Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| temporal and light regulation of the expression of three phytochromes in germinating seeds and young seedlings of avena sativa l. | an oat (avena sativa l.) plant contains at least three phytochromes, which have monomeric masses of 125, 124, and 123 kilodaltons (kda) (wang et al., 1991, planta 184, 96-104). the 124-kda phytochrome is most abundant in dark-grown seedlings, while the other two phytochromes predominate in light-grown seedlings. using three monoclonal antibodies, each specific to one of the three phytochromes, we have monitored by immunoblot assay the expression of these three phytochromes in the 5 d following o ... | 1993 | 24178495 |
| proton efflux from oat coleoptile cells and exchange with wall calcium after iaa or fusicoccin treatment. | elongation growth of plant cells occurs by stretching of cell walls under turgor pressure when intermolecular bonds in the walls are temporarily loosened. the acid-growth theory predicts that wall loosening is the result of wall acidification because treatments (including iaa and fusicoccin) that cause lowered wall ph cause elongation. however, conclusive evidence that iaa primarily reduces wall ph has been lacking. calcium has been reported to stiffen the cell walls. we have used a microelectro ... | 1993 | 24178494 |
| calcium antagonists inhibit sustained gibberellic acid-induced growth of avena (oat) stem segments. | the elongation response of avena sativa (oat) stem segments to gibberellic acid (ga3) is of large magnitude, with high hormonal sensitivity and specificity, but without cell division activity. this system is therefore an excellent model for mechanistic studies on higher plant cell elongation and the action of gibberellin. at millimolar concentrations, the calcium antagonists verapamil, d-600, nicardipine, diltiazem, bepridil, 8-(n,n,-diethylamino)-octyl-3,4,5-trimethoxybenzoate hcl, and lanthanu ... | 1993 | 12231695 |
| β-glucuronidase gene expression and mrna stability in oat protoplasts. | protoplasts derived from oat (avena sativa l.) suspension culture cells (7 days after subculturing) were electroporated with plasmid dna containing the escherichia coli uida gene encoding the ß-glucuronidase reporter enzyme. consistently high enzyme activity was observed with electroporation conditions of 500 μf and 1125 volts/cm. enzyme activity and mrna accumulation time courses were determined. the maximum enzyme activity was detected at 24 hours after electroporation, while the maximum mrna ... | 1993 | 24197350 |
| chaperonin-mediated reconstitution of the phytochrome photoreceptor. | the native phytochrome photoreceptor was purified to homogeneity from etiolated seedlings of oat (avena sativa l.) and used for renaturation experiments. light scattering measurements showed that the groel molecular chaperone interacts with non-native phytochrome to suppress aggregation of the refolding polypeptide, following its dilution from a chaotrope. the binary complex formed between non-native phytochrome and groel was stable and could be isolated by size exclusion chromatography. dischar ... | 1993 | 8095267 |
| two tcp-1-related but highly divergent gene families exist in oat encoding proteins of assumed chaperone function. | tcp-1-related sequences have been isolated from a cdna library of etiolated 6-day-old oat (avena sativa) seedlings. this attempt was made to obtain cdnas of a recently published 60 kda plant chaperone that re-folds denatured phytochrome and which was biochemically characterised as a tcp-1-related protein [(1993) nature 363, 644-647]. the translation of the putative coding sequence from one full-length cdna clone displays no specific homologies to amino acid sequences known from peptide sequencin ... | 1993 | 7903257 |
| [a comparative ecological-genetic assessment of the mutagenic background in 2 agricultural districts of the transcarpathian province]. | the estimation of the total mutagenic overloading and chromosome variability in population of two transcarpathian regions in ukraine differing in the intensity of pesticides use has been studied. the statistically significant differences in mutagenicity of soil (on the growing seed allium cepa), mutability of agricultural crops (vicia sativa, avena sativa) and chromosome aberrations level in peripheral lymphocytes in juveniles have been determined. | 1993 | 8316952 |
| in situ localization of faba bean and oat legumin-type proteins in transgenic tobacco seeds by a highly sensitive immunological tissue print technique. | we have used a highly sensitive immunological tissue print technique to study cell- and tissue-specific expression of heterologous genes in transgenic plants. primary polyclonal antibodies, raised against legumin of faba bean (vicia faba l.) and 12s globulin of oat (avena sativa l.) were used to localize these proteins in transgenic tobacco seeds in a streptavidin-alkaline phosphatase assay in combination with biotinylated secondary antibodies producing a higher sensitivity (by several amplifica ... | 1993 | 8400128 |
| expression of phytochrome apoprotein from avena sativa in escherichia coli and formation of photoactive chromoproteins by assembly with phycocyanobilin. | phytochrome dnas from oat (avena sativa l.) encoding the full-length 124-kda polypeptide, a 118-kda fragment lacking the first 65 amino acids, and two n-terminal fragments of 65 kda and 45 kda were subcloned and expressed in escherichia coli. reducing the temperature to 25 degrees c during cell growth and the coexpression of chaperones improved the folding into a functional conformation for most of the polypeptides, and in one case the yield of polypeptides was also enhanced. a maximum yield of ... | 1994 | 8033910 |
| effects of early experience on voluntary intake of low-quality roughage by sheep. | this study determined 1) how experience early in life with cured weeping lovegrass (eragrostis curvula) (low-quality roughage) or fresh oats (avena sativa) (high-quality roughage) affected consumption of low- or high-quality roughage by lambs later in life and 2) whether this early dietary experience affected passage of undigested residues through the digestive tract and digestion of low-quality roughage. from 1 to 5 mo of age, experienced lambs (el) were grazing cured weeping lovegrass, whereas ... | 1994 | 8056663 |
| purification and characterization of an alpha-l-arabinofuranosidase from streptomyces lividans 66 and dna sequence of the gene (abfa). | the gene encoding an alpha-l-arabinofuranosidase (abfa) was homologously cloned in streptomyces lividans and its dna sequence was determined. the enzyme was purified from the cytoplasm of the hyperproducing clone s. lividans iaf116. its m(r) was estimated by gel filtration and found to be approx. 380,000. since sds/page indicated a native protein of m(r) 69,000, it can be concluded that the native protein consists of several subunits of that size. the pi value was 4.6. the kinetic constants dete ... | 1994 | 8092996 |
| evidence that the alpha a gene of barley stripe mosaic virus encodes determinants of pathogenicity to oat (avena sativa). | complementary dna clones from which infectious rna can be transcribed were used to map the genetic determinants for oat (avena sativa l.) pathogenicity of barley stripe mosaic virus (bsmv). pseudorecombinant analysis of bsmv strains nd18 (nonpathogenic to oat) and cv42 (pathogenic to oat) indicated that the ability to systemically infect oat mapped to rna alpha. a homologous recombinant of nd18 (18 alpha tktkin), possessing nucleotides 2218 to 2454 from cv42 rna alpha, induced symptoms on oat si ... | 1994 | 8178475 |
| restriction fragment length polymorphisms linked to genes for resistance to crown rust (puccinia coronata) in near-isogenic lines of hexaploid oat (avena sativa). | crown rust, perhaps the most important fungal disease of oat, is caused by puccinia coronata. an examination of near-isogenic lines (nils) of hexaploid oat (avena sativa) was conducted to identify markers linked to genes for resistance to crown rust. these lines were created such that a unique resistance gene is present in each of the two recurrent parent backgrounds. the six nils of the current study, x434-ii, x466-i, and y345 (recurrent parent c237-89) and d486, d494, and d526 (recurrent paren ... | 1994 | 18470125 |
| interactions between heterodera avenae and pratylenchus neglectus on wheat. | in a long-term field experiment, differential population densities of heterodera avenae were produced by frequent cropping with resistant (cv. panema) or susceptible (cv. peniarth) oat. the two oat cultivars were equally good hosts of pratylenchus neglectus in a glass house experiment with field soil. on wheat crops grown after oats in field experiments, p. neglectus population densities in roots were higher in plots where h. avenae had been controlled than in plots with moderate infestations (4 ... | 1994 | 19279901 |
| the identification of random amplified polymorphic dna markers for daylength insensitivity in oat. | daylength insensitive accessions of avena sativa l. are being used to develop cultivars that will flower normally when grown under short or long photoperiods. field data indicate that the insensitivity trait is under the control of a single dominant gene, designated di1. the random amplified polymorphic dna (rapd) technique and bulk segregant analysis of daylength sensitive and insensitive plants were used to find markers for this gene. five of 200 random decamer primers tested produced polymorp ... | 1994 | 18470134 |
| avenacosidase from oat: purification, sequence analysis and biochemical characterization of a new member of the bga family of beta-glucosidases. | a protein consisting of 60 kda subunits (as-p60) was isolated from etiolated oat seedlings (avena sativa l.) and characterized as avenacosidase, a beta-glucosidase that belongs to a preformed defence system of oat against fungal infection. the enzyme is highly aggregated; it consists of 300-350 kda aggregates and multimers thereof. dissociation by freezing/thawing leads to complete loss of enzyme activity. the specificity of the enzyme was investigated with para-nitrophenyl derivatives which ser ... | 1994 | 8000004 |
| the complete amino acid sequence and disulphide bond arrangement of oat alcohol-soluble avenin-3. | we have elucidated the complete amino acid sequence of one of the avenin components, avenin-3, isolated from oat (avena sativa l.), variety narymsky 943. the sequence of the protein was determined by sequencing of cnbr and trypsin-generated peptides in combination with mass spectrometry. the protein is a single polypeptide chain, consisting of 201 amino acid residues with m(r) 23,252.8. the n-terminal amino acid residue of the protein is blocked with 5-oxoproline (pyroglutamic acid). all eight c ... | 1994 | 7925380 |
| genomic in situ hybridization in avena sativa. | genomic fluorescent in situ hybridization was employed in the study of the genome organization and evolution of hexaploid oat (avena sativa l. cv. sun ii, aaccdd, 2n = 6x = 42). genomic dnas from two diploid oat species, avena strigosa (genomic constitution asas, 2n = 14) and avena pilosa (genomic constitution cpcp, 2n = 14), were used as probes in the study. the dna from a. strigosa labelled 28 of the 42 (2/3) chromosomes of the hexaploid oat, while 14 of the 42 (1/3) chromosomes were labelled ... | 1994 | 18470104 |
| purification of the fusicoccin-binding protein from oat root plasma membrane by affinity chromatography with biotinylated fusicoccin. | fusicoccin (fc), a fungal phytotoxin, evokes a number of physiological responses after binding to the fc-binding protein (fcbp). for characterization of this plasma membrane protein and elucidation of the signal transduction pathway, we purified active fcbp from oat (avena sativa l. cv valiant) root plasma membranes using avidin-biotin affinity chromatography. for the binding of fcbp to immobilized avidin, a bifunctional fc derivative (fc-biotin, fcbio) was synthesized. fcbio retained high bindi ... | 1994 | 12232284 |
| purification of a membrane-bound udp-glucose:sterol [beta]-d-glucosyltransferase based on its solubility in diethyl ether. | membrane-bound udp-glucose:sterol [beta]-d-glucosyltransferase (udpg-sgtase) catalyzes the formation of steryl glucosides from udp-glucose and free sterols. this enzyme was purified from etiolated oat shoots (avena sativa l. cv alfred) in five steps. udpg-sgtase was solubilized from a microsomal fraction with the detergent n-octyl-[beta]-d-thioglucopyranoside and then extracted into diethyl ether. subsequent removal of the organic solvent, resolubilization with an aqueous buffer, and two column ... | 1994 | 12232266 |
| the structure of the rdna intergenic spacer of avena sativa l.: a comparative study. | the sequence of the 18s-25s ribosomal rna gene intergenic spacer (igs) of avena sativa was determined. dna was cloned after polymerase chain reaction amplification of the igs. the spacer of 3980 bp is composed of non-repeated sequences and five tandem arrays of repeated sequences, named a to e. homology between oats igs and other grass species was found. tandem arrays d and e seem to be originated by duplication from single-copy sequences in related species. a transcription initiation site and p ... | 1994 | 8061326 |
| the amino acid sequence previously attributed to a protein kinase or a tcp1-related molecular chaperone and co-purified with phytochrome is a beta-glucosidase. | a 60 kda protein (p60) co-purified with phytochrome was identified as avenacosidase, a beta-glucosidase which is part of the defense system of avena sativa. an antiserum raised against p60 was used to isolate a cdna clone coding for the complete amino acid sequence of p60. the cdna-derived amino acid sequence contained the partial sequences described before for a protein kinase [(1989) planta 178, 199-206] and for a tcp1-related molecular chaperone [(1993) nature 363, 644-647] co-purified with p ... | 1994 | 8013661 |
| ecogeographical distribution and differential adaptedness of multilocus allelic associations in spanish avena sativa l. | we determined the nine-locus isozyme genotype of 267 landrace accessions of avena sativa from 31 provinces of spain. our results establish that level of genetic variability is usually high both within and among accessions of this heavily self-fertilizing hexaploid grass and that multilocus genetic structure differs in various ecogeographical regions of spain. we concluded that selection favoring different multilocus genotypes in different environments was the main integrating force that shaped t ... | 1994 | 24185882 |
| modulation of h+-atpase activity by fusicoccin in plasma membrane vesicles from oat (avena sativa l.) roots (a comparison of modulation by fusicoccin, trypsin, and lysophosphatidylcholine). | the fungal phytotoxin fusicoccin affects various transport processes in the plasma membrane of plant cells. the plasma membrane (pm) h+-atpase (ec 3.6.1.35) seems to be the primary target of fusicoccin action. the kinetics of the stimulation of the pm h+-atpase by fusicoccin was studied in pm vesicles isolated from oat (avena sativa cv adamo) roots by aqueous two-phase partitioning. considerable stimulation of activity was observed only when roots were treated with fusicoccin prior to the pm iso ... | 1994 | 12232167 |
| allelopathy of oats. ii. allelochemical effect ofl-tryptophan and its concentration in oat root exudates. | l-tryptophan caused growth inhibition of roots and hypocotyls (or coleoptiles) of cockscomb (amaranthus caudatus l.), lettuce (lactuca sativa l.), cress (lepidium sativum l.), timothy (phleum pratense l.), rice (oryza sativa l.), wheat (triticum aestivum l.), and oat (avena sativa l.), increasing the dose ofl-tryptophan increased the inhibition. the concentrations for 50% inhibition of the root growth were 0.14, 0.15, 0.21, 0.79, 0.95, 1.7, and 2.4 mm for cockscomb, cress, lettuce, timothy, rice ... | 1994 | 24242057 |
| allelopathy of oats. i. assessment of allelopathic potential of extract of oat shoots and identification of an allelochemical. | the allelopathic potential of oat (avena sativa l.) extracts was investigated under laboratory conditions. the ethyl ether-, acetone-, and water-soluble fractions obtained from the extract of oat shoots inhibited the germination and growth of roots and hypocotyls of lettuce (lactuca sativa l.). the inhibitory activity of the water-soluble fraction was maximum, followed by that of ethyl ether-soluble and acetone-soluble fraction. an active principle of the water-soluble fraction was isolated and ... | 1994 | 24242056 |
| chromosomal localization and polymorphisms of ribosomal dna in oat (avena spp.). | the 17s/5.8s/26s ribosomal dna (rdna) sequences were mapped to the three satellited (sat) chromosomes in the common hexaploid cultivated oat avena sativa (2n = 6x = 42, aaccdd genomes). in situ hybridization and southern hybridization of maize and (or) wheat rdna probes to dna from nullisomics derived from the cultivar 'sun ii' allowed the placement of rdna sequences to the physical chromosomes. a restriction map was produced for the rdna sequences of 'sun ii' using a maize probe from the transc ... | 1994 | 18470058 |
| a contrast of the plasma membrane lipid composition of oat and rye leaves in relation to freezing tolerance. | the lipid composition of the plasma membrane isolated from leaves of spring oat (avena sativa l. cv ogle) was vastly different from that of winter rye (secale cereale l. cv puma). the plasma membrane of spring oat contained large proportions of phospholipids (28.8 mol% of the total lipids), cerebrosides (27.2 mol%), and acylated sterylglucosides (27.3 mol%) with lesser proportions of free sterols (8.4 mol%) and sterylglucosides (5.6 mol%). in contrast, the plasma membrane of winter rye contained ... | 1994 | 12232097 |
| a comparison of freezing injury in oat and rye: two cereals at the extremes of freezing tolerance. | a detailed analysis of cold acclimation of a winter rye (secale cereale l. cv puma), a winter oat (avena sativa l. cv kanota), and a spring oat cultivar (ogle) revealed that freezing injury of leaves of nonacclimated seedlings occurred at -2[deg]c in both the winter and spring cultivars of oat but did not occur in winter rye leaves until after freezing at -4[deg]c. the maximum freezing tolerance was attained in all cultivars after 4 weeks of cold acclimation, and the temperature at which 50% ele ... | 1994 | 12232096 |
| exposure of oats, avena sativa l., to filtered and unfiltered air in open-top chambers: effects on grain yield and quality. | field grown oats, avena sativa l. cv vital, were exposed to filtered and unfiltered air from anthesis until harvest in open-top chambers at a site in south-west sweden. ambient plots were used to study the influence of the chamber itself. with the exception of the number of grains per panicle, which was significantly higher in the charcoal-filtered treatment, no significant filtration effects were obtained for any of the plant growth parameters studied), i.e. grain yield, number of panicles per ... | 1994 | 15091629 |
| comparative mapping in grasses. oat relationships. | the development of rflp linkage maps in hexaploid and diploid oat allows us to study genetic relationships of these species at the dna level. in this report, we present the extension of a previously developed diploid oat map (avena atlantica x a. hirtula) and its molecular-genetic relationships with wheat, rice and maize. examination of 92-99% of the length of the oat genome map with probes common to triticeae species, rice or maize showed that 84, 79 and 71%, respectively, was conserved between ... | 1995 | 7500960 |
| arginine decarboxylase is localized in chloroplasts. | plants, unlike animals, can use either ornithine decarboxylase or arginine decarboxylase (adc) to produce the polyamine precursor putrescine. lack of knowledge of the exact cellular and subcellular location of these enzymes has been one of the main obstacles to our understanding of the biological role of polyamines in plants. we have generated polyclonal antibodies to oat (avena sativa l.) adc to study the spatial distribution and subcellular localization of adc protein in different oat tissues. ... | 1995 | 12228631 |
| levels of a terpenoid glycoside (blumenin) and cell wall-bound phenolics in some cereal mycorrhizas. | four cereals, hordeum vulgare (barley), triticum aestivum (wheat), secale cereal (rye), and avena sativa (oat), were grown in a defined nutritional medium with and without the arbuscular mycorrhizal fungus glomus intraradices. levels of soluble and cell wall-bound secondary metabolites in the roots of mycorrhizal and nonmycorrhizal plants were determined by high-performance liquid chromatography during the first 6 to 8 weeks of plant development. whereas there was no difference in the levels of ... | 1995 | 7480342 |
| fourier-transform resonance raman spectroscopy of intermediates of the phytochrome photocycle. | the parent states of the 124-kda phytochrome (phy a from avena sativa) and intermediates of its photocycle were studied by low-temperature fourier-transform resonance raman spectroscopy. spectra of the primary photoproducts i700 and lumi-f and of the thermal intermediate meta-f have been obtained for the first time. the spectra of the stable photochromic forms of photochrome, pr and pfr, presented in this work are significantly better in signal-to-noise ratio and resolution than previously publi ... | 1995 | 7654704 |
| the genomic identification of some monosomics of avena sativa l. cv. sun ii using genomic in situ hybridization. | genomic in situ hybridization using total genomic dna extracted from the c genome diploid species avena eriantha (2n = 2x = 14, genome cpcp) was used to identify monosomics (2n = 6x − 1 = 41) of the constituent genomes of the hexaploid cultivated oat a. sativa l. cv. sun ii (2n = 6x = 42, genomes aaccdd). the results demonstrate 3 ad/c and 6 c/ad chromosome translocations, indicate that five of the missing monosomics are derived from the c genome, and show that there are duplicates within ... | 1995 | 18470201 |
| characterization of a library from a single microdissected oat (avena sativa l.) chromosome. | a plasmid library of oat chromosome no21, the smallest chromosome of the complement, was constructed by microdissection and microcloning. the chromosome was deproteinized with proteinase k and digested with sau3a and linker adaptors were ligated to the dna fragments. from the single chromosome (less than 0.4 pg), 10 μg of dna was obtained after 2 rounds of pcr amplification. cloning experiments with the amplified dna produced as many as 500 000 recombinant clones from the single chromosome. t ... | 1995 | 18470198 |
| genomic clones encoding 11s globulins in oats (avena sativa l.). | we have isolated two complete genomic clones, glav1 and glav3, encoding 11s globulins (legumins) in oat. the structure of glav1 deviates from that of the typical legumin gene. this clone possesses an extra intron and an extra exon that is composed entirely of repeats of sequences found elsewhere in the clone. if this exon is functional, the protein encoded by glav1 will contain novel octapeptide and hendecapeptide repeats. the two glav clones show stronger and more extensive homology with one an ... | 1995 | 7672598 |
| inheritance and linkage relationships of seed peroxidase loci in hexaploid oat (avena sativa l.). | polyacrylamide gel electrophoresis has been used to investigate the inheritance and linkage relationships between anodal (pxa) and cathodal (pxc) seed peroxidases in hexaploid oat (avena sativa l.). a total of 12 seed peroxidase loci (5 loci of pxa and 7 loci of pxc) were identified in three crosses. only two pxc loci (pxc5 and pxc7) were not linked to any peroxidase loci; the others were scored in three linkage groups. the order of the three loci assigned to one of the linkage groups was pxc1-p ... | 1995 | 18470183 |
| a molecular linkage map of cultivated oat. | a molecular linkage map of cultivated oat composed of 561 loci has been developed using 71 recombinant inbred lines from a cross between avena byzantina cv. kanota and a. sativa cv. ogle. the loci are mainly restriction fragment length polymorphisms detected by oat cdna clones from leaf, endosperm, and root tissue, as well as by barley leaf cdna clones. the loci form 38 linkage groups ranging in size from 0.0 to 122.1 cm (mean, 39 cm) and consist of 2-51 loci each (mean, 14). twenty-nine loci re ... | 1995 | 18470176 |
| mgatp-dependent transport of phytochelatins across the tonoplast of oat roots. | in cd-exposed oat (avena sativa) roots cd was found to be associated primarily with the phytochelatin ([gamma]-glutamylcysteinyl)3-glutamic acid [([gamma]ec)3g], with a peptide to cd ratio of 1:3 (cysteine to cd ratio of 1:1), even though both ([gamma]ec)2g and ([gamma]ec)3g were present in the roots. phytochelatins are known to accumulate in the vacuoles of plant cells on exposure to cd, but the mechanism is not clear. here we present evidence for the transport of the phytochelatins ([gamma]ec) ... | 1995 | 12228436 |
| efficient callus formation and plant regeneration from leaves of oats (avena sativa l.). | an efficient plant regeneration system from leaf-derived callus in 6 genotypes of avena sativa l. has been established. regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings. plants were regenerated from the regenerable callus and grown to maturity. the frequency of regenerable callus formation and plant regeneration was correlated with the position, developmental stage and genotype of the expiant. the regeneration capacity of the first one mm of the leaf basal re ... | 1995 | 24185340 |
| oat leaf base: tissue with an efficient regeneration capacity. | an efficient short term regeneration system using seedling derived oat (avena sativa) leaf tissue has been developed. callus derived from the leaf base showed a higher response of plant regeneration than callus initiated from mesocotyls and more mature parts of the leaves. a correlation between the nuclear dna content of the donor material, as analysed with flow cytometry, and its ability to form callus was observed. somatic embryogenesis was histologically recognised from callus derived from ti ... | 1995 | 24185332 |
| gibberellin-regulated expression in oat aleurone cells of two kinases that show homology to map kinase and a ribosomal protein kinase. | cdna fragments from ten different protein kinases expressed in avena sativa aleurone cells were amplified from mrna by rt-pcr with degenerate primers. these could be classified into five groups: aspk1-3 showed homology to the snf1-related protein kinases, aspk4-5 to a wheat aba up-regulated protein kinase, aspk6-8 to the ca-dependent, calmodulin-independent protein kinase family, aspk9 encoded a map kinase and aspk10 was closely related to a novel arabidopsis ribosomal protein kinase. ga caused ... | 1995 | 7766874 |
| hormonal and gravitropic specificity in the regulation of growth and cell wall synthesis in pulvini and internodes from shoots of avena sativa l. (oat). | segments can be cut from the peduncular-1 internode of oat (avena sativa l.) shoots so as to contain the graviresponsive leaf-sheath pulvinus and gibberellin-sensitive internodal tissue. incorporation of [14c]glucose was used to monitor cell wall synthesis in these two tissues as affected by gravistimulus, indoleacetic acid (iaa), gibberellic acid (ga3), and fusicoccin (fc). pulvinar cell wall synthesis was promoted by iaa and fc (both within about 1 h), as well as by gravistimulus (starting bet ... | 1995 | 11536686 |
| basis for changes in the auxin-sensitivity of avena sativa (oat) leaf-sheath pulvini during the gravitropic response. | during the gravitropic response, auxin-sensitivity of the lower flanks of leaf-sheath pulvini of avena sativa (oat) is at least 1000-fold higher than those of the upper flanks and non-gravistimulated pulvini. when the pulvini are treated with 1 mm ca2+, a 10-fold increase in auxin-sensitivity of the pulvini is observed. related to this difference in auxin-sensitivity, in vitro activation of the vanadate-sensitive h(-)-atpase by iaa was observed. results show that the activation of the h(+)-atpas ... | 1995 | 11538414 |
| use of paromomycin as a selective agent for oat transformation. | friable, embryogenic oat (avena sativa l.) tissue cultures were stably transformed with two different plasmids containing the e. coli tn5 neomycin phosphotransferase ii gene (npt ii). selection was accomplished using the antibiotic paromomycin sulfate following microprojectile bombardment. from two independent experiments, 88 paromomycin-resistant tissue cultures were shown to be transgenic based on southern blot analysis and detection of the neomycin phosphotransferase (npt ii) protein using el ... | 1995 | 24194311 |
| pollen allergen homologues in barley and other crop species. | pollen from 10 agricultural plant species was surveyed for the presence of proteins crossreactive with group i, group iv and group ix allergens. barley (hordeum vulgare), maize (zea mays), rye (secale cerale), triticale (xtriticosecale cereale), oats (avena sativa), canola (brassica napus) and sunflower (helianthus annus) pollens contained numerous allergen cognate proteins. northern blot analysis of barley pollen rna revealed the presence of group i and group ix allergen transcripts. the barley ... | 1995 | 7537171 |
| persistence of the effects of early experience on consumption of low-quality roughage by sheep. | the aim of this study was to determine the effects of experience early in life with cured weeping lovegrass (eragrostis curvula) (low-quality roughage) or fresh oats (avena sativa) (high-quality roughage) on voluntary intake and nitrogen retention when sheep ate a low-quality roughage 9 mo after initial exposure. from 1 to 5 mo of age, experienced wethers (ew) grazed cured weeping lovegrass, whereas inexperienced wethers (iw) grazed fresh oats (initial exposure). then both ew and iw were fed sor ... | 1996 | 8726727 |
| isolation and expression of a host response gene family encoding thaumatin-like proteins in incompatible oat-stem rust fungus interactions. | four cdna clones (corresponding to tlp-1, -2, -3, and -4 genes) encoding thaumatin-like (tl), pathogenesis-related proteins were isolated from oat (avena sativa) infected by an incompatible isolate pga-1h of the oat stem rust fungus (puccinia graminis f. sp. avenae). all four cdna clones contained an open reading frame predicted to encode a 169-amino acid polypeptide with a signal peptide of 21 amino acids at the n-terminus, suggesting that these proteins are transported through a secretory path ... | 1996 | 8755626 |
| cloning and characterization of a highly repeated dna sequence in hordeum vulgare l. | a novel repetitive dna sequence, r10hvcop, has been identified in the barley (hordeum vulgare l.) genome. this 830 base pair (bp) dna sequence has a 606-bp open reading frame and is present as approximately 1.96 x 10(5) copies per haploid barley genome. southern blot analysis revealed that repetitive dna elements containing r10hvcop and related sequences were dispersed within the barley chromosomes. sequences similar to r10hvcop were also found in wheat (triticum aestivum l.), rye (secale cereal ... | 1996 | 8983185 |
| coleoptiles are gravi-guiding systems vital for gravi-insensitive shoots of germinating grass seedlings. | surgical removal of the coleoptiles from germinating seedlings of secale cereale l., avena sativa l., and zea mays l. leads to a total loss of gravitropic response of the shoot without affecting growth. the principal function of coleoptiles is therefore to guide the gravi-insensitive shoot to the soil surface. | 1996 | 11541944 |
| fourier-transform infrared spectroscopy of phytochrome: difference spectra of the intermediates of the photoreactions. | the photocycle of 124 kda phytochrome a from avena sativa was studied by fourier-transform infrared spectroscopy at low temperatures. difference spectra between the parent state pr and the intermediates of the pr-->pfr pathway, i.e. lumi-r, meta-ra, and meta-rc, and between pfr and the intermediates of the pfr-->pr pathway, lumi-f and meta-f, were obtained in 1h2o and 2h2o for the first time. each spectrum shows characteristic spectral features which allow a clear distinction between the differe ... | 1996 | 8718870 |
| cytological and molecular characterization of oat x maize partial hybrids. | in cereals, interspecific and intergeneric hybridizations (wide crosses) which yield karyotypically stable hybrid plants have been used as starting points to widen the genetic base of a crop and to construct stocks for genetic analysis. also, uniparental genome elimination in karyotypically unstable hybrids has been utilized for cereal haploid production. we have crossed hexaploid oat (2n=6x=42, avena sativa l.) and maize (2n=2x=20, zea mays l.) and recovered 90 progenies through embryo rescue. ... | 1996 | 24162209 |
| changes in blue-light-dependent protein phosphorylation during the early development of etiolated oat seedlings. | blue light induced the phosphorylation of a 116-kda plasma-membrane-associated protein in dark-grown seedlings from avena sativa l. the response was restricted to the phototropically sensitive tissue of the coleoptile tip. surprisingly, this protein showed different properties in membrane preparations from plants that were grown for 3 d than in those from 5-d-old seedlings. in contrast to the younger coleoptiles, in 5-d-old seedlings phosphorylation of the 116-kda protein depended strictly on th ... | 1996 | 11541069 |
| a calcium and free fatty acid-modulated protein kinase as putative effector of the fusicoccin 14-3-3 receptor. | a protein kinase that is activated by calcium and cis-unsaturated fatty acids has been characterized from oat (avena sativa l.) root plasma membranes. the kinase phosphorylates a synthetic peptide with a motif (-r-t-l-s-) that can be phosphorylated by both protein kinase c (pkc) and calcium-dependent protein kinase (cdpk)-type kinases. calphostin c and chelerythrine, two pkc inhibitors, completely inhibited the kinase activity with values of inhibitor concentration for 50% inhibition of 0.7 and ... | 1996 | 8754686 |
| suppression of gravitropic response of primary roots by submergence. | primary roots of six plant species were placed horizontally either in humid air or under water, and their growth and gravitropic responses were examined. in air, all the roots showed a normal gravitropic curvature. under water without aeration, roots of rice (oryza sativa l.), oat (avena sativa l.), azuki bean (vigna angularis ohwi et ohashi), and cress (lepidium sativum l.) curved downward at almost same rate as in air, whereas the curvature of roots of maize (zea mays l.) and pea (pisum sativu ... | 1996 | 11540722 |
| osmoregulation by oat coleoptile protoplasts (effect of auxin). | the effect of auxin on the physiology of protoplasts from growing oat (avena sativa l.) coleoptiles was investigated. protoplasts, isolated iso-osmotically from peeled oat coleoptile segments, were found to swell steadily over many hours. incubated in 1 mm cacl2, 10 mm kcl, 10 mm 2-(morpholino)ethanesulfonic acid/1,3-bis-[tris(hydroxymethyl)methylamino]propane, ph 6.5, and mannitol to 300 milliosmolal, protoplasts swelled 28.9% [plus or minus] 2.0 (standard error) after 6 h. addition of 10 [mu]m ... | 1996 | 12226237 |
| peroxygenase-catalyzed fatty acid epoxidation in cereal seeds (sequential oxidation of linoleic acid into 9(s),12(s),13(s)-trihydroxy-10(e)-octadecenoic acid). | peroxygenase-catalyzed epoxidation of oleic acid in preparations of cereal seeds was investigated. the 105,000g particle fraction of oat (avena sativa) seed homogenate showed high peroxygenase activity, i.e. 3034 [plus or minus] 288 and 2441 [plus or minus] 168 nmol (10 min)-1 mg-1 protein in two cultivars, whereas the corresponding fraction obtained from barley (hordeum vulgare and hordeum distichum), rye (secale cereale), and wheat (triticum aestivum) showed only weak activity, i.e. 13 to 138 ... | 1996 | 12226220 |
| infrared light-emitting diode radiation causes gravitropic and morphological effects in dark-grown oat seedlings. | oat (avena sativa cv seger) seedlings were irradiated with ir light-emitting diode (led) radiation passed through a visible-light-blocking filter. infrared led irradiated seedlings exhibited differences in growth and gravitropic response when compared to seedlings grown in darkness at the same temperature. thus, the oat seedlings in this study were able to detect ir led radiation. these findings call into question the use of ir led as a safe-light for some photosensitive plant response experimen ... | 1996 | 11536734 |
| purification and characterization of an oat fructan exohydrolase that preferentially hydrolyzes beta-2,6-fructans. | oat (avena sativa cv fulghum) fructan hydrolase was purified by ammonium sulfate precipitation and anion-exchange, hydrophobic interaction, and size-exclusion chromatography. the enzyme was purified to homogeneity as determined by the presence of a single band (43 kd) on a silver-stained sodium dodecyl sulfate-polyacrylamide gel. a mixture of beta-2,6-linked fructan (neokestin) isolated from oat was used as the substrate to purify fructan hydrolase. neokestin and small degree of polymerization f ... | 1996 | 8742337 |
| phosphoryl group exchange between atp and adp catalyzed by h+-atpase from oat roots. | atp-adp exchange was estimated in the presence of plasma membrane h+-atpase of oat (avena sativa) roots partially purified with triton x-100 by measuring [14c]atp formation from [14c]adp. most studies were done at 0[deg]c. at ph 6.0 the exchange showed: (a) mg2+ requirement with a biphasic response giving maximal activity at 152 [mu]m and (b) insensitivity to ionic strength, [na+], and [k+]. atp and adp dependence were analyzed with a model in which nucleotide-enzyme interactions are at rapid-ra ... | 1997 | 12223778 |
| aneuploid marker assignment in hexaploid oat with the c genome as a reference for determining remnant homoeology. | nullisomic lines of hexaploid oat avena sativa l. (2n = 6x - 2 = 40, aaccdd) cultivar sun ii were used to assign 134 dna sequences to 10 chromosome-associated syntenic groups. a limited set of ditelosomic lines allowed localization of subsets of these sequences to six chromosome arms. advantages of using such aneuploids in mapping are in the assignment of gene families, monomorphic rflp sequences, and oat linkage groups to chromosomes. the published hexaploid oat rflp linkage map has 38 linkage ... | 1997 | 9202416 |
| oat-maize chromosome addition lines: a new system for mapping the maize genome. | novel plants with individual maize chromosomes added to a complete oat genome have been recovered via embryo rescue from oat (avena sativa l., 2n = 6x = 42) x maize (zea mays l., 2n = 20) crosses. an oat-maize disomic addition line possessing 21 pairs of oat chromosomes and one maize chromosome 9 pair was used to construct a cosmid library. a multiprobe (mixture of labeled fragments used as a probe) of highly repetitive maize-specific sequences was used to selectively isolate cosmid clones conta ... | 1997 | 9108009 |
| gravitropism of oat and wheat coleoptiles: dependence on the stimulation angle and involvement of autotropic straightening. | gravitropism of oat (avena sativa l.) and wheat (triticum aestivum l.) coleoptiles was investigated in relation to the displacement angle or to the initially set stimulation angle (sa). we measured curvature rates at the early phase of curvature, before it was affected by the drop in sa resulting from the curvature response itself. the plot of the rates against the sines of initial sas revealed similar curves for oats and wheat, which approached saturation as the sine increased to unity. the two ... | 1997 | 11536867 |
| exposure of oat seedlings to blue light results in amplified phosphorylation of the putative photoreceptor for phototropism and in higher sensitivity of the plants to phototropic stimulation. | dark recovery of blue light-induced in vitro phosphorylation in oat (avena sativa l.) seedlings after in vivo preirradiation with blue light revealed different recovery kinetics for the coleoptile base and tip. although, in both cases, maximum in vitro phosphorylation was observed 90 min after in vivo blue light treatment, the phosphorylation levels for the entire base were about 3-fold higher than those found in nonpreirradiated plants. the tip response only slightly exceeded that of the dark c ... | 1997 | 11536818 |
| asymmetric, blue light-dependent phosphorylation of a 116-kilodalton plasma membrane protein can be correlated with the first- and second-positive phototropic curvature of oat coleoptiles. | the possible correlation between blue light-dependent phosphorylation of a 116-kd protein and phototropic responses of etiolated oat (avena sativa l.) seedlings was tested by a micromethod for protein phosphorylation. quantitation of the basipetal distribution of this protein showed that the in vitro 32p phosphorylation values declined exponentially from tip to node, with more than 50% of the total label being found in the uppermost 5 mm. nonsaturating preirradiation of the coleoptiles in vivo r ... | 1997 | 11536817 |
| inducible overexpression of oat arginine decarboxylase in transgenic tobacco plants. | to test the possible interaction of polyamines in plant growth responses, transgenic tobacco plants containing the avena sativa l. (oat) arginine decarboxylase (adc) gene under the control of a tetracycline-inducible promoter were generated. inducible overexpression of oat adc in transgenic tobacco led to an accumulation of adc mrna, increased adc activity and changes in polyamine levels. transgenic lines, induced during vegetative stage, displayed different degrees of an altered phenotype, the ... | 1997 | 9107036 |
| purification and characterization of recombinant affinity peptide-tagged oat phytochrome a. | full-length avena sativa (oat) phytochrome a (asphya) was expressed in the yeast saccharomyces cerevisiae and purified to apparent homogeneity. expression of an asphya cdna that encoded the full-length photoreceptor with a 15 amino acid 'strep-tag' peptide at its c-terminus produced a single polypeptide with a molecular mass of 124 kda. this strep-tagged polypeptide (asphya-st) bound tightly to streptavidin agarose and was selectively eluted using diaminobiotin, with a chromatographic efficiency ... | 1997 | 9114754 |
| theophylline metabolism in higher plants. | metabolism of [8-(14)c]theophylline was investigated in leaf segments from camellia sinensis (tea), camellia irrawadiensis, ilex paraguariensis (maté) and avena sativa, root segments of vigna mungo seedlings and cell suspension cultures of catharanthus roseus. there was extensive uptake and metabolism of [8-(14)c]theophylline by leaves of tea and camellia irrawadiensis and, to a lesser extent, maté. these purine alkaloid-containing species converted [8-(14)c]theophylline into 3-methylxanthine, x ... | 1997 | 9305805 |
| characterization of regions within the n-terminal 6-kilodalton domain of phytochrome a that modulate its biological activity. | phytochrome a (phya) is a red/far-red (fr) light photoreceptor responsible for initiating numerous light-mediated plant growth and developmental responses, especially in fr light-enriched environments. we previously showed that the first 70 amino acids of the polypeptide contain at least two regions with potentially opposite functions (e.t. jordan, j.r. cherry, j.m. walker, r.d. vierstra [1996] plant j 9: 243-257). one region is required for activity and correct apoprotein/chromophore interactio ... | 1997 | 9342873 |
| udp-glucose:sterol glucosyltransferase: cloning and functional expression in escherichia coli. | steryl glucosides are characteristic lipids of plant membranes. the biosynthesis of these lipids is catalyzed by the membrane-bound udp-glucose:sterol glucosyltransferase (ec 2.4.1.173). the purified enzyme (warnecke and heinz, plant physiol 105 (1994): 1067-1073) has been used for the cloning of a corresponding cdna from oat (avena sativa l.). amino acid sequences derived from the amino terminus of the purified protein and from peptides of a trypsin digestion were used to construct oligonucleot ... | 1997 | 9349281 |
| identification and characterization of a novel arabinoxylanase from wheat flour. | an endogenous wheat (triticum aestivum) flour endoxylanase was purified to homogeneity from a crude wheat flour extract by ammonium sulfate precipitation and cation-exchange chromatography. the 30-kd protein had an isoelectric point of 9.3 or higher. a sequence of 19 amino acids at the nh2 terminus showed 84.2% identity with an internal sequence of 15-kd grain-softness protein, friabilin. high-performance anion-exchange chromatography and gel-permeation analysis of the hydrolysis products indica ... | 1997 | 9414565 |
| ige antibodies to recombinant pollen allergens (phl p 1, phl p 2, phl p 5, and bet v 2) account for a high percentage of grass pollen-specific ige. | pollen from different grass species are some of the most potent elicitors of type i allergy worldwide. the characterization of antigenic structures and ige epitopes common to different grass species is relevant to define reagents for diagnosis and specific therapy of grass pollen allergy. | 1998 | 9500760 |
| herbicide safener-binding protein of maize. purification, cloning, and expression of an encoding cdna. | dichloroacetamide safeners protect maize (zea mays l.) against injury from chloroacetanilide and thiocarbamate herbicides. etiolated maize seedlings have a high-affinity cytosolic-binding site for the safener [3h](r,s)-3-dichloroacetyl-2,2,5-trimethyl-1, 3-oxazol-idine ([3h]saf), and this safener-binding activity (safba) is competitively inhibited by the herbicides. the safener-binding protein (safbp), purified to homogeneity, has a relative molecular weight of 39,000, as shown by sodium dodecyl ... | 1998 | 9501141 |
| the sugarcane bacilliform badnavirus promoter is active in both monocots and dicots. | regions of the sugarcane bacilliform badnavirus genome were tested for promoter activity. the genomic region spanning nucleotides 5999-7420 was shown to possess promoter activity as exemplified by its ability to drive the expression of the coding region of the uida gene of escherichia coli, in both avena sativa and arabidopsis thaliana. in a. sativa, the promoter was active in all organs examined and, with the exception of the anthers where the expression was localized, this activity was constit ... | 1998 | 9747843 |
| gibberellin-photoaffinity labelling of two polypeptides in plant plasma membranes. | two polypeptides of m(r) 68 kda and 18 kda were gibberellin (ga)-photoaffinity labelled in vitro in plasma membrane preparations from oat (avena sativa l.) aleurone and from leaves and stems of wild-type and ga-sensitivity mutants of different species. labelling of these polypeptides could be competed by biologically active, but not by inactive, gas, indicating the likely biological significance of these interactions. on 2-dimensional gels the radiolabelled polypeptides were each resolved as one ... | 1998 | 9750344 |
| central effects of ac-1 and tfg-1. | ac-1 and tfg-1 are both herb extracts from avena sativa and trigonella foenum graecum respectively. in folk medicine, trigonella foenum graecum is used as appetiser and general tonic as well as for pellagra treatment and for treatment of pulmonary disorders. avena sativa is another commonly used appetiser and tonic. in our study we examined the influence of ac-1 and tfg-1 on some of the major functions of the central nervous system. we divided the test animals in three groups and after administe ... | 1998 | 10206008 |
| identification of quantitative loci for tolerance to barley yellow dwarf virus in oat. | abstract molecular markers linked to quantitative trait loci conditioning tolerance to barley yellow dwarf virus (bydv) were identified in oat (avena sativa) using amplified fragment length polymorphism (aflp) analysis. near-isogenic and recombinant inbred lines (nils and rils, respectively) derived from a cross of clintland64 (bydv-sensitive) and il86-5698 (bydv-tolerant) were evaluated for their responses to an illinois isolate of the pav strain of bydv. individual markers identified in the an ... | 1998 | 18944919 |
| a microtechnique for the analysis of free and conjugated indole-3-acetic acid in milligram amounts of plant tissue using a benchtop gas chromatograph-mass spectrometer. | a microtechnique was developed for the quantification of indole-3-acetic acid (iaa) in plant samples of one milligram fresh weight or less. the method permitted quantification of both free and conjugated iaa using a benchtop gas chromatograph-mass spectrometer. new methods for sample purification with high recovery at microscale levels, together with simple changes that result in enhanced sensitivity of the instrumentation, allowed for a significant reduction in the amount of plant material requ ... | 1998 | 9443382 |
| crop rotation impacts on potato protein. | the impact of nitrogen (n) fertilization on potato (solanum tuberosum l.) protein yield and nutritional quality is well documented but of little benefit to growers with limited access to fertilizer or capital (e.g. in lesser developed countries). this study was conducted 1) to evaluate the extent which crude protein yield in potatoes can be influenced by crop rotation with no n fertilizer and 2) to determine if crop rotation and minimal application of n fertilizer can meet the total protein yiel ... | 1998 | 10426115 |
| nucleotide sequence of oat (avena sativa l.) cdna encoding an auxin-binding protein (abp1). | we isolated and determined a nucleotide sequence of a cdna clone encoding a protein homologous to maize major auxin-binding protein (abp1) from a cdna library of oat coleoptiles. the deduced amino acid sequence of this clone contained an n-linked glycosylation signal and an er-retention signal. furthermore, two domains that were important to interact with auxins, were conserved in this clone at amino acid level. | 1998 | 10520452 |
| chromosome-specific molecular organization of maize (zea mays l.) centromeric regions. | a set of oat-maize chromosome addition lines with individual maize (zea mays l.) chromosomes present in plants with a complete oat (avena sativa l.) chromosome complement provides a unique opportunity to analyze the organization of centromeric regions of each maize chromosome. a dna sequence, mcs1a, described previously as a maize centromere-associated sequence, was used as a probe to isolate cosmid clones from a genomic library made of dna purified from a maize chromosome 9 addition line. analy ... | 1998 | 9789043 |
| genetics of alpha-amylases in hexaploid oat species. | the inheritance of alpha-amylases was studied in six f2 populations of hexaploid oats (avena sativa, a. byzantina, a. fatua, a. sterillis) using polyacrylamide gel electrophoresis. a total of 22 loci was identified and described. three main linkages of four or five loci each and an additional two pairs of linked loci were detected. it seems likely that the three main linkage groups represent homeologous chromosomes. matching of alpha-amylase profiles of hexaploid (aaccdd), tetraploid (aacc), and ... | 1998 | 9775349 |
| discrimination of the closely related a and d genomes of the hexaploid oat avena sativa l. | a satellite dna sequence, as120a, specific to the a-genome chromosomes in the hexaploid oat, avena sativa l., was isolated by subcloning a fragment with internal tandem repeats from a plasmid, pas120, that had been obtained from an avena strigosa (as genome) genomic library. southern and in situ hybridization showed that sequences with homology to sequences within pas120 were dispersed throughout the genome of diploid (a and c genomes), tetraploid (ac genomes), and hexaploid (acd genomes) avena ... | 1998 | 9770506 |
| transgenic dna integrated into the oat genome is frequently interspersed by host dna. | integration of transgenic dna into the plant genome was investigated in 13 transgenic oat (avena sativa l.) lines produced using microprojectile bombardment with one or two cotransformed plasmids. in all transformation events, the transgenic dna integrated into the plant genome consisted of intact transgene copies that were accompanied by multiple, rearranged, and/or truncated transgene fragments. all fragments of transgenic dna cosegregated, indicating that they were integrated at single gene l ... | 1998 | 9770447 |
| irregular patterns of transgene silencing in allohexaploid oat. | an irregular pattern of transgene silencing was revealed in expression and inheritance studies conducted over multiple generations following transgene introduction by microprojectile bombardment of allohexaploid cultivated oat (avena sativa l.). expression of two transgenes, bar and uida, delivered on the same plasmid was investigated in 23 transgenic oat lines. twenty-one transgenic lines, each derived from an independently selected transformed tissue culture, showed expression of both bar and ... | 1998 | 9747805 |
| complex structure of knob dna on maize chromosome 9. retrotransposon invasion into heterochromatin. | the recovery of maize (zea mays l.) chromosome addition lines of oat (avena sativa l.) from oat x maize crosses enables us to analyze the structure and composition of specific regions, such as knobs, of individual maize chromosomes. a dna hybridization blot panel of eight individual maize chromosome addition lines revealed that 180-bp repeats found in knobs are present in each of these maize chromosomes, but the copy number varies from approximately 100 to 25, 000. cosmid clones with knob dna se ... | 1998 | 9691055 |
| isolation and structure of a new galactolipid from oat seeds. | seeds of oat (avena sativa l.) were recently shown to contain significant quantities of a new hydroxy acid, (15 r)-hydroxy-(9z),(12z)-octadecadienoic acid (trivial name, avenoleic acid). in the present work, avenoleate was found to be mainly (63%) localized in the glycolipid fraction of oat seed lipids. fractionation of the glycolipids by thin-layer chromatography and reversed-phase high-performance liquid chromatography revealed the presence of a main molecular species which accounted for 20% o ... | 1998 | 9590622 |
| association of nickel versus transport of cadmium and calcium in tonoplast vesicles of oat roots. | the plant vacuole has long been suspected of being a site for accumulation of ni in plant roots, but testing this hypothesis directly by vacuole isolation is technically difficult and has not been reported. here, we have attempted to determine if ni can be transported into isolated oat (avena sativa l.) root tonoplast vesicles as an alternative approach toward understanding the importance of the vacuole in ni accumulation in roots. we found that, in contrast to ca and cd, ni did not affect the p ... | 1998 | 9530882 |
| identification of a novel isoform of the chloroplast-coupling factor alpha-subunit. | studies were conducted to identify a 64-kd thylakoid membrane protein of unknown function. the protein was extracted from chloroplast thylakoids under low ionic strength conditions and purified to homogeneity by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. four peptides generated from the proteolytic cleavage of the wheat 64-kd protein were sequenced and found to be identical to internal sequences of the chloroplast-coupling factor (cf1) alpha-subunit. antibodies for th ... | 1998 | 9489017 |
| pigment-free nadph:protochlorophyllide oxidoreductase from avena sativa l. purification and substrate specificity. | the enzyme nadph:protochlorophyllide oxidoreductase (por) is the key enzyme for light-dependent chlorophyll biosynthesis. it accumulates in dark-grown plants as the ternary enzyme-substrate complex por-protochlorophyllide a-nadph. here, we describe a simple procedure for purification of pigment-free por from etioplasts of avena sativa seedlings. the procedure implies differential solubilization with n-octyl-beta-d-glucoside and one chromatographic step with deae-cellulose. we show, using pigment ... | 1999 | 10518779 |
| chromosomal organization of a sequence related to ltr-like elements of ty1-copia retrotransposons in avena species. | a repetitive sequence, pas17, was isolated from avena strigosa (as genome) and characterized. the insert was 646 bp in length and showed 54% at content. databank searches revealed its high homology to the long terminal repeat (ltr) sequences of the specific family of ty1-copia retrotransposons represented by wis2-1a and bare. it was also found to be 70% identical to the ltr domain of the wis2-1a retroelement of wheat and 67% identical to the bare-1 retroelement of barley. southern hybridizations ... | 1999 | 10464792 |
| antioxidant capacity of oat (avena sativa l.) extracts. 2. in vitro antioxidant activity and contents of phenolic and tocol antioxidants. | oat milling fractions were examined for concentrations of total phenolics, tocols, and phenolic acids and in vitro antioxidant activity to determine their potential as dietary antioxidants. methanolic extracts of pearling fractions, flour and aspirations from flaking, and trichomes had high, intermediate, and low antioxidant activities, respectively, evaluated by the beta-carotene bleaching method. pearling fractions were also highest in total phenolics and tocols. p-hydroxybenzoic acid, vanilli ... | 1999 | 10606549 |
| antioxidant capacity of oat (avena sativa l.) extracts. 1. inhibition of low-density lipoprotein oxidation and oxygen radical absorbance capacity. | milled oat groat pearlings, trichomes, flour, and bran were extracted with methanol and the fractions tested in vitro for antioxidant capacity against low-density lipoprotein (ldl) oxidation and r-phycoerythrin protein oxidation in the oxygen radical absorbance capacity (orac) assay. the oxidative reactions were generated by 2,2'-azobis(2-amidinopropane) hcl (aaph) or cu(2+) in the ldl assay and by aaph or cu(2+) + h(2)o(2) in the orac assay and calibrated against a trolox standard to calculate ... | 1999 | 10606548 |
| indole-3-acetic acid control on acidic oat cell wall peroxidases. | incubation of oat coleoptile segments with 40 µm indoleacetic acid (iaa) induced a decrease of 35-60% in peroxidase activity at the cell wall compartment. treatment with iaa also produced a similar decrease in the oxidation of nadh and iaa at the cell wall. isoelectric focusing of ionic, covalent, and intercellular wall peroxidase fractions showed that acidic isoforms (pi 4.0-5.5) were reduced preferentially by iaa treatment. marked differences were found between acidic and basic wall isoperoxid ... | 1999 | 10467016 |
| a photoperiod-insensitive barley line contains a light-labile phytochrome b | barley (hordeum vulgare l.) is a long-day plant whose flowering is enhanced when the photoperiod is supplemented with far-red light, and this promotion is mediated by phytochrome. a chemically mutagenized dwarf cultivar of barley was selected for early flowering time (barley maturity daylength response [bmdr]-1) and was made isogenic with the cultivar shabet (bmdr-8) by backcrossing. bmdr-1 was found to contain higher levels of both phytochrome a and phytochrome b in the dark on immunoblots with ... | 1999 | 10069841 |
| degradation of oat mrnas during seed development. | the genes av1, av10, and z1 encode proteins that accumulate during oat seed development. in developing endosperm of avena sativa (cultivated oat), av1, av10 and z1 mrnas reach maximal levels midway through seed development but fall to very low levels in mature seeds. similarly, mrnas for these proteins peak during endosperm development of avena fatua (wild oat) and are later degraded. however, during late maturation of a. fatua seeds, populations of mrna fragments shorter than the intact transcr ... | 1999 | 10350095 |
| usefulness of gene pg10 as a source of stem rust resistance in oat breeding. | abstract infection types produced by puccinia graminis f. sp. avenae on plants of avena sativa with the stem rust resistance gene pg10 are characterized by moderate-sized uredinia surrounded by an area of chlorosis and a larger variable zone of dark brown necrosis. this study was undertaken to assess the effectiveness of gene pg10 as a source of resistance to stem rust and to determine the interactions of this gene with other common pg genes. a derived pg10 line was tested with 58 distinct patho ... | 1999 | 18944647 |
| protein evaluation of four oat (avena sativa l.) cultivars adapted for cultivation in the south of brazil. | four oat cultivars adapted for soil and climate conditions in the southern region of brazil were evaluated for protein nutritive value. evaluations were done both in vitro and in vivo. in vitro evaluation was done by essential amino acid profile, available lysine, amino acid scoring, and protein digestibility corrected amino acid-scoring (pdcaas). nitrogen balance indices and per were determined in vivo with rats. in all four cultivars (ufp-15, ufp-16, ctc-03, ufrgs-14), lysine was the most limi ... | 1999 | 10540981 |
| reconstitution of a bacterial/plant polyamine biosynthesis pathway in saccharomyces cerevisiae. | polyamine synthesis in most organisms is initiated by the decarboxylation of ornithine to form putrescine via ornithine decarboxylase (odc). plants, some bacteria and some fungi and protozoa generate putrescine from arginine, via arginine decarboxylase (adc) and agmatine ureohydrolase (auh) or agmatine iminohydrolase. a polyamine-requiring strain of saccharomyces cerevisiae with a mutation in the gene encoding odc was transformed with plasmids bearing genes encoding escherichia coli adc and auh. ... | 1999 | 10075412 |