragmentation.while the knowledge of plant metabolomes has increased in the last few years, their response to the presence of toxicants is still poorly understood. here, we analyse the metabolomic changes in japanese rice (oryza sativa var. japonica) upon exposure to heavy metals (cd(ii) and cu(ii)) in concentrations from 10 to 1000 μm. after harvesting, rice metabolites were extracted from aerial parts of the plants and analysed by hplc (hilic tsk gel amide-80 column) coupled to a mass spectrometer quadrupol ...201728480907
stable mercury isotopes in polished rice (oryza sativa l.) and hair from rice consumers.mercury (hg) isotopic signatures were characterized in polished rice samples from china, u.s., and indonesia (n = 45). hg isotopes were also analyzed in paired hair samples for participants from china (n = 21). for the latter, we also quantified the proportion of methylmercury intake through rice (range: 31-100%), and the weekly servings of fish meals (range: 0-5.6 servings/weekly). for these participants, 29% (n = 6) never ingested fish, 52% (n = 11) ingested fish < twice/weekly, and 19% (n = 4 ...201728482656
the rice tcd11 encoding plastid ribosomal protein s6 is essential for chloroplast development at low temperature.plastid ribosome proteins (prps) are important components for chloroplast biogenesis and early chloroplast development. although it has been known that chloroplast ribosomes are similar to bacterial ones, the precise molecular function of ribosomal proteins remains to be elucidated in rice. here, we identified a novel rice mutant, designated tcd11 (thermo-sensitive chlorophyll-deficient mutant 11), characterized by the albino phenotype until it died at 20°c, while displaying normal phenotype at ...201728483049
a novel class i chitinase from hippophae rhamnoides: indications for participating in ice-cbf cold stress signaling pathway.plant chitinases are the members of pr (pathogenesis related) proteins family and protect plants from biotic and abiotic stress. a novel chitinase hrchi1 (accession number jq289153) of 954bp orf encoding 317 amino acids protein was cloned, expressed and characterized from seabuckthorn, a cold/freeze tolerant shrub. the 3d structure (predicted with i-tasser server) showed highest homology with oryza sativa class i chitinase (pdb 2dkva). putative promoter region (obtained by genome walking) showed ...201728483054
processes underlying a reproductive barrier in indica-japonica rice hybrids revealed by transcriptome analysis.in rice (oryza sativa), hybrids between indica and japonica subspecies are usually highly sterile, which provides a model system for studying postzygotic reproductive isolation. a killer-protector system, s5, composed of three adjacent genes (orf3, orf4, and orf5), regulates female gamete fertility of indica-japonica hybrids. to characterize the processes underlying this system, we performed transcriptomic analyses of pistils from rice variety balilla (bl), balilla with transformed orf5+ (bl5+) ...201728483876
ancient plant glyoxylate/succinic semialdehyde reductases: glyr1s are cytosolic, whereas glyr2s are localized to both mitochondria and plastids.plant nadph-dependent glyoxylate/succinic semialdehyde reductases 1 and 2 (glyr1 and glyr2) are considered to be involved in detoxifying harmful aldehydes, thereby preserving plant health during exposure to various abiotic stresses. phylogenetic analysis revealed that the two glyr isoforms appeared in the plant lineage prior to the divergence of the chlorophyta and streptophyta, which occurred approximately 750 million years ago. green fluorescent protein fusions of apple (malus x domestica bork ...201728484477
1-n-histidine phosphorylation of chld by the aaa(+) chli2 stimulates magnesium chelatase activity in chlorophyll synthesis.magnesium chelatase (mg-chelatase) inserts magnesium into protoporphyrin during the biosynthesis of chlorophyll and bacteriochlorophyll. enzyme activity is reconstituted by forming two separate preactivated complexes consisting of a gun4/chlh/protoporphyrin ix substrate complex and a chli/chld enzyme 'motor' complex. formation of the chli/chld complex in both chlamydomonas reinhardtii and oryza sativa is accompanied by phosphorylation of chld by chli, but the orthologous protein complex from rho ...201728487379
rice homeodomain protein wox11 recruits a histone acetyltransferase complex to establish programs of cell proliferation of crown root meristem.shoot-borne crown roots are the major root system in cereals. previous work has shown that the wuschel-related homeobox gene wox11 is necessary and sufficient to promote rice (oryza sativa) crown root emergence and elongation. here, we show that wox11 recruits the ada2-gcn5 histone acetyltransferase module to activate downstream target genes in crown root meristem. rice ada2 and gcn5 genes are highly expressed in root meristem and are shown to be essential for cell division and growth. wox11 and ...028487409
a magnesium transporter osmgt1 plays a critical role in salt tolerance in rice.salt stress is one of the major factors limiting rice (oryza sativa) production globally. although several transporters involved in salt tolerance have been identified in rice, the mechanisms regulating their transport activity are still poorly understood. here, we show evidence that a rice mg transporter osmgt1 is required for salt tolerance probably by regulating transport activity of oshkt1;5, a key transporter for the removal of na+ from the xylem sap at the root mature zone. knockout of osm ...201728487477
novel chitinase gene loc_os11g47510 from indica rice tetep provides enhanced resistance against sheath blight pathogen rhizoctonia solani in rice.sheath blight disease (shb), caused by the fungus rhizoctonia solani kühn, is one of the most destructive diseases of rice (oryza sativa l.), causing substantial yield loss in rice. in the present study, a novel rice chitinase gene, loc_os11g47510 was cloned from qtl region of r. solani tolerant rice line tetep and used for functional validation by genetic transformation of shb susceptible japonica rice line taipei 309 (tp309). the transformants were characterized using molecular and functional ...201728487708
association mapping reveals novel genetic loci contributing to flooding tolerance during germination in indica rice.rice (oryza sativa l.) is the only cereal crop that possesses the ability to germinate under flooded or other oxygen-deficient conditions. rapid elongation of the coleoptile is a perfect response to flooding during germination, with coleoptile length differing among various rice varieties. despite multiple studies have uncovered valuable information concerning this trait by focusing on the physiological metabolism of oxygen stress, the underlying genetic mechanism still remains unknown. in the p ...201728487722
oshkt1;5 mediates na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice.salt tolerance quantitative trait loci analysis of rice has revealed that the skc1 locus, which is involved in a higher k+ /na+ ratio in shoots, corresponds to the oshkt1;5 gene encoding a na+ -selective transporter. however, physiological roles of oshkt1;5 in rice exposed to salt stress remain elusive, and no oshkt1;5 gene disruption mutants have been characterized to date. in this study, we dissected two independent t-dna insertional oshkt1;5 mutants. measurements of ion contents in tissues an ...201728488420
genome-wide sequential, evolutionary, organizational and expression analyses of phenylpropanoid biosynthesis associated myb domain transcription factors in arabidopsis.the myb gene family represents one of the largest groups of transcription factors in plants. recent evidences have also demonstrated key role of myb transcription factors in regulating the expression of major genes involved in the biosynthesis of phenylpropanoid compounds which confer biotic and abiotic stress tolerance in plant species. however, no comprehensive genome-wide analysis of the phenylpropanoid pathway-associated myb transcription factors has been reported thus far. in this study, 11 ...201728490275
osphyb-mediating novel regulatory pathway for drought tolerance in rice root identified by a global rna-seq transcriptome analysis of rice genes in response to water deficiencies.water deficiencies are one of the most serious challenges to crop productivity. to improve our understanding of soil moisture stress, we performed rna-seq analysis using roots from 4-week-old rice seedlings grown in soil that had been subjected to drought conditions for 2-3 d. in all, 1,098 genes were up-regulated in response to soil moisture stress for 3 d, which causes severe damage in root development after recovery, unlikely that of 2 d. comparison with previous transcriptome data produced i ...201728491065
development of a new heat tolerance assay system for rice spikelet sterility.reduction in rice yield caused by high temperature-induced spikelet sterility has been a serious concern in rice production. to date, several screening methods have been used, although their reproducibility is sometimes poor due to artifacts mainly caused by varietal differences in heading dates and panicle heights (i.e., the distance from the lamps).201728491121
dissection of broad-spectrum resistance of the thai rice variety jao hom nin conferred by two resistance genes against rice blast.rice (oryza sativa) is one of the most important food crops in the world. rice blast, caused by the fungal pathogen magnaporthe oryzae, is one of the most destructive rice diseases worldwide. to effectively cope with this problem, the use of rice blast resistance varieties through innovative breeding programs is the best strategy to date. the thai rice variety jao hom nin (jhn) showed broad-spectrum resistance against thai rice blast isolates. two qtls for blast resistance in jhn were reported o ...201728493203
rice black streaked dwarf virus p7-2 forms a scf complex through binding to oryza sativa skp1-like proteins, and interacts with gid2 involved in the gibberellin pathway.as a core subunit of the scf complex that promotes protein degradation through the 26s proteasome, s-phase kinase-associated protein 1 (skp1) plays important roles in multiple cellular processes in eukaryotes, including gibberellin (ga), jasmonate, ethylene, auxin and light responses. p7-2 encoded by rice black streaked dwarf virus (rbsdv), a devastating viral pathogen that causes severe symptoms in infected plants, interacts with skp1 from different plants. however, whether rbsdv p7-2 forms a s ...201728494021
rice sucrose partitioning mediated by a putative pectin methyltransferase and homogalacturonan methylesterification.homogalacturonan (hg) is the main component of pectins. hg methylesterification has recently emerged as a key determinant controlling cell attachment, organ formation, and phyllotaxy. however, whether and how hg methylesterification affects intercellular metabolite transport has rarely been reported. here, we identified and characterized knockout mutants of the rice (oryza sativa) osqua2 gene encoding a putative pectin methyltransferase. osqua2 mutants exhibit a remarkable decrease in the degree ...201728495893
genome-wide identification of histone modifiers and their expression patterns during fruit abscission in litchi.modifications to histones, including acetylation and methylation processes, play crucial roles in the regulation of gene expression in plant development as well as in stress responses. however, limited information on the enzymes catalyzing histone acetylation and methylation in non-model plants is currently available. in this study, several histone modifier (hm) types, including six histone acetyltransferases (hats), 11 histone deacetylases (hdacs), 48 histone methyltransferases (hmts), and 22 h ...201728496451
mutmapplus identified novel mutant alleles of a rice starch branching enzyme iib gene for fine-tuning of cooked rice texture.physicochemical properties of storage starch largely determine rice grain quality and food characteristics. therefore, modification of starch property is effective to fine-tune cooked rice textures. to obtain new resources with modified starch property as breeding materials, we screened a mutant population of a japonica cultivar nipponbare and found two independent mutant lines, altered gelatinization (age)1 and age2, with moderate changes in starch gelatinization property. a combination of conv ...201828499068
sequencing of bulks of segregants allows dissection of genetic control of amylose content in rice.amylose content (ac) is a key quality trait in rice. a cross between oryza glaberrima (african rice) and oryza sativa (asian rice) segregating for ac was analysed by sequencing bulks of individuals with high and low ac. snp associated with the granule bound starch synthase (gbss1) locus on chromosome 6 were polymorphic between the bulks. in particular, a g/a snp that would result in an asp to asn mutation was identified. this amino acid substitution may be responsible for differences in gbss act ...201828499072
a rice nac transcription factor promotes leaf senescence via aba biosynthesis.it is well known that abscisic acid (aba)-induced leaf senescence and premature leaf senescence negatively affect the yield of rice (oryza sativa). however, the molecular mechanism underlying this relationship, especially the upstream transcriptional network that modulates aba level during leaf senescence, remains largely unknown. here, we demonstrate a rice nac transcription factor, osnac2, that participates in aba-induced leaf senescence. overexpression of osnac2 dramatically accelerated leaf ...201728500268
dynamic cytology and transcriptional regulation of rice lamina joint development.rice (oryza sativa) leaf angle is determined by lamina joint and is an important agricultural trait determining leaf erectness and, hence, the photosynthesis efficiency and grain yield. genetic studies reveal a complex regulatory network of lamina joint development; however, the morphological changes, cytological transitions, and underlying transcriptional programming remain to be elucidated. a systemic morphological and cytological study reveals a dynamic developmental process and suggests a co ...201728500269
quantitative trait loci for large sink capacity enhance rice grain yield under free-air co2 enrichment conditions.the global atmospheric co2 concentration has been increasing annually. to determine the trait that effectively increases rice (oryza sativa l.) grain yield under increased atmospheric co2 concentrations, as predicted in the near future, we grew a chromosome segment substitution line (cssl) and a near-isogenic line (nil) producing high spikelet numbers per panicle (cssl-gn1 and nil-apo1, respectively) under free-air co2 enrichment (face) conditions and examined the effects of a large sink capacit ...201728500344
a hairy-leaf gene, blanket leaf, of wild oryza nivara increases photosynthetic water use efficiency in rice.high water use efficiency is essential to water-saving cropping. morphological traits that affect photosynthetic water use efficiency are not well known. we examined whether leaf hairiness improves photosynthetic water use efficiency in rice.201728500411
influence of sulfur on the accumulation of mercury in rice plant (oryza sativa l.) growing in mercury contaminated soils.sulfur (s) is an essential element for plant growth and its biogeochemical cycling is strongly linked to the species of heavy metals in soil. in this work, the effects of s (sulfate and elemental sulfur) treatment on the accumulation, distribution and chemical forms of hg in rice growing in hg contaminated soil were investigated. it was found that s could promote the formation of iron plaque on the root surface and decrease total mercury (t-hg) and methylmercury (mehg) accumulation in rice grain ...201728501569
effect of limestone, lignite and biochar applied alone and combined on cadmium uptake in wheat and rice under rotation in an effluent irrigated field.cadmium (cd) uptake and accumulation in crop plants, especially in wheat (triticum aestivum) and rice (oryza sativa) is one of the main concerns for food security worldwide. a field experiment was done to investigate the effects of limestone, lignite, and biochar on growth, physiology and cd uptake in wheat and rice under rotation irrigated with raw effluents. initially, each treatment was applied alone at 0.1% and combined at 0.05% each and wheat was grown in the field and then, after wheat har ...201728501770
development of a critical nitrogen dilution curve of double cropping rice in south china.the concept of critical nitrogen (nc) concentration can be implemented to diagnose in-season plant nitrogen (n) status for optimizing n fertilizer management. the nc dilution curves have been established for rice (oryza sativa l.) grown in different climatic regions, yet no attempt has been made to develop the nc dilution curve for double cropping rice regions. this study was undertaken to develop the nc dilution curves for double cropping rice in south china for assessment of in-season n status ...201728503181
isolation of rice bran lectins and characterization of their unique behavior in caco-2 cells.rice bran lectins, named as rba1 and rba2, were isolated from oryza sativa in two chromatography steps: affinity chromatography and cation-exchange chromatography. rba1 was found to be composed of a covalently linked heterodimer of 20- and 12-kda subunits, and rba2 was a noncovalently linked dimer of 12-kda subunits. both rba1 and rba2 bound to desialylated complex glycoproteins such as fetuin, α1-acid glycoprotein, and transferrin, and agalactosylated complex glycoproteins such as agalacto fetu ...201728505081
the nodulin 26-like intrinsic membrane protein osnip3;2 is involved in arsenite uptake by lateral roots in rice.previous studies have shown that the nodulin 26-like intrinsic membrane protein (nip) lsi1 (osnip2;1) is involved in arsenite [as(iii)] uptake in rice (oryza sativa). however, the role of other rice nips in as(iii) accumulation in planta remains unknown. in the present study, we investigated the role osnip3;2 in as(iii) uptake in rice. when expressed in xenopus laevis oocytes, osnip3;2 showed a high transport activity for as(iii). quantitative real-time rt-pcr showed that the expression of osnip ...201728505352
cytokinin, auxin, and abscisic acid affects sucrose metabolism conduce to de novo shoot organogenesis in rice (oryza sativa l.) callus.shoot regeneration frequency in rice callus is still low and highly diverse among rice cultivars. this study aimed to investigate the association of plant hormone signaling and sucrose uptake and metabolism in rice during callus induction and early shoot organogenesis. the immatured seeds of two rice cultivars, ai-nan-tsao 39 (ant39) and tainan 11 (tn11) are used in this study.201328510848
the extent of ds1 transposon to enrich transcriptomes and proteomes by exonization.exonization is an event which an intronic transposed element (te) provides splice sites and leads to alternatively spliced cassette exons. without disrupting of the inserted gene's function, tes can expand the proteome diversity by adding the splice variant that encodes a different, yet functional protein. previously, we found that the main contribution of ds exonization for gene divergence is not providing genetic messages but incorporating the intron sequences with different reading frame patt ...201328510860
rapid regeneration and ploidy stability of 'cv ir36' indica rice (oryza sativa. l) confers efficient protocol for in vitro callus organogenesis and agrobacterium tumefaciens mediated transformation.cereal crops are the major targets for transformation mediated crop improvement and ir36 is an early maturing, high yielding, insect and disease resistant rice variety however, it is abiotic stress sensitive. hence, development of an efficient and reproducible micropropagation system via somatic embryogenesis and agrobacterium tumefaciens mediated transformation is prerequisite to develop abiotic stress tolerant ir36. further, genetic stability of analysis of plantlets through rapd and issr and ...201328510899
genomics of interaction between the brown planthopper and rice.rice (oryza sativa l.) and the brown planthopper (nilaparvata lugens (stål)) form a model system for dissection of the mechanism of interaction between insect pest and crop. in this review, we focus on the genomics of bph-rice interaction. on the side of rice, a number of bph-resistance genes have been identified genetically. thirteen of these genes have been cloned which shed a light on the molecular basis of the interaction. on the aspect of bph, a lot of salivary proteins have been identified ...201728521948
association mapping of yield and yield-related traits under reproductive stage drought stress in rice (oryza sativa l.).the identification and introgression of major-effect qtls for grain yield under drought are some of the best and well-proven approaches for improving the drought tolerance of rice varieties. in the present study, we characterized malaysian rice germplasm for yield and yield-related traits and identified significant trait marker associations by structured association mapping.201728523639
improved stress tolerance and productivity in transgenic rice plants constitutively expressing the oryza sativa glutathione synthetase osgs under paddy field conditions.reactive oxygen species, which increase under various environmental stresses, have deleterious effects on plants. an important antioxidant, glutathione, is used to detoxify reactive oxygen species in plant cells and is mainly produced by two enzymes: gamma-glutamylcysteine synthetase (γ-ecs) and glutathione synthetase (gs). to evaluate the functional roles of the glutathione synthetase gene (osgs) in rice, we generated four independent transgenic rice plants (tg1-tg4) that overexpressed osgs und ...201728527337
influence of amendments on cd and zn uptake and accumulation in rice (oryza sativa l.) in contaminated soil.cadmium is a toxic metallic element that poses serious human health risks via consumption of contaminated agricultural products. the effect of mixtures of dicalcium phosphate and organic amendments, namely cow manure (md) and leonardite (ld), on cd and zn uptake of three rice cultivars (kdml105, kd53, and psl2) was examined in mesocosm experiments. plant growth, cd and zn accumulation, and physicochemical properties of the test soils were investigated before and after plant harvest. amendment ap ...201728528502
rna-seq analysis of gene expression for floral development in crested wheatgrass (agropyron cristatum l.).crested wheatgrass [agropyron cristatum l. (gaertn.)] is widely used for early spring grazing in western canada and the development of late maturing cultivars which maintain forage quality for a longer period is desired. however, it is difficult to manipulate the timing of floral transition, as little is known about molecular mechanism of plant maturity in this species. in this study, rna-seq and differential gene expression analysis were performed to investigate gene expression for floral initi ...201728531235
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