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protein-rna linkage and post-translational modifications of two sobemovirus vpgs.sobemoviruses possess a viral genome-linked protein (vpg) attached to the 5' end of viral rna. vpg is processed from the viral polyprotein. in the current study, cocksfoot mottle virus (cfmv) and rice yellow mottle virus (rymv) vpgs were purified from virions and analysed by mass spectrometry. the cleavage sites in the polyprotein and thereof the termini of vpg were experimentally proven. the lengths of the mature vpgs were determined to be 78 and 79 aa residues, respectively. the amino acid res ...201021068217
optimization of culture conditions of arnica montana l.: effects of mycorrhizal fungi and competing plants.arnica montana is a rare plant that needs special protection because of its intensive harvesting for medicinal purposes. the present work was aimed at finding optimal culture conditions for arnica plants in order to enable their successful reintroduction into their natural stands. plants were cultivated under controlled greenhouse conditions on substrata with different nitrogen (n) concentration. as arnica is always colonized by arbuscular mycorrhizal fungi (amf) in nature, a fact that has been ...201019838743
enzymatic activity of a mine soil varies according to vegetation cover and level of compost applied.we applied three doses of compost from mixed municipal solid waste (0, 15, and 30 g kg(-1) of soil) to a soil developed on pyrite mine wastes. part of the soil was planted with young erica australis l. collected at the mine; part was fertilized with n-p-k-mg and sown with dactylis glomerata l .bare soil without mineral fertilization was included in the experiment, as well. compost application to bare soil increased ph, provided plant nutrients, and enhanced the activity of the six soil enzymes t ...201020734914
plant resource-use strategies: the importance of phenotypic plasticity in response to a productivity gradient for two subalpine species.functional traits are indicators of plant interactions with their environment and the resource-use strategies of species can be defined through some key functional traits. the importance of genetic variability and phenotypic plasticity in trait variations in response to a common environmental change was investigated in two subalpine species.201020682576
uptake and transformation of soil [14c]-trinitrotoluene by cool-season grasses.this study investigated the fate and uptake of [(14)c]-tnt from soil into orchardgrass (dactylis glomerata), perennial ryegrass (lolium perenne), and tall fescue (festuca arundinacea) over a one year period in a greenhouse-controlled environment. pots (n = 4 for each grass, containing 10 mg cold tnt/kg soil + 1.2 mg [(14)c]-tnt/kg soil and controls with no tnt) were exposed to light and temperature conditions typical of june at 45 degrees n for 369 days. three plant harvests were made (63, 181, ...201020666491
effects of spontaneous heating on estimates of total digestible nutrients for alfalfa-orchardgrass hays packaged in large round bales.large round or large square hay packages are more likely to heat spontaneously during storage than hay packaged in conventional (45 kg) bales, and the effects of this phenomenon on the associated energy estimates for these hays can be severe. our objectives for this project were to assess the relationship between estimates of total digestible nutrients (tdn) and spontaneous heating and to describe any important differences in energy estimates that may result specifically from 2 methods of estima ...201020630254
detection of cereal yellow dwarf virus using small interfering rnas and enhanced infection rate with cocksfoot streak virus in wild cocksfoot grass (dactylis glomerata).small rna sequences were obtained from leaf extracts of wild dactylis glomerata (cocksfoot grass) using deep sequencing (454 life sciences, roche diagnostics), and were screened against virus sequences in genbank using a local blastn search program (bioedit). putative small interfering (si)rnas complementary in sequence to cereal yellow dwarf virus (cydv, genus luteovirus) genomes were identified. primer sequences were made against the "high scoring" sirna sequences and rt-pcr was used to amplif ...201020558208
effects of spontaneous heating on forage protein fractions and in situ disappearance kinetics of crude protein for alfalfa-orchardgrass hays packaged in large round bales.during 2006 and 2007, forages from 3 individual hay harvests were used to assess the effects of spontaneous heating on concentrations of crude protein (cp), neutral detergent insoluble cp (ndicp), acid detergent insoluble cp (adicp), and in situ disappearance kinetics of cp and ndicp for large round bales of mixed alfalfa (medicago sativa l.) and orchardgrass (dactylis glomerata l.). over the 3 harvests, 96 large round bales were made at preset bale diameters of 0.9, 1.2, or 1.5m and at moisture ...201020172236
occurrence of associative effects between grasses and legumes in binary mixtures on in vitro rumen fermentation characteristics.when animals are fed a grass and legume mixture, digestive interactions can occur in the rumen between the substrates contained in the different plants, and the response of the animal to the combination of forages can differ from the balanced median values of their components considered individually. our objective was to assess the associative effects between temperate forages in 8 grass-legume binary combinations on in vitro rumen fermentation characteristics to highlight synergies or antagonis ...201121415423
identification and characterization of lactic acid bacteria isolated from mixed pasture of timothy and orchardgrass, and its badly preserved silage.in order to understand the relationship between lactic acid bacteria (lab) species and silage fermentation, a total of 65 lab strains isolated from mixed pasture of timothy (phleum pratense l.) and orchardgrass (dactylis glomerata l.), and its badly preserved silages were subjected to phenotypic and genetic analysis. according to these analyses, the isolates were divided into 13 groups, including enterococcus gallinarum, lactobacillus acidipiscis, l. coryniformis subsp. coryniformis, l. corynifo ...201122515692
within season and carry-over effects following exposure of grassland species mixtures to increasing background ozone.few studies have investigated effects of increased background ozone in the absence of episodic peaks, despite a predicted increase throughout the northern hemisphere over the coming decades. in this study leontodon hispidus was grown with anthoxanthum odoratum or dactylis glomerata and exposed in the uk to one of eight background ozone concentrations for 20 weeks in solardomes. seasonal mean ozone concentrations ranged from 21.4 to 102.5 ppb. ozone-induced senescence of l. hispidus was enhanced ...201121763041
orchardgrass (dactylis glomerata l.) est and ssr marker development, annotation, and transferability.orchardgrass, or cocksfoot [dactylis glomerata (l.)], has been naturalized on nearly every continent and is a commonly used species for forage and hay production. all major cultivated varieties of orchardgrass are autotetraploid, and few tools or information are available for functional and comparative genetic analyses and improvement of the species. to improve the genetic resources for orchardgrass, we have developed an est library and ssr markers from salt, drought, and cold stressed tissues. ...201121465186
genetic maps of ssr and srap markers in diploid orchardgrass (dactylis glomerata l.) using the pseudo-testcross strategy.orchardgrass (dactylis glomerata l.) is one of the most important cool-season forage grasses commonly grown throughout the temperate regions of the world. the objective of this work was to construct a diploid (2n = 2x = 14) orchardgrass genetic linkage map useful as a framework for basic genetic studies and plant breeding. a combination of simple sequence repeat (ssr) and sequence-related amplified polymorphism (srap) molecular markers were used for map construction. the linkage relationships am ...201121423284
transformation of epichloë typhina by electroporation of conidia.choke, caused by the endophytic fungus epichloë typhina, is an important disease affecting orchardgrass (dactylis glomerata l.) seed production in the willamette valley. little is known concerning the conditions necessary for successful infection of orchardgrass by e. typhina. detection of e. typhina in plants early in the disease cycle can be difficult due to the sparse distribution of hyphae in the plant. therefore, a sensitive method to detect fungal infection in plants would provide an inval ...201121375770
two new species of rhynchosporium.rhynchosporium consists of two species, r. secalis and r. orthosporum. both are pathogens of grasses with r. secalis infecting a variety of poaceae hosts and r. orthosporum infecting dactylis glomerata. phylogenetic analyses of multilocus dna sequence data on r. secalis isolates originating from cultivated barley, rye, triticale and other grasses, including agropyron spp., bromus diandrus and hordeum spp., resolved the monophyletic groups into three species according to their respective hosts. h ...201120943529
the distribution of hla alleles among children with atopic asthma in croatia.allergic asthma is a multifactorial disease involving well known environmental factors and less identified genetic components. in several studies the hla genes have been implicated in the development of asthma and atopy, but the importance of these associations remains unclear. the aim of the present study was to analyse the distribution of specificities at hla class i loci (-a and -b) and hla class ii locus (-drb1) in a group of 143 croatian children with atopic asthma, regarding total serum ig ...201122397267
skin prick testing to aeroallergen extracts: what is the optimal panel in children and adolescents in turkey?background/objective: the skin prick test (spt) is the standard tool for the diagnosis of ige-mediated allergic diseases. the number and spectrum of allergen extracts to be applied for the diagnosis of sensitized patients in order to achieve the most cost-effective and informative results are critical from both economic and scientific viewpoints. methods: in order to determine the minimum test battery panel necessary to cover at least 95% of the cases of spt sensitization in children and adolesc ...201122123205
pollen proteases compromise the airway epithelial barrier through degradation of transmembrane adhesion proteins and lung bioactive peptides.to cite this article: vinhas r, cortes l, cardoso i, mendes vm, manadas b, todo-bom a, pires e, veríssimo p. pollen proteases compromise the airway epithelial barrier through degradation of transmembrane adhesion proteins and lung bioactive peptides. allergy 2011; doi: 10.1111/j.1398-9995.2011.02598.x. abstract: background:  allergic disorders, such as seasonal rhinitis and asthma, are increasing causes of morbidity worldwide and often result from exposure to airborne pollen. pollen allergy has ...201121480927
effect of ensiled hop (humulus lupulus l.) residues on plasma acetate turnover rate in sheep.an isotope dilution method using [1-(13) c]sodium acetate was applied to determine the effect of feeding ensiled hop (humulus lupulus l.) residues on plasma acetate turnover rate in six adult crossbred sheep. the sheep were fed 63 g/kg body weight (bw)(0.75) /day of either mixed hay of orchardgrass (dactylis glomerata l.) and reed canarygrass (phalaris arundinacea l.) and round bale silage at 3:1 ratio (hay-diet), or another where round bale silage was replaced by ensiled hop residues (hop-diet) ...201121615839
enhanced nitrogen deposition exacerbates the negative effect of increasing background ozone in dactylis glomerata, but not ranunculus acris.the combined impacts of simulated increased nitrogen (n) deposition (75kgnha(-1)yr(-1)) and increasing background ozone (o(3)) were studied using two mesotrophic grassland species (dactylis glomerata and ranunculus acris) in solardomes, by means of eight o(3) treatments ranging from 15.5ppb to 92.7ppb (24h average mean). a-c(i) curves were constructed for each species to gauge effects on photosynthetic efficiency and capacity, and effects on biomass partitioning were determined after 14 weeks. i ...201121741736
ammonia volatilization from surface-banded and broadcast application of liquid dairy manure on grass forage.manure can provide valuable nutrients, especially n, for grass forage, but high nh, volatilization losses from standard surface-broadcast application limits n availability and raises environmental concerns. eight field trials were conducted to evaluate the emission of nh, from liquid dairy manure, either surface broadcast or applied in narrow surface bands with a trailing-foot implement. manure was applied using both techniques at rates of approximately 25 and 50 m3 ha(-1) on either orchardgrass ...201121520744
do sheep (ovis aries) categorize plant species according to botanical family?the ability of grazing herbivores to assign food types to categories by relying on certain relevant criteria could considerably reduce cognitive demand and increase their foraging efficiency when selecting among many different plant items. grasses and legumes differ functionally in vegetation communities as well as in nutritive value. we aimed to determine whether sheep can generalize an aversion they learnt for a grass or a legume species to another species of the same functional type and conse ...201121203791
Glycoproteins are species-specific markers and major IgE reactants in grass pollens.Grass pollen allergic patients are concomitantly exposed and sensitized to pollens from multiple Pooideae (i.e. common grass) species. As such, they are currently desensitized by allergen-specific immunotherapy using extracts made from mixes of pollens from Anthoxanthum odoratum, Dactylis glomerata, Lolium perenne, Phleum pratense and Poa pratensis. Herein, we demonstrate that species-specific glycoprotein patterns are documented by 1D and 2D electrophoresis and Western blotting analysis, which ...201121951299
traceability of grass feeding in beef: terpenes, 2,3-octanedione and skatole accumulation in adipose tissue of young bulls.the development of analytical methods to verify the production system of meat products requires the identification of biomarkers that can trace the product's origin, and secondly the factors that govern the deposition of these markers in animal tissue need to be defined. in this study, 2,3-octanedione, skatole and terpenes were selected as biomarkers, and their deposition was investigated in bull calves reared under three different strategies. all of the animals were reared indoors until approxi ...201122439961
allelochemical stress inhibits growth, leaf water relations, psii photochemistry, non-photochemical fluorescence quenching, and heat energy dissipation in three c3 perennial species.in this study, the effect of two allelochemicals, benzoxazolin-2(3h)-one (boa) and cinnamic acid (ca), on different physiological and morphological characteristics of 1-month-old c(3) plant species (dactylis glomerata, lolium perenne, and rumex acetosa) was analysed. boa inhibited the shoot length of d. glomerata, l. perenne, and r. acetosa by 49%, 19%, and 19% of the control. the root length of d. glomerata, l. perenne, and r. acetosa growing in the presence of 1.5 mm boa and ca was decreased c ...201121659663
cocksfoot mottle sobemovirus establishes infection through the phloem.cocksfoot mottle virus (cfmv) localization in oat plants was analyzed during three weeks post infection by immunohistochemical staining to follow its spread through different tissues. in early stages of infection, the virus was first detectable in phloem parenchyma and bundle sheath cells of inoculated leaves. bundle sheath and phloem parenchyma were also the cell types where the virus was first detected in stems and systemic leaves of infected plants. in later stages of infection, cfmv spread a ...201222425583
leaching and ponding of viral contaminants following land application of biosolids on sandy-loam soil.much of the land available for application of biosolids is cropland near urban areas. biosolids are often applied on hay or grassland during the growing season or on corn ground before planting or after harvest in the fall. in this study, mesophilic anaerobic digested (mad) biosolids were applied at 56,000 l/ha on a sandy-loam soil over large containment lysimeters seeded to perennial covers of orchardgrass (dactylis glomerata l.), switchgrass (panicum virgatum), or planted annually to maize (ze ...201222885066
pretreatment as the crucial step for a cellulosic ethanol biorefinery: testing the efficiency of wet explosion on different types of biomass.the efficiency of wet explosion applied as modified dilute acid pretreatment at previously identified reference conditions (150°c, 0.3% h(2)so(4), 15 min) was investigated on lucerne, ryegrass, fescue grass, cocksfoot grass, rye fescue, forage grass, and wheat straw in order to identify their potential as feedstock for cellulosic bioethanol production. after pretreatment, cellulose recovery was more than 95% for all biomass while enzymatic convertibility of cellulose ranged from 40% to 80%. lowe ...201222989639
zymoseptoria ardabiliae and z. pseudotritici, two progenitor species of the septoria tritici leaf blotch fungus z. tritici (synonym: mycosphaerella graminicola).zymoseptoria is a newly described genus that includes the prominent wheat pathogen zymoseptoria tritici (synonyms mycosphaerella graminicola and septoria tritici). studies indicated that the center of origin of z. tritici is in the middle east where this important pathogen emerged during the domestication of wheat. several zymoseptoria species have been found on uncultivated grasses in the middle east, and in this article we describe two new zymoseptoria species from iran. these species, isolate ...201222675045
expansion of a globally pervasive grass occurs without substantial trait differences between home and away populations.the global expansion of species beyond their ancestral ranges can derive from mechanisms that are trait-based (e.g., post-establishment evolved differences compared to home populations) or circumstantial (e.g., propagule pressure, with no trait-based differences). these mechanisms can be difficult to distinguish following establishment, but each makes unique predictions regarding trait similarity between ancestral ('home') and introduced ('away') populations. here, we tested for trait-based popu ...201222669263
a genetic linkage map of tetraploid orchardgrass (dactylis glomerata l.) and quantitative trait loci for heading date.orchardgrass (dactylis glomerata l.), or cocksfoot, is indigenous to eurasia and northern africa, but has been naturalized on nearly every continent and is one of the top perennial forage grasses grown worldwide. to improve the understanding of genetic architecture of orchardgrass and provide a template for heading date candidate gene search in this species, the goals of the present study were to construct a tetraploid orchardgrass genetic linkage map and identify quantitative trait loci associa ...201222551303
genetic variation and comparison of orchardgrass (dactylis glomerata l.) cultivars and wild accessions as revealed by ssr markers.orchardgrass is a highly variable, perennial forage grass that is cultivated throughout temperate and subtropical regions of the world. despite its economic importance, the genetic relationship and distance among and within cultivars are largely unknown but would be of great interest for breeding programs. we investigated the molecular variation and structure of cultivar populations, compared the level of genetic diversity among cultivars (baoxing, anba, bote, and kaimo), subspecies (dactylis gl ...201222427034
reduced soil water availability did not protect two competing grassland species from the negative effects of increasing background ozone.two common (semi-) natural temperate grassland species, dactylis glomerata and ranunculus acris, were grown in competition and exposed to two watering regimes: well-watered (ww, 20-40% v/v) and reduced-watered (rw, 7.5-20% v/v) in combination with eight ozone treatments ranging from pre-industrial to predicted 2100 background levels. for both species there was a significant increase in leaf damage with increasing background ozone concentration. rw had no protective effect against increasing leve ...201222420992
redox state of low-molecular-weight thiols and disulphides during somatic embryogenesis of salt-treated suspension cultures of dactylis glomerata l.the tripeptide antioxidant γ-l-glutamyl-l-cysteinyl-glycine, or glutathione (gsh), serves a central role in ros scavenging and oxidative signalling. here, gsh, glutathione disulphide (gssg), and other low-molecular-weight (lmw) thiols and their corresponding disulphides were studied in embryogenic suspension cultures of dactylis glomerata l. subjected to moderate (0.085 m nacl) or severe (0.17 m nacl) salt stress. total glutathione (gsh + gssg) concentrations and redox state were associated with ...201222348546
comparison of a colorimetric and a high-performance liquid chromatography method for the determination of fructan in pasture grasses for horses.pasture (fresh or conserved as hay/haylage) forms the basis of most equid diets and contains varying amounts (0 to ≥ 200 g kg⁻¹ dry matter (dm) or more) of fructans. over-consumption of fructan is associated with the onset of laminitis in equids, an agonizing condition that may necessitate euthanasia. to enable appropriate dietary management of animals susceptible to laminitis, it is essential that fructans can be properly quantified in fresh and conserved pasture. for research purposes, fructan ...201222297902
dynamic trajectories of growth and nitrogen capture by competing plants.although dynamic, plant competition is usually estimated as biomass differences at a single, arbitrary time; resource capture is rarely measured. this restricted approach perpetuates uncertainty. to address this problem, we characterized the competitive dynamics of dactylis glomerata and plantago lanceolata as continuous trajectories of biomass production and nitrogen (n) capture. plants were grown together or in isolation. biomass and n content were measured at 17 harvests up to 76 d after sowi ...201222236094
using natural gradients to infer a potential response to climate change: an example on the reproductive performance of dactylis glomerata l.an understanding of the climate conditions governing spatial variation in the reproductive performance of plants can provide important information about the factors characterizing plant community structure, especially in the context of climate change. this study focuses on the effect of climate on the sexual reproductive output of dactylis glomerata l., a perennial grass species widely distributed throughout temperate regions. an indirect space-for-time substitution procedure was used. sixty mou ...201224832520
effect of incremental flaxseed supplementation of an herbage diet on methane output and ruminal fermentation in continuous culture.a 4-unit dual-flow continuous culture fermentor system was used to assess the effect of increasing flaxseed (linum usitatissimum) supplementation of an herbage-based diet on nutrient digestibility, microbial n synthesis, and methane (ch(4)) output. treatments were randomly assigned to fermentors in a 4 × 4 latin square design, with 7d for diet adaptation and 3d for data and sample collection. treatments were 0, 5, 10, and 15% ground flaxseed supplementation of an orchardgrass (dactylis glomerata ...201222720950
synthesis and herbicidal activity of substituted pyrazole isothiocyanates.isothiocyanates and substituted pyrazoles were combined to form a series of novel isothiocyanates with highly effective herbicidal activity. the target compounds were analyzed by elemental analysis, 1h-nmr, ei-ms and ir spectroscopy. the synthesized compounds, particularly compounds 3-1 and 3-7, exhibited good herbicidal activities against four weeds. the ec(50) values of compound 3-1 against echinochloa crusgalli l., cyperus iria l., dactylis glomerata l., and trifolium repens l. were 64.32, 65 ...201223075815
antimony, arsenic and lead distribution in soils and plants of an agricultural area impacted by former mining activities.an agricultural area impacted by the former exploitation of an arsenical lead-antimony deposit was studied in order to assess the current and eventual environmental and health impacts. samples of soils and cultivated (wheat) and spontaneously growing plants were collected at different distances from the mine pits and analyzed for the toxic element content and distribution. the soil total concentrations of sb, as and pb found in the uppermost soil layer (14.1-324, 246-758 and 757-10,660 mg kg(-1) ...201223063636
herbaceous vegetation productivity, persistence, and metals uptake on a biosolids-amended mine soil.the selection of plant species is critical for the successful establishment and long-term maintenance of vegetation on reclaimed surface mined soils. a study was conducted to assess the capability of 16 forage grass and legume species in monocultures and mixes to establish and thrive on a reclaimed appalachian surface mine amended with biosolids. the 0.15-ha coarse-textured, rocky, non-acid forming mined site was prepared for planting by grading to a 2% slope and amending sandstone overburden ma ...201216151233
role of rainfall intensity and hydrology in nutrient transport via surface runoff.loss of soil nutrients in runoff accelerates eutrophication of surface waters. this study evaluated p and n in surface runoff in relation to rainfall intensity and hydrology for two soils along a single hillslope. experiments were initiated on 1- by 2-m plots at foot-slope (6%) and mid-slope (30%) positions within an alfalfa (medicago sativa l.)-orchardgrass (dactylis glomerata l.) field. rain simulations (2.9 and 7.0 cm h(-1)) were conducted under wet (spring) and dry (late-summer) conditions. ...201316825444
an immunoblotting analysis of cross-reactivity between melon, and plantago and grass pollens.it is known that most patients with type i allergy to pollens also suffer intolerance to fruits. recently, an epidemiological and cap-inhibition study has shown a new clustering of allergy between melon and plantago and grass pollens. the aim of the present study was to confirm these results by immunoblotting analysis and inhibition of immunoblotting. sera from 3 patients with confirmed allergy to melon, and dactylis glomerata and plantago lanceolata pollens were used for the in vitro studies. s ...20139015782
effect of manure application timing, crop, and soil type on phosphorus leaching.phosphorus (p) leaching losses from manure applications may be of concern when artificial drainage systems allow for hydrologic short-cuts to surface waters. this study quantified p leaching losses from liquid manure applications on two soil textural extremes, a clay loam and loamy sand soil, as affected by cropping system and timing of application. for each soil type, manure was applied at an annual rate of 93 800 l ha(-1) on replicated drained plots under maize (zea mays l.) in early fall, lat ...201315224946
nitrogen- vs. phosphorus-based dairy manure applications to field crops: nitrate and phosphorus leaching and soil phosphorus accumulation.management of animal manures to provide nutrients for crop growth has generally been based on crop n needs. however, because manures have a lower n/p ratio than most harvested crops, n-based manure management often oversupplies the crop-soil system with p, which can be lost into the environment and contribute to eutrophication of water bodies. we examined the effects of n- vs. p-based manure applications on n and p uptake by alfalfa (medicago sativa l.), corn (zea mays l.) for silage, and orchar ...201317071901
[skin reactivity in the histamine-equivalent skin test in hypersensitivity to grass pollen].skin prick-test has been accepted as one of diagnostic criteria for atopic diseases. in accordance with the recommendations of the eaaci (european academy of allergy and clinical immunology), the subcommittee for skin testing, allergen specific skin prick-test should be estimated in relation to positive control (histamine hydrochloride in the hep system/histamine equivalent prick/) [1-4]. the purpose of this study was to establish a recording system of skin sensitivity to allergen by using hep m ...201311089421
soil-soiln simulations of water drainage and nitrate nitrogen transport from soil core lysimeters.water resources protection from nitrate nitrogen (no3-n) contamination is an important public concern and a major national environmental issue. the abilities of the soil-soiln model to simulate water drainage and nitrate n fluxes from orchardgrass (dactylis glomerata l.) were evaluated using data from a 3-yr field experiment. the soil is classified as a hagerstown silt loam soil (fine, mixed, semiactive, mesic typic hapludalf). nitrate losses below the 1-m depth from n-fertilized grazed orchardg ...201311285920
[obtaining mutations with cytoplasmic male sterility (cms) in dactylis glomerata l. under the influence of chemical mutagens].when treating seeds of orchard grass with definite concentrations of nitrosoethylurea (neu), dimethyl sulphate (dms) and ethylene imine (ei) in m1 and m2 the plants with cms as well as sterility fixators to them are obtained. proportionality of mutagenic action and stimulation effect of these substances are found. the greatest amount of sterility fixators is found in m1 among plants the seeds of which were treated with neu and ei; in m2 the fixators are found among plants obtained after treating ...20131030857
effect of hay steaming on forage nutritive values and dry matter intake by horses.management strategies for horses with respiratory disease include soaking hay before feeding. hay steaming is an alternative to this practice; however, little is known about its impact on forage nutritive values or intake. the objective was to determine the effect of steaming on forage nutritive value and intake by horses. two alfalfa (medicago sativa l.)-orchardgrass (dactylis glomerata l.) mixed hays were evaluated: a low moldy (nm) and moderately moldy (mm) hay. six mature horses were used in ...201324146155
identification of orchardgrass (dactylis glomerata l.) cultivars by using simple sequence repeat markers.the accurate identification of orchardgrass (dactylis glomerata l.) cultivars is necessary to ensure purity for consumers, the effective utilization of cultivars, and to protect the intellectual property for breeders. therefore, this study aimed to use ssr to construct dna fingerprinting of orchardgrass cultivars. the genetic diversity of 32 orchardgrass cultivars originated from 21 countries, but grown in china, was assessed using a set of 29 ssr markers distributed across 9 linkage groups of t ...201324301771
genetic diversity of natural orchardgrass (dactylis glomerata l.) populations in three regions in europe.dactylis glomerata (orchardgrass or cocksfoot) is a forage crop of agronomic importance comprising high phenotypic plasticity and variability. although the genus dactylis has been studied quite well within the past century, little is known about the genetic diversity and population patterns of natural populations from geographically distinct grassland regions in europe. the objectives of this study were to test the ploidy level of 59 natural and semi-natural populations of d. glomerata, to inves ...201324165514
evolutionary relationships between rhynchosporium lolii sp. nov. and other rhynchosporium species on grasses.the fungal genus rhynchosporium (causative agent of leaf blotch) contains several host-specialised species, including r. commune (colonising barley and brome-grass), r. agropyri (couch-grass), r. secalis (rye and triticale) and the more distantly related r. orthosporum (cocksfoot). this study used molecular fingerprinting, multilocus dna sequence data, conidial morphology, host range tests and scanning electron microscopy to investigate the relationship between rhynchosporium species on ryegrass ...201324146740
lactobacillus silagei sp. nov., isolated from orchardgrass silage.a gram-reaction-positive, facultatively anaerobic, non-spore-forming and catalase-negative rod-shaped bacterial strain, designated iwt126(t), was isolated from orchardgrass (dactylis glomerata l.) silage preserved in hachimantai, iwate, japan. the isolate showed growth at 15-45 °c, ph 3.5-7.5 and with 4.0 % (w/v) nacl. the cell wall peptidoglycan did not contain meso-diaminopimelic acid, and the dna g+c content was 45.6 mol%. the major cellular fatty acids were c16 : 0 and c19 : 1 cyclo 9,10. ba ...201323919960
infectious transcripts of an asymptomatic panicovirus identified from a metagenomic survey.a new panicovirus was identified from a metagenomic survey of plant viruses in the tallgrass prairie preserve (tpp), osage county, oklahoma, usa. thin paspalum asymptomatic virus (tpav) was isolated from paspalum setaceum var. muehlenbergii. the complete 4195 nucleotide sequence encodes six proteins of 44, 109, 8, 6.8, 26, and 15kda, whose genes are similar in size and location to those of panicum mosaic virus (pmv) and cocksfoot mild mosaic virus (cmmv). infectious transcripts made from cdnas o ...201323774065
tailoring wet explosion process parameters for the pretreatment of cocksfoot grass for high sugar yields.the pretreatment of lignocellulosic biomass is crucial for efficient subsequent enzymatic hydrolysis and ethanol fermentation. in this study, wet explosion (wex) pretreatment was applied to cocksfoot grass and pretreatment conditions were tailored for maximizing the sugar yields using response surface methodology. the wex process parameters studied were temperature (160-210 °c), retention time (5-20 min), and dilute sulfuric acid concentration (0.2-0.5 %). the pretreatment parameter set e, apply ...201323709291
description of lactobacillus iwatensis sp. nov., isolated from orchardgrass (dactylis glomerata l.) silage, and lactobacillus backii sp. nov.two bacterial strains, designated iwt246(t) and iwt248, were isolated from orchardgrass (dactylis glomerata l.) silage from iwate prefecture, japan, and examined for a taxonomic study. both organisms were rod-shaped, gram-stain-positive, catalase-negative, facultatively anaerobic and homofermentative. the cell wall did not contain meso-diaminopimelic acid and the major fatty acids were c18 : 1ω9c and c19 cyclo 9,10/:1. comparative analyses of 16s rrna, phes and rpoa gene sequences revealed that ...201323687059
[effects of drought stress on the root growth and photosynthetic characters of dactylis glomerata seedlings].taking the drought-sensitive dactylis glomerata line "01998" and drought-tolerant cultivar "baoxing" as test materials, a pot experiment was conducted to study the effects of drought stress on the seedlings root growth, physiological characteristics, and leaf photosynthesis, aimed to approach the differences in the drought-tolerance mechanism of different d. glomerata lines (cultivars). under drought stress, the root vitality and root number of "01998" and "baoxing" presented a trend of increase ...201324483068
traditional uses of some medicinal plants in malatya (turkey).this study has identified not only the wild plants collected for medical purposes by local people of malatya province in the eastern anatolia region, but also the uses and local names of these plants. it tried to provide a source for researchers studying in ethnobotany, pharmacology and chemistry by comparing the information obtained from traditionally used herbs with previous laboratory studies.201323333750
plants suppress their emission of volatiles when growing with conspecifics.plant volatiles mediate interactions with herbivores, herbivore enemies, and abiotic stresses, but these interactions mostly have been studied with individual isolated plants. it is not yet known how intra- and interspecific plant competition influence volatile emission. in a greenhouse experiment, we investigated the volatile emission by red clover (trifolium pratense) growing alone, with a conspecific, or with an individual of the naturally co-occurring orchard grass, dactylis glomerata. the i ...201323525954
effect of supplementing orchardgrass herbage with a total mixed ration or flaxseed on fermentation profile and bacterial protein synthesis in continuous culture.a 4-unit dual-flow continuous culture fermentor system was used to evaluate the effects of supplementing fresh herbage with a total mixed ration (tmr) or flaxseed on nutrient digestibility, fermentation profile, and bacterial n synthesis. diets were randomly assigned to fermentors in a 4 × 4 latin square design. each fermentor was fed a total of 70 g of dry matter/d of 1 of 4 diets: (1) 100% freeze-dried orchardgrass herbage (dactylis glomerata l.; herb), (2) 100% freeze-dried tmr (100tmr), (3) ...201323522677
storage characteristics, nutritive value, energy content, and in vivo digestibility of moist, large rectangular bales of alfalfa-orchardgrass hay treated with a propionic acid-based preservative.unstable weather, poor drying conditions, and unpredictable rainfall events often place valuable hay crops at risk. recent research with large round bales composed of alfalfa (medicago sativa l.) and orchardgrass (dactylis glomerata l.) has shown that these large-bale packages are particularly sensitive to spontaneous heating and dry matter (dm) losses, as well as other undesirable changes with respect to forage fiber, protein, and energy density. various formulations of organic acids have been ...201323415527
anaerobic methane production from five common grassland species at sequential stages of maturity.since grass will likely be a dominant feedstock for on-farm anaerobic digestion in northwest europe, changes in the chemical composition of five common grass species with advancing harvest date in the primary growth were investigated and related to specific ch(4) yields. the increase in fibre components with advancing harvest date had a negative impact on the specific ch(4) yield (253 and 225 nl ch(4) kg(-1) vs for 12 may and 7 july harvests, respectively), and this impact was similar across the ...201323131635
stomata are less responsive to environmental stimuli in high background ozone in dactylis glomerata and ranunculus acris.two mesotrophic grassland species, ranunculus acris and dactylis glomerata were exposed to a range of ozone treatments (16.2-89.5 ppb 24 h mean) and two watering regimes under naturally fluctuating photosynthetically active radiation (par), vapour pressure deficit (vpd) and temperature. stomatal conductance was measured throughout the experiments, and the combined data set (>1000 measurements) was analysed for effects of low and high ozone on responses to environmental stimuli. we show that when ...201323354156
the crystallographic structure of panicum mosaic virus (pmv).the structure of panicum mosaic virus (pmv) was determined by x-ray diffraction analysis to 2.9å resolution. the crystals were of pseudo symmetry f23; the true crystallographic unit cell was of space group p2(1) with a=411.7å, b=403.9å and c=412.5å, with β=89.7°. the asymmetric unit was two entire t=3 virus particles, or 360 protein subunits. the structure was solved by conventional molecular replacement from two distant homologues, cocksfoot mottle virus (cfmv) and tobacco necrosis virus (tnv), ...201323123270
relationship between water soluble carbohydrate content, aphid endosymbionts and clonal performance of sitobion avenae on cocksfoot cultivars.aphids feed on plant phloem sap, rich in sugars but poor in essential amino acids. however, sugars cause osmotic regulation problems for aphids, which they overcome by hydrolysing the sugars in their gut and polymerising the hydrolysis products into oligosaccharides, excreted with honeydew. aphids harbour primary bacterial endosymbionts, which supply them with essential amino acids necessary for survival. they also harbour secondary (facultative) endosymbionts (sfs), some of which have a positiv ...201323342134
the effect of five forage species on transport and transformation of atrazine and isoxaflutole (balance) in lysimeter leachate.a field lysimeter study with bare ground and five different ground covers was established to evaluate the effect of forage grasses on the fate and transport of two herbicides in leachate. the herbicides were atrazine (atr; 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) and isoxaflutole [ixf; 5-cyclopropyl-4-(2-methylsulfonyl-4-trifluormethyl-benzoyl)isoxazole], which has the commercial name balance (aventis crop science, strasbourg, france). the ground covers included orchardgrass (dacty ...201314674520
rast inhibition: a collaborative study on its suitability and reproducibility for the assay of cocksfoot pollen extracts.the suitability and reproducibility of the inhibition mode of the radioallergosorbent test (rast) as a method for the assay of allergen extracts was examined in a collaborative study involving seven laboratories. participants assayed five coded extracts of cocksfoot grass (dactylis glomerata) pollen, including three batches from one manufacturer, both by a common method and by their local methods of rast inhibition. for the versions of rast inhibition employed in this study the estimates of rela ...20133719934
changes in protein thiols in response to salt stress in embryogenic suspension cultures of dactylis glomerata l.the aim of the present study is to assess the rate of protein disulphide formation and the activity of nadph-dependent thioredoxin and glutaredoxin systems, responsible for the reverse reduction of protein and mixed protein-glutathione disulphides, in embryogenic suspension cultures of dactylis glomerata, subjected to salt stress. two concentrations of nacl previously established as enhancing (0.085 mol/l) and inhibiting (0.17 mol/l) somatic embryogenesis were used. the quantitative (by colour r ...201426019548
meat goat kids finished on alfalfa, red clover or orchardgrass pastures: carcass merit and meat quality.this experiment was conducted in 2005-2007 to evaluate carcass and chevon (goat meat) quality parameters when meat-goat kids (n=72) were finished on alfalfa (medicago sativa l; alf); red clover (trifolium pratense l.; rcg); or orchardgrass (dactylis glomerata l.; ogr) pastures. carcass conformation score was greater (p=0.08) when meat-goat kids were finished on alf compared to ogr with rcg intermediate. chevon meat samples from goats finished on the three pasture treatments did not differ in ash ...201425089787
fibre digestibility, abundance of faecal bacteria and plasma acetate concentrations in overweight adult mares.the purpose of the present study was to compare digestibility of grass hay, faecal and plasma volatile fatty acid (vfa) concentrations, and faecal bacterial abundance in overweight and moderate-condition mares. five overweight adult mixed-breed mares and five adult mixed-breed mares in moderate condition were housed individually and limit-fed orchard grass (dactylis glomerata) hay at 20 g/kg body weight (as fed) daily for 14 d. forage dm and fibre digestibility were determined using aoac methods ...201425191602
cocksfoot mottle virus coat protein is dispensable for the systemic infection.the sobemovirus genome consists of polycistronic single-stranded positive-sense rna. the first orf encodes p1, a suppressor of rna silencing required for virus movement. the coat protein (cp) is expressed from the 3' proximal orf3 via subgenomic rna. in addition to its structural role, the cp of some sobemoviruses has been reported to be required for systemic movement and to interact with p1. the aim of this study was to analyse the role of cocksfoot mottle virus (cfmv) cp in the suppression of ...201424495467
dose-dependent response of nitrogen stable isotope ratio to proportion of legumes in diet to authenticate lamb meat produced from legume-rich diets.this study investigated the dose-dependent response in lamb meat of stable nitrogen isotope ratio to the dietary proportion of legumes, and the ability of the nitrogen isotope signature of the meat to authenticate meat produced from legume-rich diets. four groups of nine male romane lambs grazing a cocksfoot pasture were supplemented with different levels of fresh alfalfa forage to obtain four dietary proportions of alfalfa (0%, 25%, 50% and 75%) for 98 days on average before slaughter (groups l ...201424444961
soil microbial community responses to antibiotic-contaminated manure under different soil moisture regimes.sulfadiazine (sdz) is an antibiotic frequently administered to livestock, and it alters microbial communities when entering soils with animal manure, but understanding the interactions of these effects to the prevailing climatic regime has eluded researchers. a climatic factor that strongly controls microbial activity is soil moisture. here, we hypothesized that the effects of sdz on soil microbial communities will be modulated depending on the soil moisture conditions. to test this hypothesis, ...201424743980
morphological and genetic characteristics of hybrid combinations of dactylis glomerata.six f1 populations derived from crosses among 4 orchardgrass (dactylis glomerata l.) cultivars were studied by morphological and simple sequence repeat molecular markers to test for hybrid vigor and a correlation between genetic distance and heterosis. heterosis was observed for days to length of culm, leaf traits, tiller numbers, etc. significant differences between obverse and inverse crosses were found for eleven traits. a cytoplasmic effect existed for the agronomic traits considered in this ...201424615091
fertilization of epichloe typhina stromata by mycophagous slugs.epichloë typhina, a fungal endophyte of cool season grasses, is heterothallic and an obligate out-crosser. in areas of endemism, its spermatia are moved between stromata of the two opposite mating types through egg-laying activities of botanophila flies. in western oregon, where the fungus was inadvertently introduced into seed-production fields of dactylis glomerata (= orchardgrass, cocksfoot), flies do not appear to be the sole vectors for e. typhina fertilization. here we examined the role of ...201424603831
hypersensitivity to aeroallergens in patients with nasobronchial allergy.aeroallergens are the most common causes of allergy.201424937928
efficacy of biosolids in assisted phytostabilization of metalliferous acidic sandy soils with five grass species.the role of sewage sludge as an immobilising agent in the phytostabilization of metal-contaminated soil was evaluated using five grass species viz., dactylis glomerata l., festuca arundinacea schreb., f. rubra l., lolium perenne l., l. westerwoldicum l. the function of metal immobilization was investigated by monitoring ph, eh and cd, pb, and zn levels in column experiment over a period of 5-months. grasses grown on sewage sludge-amendments produced high biomass in comparison to controls. a sign ...201424912245
influence of fresh alfalfa supplementation on fat skatole and indole concentration and chop odour and flavour in lambs grazing a cocksfoot pasture.we investigated the influence of the level of fresh alfalfa supplementation on fat skatole and indole concentration and chop sensory attributes in grazing lambs. four groups of nine male romane lambs grazing a cocksfoot pasture were supplemented with various levels of alfalfa for at least 60days before slaughter. perirenal fat skatole concentration was higher for lambs that consumed alfalfa than for those that consumed only cocksfoot. the intensity of 'animal' odour in the lean part of the chop ...201425089784
analysis of diversity and relationships among orchardgrass (dactylis glomerata l.) accessions using start codon-targeted markers.orchardgrass, or cocksfoot, is an important perennial forage grass worldwide. the comprehensive understanding of orchardgrass accessions will benefit germplasm collection and breeding progress, and it will enhance efforts to improve forage yield and quality. therefore, 24 novel, simple, polymorphic, and reliable start codon-targeted (scot) markers were used to analyze the diversity and genetic relationships among 95 orchardgrass accessions. in total, 273 polymorphic bands were detected with an a ...201425036346
effects of heat acclimation on photosynthesis, antioxidant enzyme activities, and gene expression in orchardgrass under heat stress.the present study was designed to examine the effects of heat acclimation on enzymatic activity, transcription levels, the photosynthesis processes associated with thermostability in orchardgrass (dactylis glomerata l.).the stomatal conductance (gs), net photosynthetic rate (pn), and transpiration rates (tr) of both heat-acclimated (ha) and non-acclimated (na) plants were drastically reduced during heat treatment [using a 5-day heat stress treatment (38/30 °c ‒ day/night) followed by a 3-day rec ...201425255756
genetic diversity and structure of natural dactylis glomerata l. populations revealed by morphological and microsatellite-based (ssr/issr) markers.dactylis glomerata l. is an important forage species in the mediterranean region, and in other regions with a similar climate. genetic material from 3 locations in north, central, and south greece was studied, using morphological traits, ssr, and issr molecular markers. morphological analysis revealed differences among the geographic locations studied for all morphological traits, except the number of reproductive tillers. moreover, the highest phenotypic variation was observed on the accessions ...201425036166
sensitization profile to allergens in patients using multi-test ii.introduction medical intervention in allergies has broadened its perspective, also focusing in the quality of life of patients. patients are instructed, before using pharmacotherapy agents, to avoid the causal agent. objective this study aims to analyze the sensitization profile of patients with allergic complaints and identify possible characteristics specific to each age group and gender. methods a descriptive cross-sectional study included data collected from medical records (from multi-test ...201425992129
the effect of high polyphenol oxidase grass silage on metabolism of polyunsaturated fatty acids and nitrogen across the rumen of beef steers.polyphenol oxidase (ppo) activity in red clover (trifolium pratense) has been reported to reduce both proteolysis and lipolysis, resulting in greater n use efficiency and protection of pufa across the rumen. although high levels of ppo have been reported in grasses such as cocksfoot (orchard grass; dactylis glomerata), no in vivo research has determined whether grass ppo elicits the same response as red clover ppo. to test the hypothesis that silage ensiled from grass with high levels of ppo pro ...201425349355
[the study of profile of hypersensitivity to pollen and fungal allergens in the moscow region].the profile of hypersensitivity to pollen and fungal allergens is an important element of common pattern of immune diseases needed for development of effective pharmaceuticals. the purpose of the study was to analyze the rate of detection of combined forms of hypersensitivity to pollen and fungal allergens (pollen of birch, hazel, cocksfoot, wormwood, fungi a. alternata, c. herbarum, r. nigricans, p. notatum, c. albicans, a. fumigatus) in the moscow region on the basis of data of scarification s ...201425338462
reference gene selection for quantitative real-time reverse-transcriptase pcr in orchardgrass subjected to various abiotic stresses.quantitative real-time reverse-transcriptase pcr (qrt-pcr) is a powerful tool for the measurement of gene expression; however, the accuracy of this approach depends on the stability of reference genes. the objective of the present study was to identify the stable reference genes in orchardgrass (dactylis glomerata l.), a principal cool-season forage grass in the world. ten candidate reference genes were selected in this study including atp-binding [abc], actin [actin], cyclophilin [cyp2], glycer ...201425307767
sublingual or subcutaneous immunotherapy for seasonal allergic rhinitis: an indirect analysis of efficacy, safety and cost.the standard of preventive care for poorly controlled seasonal allergic rhinitis (ar) is subcutaneous immunotherapy (scit) with allergen extracts, administered in a physician's office. as an alternative to scit, sublingual immunotherapy (slit) is now an option for patients with seasonal ar. oralair, a slit tablet containing freeze-dried allergen extracts of five grasses [cocksfoot (dactylis glomerata), meadow grass (poa pratensis), rye grass (lolium perenne), sweet vernal grass (anthoxanthum odo ...201424444390
soluble phenolic compounds in fresh and ensiled orchard grass (dactylis glomerata l.), a common species in permanent pastures with potential as a biomass feedstock.high-value coproducts can greatly improve the feasibility of utilizing plant feedstocks for biorefining and biofuel production. plant polyphenolics have potential application in the pharmaceutical and cosmetic industries. orchard grass varieties have been noted for accumulation of polyphenolic compounds, and the current study determined the soluble phenol profile and content in the orchard grass variety 'abertop'. hydroxycinnamates and flavonoids were monitored during the transition from vegetat ...201424341797
efficacy of the oral chemoattractant receptor homologous molecule on th2 cells antagonist bi 671800 in patients with seasonal allergic rhinitis.the inflammatory response in patients with seasonal allergic rhinitis (sar) is partly mediated by the prostaglandin d2 receptor chemoattractant receptor homologous molecule on t(h)2 cells (crth2).201424332218
application of image analysis for grass tillering determination.tillering is defined as the process of above-ground shoot production by a single plant. the number of grass tillers is one of the most important parameters in ecology and breeding studies. the number of tillers is usually determined by manually counting the separated shoots from a single plant. unfortunately, this method is too time-consuming. in this study, a new method for counting grass tillers based on image analysis is presented. the usefulness of the method was evaluated for five grass spe ...201526446128
optimization of scot-pcr reaction system in dactylis glomerata by orthogonal design.the effects of 5 factors (template dna, mg(2+), dntps, taq dna polymerase, and primer) on the polymerase chain reaction (pcr) were investigated to optimize the start codon targeted polymor-phism (scot)-pcr system of dactylis glomerata l., using an orthogo-nal design l16 (4(5)). a suitable scot-pcr system for d. glomerata was established; the 20 μl reaction volume contained 3.0 mm mg(2+), 0.2 mm dntps, 1.0 u taq dna polymerase, 0.2 μm primer, 20 ng tem-plate dna, and 2 μl 10x buffer. each factor ...201525966069
identifying differentially expressed genes under heat stress and developing molecular markers in orchardgrass (dactylis glomerata l.) through transcriptome analysis.orchardgrass (dactylis glomerata l.) is a long-lived, cool-season forage grass that is commonly used for hay production. despite its economic importance, orchardgrass genome remains relatively unexplored. in this study, we used illumina rna sequencing to identify gene-associated molecular markers, including simple sequence repeats (ssrs) and single nucleotide polymorphisms (snps), as well as heat stress-induced differentially expressed genes (degs) in two orchardgrass genotypes, 'baoxing' (heat ...201525894804
remediation and reclamation of soils heavily contaminated with toxic metals as a substrate for greening with ornamental plants and grasses.soils highly contaminated with toxic metals are currently treated as waste despite their potential inherent fertility. we applied edta washing technology featuring chelant and process water recovery for remediation of soil with 4037, 2527, and 26 mg kg(-1) of pb, zn and cd, respectively in a pilot scale. a high edta dose (120 mmol kg(-1) of soil) removed 70%, 15%, and 58% of pb, zn, and cd, respectively, and reduced human oral bioaccessibility of pb below the limit of quantification and that of ...201525577699
[about minimization of expenses on allergy diagnosis in children: analysis of consistency of in vitro- and in vivo-allergic examinations results].high morbidity rate of atopic diseases among children, including high importance of grass pollen as a sensitizing agent, determine the relevance ofstudies on diagnostic examination systems for appointment of adequate therapy. the research of the most relevant allergens for patients to excludeof duplicating and uninformative tests became urgent after development of a new type of diagnostic tests that does not require expensive equipment. the objective of this research was to evaluate the results ...201527093804
content of lead and cadmium in aboveground plant organs of grasses growing on the areas adjacent to a route of big traffic.the effect of traffic on the content of lead and cadmium in grass morphological parts-leaves, shoots, and inflorescences-was studied. the samples were taken on a part of the european route e30 (siedlce by road). the following plants were tested: dactylis glomerata, arrhenatherum elatius, and alopecurus pratensis. during the flowering of grasses, the plant material was collected at distances of 1, 5, 10, and 15 m from the edge of the road, on the strip of road with a length of 9 km. in the collec ...201525292297
the fungal endophyte epichloë typhina improves photosynthesis efficiency of its host orchard grass (dactylis glomerata).according to the results presented in this paper the fungal endophyte epichloë typhina significantly improves the growth, psii photochemistry and c assimilation efficiency of its host dactylis glomerata. in this paper, we present a comprehensive study of the impact of the endophytic fungi epichloë typhina on its plant hosts' photosynthesis apparatus. chlorophyll a fluorescence, gas exchange, immuno-blotting and spectrophotometric measurements were employed to assess photosynthetic performance, c ...201526059605
exacerbation of atopic dermatitis on grass pollen exposure in an environmental challenge chamber.it has frequently been speculated that pruritus and skin lesions develop after topical exposure to aeroallergens in sensitized patients with atopic dermatitis (ad).201526044854
efficacy and safety of a novel nasal steroid, s0597, in patients with seasonal allergic rhinitis.allergic rhinitis (ar) poses a significant global burden with increasing prevalence. although intranasal glucocorticosteroids are effective, older agents can have limiting side effects. s0597, a novel intranasal glucocorticosteroid, has demonstrated good safety and tolerability during preclinical and phase 1 studies.201526272281
genetic evidence for reproductive isolation among sympatric epichloë endophytes as inferred from newly developed microsatellite markers.reproductive isolation is central to the maintenance of species, and especially in sympatry, effective barriers to prevent interspecific crosses are expected. host specificity is thought to constitute an effective mechanism for the formation of barriers in different genera of fungi, but evidence for endophytes is so far lacking. sexual epichloë species (ascomycota, clavicipitaceae) represent an ideal study system to investigate the mechanisms underlying speciation as mediated by host specificity ...201525542204
phytoremediation of polycyclic aromatic hydrocarbons in soils artificially polluted using plant-associated-endophytic bacteria and dactylis glomerata as the bioremediation plant.the reaction of soil microorganisms to the contamination of soil artificially polluted with polycyclic aromatic hydrocarbons (pahs) was evaluated in pot experiments. the plant used in the tests was cock's foot (dactylis glomerata). three different soils artificially contaminated with pahs were applied in the studies. three selected pahs (anthracene, phenanthrene, and pyrene) were used at the doses of 100, 500, and 1000 mg/kg d.m. of soil and diesel fuel at the doses of 100, 500, and 1000 mg/kg d ...201526638532
[carbon source utilization characteristics of soil microbial community for apple orchard with interplanting herbage].as soil fertility in apple orchard with clean tillage is declined continuously, interplanting herbage in orchard, which is a new orchard management model, plays an important role in improving orchard soil conditions. by using biolog micro-plate technique, this paper studied the functional diversity of soil microbial community under four species of management model in apple orchards, including clear tillage model, interplanting white clover model, interplanting small crown flower model and interp ...201526911017
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