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aerobic stability of wheat and orchardgrass round-bale silages during winter.using recently developed technology, balage is often stored in large (1.2 x 1.2 m) round bales that are wrapped in plastic film with an in-line wrapper. the aerobic stability of this fermented forage is important, particularly during winter months when it is fed to livestock or sold as a cash crop. two types of forage, orchardgrass [dactylis glomerata l.; 54.4% dry matter (dm)] and wheat (triticum aestivum l.; 62.4% dm), were packaged in large round bales and wrapped with an in-line wrapper duri ...200515829675
factors affecting translation at the programmed -1 ribosomal frameshifting site of cocksfoot mottle virus rna in vivo.the ratio between proteins p27 and replicase of cocksfoot mottle virus (cfmv) is regulated via a -1 programmed ribosomal frameshift (-1 prf). a minimal frameshift signal with a slippery u uua aac heptamer and a downstream stem-loop structure was inserted into a dual reporter vector and directed -1 prf with an efficiency of 14.4 +/- 1.9% in yeast and 2.4 +/- 0.7% in bacteria. p27-encoding cfmv sequence flanking the minimal frameshift signal caused approximately 2-fold increase in the -1 prf effic ...200515843686
immunotherapy with depigmented glutaraldehyde-polymerized extracts: changes in quality of life.immunotherapy (it) with modified allergens reduces allergic rhinitis (ar) symptoms and medications requirements. improvement of quality of life (qol) is a key point in the treatment of ar. the aim of this study was to provide evidence of changes related to the patient's qol (well-being) induced by a modified (depigmented glutaraldehyde-polymerized) therapeutic vaccine and of its safety.200515898977
orchard grass or cock's foot, dactylis glomerata. 200515945550
[sensitization to castanea sativa pollen and pollinosis in northern extremadura (spain)].castanea sativa pollen allergy has generally been considered to be uncommon and clinically insignificant. in our geographical area (plasencia, cáceres, spain) castanea sativa pollen is a major pollen.200515946627
bioaccumulation of heavy metals in dactylis glomerata l. growing in a calcareous soil amended with sewage sludge.the total and dtpa-extractable concentrations of pb, ni, zn, cu, cr and cd were measured in a calcareous soil amended with different doses of sewage sludge under field conditions. the same metals were also measured in the roots and leaves of dactylis glomerata at the end of the first vegetative period after the sludge was added. the root concentrations of all the metals were unrelated to their concentrations in the soil. leaf concentrations of zn and cr correlated with total (zn) and dtpa-extrac ...200615953718
surfactant protein d decreases pollen-induced ige-dependent mast cell degranulation.mast cells play a key role in allergy and asthma. they reside at the host-environment interface and are among the first cells to make contact with inhaled microorganisms and particulate antigens. pulmonary surfactant proteins a and d (sp-a and sp-d) function in lung host defense by enhancing microbe phagocytosis and mediating other immune cell functions, but little is known about their effects on mast cells. we hypothesized that sp-a and/or sp-d modulate ige-dependent mast cell functions. pollen ...200515980037
[the frequency of pollen allergy at the population of sarajevo region during the 2002 year].the frequency of pollen alergy in the sample of population of sarajevo region during the 2002 year has been investigated. the age and gender population has been different. for determination of allergy has been use of standard test carryed out in the respective laboratories of clinical center of university of sarajevo. the testing has been on the pollen of weed plant species and grass (graminejae) pollen. in the mixture of pollen weed plant species have been following plants: plantago lanceolata ...200516018386
limited genetic exchanges between populations of an insect pest living on uncultivated and related cultivated host plants.habitats in agroecosystems are ephemeral, and are characterized by frequent disturbances forcing pest species to successively colonize various hosts belonging either to the cultivated or to the uncultivated part of the agricultural landscape. the role of wild habitats as reservoirs or refuges for the aphid sitobion avenae that colonize cultivated fields was assessed by investigating the genetic structure of populations collected on both cereal crops (wheat, barley and oat) and uncultivated hosts ...200516024367
monoclonal antibody against a cell wall marker protein for embryogenic potential of dactylis glomerata l. suspension cultures.we identified and isolated a monoclonal antibody (mab 3g2) raised against extracellular proteins from microcluster cells of orchard grass (dactylis glomerata l.) embryogenic suspension culture. mab 3g2 recognized with high specificity an antigen ionically bound within the primary cell wall and in the culture medium of microcluster cells. two-dimensional polyacrylamide gel analysis and blotting of proteins on pvdf membrane showed that mab 3g2 detected a single polypeptide of apparent molecular ma ...200516025341
a cd14 gene polymorphism is associated with the ige level for dermatophagoides pteronyssinus.cd14/-159 plays a role in sensitization to dermatophagoides pteronyssinus in japanese patients with allergic rhinitis.200516109674
herbaceous vegetation productivity, persistence, and metals uptake on a biosolids-amended mine soil.the selection of plant species is critical for the successful establishment and long-term maintenance of vegetation on reclaimed surface mined soils. a study was conducted to assess the capability of 16 forage grass and legume species in monocultures and mixes to establish and thrive on a reclaimed appalachian surface mine amended with biosolids. the 0.15-ha coarse-textured, rocky, non-acid forming mined site was prepared for planting by grading to a 2% slope and amending sandstone overburden ma ...201216151233
ruminal in situ disappearance kinetics of nitrogen and neutral detergent insoluble nitrogen from common crabgrass forages sampled on seven dates in northern arkansas.southern crabgrass (digitaria ciliaris [retz.] koel.) is often an undesirable species in field and forage crops, but visual observations suggest that livestock prefer it to many other summer forages. the objectives of this study were to assess the nutritive value of crabgrass sampled weekly between july 11 and august 22, 2001 and then to determine ruminal in situ disappearance kinetics of n and neutral detergent insoluble n (ndin) for these forages. a secondary objective was to compare these kin ...200616478959
effect of manure application timing, crop, and soil type on nitrate leaching.timing of manure application affects n leaching. this 3-yr study quantified n losses from liquid manure application on two soils, a muskellunge clay loam and a stafford loamy sand, as affected by cropping system and timing of application. dairy manure was applied at an annual rate of 93 800 l ha(-1) on replicated drained plots under continuous maize (zea mays l.) in early fall, late fall, early spring, and as a split application in early and late spring. variable rates of supplemental sidedress ...200616510712
surveys of new and rare microfungi in the düsseltal (north rhine-westphalia)--germany.in the years 2003 and 2004 we have observed an about 70 hectare large area in the düsseltal, the eastern part of the neandertal in north rhine-westphalia. there we collected on trees, bushes and herbs and found about 150 microfungi of which some are new for germany or the entire world. e.g.: pseudocercospora populigena n. ale-agha, u. braun & g.b. feige on populus berolinensis; vialaea insculpta (fr.) sacc. on ilex aquifolium l.; passalora amelopsidis (peck.) u. braun on parthenocissus quinquefo ...200516637187
aerating grassland before manure application reduces runoff nutrient loads in a high rainfall environment.the effect of mechanically aerating grassland before liquid manure application in the fall on surface runoff and transport of nutrients and solids was studied in a high rainfall area. the two treatments were control and aeration, the latter receiving one pass with an aerator perpendicular to the slope before fall application of liquid manure (dairy in years 1-3 and swine in year 4). treatments were randomly assigned on 3 to 5% sloping land with a silt loam surface soil (aquic dystroxerept) plant ...200616641328
partition of membrane particles in aqueous two-polymer phase system and its practical use for purification of plasma membranes from plants.a simplified method for the isolation of a plasma membrane-enriched fraction from plants utilizing an aqueous two-polymer phase system is outlined. mainly, the plant used was orchard grass (dactylis glomerata l.). the two-phase system consisted of 5.6% (w/w) of dextran t500 and 5.6% (w/w) of polyethyleneglycol 4000 in 0.5 molar sorbitol-15 millimolar tris-maleate (ph 7.3), and 30 millimolar nacl. in this system, the plasma membranes and the other membranes were preferentially partitioned into th ...198316662942
protein and lipid compositions of isolated plasma membranes from orchard grass (dactylis glomerata l.) and changes during cold acclimation.the chemical composition of plasma membrane fractions isolated from orchard grass seedlings (dactylis glomerata l.) was analyzed and compared with endomembranes. the plasma membrane is characterized by an enrichment of sterols and a lower degree of unsaturation of phospholipids. steryl glycosides were found to be one of the lipid components of the plasma membrane, but steryl esters and galactolipids were barely detectable. diphosphatidyl glycerol was characteristically detected in the mitochondr ...198416663596
studies on freezing injury of plant cells: i. relation between thermotropic properties of isolated plasma membrane vesicles and freezing injury.the thermotropic transition of plasma membrane of dactylis glomerata was studied by using fluorescence polarization of embedded fluorophore, 1,6-diphenyl-1,3,5-hexatriene. under the presence of 35% ethylene glycol, reversible thermotropic transitions were observed in isolated plasma membrane vesicles in nearly the same temperature range as the temperature of freezing injury to cells. in liposomes prepared from isolated plasma membranes, however, the thermotropic transitions occurred at much lowe ...198416663597
partial purification and properties of phleinase induced in stem base of orchardgrass after defoliation.phleinase induced in stem base of orchardgrass (dactylis glomerata l.) after defoliation was partially purified with ammonium sulfate precipitation, deae-sephadex chromatography, gel filtration, and preparative polyacrylamide gel electrophoresis. the molecular weight of phleinase was 57,000 as determined by gel chromatography. the enzyme showed normal michaelis-menten kinetics and its k(m) value was 91 millimolar for phlein of mean degree of polymerization 60 as substrate. reaction velocity of t ...198516664288
inhibition of somatic embryogenesis in orchardgrass by endogenous cytokinins.endogenous indoleacetic acid (iaa) and cytokinin concentrations were measured by high performance liquid chromatography in leaf sections of an orchardgrass (dactylis glomerata l.) genotype which exhibited a high capacity for somatic embryogenesis in vitro and in two genotypes that did not exhibit this capacity. the nonembryogenic genotypes contained 3- to 4-fold higher concentrations of zeatin, zeatin riboside, dihydrozeatin, dihydrozeatin riboside, and total cytokinins than the embryogenic geno ...198816666493
detection of kestoses and kestose-related oligosaccharides in extracts of festuca arundinacea, dactylis glomerata l., and asparagus officinalis l. root cultures and invertase by c and h nuclear magnetic resonance spectroscopy.a previous study (kl forsythe, ms feather [1989] carbohydr res 185: 315-319) showed that (13)c nuclear magnetic resonance spectroscopy can be used to detect and identify mixtures of 1-kestose and neokestose after conversion to the acetate derivatives. in this study, unequivocal assignments are made for the anomeric carbon and proton signals for the above two trisaccharide acetates as well as for 6-kestose hendecaacetate and for nystose tetradecaacetate (a 1-kestose-derived tetrasaccharide). a nu ...199016667365
effects of management regime and plant species on the enzyme activity and genetic structure of n-fixing, denitrifying and nitrifying bacterial communities in grassland soils.management by combined grazing and mowing events is commonly used in grasslands, which influences the activity and composition of soil bacterial communities. whether observed effects are mediated by management-induced disturbances, or indirectly by changes in the identity of major plant species, is still unknown. to address this issue, we quantified substrate-induced respiration (sir), and the nitrification, denitrification and free-living n(2)-fixation enzyme activities below grass tufts of thr ...200616689721
changes in terpene content in milk from pasture-fed cows.changes of terpene content in milk from cows grazing natural diversified upland pasture were examined in this observational trial. a homogeneous plot divided into 2 subplots was used from may 31 to july 1, 2003 (first growth) and again from october 1 to october 7, 2003 (vegetative regrowth). each subplot was grazed by 6 dairy cows in 2 ways: strip grazing (sg), with new allocations of pasture strips at 2-d intervals, and paddock grazing (pg). the pg subplot was divided into 3 paddocks and the co ...200616702298
p1 protein of cocksfoot mottle virus is indispensable for the systemic spread of the virus.cocksfoot mottle sobemovirus (cfmv) encodes a non-conserved protein p1 from the 5' orf1 of genomic rna. the functions of cfmv p1 are unknown. in the current study we show that p1-deficient cfmv can replicate both in oat leaves and barley suspension culture cells but can not infect oat plants systemically. however, the absence of p1 reduces the efficiency of virus accumulation considerably. the infectivity of the mutant virus restores as a result of the spontaneous transversion. cfmv p1:egfp show ...200616732485
the pigments of butterflies' wings: occurrence of the pigment of melanargia galatea in dactylis glomerata. 192616743736
the wax constituents of forage grasses: cocksfoot and perennial ryegrass. 193116744781
the glyceride fatty acids of forage grasses: cocksfoot and perennial ryegrass. 193216744806
the phosphatides of forage grasses: cocksfoot. 193216744943
the isolation of carotene and sterols from the unsaponifiable matter of cocksfoot. 193616746034
the composition of forage crops: cocksfoot. changes in the herbage during growth, with and without additions of nitrogenous fertilizer. 193916747022
fractional and structural characterization of hemicelluloses from perennial ryegrass (lolium perenne) and cocksfoot grass (dactylis glomerata).sequential three-stage treatments with 80% etoh containing 0.2% naoh, 2.5% h2o2-0.2% edta containing 1.5% naoh and 2.5% h2o2-0.2% taed containing 1.0% naoh at 75 degrees c for 3h released 8.0% and 10.4%, 79.1% and 77.0% and 12.9% and 12.5% of the original hemicelluloses from perennial grass and cocksfoot grass, respectively. it was found that the four alkaline peroxide-soluble hemicellulosic fractions contained higher amounts of xylose (33.4-38.2%), uronic acids (9.3-15.3%) and rhamnose (3.0-3.9 ...200616750181
[aflp analysis on genetic diversity of wild dactylis glomerata l. germplasm resources].thirty-seven accessions of wild dactylis glomerata l. were used in assessment of genetic diversity by means of amplified fragment length polymorphism (aflp). nine primer pairs were selected from 64 preparations and then used to analyze the plant materials. a total of 400 dna fragments were amplified, among which 336 (84.0%) were polymorphic. genetic distance, from 0.0692-0.4214 among accessions, was calculated by the ntsys-pc software. a dendrogram was constructed using upgma based on genetic di ...200616825173
role of rainfall intensity and hydrology in nutrient transport via surface runoff.loss of soil nutrients in runoff accelerates eutrophication of surface waters. this study evaluated p and n in surface runoff in relation to rainfall intensity and hydrology for two soils along a single hillslope. experiments were initiated on 1- by 2-m plots at foot-slope (6%) and mid-slope (30%) positions within an alfalfa (medicago sativa l.)-orchardgrass (dactylis glomerata l.) field. rain simulations (2.9 and 7.0 cm h(-1)) were conducted under wet (spring) and dry (late-summer) conditions. ...201316825444
seed germination in response to chemicals: effect of nitrogen and ph in the media.seed germination generally presents a peak in the next growing season after a fire. among other factors associated with fire are the increase of soil nitrogen and changes in the ph of the soil. in this study, we addressed the question, whether or not the germination response of eight species is linked with the increase in ph and nitrogenous compounds in the germination media? we assessed the separate and combined effects of nitrogenous compounds and ph on the percentage and rate of germination o ...200616850869
[genetic diversity of dactylis glomerata germplasm resources detected by inter-simple sequence repeats (issrs) molecular markers].inter-simple sequence repeat (issr) molecular markers were used to detect the genetic diversity among 50 materials of dactylis glomerata collected from china and other countries. twelve primers produced 101 polymorphic bands, averaged 8.41 bands each primer pair. the average percentage of polymorpgic bands was 86.3.8%, and the range of gs (define) was 0.6116-0.9290, indicating a rich genetic diversity of d. glomerata. based on the cluster and principal component analyses on the genetic character ...200616963418
cocksfoot mottle virus p1 suppresses rna silencing in nicotiana benthamiana and nicotiana tabacum.the sobemovirus genome consists of positive sense, single-stranded polycistronic rna. the 5'-terminal orf, encoding the protein p1, is its most variable region. sobemoviral p1 has been described as dispensable for replication but indispensable for systemic infection. the p1 of rice yellow mottle virus-nigerian isolate (rymv-n) is the only rna silencing suppressor reported for sobemoviruses until now. using an agrobacterium-mediated transient assay, we demonstrate here that p1 of cocksfoot mottle ...200716971015
an attempt to identify recombinants between two sobemoviruses in doubly infected oat plants.recombination in rna viruses is considered to play a major role as a driving force in virus variability to counterbalance loss in fitness that can be due to the accumulation of detrimental mutations. studies on mixed infections are pertinent for understanding the role of recombination in virus evolution. they also provide important baseline information for studying the biosafety of plants expressing viral sequences. to investigate the possibility of rna recombination occurrence between two sobem ...200616978574
nitrogen- vs. phosphorus-based dairy manure applications to field crops: nitrate and phosphorus leaching and soil phosphorus accumulation.management of animal manures to provide nutrients for crop growth has generally been based on crop n needs. however, because manures have a lower n/p ratio than most harvested crops, n-based manure management often oversupplies the crop-soil system with p, which can be lost into the environment and contribute to eutrophication of water bodies. we examined the effects of n- vs. p-based manure applications on n and p uptake by alfalfa (medicago sativa l.), corn (zea mays l.) for silage, and orchar ...201317071901
sobemoviruses possess a common cfmv-like genomic organization.based on structural differences in the orf2 region, the sobemoviruses have been subdivided into southern cowpea mosaic virus (scpmv)-like and cocksfoot mottle virus (cfmv)-like types of genome organization. however, nearly identical amino acid sequences are encoded by these subgroups in different reading frames of orf2, suggesting that insertion or deletion of appropriate nucleotides could restore similar genomic organizations for these viruses. we resequenced the regions of inconsistency for is ...200717115301
increased meiotic irregularity accompanying inbreeding in dactylis glomerata l. 194317247095
the ryegrass mottle virus genome codes for a sobemovirus 3c-like serine protease and rna-dependent rna polymerase translated via -1 ribosomal frameshifting.in the course of sobemovirus gene cloning the complete genome of ryegrass mottle virus (rgmov) was sequenced. sequence analysis revealed differences including missing and extraneous nucleotides in comparison to the previously published sequence (zhang, toriyama, takanashi, j. gen. plant pathol. 67, 63 (2001)). a gene coding for a typical sobemovirus 3c-like serine protease was identified in orf2a after multiple sequence alignment analysis. the newly identified 57-amino-acid stretch in orf2a show ...200717356908
simulation of germination of pioneer species along an experimental drought gradient.the germination of ten plant species from the iberian peninsula was assessed along a water deficit gradient between -0. 1652 (moist) and -0.4988 mpa (dry) of osmotic potential, created by addition of increasing concentrations of polyethylene glycol (peg 6000) to distilled water in which plants were grown hydroponically. the level and rate of germination of daucus carota and thapsia villosa significantly decreased with decreasing psi. seeds of dactylis glomerata and dittrichia viscosa had positiv ...200617405330
direct embryogenesis from mesophyll cells of orchardgrass.segments taken from the basal 15 to 20 millimeters of the two innermost leaves of an orchardgrass (dactylis glomerata l.) genotype produced somatic (nonzygotic) embryos directly from mesophyll cells without an intervening callus when cultured on an agar medium with 30 micromolar 3,6-dichloro-o-anisic acid (dicamba). this demonstration of high-frequency embryogenesis from mesophyll cells in gramineae is strong evidence for totipotency of the cells.198317753478
unraveling the effects of management regime and plant species on soil organic carbon and microbial phospholipid fatty acid profiles in grassland soils.in a field experiment we have examined the effect of long-term grassland management regimes (viz., intensive versus extensive) and dominant plant species (viz., arrhenatherum elatius, holcus lanatus and dactylis glomerata) on soil organic carbon (soc) build up, soil microbial communities using biomarker phospholipid fatty acids (plfa), and the relationship between soc and plfas of major groups of microorganisms (viz., bacteria, fungi, and actinomycetes). the results have revealed that changes in ...200817826981
effect of intake level and alfalfa substitution for grass hay on ruminal kinetics of fiber digestion and particle passage in beef cattle.two experiments were conducted to evaluate digestion kinetics of alfalfa (medicago sativa l.) substitution for grass hay in beef cattle. in exp. 1, forage combinations evaluated in situ consisted of 0% alfalfa-100% big bluestem (andropogon gerardi vitman), 25% alfalfa-75% big bluestem, 50% alfalfa-50% big bluestem, and 100% alfalfa-0% big bluestem. nonlinear regression was used to determine the immediately soluble fraction a, the potentially degradable fraction b, the undegraded fraction c, and ...200817940159
effective rumen degradation of dry matter, crude protein and neutral detergent fibre in forage determined by near infrared reflectance spectroscopy.the objective of the present study was to examine if near infrared reflectance spectroscopy (nirs) could be used to predict degradation parameters and effective degradation from scans of original forage samples. degradability of dry matter (dm), crude protein (cp) and neutral detergent fibre (ndf) of 61 samples of perennial ryegrass (lolium perenne l.) and orchardgrass (dactylis glomerata l.) was tested by using the in situ technique. the grass samples were harvested at three different stages, e ...200717988354
antigenic similarity among group 1 allergens from grasses and quantitation elisa using monoclonal antibodies to phl p 1.group 1 allergens elicit a specific ige response in about 90% of grass pollen-allergic patients. the aim of this work was to study the antigenic similarity among group 1 allergens from different grasses and to develop a monoclonal antibody (mab)-based quantitation elisa.200818004069
retrospective study of specific immunotherapy - what should be done in the future.in this retrospective study, data on 241 atopic patients treated with specific cutaneous immunotherapy during the 1985-2006 period at allergy clinic, university department of dermatology and venereology, were reviewed. the following diagnoses were recorded: atopic dermatitis, pure or in combination with allergic rhinitis or allergic bronchitis, or allergic bronchitis and asthma, allergic rhinitis, allergic conjunctivitis, urticaria, and quincke's edema. the aim was to retrospectively analyze cli ...200718093449
phenotypes and functional effects caused by various viral rna silencing suppressors in transgenic nicotiana benthamiana and n. tabacum.rna silencing suppressor genes derived from six virus genera were transformed into nicotiana benthamiana and n. tabacum plants. these suppressors were p1 of rice yellow mottle virus (rymv), p1 of cocksfoot mottle virus, p19 of tomato bushy stunt virus, p25 of potato virus x, hcpro of potato virus y (strain n), 2b of cucumber mosaic virus (strain kin), and ac2 of african cassava mosaic virus (acmv). hcpro caused the most severe phenotypes in both nicotiana spp. ac2 also produced severe effects in ...200818184062
evidence for gc preference by monocot dicer-like proteins.dicot dicer-like (dcl) enzymes operate preferably on gc rich regions when producing small interfering (si)rna and micro (mi)rna. this gc bias, however, is not generic in monocot mirna productions. from wild dactylis glomerata naturally infected by cocksfoot streak potyvirus (csv), csv-sirnas had a greater gc% than the virus genome, indicating that gc rich regions were also preferred by the grass dcls. this supports the notion that gc preference is an ancient feature for plant dcls, and suggests ...200818243131
specific immunotherapy for common grass pollen allergies: pertinence of a five grass pollen vaccine.patients throughout europe are concomitantly exposed to multiple pollens from distinct pooideae species. given the overlap in pollination calendars and similar grain morphology, it is not possible to identify which grass species are present in the environment from pollen counts. furthermore, neither serum ige reactivity nor skin prick testing allow the identification of which grass species are involved in patient sensitisation. due to their high level of amino acid sequence homology (e.g., >90% ...200818362477
forage systems for cow-calf production in the appalachian region.small cow-calf operations are common in the appalachian region. tall fescue [lolium arundinaceum (schreb.) s. j. darbyshire] is the dominant forage in these systems for direct grazing as well as for stockpiling. the present study was conducted from 2001 to 2005. a total of 108 angus and angus crossbred cows were allotted randomly to 6 forage systems and then to 3 replicates within each system. in brief, system 1 had a stocking rate of 0.91 ha/cow in a middleburg 3-paddock (a, b, and c) system. s ...200818407993
cluster immunotherapy with a glutaraldehyde-modified mixture of grasses results in an improvement in specific nasal provocation tests in less than 2.5 months of treatment.cluster immunotherapy is becoming increasingly used. it allows for a rapid build up phase and the administration of higher doses of allergen in a shorter period of time.200818445082
double-blind, placebo-controlled evaluation of grass pollen specific immunotherapy with oral drops administered sublingually or supralingually.forty-one patients suffering from grass pollen allergy underwent specific immunotherapy with standardized allergen extract consisting of six grass pollens (h-al per os) administered either sublingually or supralingually for one year. in order to investigate clinical and immunological changes induced by the administration of allergens via the oral mucosa, the double-blind, placebo-controlled, randomized design of the trial with 30 other patients enrolled in placebo groups was applied. specific im ...200818585908
generation of recombinant antibodies against orchardgrass acidic nsltp-like proteins.embryogenic and non-embryogenic suspension cultures of orchardgrass (dactylis glomerata l.) secreted into the culture medium a set of proteins, among which low molecular mass (11/12 kda) proteins were found. however, only the 11/12 kda proteins from the embryogenic suspension cultures reacted specifically with an antiserum raised against the carrot ep2 non-specific lipid transfer protein (nsltp). two-dimensional (2-d) electrophoretic analysis revealed that the extracellular nsltp-like proteins f ...200918669026
biological and molecular characterization of a putative new sobemovirus infecting imperata cylindrica and maize in africa.a new virus was isolated from both the grass imperata cylindrica and maize plants that had yellow mottle symptoms in burkina faso, west africa. the virus has isometric particles ca. 32 nm in diameter. the experimental host range was restricted to rottboellia exaltata. virions were isolated from leaves of systemically infected maize plants. koch's postulates were completed by mechanically inoculating uninfected imperata or maize with either purified virus or sap from infected imperata plants. vir ...200818777157
studies on the origin of dactylis glomerata l. 194818903868
extraction, structural characterisation and evaluation of hydroxycinnamate esters of orchard grass (dactylis glomerata) as substrates for polyphenol oxidase.polyphenol oxidase (ppo) activity has been reported in orchard grass (dactylis glomerata); however, to date, no endogenous substrates have been identified. in the present study, we report the isolation and structural elucidation of ppo substrates in this species. the free phenol fraction was extracted, separated by reverse-phase chromatography and six potential substrates, including two hydroxycinnamate esters, were identified by uv spectrometry, electrospray ionisation-tandem mass spectrometry ...200818929376
uptake, transport, re-translocation and chelation of ni in one tolerant and one susceptible graminaceous species.from flora of northwest iran, two graminaceous species including secale montanum and dactylis glomerata with different distribution and coverage on ni-rich soils were selected for study of tolerance mechanisms to ni concentrations up to 100 microm in hydroponic culture medium. shoot and root growth of d. glomerata was inhibited up to 42 and 69% in response to 100 microm ni, respectively, while growth reduction of s. montanum was as low as 20% in shoot and no growth inhibition of root was observe ...200719070044
[physiological bases of herbages shade-tolerance in eucalyptus grandis and herbage inter-cultivated system].the study on the physiological bases of the shade-tolerance of dactylis glomerata, lolium perenne, and hemarthria compressa inter-cultivated in eucalyptus grandis forests with different canopy densities showed that with increasing canopy density, the light saturation point (lsp), light compensation point (lcp), chlorophyll a/b (chla/chlb), and the contents of proline (pro), soluble sugar (ss) and soluble protein (sp) of the three herbages decreased gradually, but the apparent quantum yield (aqy) ...200819102302
biodiversity effects on yield and unsown species invasion in a temperate forage ecosystem.current agricultural practices are based on growing monocultures or binary mixtures over large areas, with a resultant impoverishing effect on biodiversity at several trophic levels. the effects of increasing the biodiversity of a sward mixture on dry matter yield and unsown species invasion were studied.200919168861
biogas production from boreal herbaceous grasses--specific methane yield and methane yield per hectare.the objective of this study was to determine the specific methane yields of four grass species (cocksfoot, tall fescue, reed canary grass and timothy) cultivated under boreal conditions as well as how harvesting time and year of cultivation affects the specific methane yields per ha. the specific methane yields of all grasses and all harvests varied from 253 to 394 nl ch4/kg volatile solids (vs) added. the average specific methane yield of the 1st harvest of all grasses was higher than the 2nd h ...200919261471
identification of seed gall nematodes of agronomic and regulatory concern with pcr-rflp of its1.a molecular analysis of eight described species of seed gall nematode, along with six undescribed isolates from different hosts, has revealed a strong association between nucleotide sequence polymorphism and host status. each anguinid nematode associated with a unique host produced a unique pcr-rflp pattern for the its1 region. anguina species that had been synonymized in the past, anguina agrostis, a. funesta, and a. wevelli (afrina wevelli), were readily discriminated. two undescribed species ...200119265880
a comparative analysis of extraction methods for the recovery of anguina sp. from grass seed samples.four procedures were compared in their efficacy to extract juveniles of anguina agrostis from commercial grass seed. the procedures included those currently used by the state regulatory laboratories of oregon and california, as well as new tests developed to determine juvenile viability for the phytosanitary certification of fumigated grass seed. eleven seed lots of agrostis tenuis (bentgrass) and dactylis glomerata (orchardgrass) naturally infested with varying levels of juveniles of anguina we ...199919270928
additional hosts for the ring nematode, criconemella xenoplax.some common legumes and weeds indigenous to peach orchards in south carolina were tested in greenhouse experiments to determine their host suitability for criconemella xenoplax. legumes that were hosts for the nematode were dwarf english trefoil (lotus corniculatus var. arvensis), big trefoil (l. uliginosis), birdsfoot trefoil (l. corniculatus), narrowleaf birdsfoot trefoil (l. tenuis), ball clover (trifolium nigrescens), rose clover (t. hirtum), subterranean clover (t. subterraneum), striate le ...199019287692
a leachate a day keeps the seedlings away: mowing and the inhibitory effects of festuca paniculata in subalpine grasslands.is the release of allelochemicals by the dominant tussock grass festuca paniculata responsible for its dominance by inhibiting growth of neighbour grasses in subalpine grasslands? as such a community is also structured by mowing practices, what could be the impact of mowing on allelopathy?200919324898
responses of plasma acetate metabolism to hop (humulus lupulus l.) in sheep.an isotope dilution method using [1-(13)c]sodium (na) acetate was conducted to determine the effect of feeding hop (humulus lupulus l.) residues on plasma acetate metabolism in six adult crossbred sheep. the sheep were fed 63 g/kg bw(0.75)/d of either mixed hay (mh-diet) of orchardgrass (dactylis glomerata l.) and reed canarygrass (phalaris arundinacea l.) at a 60:40 ratio or mh-diet and hop-residues (hop-diet) at 85:15 ratio with a crossover design for each of 3 week period. the isotope dilutio ...200919365576
water deficit and induction of summer dormancy in perennial mediterranean grasses.summer dormancy is a trait conferring superior drought survival in mediterranean perennial grasses. as the respective roles of environmental factors and water deficit on induction of summer dormancy are unclear, the effect of intense drought were tested under contrasting day lengths in a range of forage and native grasses.200919369219
effects of rumen fill on short-term ingestive behavior and circulating concentrations of ghrelin, insulin, and glucose of dairy cows foraging vegetative micro-swards.the objective of this study was to investigate changes in foraging behavior, hunger-related hormones, and metabolites of dairy cows in response to short-term variations in rumen fill (rf). the effect of rf on intake rate, jaw movements, bite rate and dimensions, and concentrations of plasma ghrelin, and serum insulin and glucose were measured in 4 rumen-cannulated lactating dairy cows (612 +/- 68 kg, empty live weight; 237 +/- 29 d in milk) foraging micro-swards of vegetative orchardgrass (dacty ...200919389967
effects of bale moisture and bale diameter on spontaneous heating, dry matter recovery, in vitro true digestibility, and in situ disappearance kinetics of alfalfa-orchardgrass hays.alfalfa (medicago sativa l.)-orchardgrass (dactylis glomerata l.) hay was made in 96 large-round bales over 3 harvests during 2006 and 2007 to assess the effects of spontaneous heating on dry matter (dm) recovery, in vitro true digestibility (ivtd), and in situ disappearance kinetics of dm. throughout these harvests, bales were made at preset diameters of 0.9, 1.2, or 1.5 m and at moisture concentrations ranging from 9.3 to 46.6%. internal bale temperatures were monitored daily during an outdoor ...200919448019
effects of spontaneous heating on fiber composition, fiber digestibility, and in situ disappearance kinetics of neutral detergent fiber for alfalfa-orchardgrass hays.during 2006 and 2007, forages from 3 individual hay harvests were utilized to assess the effects of spontaneous heating on concentrations of fiber components, 48-h neutral detergent fiber (ndf) digestibility (ndfd), and in situ disappearance kinetics of ndf for large-round bales of mixed alfalfa (medicago sativa l.) and orchardgrass (dactylis glomerata l.). over the 3 harvests, 96 large-round bales were made at preset bale diameters of 0.9, 1.2, or 1.5 m, and at moisture concentrations ranging f ...200919448020
safety and tolerability of seasonal ultra-rush, high-dose sublingual-swallow immunotherapy in allergic rhinitis to grass and tree pollens: an observational study in 193 children and adolescents.we conducted a large observational study in 193 children and adolescents with allergic rhinitis due to grass or tree pollens to evaluate the safety and tolerability of an ultrarush high-dose sublingual immunotherapy (slit) regimen reaching a maintenance dose of 300 index of reactivity within 90 minutes.200919476016
[genetic variation and relationship in orchardgrass (dactylis glomerata l.) germplasm detected by ssr markers].genetic variation and relationship of 53 accessions of d. glomerata collected from 5 continents were analyzed using simple sequence repeat (ssr) molecular markers with 15 ssr primer pairs. the following results were obtained. (1) a total of 127 alleles were detected at 15 loci. the number of alleles per locus ranged from 5 to 12, with an average of 8.5. the rate of polymorphic sites (p) was 95.21%; the polymorphic information content (pic) ranged from 0.30(a04c24)to 0.44(a01f24) with an average ...200919586867
fatty acid metabolism and deposition in subcutaneous adipose tissue of pasture- and feedlot-finished cattle.an experiment was conducted to evaluate the effects of pasture finishing versus feedlot finishing, over time, on fatty acid metabolism in angus crossbred steers (n = 24). ruminal fluid, serum, and adipose tissue biopsies were obtained on d 0, 28, 84, and 140. pasture forages and diet ingredient samples were obtained at 14-d intervals to determine nutritive value and fatty acid composition. the feedlot diet consisted of corn silage, cracked corn grain, soybean meal, and a vitamin and mineral supp ...200919617512
effect of supplementation of grazing dairy ewes with a cereal concentrate on animal performance and milk fatty acid profile.this work was conducted to investigate the effect of supplementing grazing ewes on pasture with a cereal concentrate on the milk fatty acid (fa) profile. ninety assaf ewes in mid lactation were distributed in 9 lots of 10 animals each and allocated to 3 feeding regimens: 1) pasture--ewes were only allowed to graze pasture (an irrigated sward of lolium perenne, trifolium pratense, and dactylis glomerata); 2) ps--grazing ewes were supplemented with oat grain (700 g/animal and day); and 3) tmr--ewe ...200919620680
comparative sequence analysis and serological and infectivity studies indicate that cocksfoot mild mosaic virus is a member of the genus panicovirus.the complete nucleotide sequence of the phleum isolate of cocksfoot mild mosaic virus (cmmv-p) and the coat protein sequence of the cocksfoot isolate (cmmv-1) were determined. comparative sequence analysis revealed a close relationship with panicum mosaic virus (pmv; genus panicovirus), and together with serological studies, the work supports the classification of cmmv in the family tombusviridae, genus panicovirus rather than, as is currently proposed, the genus sobemovirus. a full-length cdna ...200919693646
stem-loop structure of cocksfoot mottle virus rna is indispensable for programmed -1 ribosomal frameshifting.the -1 programmed ribosomal frameshifting (-1 prf) mechanism utilized by many viruses is dependent on a heptanucleotide slippery sequence and a downstream secondary structure element. in the current study, the rna structure downstream from the slippery site of cocksfoot mottle sobemovirus (cfmv) was proven to be a 12bp stem-loop with a single bulge and a tetranucleotide loop. several deletion and insertion mutants with altered stem-loop structures were tested in wheat germ extract (wge) for fram ...200919748532
rhizosphere microbial densities and trace metal tolerance of the nickel hyperaccumulator alyssum serpyllifolium subsp. lusitanicum.in this study we determine culturable microbial densities (total heterotrophs, ammonifiers, amylolytics and cellulolytics) and bacterial resistance to co, cr, and ni in bulk and rhizosphere soils of three populations of the ni-hyperaccumulator alyssum serpyllifolium subsp. lusitanicum and the excluder dactylis glomerata from ultramafic sites (two populations in northeast (ne) portugal (samil (s), morais (m)) and one population in northwest (nw) spain (melide (l)). the relationship between bioava ...200919810353
optimization of culture conditions of arnica montana l.: effects of mycorrhizal fungi and competing plants.arnica montana is a rare plant that needs special protection because of its intensive harvesting for medicinal purposes. the present work was aimed at finding optimal culture conditions for arnica plants in order to enable their successful reintroduction into their natural stands. plants were cultivated under controlled greenhouse conditions on substrata with different nitrogen (n) concentration. as arnica is always colonized by arbuscular mycorrhizal fungi (amf) in nature, a fact that has been ...201019838743
characterization of allergenic epitopes of ory s1 protein from oryza sativa and its homologs.vaccination is the most effective technique suggested now days for allergy treatment. recombinant-based approaches are mostly focused on genetic modification of allergens to produce molecules with reduced allergenic activity and conserved antigenicity. the molecules developed for vaccination in allergy possess significantly reduced allergenicity in terms of ige binding, and therefore will not lead to anaphylactic reactions upon injection. this approach is probably feasible with every peptide all ...200920011147
proof of concept pilot study: prevalence of grass virus infection and the potential for effects on the allergenic potency of pollen.wild plants harbour a variety of viruses and these have the potential to alter the composition of pollen. the potential consequences of virus infection of grasses on pollen-induced allergic disease are not known.200920102577
cocksfoot mottle sobemovirus coat protein contains two nuclear localization signals.cocksfoot mottle virus (cfmv) coat protein (cp) localization was studied in plant and mammalian cells. fusion of the full-length cp with enhanced green fluorescent protein (egfp) localized to the cell nucleus whereas similar constructs lacking the first 33 n-terminal amino acids of cp localized to the cytoplasm. cp and egfp fusions containing mutations in the arginine-rich motif of cp localized to the cytoplasm and to the nucleus in plant cells indicating the involvement of the motif in nuclear ...201020155311
effects of spontaneous heating on forage protein fractions and in situ disappearance kinetics of crude protein for alfalfa-orchardgrass hays packaged in large round bales.during 2006 and 2007, forages from 3 individual hay harvests were used to assess the effects of spontaneous heating on concentrations of crude protein (cp), neutral detergent insoluble cp (ndicp), acid detergent insoluble cp (adicp), and in situ disappearance kinetics of cp and ndicp for large round bales of mixed alfalfa (medicago sativa l.) and orchardgrass (dactylis glomerata l.). over the 3 harvests, 96 large round bales were made at preset bale diameters of 0.9, 1.2, or 1.5m and at moisture ...201020172236
[examination about positive ratio of pollen antigens by scratch test].japanese cedars and grass are famous for causing pollen allergy with antigen. but recently, many other antigens causing pollen allergy are coming to the front. we investigated positive ratios of pollen antigens of the consultation patients.200920220303
detection of cereal yellow dwarf virus using small interfering rnas and enhanced infection rate with cocksfoot streak virus in wild cocksfoot grass (dactylis glomerata).small rna sequences were obtained from leaf extracts of wild dactylis glomerata (cocksfoot grass) using deep sequencing (454 life sciences, roche diagnostics), and were screened against virus sequences in genbank using a local blastn search program (bioedit). putative small interfering (si)rnas complementary in sequence to cereal yellow dwarf virus (cydv, genus luteovirus) genomes were identified. primer sequences were made against the "high scoring" sirna sequences and rt-pcr was used to amplif ...201020558208
ultraviolet-absorbing compounds in milk are related to forage polyphenols.the aim of this work was to characterize uv-absorbing compounds (uac) in milk in relation to diet. in winter, 4 groups of cows each received a different diet: concentrate rich containing 35% cocksfoot hay (cch), maize silage (ms), rye grass silage (rs), or rye grass hay (rh). in summer, 2 additional diets were given: mountain grassland hay (gh) and mountain grassland pasture (gp). polyphenols were analyzed by hplc and folin reaction on forages and uac were extracted from milks and analyzed by hp ...201020630201
effects of spontaneous heating on estimates of total digestible nutrients for alfalfa-orchardgrass hays packaged in large round bales.large round or large square hay packages are more likely to heat spontaneously during storage than hay packaged in conventional (45 kg) bales, and the effects of this phenomenon on the associated energy estimates for these hays can be severe. our objectives for this project were to assess the relationship between estimates of total digestible nutrients (tdn) and spontaneous heating and to describe any important differences in energy estimates that may result specifically from 2 methods of estima ...201020630254
which allergen extract for grass pollen immunotherapy? an in vitro study.grass pollen is a major cause of allergy throughout the world. the only treatment targeting the causes and not only the symptoms of allergy is specific immunotherapy (it). a number of controlled trials demonstrated the efficacy of it in grass pollen allergic subjects, most using extracts of multiple grasses but some using extracts of a single grass. the optimal grass extract for it has not yet been established.201020653430
uptake and transformation of soil [14c]-trinitrotoluene by cool-season grasses.this study investigated the fate and uptake of [(14)c]-tnt from soil into orchardgrass (dactylis glomerata), perennial ryegrass (lolium perenne), and tall fescue (festuca arundinacea) over a one year period in a greenhouse-controlled environment. pots (n = 4 for each grass, containing 10 mg cold tnt/kg soil + 1.2 mg [(14)c]-tnt/kg soil and controls with no tnt) were exposed to light and temperature conditions typical of june at 45 degrees n for 369 days. three plant harvests were made (63, 181, ...201020666491
plant resource-use strategies: the importance of phenotypic plasticity in response to a productivity gradient for two subalpine species.functional traits are indicators of plant interactions with their environment and the resource-use strategies of species can be defined through some key functional traits. the importance of genetic variability and phenotypic plasticity in trait variations in response to a common environmental change was investigated in two subalpine species.201020682576
enzymatic activity of a mine soil varies according to vegetation cover and level of compost applied.we applied three doses of compost from mixed municipal solid waste (0, 15, and 30 g kg(-1) of soil) to a soil developed on pyrite mine wastes. part of the soil was planted with young erica australis l. collected at the mine; part was fertilized with n-p-k-mg and sown with dactylis glomerata l .bare soil without mineral fertilization was included in the experiment, as well. compost application to bare soil increased ph, provided plant nutrients, and enhanced the activity of the six soil enzymes t ...201020734914
bridging allergologic and botanical knowledge in seasonal allergy: a role for phenology.grass pollen is a worldwide cause of respiratory allergy. identifying the causative species is essential, for example for choosing the appropriate immunotherapy, because not all grass allergens are totally cross-reacting, and the pollen calendars provide only a gross estimate. phenologic analyses allow identification of the pollen release for each individual grass.201020800789
root-shoot growth responses during interspecific competition quantified using allometric modelling.plant competition studies are restricted by the difficulty of quantifying root systems of competitors. analyses are usually limited to above-ground traits. here, a new approach to address this issue is reported.201020829193
the effects of endophytes on seed production and seed predation of tall fescue and meadow fescue.fungal endophytes of grasses are often included in agricultural management and in ecological studies of natural grass populations. in european agriculture and ecological studies, however, grass endophytes are largely ignored. in this study, we determined endophyte infection frequencies of 13 european cultivars and 49 wild tall fescue (schedonorus phoenix) populations in northern europe. we then examined seed production and seed predation of endophyte-infected (e+) and endophyte-free (e-) tall fe ...201020871988
two new species of rhynchosporium.rhynchosporium consists of two species, r. secalis and r. orthosporum. both are pathogens of grasses with r. secalis infecting a variety of poaceae hosts and r. orthosporum infecting dactylis glomerata. phylogenetic analyses of multilocus dna sequence data on r. secalis isolates originating from cultivated barley, rye, triticale and other grasses, including agropyron spp., bromus diandrus and hordeum spp., resolved the monophyletic groups into three species according to their respective hosts. h ...201120943529
effects of cytoplasm and gene dosage on expression of male sterility in dactylis glomerata. 194621066350
protein-rna linkage and post-translational modifications of two sobemovirus vpgs.sobemoviruses possess a viral genome-linked protein (vpg) attached to the 5' end of viral rna. vpg is processed from the viral polyprotein. in the current study, cocksfoot mottle virus (cfmv) and rice yellow mottle virus (rymv) vpgs were purified from virions and analysed by mass spectrometry. the cleavage sites in the polyprotein and thereof the termini of vpg were experimentally proven. the lengths of the mature vpgs were determined to be 78 and 79 aa residues, respectively. the amino acid res ...201021068217
do sheep (ovis aries) categorize plant species according to botanical family?the ability of grazing herbivores to assign food types to categories by relying on certain relevant criteria could considerably reduce cognitive demand and increase their foraging efficiency when selecting among many different plant items. grasses and legumes differ functionally in vegetation communities as well as in nutritive value. we aimed to determine whether sheep can generalize an aversion they learnt for a grass or a legume species to another species of the same functional type and conse ...201121203791
nucleotide bias of dcl and ago in plant anti-virus gene silencing.plant dicer-like (dcl) and argonaute (ago) are the key enzymes involved in anti-virus post-transcriptional gene silencing (av-ptgs). here we show that av-ptgs exhibited nucleotide preference by calculating a relative av-ptgs efficiency on processing viral rna substrates. in comparison with genome sequences of dicot-infecting turnip mosaic virus (tumv) and monocot-infecting cocksfoot streak virus (csv), viral-derived small interfering rnas (vsirnas) displayed positive correlations between av-ptgs ...201021203927
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