Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [detection and characteristics of defective interfering rna associated with cocksfoot mottle sobemovirus]. | 2008 | 10779963 | |
| rna-binding activities of cocksfoot mottle sobemovirus proteins. | cocksfoot mottle virus (cfmv) has a positive-sense ssrna genome containing four open reading frames (orfs). orf1 encoded protein (p1) is the putative movement protein; the product of orf2a (p2a) contains vpg and the motifs characteristic of serine proteases. p2b, encoded by orf2b, is the putative rna-dependent rna polymerase. p3, the coat protein, is encoded by orf3. cfmv p1, p2a, p2b, and p3, containing a six histidine tag at the amino terminus, were expressed in escherichia coli, purified and ... | 2000 | 10725552 |
| consequences of phenotypic plasticity vs. interspecific differences in leaf and root traits for acquisition of aboveground and belowground resources. | trade-offs between acquisition capacities for aboveground and belowground resources were investigated by studying the phenotypic plasticity of leaf and root traits in response to different irradiance levels at low nutrient supply. two congeneric grasses with contrasting light requirements, dactylis glomerata and d. polygama, were used. the aim was to analyze phenotypic covariation in components of leaf area and root length in response to above- and belowground resource limitation and the consequ ... | 2000 | 10719001 |
| chloroplast dna variation in dactylis glomerata l. taxa endemic to the macaronesian islands | in the dactylis glomerata infraspecific polyploid grass complex, restriction fragment length polymorphisms (rflps) of chloroplast dna (cpdna) were studied in diploid and tetraploid populations of several taxa endemic to macaronesia (madeira and the canary islands) and in populations from the african and european continental areas closest to macaronesia. two chlorotypes, which differed by a single 290-bp length mutation, were observed in the macaronesian and the continental populations. chlorotyp ... | 1999 | 10620224 |
| [investigations on the fermentation quality of silage from low-nitrate green forage]. | in 3-year-experiments green forage from dactylis glomerata and festuca species, produced by differentiated cutting frequencies, was ensiled under laboratory conditions. the forage was almost without nitrate. because of the higher content of water-soluble carbohydrates the fermentability coefficients of festuca species were above of dactylis glomerata. the fermentability coefficients of both plant species were inferior in the second and third cut, even if the first cut was delayed until july. des ... | 1999 | 10548978 |
| [in process citation] | the phyllochron of cocksfoot (dactylis glomerata l.) was studied over a 6-week period with two levels of nitrogen supply (0 and 150 kg ha-1) and three cutting regimes which mimic lenient (p), severe (s) and continuous (c) grazing. the phyllochron was highest for treatment with n supply and it decreased from the p to the c defoliation regimes. during the study period, the phyllochron and the sheath length throughout the successive leaves appeared to increase, except for the c treatments. these pa ... | 1999 | 10505244 |
| identification of genes encoding for the cocksfoot mottle virus proteins. | cocksfoot mottle sobemovirus (cfmv) has a monopartite single-stranded positive-sense rna genome. in wheat germ extract, in vitro translation of cfmv virion rna resulted in the production of four major proteins of 100, 71, 34 and 12 kda. in this paper we show the individual genes from which these polypeptides are synthesised. polyclonal antisera against the proteins encoded by all open reading frames (orfs) of cfmv were produced. antibodies were used to immunoprecipitate the in vitro translation ... | 1999 | 10486110 |
| inhibition of the seasonal ige increase to dactylis glomerata by daily sodium chloride nasal-sinus irrigation during the grass pollen season. | 1999 | 10482855 | |
| substitution of mainly lysyl groups of the proteins in an extract of dactylis glomerata by succinic and maleic anhydrides. | 1999 | 10442536 | |
| [antigen specific immunoglobulin e to grass and weed pollens in the plasma of patients with seasonal allergic rhinitis]. | the study involved 22 patients with seasonal allergic rhinitis between 13 and 53 years of age. the level of antigen specific ige (as ige) to 5 grass and 3 weed pollens was determined with the use of cap feia (pharmacia, uppsala, sweden). the control group consisted of 20 persons. all above as ige were significantly higher in the patients with seasonal allergic rhinithis than in the control group. the most commonly present hypersensitivities were to meadow fescue (festuca elatior), meadow grass ( ... | 1999 | 10337158 |
| physicochemical and immunologic characterization of low-molecular-weight allergoids of dactylis glomerata pollen proteins. | orchard grass (dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins. | 1999 | 10221435 |
| environmental priming influences allergen-specific nasal reactivity. | preceding mucosal response to one allergen leads to the priming of the nasal mucosal response to another allergen. this study aimed to determine whether environmental allergens, especially ubiquitous animal dander, can induce nasal priming. | 1998 | 9930593 |
| [specific skin reactions induced by individual pollen preparations in hypersensitivity persons]. | skin tests are the most useful single modality for demonstrating an ige-mediated mechanism underlying clinical symptoms. skin reactivity to an allergen depends on person's exposure and genetic factors influencing the ige response. however, the reproducibility of allergy skin tests has been shown to be variable depending, among other things, on the allergen extract employed and the kind of technique used. these variations have led to controversy regarding clinical relevancy of allergy skin testin ... | 2009 | 9863408 |
| supplementation of rumen-undegradable protein to the diets of early lactation holstein cows on grass pasture. | the objectives were to evaluate supplements differing in rumen-undegradable protein (rup) on the performance of early lactation cows on grass pasture. twenty-four holstein cows averaging 68 d of lactation and 39.8 kg/d of milk were rotationally grazed on predominantly dactylis glomerata pasture for 8 wk. cows were blocked according to parity, milk yield, and days of lactation and were randomly assigned to a grain mixture with a low or high rup content. pasture and the low and high rup grain mixt ... | 1998 | 9749386 |
| how far can we simplify in vitro diagnostics for grass pollen allergy?: a study with 17 whole pollen extracts and purified natural and recombinant major allergens. | current diagnostics for grass pollen allergy are composed of mixtures of pollen of different grass species. their complex composition hampers accurate standardization. | 1998 | 9723659 |
| detection and quantification of group 4 allergens in grass pollen extracts using monoclonal antibodies. | grass pollen extracts are complex mixtures consisting of different major allergenic and non-allergenic components. phl p 4 is an important allergen, because more than 75% of grass pollen allergic patients produce specific ige antibodies against group 4 allergens. | 1998 | 9720813 |
| effect of plant species on the larvae of gastrointestinal nematodes which parasitise sheep. | faeces containing trichostrongylus colubriformis and/or ostertagia circumcincta eggs were used to provide four contaminations in each of 2 years on plots of browntop, yorkshire fog, ryegrass, tall fescue, lucerne, chicory, cocksfoot, white clover, and prairie grass and in the second year a mixed sward of ryegrass/white clover. third stage larvae were recovered from faeces and from four strata of herbage, 0-2.5, 2.5-5, 5-7.5 and > 7.5 cm above the soil surface at 2, 4, 6, 8, 11, and 14 weeks afte ... | 1998 | 9650060 |
| effect of extreme walking conditions for dairy cows on milk yield, chemical composition, and somatic cell count. | thirty-two cows (16 montbeliardes and 16 tarentaises) in midlactation were used in an experiment utilizing a 2 x 2 factorial arrangement of treatments. throughout the trial, cows received first-cutting cocksfoot hay for ad libitum intake supplemented with a fixed amount of concentrate that was individually adapted to the milk yield of each cow. during a 23-d experimental period, one group of cows walked 9.6 km/d; the other group of cows remained in the barn. cows that walked daily ate less hay ( ... | 1998 | 9594388 |
| variability in the pattern of random amplified polymorphic dna. | the random amplified polymorphic dna (rapd) technique is a simple method to detect dna polymorphism. it is sensitive to reaction conditions. small changes in the reactants' concentration cause variations in amplification products. using dna from asparagus officinalis, dactylis glomerata, mercurialis annua and escherichia coli, we examined variability in the amplification pattern associated with reaction constituents. an increase in the ratio of taq dna polymerase to dna in the reaction increased ... | 1997 | 9504820 |
| effects of dietary supplementation on nutrient digestion and the milk yield of intensively grazed lactating dairy cows. | fourteen midlactation holstein cows were used in an 80-d study to examine supplementation strategies during intensive rotational grazing. factors examined were the concentration of protein in the supplement [12 or 16% crude protein (cp) on a dry matter basis] and the amount of supplement offered (6.4 or 9.6 kg/d per cow). the supplement was offered in equal portions three times daily after milking. pasture, composed of alfalfa (medicago sativa) and orchardgrass (dactylis glomerata), was divided ... | 1997 | 9436110 |
| evaluation of a mobile computerized grain feeder for lactating cows grazing grass pasture. | the objective of this study was to evaluate a mobile computerized grain feeder for use to feed individually holstein cows grazing grass pasture. thirty-two holstein cows averaging 95 d of lactation and 39.3 kg/d of milk were rotationally grazed on predominantly dactylis glomerata pastures for 9 wk starting in early may. cows were blocked according to parity, days of lactation, and milk yield. cows were randomly assigned to a control group in which cows were individually fed grain twice daily at ... | 1997 | 9436109 |
| genetic structure of an aphid studied using microsatellites: cyclic parthenogenesis, differentiated lineages and host specialization. | in a previous study, samples of the grain aphid sitobion avenae (f.) were collected from wheat and adjacent cocksfoot hosts in a population thought to be primarily parthenogenetic, and dna from individual aphids was analysed with a multilocus technique. here we have applied single-locus microsatellites and a mitochondrial dna marker to a subset of the same dna extracts, and have made several additional inferences about important genetic and population processes in s. avenae. microsatellite analy ... | 1997 | 9394464 |
| ruminal undegradability of blood meal and effects of blood meal on ruminal and postruminal digestion in steers consuming vegetative orchardgrass hay. | four crossbred steers (360 +/- 3 kg) cannulated at the rumen and duodenum were used in a 4 x 4 latin square design to examine the effects of supplemental blood meal (bm) on voluntary intake, digesta kinetics, ruminal fermentation, site and extent of digestion, and bacterial protein synthesis in steers fed vegetative orchardgrass hay (dactylis glomerata l.). the levels of bm supplementation were 0, .07, .13, and .20 kg/d. voluntary intake of om (8.35 kg/d) was not significantly affected by bm sup ... | 1997 | 9331885 |
| gramineae pollen as trigger factors of atopic eczema: evaluation of diagnostic measures using the atopy patch test. | after contact with grass pollen, seasonal exacerbations of eczematous skin lesions have been described in a subgroup of patients with atopic eczema (ae). epicutaneous patch testing with aeroallergens (atopy patch test. apt) has been used to investigate these patients. we performed comparative apt in 79 patients with ae and 20 control subjects (14 non-atopic volunteers and six patients with grass pollen allergic rhinoconjunctivitis). subjects were tested with grass pollen allergen extract in petr ... | 1997 | 9292067 |
| cross-reactivity to olive tree pollen and orchard grass pollen in patients with pollinosis. | we studied 92 patients with allergic rhinitis in syodoshima, japan, during the pollen season between april and june to evaluate the cross-reactivity to different antigens, including pollen from the olive tree (olea europaea) and orchard grass (dactylis glomerata). olive tree pollen was present in the atmosphere for 23 days, from may 19 to june 12, 1994. specific ige antibodies for olive tree pollen antigen were present in 21 (26.9%) of the 78 patients with allergic rhinitis. nine (24.3%) of the ... | 1997 | 9227797 |
| efficacy of intranasally applied dimethindene maleate solution as spray in adult volunteers with symptoms of seasonal allergic rhinitis in the vienna challenge chamber. | twelve asymptomatic subjects (4 female, 8 male), being allergic to grass pollen proved by positive anamnesis, positive prick-test and positive nasal provocation test, were challenged under controlled conditions with purified airborne grass pollen of dactylis glomerata in the vienna challenge chamber (vcc), located at the universitätsklinik für hals-nasen-ohrenheilkunde, allgemeines krankenhaus (akh), der stadt wien, vienna (austria) by means of a double-blind, randomised, cross-over design, with ... | 1996 | 9125281 |
| kinetics of cell-wall digestion of orchardgrass and alfalfa silages treated with cellulase and formic acid. | the objectives of this study were to determine the effects of cellulase (from trichoderma longibrachiatum) combined with formic acid, applied before ensiling, on the subsequent concentration and composition of the cell wall and on the extent and rate of in situ cell-wall digestion of orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.). treated and control forages of both plant species were ensiled for at least 60 d before being ruminally digested by two fistulated cows. analyse ... | 1996 | 9029359 |
| an immunoblotting analysis of cross-reactivity between melon, and plantago and grass pollens. | it is known that most patients with type i allergy to pollens also suffer intolerance to fruits. recently, an epidemiological and cap-inhibition study has shown a new clustering of allergy between melon and plantago and grass pollens. the aim of the present study was to confirm these results by immunoblotting analysis and inhibition of immunoblotting. sera from 3 patients with confirmed allergy to melon, and dactylis glomerata and plantago lanceolata pollens were used for the in vitro studies. s ... | 2013 | 9015782 |
| characterization of dac g 4, a major basic allergen from dactylis glomerata pollen. | monoclonal antibodies were produced against dac g 4, a purified major basic allergen from dactylis glomerata pollen. their ability to be used for immunopurification of dac g 4 was studied on a biacore apparatus (pharmacia). the allergen was purified by affinity chromatography with one monoclonal antibody. its precise molecular mass, 59,185 +/- 30 d, was determined by mass spectrometry. its isoelectric point is 10.4. sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting show ... | 1996 | 8977507 |
| isotypic analysis of grass pollen-specific antibodies in human plasma. 4. biological activity of allergen-specific and autoanti-ige antibody fractions on basophil histamine release. | blocking antibodies are defined as antibodies that compete with ige for binding to allergens due to their specificity for those allergens. thus, they may inhibit allergen-induced basophil and mast cell ige-dependent mediator release both in vivo and in vitro. | 1996 | 8955580 |
| determination of sensitivity of inhalant allergens in patients with allergic rhinitis in west athens. | in this study we have determined the sensitivity to inhalant (pollen and non-pollen) allergens in patients with allergic rhinitis from west athens, greece. the frequency of spt positivity was highest for dactylis glomerata, parietaria, olea europea, dermatophagoides and several moulds. | 1995 | 8919218 |
| [olive pollinosis in japan]. | olive (olea europaes) is the most important allergic tree in the mediterranean area. that is widely distributed at syodoshima in japan. i observed olive pollination period and studied the patients with pollinosis. the patients were examined by skin test, measuring of specific ige antibody and nasal provocation test with olive pollen extract. allergic responses to olive pollen were found to be present in 16.3% of pollinosis patients. and cross-reactivity between olive and grass pollen (dactylis g ... | 1995 | 8857110 |
| cloning, sequencing and immunological characterization of dac g 3, a major allergen from dactylis glomerata pollen. | preliminary work showed that a 14-kda allergen with a pi of 9 was recognized by more than 60% of sera from dactylis glomerata (dac g) pollen-allergic individuals. the n-terminal amino acid sequence of this dac g allergen was determined by edman degradation and compared with that of lol p 3, a major allergen of lolium perenne. a sequence identity of 65% was found, suggesting that the dac g allergen could be the homologue of lol p 3 and therefore named dac g 3. we report the cloning and sequence a ... | 1996 | 8811075 |
| size-exclusion liquid chromatography and capillary electrophoresis of pollen allergens. | allergens from the pollen of phleum pratense, dactylis glomerata. arrhenatherum elatius, secale cereale, lolium perrene and festuca sp. were analysed by size-exclusion chromatography (sec) and capillary electrophoresis (ce). sec was used for the determination of the molecular masses of main allergens. a ce method, using either 150 mmol/l phosphoric acid (ph 1.8) or a micellar system consisting of 50 mmol/l sodium dodecyl sulphate-20 mmol/l borate (ph 9.35), was developed as a rapid and efficient ... | 1996 | 8798911 |
| influence of barley and buffer supplements on quantitative aspects of ruminal fiber digestion of cows. | four jersey cows, fitted with ruminal and duodenal cannulas, were used to assess the specific effects of changes in ruminal ph that were induced by barley addition to a hay diet on ruminal fiber digestion. cows received successively 100% unchopped cocksfoot hay (diet h), 65% hay and 35% pelleted ground barley (diet hb), and hb plus a continuous intraruminal infusion of bicarbonate salt solution (diet hbb). cows were limit-fed once daily at 7 kg of dm day-1. the ruminal fractional passage rate of ... | 1996 | 8767054 |
| [sobemovirus genome from dactylis glomerata has a luteovirus-like organization of the replicase and protease gene modules]. | 2008 | 8754004 | |
| influence of sampling time and diet on amino acid composition of protozoal and bacterial fractions from bovine ruminal contents. | four ruminally cannulated cows were used to compare amino acid (aa) composition of protozoal and bacterial fractions as affected by sampling time and diet. cows were given once a day restricted feed (80% of ad libitum intake) of 7 kg dm with two successive diets. diet hb was 65% cocksfoot hay and 35% pelleted ground barley, and diet h was 100% cocksfoot hay. samples of whole ruminal contents were taken 2, 5, 8, 11, and 23 h after feeding for diet hb and 2 h after feeding for diet h to isolate th ... | 1996 | 8726749 |
| forage systems for beef production from conception to slaughter: iii. finishing systems. | fall-weaned angus calves grazed or were fed different forages during winter followed by 1) n-fertilized tall fescue (festuca arundinacea schreb.) grazed alone, 2) bluegrass (poa pratensis l.)-white clover (trifolium repens l.) sequence grazed with tall fescue-red clover (trifolium pratense l.), or 3) bluegrass-white clover sequence grazed with alfalfa (medicago sativa l.)-orchardgrass (dactylis glomerata l.). heifers were supplemented with grain at 1% of bw from april until slaughter in july. on ... | 1996 | 8707721 |
| spatial and temporal genetic variation in british field populations of the grain aphid sitobion avenae (f.) (hemiptera: aphididae) studied using rapd-pcr. | the grain aphid sitobion avenae (f.) was collected from winter wheat and adjacent cocksfoot grass at two locations in southern england and at four times in the year (april-july). genetic variation between individual aphids was then investigated using random amplified polymorphic dna polymerase chain reaction. individuals caught in wheat and cocksfoot during april provided very different and highly diagnostic banding patterns that were independent of location. this host-based genetic differentiab ... | 1995 | 8587889 |
| t-cell epitopes of phl p 1, major pollen allergen of timothy grass (phleum pratense): evidence for crossreacting and non-crossreacting t-cell epitopes within grass group i allergens. | the use of peptides representing t-cell epitopes of allergens is a modern concept for improvement of specific immunotherapy. a prerequisite for this approach is the identification of t-cell epitopes of atopic allergens. | 1995 | 8543758 |
| allergenic relationship between taxonomically diverse pollens. | skin tests and tests for ige antibodies show that subjects are usually sensitive to a number of different pollens, frequently from taxonomically diverse species which are assumed to be allergenically non-crossreactive. this suggests that the presence of ige antibody-reactivity to an individual pollen may not necessarily have resulted from contact with that pollen or even with a taxonomically closely related species. | 1995 | 8521178 |
| isotypic analysis of grass pollen-specific immunoglobulins in human plasma. 1. specialization of certain classes and subclasses in the immune response. | the specificity and isotypic profile of humoral immune responses to dactylis glomerata (cocksfoot) pollen was studied by isoelectric focusing (ief)-immunoprint analysis using 26 human plasma samples with high levels of dactylis pollen-specific igg4 (igg4+ plasma) and 25 human plasma samples with low levels of specific igg4 (normal plasma). over 60 individual protein components in an aqueous pollen extract were separated by ief and immunoprinted onto nitrocellose (nc). following plasma incubation ... | 1993 | 8428167 |
| in vivo assessment of mast cell functional alteration induced by l-leucine methyl ester, using the passive cutaneous anaphylaxis technique. | we have recently reported that in vitro, mast cells were sensitive to the action of l-leucine methyl ester (leu-ome), a lysosomotropic compound. we now report the in vivo effect of leu-ome on mast cells, qualitatively assessed by using the passive cutaneous anaphylaxis (pca) reaction. the l- but not the d-stereoisomer of leu-ome (25 mm) injected together with dactylis glomerata, pollen-specific, ige-containing serum inhibited the pca reaction in rat skin triggered by a subsequent challenge with ... | 1993 | 8428165 |
| purification of human igg4 subclass with allergen-specific blocking activity. | blocking antibodies (bab) induced by allergen immunotherapy are restricted to the igg1 and igg4 subclasses, with igg1 predominating early and igg4 coming later. study of igg4 bab has been limited, in part, by the absence of a method to purify igg4. we describe a rapid immunoaffinity chromatographic method for the purification of that subclass from whole serum. starting serum (tr) contained 90 micrograms/ml dactylis glomerata (orchard grass) pollen (dgp)-specific igg4, measured by indirect elisa. ... | 1993 | 8409473 |
| two-dimensional gel electrophoretic analysis with immobilized ph gradients of dactylis glomerata pollen allergens. | we studied orchard grass pollen allergens (dactylis glomerata) by a new two-dimensional electrophoretic technique which is the combination of isoelectric focusing (ief) in immobilized ph gradient (ipg) and horizontal sds-page. the ipg-dalt (two-dimensional gel electrophoresis with immobilized ph gradients in the first dimension) electrophoresis was made with four different but complementary ph gradients: 3-10.5, 4-9, 4-7, 6.3-10.5. we also compared the behavior of allergens in native and denatur ... | 1993 | 8400888 |
| dmisa (dissociated membrane immunosorbent assay), a new elisa technique performed with blotted samples. | this study describes the use of electrophoretically purified antigens blotted onto nitrocellulose, as solid phase antigens for enzyme-linked immunosorbent assays. this procedure is called dmisa, for dissociated membrane immunosorbent assay. the method is illustrated using immunoblotted antigens of dactylis glomerata grass pollen extract. the band of interest was located on a print of nitrocellulose by light staining (india ink), then the corresponding strip of nitrocellulose was cut out. immedia ... | 1994 | 8308278 |
| an integrated, dynamic model of feed hydration and digestion, and subsequent bacterial mass accumulation in the rumen. | hydration of feeds and bacterial attachment to feed particles are thought to play major roles in rumen digestion of fibrous feedstuffs. the objective of the present study was to integrate these phenomena in a mechanistic model that could be used for data analysis. the proposed model was based on the conversion of biomass, where digestion end-products can be used for the synthesis of bacterial mass. digestion of the potentially digestible fraction and subsequent accumulation of bacterial mass was ... | 1993 | 8260474 |
| influence of forage species and diet particle size on the passage of digesta and nylon particles from the reticulorumen of steers. | to assess factors that influence the passage of digesta from the reticulorumen, ruminally fistulated steers (387 kg) were fed to appetite in a 4 x 4 latin square design either orchardgrass (og) (dactylis glomerata l.) or switchgrass (sg) (panicum virgatum l.) hays. the two hays were fed in either the long form or ground to pass a 2-cm screen. steers fed og diets had greater dmi (p < .05) than steers fed sg diets (11.7 vs 10.4 kg/d respectively). particle size of ruminal digesta and feces determi ... | 1993 | 8226378 |
| digestibility, nitrogen utilization, and voluntary intake of ensiled crab waste-wheat straw mixtures fed to sheep. | crab waste and wheat (triticum aestivum l.) straw mixtures, ensiled with different additives, were evaluated in metabolism and palatability trials. crab waste and straw were mixed in proportions of 1:1, wet basis, with 20% water and different additives, and ensiled in 210-l metal drums double-lined with polyethylene bags. thirty crossbred wethers (40 kg initial bw) were fed a 1) basal diet consisting of 75% orchardgrass (dactylis glomerata l.) hay and 25% concentrate, 2) ensiled crab waste-wheat ... | 1994 | 8181970 |
| nucleotide sequence of cdna encoding the group ii allergen of cocksfoot/orchard grass (dactylis glomerata), dac g ii. | cocksfoot/orchard grass (dactylis glomerata) anther cdna clones encoding the group ii allergen dac g ii were previously isolated on the basis of immunoreactivity of human, rabbit, and murine antibodies with a 24-kda protein expressed as a fusion protein with beta-galactosidase. nucleotide sequencing reveals an open reading frame predicting expression of a 98-amino-acid (11-kda) polypeptide exhibiting > 90% homology with the group ii allergen of lolium perenne, lol p ii. in vitro translation of d ... | 1993 | 8116860 |
| comparison of three horizontal two-dimensional electrophoretic techniques to separate grass pollen allergens. | the identification and characterization of allergenic components are important for improving both diagnosis and therapy of allergy. we have studied grass pollen crude extract for better characterization of the allergen repertoire recognized by allergic patient ige antibodies. two-dimensional electrophoresis are the methods of choice to define physico-chemical characteristics of the allergens as they give isoelectric points and molecular mass of the analyzed samples. we compared the advantages an ... | 1994 | 8003933 |
| analysis of human t cell clones reactive with group v grass pollen allergens. | twenty-seven t cell clones (tcc) reactive with group v allergens of phleum pratense (phlp v) were established from the peripheral blood of 3 patients allergic to grass pollen. twenty-four tcc showed the helper cell phenotype cd3+, cd4+, cd8-; the remaining clones were cd3+cd4-cd8+. t cell recognition of phlp v was exclusively hla-dr restricted. many of the phlp v reactive tcc (19 of 27; 70%) were stimulated additionally by other group v allergens isolated from lolium perenne, poa pratensis, and ... | 1994 | 7981611 |
| evaluation of brassicas in grazing systems for sheep: i. quality of forage and animal performance. | four years of grazing trials were conducted with brassica forages to evaluate their chemical composition and effect on adg of fattening lambs and breeding ewes in late fall. brassicas tested included kales (brassica oleracea l. var. acephala dc), turnips (b. rapa l.), and a chinese cabbage hybrid (b. rapa l. x b. pekinensis [lour.] rupr.). daily gains of lambs varied widely among years (19 to 330 g/d); adg on brassicas were, however, generally higher than on stockpiled kentucky 31 tall fescue (f ... | 1994 | 7928762 |
| seasonal asthma caused by airborne platanus pollen. | this work describes three patients suffering from bronchial asthma after being naturally exposed to airborne plane-tree pollen. the three patients gave immediate response in skin tests and dual response in bronchial provocations using platanus hybrida extract. there was specific seric ige activity against this/these antigen(s) with the cap system. the three patients also showed significant correlation (p < 0.001) between their rhinitis and asthma symptom-scores registered on their diary cards an ... | 1994 | 7889425 |
| the putative replicase of the cocksfoot mottle sobemovirus is translated as a part of the polyprotein by -1 ribosomal frameshift. | the polyprotein of cocksfoot mottle sobemovirus (cfmv) is encoded by two overlapping open reading frames (orf). the orf 2a codes for the putative vpg and serine protease and the orf 2b codes for the putative replicase. the consensus signals for a -1 ribosomal frameshifting event are found at the very beginning of the overlapping region of these orfs. the shifty heptanucleotide in cfmv is uuuaaac, and the secondary structure after the shifty sequence is predicted to be a stem-loop. in vitro trans ... | 1995 | 7886961 |
| isolation and characteristics of the protozoal and bacterial fractions from bovine ruminal contents. | four cows were fed once a day either a cocksfoot hay diet (h) or a diet consisting of 65% hay and 35% pelleted ground barley (hb). 15(nh4)2so4 was continuously infused into the rumen as a microbial marker and ruminal digesta samples were collected during the 24-h postprandial period for the isolation of liquid-associated protozoa and bacteria (lap, lab) and particle-associated bacteria (pab). there were marked differences between ruminal ph diurnal variations with diets h and hb. irrespective of ... | 1994 | 7730192 |
| replication and encapsidation of the viroid-like satellite rna of lucerne transient streak virus are supported in divergent hosts by cocksfoot mottle virus and turnip rosette virus. | cocksfoot mottle sobemovirus supports replication and encapsidation of the viroid-like satellite rna (sat-rna) of lucerne transient streak virus (ltsv) in two monocotyledonous species, triticum aestivum and dactylis glomerata. additionally, ltsv sat-rna replicates effectively in the presence of turnip rosette sobemovirus in brassica rapa, raphanus raphanistrum and sinapsis arvensis, but not in thlaspi arvense or nicotiana bigelovii, indicating that host species markedly influence this interactio ... | 1993 | 7682254 |
| grass pollen immunotherapy induces highly cross-reactive igg antibodies to group v allergen from different grass species. | sera from two groups of patients receiving grass pollen immunotherapy were tested on igg reactivity with group v allergen from six different grass species. one group of patients was treated with a mixture of 10 grass species, and the other with a mixture of five. only lolium perenne, dactylis glomerata, and phleum pratense were present in both mixtures. although anthoxanthum odoratum and secale cereale were absent from the mixture of five, igg responses to ant o v and sec c v were comparable in ... | 1995 | 7677246 |
| optimization and characterization of freeze-dried multilamellar liposomes incorporating different standardized allergen extracts. | desensitization therapy for type i allergy is now current practice. liposomes have been proposed as a support for allergens to improve safety and effectiveness. the aim of this work was to optimize liposomal formulations of three different standardized allergen extracts and to test their allergenicity in vitro and in a preclinical trial. allergen extracts (dactylis glomerata, dermatophagoides pteronyssinus, and cat hair and dander) were associated with multilamellar liposomes of varying composit ... | 1994 | 7653744 |
| characterization of cocksfoot mottle sobemovirus genomic rna and sequence comparison with related viruses. | the genome of cocksfoot mottle virus (cfmv) is a positive-sense ssrna molecule of 4082 nucleotides as revealed by sequencing the entire genome. the 5'-untranslated region of the genome is 69 nucleotides and the 3'-untranslated region is 225 nucleotides in length. the coding region contains four open reading frames (orfs). the organization of cfmv orfs differs significantly from that of the previously sequenced sobemoviruses southern bean mosaic virus and rice yellow mottle virus. orf1 encodes a ... | 1995 | 7595389 |
| comparative studies of allergenic extracts prepared from freshly collected, dried, or dried and defatted pollen. | fresh pollen samples of parietaria judaica, betula pubescens, dactylis glomerata and olea europaea were collected and aliquot portions of these pollens were stored after drying in hot air, while other portions were dried and then defatted with diethylether. the different pollen materials were extracted with aqueous media to obtain the non-dialyzable allergenic protein constituents. review of the production data, together with comparative analyses of the uv-absorption spectra and of ige binding p ... | 1995 | 7582159 |
| [the monitoring of natural populations of dactylis glomerata l. in the area of the accident at the chernobyl atomic electric power station]. | results of five years monitoring (1986-1991) of seed quality (sq) and chlorophyll mutation frequency (mf) of dactylis glomerata l., growing within 30-km zone of chernobyl npp are presented. it was shown that during initial postaccident period (1986, 1987) the criteria depend of the gamma-phone on the sampling plots. it was not found the correlation between sq, mf and duration of plant growing under condition chronic irradiation. | 2016 | 7489103 |
| ige antibody formation in balb/c mice without adjuvant: induction of responses to grass pollen extract and to a hapten-carrier conjugate. | the ige antibody responses of inbred balb/c mice to subcutaneously injected antigens, given without adjuvant, were studied. cocksfoot grass pollen extract (gpe) (100-100,000 pnu) induced no detectable primary ige antibody responses but following second and subsequent injections, substantial secondary responses were produced. weekly injections for 9 weeks of doses of doses of 0.1-10,000 pnu gpe all produced similar ige antibody titres as measured by 24 h passive cutaneous anaphylaxis (pca) in rat ... | 1980 | 7380480 |
| the relationship of certain tymoviruses assessed from the amino acid composition of their coat proteins. | the amino acid composition of the coat proteins of the following viruses is reported: andean potato latent, clitoria yellow vein, desmodium yellow mottle, dulcamara mottle, eggplant mosaic, okra mosaic, ononis yellow mosaic, scrophularia mottle, and, as controls, cocksfoot mild mosaic and cocksfoot mottle viruses. these data, together with some already published, were used to compute classifications of the tymoviruses. these classifications show a general similarity to koenig's serological class ... | 1980 | 7372445 |
| allergenic activity of fractions of cocksfoot (dactylis glomerata) pollen. definition of active components by skin testing, and inhibition rast. | with the combined use of skin tests, rast and isoelectric focusing, a potency profile of a cocksfoot ((dactylis glomerata) pollen extract was prepared. it was found that two fractions with pi 4.5 and 6.0 were the most allergenic, had the highest protein concentration and were also the most easily extracted. the importance of converting mean weal sizes into potency units when comparing the allergenicity of pollen fractions is emphasized. | 1981 | 7249343 |
| [evolution of microflora during the conservation of forage in microsilos]. | fresh and wilted samples of lolium italicum a. br., dactylis glomerata l., medicago sativa l., have been ensiled in laboratory microsilos for the study of microflora evolution. the principal microbial groups (coliforms, proteolytic bacteria, clostridia, lactic acid bacteria, yeasts) have been detected and enumerated, in anaerobic atmosphere, after 8, 15, 22, 29, 36, 180 days of ensiling. although the number of lactic acid bacteria resulted to the high in all the samples studied, they failed to p ... | 2006 | 7187351 |
| grass pollen hyposensitization versus placebo therapy. i. clinical effectiveness and methodological aspects of a pre-seasonal course of desensitization with a four-grass pollen extract. | the present work reports the results of a double-blind clinical trial, comparing the effects of hyposensitization treatment versus placebo in 33 patients with allergic rhinitis, sensitive to a crude extract of the pollen of four different grasses (dactylis glomerata, lolium perenne, secale cereale, and phleum pratense). the distribution of these patients in the two groups was done randomly and gave two comparable groups, as far as clinical and biological features are concerned. the treatment cou ... | 1981 | 7032341 |
| [reduced allergen immunotherapy of grass pollinosis (author's transl)]. | an excellent model for investigating the maximization of specific immunotherapy is grass pollen allergy. treatment is more effective when the number of component antigens used are reduced. we know that rye and cocksfoot pollen carry major antigens of grass pollen. a controlled clinical study was carried out with 128 patients divided into 5 groups as follows: group 1 - treatment with a mixture of 7 grass pollen extracts (allpyral). group 2 - treatment with a cocksfoot pollen extract (allpyral). g ... | 1980 | 6936982 |
| inhibition of the passive cutaneous anaphylaxis reaction to dactylis glomerata pollen allergens by a purified component of this pollen. | by isoelectric focusing a protein fraction of pi 4.6 was isolated from a crude water-soluble extract of dactylis glomerata pollen (se). this fraction was neither immunogenic nor allergenic in balb/c mice. in one week, this protein inhibited the mouse ige-specific antibodies to the soluble extract as measured by pca in rats and was therefore called dactylis inhibitory protein (dip). two experimental approaches which lowered ige anti-se titer were undertaken. pretreatment with dip as well as injec ... | 1983 | 6885115 |
| performance of dairy cows in midlactation fed high quality grass pasture and concentrate at three percents of energy requirements. | thirty multiparous cows rotationally grazed high quality grass pastures (predominantly dactylis glomerata and poa pratensis) for 140 days. stage of lactation was 54 to 192 days at beginning of trial. cows were offered concentrates to supply 33, 66, or 100% of requirements for net energy for lactation above maintenance; daily intakes by the three groups averaged 3.5, 7.7, and 11.5 kg. refusal of concentrate tended to increase with supplementation. average milk production was 19.2, 20.4, and 21.5 ... | 1983 | 6841749 |
| heterogeneity of grass pollen allergens (dactylis glomerata) recognized by ige antibodies in human patients sera by a new nitrocellulose immunoprint technique. | a new nitrocellulose immunoprint technique has been developed to detect specific antigens or/and allergens present among a heterogeneous solution such as a water-soluble crude extract of a grass pollen (dactylis glomerata). the antigens are separated by isoelectric focusing (ief) in an agarose gel and characterized by their isoelectric point (pi). these antigens are transferred and immobilized on a nitrocellulose sheet. they are recognized by the binding of specific antibodies contained in an un ... | 1982 | 6759375 |
| immunotherapy with alginate-conjugated and alum-precipitated grass pollen extracts in patients with allergic rhinoconjunctivitis. | in order to compare the safety and the efficacy of two grass pollen extracts made from the same starting material, i.e. equal proportions of cocksfoot and timothy, a 3-year study was made on patients with hay fever. one product was a dialysed aqueous extract of pollens which was chemically conjugated to activated sodium alginate ( conjuvac ). the other was a pyridine-extracted alum-precipitated crude extract (allpyral). forty patients with classical hay fever were admitted to the study. all gave ... | 1984 | 6731753 |
| a collaborative study on rast inhibition using a common method. | a collaborative study was carried out to determine the reproducibility of the inhibition form of the radioallergosorbent test (rast). seven laboratories were provided with three coded extracts of cocksfoot (dactylis glomerata) pollen. all laboratories used a common method of rast inhibition and were supplied with all reagents required to carry out the assays. in addition, four laboratories used local methods of rast inhibition. potency estimates were in reasonable agreement between laboratories ... | 1984 | 6697468 |
| a report on the consumption, composition and nutritional adequacy of a mixture of lush green perennial ryegrass (lolium perenne) and cocksfoot (dactylis glomerata) fed ad libitum to thoroughbred mares. | non-pregnant thoroughbred mares were stabled and subjected to 2 trials, each 24 h in duration, to establish their total consumption of a mixture of freshly cut, lush green perennial ryegrass (lolium perenne) and cocksfoot (dactylis glomerata) in approximately equal proportions; and to compare the total intake of crude protein, calcium, phosphorus and mass of the grass mixture on a dry matter basis with their daily nutritional requirements. the body mass of each mare was calculated at the commenc ... | 1983 | 6655653 |
| alkaloids in ergot found on different gramineae in the netherlands. | the alkaloid content and the composition of the alkaloid complex of thirteen samples of ergot sclerotia from different gramineous host species collected in the netherlands were investigated. two samples collected in france were also examined. ergot of glyceria fluitans (l.) r.br. did not contain alkaloids. the total alkaloid content of ergot found on the other wild grasses was more than 0.2%. the ratio between the contents of water soluble and water insoluble alkaloids of the investigated ergot ... | 1983 | 6646990 |
| investigations of the allergens of cocksfoot grass (dactylis glomerata) pollen. | the pollen of cocksfoot grass (dactylis glomerata) is an important cause of allergic reactions in man. preliminary studies, which established that constituents of an extract of this pollen could be separated, and then recovered efficiently, by size-exclusion chromatography on tsk g3000 sw, have been extended. a comparative examination has been made by this procedure of cocksfoot pollen extracts from different sources and of several batches of extract from one source. the recovery and distributio ... | 1983 | 6630355 |
| isopycnic centrifugation of plant viruses in nycodenz density gradients. | isopycnic centrifugation of plant viruses in density gradients prepared from a new non-ionic medium (nycodenz) was investigated. particle density of luteo-, tymo-, nepo-, cocksfoot mild mosaic, tobamo-, hordei-like, potex- and potyviruses in buffered solutions of nycodenz ranged between 1.23 and 1.28 g/ml and did not strictly reflect their nucleic acid and protein composition. isopycnic centrifugation of several partially purified viruses yielded preparations which were pure by electron microsco ... | 1984 | 6520198 |
| presence of il 1 activity in the supernatant of human monocytes challenged with purified allergenic constituents. | purified allergenic constituents from dermatophagoides farinae mite (df 11 and df 6) and from orchard grass (dactylis glomerata) pollen (f 34) are able to trigger, in the absence of ige and igg antibodies, human adherent cells, leading to the secretion of interleukin 1, (il 1). this secretion was observed with adherent peripheral blood mononuclear cells from normal donors as well as from atopic patients. the il 1 activity was evaluated by the mice thymocyte mitogenesis in the presence of subopti ... | 1984 | 6332080 |
| mineral status of beef cows and sheep on spring pasture fertilized with kieserite. | the utilization of minerals in orchardgrass (dactylis glomerata l.) fertilized with kieserite (2,240 kg/ha) was examined in spring with lactating beef cows and wether lambs. kieserite increased the concentration of mg in vegetative herbage from .22 to .29% ( p less than .01) and of s from .20 to .29% (p less than .01), with little effect on other minerals. fertilization also increased (p less than .01) apparent absorptions of mg and s by lambs fed cut orchardgrass, from 24.5 to 36.5% and from 58 ... | 1981 | 6263852 |
| metabolic inhibitor(s) in fractions of orchardgrass (dactylis glomerata l.) detected by in vitro rumen fermentation technique. | 1967 | 6024807 | |
| the energy value of ryegrass and cocksfoot assessed by a slaughter technique with lambs. | 1965 | 5835395 | |
| metabolic inhibitor(s) in orchardgrass (dactylis glomerata l.). | 1968 | 5690265 | |
| [the occurrence of the cocksfoot streak virus]. | 2016 | 4912699 | |
| cocksfoot mild mosaic virus--phleum mottle virus: a comparison. | 1974 | 4851066 | |
| [enzootic calcinosis in cattle. 5. experimental studies on rabbits selectively fed with ball-grass (dactylis glomerata), gold-oats (trisetum flavescens) and a grass mixture]. | 1972 | 4551838 | |
| critical evaluation of an indirect enzyme-linked immunosorbent assay for igg antibodies to dactylis glomerata. | experiments are reported for the evaluation of an indirect elisa using peroxidase conjugate for the detection of igg antibodies to dactylis glomerata (dg), a grass pollen antigen frequently responsible for allergic rhinitis and asthma. when optimized, the test proved to be useful in clinical practice for a semiquantitative, cheap and simply-performed determination of igg antibodies to dg. the advantages and disadvantages of this method, compared with those of elaborate antigen-binding assays, ar ... | 2007 | 4081522 |
| induction of a passive cutaneous anaphylaxis inhibitory factor in mice. | the administration of a purified protein from dactylis glomerata pollen, named dactylis inhibitory protein (dip), to balb/c mice results in the production of a heat-resistant seric factor that blocks the passive cutaneous anaphylaxis (pca) reaction in rat skin. this pca inhibitory factor (pca-if) was purified following different purification procedures and analyzed in sodium dodecyl sulfate polyacrylamide gel. amongst the array of bands detected it was possible to ascribe the inhibitory activity ... | 1985 | 4055080 |
| comparison of the stability of a mite and a pollen extract stored in normal conditions of use. | pollen extracts are stable in solutions containing 50% glycerol. the stability of dermatophagoides pteronyssinus (dp) extracts have not been determined. two lyophilised extracts (dp and cocksfoot pollen) were reconstituted in 50% glycerol and stored for up to 6 months at +4 degrees c. every day the extracts were placed at room temperature for 1 hr to mimic the natural use of allergen extracts by allergists. every 2 months the extracts initially prepared were compared to freshly reconstituted ext ... | 1985 | 3978775 |
| comparison between rush immunotherapy with a standardized allergen and an alum adjuved pyridine extracted material in grass pollen allergy. | rush immunotherapy with a standardized and lyophilized cocksfoot pollen extract was performed in twenty-three allergic subjects and compared with classical immunotherapy done with an alum adjuved, pyridine extracted cocksfoot pollen extract (fifteen subjects) and a control group of ten patients. the three groups were perfectly matched. clinical benefits were analysed by means of symptom scores. it was shown that rush immunotherapy gave significantly better results when asthma or rhinitis was con ... | 1985 | 3888440 |
| rast inhibition: a collaborative study on its suitability and reproducibility for the assay of cocksfoot pollen extracts. | the suitability and reproducibility of the inhibition mode of the radioallergosorbent test (rast) as a method for the assay of allergen extracts was examined in a collaborative study involving seven laboratories. participants assayed five coded extracts of cocksfoot grass (dactylis glomerata) pollen, including three batches from one manufacturer, both by a common method and by their local methods of rast inhibition. for the versions of rast inhibition employed in this study the estimates of rela ... | 2013 | 3719934 |
| preliminary experimental and clinical results with inactivated allergens conjugated to the corynebacterium granulosum-derived immunomodulator p40. | allergoids have been used successfully for immunotherapy of allergic disorders. it has appeared to us that the effect of allergoids could be potentiated by their coupling to an immunomodulator. in the present study we show that a conjugate made up of the coupling of ovalbumin through glutaraldehyde action to the c. granulosum-derived immunomodulator p40 is completely devoid of antigenicity and of cross-reactivity with ovalbumin. this conjugate was found to significantly inhibit mast cell degranu ... | 1987 | 3620123 |
| a comparison of two grass pollen extracts in the treatment of hay fever. | this trial of hyposensitisation compares the efficacy and safety of an alum absorbed 2-grass extract (phleum pratense and dactylis glomerata) with a traditional pyridine extracted alum precipitated 5-grass preparation whose efficacy has been demonstrated previously. 33 patients, (20 alpare, 13 allpyral), were entered into the trial which continued for 3 years; in the second year there were 17 alpare and 11 allpyral patients, and 13 alpare and 10 allpyral in the third year. patients on alpare req ... | 2006 | 3618442 |
| [comparative study of various technics for measuring serum ige levels]. | in this work we report the results of a comparative study of different techniques to measure specific ige in sera. three methods were used, phadebas rast, phadezym rast (pharmacia) and allergen-specific fast+ test (3m diagnostics): 127 sera were tested with several pneumoallergens (housedust, dermatophagoides pteronyssinus, dactylis glomerata pollen, cat and dog hair). analysis of the results shows the following correlations: phadebas and phadezym rast 92%; phadebas rast and fast+ 88%; phadezym ... | 1987 | 3453730 |
| utilization of magnesium and other macrominerals in sheep supplemented with different readily-fermentable carbohydrates. | sheep were used to determine the effects of dietary supplementation with readily-fermentable carbohydrates on mg, ca, k and p utilization. in each of two metabolism trials, 15 mature, crossbred wethers (average weight, 49.2 kg) were allotted to five dietary treatments consisting of 800 g/d of orchardgrass (dactylis glomerata, l.) hay alone, or supplemented with 450 g/d of either glucose, sucrose, lactose or starch. each trial consisted of a 5-d adjustment period, a 10-d preliminary period and a ... | 1987 | 3443586 |
| effect of ruminal infusion of glucose, volatile fatty acids and hydrochloric acid on mineral metabolism in sheep. | two experiments were conducted to study the effects of alterations in ruminal ph and volatile fatty acid (vfa) concentrations on utilization of mg and other minerals. in exp. 1, two metabolism trials were conducted with 12 ruminally cannulated crossbred wethers fed 800 g/d of orchard-grass (dactylis glomerata, l.) hay. after each feeding, wethers were ruminally infused with 500 ml (4.2 ml/min) or either 1) deionized water, 2) 40% (w/v) glucose solution, 3) .26 m propionic and .17 m butyric acid ... | 1988 | 3372390 |
| [identification of orchard grass (dactylis glomerata) pollen allergens by immunoblotting]. | 1988 | 3228359 | |
| selection among nonlinear models for rate of passage studies in ruminants. | eight toggenburg wether goats were fed either coastal bermudagrass (cynodon dactylon) or orchardgrass (dactylis glomerata)-alfalfa (medicago sativa) hay (57:43%) in a reversal design. four markers were used to study the movement of different digesta fractions: erbium for large particles greater than 1,700 microns; ytterbium for medium particles less than 1,700 microns and greater than 500 microns; chromium for small particles less than 500 microns and greater than 32 microns; and cobalt to follo ... | 1988 | 3225248 |
| characterization with monoclonal and polyclonal antibodies of a new major allergen from grass pollen in the group i molecular weight range. | we have purified a 24/25 kd allergen from orchard grass pollen (dactylis glomerata) that has an allergenic potency similar to that of the major group i allergen. we provisionally named this allergen grass 4b1 after the monoclonal antibody used for its identification and purification. this monoclonal antibody was obtained by immunizing mice with whole lolium perenne-pollen extract and by screening the antibody producing hybrids for reactivity with dactylis glomerata-pollen extract. grass 4b1 is p ... | 1989 | 2913133 |
| cloning of cdna coding for an allergen of cocksfoot grass (dactylis glomerata) pollen. | messenger rna isolated from cocksfoot grass (dactylis glomerata) anthers has been used to generate a cdna library in lambda t11. three cdna clones (7.8, 8.1, and 8.3) were demonstrated to be recognized by human ige antibodies in atopic serum and by rabbit polyclonal antiserum raised to a crude aqueous extract of cocksfoot pollen. the size of the cdna inserts was determined as approximately 700 bp, and restriction mapping demonstrated them to be identical sequences. lysogens obtained in escherich ... | 1989 | 2807580 |
| characterization of grass pollen-specific ige, iga, igm classes and igg subclasses in allergic patients. | water-soluble grass pollen extracts (dactylis glomerata) were separated by isoelectric focusing in a wide ph range (2-11) in agarose gel. after focusing, two successive gel prints were taken. the first one, on an ordinary nitrocellulose filter during 10 s, enabled the visualization of separated components of the pollen, after india ink staining. the second one, on a cyanogen bromide-activated nitrocellulose filter obtained after a 10-min transfer and saturation step, was incubated overnight with ... | 1989 | 2759718 |