Publications

the effects of intake of lucerne (medicago sativa l.) and orchard grass (dactylis glomerata l.) hay on the motility of the forestomach and digesta flow at the abomaso-duodenal junction of the sheep.	1. the relations between food intake, reticulo-ruminal motility and abomasal digesta outflow were investigated in ewes receiving lucerne (medicago sativa) v. orchard grass (dactylis glomerata) hay. abomasal digesta outflow was recorded continuously by means of an electromagnetic flowmeter probe inserted into a duodenal t-shape cannula and the motility using strain-gauge force transducers. volumes and turnover rates of both rumen and abomasal liquid phases were measured by credta dilution. 2. the ...	1989	2758019
relationship between skin-prick tests, the multiple allergosorbent test and symptoms of allergic disease.	in 47 atopic subjects, skin-prick testing to 10 common allergens was performed, and specific ige to the same allergens was assessed by the multi-allergosorbent chemiluminescent assay (mast-cla). overall agreement between the tests was 66.4% for conventionally positive skin tests (weal diameter greater than or equal to 3 mm), rising to 78.5% when a positive skin test was defined as having a weal diameter greater than or equal to 5 mm. agreement between the tests was statistically significant for ...	1989	2649214
[skin reactivity to grass pollen and the mite dermatophagoides pteronyssinus in the prick-test using 2 types of allergen preparations].	the reliability of prick testing with allergen preparations produced in yugoslavia was assessed by comparison to the internationally accepted ones (pharmacia diagnostica) of 100,000 bu activity. the following allergens were used: the mite dermatophagoides pteronyssinus in 107 subjects, and grass pollens: dactylis glomerata, poa pratensis, phleum pratense and secale cereale in 39 subjects. the clinical significance of the response was assessed in relation to the skin reaction to the negative cont ...	1989	2638564
study of the epitope structure of purified dac g i and lol p i, the major allergens of dactylis glomerata and lolium perenne pollens, using monoclonal antibodies.	the use of mab allowed us to further analyze the cross-reactivity between purified dac g i and lol p i, the major allergens of dactylis glomerata (cocksfoot) and lolium perenne (rye grass), respectively. it was first shown, using ief, followed by immunoprinting, that serum ige antibodies from most grass-sensitive patients recognize both dac g i and lol p i. second, three different anti-lol p i mab, 290a-167, 348a-6, and 539a-6, and one anti-dac g i mab, p3b2 were all shown to react with dac g i ...	1988	2460536
thermoinactivation of human ige: antigenic and functional modifications.	the thermoinactivation kinetics of ige were studied in experimental models revealing the antigenic properties and the basophil-sensitizing capacity of these immunoglobulins. a pool of human sera containing anti-dactylis glomerata (dg) ige was heated from 5 min up to 4 hr at 56 degrees. the ige antigenicity was tested by two polyclonal 125i-labelled anti-ige antibodies; one anti-ige was specific of the whole fc epsilon region, while the other had a specificity restricted to the d epsilon 2 domain ...	1986	2420711
purification and characterization of a major allergen from dactylis glomerata pollen: the ag dg1.	we have isolated an allergen (ag dg1) from dactylis glomerata pollen which is recognized by the serum of 95% of human patients sensitive to d. glomerata pollen, as has been shown by the nitrocellulose immunoprint technique. after two successive purifications by preparative isoelectric focusing (ief), dg1 was characterized as a single band in analytical agarose ief with a pi of 5.9 and was found to display 3 bands by sodium dodecyl sulfate polyacrylamide gel with the respective molecular weights: ...	1985	2414230
identification of orchard grass (dactylis glomerata) pollen allergens following electrophoretic transfer to nitrocellulose.	orchard grass (cocksfoot) pollen extracts, fractionated by polyacrylamide gradient electrophoresis or sds gel electrophoresis were electroblotted onto nitrocellulose membranes and probed with sera from orchard grass pollen-allergic patients and 125i-anti-human ige. the ige-binding components of the pollen were detected by autoradiography. elution studies showed that allergens could be extracted immediately and continuously over a 3-hour period. two fractions of mws 28,000 and 30,000 could be det ...	1985	2411668
production of a monoclonal antibody against a major allergen of dactylis glomerata pollen (dg1).	a mouse monoclonal antibody was produced against the most frequent allergen ag dg1ief, purified from the dactylis glomerata grass pollen by isoelectric focusing. using the immunoprint technique, this monoclonal antibody detected ag dg1ief in d. glomerata pollen and some cross-reacting molecules in four other grass pollens. this hybridoma was grown in ascitic fluid. an immunosorbent was made from it and, by affinity chromatography, was used to purify monoclonal ag dg1m from the pollen extract. ag ...	1985	2408551
immunotherapy with alginate-conjugated two grass pollen extract in patients with allergic rhinoconjunctivitis.	the aim of the present study was to evaluate the clinical and immunological effects of specific immunotherapy with alginate-conjugated two grass pollen extract in patients with rhinoconjunctivitis. a total of 52 patients were admitted to the study. before and after 3 years of immunotherapy quantitative skin test (qst) to dactylis glomerata (dg) and to phleum pratense (php), variation of grass specific ige and igg4 and nasal challenge with the aqueous extract of the two grasses were compared. eac ...	2015	2382596
monoclonal antibodies to proteins from cocksfoot grass (dactylis glomerata) pollen: isolation and n-terminal sequence of a major allergen.	monoclonal antibodies (mabs) raised to an aqueous extract of cocksfoot grass (dactylis glomerata) pollen have been characterised. mab 1b9 was demonstrated by sds-page and western blotting to recognise a major allergen of an approximate molecular weight of 28 kd in this extract, termed dg3, and a component with a molecular weight of between 35 and 40 kd in an extract of secale cereale (cultivated rye) pollen. the 28 kd component of cocksfoot grass pollen isolated by affinity chromatography using ...	1990	2210876
characterization of the major allergen of cynodon dactylon (bermuda grass) pollen, cyn d i.	an allergen from cynodon dactylon (bermuda grass) pollen, cyn d i, has been purified by a combination of concanavalin a-sepharose affinity chromatography, and carboxymethyl-sepharose chromatography. the allergen constitutes the major allergenic component of the pollen extract as observed by immunoelectrophoretic techniques, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, an ige-inhibition experiment, and skin testing. in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, cyn d ...	1991	1955635
antigenic activity of nonpollen parts (leaves and stems) of allergenic plants (parietaria judaica and dactylis glomerata).	in patients with respiratory allergy to pollen it is common to correlate the onset, duration and intensity of clinical symptoms with the count of atmospheric allergenic pollen grains. pollen counts, however, may not reflect the total airborne allergen exposure since previous data suggest that pollen allergens may also be carried in microaerosol suspensions. these microdroplets may penetrate deeply into the airways, where pollen grains are too large to penetrate, eventually inducing asthma. the o ...	2005	1952298
rast in diagnosis and therapy of allergic rhinitis.	skin tests, rast, nasal provocation tests, and other nasal allergy examinations were performed on 463 patients exhibiting symptoms of allergic rhinitis who were examined at the department of otolaryngology of osaka city university medical school, and comparisons were made between the rast scores and the results of the other examinations. a study was also made of the change in grade of the allergic rhinitis and the change of the rast score resulting from one year of immunotherapy. the rates of ag ...	1991	1842868
cocksfoot grass pollen allergens and genetic of immune response.	the genetic basis of allergic response to grass pollen allergens of 23 portuguese families have been studied. the two or three generations families formed a total of 128 individuals including at least one parent and one child sensitive to dactylis glomerata grass pollen. hla class ii genes were studied by pcr amplification and rflp analysis. our population study has revealed a higher frequency of allele 2 of dob in the allergic population than in non allergic individuals. 60% of cocksfoot sensit ...	1991	1811648
n-terminal amino acid sequence homologies of group v grass pollen allergens.	in an earlier study, we presented data regarding the immunoaffinity purification and n-terminal sequencing of a major pollen allergen from orchard/cocks-foot grass (dactylis glomerata), now identified as the group v allergen dac g v. in this paper, we have extended our investigations to include group v allergens from other grass species. our data confirm the presence of group v-restricted characteristic n-terminal amino acid sequences containing a high alanine and hydroxyproline (p') rather than ...	1992	1643443
group v allergens in grass pollens: iv. similarities in amino acid compositions and nh2-terminal sequences of the group v allergens from lolium perenne, poa pratensis and dactylis glomerata.	monoclonal antibodies (ppv4) raised against phleum pratense group v allergen were used for immuno-affinity chromatography of cross-reacting group v allergens from related grass species. fractions enriched in group v allergen were obtained from lolium perenne, poa pratense and dactylis glomerata extracts. the major components in these fractions were found in the mwr range 25-28 kd. ige binding to these components was shown using a pool of grass allergic sera, by sds-page immunoblotting. these fra ...	1992	1611548
forage systems for beef production from conception to slaughter: i. cow-calf production.	six year-round, all-forage, three-paddock systems for beef cow-calf production were used to produce five calf crops during a 6-yr period. forages grazed by cows during spring, summer, and early fall consisted of one paddock of 1) tall fescue (festuca arundinacea schreb.)-ladino clover (trifolium repens l.) or 2) kentucky blue-grass (poa pratensis l.)-white clover (trifolium repens l.). each of these forage mixtures was combined in a factorial arrangement with two paddocks of either 1) fescue-red ...	1992	1548221
recombinant pollen allergens from dactylis glomerata: preliminary evidence that human ige cross-reactivity between dac g ii and lol p i/ii is increased following grass pollen immunotherapy.	we previously described the isolation of three identical complementary dna (cdna) clones, constructed from orchard/cocksfoot grass (dactylis glomerata) anther messenger rna (mrna), expressing a 140,000 mw beta-galactosidase fusion protein recognized by ige antibodies in atopic sera. partial nucleotide sequencing and inferred amino acid sequence showed greater than 90% homology with the group ii allergen from lolium perenne (lol ii) indicating they encode the group ii equivalent, dac g ii. wester ...	1992	1526648
[sensitivity and specificity of the determination of ige using chemiluminescence (cla allergy test)].	we studied 80 patients. all patients underwent a thorough clinical evaluation, including a comprehensive study and physical examination of the ear, nose, throat and chest. each patient underwent prick testing and a nasal provocation test. in addition, blood was drawn for the allergen-specific ige test (rast) and total serum ige levels. we selected 11 allergens for this study: grass (dactylis glomerata-orchard grass, phelum pratense-timothy grass, lolium-raygrass), weeds (chenipodium, parietaria) ...	1992	1509983
[conditions required for chemical binding of dactylis glomerata grass pollen to inert stable carriers].	the results of the direct rast (radio-allergo-sorbent-test) procedure used to standardise own-prepared pollen extract of the dactylis glomerata grass are presented. based on the performed standardisation, a system was set up for binding allergens to the stable inert carriers. the results of examination of the quality of d. glomerata allergen, bound covalently to paper disc, are presented also. evaluation was made in inep laboratories by comparing the d. glomerata allergen-bound discs and the cor ...	2015	1481465
variability of crossreactivity of ige antibodies to group i and v allergens in eight grass pollen species.	crossreactivity to dactylis glomerata, festuca rubra, phleum pratense, anthoxanthum odoratum, secale cereale, zea mays, and phragmites communis of ige antibodies against lol p i or lol p v was investigated by means of rast-inhibition. within a group of sera the degree of crossreactivity was demonstrated to be highly variable. individual sera were not always equally crossreactive to all pollen species. a high degree of crossreactivity for group i allergens did not necessarily implicate the same f ...	1992	1382820
influence of hay making on in situ nitrogen degradability of forages in cows.	the influence of hay making on in situ n degradability of forages was assessed by comparing the degradability of nine hays with that of standing forages at the time of harvest. three ryegrass, two cocksfoot, and three alfalfa forages were used. the n degradability of hays was related closely to that of the corresponding standing forages (r = .91) and was, on average, lower (-2.5 points). this decrease in degradability after hay making was not constant; it increased when the degradability of the ...	1992	1314856
forage systems for beef production from conception to slaughter: ii. stocker systems.	fall weaned angus calves grazed stockpiled 1) tall fescue (festuca arundinacea schreb.), 2) tall fescue-red clover (trifolium pratense l.), or 3) tall fescue-alfalfa (medicago sativa l.) or were barn-fed, 4) tall fescue hay, 5) orchardgrass (dactylis glomerata l.)-alfalfa hay, or 6) tall fescue silage from late october to early april during each of 5 yr. infection of the fescue with acremonium coenophialum ranged from 0 to 55%. there were two replications each of steers and heifers for each fora ...	1992	1312526
cutaneous reactions to pollen extracts in patients with hay fever. special references to grass pollen recommended for treatment in fixed combination.	skin reactivity to 8 common grass pollens was investigated in patients with hay fever using commercial, unfractionated extracts in serial dilution technique. the highest degree of sensitivity was observed for cocksfoot, meadow fescue, meadow foxtail, rye grass, timothy and yorkshire fog. five of those are included in the allpyral 5-grass mix. the cutaneous responses to rye grass pollen overlap with those to timothy in a high degree.	1975	1173992
examination of cocksfoot pollen (dactylis glomerata) extracts by skin prick test in man, rast-based allergen assay and protein content.	three commercial preparations of cocksfoot pollen (dactylis glomerata) from different manufacturers were compared by skin prick tests in man, allergen activity as measured by the rast test and protein nitrogen unit (pnu) and total nitrogen (n) content. the skin prick tests were made on the forearm with the undiluted preparations and also with solutions of 1/5 and 1/25 dilutions. three groups each of 9 subjects were tested and the sites for the 9 tests were determined by 3 different latin squares ...	1975	1149937
rast, skin and nasal tests and the history in grass pollen allergy.	a comparison of the rast (phadebas), skin prick test, nasal test and a history of hay fever was made in 141 subjects, eighty-seven being prick test positive, with three concentrations of extracts of sweet vernal (anthoxanthum odoratum), cocksfoot (dactylis glomerata), meadow fescue (festuca elatior), rye (lolium perenne), timothy (phleum pratense), meadow (poa pratensis). in adequate concentration any of the species extracts would have sufficed for skin tests, with cocksfoot pollen the most pote ...	1975	1053442
[obtaining mutations with cytoplasmic male sterility (cms) in dactylis glomerata l. under the influence of chemical mutagens].	when treating seeds of orchard grass with definite concentrations of nitrosoethylurea (neu), dimethyl sulphate (dms) and ethylene imine (ei) in m1 and m2 the plants with cms as well as sterility fixators to them are obtained. proportionality of mutagenic action and stimulation effect of these substances are found. the greatest amount of sterility fixators is found in m1 among plants the seeds of which were treated with neu and ei; in m2 the fixators are found among plants obtained after treating ...	2013	1030857
demonstration of reaginic antibodies on human basophils by immune adherence to allergen-coated sepharose beads.	human basophils purified by gradient density centrifugation and differential glass bead adherence were interacted with grass pollen allergen in particulate form prepared by covalent coupling of dactylis glomerata protein concentrate to sepharose beads. basophils from allergic subjects were found to interact specifically with the allergen-coated bead surface in a manner so highly characteristic that false negatives with basophils from non-allergic subjects or subjects with unrelated allergies wer ...	1976	963864
search for a correlation between familial allergy to cocksfoot pollen and hla antigens.	twelve families comprising 62 individuals, 31 allergic to grasses, were examined. no significant correlation between pollinosis and the hla system was observed. however, there was a close correlation between cocksfoot skin tests and the rast (p less than 10(-6)).	1977	876127
[decomposition of grass-fructosanes by lactobacilli from silage (author's transl)].	lactobacilli isolated from grass silage were shown to belong to lb. plantarum, lb. casei and lb. brevis. in studies on the degradation of fructosanes significant differences among the three species were found. while all strains of l. casei were able to degrade fructosanes from dactylis glomerata or phleum pratense regardless of the degree of polymerization, no nember of lb. plantarum or lb. brevis could attack these polysaccharides.	1976	827143
enzyme determination and rast inhibition assays for orchard grass (dactylis glomerata): a comparison of commericial pollen extracts.	pollens release enzymes when they are dropped into water. orchard grass extracts were tested by two methods. the api-zym system determined 19 enzymes and colorimetric amounts of leucine aminopeptidases, acid phosphatase and trypsin. these were compared with rast inhibition assays. a good correlation was shown between the techniques. thus the enzyme dosage may be useful for the standardization of pollen extracts.	1978	686510
variations in the digestibility of trace elements (cu, zn and mn) during the first cycle of cocksfoot growth.	the variations of trace element availability was examined with 6 adult male sheep (texel) maintained in metabolic crates. they received cocksfoot cut each morning during the first vegetation cycle and offered ad libitum (10 % refused food), for 6 day periods. it appears from the resulting data that: -- the stage of cocksfoot development has a strong influence upon the digestibility and the retention of copper, zinc and manganese. --there is an almost perfect parallelism between the variations of ...	1977	606139
species specific grass pollen sensitivity: diagnosis and treatment with single grass species allpyral vaccines.	skin test titrations and nasal provocation tests in sixty patients with hay fever showed specific reactions to extracts of individual grass species. there was, however, no correlation between skin and nasal sensitivity. repeat testing after treatment with allpyral vaccines consisting of only the grass species to which the nasal reaction was most severe, or only one of several pollens to which reactions were equally severe, showed marked diminution of skin and nasal sensitivity not only to the si ...	1979	498489
an anomalous effect of allergen concentration on weal size elicited by skin testing for immediate hypersensitivity.	we report the finding of anomalous responses in the skin of atopic individuals when prick tested with serial dilutions of cocksfoot grass pollen extracts. each pollen extract was standardized using rast inhibition activity related to the who standard for ige. 75% of the patients gave a larger weal area with an allergen concentration of 1:10(5) than with the next higher concentration, i.e. 1:10(4). possible mechanisms whereby a larger skin weal is elicited with a lower concentration of allergen a ...	1979	421334
allergenic activity of fractions of cocksfoot (dacylis glomerata) pollen.	a cocksfoot (dactylis glomerata) pollen extract has been fractionated by isoelectric focusing in polyacrylamide gel, the separated components extracted from the gel and assayed by three methods: prick tests in human skin, rast and pca tests in monkey skin. prick testing in human skin showed that subjects responded differently to the separated components, indicating the presence of more than one antigenic determinant, and a profile of antibody activity against each fraction could be constructed. ...	1978	414856
cellular histamine release, specific and total serum ige levels in hay fever patients and controls.	the amount of histamine released from blood leucocytes by allergen, the amount of allergen required to release 50% histamine and the total ige and ige specific for dactylis glomerata are compared in nine hay fever patients and five control individuals. the variation with time of total ige, of specific igee and of the percentage of histamine released at a fixed allergen concentration, corresponding to the cellular sensitivity, is shown for one control individual with positive in vitro and skin te ...	1978	77195
fractionation of cocksfoot (dactylis glomerata) pollen by preparative isoelectric focussing.	the technique of isoelectric focussing in polyacrylamide gel has been developed to a preparative mode. using the improved technique cocksfoot (dactylis glomerata) pollen has been split into many components, and the allergenic reactions of the preparations have been studied by passive transfer in animal skin and by rast.	1978	23399