Publications

immunotherapy with alginate-conjugated two grass pollen extract in patients with allergic rhinoconjunctivitis.	the aim of the present study was to evaluate the clinical and immunological effects of specific immunotherapy with alginate-conjugated two grass pollen extract in patients with rhinoconjunctivitis. a total of 52 patients were admitted to the study. before and after 3 years of immunotherapy quantitative skin test (qst) to dactylis glomerata (dg) and to phleum pratense (php), variation of grass specific ige and igg4 and nasal challenge with the aqueous extract of the two grasses were compared. eac ...	2015	2382596
[conditions required for chemical binding of dactylis glomerata grass pollen to inert stable carriers].	the results of the direct rast (radio-allergo-sorbent-test) procedure used to standardise own-prepared pollen extract of the dactylis glomerata grass are presented. based on the performed standardisation, a system was set up for binding allergens to the stable inert carriers. the results of examination of the quality of d. glomerata allergen, bound covalently to paper disc, are presented also. evaluation was made in inep laboratories by comparing the d. glomerata allergen-bound discs and the cor ...	2015	1481465
[the monitoring of natural populations of dactylis glomerata l. in the area of the accident at the chernobyl atomic electric power station].	results of five years monitoring (1986-1991) of seed quality (sq) and chlorophyll mutation frequency (mf) of dactylis glomerata l., growing within 30-km zone of chernobyl npp are presented. it was shown that during initial postaccident period (1986, 1987) the criteria depend of the gamma-phone on the sampling plots. it was not found the correlation between sq, mf and duration of plant growing under condition chronic irradiation.	2016	7489103
do drying and rewetting cycles modulate effects of sulfadiazine spiked manure in soil?	naturally occurring drying-rewetting events in soil have been shown to affect the dissipation of veterinary antibiotics entering soil by manure fertilization. however, knowledge of effects on the soil microbial community structure and resistome is scarce. here, consequences of drying-rewetting cycles on effects of sulfadiazine (sdz) in soil planted with dactylis glomerata l. were investigated in microcosms. manure containing sdz or not was applied to the pregrown grass and incubated for 56 days ...	2016	27053757
aboveground insect herbivory increases plant competitive asymmetry, while belowground herbivory mitigates the effect.	insect herbivores can shift the composition of a plant community, but the mechanism underlying such shifts remains largely unexplored. a possibility is that insects alter the competitive symmetry between plant species. the effect of herbivory on competition likely depends on whether the plants are subjected to aboveground or belowground herbivory or both, and also depends on soil nitrogen levels. it is unclear how these biotic and abiotic factors interactively affect competition. in a greenhouse ...	2016	27069805
phylogenetic and diversity analysis of dactylis glomerata subspecies using ssr and it-isj markers.	the genus dactylis, an important forage crop, has a wide geographical distribution in temperate regions. while this genus is thought to include a single species, dactylis glomerata, this species encompasses many subspecies whose relationships have not been fully characterized. in this study, the genetic diversity and phylogenetic relationships of nine representative dactylis subspecies were examined using ssr and it-isj markers. in total, 21 pairs of ssr primers and 15 pairs of it-isj primers we ...	2016	27809251
comparative performance of airyscan and structured illumination superresolution microscopy in the study of the surface texture and 3d shape of pollen.	the visualization of taxonomically diagnostic features of individual pollen grains can be a challenge for many ecologically and phylogenetically important pollen types. the resolution of traditional optical microscopy is limited by the diffraction of light (250 nm), while high resolution tools such as electron microscopy are limited by laborious preparation and imaging workflows. airyscan confocal superresolution and structured illumination superresolution (sr-sim) microscopy are powerful new to ...	2016	27476493
construction of high-density genetic linkage map and identification of flowering-time qtls in orchardgrass using ssrs and slaf-seq.	orchardgrass (dactylis glomerata l.) is one of the most economically important perennial, cool-season forage species grown and pastured worldwide. high-density genetic linkage mapping is a valuable and effective method for exploring complex quantitative traits. in this study, we developed 447,177 markers based on slaf-seq and used them to perform a comparative genomics analysis. perennial ryegrass sequences were the most similar (5.02%) to orchardgrass sequences. a high-density linkage map of or ...	2016	27389619
the grass pollen season 2014 in vienna: a pilot study combining phenology, aerobiology and symptom data.	grasses (poaceae) are one of the largest plant families and are distributed worldwide. grass pollen allergy is one of the most important pollen allergies affecting large parts of the population worldwide. the grass pollen season itself is special since it is caused by the flowering of various grass species that present unique profiles of allergenicity, which assumingly plays a significant role and impact on grass pollen sensitization profiles for the allergy sufferer. the aim of this study, cond ...	2016	27317132
the effect of seaweed ecklonia maxima extract and mineral nitrogen on fodder grass chemical composition.	the objective of this study was to determine the effect of the biostimulant kelpak and different nitrogen rates on cellulose, hemicellulose and lignin contents as well as non-structural carbohydrates in orchard grass and braun's festulolium. the experiment was a split-plot arrangement with three replicates. it was set up at the experimental facility of the university of natural sciences and humanities, siedlce, in late april 2009. the following factors were examined: biostimulant with the trade ...	2016	26408121
proteome data on the microbial microbiome of grasshopper feces.	we present proteome data from the microbiota (feces) after a diet shift from a natural diverse to a monocultural meadow with dactylis glomerata. the abundant grasshopper species, chorthippus dorsatus, was taken from the wild and kept in captivity and were fed with dactylis glomerata for five days. for phytophagous insects, the efficiency of utilization of hemicellulose and cellulose depends on the gut microbiota. shifts in environmental and management conditions alter the presence and abundance ...	2016	27995170
impact of grassland management regimes on bacterial endophyte diversity differs with grass species.	most plant species are colonized by endophytic bacteria. despite their importance for plant health and growth, the response of these bacteria to grassland management regimes is still not understood. hence, we investigated the bacterial community structure in three agricultural important grass species dactylis glomerata l., festuca rubra l. and lolium perenne l. with regard to fertilizer application and different mowing frequencies. for this purpose, above-ground plant material was collected from ...	2016	26834040
genetic diversity and association of est-ssr and scot markers with rust traits in orchardgrass (dactylis glomerata l.).	orchardgrass (dactylis glomerata l.), is a well-known perennial forage species; however, rust diseases have caused a noticeable reduction in the quality and production of orchardgrass. in this study, genetic diversity was assessed and the marker-trait associations for rust were examined using 18 est-ssr and 21 scot markers in 75 orchardgrass accessions. a high level of genetic diversity was detected in orchardgrass with an average genetic diversity index of 0.369. for the est-ssr and scot marker ...	2016	26760988
comparison of rapd, issr, and aflp molecular markers to reveal and classify orchardgrass (dactylis glomerata l.) germplasm variations.	three different dna-based techniques, random amplified polymorphic dna (rapd), inter simple sequence repeat (issr) and amplified fragment length polymorphism (aflp) markers, were used for fingerprinting dactylis glomerata genotypes and for detecting genetic variation between the three different subspecies. in this study, rapd assays produced 97 bands, of which 40 were polymorphic (41.2%). the issr primers amplified 91 bands, and 54 showed polymorphism (59.3%). finally, the aflp showed 100 bands, ...	2016	27070939
productivity and carbon footprint of perennial grass-forage legume intercropping strategies with high or low nitrogen fertilizer input.	a three-season field experiment was established and repeated twice with spring barley used as cover crop for different perennial grass-legume intercrops followed by a full year pasture cropping and winter wheat after sward incorporation. two fertilization regimes were applied with plots fertilized with either a high or a low rate of mineral nitrogen (n) fertilizer. life cycle assessment (lca) was used to evaluate the carbon footprint (global warming potential) of the grassland management includi ...	2016	26479907
complementarity among four highly productive grassland species depends on resource availability.	positive species richness-productivity relationships are common in biodiversity experiments, but how resource availability modifies biodiversity effects in grass-legume mixtures composed of highly productive species is yet to be explicitly tested. we addressed this question by choosing two grasses (arrhenatherum elatius and dactylis glomerata) and two legumes (medicago × varia and onobrychis viciifolia) which are highly productive in monocultures and dominant in mixtures (the jena experiment). w ...	2016	26932467
effect of starchy or fibrous carbohydrate supplementation of orchardgrass on ruminal fermentation and methane output in continuous culture.	a 4-unit dual-flow continuous culture fermentor system was used to assess the effects of supplementing orchardgrass (dactylis glomerata l.) with 2 levels [5 and 10% of total dry matter (dm) fed] of starchy (barley grain, bar) or fibrous (beet pulp, bp) carbohydrate sources on nutrient digestibility, volatile fatty acid (vfa) production, bacterial protein synthesis, and ch4 output. treatments were randomly assigned to fermentors in a 4×4 latin square design with a 2×2 factorial arrangement using ...	2016	27060824
bacterial endophytes isolated from plants in natural oil seep soils with chronic hydrocarbon contamination.	the bacterial endophytic communities of four plants growing abundantly in soils highly contaminated by hydrocarbons were analyzed through culturable and culture-independent means. given their tolerance to the high levels of petroleum contamination at our study site, we sought evidence that achillea millefolium, solidago canadensis, trifolium aureum, and dactylis glomerata support high levels of hydrocarbon degrading endophytes. a total of 190 isolates were isolated from four plant species. the i ...	2016	27252685
mitigation of ergot vasoconstriction by clover isoflavones in goats (capra hircus).	ergot alkaloids produced by a fungal endophyte (epichloë coenophiala; formerly neotyphodium coenophialum) that infects tall fescue (lolium arundinaceum) can induce persistent constriction of the vasculature in ruminants, hindering their capability to thermo-regulate core body temperature. there is evidence that isoflavones produced by legumes can relax the vasculature, which suggests that they could relieve ergot alkaloid-induced vasoconstriction and mitigate the vulnerability to severe heat str ...	2016	26973844
fusarium dactylidis sp. nov., a novel nivalenol toxin-producing species sister to f. pseudograminearum isolated from orchard grass (dactylis glomerata) in oregon and new zealand.	the b trichothecene toxin-producing clade (b clade) of fusarium includes the etiological agents of fusarium head blight, crown rot of wheat and barley and stem and ear rot of maize. b clade isolates also have been recovered from several wild and cultivated grasses, including dactylis glomerata (orchard grass or cock's foot), one of the world's most important forage grasses. two isolates from the latter host are formally described here as f. dactylidis. phenotypically f. dactylidis most closely r ...	2016	25550300
combining drought survival via summer dormancy and annual biomass productivity in dactylis glomerata l.	under mediterranean climates, the best strategy to produce rain-fed fodder crops is to develop perennial drought resistant varieties. summer dormancy present in native germplasm has been shown to confer a high level of survival under severe drought. nevertheless it has also been shown to be negatively correlated with annual biomass productivity. the aim of this study was to analyze the correlations between summer dormancy and annual biomass productivity related traits and to identify quantitativ ...	2016	26904054
use of two grasses for the phytoremediation of aqueous solutions polluted with terbuthylazine.	the capacity of two grasses, tall fescue (festuca arundinacea) and orchardgrass (dactylis glomerata), to remove terbuthylazine (tba) from polluted solutions has been assessed in hydroponic cultures. different tba concentrations (0.06, 0.31, 0.62, and 1.24 mg/l) were chosen to test the capacity of the two grasses to resist the chemical. aerial biomass, effective concentrations (to cause reductions of 10, 50, and 90% of plant aerial biomass) and chlorophylls contents of orchardgrass were found to ...	2016	26934386
[the occurrence of the cocksfoot streak virus].		2016	4912699
iranian johnsongrass mosaic virus: the complete genome sequence, molecular and biological characterization, and comparison of coat protein gene sequences.	iranian johnsongrass mosaic virus (ijmv) is one of the most prevalent viruses causing maize mosaic disease in iran. an ijmv isolate, maz-bah, was obtained from the maize showing mosaic symptoms in mazandaran, north of iran. the complete genomic sequence of maz-bah is 9544 nucleotides, excluding the poly(a) tail. it contains one single open reading frame of 9165 nucleotides and encodes a large polyprotein of 3054 amino acids, flanked by a 5'-untranslated region (utr) of 143 nucleotides and a 3'-u ...	2017	27632283
p1-independent replication and local movement of rice yellow mottle virus in host and non-host plant species.	sobemovirus p1 protein, characterized previously as a suppressor of posttranscriptional gene silencing, is required for systemic virus spread and infection in plants. mutations in the orf1 initiation codon do not affect viral replication indicating p1 is not necessary for this process. wild type, recombinant and p1 deletion mutants of cocksfoot mottle virus and rice yellow mottle virus were used to infect oat, rice, wheat, barley, arabidopsis thaliana and nicotiana benthamiana plants. wild type ...	2017	27960111
using plant wax markers to estimate the diet composition of grazing holstein dairy cows.	the objective of this study was to test whether diet selection of dairy cows under grazing conditions could be estimated using plant wax markers. furthermore, differences between 2 cow strains and the effect of concentrate supplementation on plant species selection were investigated. the experiment was a study with a crossover design performed on an organic farm with 12 swiss holstein cows and 12 new zealand holstein cows. both experimental periods consisted of a 21-d adaptation and a 7-d measur ...	2017	27939548
allergy to grass pollen: mapping of dactylis glomerata and phleum pratense allergens for dogs by two-dimensional immunoblotting.	much less is known about grass-pollen allergens to dogs, when compared with humans. genetic-based patterns might play an important role in sensitization profiles, conditioning the success of allergen-specific immunotherapy.	2017	28261033
genome-wide association study of rust traits in orchardgrass using slaf-seq technology.	while orchardgrass (dactylis glomerata l.) is a well-known perennial forage species, rust diseases cause serious reductions in the yield and quality of orchardgrass; however, genetic mechanisms of rust resistance are not well understood in orchardgrass.	2017	28250720
tracing the fate and transport of secondary plant metabolites in a laboratory mesocosm experiment by employing mass spectrometric imaging.	mass spectrometric imaging (msi) has received considerable attention in recent years, since it allows the molecular mapping of various compound classes, such as proteins, peptides, glycans, secondary metabolites, lipids, and drugs in animal, human, or plant tissue sections. in the present study, the application of laser-based msi analysis of secondary plant metabolites to monitor their transport from the grass leaves of dactylis glomerata, over the crop of the grasshopper chorthippus dorsatus to ...	2017	28357483
dynamics of runoff and sediment trapping performance of vegetative filter strips: run-on experiments and modeling.	vegetative filter strips (vfss) are a labor-saving and cost-effective agricultural best management practice to trap water runoff and sediment from the source areas. they also provide forage and/or fuel and are therefore potentially profitable for land owners. vfss are however a dynamic system: the runoff delivery ratio (rdr) and sediment delivery ratio (sdr) vary with growth stage and vegetation types. the impacts of vegetation characteristics as well as soil physical properties modified by vege ...	2017	28342418
the effects of plant nutritional strategy on soil microbial denitrification activity through rhizosphere primary metabolites.	the aim of this study was to determine (i) whether plant nutritional strategy affects the composition of primary metabolites exuded into the rhizosphere and (ii) their impact on denitrification activity in soil. we answer this question by analysing primary metabolite contents extracted from the root-adhering soil (ras) and the roots of three grasses representing different nutrient management strategies: conservative (festuca paniculata), intermediate (bromus erectus) and exploitative (dactylis g ...	2017	28334144
effect of mixed hay supplementation during fattening on carcass traits and meat quality of hanwoo steers.	this study was aim to observe the effects of feeding mixed local hay (mh) consisted of 55% orchard grass (dactylis glomerata l.), 35% tall fescue (festuca arundinacea) and 10% red clover (trifolium pratense) to hanwoo steers on performance, carcass characteristics and meat quality (longissimus thoracis) compared with feeding imported timothy hay (th) and local rice straw (rs).	2017	28293430
water use efficiency and shoot biomass production under water limitation is negatively correlated to the discrimination against (13)c in the c3 grasses dactylis glomerata, festuca arundinacea and phalaris arundinacea.	climate change impacts rainfall patterns which may lead to drought stress in rain-fed agricultural systems. crops with higher drought tolerance are required on marginal land with low precipitation or on soils with low water retention used for biomass production. it is essential to obtain plant breeding tools, which can identify genotypes with improved drought tolerance and water use efficiency (wue). in c3 plant species, the variation in discrimination against (13)c (δ(13)c) during photosynthesi ...	2017	28152389
bacterial endophyte communities of three agricultural important grass species differ in their response towards management regimes.	endophytic bacteria are critical for plant growth and health. however, compositional and functional responses of bacterial endophyte communities towards agricultural practices are still poorly understood. hence, we analyzed the influence of fertilizer application and mowing frequency on bacterial endophytes in three agriculturally important grass species. for this purpose, we examined bacterial endophytic communities in aerial plant parts of dactylis glomerata l., festuca rubra l., and lolium pe ...	2017	28102323
aflp assessment of genetic variability and relationships in an asian wild germplasm collection of dactylis glomerata l.	orchardgrass (dactylis glomerata l.), an excellent perennial and cool season forage species distributed in most temperate regions, has been cultivated widely in western china. amplified fragment length polymorphism markers were employed to determine the genetic variability and population structure among 41 indigenous orchardgrass accessions from central asia and western china. on the basis of 531 polymorphic fragments resulted from eight primer combinations, polymorphic information content (pic) ...	2017	28215522
effect of feeding warm-season annuals with orchardgrass on ruminal fermentation and methane output in continuous culture.	a 4-unit, dual-flow continuous culture fermentor system was used to assess nutrient digestibility, volatile fatty acids (vfa) production, bacterial protein synthesis, and methane (ch4) output of warm-season annual grasses. treatments were randomly assigned to fermentors in a 4 × 4 latin square design using 7 d for adaptation to treatment and 3 d for sample collection. treatments were (1) 100% orchardgrass (dactylis glomerata l.; ord); (2) 50% orchardgrass + 50% japanese millet [echinochloa escul ...	2017	27939544
responses of plasma glucose metabolism to exogenous insulin infusion in sheep-fed forage herb plantain and exposed to heat.	the use of herbal plants as traditional medicines has a century long history. plantain (plantago lanceolata l.) is a perennial herb containing bioactive components with free radical scavenging activities. an isotope dilution technique using [u-13c]glucose was conducted to determine the effect of plantain on the responses of plasma glucose metabolism to exogenous insulin infusion in sheep. six crossbred sheep (three wethers and three ewes; mean initial bw=40±2 kg) were fed either a mixed hay of o ...	2017	28091361