Publications

the pigments of butterflies' wings: occurrence of the pigment of melanargia galatea in dactylis glomerata.		1926	16743736
the wax constituents of forage grasses: cocksfoot and perennial ryegrass.		1931	16744781
the phosphatides of forage grasses: cocksfoot.		1932	16744943
the glyceride fatty acids of forage grasses: cocksfoot and perennial ryegrass.		1932	16744806
the isolation of carotene and sterols from the unsaponifiable matter of cocksfoot.		1936	16746034
the composition of forage crops: cocksfoot. changes in the herbage during growth, with and without additions of nitrogenous fertilizer.		1939	16747022
increased meiotic irregularity accompanying inbreeding in dactylis glomerata l.		1943	17247095
effects of cytoplasm and gene dosage on expression of male sterility in dactylis glomerata.		1946	21066350
studies on the origin of dactylis glomerata l.		1948	18903868
chlorophyll lethal in natural populations of the orchard grass (dactylis glomerata l.). a case of balanced polymorphism in plants.		1961	13683843
grass pollen allergens. iv. the isolation of some of the principal allergens of phleum pratense and dactylis glomerata and their sensitivity spectra in patients.		1962	13863259
the energy value of ryegrass and cocksfoot assessed by a slaughter technique with lambs.		1965	5835395
metabolic inhibitor(s) in fractions of orchardgrass (dactylis glomerata l.) detected by in vitro rumen fermentation technique.		1967	6024807
metabolic inhibitor(s) in orchardgrass (dactylis glomerata l.).		1968	5690265
[studies on the physiological specialization of erysiphe graminis dc : iv. the host plants of cocksfoot mildew].	in the described experiments the interaction of 460 grass species with a mildew population obtained from dactylis glomerata l. 'motterwitzer' was examined. 420 species remained free of symptoms, while 40 species were susceptible. the most susceptible ones were dactylis aschersoniana graebner, dactylis glomerata l., dactylis glomerata spp. aschersoniana (graebn.) thell., and dactylis polygama horvat, but all showed differences in their behaviour. the unexpectedly low susceptibility of dactylis wo ...	1970	24435668
	leptopterna dolobrata (l.) (holarctis) and calocoris roseomaculatus (de geer) (palaearctis) are single-brooded in the whole range of their distribution and hibernate in the egg stage. both species are active only for 21/2 months, while the egg stage lasts over 9 months.the main food plants of leptopterna are dactylis glomerata and alopecurus pratensis. whereas the instars i-iii feed on the ears of the grasses only, the later stages, including the adults, may change to the stems and leaves. in th ...	1971	28311800
leaf microbodies (peroxisomes) and catalase localization in plants differing in their photosynthetic carbon pathways.	the tropical grasses sugarcane (saccharum officinarum) and pangolagrass (digitaria decumbens) contained fewer leaf microbodies than temperate orchardgrass (dactylis glomerata). leaf microbodies were seen in both the mesophyll and bundle sheath cells of tropical grasses. the fibrous elements in the microbodies of tropical grasses differed from those of the temperate grass. catalase was predominantly localized in the microbodies of leaf cells (3,3'-diaminobenzidine method). the site of greatest ca ...	1971	24493219
[enzootic calcinosis in cattle. 5. experimental studies on rabbits selectively fed with ball-grass (dactylis glomerata), gold-oats (trisetum flavescens) and a grass mixture].		1972	4551838
cocksfoot mild mosaic virus--phleum mottle virus: a comparison.		1974	4851066
cutaneous reactions to pollen extracts in patients with hay fever. special references to grass pollen recommended for treatment in fixed combination.	skin reactivity to 8 common grass pollens was investigated in patients with hay fever using commercial, unfractionated extracts in serial dilution technique. the highest degree of sensitivity was observed for cocksfoot, meadow fescue, meadow foxtail, rye grass, timothy and yorkshire fog. five of those are included in the allpyral 5-grass mix. the cutaneous responses to rye grass pollen overlap with those to timothy in a high degree.	1975	1173992
examination of cocksfoot pollen (dactylis glomerata) extracts by skin prick test in man, rast-based allergen assay and protein content.	three commercial preparations of cocksfoot pollen (dactylis glomerata) from different manufacturers were compared by skin prick tests in man, allergen activity as measured by the rast test and protein nitrogen unit (pnu) and total nitrogen (n) content. the skin prick tests were made on the forearm with the undiluted preparations and also with solutions of 1/5 and 1/25 dilutions. three groups each of 9 subjects were tested and the sites for the 9 tests were determined by 3 different latin squares ...	1975	1149937
rast, skin and nasal tests and the history in grass pollen allergy.	a comparison of the rast (phadebas), skin prick test, nasal test and a history of hay fever was made in 141 subjects, eighty-seven being prick test positive, with three concentrations of extracts of sweet vernal (anthoxanthum odoratum), cocksfoot (dactylis glomerata), meadow fescue (festuca elatior), rye (lolium perenne), timothy (phleum pratense), meadow (poa pratensis). in adequate concentration any of the species extracts would have sufficed for skin tests, with cocksfoot pollen the most pote ...	1975	1053442
[decomposition of grass-fructosanes by lactobacilli from silage (author's transl)].	lactobacilli isolated from grass silage were shown to belong to lb. plantarum, lb. casei and lb. brevis. in studies on the degradation of fructosanes significant differences among the three species were found. while all strains of l. casei were able to degrade fructosanes from dactylis glomerata or phleum pratense regardless of the degree of polymerization, no nember of lb. plantarum or lb. brevis could attack these polysaccharides.	1976	827143
demonstration of reaginic antibodies on human basophils by immune adherence to allergen-coated sepharose beads.	human basophils purified by gradient density centrifugation and differential glass bead adherence were interacted with grass pollen allergen in particulate form prepared by covalent coupling of dactylis glomerata protein concentrate to sepharose beads. basophils from allergic subjects were found to interact specifically with the allergen-coated bead surface in a manner so highly characteristic that false negatives with basophils from non-allergic subjects or subjects with unrelated allergies wer ...	1976	963864
variations in the digestibility of trace elements (cu, zn and mn) during the first cycle of cocksfoot growth.	the variations of trace element availability was examined with 6 adult male sheep (texel) maintained in metabolic crates. they received cocksfoot cut each morning during the first vegetation cycle and offered ad libitum (10 % refused food), for 6 day periods. it appears from the resulting data that: -- the stage of cocksfoot development has a strong influence upon the digestibility and the retention of copper, zinc and manganese. --there is an almost perfect parallelism between the variations of ...	1977	606139
search for a correlation between familial allergy to cocksfoot pollen and hla antigens.	twelve families comprising 62 individuals, 31 allergic to grasses, were examined. no significant correlation between pollinosis and the hla system was observed. however, there was a close correlation between cocksfoot skin tests and the rast (p less than 10(-6)).	1977	876127
enzyme determination and rast inhibition assays for orchard grass (dactylis glomerata): a comparison of commericial pollen extracts.	pollens release enzymes when they are dropped into water. orchard grass extracts were tested by two methods. the api-zym system determined 19 enzymes and colorimetric amounts of leucine aminopeptidases, acid phosphatase and trypsin. these were compared with rast inhibition assays. a good correlation was shown between the techniques. thus the enzyme dosage may be useful for the standardization of pollen extracts.	1978	686510
cellular histamine release, specific and total serum ige levels in hay fever patients and controls.	the amount of histamine released from blood leucocytes by allergen, the amount of allergen required to release 50% histamine and the total ige and ige specific for dactylis glomerata are compared in nine hay fever patients and five control individuals. the variation with time of total ige, of specific igee and of the percentage of histamine released at a fixed allergen concentration, corresponding to the cellular sensitivity, is shown for one control individual with positive in vitro and skin te ...	1978	77195
allergenic activity of fractions of cocksfoot (dacylis glomerata) pollen.	a cocksfoot (dactylis glomerata) pollen extract has been fractionated by isoelectric focusing in polyacrylamide gel, the separated components extracted from the gel and assayed by three methods: prick tests in human skin, rast and pca tests in monkey skin. prick testing in human skin showed that subjects responded differently to the separated components, indicating the presence of more than one antigenic determinant, and a profile of antibody activity against each fraction could be constructed. ...	1978	414856
fractionation of cocksfoot (dactylis glomerata) pollen by preparative isoelectric focussing.	the technique of isoelectric focussing in polyacrylamide gel has been developed to a preparative mode. using the improved technique cocksfoot (dactylis glomerata) pollen has been split into many components, and the allergenic reactions of the preparations have been studied by passive transfer in animal skin and by rast.	1978	23399
species specific grass pollen sensitivity: diagnosis and treatment with single grass species allpyral vaccines.	skin test titrations and nasal provocation tests in sixty patients with hay fever showed specific reactions to extracts of individual grass species. there was, however, no correlation between skin and nasal sensitivity. repeat testing after treatment with allpyral vaccines consisting of only the grass species to which the nasal reaction was most severe, or only one of several pollens to which reactions were equally severe, showed marked diminution of skin and nasal sensitivity not only to the si ...	1979	498489
an anomalous effect of allergen concentration on weal size elicited by skin testing for immediate hypersensitivity.	we report the finding of anomalous responses in the skin of atopic individuals when prick tested with serial dilutions of cocksfoot grass pollen extracts. each pollen extract was standardized using rast inhibition activity related to the who standard for ige. 75% of the patients gave a larger weal area with an allergen concentration of 1:10(5) than with the next higher concentration, i.e. 1:10(4). possible mechanisms whereby a larger skin weal is elicited with a lower concentration of allergen a ...	1979	421334
[reduced allergen immunotherapy of grass pollinosis (author's transl)].	an excellent model for investigating the maximization of specific immunotherapy is grass pollen allergy. treatment is more effective when the number of component antigens used are reduced. we know that rye and cocksfoot pollen carry major antigens of grass pollen. a controlled clinical study was carried out with 128 patients divided into 5 groups as follows: group 1 - treatment with a mixture of 7 grass pollen extracts (allpyral). group 2 - treatment with a cocksfoot pollen extract (allpyral). g ...	1980	6936982
the relationship of certain tymoviruses assessed from the amino acid composition of their coat proteins.	the amino acid composition of the coat proteins of the following viruses is reported: andean potato latent, clitoria yellow vein, desmodium yellow mottle, dulcamara mottle, eggplant mosaic, okra mosaic, ononis yellow mosaic, scrophularia mottle, and, as controls, cocksfoot mild mosaic and cocksfoot mottle viruses. these data, together with some already published, were used to compute classifications of the tymoviruses. these classifications show a general similarity to koenig's serological class ...	1980	7372445
ige antibody formation in balb/c mice without adjuvant: induction of responses to grass pollen extract and to a hapten-carrier conjugate.	the ige antibody responses of inbred balb/c mice to subcutaneously injected antigens, given without adjuvant, were studied. cocksfoot grass pollen extract (gpe) (100-100,000 pnu) induced no detectable primary ige antibody responses but following second and subsequent injections, substantial secondary responses were produced. weekly injections for 9 weeks of doses of doses of 0.1-10,000 pnu gpe all produced similar ige antibody titres as measured by 24 h passive cutaneous anaphylaxis (pca) in rat ...	1980	7380480
mineral status of beef cows and sheep on spring pasture fertilized with kieserite.	the utilization of minerals in orchardgrass (dactylis glomerata l.) fertilized with kieserite (2,240 kg/ha) was examined in spring with lactating beef cows and wether lambs. kieserite increased the concentration of mg in vegetative herbage from .22 to .29% ( p less than .01) and of s from .20 to .29% (p less than .01), with little effect on other minerals. fertilization also increased (p less than .01) apparent absorptions of mg and s by lambs fed cut orchardgrass, from 24.5 to 36.5% and from 58 ...	1981	6263852
grass pollen hyposensitization versus placebo therapy. i. clinical effectiveness and methodological aspects of a pre-seasonal course of desensitization with a four-grass pollen extract.	the present work reports the results of a double-blind clinical trial, comparing the effects of hyposensitization treatment versus placebo in 33 patients with allergic rhinitis, sensitive to a crude extract of the pollen of four different grasses (dactylis glomerata, lolium perenne, secale cereale, and phleum pratense). the distribution of these patients in the two groups was done randomly and gave two comparable groups, as far as clinical and biological features are concerned. the treatment cou ...	1981	7032341
allergenic activity of fractions of cocksfoot (dactylis glomerata) pollen. definition of active components by skin testing, and inhibition rast.	with the combined use of skin tests, rast and isoelectric focusing, a potency profile of a cocksfoot ((dactylis glomerata) pollen extract was prepared. it was found that two fractions with pi 4.5 and 6.0 were the most allergenic, had the highest protein concentration and were also the most easily extracted. the importance of converting mean weal sizes into potency units when comparing the allergenicity of pollen fractions is emphasized.	1981	7249343
successful crosses between festuca arundinacea schreb. and dactylis glomerata l.	five f1 plants have been obtained after extensive crossing between different ecotypes or varieties of festuca arundinacea schreb. and dactylis glomerata l. the success did not appear to depend on specific treatments (spraying with ε-aminocaproic acid or gibberellic acid or pre-pollination with killed pollen from the seed parent), but the crossability is limited to exceptional plants.f1 hybrids showed characteristics of both the parents. in four hybrids various developmental disturbances were obs ...	1981	24276635
embryoid and plantlet formation from leaf segments of dactylis glomerata l.	the purpose of this investigation was to demonstrate callus induction and plantlet formation from cultured leaf segments of 12-15 week-old dactylis glomerata l. (orchardgrass) plants. flat half-leaf sections, approximately 2-3 mm square, from the three innermost (youngest) leaves were isolated and individually plated serially beginning at the leaf base on a solid sh medium containing 30 μm of 3,6-dichloro-oanisic acid (dicamba). callus formed on leaf sections from all 50 plants used in the study ...	1982	24270764
heterogeneity of grass pollen allergens (dactylis glomerata) recognized by ige antibodies in human patients sera by a new nitrocellulose immunoprint technique.	a new nitrocellulose immunoprint technique has been developed to detect specific antigens or/and allergens present among a heterogeneous solution such as a water-soluble crude extract of a grass pollen (dactylis glomerata). the antigens are separated by isoelectric focusing (ief) in an agarose gel and characterized by their isoelectric point (pi). these antigens are transferred and immobilized on a nitrocellulose sheet. they are recognized by the binding of specific antibodies contained in an un ...	1982	6759375
performance of dairy cows in midlactation fed high quality grass pasture and concentrate at three percents of energy requirements.	thirty multiparous cows rotationally grazed high quality grass pastures (predominantly dactylis glomerata and poa pratensis) for 140 days. stage of lactation was 54 to 192 days at beginning of trial. cows were offered concentrates to supply 33, 66, or 100% of requirements for net energy for lactation above maintenance; daily intakes by the three groups averaged 3.5, 7.7, and 11.5 kg. refusal of concentrate tended to increase with supplementation. average milk production was 19.2, 20.4, and 21.5 ...	1983	6841749
inhibition of the passive cutaneous anaphylaxis reaction to dactylis glomerata pollen allergens by a purified component of this pollen.	by isoelectric focusing a protein fraction of pi 4.6 was isolated from a crude water-soluble extract of dactylis glomerata pollen (se). this fraction was neither immunogenic nor allergenic in balb/c mice. in one week, this protein inhibited the mouse ige-specific antibodies to the soluble extract as measured by pca in rats and was therefore called dactylis inhibitory protein (dip). two experimental approaches which lowered ige anti-se titer were undertaken. pretreatment with dip as well as injec ...	1983	6885115
investigations of the allergens of cocksfoot grass (dactylis glomerata) pollen.	the pollen of cocksfoot grass (dactylis glomerata) is an important cause of allergic reactions in man. preliminary studies, which established that constituents of an extract of this pollen could be separated, and then recovered efficiently, by size-exclusion chromatography on tsk g3000 sw, have been extended. a comparative examination has been made by this procedure of cocksfoot pollen extracts from different sources and of several batches of extract from one source. the recovery and distributio ...	1983	6630355
alkaloids in ergot found on different gramineae in the netherlands.	the alkaloid content and the composition of the alkaloid complex of thirteen samples of ergot sclerotia from different gramineous host species collected in the netherlands were investigated. two samples collected in france were also examined. ergot of glyceria fluitans (l.) r.br. did not contain alkaloids. the total alkaloid content of ergot found on the other wild grasses was more than 0.2%. the ratio between the contents of water soluble and water insoluble alkaloids of the investigated ergot ...	1983	6646990
a report on the consumption, composition and nutritional adequacy of a mixture of lush green perennial ryegrass (lolium perenne) and cocksfoot (dactylis glomerata) fed ad libitum to thoroughbred mares.	non-pregnant thoroughbred mares were stabled and subjected to 2 trials, each 24 h in duration, to establish their total consumption of a mixture of freshly cut, lush green perennial ryegrass (lolium perenne) and cocksfoot (dactylis glomerata) in approximately equal proportions; and to compare the total intake of crude protein, calcium, phosphorus and mass of the grass mixture on a dry matter basis with their daily nutritional requirements. the body mass of each mare was calculated at the commenc ...	1983	6655653
direct embryogenesis from mesophyll cells of orchardgrass.	segments taken from the basal 15 to 20 millimeters of the two innermost leaves of an orchardgrass (dactylis glomerata l.) genotype produced somatic (nonzygotic) embryos directly from mesophyll cells without an intervening callus when cultured on an agar medium with 30 micromolar 3,6-dichloro-o-anisic acid (dicamba). this demonstration of high-frequency embryogenesis from mesophyll cells in gramineae is strong evidence for totipotency of the cells.	1983	17753478
rooting pattern and water relations of three pasture grasses growing in drying soil.	seedlings of perennial ryegrass (lolium perenne l. s23), cocksfoot (dactylis glomerata l. s37) and timothy (phleum pratense l. s48) were rooted into tubes of soil and plants were either watered well or remained unwatered for a period of seven days. measurements were made of root water relations and of growth of roots and shoots. root turgor of dactylis was larger than that of lolium and phleum. as a result of accumulation of solutes, turgor of dactylis and lolium was maintained as the soil dried ...	1983	28310581
partition of membrane particles in aqueous two-polymer phase system and its practical use for purification of plasma membranes from plants.	a simplified method for the isolation of a plasma membrane-enriched fraction from plants utilizing an aqueous two-polymer phase system is outlined. mainly, the plant used was orchard grass (dactylis glomerata l.). the two-phase system consisted of 5.6% (w/w) of dextran t500 and 5.6% (w/w) of polyethyleneglycol 4000 in 0.5 molar sorbitol-15 millimolar tris-maleate (ph 7.3), and 30 millimolar nacl. in this system, the plasma membranes and the other membranes were preferentially partitioned into th ...	1983	16662942
wide hybridization experiments in cereals.	wide hybridization is a useful tool in plant breeding, but little is known about its possible range. for the cereals, wheat, barley and rye, this was tested with 15 different species of the poaceae and panicoideae. embryo formation could be obtained with agropyron repens, alopecurus agrestis, dactylis glomerata, festuca glauca, hordeum bulbosum, lolium perenne, pennisetum americanum, and zea mays. as well, haploid as diploid embryos occurred. new embryo culture techniques should enable these emb ...	1984	24257639
a collaborative study on rast inhibition using a common method.	a collaborative study was carried out to determine the reproducibility of the inhibition form of the radioallergosorbent test (rast). seven laboratories were provided with three coded extracts of cocksfoot (dactylis glomerata) pollen. all laboratories used a common method of rast inhibition and were supplied with all reagents required to carry out the assays. in addition, four laboratories used local methods of rast inhibition. potency estimates were in reasonable agreement between laboratories ...	1984	6697468
studies on freezing injury of plant cells: i. relation between thermotropic properties of isolated plasma membrane vesicles and freezing injury.	the thermotropic transition of plasma membrane of dactylis glomerata was studied by using fluorescence polarization of embedded fluorophore, 1,6-diphenyl-1,3,5-hexatriene. under the presence of 35% ethylene glycol, reversible thermotropic transitions were observed in isolated plasma membrane vesicles in nearly the same temperature range as the temperature of freezing injury to cells. in liposomes prepared from isolated plasma membranes, however, the thermotropic transitions occurred at much lowe ...	1984	16663597
protein and lipid compositions of isolated plasma membranes from orchard grass (dactylis glomerata l.) and changes during cold acclimation.	the chemical composition of plasma membrane fractions isolated from orchard grass seedlings (dactylis glomerata l.) was analyzed and compared with endomembranes. the plasma membrane is characterized by an enrichment of sterols and a lower degree of unsaturation of phospholipids. steryl glycosides were found to be one of the lipid components of the plasma membrane, but steryl esters and galactolipids were barely detectable. diphosphatidyl glycerol was characteristically detected in the mitochondr ...	1984	16663596
immunotherapy with alginate-conjugated and alum-precipitated grass pollen extracts in patients with allergic rhinoconjunctivitis.	in order to compare the safety and the efficacy of two grass pollen extracts made from the same starting material, i.e. equal proportions of cocksfoot and timothy, a 3-year study was made on patients with hay fever. one product was a dialysed aqueous extract of pollens which was chemically conjugated to activated sodium alginate ( conjuvac ). the other was a pyridine-extracted alum-precipitated crude extract (allpyral). forty patients with classical hay fever were admitted to the study. all gave ...	1984	6731753
presence of il 1 activity in the supernatant of human monocytes challenged with purified allergenic constituents.	purified allergenic constituents from dermatophagoides farinae mite (df 11 and df 6) and from orchard grass (dactylis glomerata) pollen (f 34) are able to trigger, in the absence of ige and igg antibodies, human adherent cells, leading to the secretion of interleukin 1, (il 1). this secretion was observed with adherent peripheral blood mononuclear cells from normal donors as well as from atopic patients. the il 1 activity was evaluated by the mice thymocyte mitogenesis in the presence of subopti ...	1984	6332080
isopycnic centrifugation of plant viruses in nycodenz density gradients.	isopycnic centrifugation of plant viruses in density gradients prepared from a new non-ionic medium (nycodenz) was investigated. particle density of luteo-, tymo-, nepo-, cocksfoot mild mosaic, tobamo-, hordei-like, potex- and potyviruses in buffered solutions of nycodenz ranged between 1.23 and 1.28 g/ml and did not strictly reflect their nucleic acid and protein composition. isopycnic centrifugation of several partially purified viruses yielded preparations which were pure by electron microsco ...	1984	6520198
purification and characterization of a major allergen from dactylis glomerata pollen: the ag dg1.	we have isolated an allergen (ag dg1) from dactylis glomerata pollen which is recognized by the serum of 95% of human patients sensitive to d. glomerata pollen, as has been shown by the nitrocellulose immunoprint technique. after two successive purifications by preparative isoelectric focusing (ief), dg1 was characterized as a single band in analytical agarose ief with a pi of 5.9 and was found to display 3 bands by sodium dodecyl sulfate polyacrylamide gel with the respective molecular weights: ...	1985	2414230
identification of orchard grass (dactylis glomerata) pollen allergens following electrophoretic transfer to nitrocellulose.	orchard grass (cocksfoot) pollen extracts, fractionated by polyacrylamide gradient electrophoresis or sds gel electrophoresis were electroblotted onto nitrocellulose membranes and probed with sera from orchard grass pollen-allergic patients and 125i-anti-human ige. the ige-binding components of the pollen were detected by autoradiography. elution studies showed that allergens could be extracted immediately and continuously over a 3-hour period. two fractions of mws 28,000 and 30,000 could be det ...	1985	2411668
production of a monoclonal antibody against a major allergen of dactylis glomerata pollen (dg1).	a mouse monoclonal antibody was produced against the most frequent allergen ag dg1ief, purified from the dactylis glomerata grass pollen by isoelectric focusing. using the immunoprint technique, this monoclonal antibody detected ag dg1ief in d. glomerata pollen and some cross-reacting molecules in four other grass pollens. this hybridoma was grown in ascitic fluid. an immunosorbent was made from it and, by affinity chromatography, was used to purify monoclonal ag dg1m from the pollen extract. ag ...	1985	2408551
induction of a passive cutaneous anaphylaxis inhibitory factor in mice.	the administration of a purified protein from dactylis glomerata pollen, named dactylis inhibitory protein (dip), to balb/c mice results in the production of a heat-resistant seric factor that blocks the passive cutaneous anaphylaxis (pca) reaction in rat skin. this pca inhibitory factor (pca-if) was purified following different purification procedures and analyzed in sodium dodecyl sulfate polyacrylamide gel. amongst the array of bands detected it was possible to ascribe the inhibitory activity ...	1985	4055080
comparison between rush immunotherapy with a standardized allergen and an alum adjuved pyridine extracted material in grass pollen allergy.	rush immunotherapy with a standardized and lyophilized cocksfoot pollen extract was performed in twenty-three allergic subjects and compared with classical immunotherapy done with an alum adjuved, pyridine extracted cocksfoot pollen extract (fifteen subjects) and a control group of ten patients. the three groups were perfectly matched. clinical benefits were analysed by means of symptom scores. it was shown that rush immunotherapy gave significantly better results when asthma or rhinitis was con ...	1985	3888440
comparison of the stability of a mite and a pollen extract stored in normal conditions of use.	pollen extracts are stable in solutions containing 50% glycerol. the stability of dermatophagoides pteronyssinus (dp) extracts have not been determined. two lyophilised extracts (dp and cocksfoot pollen) were reconstituted in 50% glycerol and stored for up to 6 months at +4 degrees c. every day the extracts were placed at room temperature for 1 hr to mimic the natural use of allergen extracts by allergists. every 2 months the extracts initially prepared were compared to freshly reconstituted ext ...	1985	3978775
partial purification and properties of phleinase induced in stem base of orchardgrass after defoliation.	phleinase induced in stem base of orchardgrass (dactylis glomerata l.) after defoliation was partially purified with ammonium sulfate precipitation, deae-sephadex chromatography, gel filtration, and preparative polyacrylamide gel electrophoresis. the molecular weight of phleinase was 57,000 as determined by gel chromatography. the enzyme showed normal michaelis-menten kinetics and its k(m) value was 91 millimolar for phlein of mean degree of polymerization 60 as substrate. reaction velocity of t ...	1985	16664288
thermoinactivation of human ige: antigenic and functional modifications.	the thermoinactivation kinetics of ige were studied in experimental models revealing the antigenic properties and the basophil-sensitizing capacity of these immunoglobulins. a pool of human sera containing anti-dactylis glomerata (dg) ige was heated from 5 min up to 4 hr at 56 degrees. the ige antigenicity was tested by two polyclonal 125i-labelled anti-ige antibodies; one anti-ige was specific of the whole fc epsilon region, while the other had a specificity restricted to the d epsilon 2 domain ...	1986	2420711
flock size and habitat-dependent food and energy intake of foraging goldfinches.	during the breeding season goldfinches (carduelis carduelis l.) feed on milky ripe seeds of about 20 food plants. individual goldfinches joining a flock reduce the time spent vigilant with increasing flock size. therefore birds feeding in flocks get an increased intake of kernels per time unit. this was measured for five different food plants (dactylis glomerata (gramineae), knautia arvensis (dipsacaceae), senecio vulgaris, taraxacum officinale, tragopogon pratensis (compositae)). in large-sized ...	1986	28312098
utilization of magnesium and other macrominerals in sheep supplemented with different readily-fermentable carbohydrates.	sheep were used to determine the effects of dietary supplementation with readily-fermentable carbohydrates on mg, ca, k and p utilization. in each of two metabolism trials, 15 mature, crossbred wethers (average weight, 49.2 kg) were allotted to five dietary treatments consisting of 800 g/d of orchardgrass (dactylis glomerata, l.) hay alone, or supplemented with 450 g/d of either glucose, sucrose, lactose or starch. each trial consisted of a 5-d adjustment period, a 10-d preliminary period and a ...	1987	3443586
preliminary experimental and clinical results with inactivated allergens conjugated to the corynebacterium granulosum-derived immunomodulator p40.	allergoids have been used successfully for immunotherapy of allergic disorders. it has appeared to us that the effect of allergoids could be potentiated by their coupling to an immunomodulator. in the present study we show that a conjugate made up of the coupling of ovalbumin through glutaraldehyde action to the c. granulosum-derived immunomodulator p40 is completely devoid of antigenicity and of cross-reactivity with ovalbumin. this conjugate was found to significantly inhibit mast cell degranu ...	1987	3620123
[comparative study of various technics for measuring serum ige levels].	in this work we report the results of a comparative study of different techniques to measure specific ige in sera. three methods were used, phadebas rast, phadezym rast (pharmacia) and allergen-specific fast+ test (3m diagnostics): 127 sera were tested with several pneumoallergens (housedust, dermatophagoides pteronyssinus, dactylis glomerata pollen, cat and dog hair). analysis of the results shows the following correlations: phadebas and phadezym rast 92%; phadebas rast and fast+ 88%; phadezym ...	1987	3453730
effects of ambient levels of air pollution on grass swards subjected to different defoliation regimes.	the urgent need to evaluate effects of air quality on plant growth in the field is discussed in relation to the difficulties which field-based studies pose in the interpretation of data. an initial study is described which aimed to overcome some of these difficulties and the data are discussed within the remaining limitations. the productivity of pure swards of lolium perenne l. and dactylis glomerata l., subjected to defoliation at different times of the year, was studied at field sites with di ...	1987	15092760
polyploidy and habitat differentiation in dactylis glomerata l. from galicia (spain).	the microdistribution of diploid and tetraploid plants of dactylis glomerata l. was examined and related to their immediate environment in several sites in central galicia, where morphologically indistinguishable individuals of both ploidies grow in sympatry. the two related cytotypes differed in habitat preference. diploids were mainly confined to the low-density forest-floor habitat in woodlands of mostly ancient origin, whereas tetraploids were widespread in varied habitats but clearly predom ...	1987	28311527
chloroplast dna variation in the grass tribe festuceae.	six grasses, hordeum sativum, dactylis glomerata, festuca arundinacea, f. pratensis, f. rubra and lolium multiflorum were subjected to chloroplast dna analysis based on restriction endonuclease digestion fragments and end labeling with (35)s nucleotides. this method is compared with others in general use. the results indicate that lolium multiflorum is closely affiliated with festuca pratensis and f. arundinacea; in fact much closer than f. rubra is to any of them.	1987	24241665
ecological energetics of central european grasshoppers (orthoptera: acrididae).	biomass and energy budgets and food utilization efficiencies of laboratory and wild populations of three central european grasshopper species, chorthippus parallelus (zetterstedt), c. biguttulus (l.), and gomphocerus rufus (l.), were studied between 1979 and 1984. larval consumption is relatively low in c. biguttulus (c=4.3 kj/ind.) compared with c. parallelus and g. rufus (4.9 kj/ind.). in the adult phase (maturity) consumption rates of c. biguttulus and g. rufus (6.9 kj/ind.) are similar, but ...	1987	28310423
transgenic plants of orchardgrass (dactylis glomerata l.) from protoplasts.	we have demonstrated the transfer and expression of a foreign chimeric gene in the grass species, dactylis glomerata l. this species is a member of the gramineae sub-family pooideae, which includes the small grain cereals, from which transformed plants have not yet been obtained. a chimeric hygromycin-resistance gene was introduced into protoplasts isolated from an embryogenic suspension culture, using heat shock followed by electroporation or polyethylene glycol treatment. cell colonies resista ...	1988	24240393
inhibition of somatic embryogenesis in orchardgrass by endogenous cytokinins.	endogenous indoleacetic acid (iaa) and cytokinin concentrations were measured by high performance liquid chromatography in leaf sections of an orchardgrass (dactylis glomerata l.) genotype which exhibited a high capacity for somatic embryogenesis in vitro and in two genotypes that did not exhibit this capacity. the nonembryogenic genotypes contained 3- to 4-fold higher concentrations of zeatin, zeatin riboside, dihydrozeatin, dihydrozeatin riboside, and total cytokinins than the embryogenic geno ...	1988	16666493
plant regeneration from protoplasts of embryogenic suspension cultures of orchardgrass (dactylis glomerata l.).	an embryogenic suspension culture of orchardgrass (dactylis glomerata l.) consisting of small, embryogenic cell clusters was obtained from callus formed on basal sections of young leaves through a process of selective enrichment. these suspensions were used as a source of protoplasts. the isolated protoplasts divided at a frequency of 0.5-10% when plated in an agarose solidified culture medium. conditioned medium, in which embryogenic dactylis suspension cultures had been grown, was found to inc ...	1988	24240247
asymmetric small-scale distribution and allelopathy: interaction betweenrumex obtusifolius l. and meadow species.	analysis of small-scale distribution may allow allelopathic interaction among plant species to be distinguished from competitive interaction. if all environmental factors except slope are uniform, allelopathy produces spatial asymmetry in the association with a hypothetical receptor species and the corresponding hypothetical emitter species in the neighborhood of the latter. the interaction betweenrumex obtusifolius and the meadow specieslolium perenne, poa pratensis, dactylis glomerata, andtrif ...	1988	24276533
[identification of orchard grass (dactylis glomerata) pollen allergens by immunoblotting].		1988	3228359
study of the epitope structure of purified dac g i and lol p i, the major allergens of dactylis glomerata and lolium perenne pollens, using monoclonal antibodies.	the use of mab allowed us to further analyze the cross-reactivity between purified dac g i and lol p i, the major allergens of dactylis glomerata (cocksfoot) and lolium perenne (rye grass), respectively. it was first shown, using ief, followed by immunoprinting, that serum ige antibodies from most grass-sensitive patients recognize both dac g i and lol p i. second, three different anti-lol p i mab, 290a-167, 348a-6, and 539a-6, and one anti-dac g i mab, p3b2 were all shown to react with dac g i ...	1988	2460536
selection among nonlinear models for rate of passage studies in ruminants.	eight toggenburg wether goats were fed either coastal bermudagrass (cynodon dactylon) or orchardgrass (dactylis glomerata)-alfalfa (medicago sativa) hay (57:43%) in a reversal design. four markers were used to study the movement of different digesta fractions: erbium for large particles greater than 1,700 microns; ytterbium for medium particles less than 1,700 microns and greater than 500 microns; chromium for small particles less than 500 microns and greater than 32 microns; and cobalt to follo ...	1988	3225248
effect of ruminal infusion of glucose, volatile fatty acids and hydrochloric acid on mineral metabolism in sheep.	two experiments were conducted to study the effects of alterations in ruminal ph and volatile fatty acid (vfa) concentrations on utilization of mg and other minerals. in exp. 1, two metabolism trials were conducted with 12 ruminally cannulated crossbred wethers fed 800 g/d of orchard-grass (dactylis glomerata, l.) hay. after each feeding, wethers were ruminally infused with 500 ml (4.2 ml/min) or either 1) deionized water, 2) 40% (w/v) glucose solution, 3) .26 m propionic and .17 m butyric acid ...	1988	3372390
cloning of cdna coding for an allergen of cocksfoot grass (dactylis glomerata) pollen.	messenger rna isolated from cocksfoot grass (dactylis glomerata) anthers has been used to generate a cdna library in lambda t11. three cdna clones (7.8, 8.1, and 8.3) were demonstrated to be recognized by human ige antibodies in atopic serum and by rabbit polyclonal antiserum raised to a crude aqueous extract of cocksfoot pollen. the size of the cdna inserts was determined as approximately 700 bp, and restriction mapping demonstrated them to be identical sequences. lysogens obtained in escherich ...	1989	2807580
the effects of intake of lucerne (medicago sativa l.) and orchard grass (dactylis glomerata l.) hay on the motility of the forestomach and digesta flow at the abomaso-duodenal junction of the sheep.	1. the relations between food intake, reticulo-ruminal motility and abomasal digesta outflow were investigated in ewes receiving lucerne (medicago sativa) v. orchard grass (dactylis glomerata) hay. abomasal digesta outflow was recorded continuously by means of an electromagnetic flowmeter probe inserted into a duodenal t-shape cannula and the motility using strain-gauge force transducers. volumes and turnover rates of both rumen and abomasal liquid phases were measured by credta dilution. 2. the ...	1989	2758019
characterization with monoclonal and polyclonal antibodies of a new major allergen from grass pollen in the group i molecular weight range.	we have purified a 24/25 kd allergen from orchard grass pollen (dactylis glomerata) that has an allergenic potency similar to that of the major group i allergen. we provisionally named this allergen grass 4b1 after the monoclonal antibody used for its identification and purification. this monoclonal antibody was obtained by immunizing mice with whole lolium perenne-pollen extract and by screening the antibody producing hybrids for reactivity with dactylis glomerata-pollen extract. grass 4b1 is p ...	1989	2913133
relationship between skin-prick tests, the multiple allergosorbent test and symptoms of allergic disease.	in 47 atopic subjects, skin-prick testing to 10 common allergens was performed, and specific ige to the same allergens was assessed by the multi-allergosorbent chemiluminescent assay (mast-cla). overall agreement between the tests was 66.4% for conventionally positive skin tests (weal diameter greater than or equal to 3 mm), rising to 78.5% when a positive skin test was defined as having a weal diameter greater than or equal to 5 mm. agreement between the tests was statistically significant for ...	1989	2649214
[skin reactivity to grass pollen and the mite dermatophagoides pteronyssinus in the prick-test using 2 types of allergen preparations].	the reliability of prick testing with allergen preparations produced in yugoslavia was assessed by comparison to the internationally accepted ones (pharmacia diagnostica) of 100,000 bu activity. the following allergens were used: the mite dermatophagoides pteronyssinus in 107 subjects, and grass pollens: dactylis glomerata, poa pratensis, phleum pratense and secale cereale in 39 subjects. the clinical significance of the response was assessed in relation to the skin reaction to the negative cont ...	1989	2638564
vegetation establishment on a deposit of zinc mine wastes.	field trials concerning the establishment of plant cover on a deposit of wastes from the ammeberg zinc mine in central sweden were carried out during 1976-1985. different soil conditioners and manures were applied and plant species cultivars were evaluated with regard to plant biomass, vigour, durability and content of zinc, lead and cadmium. sewage sludge and topsoil led to better establishment of grasses than did municipal waste, straw and hydraulic seeding. after 2 years, festuca rubra and po ...	1989	15092483
autopolyploidy in dactylis glomerata l.: further evidence from studies of chloroplast dna variation.	chloroplast dna variation has been used to examine some of the maternal lineages involved in the evolution of the intraspecific polyploid complex, dactylis glomerata l. diploid (2x) and tetraploid (4x) individuals were collected from natural populations of the subspecies glomerata (4x), marina (4x) and lusitanica (2x), as well as from sympatric 2x/4x populations of the galician type. digestion of their ctdna with 11 restriction endonucleases revealed enough variation to characterise three ctdna ...	1989	24227247
characterization of grass pollen-specific ige, iga, igm classes and igg subclasses in allergic patients.	water-soluble grass pollen extracts (dactylis glomerata) were separated by isoelectric focusing in a wide ph range (2-11) in agarose gel. after focusing, two successive gel prints were taken. the first one, on an ordinary nitrocellulose filter during 10 s, enabled the visualization of separated components of the pollen, after india ink staining. the second one, on a cyanogen bromide-activated nitrocellulose filter obtained after a 10-min transfer and saturation step, was incubated overnight with ...	1989	2759718
monoclonal antibodies to proteins from cocksfoot grass (dactylis glomerata) pollen: isolation and n-terminal sequence of a major allergen.	monoclonal antibodies (mabs) raised to an aqueous extract of cocksfoot grass (dactylis glomerata) pollen have been characterised. mab 1b9 was demonstrated by sds-page and western blotting to recognise a major allergen of an approximate molecular weight of 28 kd in this extract, termed dg3, and a component with a molecular weight of between 35 and 40 kd in an extract of secale cereale (cultivated rye) pollen. the 28 kd component of cocksfoot grass pollen isolated by affinity chromatography using ...	1990	2210876
additional hosts for the ring nematode, criconemella xenoplax.	some common legumes and weeds indigenous to peach orchards in south carolina were tested in greenhouse experiments to determine their host suitability for criconemella xenoplax. legumes that were hosts for the nematode were dwarf english trefoil (lotus corniculatus var. arvensis), big trefoil (l. uliginosis), birdsfoot trefoil (l. corniculatus), narrowleaf birdsfoot trefoil (l. tenuis), ball clover (trifolium nigrescens), rose clover (t. hirtum), subterranean clover (t. subterraneum), striate le ...	1990	19287692
detection of kestoses and kestose-related oligosaccharides in extracts of festuca arundinacea, dactylis glomerata l., and asparagus officinalis l. root cultures and invertase by c and h nuclear magnetic resonance spectroscopy.	a previous study (kl forsythe, ms feather [1989] carbohydr res 185: 315-319) showed that (13)c nuclear magnetic resonance spectroscopy can be used to detect and identify mixtures of 1-kestose and neokestose after conversion to the acetate derivatives. in this study, unequivocal assignments are made for the anomeric carbon and proton signals for the above two trisaccharide acetates as well as for 6-kestose hendecaacetate and for nystose tetradecaacetate (a 1-kestose-derived tetrasaccharide). a nu ...	1990	16667365
rast in diagnosis and therapy of allergic rhinitis.	skin tests, rast, nasal provocation tests, and other nasal allergy examinations were performed on 463 patients exhibiting symptoms of allergic rhinitis who were examined at the department of otolaryngology of osaka city university medical school, and comparisons were made between the rast scores and the results of the other examinations. a study was also made of the change in grade of the allergic rhinitis and the change of the rast score resulting from one year of immunotherapy. the rates of ag ...	1991	1842868
characterization of the major allergen of cynodon dactylon (bermuda grass) pollen, cyn d i.	an allergen from cynodon dactylon (bermuda grass) pollen, cyn d i, has been purified by a combination of concanavalin a-sepharose affinity chromatography, and carboxymethyl-sepharose chromatography. the allergen constitutes the major allergenic component of the pollen extract as observed by immunoelectrophoretic techniques, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, an ige-inhibition experiment, and skin testing. in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, cyn d ...	1991	1955635
cocksfoot grass pollen allergens and genetic of immune response.	the genetic basis of allergic response to grass pollen allergens of 23 portuguese families have been studied. the two or three generations families formed a total of 128 individuals including at least one parent and one child sensitive to dactylis glomerata grass pollen. hla class ii genes were studied by pcr amplification and rflp analysis. our population study has revealed a higher frequency of allele 2 of dob in the allergic population than in non allergic individuals. 60% of cocksfoot sensit ...	1991	1811648
genetic variation and covariation in a population of tetraploid dactylis l. accessions.	efficient utilization of divergent germ plasm sources in breeding cultivated dactylis glomerata l. ssp. glomerata domin depends on knowledge of quantitative variation within and among accessions. this study was undertaken to quantify variation and covariation for forage yield, maturity, disease reaction, and ground cover within a population of tetraploid dactylis accessions. variation was observed among families within the population for each variable. most genetic variation (73%-93% of the fami ...	1991	24221211
variability of crossreactivity of ige antibodies to group i and v allergens in eight grass pollen species.	crossreactivity to dactylis glomerata, festuca rubra, phleum pratense, anthoxanthum odoratum, secale cereale, zea mays, and phragmites communis of ige antibodies against lol p i or lol p v was investigated by means of rast-inhibition. within a group of sera the degree of crossreactivity was demonstrated to be highly variable. individual sera were not always equally crossreactive to all pollen species. a high degree of crossreactivity for group i allergens did not necessarily implicate the same f ...	1992	1382820
n-terminal amino acid sequence homologies of group v grass pollen allergens.	in an earlier study, we presented data regarding the immunoaffinity purification and n-terminal sequencing of a major pollen allergen from orchard/cocks-foot grass (dactylis glomerata), now identified as the group v allergen dac g v. in this paper, we have extended our investigations to include group v allergens from other grass species. our data confirm the presence of group v-restricted characteristic n-terminal amino acid sequences containing a high alanine and hydroxyproline (p') rather than ...	1992	1643443
[sensitivity and specificity of the determination of ige using chemiluminescence (cla allergy test)].	we studied 80 patients. all patients underwent a thorough clinical evaluation, including a comprehensive study and physical examination of the ear, nose, throat and chest. each patient underwent prick testing and a nasal provocation test. in addition, blood was drawn for the allergen-specific ige test (rast) and total serum ige levels. we selected 11 allergens for this study: grass (dactylis glomerata-orchard grass, phelum pratense-timothy grass, lolium-raygrass), weeds (chenipodium, parietaria) ...	1992	1509983
group v allergens in grass pollens: iv. similarities in amino acid compositions and nh2-terminal sequences of the group v allergens from lolium perenne, poa pratensis and dactylis glomerata.	monoclonal antibodies (ppv4) raised against phleum pratense group v allergen were used for immuno-affinity chromatography of cross-reacting group v allergens from related grass species. fractions enriched in group v allergen were obtained from lolium perenne, poa pratense and dactylis glomerata extracts. the major components in these fractions were found in the mwr range 25-28 kd. ige binding to these components was shown using a pool of grass allergic sera, by sds-page immunoblotting. these fra ...	1992	1611548
forage systems for beef production from conception to slaughter: i. cow-calf production.	six year-round, all-forage, three-paddock systems for beef cow-calf production were used to produce five calf crops during a 6-yr period. forages grazed by cows during spring, summer, and early fall consisted of one paddock of 1) tall fescue (festuca arundinacea schreb.)-ladino clover (trifolium repens l.) or 2) kentucky blue-grass (poa pratensis l.)-white clover (trifolium repens l.). each of these forage mixtures was combined in a factorial arrangement with two paddocks of either 1) fescue-red ...	1992	1548221