Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| effect of soil type and fertilization level on mineral concentration of pasture: potential relationships to ruminant performance and health. | a three-year study was conducted to measure the effects of varying levels of dairy slurry application on mineral concentration of forages from three soils types. slurry was applied to orchardgrass (dactylis glomerata [l.] cv. pennlate) growing in 60-cm diameter drainage lysimeters to measure the effect of four levels of slurry (urine and feces) n application (0, 168, 336, and 672 kg of n.ha-1.yr-1) on mineral (p, k, ca, mg) concentration of the forage on three soil types (hagerstown, hartleton, ... | 2003 | 12817509 |
| [in vitro effects of antiallergic eyedrops on complement activation induced by particulate matter]. | recent decades have been marked by an increasing number of patients suffering from ocular allergic-like symptoms without being associated with an increase in ige levels. these symptoms include heaviness of the lid, foreign body sensation, burning, stinging and photophobia. both epidemiological studies and controlled human exposure clinical studies have shown cause-effect relationships between allergic-like symptoms and environmental factors such as outdoor air pollutants or poor indoor air quali ... | 2003 | 12843888 |
| soil mixing to decrease surface stratification of phosphorus in manured soils. | continual applications of fertilizer and manure to permanent grassland or no-till soils can lead to an accumulation of p at the surface, which in turn increases the potential for p loss in overland flow. to investigate the feasibility of redistributing surface stratified p within the soil profile by plowing, mehlich-3 p rich surface soils (128-961 mg kg(-) in 0-5 cm) were incubated with lower-p subsoil (16-119 mg kg(-1) in 5-20 cm) for 18 manured soils from oklahoma and pennsylvania that had rec ... | 2003 | 12931893 |
| comparison of growth performance of lolium perenne l., dactylis glomerata l. and agropyron elongatum (host.) p. beauv. for erosion control in turkey. | this study was carried out in plastic containers to compare growth performances of perennial ryegrass, orchardgrass and tall wheatgrass to be given priority in revegetation studies in turkey. three pre-germinated seeds of each grass species were planted separately into the soil in the black plastic containers. seedlings were harvested 2, 4 and 6 months after planting pre-germinated seeds and measured for percentage of seedling emergence, rooting depth, height growth, leaf and tiller development ... | 2003 | 12974411 |
| human serum ige-mediated mast cell degranulation shows poor correlation to allergen-specific ige content. | although allergen-specific ige content in serum can be determined immunochemically, little is known about the relationship between this parameter and the strength of the degranulation response upon allergen triggering. | 2003 | 14510723 |
| the complete nucleotide sequence of subterranean clover mottle virus. | the complete nucleotide sequence of subterranean clover mottle virus (scmov) genomic rna has been determined. the scmov genome is 4,258 nucleotides in length. it shares most nucleotide and amino acid sequence identity with the genome of lucerne transient streak virus (ltsv). scmov rna encodes four overlapping open reading frames and has a genome organisation similar to that of cocksfoot mottle virus (cfmv). orf1 and orf4 are predicted to encode single proteins. orf2 is predicted to encode two pr ... | 2003 | 14579180 |
| [effects of grazing, cutting and decapitating on grass populations on the artificial grassland in subtropical zone of china]. | studies on the effects of grazing, cutting and decapitating on grass populations on the artificial grassland in the subtropical zone of china showed that moderate grazing and cutting could increase the adaptability of dactylis glomerata and lolium prenn due to decapitating. decapitating could rescind the apical dominance and reproduction growth of grass, and hence, the ecological effects of grazing and cutting on grass could be realized by decapitating, and the population density, caloric value ... | 2003 | 14655369 |
| vertical leaf nitrogen distribution in relation to nitrogen status in grassland plants. | vertical gradients of leaf nitrogen (n) per unit leaf area (nla) are viewed as plastic responses that optimize n utilization with respect to carbon assimilation. however, it has been shown that plant species, sowing density and n availability affect the steepness of the nla gradient relative to the photon flux density (pfd) gradient. this paper tests the hypothesis that such variation is related to the n status of the plant. the n status was analysed using the concept of the critical n concentra ... | 2003 | 12967909 |
| oxygen deficiency affects carbohydrate reserves in overwintering forage crops. | anaerobic conditions developing under an ice cover affect winter survival and spring regrowth of economically important perennial crops. the objective was to compare, during a prolonged period of low (<2%) o2 at low temperature, the concentration of carbohydrates of four plant species contrasting in their resistance to oxygen deficiency. four perennial forage species, lucerne (medicago sativa l.), red clover (trifolium pratense l.), timothy (phleum pratense l.), and cocksfoot (dactylis glomerata ... | 2003 | 12754268 |
| silica accumulation in triticum aestivum l. and dactylis glomerata l. | the silica accumulation in orchard grass (dactylis glomerata l.) and wheat (triticum aestivum l.) has been studied in plant samples grown under defined conditions in a pot trial. the plant habit and the quantity of biomineralised silica within the selected gramineae depend to a remarkable extent on the soil. the plants grew with different soil ph values and silica additives. by means of atomic absorption spectrometry, the silicon enrichment in different plant parts was determined. in dried plant ... | 2003 | 12698227 |
| effect of smoke, charred wood, and nitrogenous compounds on seed germination of ten species from woodland in central-western spain. | the effect of smoke, charred wood, and nitrogenous compounds on germination was tested on 10 species of the cistaceae, poaceae, fabaceae, and asteraceae, from fire-prone, shrubby woodlands in central-western spain. dry seeds were exposed to smoke, by watering with distilled water-charred wood suspensions, or nano2, kno3, nh4cl, and nh4no3. smoke enhanced germination in 9 of 10 of the species. in species of poaceae, germination was stimulated by 20 min of smoke exposure. in asteraceae and fabacea ... | 2003 | 12647865 |
| inferring the evolutionary history of rice yellow mottle virus from genomic, phylogenetic, and phylogeographic studies. | fourteen isolates of rice yellow mottle virus (rymv) were selected as representative of the genetic variability of the virus in africa from a total set of 320 isolates serologically typed or partially sequenced. the 14 isolates were fully sequenced and analyzed together with two previously reported sequences. rymv had a genomic organization similar to that of cocksfoot mottle sobemovirus. the average nucleotide diversity among the 16 isolates of rymv was 7%, and the maximum diversity between any ... | 2004 | 15016846 |
| analysis of liquid extracts from tree and grass pollens by capillary electromigration methods. | capillary electromigration methods, zone electrophoresis (cze), micellar electrokinetic chromatography (cmekc) and isotachophoresis (citp), have been used for analysis of water and water-buffer extracts from tree-common birch (betula verrucosa) and grass-orchardgrass (dactylis glomerata) pollen samples. water extracts were analyzed by cze using acetic acid as background electrolyte (bge), by cmekc in tris-phosphate bge with anionic detergent sodium dodecyl sulfate (sds) micellar pseudophase (tp- ... | 2004 | 15236695 |
| lactation performance of holstein cows fed fescue, orchardgrass, or alfalfa silage. | perennial grasses are increasingly being used as an integral part of nutrient management plans, but fescue (festuca arundinacea schreb.) is often overlooked because of perceived intake problems. a 30-d study was conducted to evaluate the lactation performance of cows fed a fescue silage-based total mixed ration (tmr) compared with orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.) silage-based tmr, when forages are harvested at recommended neutral detergent fiber (ndf) levels. ... | 2004 | 15328241 |
| characterization of natural dac g 1 variants: an alternative to recombinant group 1 allergens. | production of soluble correctly folded recombinant group 1 allergens has proven to be difficult. purified natural group 1 allergens could be an alternative for application in immunotherapy. | 2004 | 15536420 |
| surfactant protein d increases phagocytosis and aggregation of pollen-allergen starch granules. | recent studies have shown that surfactant components, in particular the collectins surfactant protein (sp)-a and -d, modulate the phagocytosis of various pathogens by alveolar macrophages. this interaction might be important not only for the elimination of pathogens but also for the elimination of inhaled allergens and might explain anti-inflammatory effects of sp-a and sp-d in allergic airway inflammation. we investigated the effect of surfactant components on the phagocytosis of allergen-conta ... | 2004 | 15591410 |
| [ecological strategies of soil microbial communities under plants of meadow ecosystems]. | dominant growth strategies of soil microbial communities of mown and unmown meadows were assessed with respect to the constants of saturation and maximal specific growth rate of microorganisms. the microbial community of mown-meadow soil was characterized by a greater biomass and activity due to prevalence of microorganisms with the r strategy, compared to the microbial community of unmown-meadow soil. in contrast to nonrhizosphere soil, rhizosphere soil was dominated by rapidly growing microorg ... | 2004 | 15615453 |
| sensitization to common allergens, especially pollens, among children with respiratory allergy in the trakya region of turkey. | asthma and allergic rhinitis are common problems in children and the causative pollen allergens vary according to the geographical area. the aim of this study was to investigate patterns of sensitization to common inhalant allergens, especially pollens, in turkish children living in the trakya region and to determine differences between rural and urban areas. allergen skin testing was prospectively performed on 539 children aged between 4 and 17 years with respiratory allergy. the reaction was c ... | 2004 | 15783130 |
| [effects of grazing on architecture and small-scale pattern of grasses on artificial grassland in subtropical zone]. | this study was conducted on a 5-year artificial grassland in subtropical zone of south china. the main types of established artificial grassland there were dactylis glomerata-lolium prenne-trifolium repens and d. glomerata-t. repens pastures. four grazing intensities were designed, i.e., ck (no grazing), g1 (6 adult sheep x hm(-2)), g2 (7.5 adult sheep x hm(-2)) and g3 (10 adult sheep x hm(-2)), and all the grazing plots were rotationally grazed. the architecture and small-scale pattern of grass ... | 2004 | 15825439 |
| myeloperoxidase release after allergen-specific conjunctival challenge. | allergen-specific conjunctival challenge is a fruitful and complete tool in evaluating pathophysiological phenomena of allergic inflammation. after challenge, a significant neutrophil infiltrate occurred in allergic subjects. the primary (azurophilic) granules of neutrophils contain a variety of enzymes that might potentiate inflammation, such as myeloperoxidase (mpo). it is not known whether allergen-specific conjunctival challenge (ascc) is able to elicit mpo release. we also investigated the ... | 2004 | 15584313 |
| monoclonal antibody against a cell wall marker protein for embryogenic potential of dactylis glomerata l. suspension cultures. | we identified and isolated a monoclonal antibody (mab 3g2) raised against extracellular proteins from microcluster cells of orchard grass (dactylis glomerata l.) embryogenic suspension culture. mab 3g2 recognized with high specificity an antigen ionically bound within the primary cell wall and in the culture medium of microcluster cells. two-dimensional polyacrylamide gel analysis and blotting of proteins on pvdf membrane showed that mab 3g2 detected a single polypeptide of apparent molecular ma ... | 2005 | 16025341 |
| orchard grass or cock's foot, dactylis glomerata. | 2005 | 15945550 | |
| immunotherapy with depigmented glutaraldehyde-polymerized extracts: changes in quality of life. | immunotherapy (it) with modified allergens reduces allergic rhinitis (ar) symptoms and medications requirements. improvement of quality of life (qol) is a key point in the treatment of ar. the aim of this study was to provide evidence of changes related to the patient's qol (well-being) induced by a modified (depigmented glutaraldehyde-polymerized) therapeutic vaccine and of its safety. | 2005 | 15898977 |
| aerobic stability of wheat and orchardgrass round-bale silages during winter. | using recently developed technology, balage is often stored in large (1.2 x 1.2 m) round bales that are wrapped in plastic film with an in-line wrapper. the aerobic stability of this fermented forage is important, particularly during winter months when it is fed to livestock or sold as a cash crop. two types of forage, orchardgrass [dactylis glomerata l.; 54.4% dry matter (dm)] and wheat (triticum aestivum l.; 62.4% dm), were packaged in large round bales and wrapped with an in-line wrapper duri ... | 2005 | 15829675 |
| seasonal patterns of growth, dehydrins and water-soluble carbohydrates in genotypes of dactylis glomerata varying in summer dormancy. | summer dormancy in perennial grasses has been studied inadequately, despite its potential to enhance plant survival and persistence in mediterranean areas. the aim of the present work was to characterize summer dormancy and dehydration tolerance in two cultivars of dactylis glomerata (dormant 'kasbah', non-dormant 'oasis') and their hybrid using physiological indicators associated with these traits. | 2005 | 15760915 |
| the major grass pollen group 5 allergen from dactylis glomerata and its c-terminal split product both behave as dimers: implications for allergen standardization. | on sds-page grass pollen group-5 allergens migrate as a doublet with an apparent molecular mass (m(r)) of 25 kda. immunoblot analysis revealed additional group 5 reactivity at double and half this m(r). the aim of this study was to investigate these group 5 molecular entities and to compare their allergenicity and behavior in quantitative immunoassays. | 2005 | 15650308 |
| antigenic activity of nonpollen parts (leaves and stems) of allergenic plants (parietaria judaica and dactylis glomerata). | in patients with respiratory allergy to pollen it is common to correlate the onset, duration and intensity of clinical symptoms with the count of atmospheric allergenic pollen grains. pollen counts, however, may not reflect the total airborne allergen exposure since previous data suggest that pollen allergens may also be carried in microaerosol suspensions. these microdroplets may penetrate deeply into the airways, where pollen grains are too large to penetrate, eventually inducing asthma. the o ... | 2005 | 1952298 |
| a cd14 gene polymorphism is associated with the ige level for dermatophagoides pteronyssinus. | cd14/-159 plays a role in sensitization to dermatophagoides pteronyssinus in japanese patients with allergic rhinitis. | 2005 | 16109674 |
| ruminal in situ disappearance kinetics of dry matter and fiber in growing steers for common crabgrass forages sampled on seven dates in northern arkansas. | southern crabgrass (digitaria ciliaris [retz.] koel.) is often viewed as an undesirable weed, largely because it encroaches upon field and forage crops, gardens, and lawns. however, visual observations of livestock grazing mixed-species pastures suggest that cattle seem to prefer crabgrass to many other summer forages. the objectives of this study were to assess the nutritive value of crabgrass sampled weekly between july 11, and august 22, 2001, and then to determine ruminal in situ disappearan ... | 2005 | 15827259 |
| factors affecting translation at the programmed -1 ribosomal frameshifting site of cocksfoot mottle virus rna in vivo. | the ratio between proteins p27 and replicase of cocksfoot mottle virus (cfmv) is regulated via a -1 programmed ribosomal frameshift (-1 prf). a minimal frameshift signal with a slippery u uua aac heptamer and a downstream stem-loop structure was inserted into a dual reporter vector and directed -1 prf with an efficiency of 14.4 +/- 1.9% in yeast and 2.4 +/- 0.7% in bacteria. p27-encoding cfmv sequence flanking the minimal frameshift signal caused approximately 2-fold increase in the -1 prf effic ... | 2005 | 15843686 |
| [sensitization to castanea sativa pollen and pollinosis in northern extremadura (spain)]. | castanea sativa pollen allergy has generally been considered to be uncommon and clinically insignificant. in our geographical area (plasencia, cáceres, spain) castanea sativa pollen is a major pollen. | 2005 | 15946627 |
| surfactant protein d decreases pollen-induced ige-dependent mast cell degranulation. | mast cells play a key role in allergy and asthma. they reside at the host-environment interface and are among the first cells to make contact with inhaled microorganisms and particulate antigens. pulmonary surfactant proteins a and d (sp-a and sp-d) function in lung host defense by enhancing microbe phagocytosis and mediating other immune cell functions, but little is known about their effects on mast cells. we hypothesized that sp-a and/or sp-d modulate ige-dependent mast cell functions. pollen ... | 2005 | 15980037 |
| [the frequency of pollen allergy at the population of sarajevo region during the 2002 year]. | the frequency of pollen alergy in the sample of population of sarajevo region during the 2002 year has been investigated. the age and gender population has been different. for determination of allergy has been use of standard test carryed out in the respective laboratories of clinical center of university of sarajevo. the testing has been on the pollen of weed plant species and grass (graminejae) pollen. in the mixture of pollen weed plant species have been following plants: plantago lanceolata ... | 2005 | 16018386 |
| limited genetic exchanges between populations of an insect pest living on uncultivated and related cultivated host plants. | habitats in agroecosystems are ephemeral, and are characterized by frequent disturbances forcing pest species to successively colonize various hosts belonging either to the cultivated or to the uncultivated part of the agricultural landscape. the role of wild habitats as reservoirs or refuges for the aphid sitobion avenae that colonize cultivated fields was assessed by investigating the genetic structure of populations collected on both cereal crops (wheat, barley and oat) and uncultivated hosts ... | 2005 | 16024367 |
| surveys of new and rare microfungi in the düsseltal (north rhine-westphalia)--germany. | in the years 2003 and 2004 we have observed an about 70 hectare large area in the düsseltal, the eastern part of the neandertal in north rhine-westphalia. there we collected on trees, bushes and herbs and found about 150 microfungi of which some are new for germany or the entire world. e.g.: pseudocercospora populigena n. ale-agha, u. braun & g.b. feige on populus berolinensis; vialaea insculpta (fr.) sacc. on ilex aquifolium l.; passalora amelopsidis (peck.) u. braun on parthenocissus quinquefo ... | 2005 | 16637187 |
| aerating grassland before manure application reduces runoff nutrient loads in a high rainfall environment. | the effect of mechanically aerating grassland before liquid manure application in the fall on surface runoff and transport of nutrients and solids was studied in a high rainfall area. the two treatments were control and aeration, the latter receiving one pass with an aerator perpendicular to the slope before fall application of liquid manure (dairy in years 1-3 and swine in year 4). treatments were randomly assigned on 3 to 5% sloping land with a silt loam surface soil (aquic dystroxerept) plant ... | 2006 | 16641328 |
| changes in terpene content in milk from pasture-fed cows. | changes of terpene content in milk from cows grazing natural diversified upland pasture were examined in this observational trial. a homogeneous plot divided into 2 subplots was used from may 31 to july 1, 2003 (first growth) and again from october 1 to october 7, 2003 (vegetative regrowth). each subplot was grazed by 6 dairy cows in 2 ways: strip grazing (sg), with new allocations of pasture strips at 2-d intervals, and paddock grazing (pg). the pg subplot was divided into 3 paddocks and the co ... | 2006 | 16702298 |
| p1 protein of cocksfoot mottle virus is indispensable for the systemic spread of the virus. | cocksfoot mottle sobemovirus (cfmv) encodes a non-conserved protein p1 from the 5' orf1 of genomic rna. the functions of cfmv p1 are unknown. in the current study we show that p1-deficient cfmv can replicate both in oat leaves and barley suspension culture cells but can not infect oat plants systemically. however, the absence of p1 reduces the efficiency of virus accumulation considerably. the infectivity of the mutant virus restores as a result of the spontaneous transversion. cfmv p1:egfp show ... | 2006 | 16732485 |
| an attempt to identify recombinants between two sobemoviruses in doubly infected oat plants. | recombination in rna viruses is considered to play a major role as a driving force in virus variability to counterbalance loss in fitness that can be due to the accumulation of detrimental mutations. studies on mixed infections are pertinent for understanding the role of recombination in virus evolution. they also provide important baseline information for studying the biosafety of plants expressing viral sequences. to investigate the possibility of rna recombination occurrence between two sobem ... | 2006 | 16978574 |
| ruminal in situ disappearance kinetics of nitrogen and neutral detergent insoluble nitrogen from common crabgrass forages sampled on seven dates in northern arkansas. | southern crabgrass (digitaria ciliaris [retz.] koel.) is often an undesirable species in field and forage crops, but visual observations suggest that livestock prefer it to many other summer forages. the objectives of this study were to assess the nutritive value of crabgrass sampled weekly between july 11 and august 22, 2001 and then to determine ruminal in situ disappearance kinetics of n and neutral detergent insoluble n (ndin) for these forages. a secondary objective was to compare these kin ... | 2006 | 16478959 |
| bioaccumulation of heavy metals in dactylis glomerata l. growing in a calcareous soil amended with sewage sludge. | the total and dtpa-extractable concentrations of pb, ni, zn, cu, cr and cd were measured in a calcareous soil amended with different doses of sewage sludge under field conditions. the same metals were also measured in the roots and leaves of dactylis glomerata at the end of the first vegetative period after the sludge was added. the root concentrations of all the metals were unrelated to their concentrations in the soil. leaf concentrations of zn and cr correlated with total (zn) and dtpa-extrac ... | 2006 | 15953718 |
| simulation of germination of pioneer species along an experimental drought gradient. | the germination of ten plant species from the iberian peninsula was assessed along a water deficit gradient between -0. 1652 (moist) and -0.4988 mpa (dry) of osmotic potential, created by addition of increasing concentrations of polyethylene glycol (peg 6000) to distilled water in which plants were grown hydroponically. the level and rate of germination of daucus carota and thapsia villosa significantly decreased with decreasing psi. seeds of dactylis glomerata and dittrichia viscosa had positiv ... | 2006 | 17405330 |
| [genetic diversity of dactylis glomerata germplasm resources detected by inter-simple sequence repeats (issrs) molecular markers]. | inter-simple sequence repeat (issr) molecular markers were used to detect the genetic diversity among 50 materials of dactylis glomerata collected from china and other countries. twelve primers produced 101 polymorphic bands, averaged 8.41 bands each primer pair. the average percentage of polymorpgic bands was 86.3.8%, and the range of gs (define) was 0.6116-0.9290, indicating a rich genetic diversity of d. glomerata. based on the cluster and principal component analyses on the genetic character ... | 2006 | 16963418 |
| seed germination in response to chemicals: effect of nitrogen and ph in the media. | seed germination generally presents a peak in the next growing season after a fire. among other factors associated with fire are the increase of soil nitrogen and changes in the ph of the soil. in this study, we addressed the question, whether or not the germination response of eight species is linked with the increase in ph and nitrogenous compounds in the germination media? we assessed the separate and combined effects of nitrogenous compounds and ph on the percentage and rate of germination o ... | 2006 | 16850869 |
| [aflp analysis on genetic diversity of wild dactylis glomerata l. germplasm resources]. | thirty-seven accessions of wild dactylis glomerata l. were used in assessment of genetic diversity by means of amplified fragment length polymorphism (aflp). nine primer pairs were selected from 64 preparations and then used to analyze the plant materials. a total of 400 dna fragments were amplified, among which 336 (84.0%) were polymorphic. genetic distance, from 0.0692-0.4214 among accessions, was calculated by the ntsys-pc software. a dendrogram was constructed using upgma based on genetic di ... | 2006 | 16825173 |
| fractional and structural characterization of hemicelluloses from perennial ryegrass (lolium perenne) and cocksfoot grass (dactylis glomerata). | sequential three-stage treatments with 80% etoh containing 0.2% naoh, 2.5% h2o2-0.2% edta containing 1.5% naoh and 2.5% h2o2-0.2% taed containing 1.0% naoh at 75 degrees c for 3h released 8.0% and 10.4%, 79.1% and 77.0% and 12.9% and 12.5% of the original hemicelluloses from perennial grass and cocksfoot grass, respectively. it was found that the four alkaline peroxide-soluble hemicellulosic fractions contained higher amounts of xylose (33.4-38.2%), uronic acids (9.3-15.3%) and rhamnose (3.0-3.9 ... | 2006 | 16750181 |
| effects of management regime and plant species on the enzyme activity and genetic structure of n-fixing, denitrifying and nitrifying bacterial communities in grassland soils. | management by combined grazing and mowing events is commonly used in grasslands, which influences the activity and composition of soil bacterial communities. whether observed effects are mediated by management-induced disturbances, or indirectly by changes in the identity of major plant species, is still unknown. to address this issue, we quantified substrate-induced respiration (sir), and the nitrification, denitrification and free-living n(2)-fixation enzyme activities below grass tufts of thr ... | 2006 | 16689721 |
| effect of manure application timing, crop, and soil type on nitrate leaching. | timing of manure application affects n leaching. this 3-yr study quantified n losses from liquid manure application on two soils, a muskellunge clay loam and a stafford loamy sand, as affected by cropping system and timing of application. dairy manure was applied at an annual rate of 93 800 l ha(-1) on replicated drained plots under continuous maize (zea mays l.) in early fall, late fall, early spring, and as a split application in early and late spring. variable rates of supplemental sidedress ... | 2006 | 16510712 |
| a comparison of two grass pollen extracts in the treatment of hay fever. | this trial of hyposensitisation compares the efficacy and safety of an alum absorbed 2-grass extract (phleum pratense and dactylis glomerata) with a traditional pyridine extracted alum precipitated 5-grass preparation whose efficacy has been demonstrated previously. 33 patients, (20 alpare, 13 allpyral), were entered into the trial which continued for 3 years; in the second year there were 17 alpare and 11 allpyral patients, and 13 alpare and 10 allpyral in the third year. patients on alpare req ... | 2006 | 3618442 |
| [evolution of microflora during the conservation of forage in microsilos]. | fresh and wilted samples of lolium italicum a. br., dactylis glomerata l., medicago sativa l., have been ensiled in laboratory microsilos for the study of microflora evolution. the principal microbial groups (coliforms, proteolytic bacteria, clostridia, lactic acid bacteria, yeasts) have been detected and enumerated, in anaerobic atmosphere, after 8, 15, 22, 29, 36, 180 days of ensiling. although the number of lactic acid bacteria resulted to the high in all the samples studied, they failed to p ... | 2006 | 7187351 |
| critical evaluation of an indirect enzyme-linked immunosorbent assay for igg antibodies to dactylis glomerata. | experiments are reported for the evaluation of an indirect elisa using peroxidase conjugate for the detection of igg antibodies to dactylis glomerata (dg), a grass pollen antigen frequently responsible for allergic rhinitis and asthma. when optimized, the test proved to be useful in clinical practice for a semiquantitative, cheap and simply-performed determination of igg antibodies to dg. the advantages and disadvantages of this method, compared with those of elaborate antigen-binding assays, ar ... | 2007 | 4081522 |
| uptake, transport, re-translocation and chelation of ni in one tolerant and one susceptible graminaceous species. | from flora of northwest iran, two graminaceous species including secale montanum and dactylis glomerata with different distribution and coverage on ni-rich soils were selected for study of tolerance mechanisms to ni concentrations up to 100 microm in hydroponic culture medium. shoot and root growth of d. glomerata was inhibited up to 42 and 69% in response to 100 microm ni, respectively, while growth reduction of s. montanum was as low as 20% in shoot and no growth inhibition of root was observe ... | 2007 | 19070044 |
| sobemoviruses possess a common cfmv-like genomic organization. | based on structural differences in the orf2 region, the sobemoviruses have been subdivided into southern cowpea mosaic virus (scpmv)-like and cocksfoot mottle virus (cfmv)-like types of genome organization. however, nearly identical amino acid sequences are encoded by these subgroups in different reading frames of orf2, suggesting that insertion or deletion of appropriate nucleotides could restore similar genomic organizations for these viruses. we resequenced the regions of inconsistency for is ... | 2007 | 17115301 |
| the ryegrass mottle virus genome codes for a sobemovirus 3c-like serine protease and rna-dependent rna polymerase translated via -1 ribosomal frameshifting. | in the course of sobemovirus gene cloning the complete genome of ryegrass mottle virus (rgmov) was sequenced. sequence analysis revealed differences including missing and extraneous nucleotides in comparison to the previously published sequence (zhang, toriyama, takanashi, j. gen. plant pathol. 67, 63 (2001)). a gene coding for a typical sobemovirus 3c-like serine protease was identified in orf2a after multiple sequence alignment analysis. the newly identified 57-amino-acid stretch in orf2a show ... | 2007 | 17356908 |
| cocksfoot mottle virus p1 suppresses rna silencing in nicotiana benthamiana and nicotiana tabacum. | the sobemovirus genome consists of positive sense, single-stranded polycistronic rna. the 5'-terminal orf, encoding the protein p1, is its most variable region. sobemoviral p1 has been described as dispensable for replication but indispensable for systemic infection. the p1 of rice yellow mottle virus-nigerian isolate (rymv-n) is the only rna silencing suppressor reported for sobemoviruses until now. using an agrobacterium-mediated transient assay, we demonstrate here that p1 of cocksfoot mottle ... | 2007 | 16971015 |
| effective rumen degradation of dry matter, crude protein and neutral detergent fibre in forage determined by near infrared reflectance spectroscopy. | the objective of the present study was to examine if near infrared reflectance spectroscopy (nirs) could be used to predict degradation parameters and effective degradation from scans of original forage samples. degradability of dry matter (dm), crude protein (cp) and neutral detergent fibre (ndf) of 61 samples of perennial ryegrass (lolium perenne l.) and orchardgrass (dactylis glomerata l.) was tested by using the in situ technique. the grass samples were harvested at three different stages, e ... | 2007 | 17988354 |
| retrospective study of specific immunotherapy - what should be done in the future. | in this retrospective study, data on 241 atopic patients treated with specific cutaneous immunotherapy during the 1985-2006 period at allergy clinic, university department of dermatology and venereology, were reviewed. the following diagnoses were recorded: atopic dermatitis, pure or in combination with allergic rhinitis or allergic bronchitis, or allergic bronchitis and asthma, allergic rhinitis, allergic conjunctivitis, urticaria, and quincke's edema. the aim was to retrospectively analyze cli ... | 2007 | 18093449 |
| phenotypes and functional effects caused by various viral rna silencing suppressors in transgenic nicotiana benthamiana and n. tabacum. | rna silencing suppressor genes derived from six virus genera were transformed into nicotiana benthamiana and n. tabacum plants. these suppressors were p1 of rice yellow mottle virus (rymv), p1 of cocksfoot mottle virus, p19 of tomato bushy stunt virus, p25 of potato virus x, hcpro of potato virus y (strain n), 2b of cucumber mosaic virus (strain kin), and ac2 of african cassava mosaic virus (acmv). hcpro caused the most severe phenotypes in both nicotiana spp. ac2 also produced severe effects in ... | 2008 | 18184062 |
| specific immunotherapy for common grass pollen allergies: pertinence of a five grass pollen vaccine. | patients throughout europe are concomitantly exposed to multiple pollens from distinct pooideae species. given the overlap in pollination calendars and similar grain morphology, it is not possible to identify which grass species are present in the environment from pollen counts. furthermore, neither serum ige reactivity nor skin prick testing allow the identification of which grass species are involved in patient sensitisation. due to their high level of amino acid sequence homology (e.g., >90% ... | 2008 | 18362477 |
| forage systems for cow-calf production in the appalachian region. | small cow-calf operations are common in the appalachian region. tall fescue [lolium arundinaceum (schreb.) s. j. darbyshire] is the dominant forage in these systems for direct grazing as well as for stockpiling. the present study was conducted from 2001 to 2005. a total of 108 angus and angus crossbred cows were allotted randomly to 6 forage systems and then to 3 replicates within each system. in brief, system 1 had a stocking rate of 0.91 ha/cow in a middleburg 3-paddock (a, b, and c) system. s ... | 2008 | 18407993 |
| biological and molecular characterization of a putative new sobemovirus infecting imperata cylindrica and maize in africa. | a new virus was isolated from both the grass imperata cylindrica and maize plants that had yellow mottle symptoms in burkina faso, west africa. the virus has isometric particles ca. 32 nm in diameter. the experimental host range was restricted to rottboellia exaltata. virions were isolated from leaves of systemically infected maize plants. koch's postulates were completed by mechanically inoculating uninfected imperata or maize with either purified virus or sap from infected imperata plants. vir ... | 2008 | 18777157 |
| [physiological bases of herbages shade-tolerance in eucalyptus grandis and herbage inter-cultivated system]. | the study on the physiological bases of the shade-tolerance of dactylis glomerata, lolium perenne, and hemarthria compressa inter-cultivated in eucalyptus grandis forests with different canopy densities showed that with increasing canopy density, the light saturation point (lsp), light compensation point (lcp), chlorophyll a/b (chla/chlb), and the contents of proline (pro), soluble sugar (ss) and soluble protein (sp) of the three herbages decreased gradually, but the apparent quantum yield (aqy) ... | 2008 | 19102302 |
| antigenic similarity among group 1 allergens from grasses and quantitation elisa using monoclonal antibodies to phl p 1. | group 1 allergens elicit a specific ige response in about 90% of grass pollen-allergic patients. the aim of this work was to study the antigenic similarity among group 1 allergens from different grasses and to develop a monoclonal antibody (mab)-based quantitation elisa. | 2008 | 18004069 |
| effect of intake level and alfalfa substitution for grass hay on ruminal kinetics of fiber digestion and particle passage in beef cattle. | two experiments were conducted to evaluate digestion kinetics of alfalfa (medicago sativa l.) substitution for grass hay in beef cattle. in exp. 1, forage combinations evaluated in situ consisted of 0% alfalfa-100% big bluestem (andropogon gerardi vitman), 25% alfalfa-75% big bluestem, 50% alfalfa-50% big bluestem, and 100% alfalfa-0% big bluestem. nonlinear regression was used to determine the immediately soluble fraction a, the potentially degradable fraction b, the undegraded fraction c, and ... | 2008 | 17940159 |
| extraction, structural characterisation and evaluation of hydroxycinnamate esters of orchard grass (dactylis glomerata) as substrates for polyphenol oxidase. | polyphenol oxidase (ppo) activity has been reported in orchard grass (dactylis glomerata); however, to date, no endogenous substrates have been identified. in the present study, we report the isolation and structural elucidation of ppo substrates in this species. the free phenol fraction was extracted, separated by reverse-phase chromatography and six potential substrates, including two hydroxycinnamate esters, were identified by uv spectrometry, electrospray ionisation-tandem mass spectrometry ... | 2008 | 18929376 |
| double-blind, placebo-controlled evaluation of grass pollen specific immunotherapy with oral drops administered sublingually or supralingually. | forty-one patients suffering from grass pollen allergy underwent specific immunotherapy with standardized allergen extract consisting of six grass pollens (h-al per os) administered either sublingually or supralingually for one year. in order to investigate clinical and immunological changes induced by the administration of allergens via the oral mucosa, the double-blind, placebo-controlled, randomized design of the trial with 30 other patients enrolled in placebo groups was applied. specific im ... | 2008 | 18585908 |
| cluster immunotherapy with a glutaraldehyde-modified mixture of grasses results in an improvement in specific nasal provocation tests in less than 2.5 months of treatment. | cluster immunotherapy is becoming increasingly used. it allows for a rapid build up phase and the administration of higher doses of allergen in a shorter period of time. | 2008 | 18445082 |
| evidence for gc preference by monocot dicer-like proteins. | dicot dicer-like (dcl) enzymes operate preferably on gc rich regions when producing small interfering (si)rna and micro (mi)rna. this gc bias, however, is not generic in monocot mirna productions. from wild dactylis glomerata naturally infected by cocksfoot streak potyvirus (csv), csv-sirnas had a greater gc% than the virus genome, indicating that gc rich regions were also preferred by the grass dcls. this supports the notion that gc preference is an ancient feature for plant dcls, and suggests ... | 2008 | 18243131 |
| unraveling the effects of management regime and plant species on soil organic carbon and microbial phospholipid fatty acid profiles in grassland soils. | in a field experiment we have examined the effect of long-term grassland management regimes (viz., intensive versus extensive) and dominant plant species (viz., arrhenatherum elatius, holcus lanatus and dactylis glomerata) on soil organic carbon (soc) build up, soil microbial communities using biomarker phospholipid fatty acids (plfa), and the relationship between soc and plfas of major groups of microorganisms (viz., bacteria, fungi, and actinomycetes). the results have revealed that changes in ... | 2008 | 17826981 |
| [detection and characteristics of defective interfering rna associated with cocksfoot mottle sobemovirus]. | 2008 | 10779963 | |
| ultrastructural analyses of somatic embryo initiation, development and polarity establishment from mesophyll cells of dactylis glomerata. | somatic embryos initiate and develop directly from single mesophyll cells in in vitro-cultured leaf segments of orchardgrass (dactylis glomerata l.). embryogenic cells establish themselves in the predivision stage by formation of thicker cell walls and dense cytoplasm. electron microscopy observations for embryos ranging from the pre-cell-division stage to 20-cell proembryos confirm previous light microscopy studies showing a single cell origin. they also confirm that the first division is predo ... | 2008 | 11681324 |
| [sobemovirus genome from dactylis glomerata has a luteovirus-like organization of the replicase and protease gene modules]. | 2008 | 8754004 | |
| [specific skin reactions induced by individual pollen preparations in hypersensitivity persons]. | skin tests are the most useful single modality for demonstrating an ige-mediated mechanism underlying clinical symptoms. skin reactivity to an allergen depends on person's exposure and genetic factors influencing the ige response. however, the reproducibility of allergy skin tests has been shown to be variable depending, among other things, on the allergen extract employed and the kind of technique used. these variations have led to controversy regarding clinical relevancy of allergy skin testin ... | 2009 | 9863408 |
| proof of concept pilot study: prevalence of grass virus infection and the potential for effects on the allergenic potency of pollen. | wild plants harbour a variety of viruses and these have the potential to alter the composition of pollen. the potential consequences of virus infection of grasses on pollen-induced allergic disease are not known. | 2009 | 20102577 |
| characterization of allergenic epitopes of ory s1 protein from oryza sativa and its homologs. | vaccination is the most effective technique suggested now days for allergy treatment. recombinant-based approaches are mostly focused on genetic modification of allergens to produce molecules with reduced allergenic activity and conserved antigenicity. the molecules developed for vaccination in allergy possess significantly reduced allergenicity in terms of ige binding, and therefore will not lead to anaphylactic reactions upon injection. this approach is probably feasible with every peptide all ... | 2009 | 20011147 |
| comparative sequence analysis and serological and infectivity studies indicate that cocksfoot mild mosaic virus is a member of the genus panicovirus. | the complete nucleotide sequence of the phleum isolate of cocksfoot mild mosaic virus (cmmv-p) and the coat protein sequence of the cocksfoot isolate (cmmv-1) were determined. comparative sequence analysis revealed a close relationship with panicum mosaic virus (pmv; genus panicovirus), and together with serological studies, the work supports the classification of cmmv in the family tombusviridae, genus panicovirus rather than, as is currently proposed, the genus sobemovirus. a full-length cdna ... | 2009 | 19693646 |
| [genetic variation and relationship in orchardgrass (dactylis glomerata l.) germplasm detected by ssr markers]. | genetic variation and relationship of 53 accessions of d. glomerata collected from 5 continents were analyzed using simple sequence repeat (ssr) molecular markers with 15 ssr primer pairs. the following results were obtained. (1) a total of 127 alleles were detected at 15 loci. the number of alleles per locus ranged from 5 to 12, with an average of 8.5. the rate of polymorphic sites (p) was 95.21%; the polymorphic information content (pic) ranged from 0.30(a04c24)to 0.44(a01f24) with an average ... | 2009 | 19586867 |
| biodiversity effects on yield and unsown species invasion in a temperate forage ecosystem. | current agricultural practices are based on growing monocultures or binary mixtures over large areas, with a resultant impoverishing effect on biodiversity at several trophic levels. the effects of increasing the biodiversity of a sward mixture on dry matter yield and unsown species invasion were studied. | 2009 | 19168861 |
| cross-species amplification tests and diversity analysis using 56 pcr markers in dactylis glomerata and lolium perenne. | we report results of cross-species amplification in dactylis glomerata and lolium perenne of 12 simple sequence repeats (ssrs) isolated from lolium multiflorum×festuca glaucescens, 42 ssrs from festuca arundinacea and two sequence tagged sites from oryza sativa. we compared the transferability and diversity between d. glomerata and l. perenne, which are important forage crops. while nei's gene diversity values were equivalent in both species (from 0.14 to 0.92), the mean number of allele per loc ... | 2009 | 21564590 |
| generation of recombinant antibodies against orchardgrass acidic nsltp-like proteins. | embryogenic and non-embryogenic suspension cultures of orchardgrass (dactylis glomerata l.) secreted into the culture medium a set of proteins, among which low molecular mass (11/12 kda) proteins were found. however, only the 11/12 kda proteins from the embryogenic suspension cultures reacted specifically with an antiserum raised against the carrot ep2 non-specific lipid transfer protein (nsltp). two-dimensional (2-d) electrophoretic analysis revealed that the extracellular nsltp-like proteins f ... | 2009 | 18669026 |
| biogas production from boreal herbaceous grasses--specific methane yield and methane yield per hectare. | the objective of this study was to determine the specific methane yields of four grass species (cocksfoot, tall fescue, reed canary grass and timothy) cultivated under boreal conditions as well as how harvesting time and year of cultivation affects the specific methane yields per ha. the specific methane yields of all grasses and all harvests varied from 253 to 394 nl ch4/kg volatile solids (vs) added. the average specific methane yield of the 1st harvest of all grasses was higher than the 2nd h ... | 2009 | 19261471 |
| a leachate a day keeps the seedlings away: mowing and the inhibitory effects of festuca paniculata in subalpine grasslands. | is the release of allelochemicals by the dominant tussock grass festuca paniculata responsible for its dominance by inhibiting growth of neighbour grasses in subalpine grasslands? as such a community is also structured by mowing practices, what could be the impact of mowing on allelopathy? | 2009 | 19324898 |
| responses of plasma acetate metabolism to hop (humulus lupulus l.) in sheep. | an isotope dilution method using [1-(13)c]sodium (na) acetate was conducted to determine the effect of feeding hop (humulus lupulus l.) residues on plasma acetate metabolism in six adult crossbred sheep. the sheep were fed 63 g/kg bw(0.75)/d of either mixed hay (mh-diet) of orchardgrass (dactylis glomerata l.) and reed canarygrass (phalaris arundinacea l.) at a 60:40 ratio or mh-diet and hop-residues (hop-diet) at 85:15 ratio with a crossover design for each of 3 week period. the isotope dilutio ... | 2009 | 19365576 |
| water deficit and induction of summer dormancy in perennial mediterranean grasses. | summer dormancy is a trait conferring superior drought survival in mediterranean perennial grasses. as the respective roles of environmental factors and water deficit on induction of summer dormancy are unclear, the effect of intense drought were tested under contrasting day lengths in a range of forage and native grasses. | 2009 | 19369219 |
| effects of rumen fill on short-term ingestive behavior and circulating concentrations of ghrelin, insulin, and glucose of dairy cows foraging vegetative micro-swards. | the objective of this study was to investigate changes in foraging behavior, hunger-related hormones, and metabolites of dairy cows in response to short-term variations in rumen fill (rf). the effect of rf on intake rate, jaw movements, bite rate and dimensions, and concentrations of plasma ghrelin, and serum insulin and glucose were measured in 4 rumen-cannulated lactating dairy cows (612 +/- 68 kg, empty live weight; 237 +/- 29 d in milk) foraging micro-swards of vegetative orchardgrass (dacty ... | 2009 | 19389967 |
| effects of bale moisture and bale diameter on spontaneous heating, dry matter recovery, in vitro true digestibility, and in situ disappearance kinetics of alfalfa-orchardgrass hays. | alfalfa (medicago sativa l.)-orchardgrass (dactylis glomerata l.) hay was made in 96 large-round bales over 3 harvests during 2006 and 2007 to assess the effects of spontaneous heating on dry matter (dm) recovery, in vitro true digestibility (ivtd), and in situ disappearance kinetics of dm. throughout these harvests, bales were made at preset diameters of 0.9, 1.2, or 1.5 m and at moisture concentrations ranging from 9.3 to 46.6%. internal bale temperatures were monitored daily during an outdoor ... | 2009 | 19448019 |
| effects of spontaneous heating on fiber composition, fiber digestibility, and in situ disappearance kinetics of neutral detergent fiber for alfalfa-orchardgrass hays. | during 2006 and 2007, forages from 3 individual hay harvests were utilized to assess the effects of spontaneous heating on concentrations of fiber components, 48-h neutral detergent fiber (ndf) digestibility (ndfd), and in situ disappearance kinetics of ndf for large-round bales of mixed alfalfa (medicago sativa l.) and orchardgrass (dactylis glomerata l.). over the 3 harvests, 96 large-round bales were made at preset bale diameters of 0.9, 1.2, or 1.5 m, and at moisture concentrations ranging f ... | 2009 | 19448020 |
| safety and tolerability of seasonal ultra-rush, high-dose sublingual-swallow immunotherapy in allergic rhinitis to grass and tree pollens: an observational study in 193 children and adolescents. | we conducted a large observational study in 193 children and adolescents with allergic rhinitis due to grass or tree pollens to evaluate the safety and tolerability of an ultrarush high-dose sublingual immunotherapy (slit) regimen reaching a maintenance dose of 300 index of reactivity within 90 minutes. | 2009 | 19476016 |
| fatty acid metabolism and deposition in subcutaneous adipose tissue of pasture- and feedlot-finished cattle. | an experiment was conducted to evaluate the effects of pasture finishing versus feedlot finishing, over time, on fatty acid metabolism in angus crossbred steers (n = 24). ruminal fluid, serum, and adipose tissue biopsies were obtained on d 0, 28, 84, and 140. pasture forages and diet ingredient samples were obtained at 14-d intervals to determine nutritive value and fatty acid composition. the feedlot diet consisted of corn silage, cracked corn grain, soybean meal, and a vitamin and mineral supp ... | 2009 | 19617512 |
| effect of supplementation of grazing dairy ewes with a cereal concentrate on animal performance and milk fatty acid profile. | this work was conducted to investigate the effect of supplementing grazing ewes on pasture with a cereal concentrate on the milk fatty acid (fa) profile. ninety assaf ewes in mid lactation were distributed in 9 lots of 10 animals each and allocated to 3 feeding regimens: 1) pasture--ewes were only allowed to graze pasture (an irrigated sward of lolium perenne, trifolium pratense, and dactylis glomerata); 2) ps--grazing ewes were supplemented with oat grain (700 g/animal and day); and 3) tmr--ewe ... | 2009 | 19620680 |
| stem-loop structure of cocksfoot mottle virus rna is indispensable for programmed -1 ribosomal frameshifting. | the -1 programmed ribosomal frameshifting (-1 prf) mechanism utilized by many viruses is dependent on a heptanucleotide slippery sequence and a downstream secondary structure element. in the current study, the rna structure downstream from the slippery site of cocksfoot mottle sobemovirus (cfmv) was proven to be a 12bp stem-loop with a single bulge and a tetranucleotide loop. several deletion and insertion mutants with altered stem-loop structures were tested in wheat germ extract (wge) for fram ... | 2009 | 19748532 |
| rhizosphere microbial densities and trace metal tolerance of the nickel hyperaccumulator alyssum serpyllifolium subsp. lusitanicum. | in this study we determine culturable microbial densities (total heterotrophs, ammonifiers, amylolytics and cellulolytics) and bacterial resistance to co, cr, and ni in bulk and rhizosphere soils of three populations of the ni-hyperaccumulator alyssum serpyllifolium subsp. lusitanicum and the excluder dactylis glomerata from ultramafic sites (two populations in northeast (ne) portugal (samil (s), morais (m)) and one population in northwest (nw) spain (melide (l)). the relationship between bioava ... | 2009 | 19810353 |
| [examination about positive ratio of pollen antigens by scratch test]. | japanese cedars and grass are famous for causing pollen allergy with antigen. but recently, many other antigens causing pollen allergy are coming to the front. we investigated positive ratios of pollen antigens of the consultation patients. | 2009 | 20220303 |
| ultraviolet-absorbing compounds in milk are related to forage polyphenols. | the aim of this work was to characterize uv-absorbing compounds (uac) in milk in relation to diet. in winter, 4 groups of cows each received a different diet: concentrate rich containing 35% cocksfoot hay (cch), maize silage (ms), rye grass silage (rs), or rye grass hay (rh). in summer, 2 additional diets were given: mountain grassland hay (gh) and mountain grassland pasture (gp). polyphenols were analyzed by hplc and folin reaction on forages and uac were extracted from milks and analyzed by hp ... | 2010 | 20630201 |
| which allergen extract for grass pollen immunotherapy? an in vitro study. | grass pollen is a major cause of allergy throughout the world. the only treatment targeting the causes and not only the symptoms of allergy is specific immunotherapy (it). a number of controlled trials demonstrated the efficacy of it in grass pollen allergic subjects, most using extracts of multiple grasses but some using extracts of a single grass. the optimal grass extract for it has not yet been established. | 2010 | 20653430 |
| bridging allergologic and botanical knowledge in seasonal allergy: a role for phenology. | grass pollen is a worldwide cause of respiratory allergy. identifying the causative species is essential, for example for choosing the appropriate immunotherapy, because not all grass allergens are totally cross-reacting, and the pollen calendars provide only a gross estimate. phenologic analyses allow identification of the pollen release for each individual grass. | 2010 | 20800789 |
| protein-rna linkage and post-translational modifications of two sobemovirus vpgs. | sobemoviruses possess a viral genome-linked protein (vpg) attached to the 5' end of viral rna. vpg is processed from the viral polyprotein. in the current study, cocksfoot mottle virus (cfmv) and rice yellow mottle virus (rymv) vpgs were purified from virions and analysed by mass spectrometry. the cleavage sites in the polyprotein and thereof the termini of vpg were experimentally proven. the lengths of the mature vpgs were determined to be 78 and 79 aa residues, respectively. the amino acid res ... | 2010 | 21068217 |
| nucleotide bias of dcl and ago in plant anti-virus gene silencing. | plant dicer-like (dcl) and argonaute (ago) are the key enzymes involved in anti-virus post-transcriptional gene silencing (av-ptgs). here we show that av-ptgs exhibited nucleotide preference by calculating a relative av-ptgs efficiency on processing viral rna substrates. in comparison with genome sequences of dicot-infecting turnip mosaic virus (tumv) and monocot-infecting cocksfoot streak virus (csv), viral-derived small interfering rnas (vsirnas) displayed positive correlations between av-ptgs ... | 2010 | 21203927 |
| cocksfoot mottle sobemovirus coat protein contains two nuclear localization signals. | cocksfoot mottle virus (cfmv) coat protein (cp) localization was studied in plant and mammalian cells. fusion of the full-length cp with enhanced green fluorescent protein (egfp) localized to the cell nucleus whereas similar constructs lacking the first 33 n-terminal amino acids of cp localized to the cytoplasm. cp and egfp fusions containing mutations in the arginine-rich motif of cp localized to the cytoplasm and to the nucleus in plant cells indicating the involvement of the motif in nuclear ... | 2010 | 20155311 |
| effects of spontaneous heating on forage protein fractions and in situ disappearance kinetics of crude protein for alfalfa-orchardgrass hays packaged in large round bales. | during 2006 and 2007, forages from 3 individual hay harvests were used to assess the effects of spontaneous heating on concentrations of crude protein (cp), neutral detergent insoluble cp (ndicp), acid detergent insoluble cp (adicp), and in situ disappearance kinetics of cp and ndicp for large round bales of mixed alfalfa (medicago sativa l.) and orchardgrass (dactylis glomerata l.). over the 3 harvests, 96 large round bales were made at preset bale diameters of 0.9, 1.2, or 1.5m and at moisture ... | 2010 | 20172236 |